AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004240 MtrunA17_Chr1g0194121 97.607 1003 24 0 1 1003 1 1003 0.0 2043
MsaG004240 MtrunA17_Chr7g0270541 75.347 1010 225 10 8 1003 2 1001 0.0 1497
MsaG004240 MtrunA17_Chr2g0302791 48.936 1034 474 19 7 1002 7 1024 0.0 907
MsaG004240 MtrunA17_Chr1g0162871 32.997 594 364 10 417 1002 435 1002 2.31e-86 299
MsaG004240 MtrunA17_Chr1g0162871 50.303 165 73 4 115 273 74 235 3.55e-38 154
MsaG004240 MtrunA17_Chr8g0360361 67.033 91 30 0 146 236 64 154 2.82e-33 126
MsaG004240 MtrunA17_Chr8g0334671 56.522 115 41 2 116 221 147 261 1.25e-32 129
MsaG004240 MtrunA17_Chr8g0386271 58.511 94 39 0 133 226 85 178 2.41e-31 127
MsaG004240 MtrunA17_Chr4g0046991 62.921 89 33 0 133 221 59 147 3.96e-31 119
MsaG004240 MtrunA17_Chr5g0420051 61.798 89 34 0 132 220 166 254 1.12e-30 126
MsaG004240 MtrunA17_Chr2g0282521 63.043 92 33 1 133 224 51 141 2.29e-30 117
MsaG004240 MtrunA17_Chr2g0308321 64.773 88 31 0 133 220 157 244 2.43e-30 127
MsaG004240 MtrunA17_Chr7g0231911 58.511 94 39 0 137 230 139 232 3.61e-30 122
MsaG004240 MtrunA17_Chr4g0061651 64.773 88 28 1 133 220 112 196 3.22e-29 122
MsaG004240 MtrunA17_Chr3g0122411 56.842 95 41 0 132 226 150 244 8.35e-29 120
MsaG004240 MtrunA17_Chr2g0286661 25.797 345 223 9 430 751 260 594 1.49e-28 123
MsaG004240 MtrunA17_Chr2g0286661 48.315 89 46 0 132 220 88 176 1.08e-20 98.2
MsaG004240 MtrunA17_Chr3g0132091 50.435 115 54 2 133 244 58 172 2.03e-28 117
MsaG004240 MtrunA17_Chr7g0224821 61.364 88 33 1 133 220 64 150 2.76e-28 117
MsaG004240 MtrunA17_Chr7g0258471 68.919 74 23 0 147 220 142 215 2.23e-27 116
MsaG004240 MtrunA17_Chr8g0377421 56.250 80 35 0 147 226 132 211 1.10e-23 102
MsaG004240 MtrunA17_Chr8g0377401 58.108 74 31 0 146 219 161 234 2.44e-21 94.7
MsaG004240 MtrunA17_Chr2g0317041 61.290 62 24 0 132 193 84 145 7.25e-20 87.4
MsaG004240 MtrunA17_Chr1g0175031 55.556 54 24 0 140 193 67 120 5.13e-13 67.4
MsaG004240 MtrunA17_Chr8g0377431 45.070 71 39 0 132 202 136 206 1.12e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004240 AT2G47070.1 56.604 1007 295 29 6 1003 8 881 0.0 994
MsaG004240 AT3G60030.1 56.392 1009 327 28 16 1003 11 927 0.0 986
MsaG004240 AT2G47070.2 58.383 841 253 21 171 1003 1 752 0.0 888
MsaG004240 AT1G20980.1 32.419 583 358 15 427 1002 481 1034 1.28e-78 278
MsaG004240 AT1G20980.1 41.393 244 94 7 26 260 38 241 1.24e-38 156
MsaG004240 AT1G76580.1 30.690 580 356 12 429 1002 480 1019 3.10e-76 271
MsaG004240 AT1G76580.1 35.273 275 103 7 30 297 40 246 1.64e-35 146
MsaG004240 AT1G76580.3 39.655 232 81 7 30 252 40 221 3.75e-36 147
MsaG004240 AT1G76580.3 36.239 218 131 4 429 644 480 691 3.85e-31 131
MsaG004240 AT1G76580.2 39.655 232 81 7 30 252 40 221 3.82e-36 147
MsaG004240 AT1G76580.2 35.909 220 133 4 429 646 480 693 1.00e-31 133
MsaG004240 AT2G33810.1 61.798 89 34 0 134 222 41 129 3.13e-32 122
MsaG004240 AT1G69170.1 65.909 88 30 0 133 220 110 197 6.55e-32 129
MsaG004240 AT1G02065.1 69.620 79 24 0 147 225 188 266 2.94e-31 126
MsaG004240 AT1G53160.2 60.606 99 33 2 134 226 35 133 6.52e-31 120
MsaG004240 AT1G53160.1 60.606 99 33 2 134 226 35 133 6.52e-31 120
MsaG004240 AT1G53160.3 60.606 99 33 2 134 226 35 133 8.23e-31 119
MsaG004240 AT3G15270.1 58.163 98 38 1 136 230 49 146 3.08e-30 118
MsaG004240 AT5G43270.3 56.701 97 42 0 147 243 169 265 6.94e-29 121
