AlfalfaGEDB | MsaG003945

M. sativa ssp. caerulea / MsaG003945

KEGG: K08066
GO: GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0003712 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009889 GO:0009987 GO:0010029 GO:0010033 GO:0010099 GO:0010228 GO:0010468 GO:0010476 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044444 GO:0044464 GO:0048518 GO:0048519 GO:0048571 GO:0048573 GO:0048574 GO:0048579 GO:0048580 GO:0048581 GO:0048582 GO:0048583 GO:0048584 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051094 GO:0051171 GO:0051239 GO:0051240 GO:0051241 GO:0051252 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0080090 GO:0097159 GO:0097305 GO:0097306 GO:0140110 GO:1900140 GO:1901363 GO:1901700 GO:1901701 GO:1903506 GO:2000026 GO:2000028 GO:2000030 GO:2000112 GO:2000241 GO:2000242 GO:2000306 GO:2001141
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003945	XP_003591084.1	98.828	256	2	1	1	255	1	256	0.0	518

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003945	sp\|Q8L4B2\|NFYC9_ARATH	64.344	244	60	9	1	242	1	219	1.70e-85	257

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003945	tr\|B7FI76\|B7FI76_MEDTR	98.828	256	2	1	1	255	1	256	0.0	518

TFs/TRs:

Gene ID	Type	Classification
MsaG003945	TF	NF-YC

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000125	MsaG003945	0.817762	4.854243e-52	1.006977e-49
MsaG003945	MsaG004047	0.812639	6.589514e-51	1.200583e-48
MsaG003945	MsaG004419	0.828693	1.396998e-54	3.895221e-52
MsaG003945	MsaG006023	0.814329	2.812016e-51	5.344799e-49
MsaG003945	MsaG006034	0.814486	2.596821e-51	4.955290e-49
MsaG003945	MsaG006203	0.820349	1.260291e-52	2.797656e-50
MsaG003945	MsaG006267	0.823355	2.558644e-53	6.154661e-51
MsaG003945	MsaG006783	0.803191	6.598417e-49	9.593194e-47
MsaG003945	MsaG007328	0.820010	1.505379e-52	3.311827e-50
MsaG003945	MsaG007614	0.837704	8.151705e-57	2.962592e-54
MsaG003945	MsaG008109	0.864130	3.020922e-64	2.756773e-61
MsaG003945	MsaG011974	0.875949	4.275810e-68	6.414289e-65
MsaG003945	MsaG012644	0.817367	5.952084e-52	1.222251e-49
MsaG003945	MsaG015023	0.816463	9.478707e-52	1.901602e-49
MsaG003945	MsaG017095	0.841925	6.566244e-58	2.724218e-55
MsaG003945	MsaG018020	0.876966	1.912335e-68	3.002850e-65
MsaG003945	MsaG018701	0.808292	5.664440e-50	9.282999e-48
MsaG003945	MsaG018744	0.835769	2.525892e-56	8.657600e-54
MsaG003945	MsaG020143	0.811774	1.015741e-50	1.811466e-48
MsaG003945	MsaG021043	0.801377	1.552809e-48	2.165795e-46
MsaG003945	MsaG023331	0.819728	1.745352e-52	3.810807e-50
MsaG003945	MsaG023618	0.802979	7.296077e-49	1.055550e-46
MsaG003945	MsaG023719	0.807342	9.000048e-50	1.441681e-47
MsaG003945	MsaG023992	0.835599	2.787586e-56	9.506401e-54
MsaG003945	MsaG024094	0.805337	2.369552e-49	3.620616e-47
MsaG003945	MsaG025969	0.819311	2.169877e-52	4.686554e-50
MsaG003945	MsaG028062	0.829213	1.046830e-54	2.961814e-52
MsaG003945	MsaG029385	0.808654	4.746489e-50	7.846814e-48
MsaG003945	MsaG032479	0.816590	8.877168e-52	1.786791e-49
MsaG003945	MsaG032477	0.813823	3.631717e-51	6.816048e-49
MsaG003945	MsaG034394	0.828289	1.746645e-54	4.815271e-52
MsaG003945	MsaG038393	0.813581	4.104825e-51	7.657025e-49
MsaG003945	MsaG042475	0.809223	3.589466e-50	6.015661e-48
MsaG003945	MsaG045301	0.833022	1.226260e-55	3.873450e-53
MsaG003945	MsaG045291	0.811745	1.030434e-50	1.836365e-48
MsaG003945	MsaG045598	0.822683	3.663876e-53	8.654064e-51
MsaG003945	MsaG046544	0.838594	4.824289e-57	1.802287e-54
MsaG003945	MsaG047049	0.846785	3.291252e-59	1.600115e-56
MsaG003945	MsaG001485	0.806662	1.251262e-49	1.972438e-47
MsaG003945	MsaG014699	0.808416	5.331594e-50	8.763505e-48
MsaG003945	MsaG032753	0.803839	4.850583e-49	7.158302e-47
MsaG003945	MsaG033344	0.848475	1.134018e-59	5.834325e-57
MsaG003945	MsaG037135	0.837455	9.436898e-57	3.403941e-54

