AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004093

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004093 MtrunA17_Chr1g0193321 96.949 721 20 1 1 719 1 721 0.0 1439
MsaG004093 MtrunA17_Chr7g0271251 48.714 739 285 17 1 708 2 677 0.0 597
MsaG004093 MtrunA17_Chr1g0182791 61.333 150 54 2 395 544 254 399 1.59e-43 165
MsaG004093 MtrunA17_Chr7g0262691 55.000 160 53 5 396 542 185 338 6.36e-37 144
MsaG004093 MtrunA17_Chr3g0095391 61.654 133 39 5 425 557 276 396 1.27e-36 144
MsaG004093 MtrunA17_Chr7g0270871 50.314 159 71 3 407 561 152 306 1.26e-33 132
MsaG004093 MtrunA17_Chr5g0402031 50.336 149 60 2 402 549 59 194 7.40e-28 115
MsaG004093 MtrunA17_Chr1g0153241 65.882 85 29 0 454 538 79 163 1.72e-27 112
MsaG004093 MtrunA17_Chr7g0231461 55.435 92 40 1 432 522 268 359 6.69e-23 102
MsaG004093 MtrunA17_Chr4g0074161 55.405 74 29 1 462 531 208 281 1.04e-18 89.4
MsaG004093 MtrunA17_Chr1g0190731 52.857 70 29 1 462 527 195 264 1.40e-16 82.0
MsaG004093 MtrunA17_Chr1g0152131 47.945 73 37 1 462 533 262 334 1.64e-15 79.7
MsaG004093 MtrunA17_Chr5g0393721 35.294 119 61 3 444 561 207 310 2.31e-15 78.2
MsaG004093 MtrunA17_Chr8g0363921 50.000 64 31 1 462 524 153 216 1.49e-14 74.7
MsaG004093 MtrunA17_Chr3g0144641 45.205 73 39 1 462 533 193 265 2.42e-14 75.9
MsaG004093 MtrunA17_Chr8g0365941 42.105 95 47 3 462 548 307 401 3.91e-14 75.5
MsaG004093 MtrunA17_Chr5g0421111 46.575 73 38 1 462 533 367 439 4.35e-14 75.9
MsaG004093 MtrunA17_Chr1g0207311 36.538 104 46 3 463 561 194 282 4.81e-14 73.6
MsaG004093 MtrunA17_Chr7g0234761 51.562 64 30 1 462 524 319 382 5.12e-14 75.5
MsaG004093 MtrunA17_Chr5g0415291 38.583 127 63 5 409 524 55 177 5.24e-14 72.4
MsaG004093 MtrunA17_Chr4g0035101 45.205 73 39 1 462 533 145 217 5.86e-14 73.9
MsaG004093 MtrunA17_Chr8g0339091 51.562 64 30 1 462 524 293 356 7.97e-14 74.7
MsaG004093 MtrunA17_Chr3g0131771 50.000 64 31 1 462 524 149 212 2.96e-13 71.6
MsaG004093 MtrunA17_Chr3g0134741 36.275 102 50 3 458 559 227 313 4.32e-13 70.9
MsaG004093 MtrunA17_Chr8g0385911 40.000 80 47 1 449 527 134 213 8.34e-13 70.1
MsaG004093 MtrunA17_Chr1g0207301 36.047 86 40 2 476 561 304 374 2.31e-12 69.3
MsaG004093 MtrunA17_Chr7g0258161 43.056 72 40 1 463 533 176 247 2.90e-12 68.6
MsaG004093 MtrunA17_Chr1g0152651 45.455 66 36 0 458 523 272 337 3.34e-12 68.9
MsaG004093 MtrunA17_Chr1g0174161 41.429 70 40 1 463 531 163 232 1.61e-11 66.2
MsaG004093 MtrunA17_Chr1g0184841 50.000 74 33 1 462 531 122 195 6.81e-11 63.9
MsaG004093 MtrunA17_Chr6g0484271 45.312 64 34 1 462 524 157 220 7.41e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004093 AT1G09530.3 35.703 619 221 24 1 595 1 466 1.50e-71 242
MsaG004093 AT1G09530.2 35.703 619 221 24 1 595 1 466 1.50e-71 242
MsaG004093 AT1G09530.5 35.703 619 221 24 1 595 1 466 1.50e-71 242
MsaG004093 AT1G09530.1 35.703 619 221 24 1 595 1 466 1.50e-71 242
MsaG004093 AT1G09530.4 35.703 619 221 24 1 595 1 466 1.50e-71 242
MsaG004093 AT1G09530.6 35.703 619 221 24 1 595 1 466 1.50e-71 242
MsaG004093 AT2G20180.7 47.458 177 72 5 399 572 153 311 1.51e-34 137
MsaG004093 AT2G20180.1 47.458 177 72 5 399 572 153 311 1.51e-34 137
MsaG004093 AT2G20180.5 47.458 177 72 5 399 572 153 311 1.51e-34 137
