AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004274

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004274 MtrunA17_Chr1g0194631 98.814 759 9 0 50 808 1 759 0.0 1567
MsaG004274 MtrunA17_Chr5g0401921 47.275 844 366 15 16 804 42 861 0.0 687
MsaG004274 MtrunA17_Chr7g0235081 57.224 353 133 8 462 806 4 346 6.96e-128 386
MsaG004274 MtrunA17_Chr7g0270001 64.000 275 91 4 541 807 25 299 5.29e-114 348
MsaG004274 MtrunA17_Chr7g0235091 37.631 287 155 5 50 335 1 264 1.55e-55 194
MsaG004274 MtrunA17_Chr7g0270011 53.659 123 52 1 179 301 70 187 2.02e-36 140
MsaG004274 MtrunA17_Chr7g0270011 67.273 55 17 1 441 495 231 284 3.02e-15 77.8
MsaG004274 MtrunA17_Chr2g0297191 39.516 124 73 1 654 775 1027 1150 2.36e-22 103
MsaG004274 MtrunA17_Chr1g0147121 36.800 125 77 2 657 779 890 1014 1.33e-20 97.8
MsaG004274 MtrunA17_Chr8g0347071 37.705 122 74 2 660 779 808 929 2.19e-20 97.1
MsaG004274 MtrunA17_Chr3g0126701 37.705 122 74 2 660 779 922 1043 6.69e-20 95.5
MsaG004274 MtrunA17_Chr7g0275611 36.170 141 76 3 660 798 823 951 1.43e-19 94.4
MsaG004274 MtrunA17_Chr7g0221611 40.336 119 68 2 658 773 904 1022 4.35e-19 92.8
MsaG004274 MtrunA17_Chr1g0201621 36.641 131 81 1 647 775 72 202 1.65e-18 89.4
MsaG004274 MtrunA17_Chr1g0201311 36.641 131 81 1 647 775 72 202 3.84e-18 88.6
MsaG004274 MtrunA17_Chr2g0305371 30.921 152 95 3 624 773 306 449 3.30e-16 82.4
MsaG004274 MtrunA17_Chr1g0149581 32.283 127 80 1 655 775 1517 1643 6.03e-16 82.8
MsaG004274 MtrunA17_Chr1g0149611 34.783 115 69 1 665 773 369 483 9.30e-16 81.3
MsaG004274 MtrunA17_Chr5g0405161 45.652 92 43 3 206 296 12 97 2.07e-15 76.3
MsaG004274 MtrunA17_Chr5g0405161 49.398 83 39 2 413 495 89 168 1.16e-14 74.3
MsaG004274 MtrunA17_Chr6g0477001 32.847 137 87 3 648 779 101 237 3.16e-14 75.1
MsaG004274 MtrunA17_Chr3g0125251 27.692 195 103 4 583 775 1284 1442 8.92e-13 72.8
MsaG004274 MtrunA17_Chr5g0403241 29.655 145 85 2 648 775 1341 1485 1.53e-12 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004274 AT4G02020.1 56.057 842 304 14 14 808 34 856 0.0 869
MsaG004274 AT4G02020.4 54.161 781 292 14 14 747 34 795 0.0 766
MsaG004274 AT4G02020.3 52.848 755 290 14 14 721 34 769 0.0 716
MsaG004274 AT4G02020.2 52.848 755 290 14 14 721 34 769 0.0 716
MsaG004274 AT2G23380.2 62.143 420 141 10 395 804 262 673 5.50e-166 496
MsaG004274 AT2G23380.2 60.000 65 26 0 234 298 30 94 3.28e-18 90.1
MsaG004274 AT2G23380.1 62.143 420 141 10 395 804 489 900 2.86e-164 499
MsaG004274 AT2G23380.1 39.748 317 160 6 3 298 15 321 1.50e-53 201
MsaG004274 AT1G02580.1 47.583 393 173 6 411 798 323 687 6.02e-119 375
MsaG004274 AT1G02580.1 30.000 330 188 10 1 301 1 316 2.31e-24 109
MsaG004274 AT1G02580.2 43.382 272 125 5 411 680 323 567 7.87e-66 230
MsaG004274 AT1G02580.2 30.000 330 188 10 1 301 1 316 2.65e-24 108
MsaG004274 AT5G42400.7 38.583 127 76 1 651 775 1241 1367 1.52e-22 104