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003945	MtrunA17_Chr1g0191681	98.828	256	2	1	1	255	1	256	0.0	518
MsaG003945	MtrunA17_Chr7g0272991	81.154	260	44	2	1	255	1	260	8.29e-145	405
MsaG003945	MtrunA17_Chr2g0310741	58.065	217	75	8	16	226	20	226	1.33e-69	215
MsaG003945	MtrunA17_Chr3g0132191	82.524	103	18	0	83	185	37	139	1.84e-58	185
MsaG003945	MtrunA17_Chr2g0288481	50.000	184	70	7	82	254	66	238	2.27e-53	172
MsaG003945	MtrunA17_Chr2g0318881	59.341	91	36	1	100	189	21	111	8.17e-33	116
MsaG003945	MtrunA17_Chr2g0318921	63.855	83	29	1	100	181	21	103	3.29e-32	114
MsaG003945	MtrunA17_Chr8g0376351	61.333	75	27	1	119	191	1	75	2.48e-27	100
MsaG003945	MtrunA17_Chr3g0081161	39.744	78	47	0	102	179	9	86	4.26e-12	64.7
MsaG003945	MtrunA17_Chr8g0354151	37.179	78	49	0	102	179	9	86	1.08e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003945	AT1G08970.3	64.344	244	60	9	1	242	1	219	1.73e-86	257
MsaG003945	AT1G08970.4	64.344	244	60	9	1	242	1	219	1.73e-86	257
MsaG003945	AT1G08970.2	64.344	244	60	9	1	242	1	219	1.73e-86	257
MsaG003945	AT1G08970.1	64.344	244	60	9	1	242	1	219	1.73e-86	257
MsaG003945	AT1G54830.2	64.655	232	56	4	23	254	12	217	2.81e-86	256
MsaG003945	AT1G54830.3	64.655	232	56	4	23	254	12	217	2.81e-86	256
MsaG003945	AT1G54830.1	64.655	232	56	4	23	254	12	217	2.81e-86	256
MsaG003945	AT1G56170.1	67.785	149	34	3	80	226	55	191	1.76e-66	205
MsaG003945	AT1G56170.2	67.785	149	34	3	80	226	55	191	1.76e-66	205
MsaG003945	AT3G48590.1	69.863	146	41	3	83	228	47	189	3.11e-64	201
MsaG003945	AT5G63470.2	68.456	149	41	3	83	228	60	205	1.71e-61	194
MsaG003945	AT5G63470.1	68.456	149	41	3	83	228	60	205	1.71e-61	194
MsaG003945	AT5G50480.1	48.276	174	55	7	79	243	35	182	6.37e-44	147
MsaG003945	AT5G27910.1	47.794	136	61	3	78	206	16	148	1.15e-35	125
MsaG003945	AT5G50490.1	39.106	179	90	6	71	244	9	173	3.10e-32	117
MsaG003945	AT5G50470.1	48.148	108	47	2	79	180	44	148	1.20e-28	108
MsaG003945	AT5G38140.1	45.000	100	51	2	80	178	48	144	5.12e-26	101

