AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004028


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004028 MtrunA17_Chr1g0192791 97.388 268 6 1 1 267 1 268 0.0 543
MsaG004028 MtrunA17_Chr5g0413201 53.696 257 103 4 9 259 63 309 9.71e-85 256
MsaG004028 MtrunA17_Chr1g0151961 48.413 252 117 4 18 264 119 362 8.26e-70 219
MsaG004028 MtrunA17_Chr5g0439391 48.594 249 103 4 19 259 47 278 2.23e-67 211
MsaG004028 MtrunA17_Chr2g0289201 50.206 243 107 4 18 256 117 349 2.53e-65 208
MsaG004028 MtrunA17_Chr1g0149651 34.538 249 138 6 19 244 174 420 1.76e-37 137
MsaG004028 MtrunA17_Chr3g0137241 35.249 261 122 8 19 244 107 355 1.80e-37 135
MsaG004028 MtrunA17_Chr5g0398181 33.597 253 145 4 15 244 24 276 1.28e-36 132
MsaG004028 MtrunA17_Chr4g0065241 33.065 248 147 4 16 244 68 315 2.39e-36 132
MsaG004028 MtrunA17_Chr2g0305101 34.274 248 138 6 19 244 95 339 3.00e-35 129
MsaG004028 MtrunA17_Chr6g0479181 34.906 106 67 1 15 120 64 167 8.43e-13 65.5
MsaG004028 MtrunA17_Chr7g0240371 38.356 73 43 1 174 246 296 366 4.84e-11 62.8
MsaG004028 MtrunA17_Chr4g0008591 42.466 73 39 2 174 246 352 421 5.78e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004028 AT1G23380.1 51.190 252 103 4 17 261 84 322 3.26e-83 253
MsaG004028 AT1G70510.1 50.602 249 109 3 17 261 68 306 1.21e-81 248
MsaG004028 AT1G23380.2 50.787 254 103 5 17 261 84 324 3.95e-81 247
MsaG004028 AT1G70510.2 49.802 253 109 4 17 261 68 310 2.75e-79 242
MsaG004028 AT1G62360.1 49.798 247 108 4 18 258 121 357 2.99e-67 213
MsaG004028 AT4G08150.1 47.177 248 117 5 18 258 134 374 5.31e-66 211
MsaG004028 AT1G62990.1 34.661 251 142 6 15 244 26 275 1.65e-39 139
MsaG004028 AT5G25220.2 35.200 250 132 6 19 244 160 403 2.11e-38 139
MsaG004028 AT5G25220.1 35.200 250 132 6 19 244 160 403 2.14e-38 139
MsaG004028 AT5G11060.1 35.156 256 124 8 19 244 124 367 1.80e-37 136
MsaG004028 AT4G32040.2 34.146 246 140 5 19 244 119 362 9.55e-37 134
MsaG004028 AT4G32040.1 34.146 246 140 5 19 244 119 362 1.02e-36 134
MsaG004028 AT1G75430.1 49.091 55 28 0 192 246 209 263 5.73e-11 62.0
MsaG004028 AT5G02030.1 46.667 60 32 0 187 246 350 409 8.92e-11 62.4