MsaG004093 AT2G46970.2 46.193 197 78 7 395 576 127 310 1.58e-34 136
MsaG004093 AT2G46970.1 46.193 197 78 7 395 576 158 341 1.96e-34 136
MsaG004093 AT2G46970.3 46.193 197 78 7 395 576 136 319 2.61e-34 135
MsaG004093 AT2G20180.4 47.458 177 72 5 399 572 224 382 6.59e-34 136
MsaG004093 AT2G20180.6 47.458 177 72 5 399 572 224 382 6.59e-34 136
MsaG004093 AT2G20180.2 47.458 177 72 5 399 572 224 382 6.59e-34 136
MsaG004093 AT2G20180.3 47.458 177 72 5 399 572 224 382 6.59e-34 136
MsaG004093 AT2G20180.8 52.381 126 47 3 399 521 224 339 2.41e-29 120
MsaG004093 AT4G28800.1 43.827 162 67 5 392 548 193 335 1.80e-27 116
MsaG004093 AT4G28790.1 43.709 151 65 4 421 564 244 381 1.01e-26 114
MsaG004093 AT4G36930.1 58.772 114 41 2 432 541 161 272 1.40e-26 112
MsaG004093 AT4G00050.2 59.140 93 34 2 432 523 79 168 3.01e-26 110
MsaG004093 AT4G00050.3 59.140 93 34 2 432 523 181 270 1.26e-25 110
MsaG004093 AT4G00050.1 59.140 93 34 2 432 523 181 270 1.43e-25 110
MsaG004093 AT5G67110.1 54.639 97 44 0 440 536 67 163 1.86e-24 102
MsaG004093 AT3G59060.1 47.552 143 60 3 425 555 212 351 3.05e-23 103
MsaG004093 AT3G59060.3 47.552 143 60 3 425 555 212 351 3.60e-23 103
MsaG004093 AT3G59060.2 47.552 143 60 3 425 555 212 351 3.60e-23 103
MsaG004093 AT3G59060.4 47.552 143 60 3 425 555 212 351 3.60e-23 103
MsaG004093 AT3G62090.1 46.341 123 48 2 460 573 165 278 5.57e-23 101
MsaG004093 AT4G28800.2 41.358 162 65 6 392 548 205 341 5.59e-23 103
MsaG004093 AT3G62090.2 46.341 123 48 2 460 573 182 295 1.15e-22 100
MsaG004093 AT4G28815.2 52.222 90 40 2 456 542 64 153 3.30e-22 96.7
MsaG004093 AT4G28815.1 48.958 96 46 2 450 542 134 229 1.83e-21 96.3
MsaG004093 AT2G43010.2 66.667 81 24 1 445 522 233 313 1.19e-20 95.9
MsaG004093 AT2G43010.1 66.667 81 24 1 445 522 233 313 1.21e-20 95.9
MsaG004093 AT2G43010.4 66.667 81 24 1 445 522 233 313 1.28e-20 95.5
MsaG004093 AT2G43010.3 66.667 81 24 1 445 522 240 320 1.41e-20 95.5
MsaG004093 AT2G43010.5 66.667 81 24 1 445 522 275 355 2.16e-20 95.5
MsaG004093 AT4G28790.2 48.000 100 40 2 421 520 244 331 8.43e-20 92.0
MsaG004093 AT2G24260.2 60.317 63 25 0 462 524 37 99 1.81e-19 89.0
MsaG004093 AT5G58010.1 53.947 76 31 1 462 533 101 176 3.59e-19 89.4
MsaG004093 AT2G24260.1 60.317 63 25 0 462 524 140 202 7.13e-19 89.4
MsaG004093 AT4G30980.1 58.730 63 26 0 462 524 132 194 1.40e-18 87.8
MsaG004093 AT5G61270.2 48.246 114 48 3 423 536 45 147 3.10e-18 86.3
MsaG004093 AT5G61270.3 48.246 114 48 3 423 536 45 147 3.10e-18 86.3
MsaG004093 AT4G30980.2 58.730 63 26 0 462 524 180 242 3.55e-18 87.4
MsaG004093 AT5G61270.1 48.246 114 48 3 423 536 133 235 5.54e-18 87.0
MsaG004093 AT4G28811.1 41.916 167 72 5 392 545 292 446 8.95e-17 84.7
MsaG004093 AT1G68920.1 31.795 195 103 6 357 533 195 377 2.54e-15 79.7
MsaG004093 AT1G68920.4 31.795 195 103 6 357 533 195 377 2.54e-15 79.7
MsaG004093 AT1G68920.2 31.795 195 103 6 357 533 195 377 2.54e-15 79.7
MsaG004093 AT1G68920.3 49.315 73 36 1 462 533 304 376 2.94e-15 79.7
MsaG004093 AT5G50915.3 53.125 64 29 1 462 524 138 201 3.67e-15 76.6
MsaG004093 AT5G50915.4 53.125 64 29 1 462 524 138 201 3.67e-15 76.6
MsaG004093 AT1G10120.2 47.945 73 37 1 462 533 208 280 4.53e-15 78.2