Find 88 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTGATATCTTTGATTTCT+TGG	0.152930	1:+64103080	MsaT003945.1:CDS
CTACCAACAACACCCATATT+TGG	0.174332	1:-64102610	None:intergenic
TGGAGTTAACCTTGCGTTCT+TGG	0.214555	1:+64102992	MsaT003945.1:CDS
TCATGGCCAAAACCCAAATA+TGG	0.254115	1:+64102597	MsaT003945.1:CDS
AACAACAACTCCAGACCTTT+TGG	0.267438	1:+64102812	MsaT003945.1:CDS
CAACACCCATATTTGGGTTT+TGG	0.289322	1:-64102603	None:intergenic
GTCATTGGGTTTGGCTGGTA+TGG	0.344732	1:-64102661	None:intergenic
TACCAACAACACCCATATTT+GGG	0.353196	1:-64102609	None:intergenic
ATTGTTGTTGCTCCGGTGGT+TGG	0.362576	1:-64103311	None:intergenic
TTGTTGTTGCTCCGGTGGTT+GGG	0.366842	1:-64103310	None:intergenic
GGTGACCCAGTCATTGGGTT+TGG	0.375542	1:-64102670	None:intergenic
GGGATGGTTGTTCCCTCTGT+TGG	0.377579	1:+64102694	MsaT003945.1:CDS
GCACAGCAAGCTGGAAATCC+TGG	0.386872	1:+64103219	MsaT003945.1:CDS
GGATGGTTGTTCCCTCTGTT+GGG	0.395560	1:+64102695	MsaT003945.1:CDS
ATCCCAGGTGACCCAGTCAT+TGG	0.407383	1:-64102676	None:intergenic
TGAGGCGGCATATAGCAATA+AGG	0.408347	1:-64103192	None:intergenic
ACCCAGTCATTGGGTTTGGC+TGG	0.417557	1:-64102666	None:intergenic
AACCCAATGACTGGGTCACC+TGG	0.422707	1:+64102673	MsaT003945.1:CDS
GGTGGTTGGGGCCACATTTG+AGG	0.430701	1:-64103297	None:intergenic
CACGCCCTTGCAAATACAAC+CGG	0.432250	1:-64102955	None:intergenic
CTTGGTTGACATTGTGCCTA+GGG	0.441263	1:+64103098	MsaT003945.1:CDS
AACCCAAATATGGGTGTTGT+TGG	0.445950	1:+64102607	MsaT003945.1:CDS
ACTGATTTCCCCAAAAGGTC+TGG	0.448617	1:-64102822	None:intergenic
TCCCAGGTGACCCAGTCATT+GGG	0.450412	1:-64102675	None:intergenic
CACATTTGAGGTGCCATGTA+TGG	0.456179	1:-64103285	None:intergenic
TCAAATGTGGCCCCAACCAC+CGG	0.464006	1:+64103299	MsaT003945.1:CDS
AGGCGCCGGTTGTATTTGCA+AGG	0.466746	1:+64102950	MsaT003945.1:CDS
TGTTGTTGGTAGTGGACAAA+TGG	0.476420	1:+64102621	MsaT003945.1:CDS
TGTTGTTGCTCCGGTGGTTG+GGG	0.477662	1:-64103309	None:intergenic
GTTCCATCAGGTTGACCACC+AGG	0.484332	1:-64102727	None:intergenic
TCAACCTGATGGAACTCAGC+TGG	0.487041	1:+64102735	MsaT003945.1:CDS
CCAGCAGGCATCGGTCCTCT+TGG	0.488412	1:-64103150	None:intergenic
TTGATACTGATTTCCCCAAA+AGG	0.488519	1:-64102827	None:intergenic
CCAAGAGGACCGATGCCTGC+TGG	0.496911	1:+64103150	MsaT003945.1:CDS
TGTCCCAGCTGAGTTCCATC+AGG	0.498006	1:-64102739	None:intergenic
TCTTGGTTGACATTGTGCCT+AGG	0.499762	1:+64103097	MsaT003945.1:CDS
CCTCAAAATGCACAGCAAGC+TGG	0.503997	1:+64103210	MsaT003945.1:CDS
ATCACACTGAAGAGAACAAA+AGG	0.504661	1:+64103016	MsaT003945.1:CDS
TACCAGCCAAACCCAATGAC+TGG	0.504830	1:+64102664	MsaT003945.1:CDS
GACTGTGGAGCATACATGTT+GGG	0.509025	1:-64103258	None:intergenic
GGCGTGTGAAATGTTCATAT+TGG	0.517805	1:+64102972	MsaT003945.1:CDS
CCATCAGCAGGTCCTCCAGC+AGG	0.525310	1:-64103165	None:intergenic
TGGTATGGATTAGAACCGTA+TGG	0.527698	1:-64102646	None:intergenic
TCTTGATCCTTGCCAAGGGA+AGG	0.527734	1:-64102885	None:intergenic
AATATGGGTGTTGTTGGTAG+TGG	0.529665	1:+64102613	MsaT003945.1:CDS
TCATCTTTCAAGTCCTCCCT+AGG	0.530171	1:-64103114	None:intergenic