Find 61 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTCTCTTCTTGAAGAAAT+AGG 0.173162 1:-64900794 MsaT004028.1:CDS
GAAATGCTCCTTCGTAAGTA+TGG 0.209222 1:-64896020 MsaT004028.1:CDS
TCTCACGGAAAGCATTATTT+TGG 0.227729 1:+64900759 None:intergenic
ATTTCTCAAGAAAAGGAAAA+AGG 0.267954 1:-64895970 MsaT004028.1:CDS
CTTCTCTTCTTGAAGAAATA+GGG 0.308161 1:-64900793 MsaT004028.1:CDS
ACAACCAATCGGATCTAATA+AGG 0.325364 1:-64901148 MsaT004028.1:CDS
ATATAAAGCAGATAAATAAT+TGG 0.326164 1:-64895301 MsaT004028.1:CDS
TTTACCTGAACGGTTGTAAT+CGG 0.376328 1:+64900411 None:intergenic
TGTTGCTTCATTGAAGGGCT+TGG 0.378477 1:+64900498 None:intergenic
AATAATGCTTTCCGTGAGAT+AGG 0.392343 1:-64900755 MsaT004028.1:CDS
GATAGTAGATTTGGATAAAG+AGG 0.394577 1:+64901080 None:intergenic
TCCTGACCTTGACCACTTCA+TGG 0.397547 1:-64900726 MsaT004028.1:intron
AAATAATGTTGCTTCATTGA+AGG 0.405931 1:+64900492 None:intergenic
GACAGGAATTTCTCAAGAAA+AGG 0.410633 1:-64895977 MsaT004028.1:CDS
ATTTGAATTACCGTGGGATA+AGG 0.413318 1:+64895885 None:intergenic
AATAATGTTGCTTCATTGAA+GGG 0.423656 1:+64900493 None:intergenic
GGATGCAAGAAAAGCATTGA+TGG 0.424199 1:-64895934 MsaT004028.1:CDS
GGAAAGGTTGAAGCAGTAGA+AGG 0.448898 1:-64896095 MsaT004028.1:CDS
ATTGGTGGAACGTCCACTAT+AGG 0.452627 1:-64895911 MsaT004028.1:CDS
TCATCCGATTACAACCGTTC+AGG 0.455239 1:-64900415 MsaT004028.1:intron
GGGTCAGATCACGAGGCAGC+AGG 0.458108 1:-64896143 MsaT004028.1:intron
TTACAGAGGTCCTGCTATGC+TGG 0.477108 1:+64895202 None:intergenic
ACCATGAAGTGGTCAAGGTC+AGG 0.484448 1:+64900725 None:intergenic
TGGGATAAGGCCACCTATAG+TGG 0.485346 1:+64895898 None:intergenic
AAATGCTCCTTCGTAAGTAT+GGG 0.496084 1:-64896019 MsaT004028.1:CDS
ATTCCTGTCTTAAGTTGCTA+AGG 0.503658 1:+64895991 None:intergenic
CAAGAAAAGCATTGATGGAT+TGG 0.506407 1:-64895929 MsaT004028.1:CDS
AAAGAGGGTGAGTTGCGATC+TGG 0.510035 1:+64901096 None:intergenic
GTAAGTAGCACCAGCATAGC+AGG 0.513299 1:-64895212 MsaT004028.1:CDS
TCTAATAAGGTTGGATATGA+CGG 0.529171 1:-64901135 MsaT004028.1:CDS
AGAAGAGAAGCAAGTTCTGT+TGG 0.544757 1:+64900806 None:intergenic
ATAGTAGATTTGGATAAAGA+GGG 0.546531 1:+64901081 None:intergenic
CCAATCGGATCTAATAAGGT+TGG 0.547810 1:-64901144 MsaT004028.1:CDS
TCTTTAAGCTCTTTATCACC+TGG 0.551142 1:+64896041 None:intergenic
CCAACCTTATTAGATCCGAT+TGG 0.554336 1:+64901144 None:intergenic
GAAAAGCATTGATGGATTGG+TGG 0.555034 1:-64895926 MsaT004028.1:CDS
TTACCTTAGCAACTTAAGAC+AGG 0.555596 1:-64895994 MsaT004028.1:CDS
ACAACTAAACGAACTTTGCA+AGG 0.560050 1:-64900452 MsaT004028.1:CDS
TCACATGGAACACAACCAAT+CGG 0.562048 1:-64901159 None:intergenic
CTGCAGCTGTCAGATATGAC+TGG 0.564508 1:-64895329 MsaT004028.1:CDS
TTAAGTTCTCTTTACCTGAA+CGG 0.565840 1:+64900401 None:intergenic
TCAGAGGATGAAATGAGTTG+TGG 0.568316 1:-64896116 MsaT004028.1:CDS
AATATACCATGAAGTGGTCA+AGG 0.574865 1:+64900720 None:intergenic
TAAAAGAATATACCATGAAG+TGG 0.580028 1:+64900714 None:intergenic
TCAATCAGAGGAAAAGACAC+TGG 0.584588 1:-64895274 MsaT004028.1:CDS
CAGGATCATCTCCTATCTCA+CGG 0.597806 1:+64900744 None:intergenic
CTTTGTGGCACATCATGTCC+AGG 0.602003 1:-64896059 MsaT004028.1:CDS
GGTGGTCATGATGAACTTTG+TGG 0.607949 1:-64896074 MsaT004028.1:CDS
AATGCTCCTTCGTAAGTATG+GGG 0.615416 1:-64896018 MsaT004028.1:CDS
CTATAGGTGGCCTTATCCCA+CGG 0.616947 1:-64895895 MsaT004028.1:intron
CAACTAAACGAACTTTGCAA+GGG 0.626350 1:-64900451 MsaT004028.1:CDS
AACTAAACGAACTTTGCAAG+GGG 0.629556 1:-64900450 MsaT004028.1:CDS
GGTCAGATCACGAGGCAGCA+GGG 0.632522 1:-64896142 MsaT004028.1:intron
ATAATTGGTTCATCAATCAG+AGG 0.632866 1:-64895286 MsaT004028.1:CDS
CGAGGCAGCAGGGACATCAG+AGG 0.640139 1:-64896132 MsaT004028.1:CDS
AGTTCTTCATAGATACAAAG+AGG 0.649236 1:-64900527 MsaT004028.1:CDS
GGTGGAACGTCCACTATAGG+TGG 0.655633 1:-64895908 MsaT004028.1:CDS
GGATGAAATGAGTTGTGGAA+AGG 0.661174 1:-64896111 MsaT004028.1:CDS
AAGGTTGAAGCAGTAGAAGG+TGG 0.681013 1:-64896092 MsaT004028.1:CDS
ATGCTCCTTCGTAAGTATGG+GGG 0.702025 1:-64896017 MsaT004028.1:CDS
CCTGCAGAATAAATTTACAG+AGG 0.707916 1:+64895188 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 64895202 64901177 64895202 ID=MsaG004028
Chr1 mRNA 64895202 64901177 64895202 ID=MsaT004028.1;Parent=MsaG004028
Chr1 exon 64895202 64895363 64895202 ID=MsaT004028.1.exon5;Parent=MsaT004028.1
Chr1 CDS 64895202 64895363 64895202 ID=cds.MsaT004028.1;Parent=MsaT004028.1
Chr1 exon 64895896 64896158 64895896 ID=MsaT004028.1.exon4;Parent=MsaT004028.1
Chr1 CDS 64895896 64896158 64895896 ID=cds.MsaT004028.1;Parent=MsaT004028.1
Chr1 exon 64900416 64900563 64900416 ID=MsaT004028.1.exon3;Parent=MsaT004028.1
Chr1 CDS 64900416 64900563 64900416 ID=cds.MsaT004028.1;Parent=MsaT004028.1
Chr1 exon 64900727 64900837 64900727 ID=MsaT004028.1.exon2;Parent=MsaT004028.1
Chr1 CDS 64900727 64900837 64900727 ID=cds.MsaT004028.1;Parent=MsaT004028.1
Chr1 exon 64901058 64901177 64901058 ID=MsaT004028.1.exon1;Parent=MsaT004028.1
Chr1 CDS 64901058 64901177 64901058 ID=cds.MsaT004028.1;Parent=MsaT004028.1
Gene Sequence