MsaG004093 AT1G10120.1 47.945 73 37 1 462 533 208 280 4.53e-15 78.2
MsaG004093 AT5G50915.1 53.125 64 29 1 462 524 138 201 5.19e-15 76.6
MsaG004093 AT5G50915.2 53.125 64 29 1 462 524 138 201 5.19e-15 76.6
MsaG004093 AT1G02340.2 47.727 88 44 2 474 559 134 221 8.95e-15 75.9
MsaG004093 AT1G02340.1 47.727 88 44 2 474 559 141 228 9.65e-15 75.9
MsaG004093 AT2G18300.1 45.333 75 40 1 462 535 187 261 2.02e-14 75.5
MsaG004093 AT2G18300.2 45.333 75 40 1 462 535 187 261 2.15e-14 75.5
MsaG004093 AT3G23690.1 44.156 77 42 1 462 537 193 269 2.28e-14 75.9
MsaG004093 AT4G36540.2 45.205 73 39 1 462 533 143 215 2.76e-14 74.7
MsaG004093 AT2G18300.3 44.156 77 42 1 462 537 187 263 2.82e-14 75.5
MsaG004093 AT4G36540.1 45.205 73 39 1 462 533 143 215 2.91e-14 74.7
MsaG004093 AT5G62610.2 51.562 64 30 1 462 524 155 218 4.23e-14 73.9
MsaG004093 AT5G62610.1 51.562 64 30 1 462 524 155 218 4.23e-14 73.9
MsaG004093 AT1G59640.1 50.000 64 31 1 462 524 138 201 5.60e-14 73.2
MsaG004093 AT2G14760.1 38.462 104 49 3 458 561 235 323 5.84e-14 74.3
MsaG004093 AT3G07340.2 51.562 64 30 1 462 524 260 323 6.35e-14 75.1
MsaG004093 AT2G14760.3 37.931 116 51 4 452 561 236 336 7.25e-14 73.9
MsaG004093 AT3G07340.1 51.562 64 30 1 462 524 260 323 7.42e-14 75.1
MsaG004093 AT5G48560.2 51.562 64 30 1 462 524 303 366 1.04e-13 74.7
MsaG004093 AT5G48560.1 51.562 64 30 1 462 524 303 366 1.23e-13 74.3
MsaG004093 AT4G33880.1 40.000 100 45 3 458 557 264 348 1.32e-13 73.6
MsaG004093 AT4G34530.1 41.667 72 41 1 462 532 174 245 1.74e-13 72.8
MsaG004093 AT5G43175.1 41.250 80 39 2 451 530 130 201 2.79e-13 70.5
MsaG004093 AT1G26260.5 48.438 64 32 1 462 524 203 266 2.93e-13 72.4
MsaG004093 AT1G26260.3 48.438 64 32 1 462 524 174 237 2.94e-13 72.0
MsaG004093 AT2G42300.4 43.836 73 40 1 462 533 187 259 3.20e-13 71.2
MsaG004093 AT1G26260.4 48.438 64 32 1 462 524 225 288 3.40e-13 72.4
MsaG004093 AT1G26260.1 48.438 64 32 1 462 524 225 288 3.40e-13 72.4
MsaG004093 AT1G26260.6 48.438 64 32 1 462 524 225 288 3.40e-13 72.4
MsaG004093 AT1G26260.2 48.438 64 32 1 462 524 225 288 3.40e-13 72.4
MsaG004093 AT1G59640.2 50.000 64 31 1 462 524 138 201 3.65e-13 72.0
MsaG004093 AT1G27740.1 36.066 122 58 5 440 561 152 253 5.35e-13 70.1
MsaG004093 AT2G42300.1 43.836 73 40 1 462 533 187 259 5.98e-13 71.2
MsaG004093 AT3G57800.2 42.466 73 41 1 462 533 206 278 1.83e-12 70.1
MsaG004093 AT1G18400.2 46.875 64 33 1 462 524 147 210 2.27e-12 68.2
MsaG004093 AT1G18400.1 46.875 64 33 1 462 524 147 210 2.66e-12 68.2
MsaG004093 AT1G25330.1 45.312 64 34 1 462 524 106 169 4.15e-12 67.0
MsaG004093 AT1G73830.2 43.750 64 35 1 462 524 149 212 1.09e-11 66.2
MsaG004093 AT1G73830.1 43.750 64 35 1 462 524 149 212 1.23e-11 66.2

Find 192 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAATGGGAATCAGATATTT+AGG 0.194680 1:+65598010 MsaT004093.1:CDS
CAACCTTCCATTCAAGTTCT+TGG 0.207186 1:+65598675 MsaT004093.1:CDS
TCACCAAACTTTCCTATCTT+TGG 0.207484 1:-65597802 None:intergenic
TGAATCAGTGGGAGTTAAAA+AGG 0.229978 1:+65598958 MsaT004093.1:CDS
TACTCAAGGGTTTGGACTTC+CGG 0.238298 1:+65600050 MsaT004093.1:CDS
GGACACACAGCTGCTGAAAA+AGG 0.246020 1:+65598714 MsaT004093.1:CDS
TTCAGCAGCACGGCTTCTTT+TGG 0.263273 1:-65599008 None:intergenic
