AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004117


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004117 MtrunA17_Chr2g0296321 81.308 107 14 3 27 130 67 170 5.12e-55 172
MsaG004117 MtrunA17_Chr4g0067091 90.217 92 5 2 27 115 23 113 4.72e-54 169
MsaG004117 MtrunA17_Chr1g0195851 77.895 95 17 2 27 118 37 130 4.49e-50 158
MsaG004117 MtrunA17_Chr2g0290491 60.185 108 39 2 27 131 18 124 2.00e-41 135
MsaG004117 MtrunA17_Chr4g0076321 66.316 95 28 2 27 118 20 113 2.47e-41 134
MsaG004117 MtrunA17_Chr1g0191981 68.085 94 26 2 28 118 6 98 5.31e-41 132
MsaG004117 MtrunA17_Chr3g0102351 63.542 96 31 2 27 119 28 122 5.64e-40 132
MsaG004117 MtrunA17_Chr1g0185911 67.778 90 25 2 27 113 30 118 1.46e-39 130
MsaG004117 MtrunA17_Chr5g0446491 61.386 101 35 2 22 119 19 118 2.14e-39 130
MsaG004117 MtrunA17_Chr8g0382931 56.604 106 42 2 21 123 23 127 5.67e-39 130
MsaG004117 MtrunA17_Chr7g0263601 64.130 92 29 2 27 115 27 117 6.00e-39 129
MsaG004117 MtrunA17_Chr8g0384451 63.158 95 31 2 27 118 41 134 1.15e-38 129
MsaG004117 MtrunA17_Chr4g0076381 56.075 107 43 2 27 130 59 164 8.51e-37 125
MsaG004117 MtrunA17_Chr1g0165041 59.574 94 34 2 27 117 7 99 2.29e-33 115
MsaG004117 MtrunA17_Chr5g0446601 43.182 88 46 2 29 113 27 113 6.17e-18 75.9
MsaG004117 MtrunA17_Chr1g0159271 41.379 87 47 2 40 123 1 86 9.50e-16 67.8
MsaG004117 MtrunA17_Chr1g0158951 45.070 71 38 1 47 117 13 82 7.20e-15 65.9
MsaG004117 MtrunA17_Chr2g0307551 30.612 98 64 2 18 112 3 99 1.18e-13 63.9
MsaG004117 MtrunA17_Chr4g0062271 28.571 98 66 2 18 112 3 99 3.34e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004117 AT2G47810.1 72.277 101 24 2 21 118 46 145 3.90e-49 154
MsaG004117 AT1G09030.1 69.792 96 25 2 27 119 4 98 2.64e-43 139
MsaG004117 AT5G47640.1 66.337 101 30 2 22 119 23 122 1.23e-41 136
MsaG004117 AT4G14540.1 62.745 102 34 2 24 122 19 119 2.84e-41 135
MsaG004117 AT3G53340.3 60.952 105 37 2 21 122 24 127 3.97e-41 134
MsaG004117 AT3G53340.2 60.952 105 37 2 21 122 24 127 3.97e-41 134
MsaG004117 AT3G53340.5 63.265 98 32 2 21 115 24 120 9.20e-40 131
MsaG004117 AT3G53340.1 63.265 98 32 2 21 115 24 120 9.20e-40 131
MsaG004117 AT2G38880.3 67.391 92 26 2 27 115 22 112 1.04e-39 130
MsaG004117 AT2G38880.2 67.391 92 26 2 27 115 22 112 1.35e-39 130
MsaG004117 AT2G38880.1 67.391 92 26 2 27 115 22 112 1.35e-39 130
MsaG004117 AT2G38880.5 67.391 92 26 2 27 115 22 112 1.35e-39 130
MsaG004117 AT3G53340.4 62.887 97 32 2 21 114 24 119 4.45e-39 128
MsaG004117 AT2G37060.2 62.245 98 33 2 21 115 25 121 1.03e-38 129
MsaG004117 AT2G37060.3 62.245 98 33 2 21 115 25 121 1.03e-38 129
MsaG004117 AT2G37060.1 62.245 98 33 2 21 115 25 121 1.03e-38 129
MsaG004117 AT2G38880.4 65.934 91 27 2 27 114 22 111 2.13e-38 126
MsaG004117 AT2G38880.7 66.667 90 26 2 27 113 22 110 3.62e-38 126
MsaG004117 AT2G13570.1 64.211 95 30 2 27 118 37 130 4.53e-38 128
MsaG004117 AT2G38880.9 65.934 91 27 2 27 114 22 111 7.02e-38 126
MsaG004117 AT2G38880.10 65.934 91 27 2 27 114 22 111 7.02e-38 126
MsaG004117 AT2G38880.6 65.934 91 27 2 27 114 22 111 7.02e-38 126
MsaG004117 AT2G38880.11 65.934 91 27 2 27 114 22 111 7.20e-38 126
MsaG004117 AT2G38880.8 65.934 91 27 2 27 114 22 111 7.20e-38 126
MsaG004117 AT5G47670.2 61.053 95 33 2 27 118 30 123 5.37e-36 122
MsaG004117 AT5G47670.1 61.053 95 33 2 27 118 59 152 1.12e-35 122
MsaG004117 AT5G47670.3 61.053 95 33 2 27 118 59 152 1.12e-35 122
MsaG004117 AT1G21970.1 53.684 95 40 2 27 118 60 153 9.25e-31 110
MsaG004117 AT5G23090.4 28.037 107 71 3 18 119 3 108 6.36e-12 59.7
MsaG004117 AT5G23090.5 28.155 103 68 3 18 115 3 104 8.60e-11 56.6
MsaG004117 AT5G23090.2 28.155 103 68 3 18 115 3 104 8.60e-11 56.6
MsaG004117 AT5G23090.1 28.155 103 68 3 18 115 3 104 8.60e-11 56.6