>MsaT004028.1

ATGGAACACAACCAATCGGATCTAATAAGGTTGGATATGACGGATCGAATTATCAAGAACCAGATCGCAACTCACCCTCTTTATCCAAATCTACTATCTGCTTTTTTAGAATGCCAAAAGGTTGGGGCACCAACAGAACTTGCTTCTCTTCTTGAAGAAATAGGGCGTGAAAACCACCAAAATAATGCTTTCCGTGAGATAGGAGATGATCCTGACCTTGACCACTTCATGGAATCATACTGTGAAGTTCTTCATAGATACAAAGAGGAGTTATCCAAGCCCTTCAATGAAGCAACATTATTTTTGTGCAATATTGAATCACAACTAAACGAACTTTGCAAGGGGACACAAACAATGTCATCCGATTACAACCGTTCAGATCACGAGGCAGCAGGGACATCAGAGGATGAAATGAGTTGTGGAAAGGTTGAAGCAGTAGAAGGTGGTCATGATGAACTTTGTGGCACATCATGTCCAGGTGATAAAGAGCTTAAAGAAATGCTCCTTCGTAAGTATGGGGGTTACCTTAGCAACTTAAGACAGGAATTTCTCAAGAAAAGGAAAAAGGGTAAACTACCAAAGGATGCAAGAAAAGCATTGATGGATTGGTGGAACGTCCACTATAGGTGGCCTTATCCCACGGAGGAGGAGAAACTGCAGCTGTCAGATATGACTGGACTTGATATAAAGCAGATAAATAATTGGTTCATCAATCAGAGGAAAAGACACTGGAAACCATCTGAAAATATGAGATTTTCTATTATGGAGGGTGTAAGTAGCACCAGCATAGCAGGACCTCTGTAA

Protein sequence

>MsaT004028.1

MEHNQSDLIRLDMTDRIIKNQIATHPLYPNLLSAFLECQKVGAPTELASLLEEIGRENHQNNAFREIGDDPDLDHFMESYCEVLHRYKEELSKPFNEATLFLCNIESQLNELCKGTQTMSSDYNRSDHEAAGTSEDEMSCGKVEAVEGGHDELCGTSCPGDKELKEMLLRKYGGYLSNLRQEFLKKRKKGKLPKDARKALMDWWNVHYRWPYPTEEEKLQLSDMTGLDIKQINNWFINQRKRHWKPSENMRFSIMEGVSSTSIAGPL*