TAGCTGCAGGCCTTGCAAAA+TGG 0.265088 1:-65598353 None:intergenic
GAACCATTGCCAGGCATTAT+AGG 0.273673 1:-65598312 None:intergenic
GGCTCTTTGCAACACGAATA+TGG 0.299730 1:+65597913 MsaT004093.1:CDS
ACCTTATGACAGCGCATTTC+CGG 0.308325 1:-65598537 None:intergenic
AAACTAACCTTATTGCAATT+AGG 0.311300 1:-65599214 None:intergenic
GCATTTGGTTCAAGCAATTT+CGG 0.321748 1:-65598597 None:intergenic
ATTATTAACTTACTTCACTA+TGG 0.325057 1:-65598854 None:intergenic
AGAACCGATGAATGTTTCTA+AGG 0.325663 1:+65598053 MsaT004093.1:CDS
TTCCGTTTCAAATCTCGATT+TGG 0.325821 1:-65598804 None:intergenic
CATCTGTAACTGTTAGATCT+AGG 0.327984 1:+65598166 MsaT004093.1:CDS
GGTAACCAGGACGTGCCTTA+TGG 0.332319 1:+65598225 MsaT004093.1:CDS
AGCTGCAGGCCTTGCAAAAT+GGG 0.334857 1:-65598352 None:intergenic
AGGGATAAACAGACCTGGTT+AGG 0.337445 1:-65600291 None:intergenic
ATAAGAGGCCCTCCTGAAAA+AGG 0.349955 1:-65600114 None:intergenic
ACTCAAGGGTTTGGACTTCC+GGG 0.350946 1:+65600051 MsaT004093.1:CDS
AATCCAAAGATAGGAAAGTT+TGG 0.360484 1:+65597799 MsaT004093.1:CDS
TAAGGGTTCTTCAGCTGAAC+AGG 0.362772 1:+65598071 MsaT004093.1:CDS
GCCTCTTCTTCTGTTTGCTC+TGG 0.374903 1:+65598753 MsaT004093.1:CDS
AGTAATAACAATCCAAAGAT+AGG 0.377772 1:+65597790 MsaT004093.1:CDS
TCTTTATGAGTTATACCGTT+TGG 0.379797 1:+65597407 MsaT004093.1:CDS
ATGGGGTTAGGTATGGGTTA+TGG 0.384832 1:+65599895 MsaT004093.1:CDS
ATCTGAGGTACTTGAATCAT+TGG 0.390961 1:-65599958 None:intergenic
GTAACCAGGACGTGCCTTAT+GGG 0.391349 1:+65598226 MsaT004093.1:CDS
CATGCCAATGCCGCATGGTC+CGG 0.391369 1:+65600086 MsaT004093.1:CDS
CATGCATATGGGGTTAGGTA+TGG 0.391760 1:+65599888 MsaT004093.1:CDS
TTAATTTGTGAGTCCACATC+TGG 0.393466 1:-65600231 None:intergenic
ACTCATACCTAATTGCAATA+AGG 0.396247 1:+65599207 MsaT004093.1:CDS
GATGGGCATCGGCATGCATA+TGG 0.396774 1:+65599876 MsaT004093.1:CDS
TGTTTAGTAGCTACAGCATT+TGG 0.397325 1:-65598612 None:intergenic
GTTATAGGAGTTAAGAATAA+AGG 0.400952 1:+65598444 MsaT004093.1:CDS
TGACAGTGAAGCTGCTAATC+CGG 0.402149 1:+65600495 MsaT004093.1:CDS
GCGGCTGGACGAGGAGTTGC+AGG 0.404515 1:+65598983 MsaT004093.1:CDS
TCCTTTGAACACTCACCGCT+TGG 0.414948 1:-65598513 None:intergenic
CAGTCAACTTTGGTGCATAA+TGG 0.417435 1:+65600451 MsaT004093.1:intron
AGATCGAATACTCAAGGGTT+TGG 0.418683 1:+65600042 MsaT004093.1:CDS
TCAGCAAGATCAGATGTTAT+AGG 0.425064 1:+65598429 MsaT004093.1:CDS
AACAGACCTGGTTAGGGATC+TGG 0.426475 1:-65600284 None:intergenic
GAATGATATTTACTTGAACT+TGG 0.428430 1:-65599683 None:intergenic
CGGTGAGTGTTCAAAGGAAC+CGG 0.431291 1:+65598518 MsaT004093.1:CDS
TACCTCTACTTGATTGACCT+TGG 0.431493 1:-65597678 None:intergenic
GCTCCGGGTCCGTCTGTGTA+TGG 0.432008 1:+65600147 MsaT004093.1:CDS
TCTTCTGTTTGCTCTGGCAA+TGG 0.432421 1:+65598759 MsaT004093.1:CDS
TTTGAGCTGGTTTGGGAGAG+TGG 0.433011 1:+65597643 MsaT004093.1:CDS
GGTCCAGGCATATGCGCAAC+GGG 0.433702 1:-65599997 None:intergenic
AGGGTTTGGACTTCCGGGAC+AGG 0.436904 1:+65600056 MsaT004093.1:CDS
AATGCCGGACCATGCGGCAT+TGG 0.437560 1:-65600090 None:intergenic
GACGGCCCGCCGTTCATGTC+AGG 0.437600 1:-65599928 None:intergenic
CAGAACATAGGCTTGAAGTC+TGG 0.440984 1:+65598393 MsaT004093.1:CDS
TCGCGAGATGGCACCGACAT+TGG 0.449982 1:-65597850 None:intergenic
ATGCCCGTTGCGCATATGCC+TGG 0.457121 1:+65599994 MsaT004093.1:CDS
ACCGGAAATGCGCTGTCATA+AGG 0.458097 1:+65598536 MsaT004093.1:CDS