CATGGCCAAAACCCAAATAT+GGG	0.534350	1:+64102598	MsaT003945.1:CDS
GGATTATGCATCATGAGTCC+AGG	0.538672	1:-64103237	None:intergenic
CAACCTGATGGAACTCAGCT+GGG	0.543463	1:+64102736	MsaT003945.1:CDS
CAATAAGGAATTCCATCAGC+AGG	0.543484	1:-64103177	None:intergenic
GGCGATTGTTGTTGCTCCGG+TGG	0.545817	1:-64103315	None:intergenic
AATCTTCTTGATCCTTGCCA+AGG	0.551207	1:-64102890	None:intergenic
GATGATATCAGCTGAGGCGC+CGG	0.551962	1:+64102936	MsaT003945.1:CDS
CCTGCTGGAGGACCTGCTGA+TGG	0.553104	1:+64103165	MsaT003945.1:CDS
CAACCTGGTGGTCAACCTGA+TGG	0.553542	1:+64102724	MsaT003945.1:CDS
GAAGGCGATTGTTGTTGCTC+CGG	0.554014	1:-64103318	None:intergenic
ATAAGAATCTAATGATCTGA+AGG	0.560767	1:-64103336	None:intergenic
GACATTGCAGCAGCAATCAC+AGG	0.562745	1:+64103057	MsaT003945.1:CDS
GAAGATTATGAAGGCTGATG+AGG	0.566353	1:+64102906	MsaT003945.1:CDS
ACCCAATGACTGGGTCACCT+GGG	0.574510	1:+64102674	MsaT003945.1:CDS
GCAGGTCCTCCAGCAGGCAT+CGG	0.575223	1:-64103159	None:intergenic
GGTATGGATTAGAACCGTAT+GGG	0.576207	1:-64102645	None:intergenic
GGTTGACATTGTGCCTAGGG+AGG	0.582771	1:+64103101	MsaT003945.1:CDS
ATCTTCTTGATCCTTGCCAA+GGG	0.584713	1:-64102889	None:intergenic
TCCACAGTCTCATCCATACA+TGG	0.588194	1:+64103272	MsaT003945.1:CDS
TCCTTGCCAAGGGAAGGCTG+TGG	0.591000	1:-64102879	None:intergenic
ACCACAGCCTTCCCTTGGCA+AGG	0.592871	1:+64102878	MsaT003945.1:CDS
GGCGCCGGTTGTATTTGCAA+GGG	0.593275	1:+64102951	MsaT003945.1:CDS
ACAGAGGGAACAACCATCCC+AGG	0.593577	1:-64102691	None:intergenic
AAGGATCAAGAAGATTATGA+AGG	0.595150	1:+64102897	MsaT003945.1:CDS
AGTGGACAAATGGCCCCATA+CGG	0.596575	1:+64102631	MsaT003945.1:CDS
AGAATGGATCATCAAGGTCA+TGG	0.604516	1:+64102580	None:intergenic
AATGACTGGGTCACCTGGGA+TGG	0.607597	1:+64102678	MsaT003945.1:CDS
TGAAACAGAATGGATCATCA+AGG	0.611963	1:+64102574	None:intergenic
TCTGTTGGGACTATTCAACC+TGG	0.614722	1:+64102709	MsaT003945.1:CDS
CATACATGGCACCTCAAATG+TGG	0.616231	1:+64103286	MsaT003945.1:CDS
ACCAGCCAAACCCAATGACT+GGG	0.624633	1:+64102665	MsaT003945.1:CDS
AGAGGACCGATGCCTGCTGG+AGG	0.625695	1:+64103153	MsaT003945.1:CDS
AGTGTGATTCCAAGAACGCA+AGG	0.629484	1:-64103001	None:intergenic
GGTTGAATAGTCCCAACAGA+GGG	0.660370	1:-64102706	None:intergenic
TCAGTATCAAGAAATCGAGA+AGG	0.663994	1:+64102840	MsaT003945.1:CDS
TGTGAAGATGATATCAGCTG+AGG	0.675937	1:+64102930	MsaT003945.1:CDS
GAAGTGCTTGCATTACCAAG+AGG	0.676504	1:+64103135	MsaT003945.1:CDS
GTTGGGACTATTCAACCTGG+TGG	0.686021	1:+64102712	MsaT003945.1:CDS
AGACTGTGGAGCATACATGT+TGG	0.689302	1:-64103259	None:intergenic
GTATGGATTAGAACCGTATG+GGG	0.698616	1:-64102644	None:intergenic
GCCATGTATGGATGAGACTG+TGG	0.701999	1:-64103273	None:intergenic
AGGTTGAATAGTCCCAACAG+AGG	0.742829	1:-64102707	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	64102583	64103350	64102583	ID=MsaG003945
Chr1	mRNA	64102583	64103350	64102583	ID=MsaT003945.1;Parent=MsaG003945
Chr1	exon	64102583	64103350	64102583	ID=MsaT003945.1.exon1;Parent=MsaT003945.1
Chr1	CDS	64102583	64103350	64102583	ID=cds.MsaT003945.1;Parent=MsaT003945.1

