AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG003893

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003893 MtrunA17_Chr1g0191021 70.748 147 41 2 1 146 1 146 8.26e-56 179
MsaG003893 MtrunA17_Chr7g0273821 44.545 110 50 2 47 145 50 159 4.89e-21 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003893 AT3G04930.2 45.192 104 47 5 52 145 85 188 1.12e-17 78.6
MsaG003893 AT3G04930.1 45.192 104 47 5 52 145 85 188 1.48e-17 78.6
MsaG003893 AT5G28040.2 42.593 108 51 5 48 145 69 175 3.24e-15 71.6
MsaG003893 AT5G28040.1 42.593 108 51 5 48 145 69 175 3.24e-15 71.6
MsaG003893 AT1G44810.1 48.214 56 29 0 90 145 115 170 2.01e-12 63.2
MsaG003893 AT4G00390.1 36.735 98 45 2 59 146 116 206 6.89e-11 59.3

Find 30 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCCATTTGCTCCCATTT+TGG 0.158841 1:+63364555 None:intergenic
ACGACTCCCTTGAACCAAAA+TGG 0.241276 1:-63364569 MsaT003893.1:CDS
GGTGGTGGGGATGGTGGCAG+TGG 0.280876 1:+63364638 None:intergenic
GGTGGGGATGGTGGCAGTGG+TGG 0.286773 1:+63364641 None:intergenic
TCTTCTTATCATAATGATAC+TGG 0.348077 1:-63364432 MsaT003893.1:CDS
TTTGTTGTTGCAGCGGTGTT+TGG 0.389163 1:+63364600 None:intergenic
GATGGTGGCAGTGGTGGTGA+AGG 0.408033 1:+63364647 None:intergenic
TCAGCGTAATCGTCTTGATT+GGG 0.423484 1:+63364765 None:intergenic
GTCAGCGTAATCGTCTTGAT+TGG 0.437669 1:+63364764 None:intergenic
TTGACAAAGATTGTTCAGTT+TGG 0.444201 1:+63364670 None:intergenic
TGAACCAAAATGGGAGCAAA+TGG 0.444622 1:-63364559 MsaT003893.1:CDS
TAAGATGATGGTGGTGGGGA+TGG 0.445734 1:+63364629 None:intergenic
CGACTCCCTTGAACCAAAAT+GGG 0.455745 1:-63364568 MsaT003893.1:CDS
ATCGTTCTATGATCAAATTA+AGG 0.483787 1:-63364409 MsaT003893.1:CDS
CTTCTTTAGTCCAGAGACAT+TGG 0.490028 1:+63364505 None:intergenic
CTCGATTACAACGCCAATCG+TGG 0.500789 1:-63364456 MsaT003893.1:CDS
GATGATGGTGGTGGGGATGG+TGG 0.508919 1:+63364632 None:intergenic
CTTATCAACAAGCTAGCTCT+TGG 0.516531 1:+63364365 None:intergenic
CTCATGGCATTCTCTCGAAA+CGG 0.520102 1:-63364798 MsaT003893.1:CDS
AAGGCTAAGATGATGGTGGT+GGG 0.520564 1:+63364624 None:intergenic
GACGAGATTACGATCTTGCA+AGG 0.547606 1:-63364483 MsaT003893.1:CDS
TGTTGCAGCGGTGTTTGGTA+AGG 0.547867 1:+63364605 None:intergenic
TAAGGCTAAGATGATGGTGG+TGG 0.558629 1:+63364623 None:intergenic
GACGATTACGCTGACATCGA+AGG 0.560108 1:-63364756 MsaT003893.1:CDS
ATGATAAGAAGATCCACGAT+TGG 0.603760 1:+63364443 None:intergenic
CAGCGTAATCGTCTTGATTG+GGG 0.609306 1:+63364766 None:intergenic
GTTTGGTAAGGCTAAGATGA+TGG 0.620548 1:+63364617 None:intergenic
AGGCTAAGATGATGGTGGTG+GGG 0.634264 1:+63364625 None:intergenic
TGGTAAGGCTAAGATGATGG+TGG 0.656489 1:+63364620 None:intergenic
GATGATGTTTGTTGTTGCAG+CGG 0.689437 1:+63364593 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 63364377 63364817 63364377 ID=MsaG003893
Chr1 mRNA 63364377 63364817 63364377 ID=MsaT003893.1;Parent=MsaG003893
Chr1 exon 63364377 63364817 63364377 ID=MsaT003893.1.exon1;Parent=MsaT003893.1
Chr1 CDS 63364377 63364817 63364377 ID=cds.MsaT003893.1;Parent=MsaT003893.1
Gene Sequence

>MsaT003893.1

ATGGCATTCTCTCGAAACGGCATCGTTATCCCCAATCAAGACGATTACGCTGACATCGAAGGTGAAGACGATGACGAATTAGAAGAAGAAGACAACTATTTTGTCCAACCAAACATCAAAAACTACCAAACTGAACAATCTTTGTCAACCTTCACCACCACTGCCACCATCCCCACCACCATCATCTTAGCCTTACCAAACACCGCTGCAACAACAAACATCATCGACGACTCCCTTGAACCAAAATGGGAGCAAATGGAAGAGAAGAACAACGATTCACAGAAGCTTTTCCAATGTCTCTGGACTAAAGAAGACGAGATTACGATCTTGCAAGGTTTGCTCGATTACAACGCCAATCGTGGATCTTCTTATCATAATGATACTGGATCGTTCTATGATCAAATTAAGGCAAATATTCAACTCAATGTCACCAAGAGCTAG
Protein sequence

>MsaT003893.1

MAFSRNGIVIPNQDDYADIEGEDDDELEEEDNYFVQPNIKNYQTEQSLSTFTTTATIPTTIILALPNTAATTNIIDDSLEPKWEQMEEKNNDSQKLFQCLWTKEDEITILQGLLDYNANRGSSYHNDTGSFYDQIKANIQLNVTKS*