GGATTAACAAGAGCTGAATC+AGG 0.461722 1:-65598492 None:intergenic
TTAGGTATGGGTTATGGAAT+GGG 0.462375 1:+65599901 MsaT004093.1:CDS
AAGTTTGGTGATTTAGACTC+TGG 0.465005 1:+65597814 MsaT004093.1:CDS
ATTACATTGGAACCATTGCC+AGG 0.465626 1:-65598321 None:intergenic
TGGATTTAAACAGTCAACTT+TGG 0.465882 1:+65600441 MsaT004093.1:intron
TATTGGATAAAAGAAGTAAT+GGG 0.466226 1:+65597995 MsaT004093.1:CDS
ATGGGCATCGGCATGCATAT+GGG 0.467830 1:+65599877 MsaT004093.1:CDS
GTAGAAAGAGTCCATCTTGT+AGG 0.469763 1:+65597698 MsaT004093.1:CDS
ACATGTTGAAGCTGTGAATC+CGG 0.471250 1:+65600179 MsaT004093.1:CDS
GCCTTGGTTGGATTATTCAA+TGG 0.473067 1:+65597888 MsaT004093.1:CDS
ATTCCATTCTCTTTGCAAGC+AGG 0.474924 1:-65598642 None:intergenic
CTTGGTGACAACGGCGCTAA+AGG 0.476187 1:+65598693 MsaT004093.1:CDS
TAATGGGAATCAGATATTTA+GGG 0.478431 1:+65598011 MsaT004093.1:CDS
TCTTATGAACTCATCCGCTC+CGG 0.478517 1:+65600131 MsaT004093.1:CDS
AATCACAAATTTATATTGCC+CGG 0.480981 1:-65600514 None:intergenic
TGGTTGGATTATTCAATGGA+TGG 0.481215 1:+65597892 MsaT004093.1:CDS
CAAAGGTTTCAAATCAGCCT+TGG 0.482518 1:-65598571 None:intergenic
CCAGCAGGTAACATCATTTG+AGG 0.491436 1:-65599811 None:intergenic
ATGCATATGGGGTTAGGTAT+GGG 0.501740 1:+65599889 MsaT004093.1:CDS
ATCCAAATCGAGATTTGAAA+CGG 0.502394 1:+65598802 MsaT004093.1:CDS
TTTGAGCCACACGCTGAAGC+CGG 0.503540 1:-65600198 None:intergenic
CTATTGGATAAAAGAAGTAA+TGG 0.505191 1:+65597994 MsaT004093.1:CDS
CAAATTAAGCAAAACACTGC+TGG 0.508355 1:+65600246 MsaT004093.1:CDS
GACAGTGAAGCTGCTAATCC+GGG 0.512717 1:+65600496 MsaT004093.1:CDS
AAGCCTGCTTGCAAAGAGAA+TGG 0.512967 1:+65598639 MsaT004093.1:CDS
GTTTGACATTGATTCGATGA+TGG 0.513871 1:-65598141 None:intergenic
ATCGGCATGCATATGGGGTT+AGG 0.513942 1:+65599883 MsaT004093.1:CDS
CAATAGAGTAAAACTATTCG+AGG 0.514401 1:-65597977 None:intergenic
CTTATGAACTCATCCGCTCC+GGG 0.516950 1:+65600132 MsaT004093.1:CDS
CCTCAAATGATGTTACCTGC+TGG 0.519335 1:+65599811 MsaT004093.1:CDS
ATCTGTAACTGTTAGATCTA+GGG 0.521505 1:+65598167 MsaT004093.1:CDS
GGTGGATAAAGCTTCTATGC+TGG 0.522318 1:+65599626 MsaT004093.1:intron
GTTGTCACCAAGAACTTGAA+TGG 0.522686 1:-65598682 None:intergenic
TCAAGCAATTTCGGCTGCAA+AGG 0.523909 1:-65598588 None:intergenic
TGATTTCTTTCATGAACTCA+CGG 0.530210 1:+65597939 MsaT004093.1:CDS
AAACGGTATAACTCATAAAG+AGG 0.530629 1:-65597405 None:intergenic
GGAGCGGATGAGTTCATAAG+AGG 0.531123 1:-65600129 None:intergenic
GGCATGGAAATTCCCTGTCC+CGG 0.531882 1:-65600069 None:intergenic
GGGTTTGGACTTCCGGGACA+GGG 0.531895 1:+65600057 MsaT004093.1:CDS
TCTTACGCAGATGATGTCAA+TGG 0.532008 1:+65599771 MsaT004093.1:intron
GGGATAAACAGACCTGGTTA+GGG 0.536075 1:-65600290 None:intergenic
GTTAGGTATGGGTTATGGAA+TGG 0.536992 1:+65599900 MsaT004093.1:CDS
GCCAGAGCAAACAGAAGAAG+AGG 0.539271 1:-65598754 None:intergenic
AAGCAAGATCCTATAATGCC+TGG 0.540589 1:+65598303 MsaT004093.1:CDS
TGCATGTGCTGCATTCCAGC+AGG 0.541526 1:-65599826 None:intergenic
GTATACAGAGGCGAAGAGAC+AGG 0.541533 1:+65599155 MsaT004093.1:intron
TTAGCTCTCACAATAGCTGC+AGG 0.542020 1:-65598366 None:intergenic
AGAGTAAAACTATTCGAGGC+AGG 0.545061 1:-65597973 None:intergenic
GTGAATCCGGCTTCAGCGTG+TGG 0.545306 1:+65600192 MsaT004093.1:CDS
AGATGAAATGATGCCTTGGT+TGG 0.547275 1:+65597876 MsaT004093.1:CDS