MsaG004274 AT5G42400.2 38.583 127 76 1 651 775 1241 1367 1.52e-22 104
MsaG004274 AT5G42400.8 38.583 127 76 1 651 775 1241 1367 1.52e-22 104
MsaG004274 AT5G42400.5 38.583 127 76 1 651 775 1241 1367 1.52e-22 104
MsaG004274 AT5G42400.4 38.583 127 76 1 651 775 1241 1367 1.52e-22 104
MsaG004274 AT5G42400.1 38.583 127 76 1 651 775 1241 1367 1.52e-22 104
MsaG004274 AT5G42400.3 38.583 127 76 1 651 775 1241 1367 1.52e-22 104
MsaG004274 AT5G42400.6 38.583 127 76 1 651 775 1241 1367 1.52e-22 104
MsaG004274 AT2G31650.1 42.857 119 65 2 658 773 897 1015 5.67e-21 99.4
MsaG004274 AT4G27910.1 37.302 126 77 2 657 780 883 1008 1.10e-20 98.6
MsaG004274 AT4G30860.1 34.337 166 94 5 613 773 287 442 1.57e-20 96.3
MsaG004274 AT5G53430.1 36.800 125 77 2 657 779 899 1023 1.63e-19 94.7
MsaG004274 AT1G05830.2 41.176 119 67 2 658 773 918 1036 7.42e-19 92.4
MsaG004274 AT1G05830.4 41.176 119 67 2 658 773 918 1036 7.42e-19 92.4
MsaG004274 AT1G05830.1 41.176 119 67 2 658 773 918 1036 7.42e-19 92.4
MsaG004274 AT1G05830.3 41.176 119 67 2 658 773 918 1036 7.42e-19 92.4
MsaG004274 AT1G76710.4 36.154 130 81 1 648 775 78 207 2.85e-18 89.4
MsaG004274 AT1G76710.2 36.154 130 81 1 648 775 78 207 3.06e-18 89.4
MsaG004274 AT1G76710.1 36.154 130 81 1 648 775 78 207 3.06e-18 89.4
MsaG004274 AT1G76710.3 36.154 130 81 1 648 775 78 207 3.10e-18 89.4
MsaG004274 AT3G61740.2 34.677 124 78 2 660 780 840 963 1.16e-17 88.6
MsaG004274 AT3G61740.1 34.677 124 78 2 660 780 876 999 1.29e-17 88.6
MsaG004274 AT3G59960.3 32.558 129 82 3 656 779 108 236 1.55e-15 78.6
MsaG004274 AT3G59960.2 32.558 129 82 3 656 779 108 236 1.55e-15 78.6
MsaG004274 AT3G59960.4 32.558 129 82 3 656 779 108 236 1.55e-15 78.6
MsaG004274 AT3G59960.1 32.558 129 82 3 656 779 108 236 2.32e-15 78.6
MsaG004274 AT2G44150.1 32.558 129 82 3 656 779 113 241 1.12e-13 73.9
MsaG004274 AT1G77300.1 31.285 179 108 5 599 775 979 1144 8.47e-13 73.2
MsaG004274 AT1G77300.2 33.846 130 84 2 648 775 1015 1144 1.12e-12 72.8

Find 185 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGACAAACTTACCTTAA+TGG 0.162835 1:-67624757 None:intergenic
TTGGGGAAGTCTGATGTTTC+TGG 0.176477 1:+67631813 MsaT004274.1:CDS
AAGGATTGTTCTTGTCTTAA+TGG 0.220999 1:+67629322 MsaT004274.1:CDS
GTCTCATTTGTGACAGCTTT+TGG 0.239771 1:-67627376 None:intergenic
CAAGGAAGTTTAAATATTCT+TGG 0.253650 1:+67629200 MsaT004274.1:CDS
CGTCCTTTATGTAAATTCTA+TGG 0.261939 1:+67625183 MsaT004274.1:CDS
TATGACATTGCAGCTTCTTC+TGG 0.297074 1:-67625071 None:intergenic
TGGATGGGGAGCCTTCTTAA+AGG 0.301212 1:+67631833 MsaT004274.1:CDS
GAACGAGTTGATGGAATTCT+TGG 0.315350 1:+67627430 MsaT004274.1:CDS
ATAGCTAGGAACTTACTTTC+TGG 0.330151 1:+67628124 MsaT004274.1:CDS
TTGCCCTGGTTTCTGTCTCC+TGG 0.332763 1:-67625219 None:intergenic
GATTACCTGGATTTCTAAAC+TGG 0.334427 1:-67626050 None:intergenic
CGAGAGAATTCAATCCATTA+AGG 0.335586 1:+67624743 MsaT004274.1:CDS