MsaG004240 AT5G43270.1 56.701 97 42 0 147 243 169 265 6.94e-29 121
MsaG004240 AT5G43270.2 56.701 97 42 0 147 243 169 265 6.94e-29 121
MsaG004240 AT5G50570.1 55.319 94 40 1 140 233 94 185 7.11e-29 119
MsaG004240 AT5G50570.2 55.319 94 40 1 140 233 94 185 7.11e-29 119
MsaG004240 AT5G50670.1 55.319 94 40 1 140 233 94 185 7.11e-29 119
MsaG004240 AT5G50670.2 55.319 94 40 1 140 233 94 185 7.11e-29 119
MsaG004240 AT1G27370.6 50.450 111 51 2 110 220 143 249 3.76e-28 117
MsaG004240 AT1G27370.1 50.450 111 51 2 110 220 143 249 5.19e-28 118
MsaG004240 AT1G27370.5 50.450 111 51 2 110 220 143 249 5.19e-28 118
MsaG004240 AT1G27370.3 50.450 111 51 2 110 220 143 249 5.19e-28 118
MsaG004240 AT1G27370.2 50.450 111 51 2 110 220 143 249 5.19e-28 118
MsaG004240 AT1G27370.4 50.450 111 51 2 110 220 143 249 5.19e-28 118
MsaG004240 AT1G27370.7 50.450 111 51 2 110 220 143 249 5.19e-28 118
MsaG004240 AT1G27360.2 50.450 111 50 2 110 220 143 248 1.60e-26 113
MsaG004240 AT1G27360.1 50.450 111 50 2 110 220 143 248 1.60e-26 113
MsaG004240 AT1G27360.3 50.450 111 50 2 110 220 143 248 1.60e-26 113
MsaG004240 AT1G27360.4 50.450 111 50 2 110 220 143 248 1.60e-26 113
MsaG004240 AT3G57920.1 58.242 91 38 0 130 220 42 132 5.48e-26 111
MsaG004240 AT5G18830.2 26.984 315 195 7 382 672 239 542 8.37e-26 115
MsaG004240 AT5G18830.2 54.545 88 39 1 133 220 125 211 2.51e-22 103
MsaG004240 AT5G18830.1 26.984 315 195 7 382 672 239 542 9.32e-26 115
MsaG004240 AT5G18830.1 54.545 88 39 1 133 220 125 211 2.53e-22 103
MsaG004240 AT5G18830.3 26.984 315 195 7 382 672 239 542 1.04e-25 114
MsaG004240 AT5G18830.3 54.545 88 39 1 133 220 125 211 2.66e-22 103
MsaG004240 AT2G42200.1 66.216 74 25 0 147 220 74 147 1.25e-25 110
MsaG004240 AT1G02065.2 68.750 48 15 0 147 194 188 235 6.79e-16 79.0

Find 246 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCTCTTGTTCTCGTAAA+AGG 0.174087 1:-67234732 None:intergenic
TGCAGTTCTACAAGAAATTT+TGG 0.179030 1:-67238446 None:intergenic
GCAACAATTATAGCAAAATT+TGG 0.200968 1:+67236426 MsaT004240.1:CDS
TTTCCAGTCCCTTCAATATC+TGG 0.226153 1:-67237248 None:intergenic
ACCAACAAGCGCCTTGTCTT+TGG 0.258264 1:-67237621 None:intergenic
AGAACGGTTCACATGGCTTA+TGG 0.294581 1:+67237388 MsaT004240.1:CDS
AGATCTTGAGAAAGGGAATA+AGG 0.299596 1:+67231531 MsaT004240.1:CDS
GAAATCCATAGCTTGACTTT+TGG 0.299906 1:-67237279 None:intergenic
GGGAATGGAATTTGAATGAT+TGG 0.316466 1:+67231359 MsaT004240.1:CDS
TATGGATTTCATTCATGAAA+TGG 0.320879 1:+67237292 MsaT004240.1:CDS
TTTATTCTCCAATGGTTCTC+AGG 0.328039 1:+67234801 MsaT004240.1:CDS
TTTGATGAAACTGTTAATAA+AGG 0.328176 1:+67237467 MsaT004240.1:CDS
ACAGAAGCCAATTGAAATAT+AGG 0.336809 1:+67237327 MsaT004240.1:CDS
ACCTTGTGGCAGGGCGCATC+AGG 0.339254 1:-67236511 None:intergenic
GCACTTAGCTTAAATCTTGC+AGG 0.340035 1:+67231616 MsaT004240.1:CDS
CCAACAAGCGCCTTGTCTTT+GGG 0.348507 1:-67237620 None:intergenic
TCATTTGTTGGACTTCCATT+AGG 0.355984 1:-67233270 None:intergenic
AGTTTGTGAGATGCATTCTA+AGG 0.361547 1:+67231774 MsaT004240.1:CDS
CCCACTCCGGTCTGGGATCC+CGG 0.362139 1:+67231462 MsaT004240.1:CDS
CTCGGCCTGACGCAGATAAA+TGG 0.364403 1:-67236037 None:intergenic
ATCTATTAACTCATCTTCTA+AGG 0.366559 1:+67234662 MsaT004240.1:CDS
AGCCATGTGAACCGTTCTAA+TGG 0.369029 1:-67237383 None:intergenic
CAGACCTGCAATGCTCTCTA+TGG 0.376267 1:+67238489 MsaT004240.1:CDS
ATTGCTCTCTGTCGTATTGA+TGG 0.383082 1:-67238332 None:intergenic
