AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004058


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004058 MtrunA17_Chr1g0193071 86.719 128 11 2 1 128 1 122 1.76e-70 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004058 AT5G26749.1 40.136 147 69 2 1 128 1 147 5.47e-32 110
MsaG004058 AT5G26749.2 35.542 166 69 3 1 128 1 166 1.87e-27 99.8

Find 44 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCTGGCATTCTTGTTAT+TGG 0.166998 1:+65261673 None:intergenic
TGCTGCTGCTGTTGTGTATT+GGG 0.205632 1:+65261697 None:intergenic
TACCGGCAGAGTGGCGTTTC+CGG 0.248184 1:+65261958 None:intergenic
AGAGTGGCGTTTCCGGTCTA+AGG 0.283984 1:+65261965 None:intergenic
TTGCTGCTGCTGTTGTGTAT+TGG 0.317673 1:+65261696 None:intergenic
GGAATAGAGTTCTGGTGTTC+GGG 0.348282 1:+65261891 None:intergenic
TGGAATAGAGTTCTGGTGTT+CGG 0.377509 1:+65261890 None:intergenic
ATCTATCCCCAGTCGACAAA+TGG 0.397656 1:+65261462 None:intergenic
ATGGTGGTGGAATAGAGTTC+TGG 0.430967 1:+65261883 None:intergenic
GGAATCGTACCAACGAGCTT+TGG 0.434321 1:+65261920 None:intergenic
CAACCTCCCATTTGTCGACT+GGG 0.457123 1:-65261469 MsaT004058.1:CDS
GTCACTGCAGCCTCCGCCTG+AGG 0.458331 1:-65261502 MsaT004058.1:CDS
TCACTGCAGCCTCCGCCTGA+GGG 0.463350 1:-65261501 MsaT004058.1:CDS
GTTCTGGTGTTCGGGTTTGA+AGG 0.464430 1:+65261899 None:intergenic
ACGCAGAAGGGTTGATTAGA+TGG 0.466779 1:+65261864 None:intergenic
GGCTTGTTTGTGTTGAATAC+CGG 0.496651 1:+65261941 None:intergenic
TCTATCCCCAGTCGACAAAT+GGG 0.506048 1:+65261463 None:intergenic
GATACCATGGGAGTGTCGTT+CGG 0.508317 1:-65261537 MsaT004058.1:CDS
GACCGGAAACGCCACTCTGC+CGG 0.512557 1:-65261960 MsaT004058.1:CDS
CCAACCTCCCATTTGTCGAC+TGG 0.514767 1:-65261470 MsaT004058.1:CDS
TCAGGCGGAGGCTGCAGTGA+CGG 0.518687 1:+65261504 None:intergenic
CTGCAGCCTCCGCCTGAGGG+TGG 0.519587 1:-65261498 MsaT004058.1:CDS
AAGGGTTGATTAGATGGTGG+TGG 0.521880 1:+65261870 None:intergenic
TTCAAGATACACCCTTAGAC+CGG 0.529755 1:-65261977 MsaT004058.1:CDS
GAGTATCCACCCTCAGGCGG+AGG 0.540027 1:+65261492 None:intergenic
AGGTTGGAGTATCCACCCTC+AGG 0.543831 1:+65261486 None:intergenic
ATATATGTAGGGAATATAGT+AGG 0.551592 1:-65261561 MsaT004058.1:intron
CCAGTCGACAAATGGGAGGT+TGG 0.561835 1:+65261470 None:intergenic
ATAGTACCGTGTTGACGAAG+TGG 0.572113 1:+65261841 None:intergenic
CAACCAATAACAAGAATGCC+AGG 0.578455 1:-65261676 MsaT004058.1:intron
GAGTGGCGTTTCCGGTCTAA+GGG 0.584406 1:+65261966 None:intergenic
TTGACGAAGTGGACGCAGAA+GGG 0.593699 1:+65261852 None:intergenic
GTTGACGAAGTGGACGCAGA+AGG 0.597332 1:+65261851 None:intergenic
GGCGGAGGCTGCAGTGACGG+AGG 0.613435 1:+65261507 None:intergenic
GAATATAGTAGGAGATACCA+TGG 0.619077 1:-65261550 MsaT004058.1:CDS
CTGCGTCCACTTCGTCAACA+CGG 0.619782 1:-65261847 MsaT004058.1:intron
ACAAACAAGCCAAAGCTCGT+TGG 0.650557 1:-65261929 MsaT004058.1:CDS
ATTGCCGAACGACACTCCCA+TGG 0.650589 1:+65261533 None:intergenic
CAGAAGGGTTGATTAGATGG+TGG 0.659317 1:+65261867 None:intergenic
TTGGAGTATCCACCCTCAGG+CGG 0.670531 1:+65261489 None:intergenic
ATCCCCAGTCGACAAATGGG+AGG 0.671698 1:+65261466 None:intergenic
AACCTCCCATTTGTCGACTG+GGG 0.672189 1:-65261468 MsaT004058.1:CDS
AATATAGTAGGAGATACCAT+GGG 0.697591 1:-65261549 MsaT004058.1:CDS
TGTGTTGAATACCGGCAGAG+TGG 0.711189 1:+65261949 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 65261464 65262042 65261464 ID=MsaG004058
Chr1 mRNA 65261464 65262042 65261464 ID=MsaT004058.1;Parent=MsaG004058
Chr1 exon 65261464 65261573 65261464 ID=MsaT004058.1.exon3;Parent=MsaT004058.1
Chr1 CDS 65261464 65261573 65261464 ID=cds.MsaT004058.1;Parent=MsaT004058.1
Chr1 exon 65261677 65261758 65261677 ID=MsaT004058.1.exon2;Parent=MsaT004058.1
Chr1 CDS 65261677 65261758 65261677 ID=cds.MsaT004058.1;Parent=MsaT004058.1
Chr1 exon 65261848 65262042 65261848 ID=MsaT004058.1.exon1;Parent=MsaT004058.1
Chr1 CDS 65261848 65262042 65261848 ID=cds.MsaT004058.1;Parent=MsaT004058.1
Gene Sequence

>MsaT004058.1

ATGCCAATGAAAAAGTACTATTGTGAGTATTGCGATAAGCAATTTCAAGATACACCCTTAGACCGGAAACGCCACTCTGCCGGTATTCAACACAAACAAGCCAAAGCTCGTTGGTACGATTCCTTCAAACCCGAACACCAGAACTCTATTCCACCACCATCTAATCAACCCTTCTGCGTCCACTTCGTCAACACGGGATTTTGTCGTTATGGCGATTCTTGCAAATATTTTCATCCCAATACACAACAGCAGCAGCAACCAATAACAAGAATGCCAGGGAATATAGTAGGAGATACCATGGGAGTGTCGTTCGGCAATTTGCCTCCGTCACTGCAGCCTCCGCCTGAGGGTGGATACTCCAACCTCCCATTTGTCGACTGGGGATAG

Protein sequence

>MsaT004058.1

MPMKKYYCEYCDKQFQDTPLDRKRHSAGIQHKQAKARWYDSFKPEHQNSIPPPSNQPFCVHFVNTGFCRYGDSCKYFHPNTQQQQQPITRMPGNIVGDTMGVSFGNLPPSLQPPPEGGYSNLPFVDWG*