TTACAAGAATCCTGAGAATC+AGG 0.335745 1:-67627283 None:intergenic
TTCACCTTTGCATAGCAGTT+GGG 0.335759 1:-67632344 None:intergenic
CTGCTGGTCACCCAACAATA+TGG 0.336522 1:+67629227 MsaT004274.1:CDS
TTCAATTCTTTCCTCAAGAA+TGG 0.355699 1:+67625158 MsaT004274.1:CDS
AGTATCAGAGAAAGTAATAT+TGG 0.369713 1:+67626223 MsaT004274.1:CDS
CACAGGTCTATTATTTCAGC+TGG 0.378581 1:+67628205 MsaT004274.1:CDS
GAATTCTTGGTTTCTCAGAT+TGG 0.379032 1:+67627443 MsaT004274.1:CDS
AAGCTGCAATGTCATACTTC+AGG 0.381738 1:+67625078 MsaT004274.1:CDS
CTCAGCAACCTCGGTCTAGA+TGG 0.384033 1:-67629163 None:intergenic
TTGCAACTGATTTATCTTGT+TGG 0.384750 1:-67624706 None:intergenic
GCCCATGGAGGTGCTTGATC+TGG 0.396645 1:-67632884 None:intergenic
CTCTTCTATGATTATTGCTA+TGG 0.397112 1:+67632860 MsaT004274.1:CDS
GGTTTCTGTCTCCTGGACCC+TGG 0.403366 1:-67625212 None:intergenic
CTATAGGTAATGTTGGTTGC+TGG 0.407659 1:+67632785 MsaT004274.1:intron
AATTGCTGATTCGTCTCTCT+TGG 0.412706 1:-67632924 None:intergenic
TGGAAATGTTTGGCAGAAAC+AGG 0.418632 1:+67627500 MsaT004274.1:CDS
AAGGTTCCCACATCATCATT+TGG 0.421371 1:-67624681 None:intergenic
ACTCAGAAAACTTGAGTAAA+CGG 0.424610 1:+67627212 MsaT004274.1:CDS
TAATAGCAAGAGCTTACATT+TGG 0.424724 1:+67627348 MsaT004274.1:CDS
TTGAAGGGAGTGGAAATGTT+TGG 0.426717 1:+67627490 MsaT004274.1:CDS
CAACAGTTTCGACAGACATC+TGG 0.427301 1:-67629922 None:intergenic
TATTTCAGCTGGTTCAATCA+TGG 0.427476 1:+67628216 MsaT004274.1:CDS
GAGTATACTGCATGCAAGAT+TGG 0.429208 1:-67629273 None:intergenic
AATTTCTAGTATGGCATTTG+AGG 0.432378 1:+67625973 MsaT004274.1:intron
CTCCGTCTGTGAAGAGTCCC+TGG 0.432892 1:-67625121 None:intergenic
AGAGTGAAAAGAAGACAGTT+TGG 0.433099 1:+67626575 MsaT004274.1:intron
GTTTCTGTCTCCTGGACCCT+GGG 0.433547 1:-67625211 None:intergenic
TGGTGATCAGTGCTACCTTC+AGG 0.436446 1:+67626627 MsaT004274.1:CDS
ATGTTGTTAGCAAGTATGTT+GGG 0.438742 1:+67626023 MsaT004274.1:CDS
CAGCTGTGGGGATGGTACAC+TGG 0.439264 1:+67631605 MsaT004274.1:intron
TCAATGGAAGAACGAGTTGA+TGG 0.443369 1:+67627421 MsaT004274.1:CDS
TTCTCTAGATACATGCTCAT+AGG 0.445332 1:-67626289 None:intergenic
CTTAATGGATTGAATTCTCT+CGG 0.445675 1:-67624742 None:intergenic
AAGATCGCTTCCTGATTCTC+AGG 0.449551 1:+67627273 MsaT004274.1:CDS
CACAATGGCAACCTCTGAAC+CGG 0.454848 1:-67629840 None:intergenic
ATTCACCTTTGCATAGCAGT+TGG 0.455961 1:-67632345 None:intergenic
GTTCGAAGAGCTGCAAGAAC+CGG 0.461634 1:+67629821 MsaT004274.1:CDS
ACTGCCAGGTTCTTCTGCAC+TGG 0.463190 1:-67627128 None:intergenic
TCGTCTCTCTTGGAACCCTC+AGG 0.464748 1:-67632914 None:intergenic
AGAGAAAGAGTTATACTTGA+AGG 0.465034 1:+67627474 MsaT004274.1:CDS
TACTAACTTACCACAAAATA+CGG 0.467763 1:-67625639 None:intergenic
ATTAATCATAACCTTTAAGA+AGG 0.469624 1:-67631844 None:intergenic
TGGATTGAATTCTCTCGGCC+TGG 0.469701 1:-67624737 None:intergenic
AAACCATAGAATTTACATAA+AGG 0.469948 1:-67625186 None:intergenic
ACCGGCATCAATGTGTTCCT+TGG 0.472051 1:-67632831 None:intergenic