TAACCATCCTATATTTCAAT+TGG 0.385181 1:-67237334 None:intergenic
CTAAGTGAATGTAAGTATAA+TGG 0.386411 1:-67238255 None:intergenic
TTTGATGCCGGTACAGCAAT+TGG 0.388018 1:-67236458 None:intergenic
GAGAAATAGCAAACAGTTAG+TGG 0.388325 1:+67237550 MsaT004240.1:CDS
TTCCAGTTTGATTAAGCTGC+TGG 0.388701 1:+67236195 MsaT004240.1:CDS
AGGTTAATGTGTGCTTTAGA+AGG 0.390854 1:+67236688 MsaT004240.1:intron
CGGAACATGTATAGAACTTC+GGG 0.394622 1:-67238562 None:intergenic
ACCTTTCAGATGGGAATCAT+TGG 0.397557 1:+67238585 MsaT004240.1:CDS
AATCAAGAAGCTGTTCCTAA+TGG 0.397811 1:+67233255 MsaT004240.1:CDS
GCTTCTCCGCTAGAACTAGA+AGG 0.400011 1:-67235189 None:intergenic
CTAGTCTGATCATCATTTGT+TGG 0.400423 1:-67233282 None:intergenic
AGTACAAGAGGAAATTCATT+TGG 0.402660 1:-67235844 None:intergenic
CAGGTACAGAACATGAAAAT+TGG 0.403924 1:-67236786 None:intergenic
ATCCAGCAGCTTAATCAAAC+TGG 0.406521 1:-67236197 None:intergenic
GCCACCATAGAGAGCATTGC+AGG 0.412655 1:-67238493 None:intergenic
AGATGCATTCTAAGGCTAGT+AGG 0.412881 1:+67231782 MsaT004240.1:CDS
GAATCAAATGCAACAAGAAA+TGG 0.413695 1:+67234864 MsaT004240.1:CDS
ATGTCTCAGATGATACGTTT+TGG 0.415679 1:+67236218 MsaT004240.1:CDS
CTCTTGCTTCCGAGTAAGCC+GGG 0.419245 1:-67234960 None:intergenic
TGCTTTAGAAGGAAAATATC+TGG 0.420117 1:+67236699 MsaT004240.1:CDS
TCATTCACTAGCTTAGAGAT+TGG 0.421089 1:+67238376 MsaT004240.1:CDS
ATGGATTTCATTCATGAAAT+GGG 0.424476 1:+67237293 MsaT004240.1:CDS
TCATCTGATTTGAGTGGGAT+GGG 0.427804 1:+67231325 MsaT004240.1:CDS
TTCATCTGATTTGAGTGGGA+TGG 0.429528 1:+67231324 MsaT004240.1:CDS
CAAGAGAATTTGTTGAGAGA+AGG 0.430841 1:+67234745 MsaT004240.1:CDS
TTTGACCATTTATCTGCGTC+AGG 0.436128 1:+67236032 MsaT004240.1:CDS
CCTGATGCTGTTGGTCCTGC+CGG 0.436534 1:+67237671 MsaT004240.1:CDS
AAGAGAATTTGTTGAGAGAA+GGG 0.437952 1:+67234746 MsaT004240.1:CDS
AGATGGTTCCGAGGATGTAC+TGG 0.438143 1:+67237724 MsaT004240.1:CDS
CGCTAGAACTAGAAGGGGAC+TGG 0.439772 1:-67235182 None:intergenic
CGGTCTGGGATCCCGGTGGT+TGG 0.446088 1:+67231469 MsaT004240.1:CDS
CCCAAAGACAAGGCGCTTGT+TGG 0.448364 1:+67237620 MsaT004240.1:CDS
AGCTGTCGAAGACGCTTGGC+TGG 0.450145 1:+67233207 MsaT004240.1:CDS
TCAATTGGCAGATTCTAGAT+TGG 0.452129 1:+67235956 MsaT004240.1:intron
AGATGGGAATCATTGGAGTT+CGG 0.452671 1:+67238592 MsaT004240.1:CDS
TTCGAATCGAGCTGTCTGTC+AGG 0.452984 1:+67231696 MsaT004240.1:CDS
TCTGAATTTCCACTTGTTTG+AGG 0.453259 1:-67235147 None:intergenic
GTGGGATGGGGAGGAGGTCA+AGG 0.453462 1:+67231338 MsaT004240.1:CDS
TATAGCTAGCCGTGTGAATC+CGG 0.453788 1:+67231402 MsaT004240.1:CDS
CGCAAACTCTCCGCCTGCAC+CGG 0.454733 1:-67231421 None:intergenic
CATTTATCTGCGTCAGGCCG+AGG 0.455554 1:+67236038 MsaT004240.1:CDS
GTCAGATCAGCCTACAGATC+AGG 0.456789 1:+67234639 MsaT004240.1:CDS
TCTGGGATCCCGGTGGTTGG+AGG 0.462646 1:+67231472 MsaT004240.1:CDS
TTTCCGTCCCTTTCCACAGC+CGG 0.462810 1:-67231649 None:intergenic
CATCTGATTTGAGTGGGATG+GGG 0.463932 1:+67231326 MsaT004240.1:CDS
TTATTCTCCAATGGTTCTCA+GGG 0.464671 1:+67234802 MsaT004240.1:CDS
TTAAATTAATTATAGCTGAC+AGG 0.464842 1:+67234617 MsaT004240.1:intron
TTGCTCTCTGTCGTATTGAT+GGG 0.466728 1:-67238331 None:intergenic
AGGATGGTTAACTTGAACTC+TGG 0.466960 1:+67237347 MsaT004240.1:CDS
ATAAGGAAGGGGATAGGAAA+AGG 0.467611 1:+67231548 MsaT004240.1:CDS
AAGGGAATAAGGAAGGGGAT+AGG 0.469713 1:+67231542 MsaT004240.1:CDS
TCTCTTGCTTCCGAGTAAGC+CGG 0.470159 1:-67234961 None:intergenic
