Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004125 | KEH43166.1 | 87.387 | 222 | 23 | 4 | 1 | 217 | 1 | 222 | 2.27e-115 | 341 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004125 | A0A072VNE8 | 87.387 | 222 | 23 | 4 | 1 | 217 | 1 | 222 | 1.09e-115 | 341 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG004125 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004125 | MtrunA17_Chr1g0193731 | 87.387 | 222 | 23 | 4 | 1 | 217 | 1 | 222 | 2.09e-119 | 341 |
| MsaG004125 | MtrunA17_Chr7g0214511 | 46.897 | 290 | 63 | 11 | 7 | 218 | 11 | 287 | 3.84e-61 | 192 |
| MsaG004125 | MtrunA17_Chr1g0180581 | 47.321 | 224 | 50 | 9 | 15 | 212 | 10 | 191 | 8.64e-46 | 151 |
| MsaG004125 | MtrunA17_Chr1g0169521 | 37.037 | 243 | 78 | 8 | 41 | 218 | 84 | 316 | 1.52e-29 | 112 |
| MsaG004125 | MtrunA17_Chr1g0174001 | 39.474 | 152 | 45 | 5 | 109 | 218 | 49 | 195 | 1.43e-20 | 85.5 |
| MsaG004125 | MtrunA17_Chr1g0172861 | 31.481 | 216 | 59 | 9 | 73 | 218 | 82 | 278 | 1.40e-13 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGAAGGTGAAACTGAATT+AGG | 0.203359 | 1:-65922297 | MsaT004125.1:CDS |
| TCAAAGTTCTTCACAACTTT+TGG | 0.220761 | 1:+65922273 | None:intergenic |
| AACTAAGATGAGGCACTAAT+TGG | 0.271486 | 1:+65922212 | None:intergenic |
| GAAGACAACAAAGAAGAAAA+AGG | 0.297955 | 1:-65922373 | MsaT004125.1:CDS |
| TCTATCTCGCGTGGGTTATA+AGG | 0.308553 | 1:-65922451 | MsaT004125.1:CDS |
| TGAATCACCGTGGTGCTAAA+AGG | 0.329449 | 1:-65922425 | MsaT004125.1:CDS |
| CCTGCACGGCTGCTGTTTCT+CGG | 0.336607 | 1:+65922542 | None:intergenic |
| CGCCATATGATCTTCTTCTC+AGG | 0.345803 | 1:+65922720 | None:intergenic |
| GTGCAGGAGAAGAAGATATA+TGG | 0.394772 | 1:-65922526 | MsaT004125.1:CDS |
| GAAAGAGAAAGAAGGGTATC+TGG | 0.402663 | 1:+65922782 | None:intergenic |
| ATGAAGGTGAAACTGAATTA+GGG | 0.412151 | 1:-65922296 | MsaT004125.1:CDS |
| ACTTCTAACAAAGCATTGAA+TGG | 0.414800 | 1:-65922615 | MsaT004125.1:CDS |
| GGCTGCTGTTTCTCGGAAGA+TGG | 0.453754 | 1:+65922549 | None:intergenic |
| CCGAGAAACAGCAGCCGTGC+AGG | 0.471067 | 1:-65922542 | MsaT004125.1:CDS |
| TCCTCAACAACTAATGATGC+TGG | 0.472142 | 1:-65922753 | MsaT004125.1:CDS |
| TCCAGCATCATTAGTTGTTG+AGG | 0.475624 | 1:+65922752 | None:intergenic |
| ACCCTGAGAAGAAGATCATA+TGG | 0.501896 | 1:-65922722 | MsaT004125.1:CDS |
| GAAGAGAAGAAGATTAATGA+AGG | 0.510676 | 1:-65922312 | MsaT004125.1:CDS |
| CTCATGTGATGTTTGCAACA+AGG | 0.514548 | 1:-65922643 | MsaT004125.1:CDS |
| TCTATTGCGTCTATCTCGCG+TGG | 0.518757 | 1:-65922460 | MsaT004125.1:CDS |
| ATGAGATGTCACGGTCAAAA+CGG | 0.520374 | 1:-65922588 | MsaT004125.1:CDS |
| TCATCTTCAAACTCATCACT+TGG | 0.524025 | 1:+65922237 | None:intergenic |
| GAAGATGGTGCTGCAACTGT+TGG | 0.540873 | 1:+65922564 | None:intergenic |
| GCCATATGATCTTCTTCTCA+GGG | 0.557187 | 1:+65922721 | None:intergenic |
| GACTCAACGAAAGAGAAAGA+AGG | 0.560928 | 1:+65922774 | None:intergenic |
| CGGAAAGAATGATCATGAGA+AGG | 0.580875 | 1:+65922843 | None:intergenic |
| CAAGGTGTGCTACTAATAAG+TGG | 0.587152 | 1:-65922348 | MsaT004125.1:CDS |
| ATATATCTTCTTCTCCTGCA+CGG | 0.589712 | 1:+65922528 | None:intergenic |
| ACTCAACGAAAGAGAAAGAA+GGG | 0.591599 | 1:+65922775 | None:intergenic |
| AATGGACATATGAGATGTCA+CGG | 0.619984 | 1:-65922597 | MsaT004125.1:CDS |
| CTATTGCGTCTATCTCGCGT+GGG | 0.626912 | 1:-65922459 | MsaT004125.1:CDS |
| GGTGTGCTACTAATAAGTGG+TGG | 0.729409 | 1:-65922345 | MsaT004125.1:CDS |
| TATAAGGATGTGAATCACCG+TGG | 0.764003 | 1:-65922435 | MsaT004125.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 65922227 | 65922883 | 65922227 | ID=MsaG004125 |
| Chr1 | mRNA | 65922227 | 65922883 | 65922227 | ID=MsaT004125.1;Parent=MsaG004125 |
| Chr1 | exon | 65922227 | 65922883 | 65922227 | ID=MsaT004125.1.exon1;Parent=MsaT004125.1 |
| Chr1 | CDS | 65922227 | 65922883 | 65922227 | ID=cds.MsaT004125.1;Parent=MsaT004125.1 |
| Gene Sequence |
| Protein sequence |