AAATATTCTTGGAAGTCTGC+TGG 0.473416 1:+67629211 MsaT004274.1:CDS
ATTGGCCATGGCTCATCAAT+GGG 0.475159 1:-67632975 None:intergenic
GAAGTCTGATGTTTCTGGAT+GGG 0.479401 1:+67631818 MsaT004274.1:CDS
GAGCCACATCGGCGTGGAGA+AGG 0.489074 1:+67631630 MsaT004274.1:CDS
GATTACCACCATATACATCT+TGG 0.489282 1:+67625289 MsaT004274.1:CDS
TCGACTTCTACATTGACTCT+TGG 0.492456 1:-67629863 None:intergenic
TCTGTCGAAACTGTTGGGTT+AGG 0.492462 1:+67629929 MsaT004274.1:CDS
GTTCTTGATGCTTATCGTAA+AGG 0.493028 1:+67632287 MsaT004274.1:CDS
AAGTCTGATGTTTCTGGATG+GGG 0.494803 1:+67631819 MsaT004274.1:CDS
GCTGACGATCAATCAGTTGT+TGG 0.496379 1:+67625502 MsaT004274.1:CDS
GGGCACCAGTTTAGAAATCC+AGG 0.497692 1:+67626045 MsaT004274.1:CDS
TCACCTTTGCATAGCAGTTG+GGG 0.500009 1:-67632343 None:intergenic
AGAAAGTAATATTGGTAGGT+TGG 0.500552 1:+67626231 MsaT004274.1:CDS
AATGTTGTTAGCAAGTATGT+TGG 0.504453 1:+67626022 MsaT004274.1:CDS
AGATGTCTGTCGAAACTGTT+GGG 0.506928 1:+67629924 MsaT004274.1:CDS
AGCAGTTGGGGTTTGATGAG+TGG 0.507871 1:-67632331 None:intergenic
CCATGGCTCATCAATGGGAT+TGG 0.508976 1:-67632970 None:intergenic
GACGACAGAAAAGGTTATCA+AGG 0.510059 1:-67626336 None:intergenic
AGAGTTATACTTGAAGGGAG+TGG 0.511021 1:+67627480 MsaT004274.1:CDS
GACCAGCATCCTAAAAGCTC+AGG 0.512493 1:+67626253 MsaT004274.1:CDS
AGCAAGAGCTTACATTTGGA+TGG 0.514477 1:+67627352 MsaT004274.1:CDS
GTCCAGATCAAGCACCTCCA+TGG 0.515220 1:+67632882 MsaT004274.1:CDS
TTCCTTACGGGATATATCAA+AGG 0.515656 1:-67626483 None:intergenic
CCAGGCACCGACGACAGAAA+AGG 0.520888 1:-67626345 None:intergenic
CCTGCTCATTCAAATCGAAA+AGG 0.520942 1:-67632037 None:intergenic
TTTCTTATCCCGAAAAGACC+CGG 0.523094 1:-67627065 None:intergenic
GGAAGTCTGATGTTTCTGGA+TGG 0.525818 1:+67631817 MsaT004274.1:CDS
TGTTTCTTTGCTCTACCTTG+TGG 0.528560 1:-67632947 None:intergenic
GATTGTTCTTGTCTTAATGG+TGG 0.531062 1:+67629325 MsaT004274.1:CDS
CTGATTTATCTTGTTGGACA+AGG 0.531637 1:-67624700 None:intergenic
AGCTGAAATAATAGACCTGT+GGG 0.532398 1:-67628203 None:intergenic
AAGCATCAAGATTCCACGTA+TGG 0.533307 1:+67625263 MsaT004274.1:CDS
CCATGACAATCAATTCTTGT+CGG 0.533836 1:-67627166 None:intergenic
TCGGTCTAGATGGCATGTCT+TGG 0.533864 1:-67629153 None:intergenic
TCTGCAGTTGCCTCATAGCT+AGG 0.536215 1:+67628110 MsaT004274.1:intron
CCATCCAGTGCAGAAGAACC+TGG 0.536754 1:+67627124 MsaT004274.1:CDS
AAGTATAACTCTTTCTCTAG+AGG 0.540229 1:-67627469 None:intergenic
TGAGGAACACGGGTTAAATG+AGG 0.542454 1:+67625991 MsaT004274.1:CDS
TTCTGGTTCGAAGACTTGCA+TGG 0.543056 1:+67628141 MsaT004274.1:CDS
CCAGGTTCTTCTGCACTGGA+TGG 0.543278 1:-67627124 None:intergenic
GAGAAAGAGTTATACTTGAA+GGG 0.544561 1:+67627475 MsaT004274.1:CDS
TCACGCCCAGCAGCAAAACA+TGG 0.547646 1:-67629892 None:intergenic
AGTGTAGTACTCTGACTGCC+AGG 0.547776 1:-67627142 None:intergenic
CCCTCAGGTTTACGAGCCCA+TGG 0.549721 1:-67632899 None:intergenic
TCAGAGAAAGTAATATTGGT+AGG 0.550783 1:+67626227 MsaT004274.1:CDS
TACATTGACTCTTGGCACAA+TGG 0.551079 1:-67629855 None:intergenic