CCGGCAGGACCAACAGCATC+AGG 0.471054 1:-67237671 None:intergenic
ATTTGAGTGGGATGGGGAGG+AGG 0.473356 1:+67231332 MsaT004240.1:CDS
CTTGAGAAAGGGAATAAGGA+AGG 0.476247 1:+67231535 MsaT004240.1:CDS
TTGATTACAGGTGGGAATTG+AGG 0.477112 1:+67238171 MsaT004240.1:intron
TCCTCTTGTACTTAGAGCAC+AGG 0.478174 1:+67235855 MsaT004240.1:CDS
TGGGGAGGAGGTCAAGGGAA+TGG 0.478218 1:+67231344 MsaT004240.1:CDS
AACAGAGATTACCTTGTGGC+AGG 0.479919 1:-67236521 None:intergenic
TGCGTCAGGCCGAGGCTGTG+TGG 0.480436 1:+67236046 MsaT004240.1:CDS
AACTGCAGTAGGAAGGTCTA+TGG 0.480847 1:+67238462 MsaT004240.1:CDS
TCCTCTTCTGCCACTATGAA+AGG 0.481801 1:-67237170 None:intergenic
CATATAGCAGCGGGAAAAGA+TGG 0.484670 1:+67237707 MsaT004240.1:CDS
TATCAGAGTGCAACATCAAA+TGG 0.485125 1:+67236258 MsaT004240.1:CDS
AAAGACAAGGCGCTTGTTGG+TGG 0.485410 1:+67237623 MsaT004240.1:CDS
AGGACATGTTTCACCGGCTG+TGG 0.490256 1:+67231636 MsaT004240.1:CDS
CTAGCTCTGTTGACTACCCT+TGG 0.491004 1:+67235094 MsaT004240.1:CDS
TGTTTCACCGGCTGTGGAAA+GGG 0.493285 1:+67231642 MsaT004240.1:CDS
TAAGGCTAGTAGGGCTCTTG+TGG 0.497571 1:+67231792 MsaT004240.1:CDS
TGTTAGTCCAATTGCTGTAC+CGG 0.498218 1:+67236451 MsaT004240.1:CDS
GGAATTTGAATGATTGGAGA+TGG 0.498797 1:+67231365 MsaT004240.1:CDS
CACAAAGTTTACAGTTCCCT+CGG 0.498820 1:-67238420 None:intergenic
GAAAGTGCTTGATACAATGT+AGG 0.501114 1:-67237497 None:intergenic
GATTTGCTAGATTGAGGACC+AGG 0.501170 1:+67237127 MsaT004240.1:intron
GTATCAGCTTTATTCTCCAA+TGG 0.501723 1:+67234793 MsaT004240.1:CDS
TGGAGACCCCTGAGAACCAT+TGG 0.509467 1:-67234809 None:intergenic
TAACAGTTTCATCAAAGAGT+AGG 0.509576 1:-67237460 None:intergenic
TCCTTTCATAGTGGCAGAAG+AGG 0.510150 1:+67237169 MsaT004240.1:CDS
GAGAAGCTGTCGAAGACGCT+TGG 0.511016 1:+67233203 MsaT004240.1:CDS
CTCAATATCAGTAGGACTGT+GGG 0.513581 1:-67235983 None:intergenic
GAGGAAGGAGATCTTGAGAA+AGG 0.514583 1:+67231523 MsaT004240.1:CDS
GTGGTTCATCTGATTTGAGT+GGG 0.514851 1:+67231320 MsaT004240.1:CDS
GGAGCATGAAGAAGACGTAT+TGG 0.515867 1:-67231500 None:intergenic
AGTTCATCAGATGTATTGTC+AGG 0.517811 1:-67237593 None:intergenic
TAGCCGTGTGAATCCGGTGC+AGG 0.517976 1:+67231408 MsaT004240.1:CDS
GCGTCAGGCCGAGGCTGTGT+GGG 0.518427 1:+67236047 MsaT004240.1:CDS
TAAAGCCAAAAGTCAAGCTA+TGG 0.519399 1:+67237274 MsaT004240.1:CDS
TATATGAAGTGGTGTCAAAC+CGG 0.519627 1:-67237690 None:intergenic
TCACCACTGCACACCAATCA+TGG 0.519744 1:-67237424 None:intergenic
TAGTTCTAGCGGAGAAGCCC+AGG 0.520957 1:+67235194 MsaT004240.1:CDS
ATGTTTCACCGGCTGTGGAA+AGG 0.521156 1:+67231641 MsaT004240.1:CDS
TTTCTCAAGATCTCCTTCCT+CGG 0.522636 1:-67231521 None:intergenic
GAAACTGATCCAGATATTGA+AGG 0.522834 1:+67237239 MsaT004240.1:CDS
TTGGAGTTCGGAACAAGCTA+AGG 0.522976 1:+67238604 MsaT004240.1:CDS
TCGGAACATGTATAGAACTT+CGG 0.525195 1:-67238563 None:intergenic
GTCTTCTTCACAGAGCTGTA+AGG 0.525330 1:+67237528 MsaT004240.1:CDS
CAACCACCGGGATCCCAGAC+CGG 0.527167 1:-67231468 None:intergenic
AAGAATGCCCGTGACAGTAC+AGG 0.527708 1:+67238199 MsaT004240.1:CDS
AGAGAATTTGTTGAGAGAAG+GGG 0.527845 1:+67234747 MsaT004240.1:CDS
GTTTCACTTGATTCTAGTAG+AGG 0.528154 1:-67237221 None:intergenic
ATTTCTTGTAGAACTGCAGT+AGG 0.528560 1:+67238451 MsaT004240.1:CDS
GAAAAGGAGAGTTATTGTGC+TGG 0.529228 1:+67231564 MsaT004240.1:CDS
GTGTGGAGCCTGTACTGTCA+CGG 0.530352 1:-67238207 None:intergenic
ACATGTTCCGACCTTTCAGA+TGG 0.530501 1:+67238575 MsaT004240.1:CDS