Gene Sequence

>MsaT003945.1

ATGGATCATCAAGGTCATGGCCAAAACCCAAATATGGGTGTTGTTGGTAGTGGACAAATGGCCCCATACGGTTCTAATCCATACCAGCCAAACCCAATGACTGGGTCACCTGGGATGGTTGTTCCCTCTGTTGGGACTATTCAACCTGGTGGTCAACCTGATGGAACTCAGCTGGGACAACATCAGCTTGCTTATCAGCACATTCATCAGCAGCAACAGCAGCAGCAACAACAACAACTCCAGACCTTTTGGGGAAATCAGTATCAAGAAATCGAGAAGGTAACTGATTTCAAAAACCACAGCCTTCCCTTGGCAAGGATCAAGAAGATTATGAAGGCTGATGAGGATGTGAAGATGATATCAGCTGAGGCGCCGGTTGTATTTGCAAGGGCGTGTGAAATGTTCATATTGGAGTTAACCTTGCGTTCTTGGAATCACACTGAAGAGAACAAAAGGCGAACCCTTCAAAAGAATGACATTGCAGCAGCAATCACAGGAACTGATATCTTTGATTTCTTGGTTGACATTGTGCCTAGGGAGGACTTGAAAGATGAAGTGCTTGCATTACCAAGAGGACCGATGCCTGCTGGAGGACCTGCTGATGGAATTCCTTATTGCTATATGCCGCCTCAAAATGCACAGCAAGCTGGAAATCCTGGACTCATGATGCATAATCCCAACATGTATGCTCCACAGTCTCATCCATACATGGCACCTCAAATGTGGCCCCAACCACCGGAGCAACAACAATCGCCTTCAGATCATTAG

Protein sequence

>MsaT003945.1

MDHQGHGQNPNMGVVGSGQMAPYGSNPYQPNPMTGSPGMVVPSVGTIQPGGQPDGTQLGQHQLAYQHIHQQQQQQQQQQLQTFWGNQYQEIEKVTDFKNHSLPLARIKKIMKADEDVKMISAEAPVVFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAAITGTDIFDFLVDIVPREDLKDEVLALPRGPMPAGGPADGIPYCYMPPQNAQQAGNPGLMMHNPNMYAPQSHPYMAPQMWPQPPEQQQSPSDH*