GTTCACCCGTGTACTCTCCT+AGG 0.552754 1:-67631953 None:intergenic
CAGATGTCTGTCGAAACTGT+TGG 0.555785 1:+67629923 MsaT004274.1:CDS
AGCTGTGGGGATGGTACACT+GGG 0.558478 1:+67631606 MsaT004274.1:intron
ATGACATGTCTTGATTGCCC+TGG 0.559314 1:-67625233 None:intergenic
ATCATAGAAGAGCTCCTCAC+CGG 0.561900 1:-67632849 None:intergenic
TTCTTATCCCGAAAAGACCC+GGG 0.566631 1:-67627064 None:intergenic
GGGTCCAGGAGACAGAAACC+AGG 0.568933 1:+67625215 MsaT004274.1:CDS
GCCAAGGAACACATTGATGC+CGG 0.569010 1:+67632830 MsaT004274.1:CDS
ACTGCTGCTATAGGTAATGT+TGG 0.569757 1:+67632778 MsaT004274.1:intron
TCAGTTGCAAAAGCAAATCC+AGG 0.571291 1:+67624719 MsaT004274.1:CDS
ACGCATCAGGAATCAGAGAA+TGG 0.572672 1:+67625480 MsaT004274.1:intron
CCGACAAGAATTGATTGTCA+TGG 0.573856 1:+67627166 MsaT004274.1:CDS
TAATGTCAGCAGTATCAACC+AGG 0.580659 1:+67625103 MsaT004274.1:CDS
AAAGTAAGTTCCTAGCTATG+AGG 0.582475 1:-67628120 None:intergenic
AGAGCCGCCAAATGATGATG+TGG 0.582841 1:+67624674 MsaT004274.1:CDS
AGCTGCAAGAACCGGTTCAG+AGG 0.582865 1:+67629829 MsaT004274.1:CDS
GTTGCTGGAGATCATCGAGT+TGG 0.583554 1:+67632800 MsaT004274.1:CDS
CACATTGATTGACACCCACA+TGG 0.585645 1:-67629295 None:intergenic
TAAAGGACGGTCCATTCTTG+AGG 0.587453 1:-67625169 None:intergenic
ACAATTGTAGAGGAGACAGA+TGG 0.588503 1:+67627091 MsaT004274.1:CDS
TCCAGATCAAGCACCTCCAT+GGG 0.593087 1:+67632883 MsaT004274.1:CDS
TGACCTTCTCCACGCCGATG+TGG 0.594534 1:-67631633 None:intergenic
CACAGAGAAGCTGATAAGCG+TGG 0.596923 1:+67631984 MsaT004274.1:CDS
TGAGTCACATTGTGCCGTGT+CGG 0.596974 1:-67627251 None:intergenic
TCTTTGTACAGCTGTGGGGA+TGG 0.597302 1:+67631597 MsaT004274.1:intron
AATCAGTTGTTGGAAGAAGA+CGG 0.597314 1:+67625512 MsaT004274.1:CDS
TAAGAAAACTACAATTGTAG+AGG 0.600144 1:+67627081 MsaT004274.1:CDS
CACGCCCAGCAGCAAAACAT+GGG 0.606423 1:-67629891 None:intergenic
ATATCTAGTTATATGCATGA+TGG 0.607787 1:+67628166 MsaT004274.1:CDS
AACCAGGGACTCTTCACAGA+CGG 0.608975 1:+67625119 MsaT004274.1:CDS
GTTCCCACATCATCATTTGG+CGG 0.610279 1:-67624678 None:intergenic
AAAATTTGACACAGAGTGCA+CGG 0.611174 1:+67628249 MsaT004274.1:CDS
TTTCAAAAGATTCAACTCCC+GGG 0.612464 1:+67627047 MsaT004274.1:CDS
GGAACACGGGTTAAATGAGG+AGG 0.618859 1:+67625994 MsaT004274.1:CDS
ATATGGAAAAGAATTGCTGA+TGG 0.619252 1:+67629244 MsaT004274.1:CDS
GAGGCATGTCAATTCCCCAC+AGG 0.619873 1:+67628188 MsaT004274.1:CDS
CAGCTGAAATAATAGACCTG+TGG 0.621680 1:-67628204 None:intergenic
AGTATGGCATTTGAGGAACA+CGG 0.622172 1:+67625980 MsaT004274.1:intron
ACAGAGAAGCTGATAAGCGT+GGG 0.622981 1:+67631985 MsaT004274.1:CDS
TCCATGGGCTCGTAAACCTG+AGG 0.626427 1:+67632898 MsaT004274.1:CDS
AATATCCAAGATGTATATGG+TGG 0.626743 1:-67625294 None:intergenic
AAACCCCAACTGCTATGCAA+AGG 0.629398 1:+67632340 MsaT004274.1:CDS
CCATGGGCTCGTAAACCTGA+GGG 0.632587 1:+67632899 MsaT004274.1:CDS
TCGAGTTGGCATATTCGCCA+AGG 0.633128 1:+67632814 MsaT004274.1:CDS
CATAGAATTTACATAAAGGA+CGG 0.634365 1:-67625182 None:intergenic