CCGGGATCCCAGACCGGAGT+GGG 0.530624 1:-67231462 None:intergenic
TGATGTTGTGTTATAACAGT+TGG 0.532386 1:-67234829 None:intergenic
GCTTGGCTGGCCATAACAAA+CGG 0.533402 1:+67233220 MsaT004240.1:CDS
ACCTGTGCTCTAAGTACAAG+AGG 0.533970 1:-67235856 None:intergenic
TATCAAGCACTTTCAGAGAC+GGG 0.534522 1:+67237506 MsaT004240.1:CDS
ATTTCATTCATGAAATGGGC+TGG 0.535051 1:+67237297 MsaT004240.1:CDS
GAATTTGAATGATTGGAGAT+GGG 0.536595 1:+67231366 MsaT004240.1:CDS
TTTCCCGCTGCTATATGAAG+TGG 0.537198 1:-67237701 None:intergenic
GATCCAGATATTGAAGGGAC+TGG 0.538378 1:+67237245 MsaT004240.1:CDS
GTTATTGTGCTGGAAGATGA+TGG 0.539294 1:+67231574 MsaT004240.1:CDS
TGTTCTGTACCTGTGACAAA+TGG 0.539622 1:+67236796 MsaT004240.1:CDS
TAGATCTCTTTCCATTAGAA+CGG 0.540024 1:+67237372 MsaT004240.1:CDS
CTGATTTGAGTGGGATGGGG+AGG 0.540249 1:+67231329 MsaT004240.1:CDS
GGGGTTCTTCAAGAAATTCA+GGG 0.541114 1:+67234767 MsaT004240.1:CDS
GAATTTCCACTTGTTTGAGG+TGG 0.542056 1:-67235144 None:intergenic
TTGTTTAGACCTGATGCTGT+TGG 0.542526 1:+67237662 MsaT004240.1:CDS
CGTAATCGCGCATAATCCTC+AGG 0.544188 1:-67238229 None:intergenic
ATATATGTTGACTCAGATGA+TGG 0.545000 1:+67235027 MsaT004240.1:CDS
AGGAAGGAGATCTTGAGAAA+GGG 0.547901 1:+67231524 MsaT004240.1:CDS
GTAGCAAGATTTGTAGAGAC+GGG 0.551066 1:-67235072 None:intergenic
TTGGACGAACCTCCAACCAC+CGG 0.551797 1:-67231481 None:intergenic
CTTCTCCGCTAGAACTAGAA+GGG 0.552259 1:-67235188 None:intergenic
TGAGAAAGGGAATAAGGAAG+GGG 0.552930 1:+67231537 MsaT004240.1:CDS
AATCCTCTTCCATTTGTCAC+AGG 0.553594 1:-67236805 None:intergenic
ACACCACTTCATATAGCAGC+GGG 0.553941 1:+67237698 MsaT004240.1:CDS
GATGCATTCTAAGGCTAGTA+GGG 0.556125 1:+67231783 MsaT004240.1:CDS
AATTGAGCTCTCTTTGATGC+CGG 0.556172 1:-67236470 None:intergenic
GTTAATAGATCCTGATCTGT+AGG 0.556745 1:-67234649 None:intergenic
TGGGATGGGGAGGAGGTCAA+GGG 0.558672 1:+67231339 MsaT004240.1:CDS
TCTTCTTCATGCTCCGAGGA+AGG 0.558761 1:+67231508 MsaT004240.1:CDS
AGTAGCAAGATTTGTAGAGA+CGG 0.559291 1:-67235073 None:intergenic
TTGAATGATTGGAGATGGGA+TGG 0.559506 1:+67231370 MsaT004240.1:CDS
CTGAAGTTCATCAAGCTCCT+CGG 0.559955 1:-67236758 None:intergenic
AAGGCTAGTAGGGCTCTTGT+GGG 0.562216 1:+67231793 MsaT004240.1:CDS
ACCTGATGCGCCCTGCCACA+AGG 0.562538 1:+67236510 MsaT004240.1:CDS
ATACTTCAAGAGTTCGATGA+AGG 0.564195 1:+67233177 MsaT004240.1:CDS
CTTGTAGAACTGCAGTAGGA+AGG 0.568836 1:+67238455 MsaT004240.1:CDS
CAGCTTCTTTCCTTTCATAG+TGG 0.569410 1:+67237160 MsaT004240.1:CDS
TTCCATTAGAACGGTTCACA+TGG 0.572870 1:+67237381 MsaT004240.1:CDS
TGAAGTGGTGTCAAACCGGC+AGG 0.572947 1:-67237686 None:intergenic
CAGCATTTCAAACAGTCCCC+CGG 0.574192 1:+67234942 MsaT004240.1:CDS
GAGAATTTGTTGAGAGAAGG+GGG 0.576069 1:+67234748 MsaT004240.1:CDS
TCAGGCCGAGGCTGTGTGGG+AGG 0.577431 1:+67236050 MsaT004240.1:CDS
CCGTGTGAATCCGGTGCAGG+CGG 0.578551 1:+67231411 MsaT004240.1:CDS
TGTGGAGCCTGTACTGTCAC+GGG 0.578816 1:-67238206 None:intergenic
AGAAGCTGCTACTCAAACCC+TGG 0.580967 1:-67237145 None:intergenic
ATGTAGCTCTCAATATCAGT+AGG 0.583286 1:-67235991 None:intergenic
GTATCAAGCACTTTCAGAGA+CGG 0.583452 1:+67237505 MsaT004240.1:CDS
TGCAGATCTGAGTAGAGGTA+AGG 0.583887 1:+67231732 MsaT004240.1:CDS
CTATAATTGTTGCTTCGGAA+AGG 0.584009 1:-67236416 None:intergenic
TCTTGCTTCCGAGTAAGCCG+GGG 0.584021 1:-67234959 None:intergenic
TTGAGAAAGGGAATAAGGAA+GGG 0.585696 1:+67231536 MsaT004240.1:CDS
TCAAGTCCACCTCAAACAAG+TGG 0.586688 1:+67235138 MsaT004240.1:CDS