Find 26 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCTTGCATTGTTTCCTT+TGG 0.194156 1:+65866489 None:intergenic
GCATAGATATAGAGAATTAG+AGG 0.332962 1:-65866316 MsaT004117.1:CDS
ACTCTTGCATTGTTTCCTTT+GGG 0.333372 1:+65866490 None:intergenic
CGATGATATATGTTGGGCTT+TGG 0.361762 1:-65866385 MsaT004117.1:CDS
GATGATATATGTTGGGCTTT+GGG 0.371039 1:-65866384 MsaT004117.1:CDS
TGAATATTATTAGTAGTAGT+TGG 0.374870 1:+65866282 None:intergenic
AACGTTGTTATCCATCGTTT+TGG 0.403126 1:+65866621 None:intergenic
ATCCTCCCAACATTTGCAAT+TGG 0.423560 1:+65866546 None:intergenic
TGTTGGGCTTTGGGGACACT+TGG 0.433132 1:-65866375 MsaT004117.1:CDS
TTGTTACCAATTGCAAATGT+TGG 0.438960 1:-65866552 MsaT004117.1:CDS
TGTTACCAATTGCAAATGTT+GGG 0.447901 1:-65866551 MsaT004117.1:CDS
TCCTTTAATAACGTTGAAAA+CGG 0.453836 1:-65866589 MsaT004117.1:CDS
ACTAATAATATTCAAAATAG+AGG 0.455896 1:-65866273 MsaT004117.1:CDS
GAAAGGAGAAAGACAGTGAA+TGG 0.488041 1:-65866411 MsaT004117.1:CDS
TGAAAACGGAGGCACAAGAT+AGG 0.505295 1:-65866575 MsaT004117.1:CDS
CAGAGAAATGCAGGAAGGAA+AGG 0.518431 1:-65866428 MsaT004117.1:CDS
ATGATATATGTTGGGCTTTG+GGG 0.541154 1:-65866383 MsaT004117.1:CDS
GTGACGCATCAGAGAAATGC+AGG 0.541912 1:-65866437 MsaT004117.1:CDS
TGGTGACGATGATATATGTT+GGG 0.557816 1:-65866391 MsaT004117.1:CDS
ACGATGGATAACAACGTTGG+AGG 0.595391 1:-65866616 MsaT004117.1:CDS
TTTAATAACGTTGAAAACGG+AGG 0.598517 1:-65866586 MsaT004117.1:CDS
TACCAATTGCAAATGTTGGG+AGG 0.600846 1:-65866548 MsaT004117.1:CDS
CGCATCAGAGAAATGCAGGA+AGG 0.623973 1:-65866433 MsaT004117.1:CDS
ATGGTGACGATGATATATGT+TGG 0.624511 1:-65866392 MsaT004117.1:CDS
AAAACGATGGATAACAACGT+TGG 0.647821 1:-65866619 MsaT004117.1:CDS
TAATAAAGGAACCAAAACGA+TGG 0.660654 1:-65866632 MsaT004117.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 65866266 65866736 65866266 ID=MsaG004117
Chr1 mRNA 65866266 65866736 65866266 ID=MsaT004117.1;Parent=MsaG004117
Chr1 exon 65866266 65866646 65866266 ID=MsaT004117.1.exon2;Parent=MsaT004117.1
Chr1 CDS 65866266 65866646 65866266 ID=cds.MsaT004117.1;Parent=MsaT004117.1
Chr1 exon 65866722 65866736 65866722 ID=MsaT004117.1.exon1;Parent=MsaT004117.1
Chr1 CDS 65866722 65866736 65866722 ID=cds.MsaT004117.1;Parent=MsaT004117.1
Gene Sequence

>MsaT004117.1

ATGAGACACGATGATGAACCAAAACGATGGATAACAACGTTGGAGGAAGTTCCTTTAATAACGTTGAAAACGGAGGCACAAGATAGGTTGTTACCAATTGCAAATGTTGGGAGGATAATGAAGCAGATATTACCACAGAATGCAAAAATCCCAAAGGAAACAATGCAAGAGTGTGTGTCAGAGTTTATAAGCTTTGTGACAAGTGACGCATCAGAGAAATGCAGGAAGGAAAGGAGAAAGACAGTGAATGGTGACGATGATATATGTTGGGCTTTGGGGACACTTGGTTTTGATGATTATGCTGAACAAATAAGAAAGTATTTGCATAGATATAGAGAATTAGAGGTAGAAAGAACACCAACTACTACTAATAATATTCAAAATAGAGGAAATTAA

Protein sequence

>MsaT004117.1

MRHDDEPKRWITTLEEVPLITLKTEAQDRLLPIANVGRIMKQILPQNAKIPKETMQECVSEFISFVTSDASEKCRKERRKTVNGDDDICWALGTLGFDDYAEQIRKYLHRYRELEVERTPTTTNNIQNRGN*