GAGCCGCCAAATGATGATGT+GGG 0.634443 1:+67624675 MsaT004274.1:CDS
GGGAATCTAATGCATCACTC+AGG 0.636717 1:-67626315 None:intergenic
GGTTCAATCATGGACGACAA+AGG 0.638496 1:+67628226 MsaT004274.1:CDS
TTATGATCAACGTGGAAGTG+AGG 0.638890 1:+67625540 MsaT004274.1:CDS
CAATTGTAGAGGAGACAGAT+GGG 0.639184 1:+67627092 MsaT004274.1:CDS
ACAGTTTGGTCTGAGCCTGT+AGG 0.639905 1:+67626589 MsaT004274.1:CDS
GGTGGTAATCTTTCCATACG+TGG 0.640705 1:-67625276 None:intergenic
TGATTACCTAGGAGAGTACA+CGG 0.641342 1:+67631947 MsaT004274.1:CDS
ACGACAGAAAAGGTTATCAA+GGG 0.642705 1:-67626335 None:intergenic
TCTAGTTATATGCATGATGG+AGG 0.645067 1:+67628169 MsaT004274.1:CDS
GTATGGCATTTGAGGAACAC+GGG 0.649134 1:+67625981 MsaT004274.1:intron
TGTTGTTAGCAAGTATGTTG+GGG 0.651009 1:+67626024 MsaT004274.1:CDS
GGAACACATTGATGCCGGTG+AGG 0.653810 1:+67632835 MsaT004274.1:CDS
CTGGGAGAGCCACATCGGCG+TGG 0.657062 1:+67631624 MsaT004274.1:CDS
GCATGCAGTATACTCCATGT+GGG 0.660903 1:+67629281 MsaT004274.1:CDS
GTGTCAATCAATGTGTGGAA+AGG 0.662829 1:+67629303 MsaT004274.1:CDS
GGTCCAGGAGACAGAAACCA+GGG 0.668116 1:+67625216 MsaT004274.1:CDS
GACGAATCAGCAATTCCACA+AGG 0.669739 1:+67632932 MsaT004274.1:CDS
TGCATGCAGTATACTCCATG+TGG 0.671233 1:+67629280 MsaT004274.1:CDS
GTTGTTAGCAAGTATGTTGG+GGG 0.673475 1:+67626025 MsaT004274.1:CDS
ATTGATATGCAGTGACAGTG+AGG 0.679098 1:+67625564 MsaT004274.1:CDS
CAGGGACTCTTCACAGACGG+AGG 0.683082 1:+67625122 MsaT004274.1:CDS
ACACTCTTTGTACAGCTGTG+GGG 0.688643 1:+67631593 MsaT004274.1:intron
CGGATCTATTATGATCAACG+TGG 0.691963 1:+67625532 MsaT004274.1:CDS
TGTGGGTGTCAATCAATGTG+TGG 0.694307 1:+67629298 MsaT004274.1:CDS
AGGTAGCACTGATCACCACA+TGG 0.697472 1:-67626622 None:intergenic
GTACACTGGGAGAGCCACAT+CGG 0.707893 1:+67631619 MsaT004274.1:CDS
AGTCACAAATCAGTCCGACA+CGG 0.716243 1:+67627237 MsaT004274.1:CDS
GAAGAATTAACAGAACCTGA+CGG 0.721323 1:+67625586 MsaT004274.1:CDS
AATGTCAGCAGTATCAACCA+GGG 0.730778 1:+67625104 MsaT004274.1:CDS
AAGACATGCCATCTAGACCG+AGG 0.737982 1:+67629155 MsaT004274.1:CDS
CGGCGTGGAGAAGGTCAATG+CGG 0.745375 1:+67631639 MsaT004274.1:CDS
TCAGGTTTACGAGCCCATGG+AGG 0.763936 1:-67632896 None:intergenic
GATTACCTAGGAGAGTACAC+GGG 0.774788 1:+67631948 MsaT004274.1:CDS
GCTGAAATAATAGACCTGTG+GGG 0.791200 1:-67628202 None:intergenic
GTAGGTGATAGAAAACCATG+TGG 0.821461 1:+67626607 MsaT004274.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 67624472 67632982 67624472 ID=MsaG004274
Chr1 mRNA 67624472 67632982 67624472 ID=MsaT004274.1;Parent=MsaG004274
Chr1 exon 67624472 67624474 67624472 ID=MsaT004274.1.exon1;Parent=MsaT004274.1
Chr1 CDS 67624472 67624474 67624472 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67624672 67624764 67624672 ID=MsaT004274.1.exon2;Parent=MsaT004274.1
Chr1 CDS 67624672 67624764 67624672 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67625054 67625325 67625054 ID=MsaT004274.1.exon3;Parent=MsaT004274.1