ACTTTAATTGAAGGATGTTG+AGG 0.547453 1:+65598934 MsaT004093.1:intron
CCGTCTGTGTATGGATCCAC+AGG 0.548020 1:+65600156 MsaT004093.1:CDS
GGTTTCAAATCAGCCTTGGA+TGG 0.548035 1:-65598567 None:intergenic
ACGTCTTTATCTCGACCTCT+AGG 0.548572 1:-65597754 None:intergenic
TATCCCCATAAGGCACGTCC+TGG 0.548638 1:-65598230 None:intergenic
TAATGTTAGCAGTGGTAACC+AGG 0.548677 1:+65598212 MsaT004093.1:CDS
AAACCCTTGAGTATTCGATC+TGG 0.548724 1:-65600040 None:intergenic
AGTGCATAATCTATCTGAAA+GGG 0.548993 1:+65599030 MsaT004093.1:CDS
GCACATGCATGCACCACACA+TGG 0.551028 1:+65599840 MsaT004093.1:CDS
GACTAACGGCGCAAGTGTAG+CGG 0.551051 1:+65597452 MsaT004093.1:CDS
CGGACCATGCGGCATTGGCA+TGG 0.552943 1:-65600085 None:intergenic
CCTGGACCCTCTGCTATGCA+TGG 0.553478 1:+65600012 MsaT004093.1:CDS
GAATGCCTGACATGAACGGC+GGG 0.554517 1:+65599923 MsaT004093.1:CDS
CGAGATAAAGACGTAGGAGG+AGG 0.554717 1:+65597763 MsaT004093.1:CDS
CTTACGCAGATGATGTCAAT+GGG 0.556698 1:+65599772 MsaT004093.1:intron
ATTTCATCTACTTCGCGAGA+TGG 0.557156 1:-65597862 None:intergenic
ATGCGAGTAATATCCCCATA+AGG 0.560313 1:-65598240 None:intergenic
CAAGCTTCTCTCTAGCCAAA+CGG 0.561344 1:-65597422 None:intergenic
TCACCAAGAACTTGAATGGA+AGG 0.562453 1:-65598678 None:intergenic
GAACCGATGAATGTTTCTAA+GGG 0.563235 1:+65598054 MsaT004093.1:CDS
AGAGCTAATCTTCAGAACAT+AGG 0.563549 1:+65598381 MsaT004093.1:CDS
TAATGGTGGCCATGCTTCAG+TGG 0.563671 1:+65600468 MsaT004093.1:CDS
TAAAGCTTCTATGCTGGATG+AGG 0.564004 1:+65599632 MsaT004093.1:CDS
TACAAGCCAGATCCCTAACC+AGG 0.569454 1:+65600278 MsaT004093.1:CDS
CAGATGATGTCAATGGGAGC+TGG 0.570696 1:+65599778 MsaT004093.1:intron
GGGTCCAGGCATATGCGCAA+CGG 0.570830 1:-65599998 None:intergenic
ATATGATTTCCTTGCATCTG+AGG 0.571553 1:-65599973 None:intergenic
CCATGCATAGCAGAGGGTCC+AGG 0.572604 1:-65600012 None:intergenic
ATGGCCAGATCGAATACTCA+AGG 0.580647 1:+65600036 MsaT004093.1:CDS
TCCAAGCGGTGAGTGTTCAA+AGG 0.581477 1:+65598512 MsaT004093.1:CDS
GAAAATAATAATGTTAGCAG+TGG 0.582796 1:+65598204 MsaT004093.1:CDS
ACCCTCTGCTATGCATGGAA+TGG 0.583329 1:+65600017 MsaT004093.1:CDS
TGGGCATCGGCATGCATATG+GGG 0.587722 1:+65599878 MsaT004093.1:CDS
AAAAGATCGAAGGCCAGATG+TGG 0.594060 1:+65600218 MsaT004093.1:CDS
GATCCTATAATGCCTGGCAA+TGG 0.595775 1:+65598309 MsaT004093.1:CDS
AAGTGCATAATCTATCTGAA+AGG 0.598272 1:+65599029 MsaT004093.1:CDS
ATTCAAGTTCTTGGTGACAA+CGG 0.601682 1:+65598684 MsaT004093.1:CDS
GCAAGACATAAAGCTAGTGC+CGG 0.602436 1:+65598105 MsaT004093.1:CDS
GATTATGCACTTCAGCAGCA+CGG 0.604449 1:-65599018 None:intergenic
CAATGGTGCAGACAGAGGCT+CGG 0.605241 1:+65598776 MsaT004093.1:CDS
TGAGATGGTAAGCAGTGAGA+TGG 0.605275 1:-65597727 None:intergenic
CACAGCTTCAACATGTCCTG+TGG 0.605340 1:-65600172 None:intergenic
GCCATTCCATGCATAGCAGA+GGG 0.607653 1:-65600018 None:intergenic
AGCCAAGGTCAATCAAGTAG+AGG 0.608356 1:+65597676 MsaT004093.1:CDS
GATCCATACACAGACGGACC+CGG 0.610764 1:-65600150 None:intergenic
GATGGCAAACTCCTACAAGA+TGG 0.610890 1:-65597709 None:intergenic
TCTGGCAATGGTGCAGACAG+AGG 0.613703 1:+65598771 MsaT004093.1:CDS
TAACCAGGACGTGCCTTATG+GGG 0.616802 1:+65598227 MsaT004093.1:CDS
TGTTAATGAACTTACGACGA+AGG 0.616840 1:-65597480 None:intergenic
ATTTCCATGCCAATGCCGCA+TGG 0.619250 1:+65600081 MsaT004093.1:CDS