GGTGGTTCATCTGATTTGAG+TGG 0.587139 1:+67231319 MsaT004240.1:CDS
ACAGAGATTACCTTGTGGCA+GGG 0.588689 1:-67236520 None:intergenic
CAGAGCATCCAGTACATCCT+CGG 0.589070 1:-67237732 None:intergenic
ACAAACACAGACCGCTGCTA+TGG 0.592403 1:-67238515 None:intergenic
CTCTATGGTGGCCATAGCAG+CGG 0.592561 1:+67238504 MsaT004240.1:CDS
GTACCTGTGACAAATGGAAG+AGG 0.592712 1:+67236802 MsaT004240.1:CDS
ATGATTCCCATCTGAAAGGT+CGG 0.593018 1:-67238582 None:intergenic
AGATATGAGTTGATGATCAG+AGG 0.595345 1:-67234908 None:intergenic
AAGCCAATTGAAATATAGGA+TGG 0.596851 1:+67237331 MsaT004240.1:CDS
TCCAATGATTCCCATCTGAA+AGG 0.600424 1:-67238586 None:intergenic
TCTTGCAGGACATGTTTCAC+CGG 0.602620 1:+67231630 MsaT004240.1:CDS
GCTAGAACTAGAAGGGGACT+GGG 0.602647 1:-67235181 None:intergenic
TGAGAATCTTGTTGTGTCCA+AGG 0.603103 1:-67235111 None:intergenic
ATTGAGAGCTACATACGCCC+TGG 0.604603 1:+67236000 MsaT004240.1:CDS
AAATGGAAGAGGATTTATTG+AGG 0.605184 1:+67236813 MsaT004240.1:CDS
CATGTTCCGACCTTTCAGAT+GGG 0.605353 1:+67238576 MsaT004240.1:CDS
TGGACGAACCTCCAACCACC+GGG 0.606579 1:-67231480 None:intergenic
AACACAAGGAGCAGCTCATG+TGG 0.607987 1:+67238297 MsaT004240.1:CDS
TTCTTCAAGAAATTCAGGGA+TGG 0.609933 1:+67234771 MsaT004240.1:CDS
AAACTGATCCAGATATTGAA+GGG 0.611495 1:+67237240 MsaT004240.1:CDS
CAGTCCCCTTCTAGTTCTAG+CGG 0.612309 1:+67235183 MsaT004240.1:CDS
TACTTCCTCCCACACAGCCT+CGG 0.612916 1:-67236055 None:intergenic
TGAAGATGCTCATGAGTCAA+CGG 0.614117 1:+67236726 MsaT004240.1:CDS
GAGGATTATGCGCGATTACG+TGG 0.614195 1:+67238232 MsaT004240.1:CDS
GTCTGTCAGGTTGAAGACTG+TGG 0.617577 1:+67231709 MsaT004240.1:CDS
CTGGCCATAACAAACGGAGA+AGG 0.618872 1:+67233226 MsaT004240.1:CDS
TCACCGGCTGTGGAAAGGGA+CGG 0.619862 1:+67231646 MsaT004240.1:CDS
GACAATACATCTGATGAACT+AGG 0.621300 1:+67237596 MsaT004240.1:CDS
CAGGCGGAGAGTTTGCGAGT+GGG 0.622495 1:+67231427 MsaT004240.1:CDS
TACAGGTGGGAATTGAGGCA+TGG 0.622502 1:+67238176 MsaT004240.1:intron
CCGCCTGCACCGGATTCACA+CGG 0.624536 1:-67231411 None:intergenic
GCAGGCGGAGAGTTTGCGAG+TGG 0.624882 1:+67231426 MsaT004240.1:CDS
TCTCAATATCAGTAGGACTG+TGG 0.625316 1:-67235984 None:intergenic
GCAAGTCAGAATGATGAACA+AGG 0.626908 1:+67234691 MsaT004240.1:CDS
ACCTGCAATGCTCTCTATGG+TGG 0.630335 1:+67238492 MsaT004240.1:CDS
TTGTGGGAAATGCAATGCAG+AGG 0.630557 1:+67231809 MsaT004240.1:CDS
GCTGAAGTAAGACGTAGCCG+AGG 0.633383 1:+67238403 MsaT004240.1:CDS
ATGGTCAAAACAATACAACC+AGG 0.638951 1:-67236018 None:intergenic
GACACCACTTCATATAGCAG+CGG 0.639223 1:+67237697 MsaT004240.1:CDS
TGTTGACTCAGATGATGGAA+CGG 0.641750 1:+67235032 MsaT004240.1:CDS
TCTGACTAATGATCCTTGCA+TGG 0.643398 1:+67237751 MsaT004240.1:CDS
GATGGTTTGAATGATGAAGC+TGG 0.645206 1:+67231592 MsaT004240.1:CDS
TATTCTCCAATGGTTCTCAG+GGG 0.647451 1:+67234803 MsaT004240.1:CDS
TTCTCCGCTAGAACTAGAAG+GGG 0.647976 1:-67235187 None:intergenic
TCGCGCATAATCCTCAGGTG+TGG 0.653531 1:-67238224 None:intergenic
AGCAGCGGTCTGTGTTTGTG+TGG 0.653598 1:+67238519 MsaT004240.1:CDS
CTAGTAGAGGTTCAAGCACG+CGG 0.656034 1:-67237208 None:intergenic
ATTTGCTAGATTGAGGACCA+GGG 0.658771 1:+67237128 MsaT004240.1:intron
AAAGTGCTTGATACAATGTA+GGG 0.660121 1:-67237496 None:intergenic
CTGAAGTAAGACGTAGCCGA+GGG 0.663241 1:+67238404 MsaT004240.1:CDS
TGAACTAGGACCCAAAGACA+AGG 0.663671 1:+67237610 MsaT004240.1:CDS
AGCGGGAAAAGATGGTTCCG+AGG 0.663787 1:+67237715 MsaT004240.1:CDS