Chr1 CDS 67625054 67625325 67625054 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67625489 67625650 67625489 ID=MsaT004274.1.exon4;Parent=MsaT004274.1
Chr1 CDS 67625489 67625650 67625489 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67625982 67626066 67625982 ID=MsaT004274.1.exon5;Parent=MsaT004274.1
Chr1 CDS 67625982 67626066 67625982 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67626211 67626366 67626211 ID=MsaT004274.1.exon6;Parent=MsaT004274.1
Chr1 CDS 67626211 67626366 67626211 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67626450 67626497 67626450 ID=MsaT004274.1.exon7;Parent=MsaT004274.1
Chr1 CDS 67626450 67626497 67626450 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67626577 67626648 67626577 ID=MsaT004274.1.exon8;Parent=MsaT004274.1
Chr1 CDS 67626577 67626648 67626577 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67627028 67627521 67627028 ID=MsaT004274.1.exon9;Parent=MsaT004274.1
Chr1 CDS 67627028 67627521 67627028 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67628117 67628270 67628117 ID=MsaT004274.1.exon10;Parent=MsaT004274.1
Chr1 CDS 67628117 67628270 67628117 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67629151 67629371 67629151 ID=MsaT004274.1.exon11;Parent=MsaT004274.1
Chr1 CDS 67629151 67629371 67629151 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67629819 67629950 67629819 ID=MsaT004274.1.exon12;Parent=MsaT004274.1
Chr1 CDS 67629819 67629950 67629819 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67631608 67631695 67631608 ID=MsaT004274.1.exon13;Parent=MsaT004274.1
Chr1 CDS 67631608 67631695 67631608 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67631807 67631854 67631807 ID=MsaT004274.1.exon14;Parent=MsaT004274.1
Chr1 CDS 67631807 67631854 67631807 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67631930 67632058 67631930 ID=MsaT004274.1.exon15;Parent=MsaT004274.1
Chr1 CDS 67631930 67632058 67631930 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67632284 67632361 67632284 ID=MsaT004274.1.exon16;Parent=MsaT004274.1
Chr1 CDS 67632284 67632361 67632284 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Chr1 exon 67632791 67632982 67632791 ID=MsaT004274.1.exon17;Parent=MsaT004274.1
Chr1 CDS 67632791 67632982 67632791 ID=cds.MsaT004274.1;Parent=MsaT004274.1
Gene Sequence

>MsaT004274.1

ATGGAAGAGCCGCCAAATGATGATGTGGGAACCTTGTCCAACAAGATAAATCAGTTGCAAAAGCAAATCCAGGCCGAGAGAATTCAATCCATTAAGGAAAAACTTCAGAGGAACCAGAAGAAGCTGCAATGTCATACTTCAGGAATAATGTCAGCAGTATCAACCAGGGACTCTTCACAGACGGAGGAAAATACGACACGTTCAATTCTTTCCTCAAGAATGGACCGTCCTTTATGTAAATTCTATGGTTTTACCCAGGGTCCAGGAGACAGAAACCAGGGCAATCAAGACATGTCATCTGCTACAAGCATCAAGATTCCACGTATGGAAAGATTACCACCATATACATCTTGGATATTTTTGGACAGGAATCAGAGAATGGCTGACGATCAATCAGTTGTTGGAAGAAGACGGATCTATTATGATCAACGTGGAAGTGAGGCATTGATATGCAGTGACAGTGAGGAAGAATTAACAGAACCTGACGGTGAAAAACATGAATTTTGTGATGCTGAAGACCGTATTTTGTGTATGGCATTTGAGGAACACGGGTTAAATGAGGAGGTTTTGAATGTTGTTAGCAAGTATGTTGGGGGCACCAGTTTAGAAATCCAGGAGAGGTACAAAAGTATCAGAGAAAGTAATATTGGTAGGTTGGACCAGCATCCTAAAAGCTCAGGAGAACATGACTTTCCTATGAGCATGTATCTAGAGAAAAACCTGAGTGATGCATTAGATTCCCTTGATAACCTTTTCTGTCGTCGGTGCCTGATTTTTGACTGTCGACTGCATGGCTGTTCTCAACCTTTGATATATCCCAGTGAAAAGAAGACAGTTTGGTCTGAGCCTGTAGGTGATAGAAAACCATGTGGTGATCAGTGCTACCTTCAGTTAAAGGTTGTTAAAAGTTTTTCAAAAGATTCAACTCCCGGGTCTTTTCGGGATAAGAAAACTACAATTGTAGAGGAGACAGATGGGATATTGTCACCATCCAGTGCAGAAGAACCTGGCAGTCAGAGTACTACACTTCCGACAAGAATTGATTGTCATGGATATATCAATTTAAATGACCCTGACTCAGAAAACTTGAGTAAACGGAAAGTCACAAATCAGTCCGACACGGCACAATGTGACTCAAGATCGCTTCCTGATTCTCAGGATTCTTGTAAAAAGCTGAAGAGAATCTCAGATGTAGTTACTGTAATTACTGATAATAGCAAGAGCTTACATTTGGATGGACCAAAAGCTGTCACAAATGAGACAGAACTTAAAAATTCGTTGAATTCAATGGAAGAACGAGTTGATGGAATTCTTGGTTTCTCAGATTGGAAACCTCTAGAGAAAGAGTTATACTTGAAGGGAGTGGAAATGTTTGGCAGAAACAGTTGCCTCATAGCTAGGAACTTACTTTCTGGTTCGAAGACTTGCATGGAAATATCTAGTTATATGCATGATGGAGGCATGTCAATTCCCCACAGGTCTATTATTTCAGCTGGTTCAATCATGGACGACAAAGGAAAATTTGACACAGAGTGCACGGACCAAGACATGCCATCTAGACCGAGGTTGCTGAGAAAAAGAGGCAAAACAAGGAAGTTTAAATATTCTTGGAAGTCTGCTGGTCACCCAACAATATGGAAAAGAATTGCTGATGGCAAAAACCAATCTTGCATGCAGTATACTCCATGTGGGTGTCAATCAATGTGTGGAAAGGATTGTTCTTGTCTTAATGGTGGAACATGCTGTGAAAAATATTGTGGTTGTTCGAAGAGCTGCAAGAACCGGTTCAGAGGTTGCCATTGTGCCAAGAGTCAATGTAGAAGTCGACAATGCCCATGTTTTGCTGCTGGGCGTGAATGTGATCCAGATGTCTGTCGAAACTGTTGGGTTAGCTGTGGGGATGGTACACTGGGAGAGCCACATCGGCGTGGAGAAGGTCAATGCGGCAATATGAGACTTCTTTTAAGGCAACAACAGAGGATTATCTTGGGGAAGTCTGATGTTTCTGGATGGGGAGCCTTCTTAAAGAATCCTGTTAACAAAAATGATTACCTAGGAGAGTACACGGGTGAACTGATCTCACACAGAGAAGCTGATAAGCGTGGGAAAATATATGATCGTGCGAATTCATCTTTCCTTTTCGATTTGAATGAGCAGTATGTTCTTGATGCTTATCGTAAAGGAGACAAGTTGAAATTTGCAAACCACTCATCAAACCCCAACTGCTATGCAAAGGTAATGTTGGTTGCTGGAGATCATCGAGTTGGCATATTCGCCAAGGAACACATTGATGCCGGTGAGGAGCTCTTCTATGATTATTGCTATGGTCCAGATCAAGCACCTCCATGGGCTCGTAAACCTGAGGGTTCCAAGAGAGACGAATCAGCAATTCCACAAGGTAGAGCAAAGAAACACCAATCCCATTGA
Protein sequence

>MsaT004274.1

MEEPPNDDVGTLSNKINQLQKQIQAERIQSIKEKLQRNQKKLQCHTSGIMSAVSTRDSSQTEENTTRSILSSRMDRPLCKFYGFTQGPGDRNQGNQDMSSATSIKIPRMERLPPYTSWIFLDRNQRMADDQSVVGRRRIYYDQRGSEALICSDSEEELTEPDGEKHEFCDAEDRILCMAFEEHGLNEEVLNVVSKYVGGTSLEIQERYKSIRESNIGRLDQHPKSSGEHDFPMSMYLEKNLSDALDSLDNLFCRRCLIFDCRLHGCSQPLIYPSEKKTVWSEPVGDRKPCGDQCYLQLKVVKSFSKDSTPGSFRDKKTTIVEETDGILSPSSAEEPGSQSTTLPTRIDCHGYINLNDPDSENLSKRKVTNQSDTAQCDSRSLPDSQDSCKKLKRISDVVTVITDNSKSLHLDGPKAVTNETELKNSLNSMEERVDGILGFSDWKPLEKELYLKGVEMFGRNSCLIARNLLSGSKTCMEISSYMHDGGMSIPHRSIISAGSIMDDKGKFDTECTDQDMPSRPRLLRKRGKTRKFKYSWKSAGHPTIWKRIADGKNQSCMQYTPCGCQSMCGKDCSCLNGGTCCEKYCGCSKSCKNRFRGCHCAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGTLGEPHRRGEGQCGNMRLLLRQQQRIILGKSDVSGWGAFLKNPVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNEQYVLDAYRKGDKLKFANHSSNPNCYAKVMLVAGDHRVGIFAKEHIDAGEELFYDYCYGPDQAPPWARKPEGSKRDESAIPQGRAKKHQSH*