TGTCATCAGCCACTGAAGCA+TGG 0.619461 1:-65600477 None:intergenic
ATTCAAGTACCTCAGATGCA+AGG 0.620716 1:+65599964 MsaT004093.1:CDS
GAACCCTTAGAAACATTCAT+CGG 0.621432 1:-65598057 None:intergenic
TAAAACTATTCGAGGCAGGA+AGG 0.623215 1:-65597969 None:intergenic
GGCCATTCCATGCATAGCAG+AGG 0.624817 1:-65600019 None:intergenic
GATGTTGAGGATGAATCAGT+GGG 0.625050 1:+65598947 MsaT004093.1:CDS
TCAGCTCTTGTTAATCCAAG+CGG 0.627460 1:+65598498 MsaT004093.1:CDS
TGATGGCGGATATAACTGAC+CGG 0.631000 1:-65598124 None:intergenic
GGTCGAGATAAAGACGTAGG+AGG 0.631029 1:+65597760 MsaT004093.1:CDS
TGAATCATTGGAAATCTAGA+CGG 0.631570 1:-65599946 None:intergenic
AAGCTTGATAATGAGACTAA+CGG 0.634639 1:+65597438 MsaT004093.1:CDS
CGTGTGGCTCAAAAGATCGA+AGG 0.637154 1:+65600208 MsaT004093.1:CDS
TCAACTTTGGTGCATAATGG+TGG 0.640234 1:+65600454 MsaT004093.1:CDS
GGATGTTGAGGATGAATCAG+TGG 0.640619 1:+65598946 MsaT004093.1:intron
AAAGGAAATGCCGGACCATG+CGG 0.646962 1:-65600096 None:intergenic
TTACCATCTCATAGTCCTAG+AGG 0.648723 1:+65597739 MsaT004093.1:CDS
CGACCTCTAGGACTATGAGA+TGG 0.648898 1:-65597742 None:intergenic
AGTGGTCAGATTTCAAGCCA+AGG 0.648899 1:+65597661 MsaT004093.1:CDS
ATGGGAATGCCTGACATGAA+CGG 0.649844 1:+65599919 MsaT004093.1:CDS
GGAAATGCGCTGTCATAAGG+TGG 0.651300 1:+65598539 MsaT004093.1:CDS
AAGTAGATGAAATGATGCCT+TGG 0.653879 1:+65597872 MsaT004093.1:CDS
TCCATTGAATAATCCAACCA+AGG 0.654964 1:-65597889 None:intergenic
TGACATTGATTCGATGATGG+CGG 0.655078 1:-65598138 None:intergenic
TAAGGTGGTAGAGCCATCCA+AGG 0.655378 1:+65598554 MsaT004093.1:CDS
TGTGAAAATGCGGCCATGTG+TGG 0.658564 1:-65599853 None:intergenic
GGAATGCCTGACATGAACGG+CGG 0.663426 1:+65599922 MsaT004093.1:CDS
TGGCCAGATCGAATACTCAA+GGG 0.668407 1:+65600037 MsaT004093.1:CDS
AGAGGTCGAGATAAAGACGT+AGG 0.676780 1:+65597757 MsaT004093.1:CDS
CCTGTGGATCCATACACAGA+CGG 0.678896 1:-65600156 None:intergenic
TTAACAAGAGCTGAATCAGG+AGG 0.682638 1:-65598489 None:intergenic
ATTGAATGAGATTCCAATGT+CGG 0.685130 1:+65597837 MsaT004093.1:CDS
GATGCCCATCTGTGAAAATG+CGG 0.689000 1:-65599863 None:intergenic
ATACACAGACGGACCCGGAG+CGG 0.699444 1:-65600145 None:intergenic
ACGGAAAAGTAGAGACACTG+AGG 0.744015 1:+65598821 MsaT004093.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 65597402 65600534 65597402 ID=MsaG004093
Chr1 mRNA 65597402 65600534 65597402 ID=MsaT004093.1;Parent=MsaG004093
Chr1 exon 65597402 65597487 65597402 ID=MsaT004093.1.exon1;Parent=MsaT004093.1
Chr1 CDS 65597402 65597487 65597402 ID=cds.MsaT004093.1;Parent=MsaT004093.1
Chr1 exon 65597627 65598863 65597627 ID=MsaT004093.1.exon2;Parent=MsaT004093.1
Chr1 CDS 65597627 65598863 65597627 ID=cds.MsaT004093.1;Parent=MsaT004093.1
Chr1 exon 65598947 65599051 65598947 ID=MsaT004093.1.exon3;Parent=MsaT004093.1
Chr1 CDS 65598947 65599051 65598947 ID=cds.MsaT004093.1;Parent=MsaT004093.1
Chr1 exon 65599163 65599228 65599163 ID=MsaT004093.1.exon4;Parent=MsaT004093.1
Chr1 CDS 65599163 65599228 65599163 ID=cds.MsaT004093.1;Parent=MsaT004093.1
Chr1 exon 65599627 65599692 65599627 ID=MsaT004093.1.exon5;Parent=MsaT004093.1
Chr1 CDS 65599627 65599692 65599627 ID=cds.MsaT004093.1;Parent=MsaT004093.1
Chr1 exon 65599781 65600299 65599781 ID=MsaT004093.1.exon6;Parent=MsaT004093.1