TACGTCTTCTTCATGCTCCG+AGG 0.664152 1:+67231504 MsaT004240.1:CDS
ACCGGGATCCCAGACCGGAG+TGG 0.666079 1:-67231463 None:intergenic
TGTGGTGCAGATCTGAGTAG+AGG 0.668323 1:+67231727 MsaT004240.1:CDS
CAGTACAGGCTCCACACCTG+AGG 0.669316 1:+67238213 MsaT004240.1:CDS
ACAAGGAGCAGCTCATGTGG+TGG 0.675515 1:+67238300 MsaT004240.1:CDS
GAGAATCTTGTTGTGTCCAA+GGG 0.677067 1:-67235110 None:intergenic
CAAGTCAGAATGATGAACAA+GGG 0.681362 1:+67234692 MsaT004240.1:CDS
ACTCCGGTCTGGGATCCCGG+TGG 0.685385 1:+67231465 MsaT004240.1:CDS
TGGTCAAAACAATACAACCA+GGG 0.689970 1:-67236017 None:intergenic
GGACCATGATTGGTGTGCAG+TGG 0.690358 1:+67237421 MsaT004240.1:CDS
CTTGCTTCCGAGTAAGCCGG+GGG 0.690508 1:-67234958 None:intergenic
TCGGAAGCAAGAGATAGCAG+TGG 0.695304 1:+67234970 MsaT004240.1:CDS
GTCAACGGATCAATACTCCG+AGG 0.781576 1:+67236741 MsaT004240.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 67231274 67238624 67231274 ID=MsaG004240
Chr1 mRNA 67231274 67238624 67231274 ID=MsaT004240.1;Parent=MsaG004240
Chr1 exon 67231274 67231851 67231274 ID=MsaT004240.1.exon1;Parent=MsaT004240.1
Chr1 CDS 67231274 67231851 67231274 ID=cds.MsaT004240.1;Parent=MsaT004240.1
Chr1 exon 67233170 67233351 67233170 ID=MsaT004240.1.exon2;Parent=MsaT004240.1
Chr1 CDS 67233170 67233351 67233170 ID=cds.MsaT004240.1;Parent=MsaT004240.1
Chr1 exon 67234632 67235215 67234632 ID=MsaT004240.1.exon3;Parent=MsaT004240.1
Chr1 CDS 67234632 67235215 67234632 ID=cds.MsaT004240.1;Parent=MsaT004240.1
Chr1 exon 67235802 67235876 67235802 ID=MsaT004240.1.exon4;Parent=MsaT004240.1
Chr1 CDS 67235802 67235876 67235802 ID=cds.MsaT004240.1;Parent=MsaT004240.1
Chr1 exon 67235967 67236074 67235967 ID=MsaT004240.1.exon5;Parent=MsaT004240.1
Chr1 CDS 67235967 67236074 67235967 ID=cds.MsaT004240.1;Parent=MsaT004240.1
Chr1 exon 67236178 67236295 67236178 ID=MsaT004240.1.exon6;Parent=MsaT004240.1
Chr1 CDS 67236178 67236295 67236178 ID=cds.MsaT004240.1;Parent=MsaT004240.1
Chr1 exon 67236390 67236531 67236390 ID=MsaT004240.1.exon7;Parent=MsaT004240.1
Chr1 CDS 67236390 67236531 67236390 ID=cds.MsaT004240.1;Parent=MsaT004240.1
Chr1 exon 67236690 67236834 67236690 ID=MsaT004240.1.exon8;Parent=MsaT004240.1
Chr1 CDS 67236690 67236834 67236690 ID=cds.MsaT004240.1;Parent=MsaT004240.1
Chr1 exon 67237137 67237772 67237137 ID=MsaT004240.1.exon9;Parent=MsaT004240.1
Chr1 CDS 67237137 67237772 67237137 ID=cds.MsaT004240.1;Parent=MsaT004240.1
Chr1 exon 67238181 67238624 67238181 ID=MsaT004240.1.exon10;Parent=MsaT004240.1
Chr1 CDS 67238181 67238624 67238181 ID=cds.MsaT004240.1;Parent=MsaT004240.1
Gene Sequence

>MsaT004240.1

ATGGGGGAAAGGTTGGGTGCTGAAAATTATCATTTTTATGGTGTTGGTGGTTCATCTGATTTGAGTGGGATGGGGAGGAGGTCAAGGGAATGGAATTTGAATGATTGGAGATGGGATGGTGATTTGTTTATAGCTAGCCGTGTGAATCCGGTGCAGGCGGAGAGTTTGCGAGTGGGACAACAGTTTTTCCCACTCCGGTCTGGGATCCCGGTGGTTGGAGGTTCGTCCAATACGTCTTCTTCATGCTCCGAGGAAGGAGATCTTGAGAAAGGGAATAAGGAAGGGGATAGGAAAAGGAGAGTTATTGTGCTGGAAGATGATGGTTTGAATGATGAAGCTGGTGCACTTAGCTTAAATCTTGCAGGACATGTTTCACCGGCTGTGGAAAGGGACGGAAAAAAGAGCAGAGTAGCTGGAGGAACTTCGAATCGAGCTGTCTGTCAGGTTGAAGACTGTGGTGCAGATCTGAGTAGAGGTAAGGATTATCACAGACGTCATAAAGTTTGTGAGATGCATTCTAAGGCTAGTAGGGCTCTTGTGGGAAATGCAATGCAGAGGTTTTGTCAACAGTGTAGTAGGTTTCACATACTTCAAGAGTTCGATGAAGGAAAGAGAAGCTGTCGAAGACGCTTGGCTGGCCATAACAAACGGAGAAGGAAAACAAATCAAGAAGCTGTTCCTAATGGAAGTCCAACAAATGATGATCAGACTAGTAGTTATCTGTTGATTAGTTTATTGAAGATACTTTCAAATATGCATTCTGACAGGTCAGATCAGCCTACAGATCAGGATCTATTAACTCATCTTCTAAGGAGTCTTGCAAGTCAGAATGATGAACAAGGGAGCAAGAACTTGTCAAACCTTTTACGAGAACAAGAGAATTTGTTGAGAGAAGGGGGTTCTTCAAGAAATTCAGGGATGGTATCAGCTTTATTCTCCAATGGTTCTCAGGGGTCTCCAACTGTTATAACACAACATCAAACTGTATCTATGAATCAAATGCAACAAGAAATGGTGCATACACATGATGTTAGAACCTCTGATCATCAACTCATATCTTCAATAAAACCCAGCATTTCAAACAGTCCCCCGGCTTACTCGGAAGCAAGAGATAGCAGTGGACAAACAAAGATGAATAATTTTGATTTGAATGACATATATGTTGACTCAGATGATGGAACGGAAGATCTAGAAAGACTGCCCGTCTCTACAAATCTTGCTACTAGCTCTGTTGACTACCCTTGGACACAACAAGATTCTCATCAGTCAAGTCCACCTCAAACAAGTGGAAATTCAGATTCTGCATCTGCCCAGTCCCCTTCTAGTTCTAGCGGAGAAGCCCAGAGTCGCACAGACCGAATTGTTTTTAAACTCTTCGGTAAAGAGCCAAATGAATTTCCTCTTGTACTTAGAGCACAGATTCTAGATTGGTTATCCCACAGTCCTACTGATATTGAGAGCTACATACGCCCTGGTTGTATTGTTTTGACCATTTATCTGCGTCAGGCCGAGGCTGTGTGGGAGGAACTTTGCTGTGATCTGACTTCCAGTTTGATTAAGCTGCTGGATGTCTCAGATGATACGTTTTGGAAAACTGGATGGGTTCATATCAGAGTGCAACATCAAATGGCATTCATTTTCAACGGTCAAGTTGTCATAGATACATCACTACCTTTCCGAAGCAACAATTATAGCAAAATTTGGACTGTTAGTCCAATTGCTGTACCGGCATCAAAGAGAGCTCAATTTTCTGTTAAAGGTGTCAACCTGATGCGCCCTGCCACAAGGTTAATGTGTGCTTTAGAAGGAAAATATCTGGTCTGTGAAGATGCTCATGAGTCAACGGATCAATACTCCGAGGAGCTTGATGAACTTCAGTGCATCCAATTTTCATGTTCTGTACCTGTGACAAATGGAAGAGGATTTATTGAGATTGAGGACCAGGGTTTGAGTAGCAGCTTCTTTCCTTTCATAGTGGCAGAAGAGGATGTTTGCTCAGAGATCCGCGTGCTTGAACCTCTACTAGAATCAAGTGAAACTGATCCAGATATTGAAGGGACTGGAAAAATTAAAGCCAAAAGTCAAGCTATGGATTTCATTCATGAAATGGGCTGGCTTCTTCACAGAAGCCAATTGAAATATAGGATGGTTAACTTGAACTCTGGTGTAGATCTCTTTCCATTAGAACGGTTCACATGGCTTATGGAGTTTTCAATGGACCATGATTGGTGTGCAGTGGTGAAAAAACTGTTGAACCTACTCTTTGATGAAACTGTTAATAAAGGAGATCACCCTACATTGTATCAAGCACTTTCAGAGACGGGTCTTCTTCACAGAGCTGTAAGGAGAAATAGCAAACAGTTAGTGGAGCTGCTTTTGCGATATGTTCCTGACAATACATCTGATGAACTAGGACCCAAAGACAAGGCGCTTGTTGGTGGAAAGAATCATAGCTACTTGTTTAGACCTGATGCTGTTGGTCCTGCCGGTTTGACACCACTTCATATAGCAGCGGGAAAAGATGGTTCCGAGGATGTACTGGATGCTCTGACTAATGATCCTTGCATGGTGGGAATTGAGGCATGGAAGAATGCCCGTGACAGTACAGGCTCCACACCTGAGGATTATGCGCGATTACGTGGCCATTATACTTACATTCACTTAGTGCAGAAAAAAATTAACAAAACACAAGGAGCAGCTCATGTGGTGGTTGAGATTCCCATCAATACGACAGAGAGCAATAAAACCCCGAAGCAAAATGAGTCATTCACTAGCTTAGAGATTGGAAAAGCTGAAGTAAGACGTAGCCGAGGGAACTGTAAACTTTGTGATACCAAAATTTCTTGTAGAACTGCAGTAGGAAGGTCTATGGTATACAGACCTGCAATGCTCTCTATGGTGGCCATAGCAGCGGTCTGTGTTTGTGTGGCTCTTCTGTTTAAGAGCTCACCCGAAGTTCTATACATGTTCCGACCTTTCAGATGGGAATCATTGGAGTTCGGAACAAGCTAA
Protein sequence

>MsaT004240.1

MGERLGAENYHFYGVGGSSDLSGMGRRSREWNLNDWRWDGDLFIASRVNPVQAESLRVGQQFFPLRSGIPVVGGSSNTSSSCSEEGDLEKGNKEGDRKRRVIVLEDDGLNDEAGALSLNLAGHVSPAVERDGKKSRVAGGTSNRAVCQVEDCGADLSRGKDYHRRHKVCEMHSKASRALVGNAMQRFCQQCSRFHILQEFDEGKRSCRRRLAGHNKRRRKTNQEAVPNGSPTNDDQTSSYLLISLLKILSNMHSDRSDQPTDQDLLTHLLRSLASQNDEQGSKNLSNLLREQENLLREGGSSRNSGMVSALFSNGSQGSPTVITQHQTVSMNQMQQEMVHTHDVRTSDHQLISSIKPSISNSPPAYSEARDSSGQTKMNNFDLNDIYVDSDDGTEDLERLPVSTNLATSSVDYPWTQQDSHQSSPPQTSGNSDSASAQSPSSSSGEAQSRTDRIVFKLFGKEPNEFPLVLRAQILDWLSHSPTDIESYIRPGCIVLTIYLRQAEAVWEELCCDLTSSLIKLLDVSDDTFWKTGWVHIRVQHQMAFIFNGQVVIDTSLPFRSNNYSKIWTVSPIAVPASKRAQFSVKGVNLMRPATRLMCALEGKYLVCEDAHESTDQYSEELDELQCIQFSCSVPVTNGRGFIEIEDQGLSSSFFPFIVAEEDVCSEIRVLEPLLESSETDPDIEGTGKIKAKSQAMDFIHEMGWLLHRSQLKYRMVNLNSGVDLFPLERFTWLMEFSMDHDWCAVVKKLLNLLFDETVNKGDHPTLYQALSETGLLHRAVRRNSKQLVELLLRYVPDNTSDELGPKDKALVGGKNHSYLFRPDAVGPAGLTPLHIAAGKDGSEDVLDALTNDPCMVGIEAWKNARDSTGSTPEDYARLRGHYTYIHLVQKKINKTQGAAHVVVEIPINTTESNKTPKQNESFTSLEIGKAEVRRSRGNCKLCDTKISCRTAVGRSMVYRPAMLSMVAIAAVCVCVALLFKSSPEVLYMFRPFRWESLEFGTS*