Chr1 CDS 65599781 65600299 65599781 ID=cds.MsaT004093.1;Parent=MsaT004093.1
Chr1 exon 65600454 65600534 65600454 ID=MsaT004093.1.exon7;Parent=MsaT004093.1
Chr1 CDS 65600454 65600534 65600454 ID=cds.MsaT004093.1;Parent=MsaT004093.1
Gene Sequence

>MsaT004093.1

ATGCCTCTTTATGAGTTATACCGTTTGGCTAGAGAGAAGCTTGATAATGAGACTAACGGCGCAAGTGTAGCGGATCAACCTTCGTCGCCTGAGAATGATTTTTTTGAGCTGGTTTGGGAGAGTGGTCAGATTTCAAGCCAAGGTCAATCAAGTAGAGGTAGAAAGAGTCCATCTTGTAGGAGTTTGCCATCTCACTGCTTACCATCTCATAGTCCTAGAGGTCGAGATAAAGACGTAGGAGGAGGATTTAGTAATAACAATCCAAAGATAGGAAAGTTTGGTGATTTAGACTCTGGATTGAATGAGATTCCAATGTCGGTGCCATCTCGCGAAGTAGATGAAATGATGCCTTGGTTGGATTATTCAATGGATGGCTCTTTGCAACACGAATATGGTTCTGATTTCTTTCATGAACTCACGGAGAACGACCTTCCTGCCTCGAATAGTTTTACTCTATTGGATAAAAGAAGTAATGGGAATCAGATATTTAGGGACTCTCATAAAAATTCTGCAGAACCGATGAATGTTTCTAAGGGTTCTTCAGCTGAACAGGTTGTTGAGACTGCAAGACATAAAGCTAGTGCCGGTCAGTTATATCCGCCATCATCGAATCAATGTCAAACAACATCTGTAACTGTTAGATCTAGGGCATCAGATATAACTGAAAATAATAATGTTAGCAGTGGTAACCAGGACGTGCCTTATGGGGATATTACTCGCATTCCATCTTCCTCGAGCGATTTTTCTAGCTTAAAAGCGCAGAAGCAAGATCCTATAATGCCTGGCAATGGTTCCAATGTAATGAATTTTTCCCATTTTGCAAGGCCTGCAGCTATTGTGAGAGCTAATCTTCAGAACATAGGCTTGAAGTCTGGTTTGAATTCAGCTTCAGCAAGATCAGATGTTATAGGAGTTAAGAATAAAGGCGCTGCTTCGACTAGCAGCAATCCTCCTGATTCAGCTCTTGTTAATCCAAGCGGTGAGTGTTCAAAGGAACCGGAAATGCGCTGTCATAAGGTGGTAGAGCCATCCAAGGCTGATTTGAAACCTTTGCAGCCGAAATTGCTTGAACCAAATGCTGTAGCTACTAAACAATCGAAGCCTGCTTGCAAAGAGAATGGAATTAAGAATGATCAACCTTCCATTCAAGTTCTTGGTGACAACGGCGCTAAAGGACACACAGCTGCTGAAAAAGGCATGGAGGTGGCAGTAGCCTCTTCTTCTGTTTGCTCTGGCAATGGTGCAGACAGAGGCTCGGATGATCCAAATCGAGATTTGAAACGGAAAAGTAGAGACACTGAGGACTCTGAATGCCATAGTGAAGATGTTGAGGATGAATCAGTGGGAGTTAAAAAGGGAGCGGCTGGACGAGGAGTTGCAGGTTCCAAAAGAAGCCGTGCTGCTGAAGTGCATAATCTATCTGAAAGGAGGCGAAGAGACAGGATAAATGAGAAGATGCGTGCATTGCAAGAACTCATACCTAATTGCAATAAGGTGGATAAAGCTTCTATGCTGGATGAGGCAATTGAGTATCTAAAAACACTTCAGCTCCAAGTTCAAATGATGTCAATGGGAGCTGGTTTATATATGCCTCAAATGATGTTACCTGCTGGAATGCAGCACATGCATGCACCACACATGGCCGCATTTTCACAGATGGGCATCGGCATGCATATGGGGTTAGGTATGGGTTATGGAATGGGAATGCCTGACATGAACGGCGGGCCGTCTAGATTTCCAATGATTCAAGTACCTCAGATGCAAGGAAATCATATGCCCGTTGCGCATATGCCTGGACCCTCTGCTATGCATGGAATGGCCAGATCGAATACTCAAGGGTTTGGACTTCCGGGACAGGGAATTTCCATGCCAATGCCGCATGGTCCGGCATTTCCTTTTTCAGGAGGGCCTCTTATGAACTCATCCGCTCCGGGTCCGTCTGTGTATGGATCCACAGGACATGTTGAAGCTGTGAATCCGGCTTCAGCGTGTGGCTCAAAAGATCGAAGGCCAGATGTGGACTCACAAATTAAGCAAAACACTGCTGGTCGTGACTCTACAAGCCAGATCCCTAACCAGTCAACTTTGGTGCATAATGGTGGCCATGCTTCAGTGGCTGATGACAGTGAAGCTGCTAATCCGGGCAATATAAATTTGTGA
Protein sequence

>MsaT004093.1

MPLYELYRLAREKLDNETNGASVADQPSSPENDFFELVWESGQISSQGQSSRGRKSPSCRSLPSHCLPSHSPRGRDKDVGGGFSNNNPKIGKFGDLDSGLNEIPMSVPSREVDEMMPWLDYSMDGSLQHEYGSDFFHELTENDLPASNSFTLLDKRSNGNQIFRDSHKNSAEPMNVSKGSSAEQVVETARHKASAGQLYPPSSNQCQTTSVTVRSRASDITENNNVSSGNQDVPYGDITRIPSSSSDFSSLKAQKQDPIMPGNGSNVMNFSHFARPAAIVRANLQNIGLKSGLNSASARSDVIGVKNKGAASTSSNPPDSALVNPSGECSKEPEMRCHKVVEPSKADLKPLQPKLLEPNAVATKQSKPACKENGIKNDQPSIQVLGDNGAKGHTAAEKGMEVAVASSSVCSGNGADRGSDDPNRDLKRKSRDTEDSECHSEDVEDESVGVKKGAAGRGVAGSKRSRAAEVHNLSERRRRDRINEKMRALQELIPNCNKVDKASMLDEAIEYLKTLQLQVQMMSMGAGLYMPQMMLPAGMQHMHAPHMAAFSQMGIGMHMGLGMGYGMGMPDMNGGPSRFPMIQVPQMQGNHMPVAHMPGPSAMHGMARSNTQGFGLPGQGISMPMPHGPAFPFSGGPLMNSSAPGPSVYGSTGHVEAVNPASACGSKDRRPDVDSQIKQNTAGRDSTSQIPNQSTLVHNGGHASVADDSEAANPGNINL*