AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004284

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004284 MtrunA17_Chr1g0194741 96.472 652 20 2 1 649 1 652 0.0 1285
MsaG004284 MtrunA17_Chr1g0197591 61.504 1104 352 19 10 1098 3 1048 0.0 1233
MsaG004284 MtrunA17_Chr1g0194731 95.415 458 18 3 649 1104 1 457 0.0 828
MsaG004284 MtrunA17_Chr8g0371601 28.947 570 351 17 129 674 196 735 1.22e-43 173
MsaG004284 MtrunA17_Chr8g0371601 31.658 199 111 7 911 1086 957 1153 1.32e-16 85.5
MsaG004284 MtrunA17_Chr5g0405701 33.690 374 228 9 303 674 418 773 7.57e-42 167
MsaG004284 MtrunA17_Chr5g0405701 34.483 145 71 4 965 1086 1040 1183 3.19e-16 84.3
MsaG004284 MtrunA17_Chr8g0343551 66.667 78 26 0 1025 1102 12 89 1.59e-31 118
MsaG004284 MtrunA17_Chr8g0377481 28.079 406 223 12 383 770 381 735 3.11e-30 129
MsaG004284 MtrunA17_Chr8g0377481 32.847 137 68 6 972 1095 838 963 1.69e-13 75.5
MsaG004284 MtrunA17_Chr3g0122391 28.027 446 255 17 272 678 305 723 1.15e-29 127
MsaG004284 MtrunA17_Chr3g0122391 32.919 161 82 8 947 1095 874 1020 3.94e-14 77.4
MsaG004284 MtrunA17_Chr5g0447641 25.833 360 215 11 351 685 576 908 2.88e-23 107
MsaG004284 MtrunA17_Chr5g0447641 33.333 147 60 7 975 1095 1074 1208 6.63e-12 70.1
MsaG004284 MtrunA17_Chr8g0392301 26.075 372 221 9 351 684 340 695 3.13e-22 103
MsaG004284 MtrunA17_Chr8g0392301 33.333 150 75 5 963 1095 849 990 2.17e-14 78.2
MsaG004284 MtrunA17_Chr3g0136601 29.464 336 180 14 367 669 335 646 9.61e-20 95.5
MsaG004284 MtrunA17_Chr4g0013831 27.273 352 192 11 378 708 324 632 2.92e-18 90.5
MsaG004284 MtrunA17_Chr1g0150511 26.027 365 203 12 399 713 381 728 7.01e-18 89.7
MsaG004284 MtrunA17_Chr7g0269631 27.459 244 137 7 454 690 393 603 7.21e-16 82.4
MsaG004284 MtrunA17_Chr8g0364781 37.037 135 62 6 978 1102 15 136 1.25e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004284 AT2G47430.1 38.024 1144 614 25 11 1096 12 1118 0.0 678
MsaG004284 AT2G17820.1 27.872 592 382 15 103 674 196 762 2.48e-44 176
MsaG004284 AT2G17820.1 31.658 199 97 7 926 1095 1004 1192 3.57e-16 84.7
MsaG004284 AT1G27320.1 26.316 532 297 19 272 769 315 785 9.74e-31 131
MsaG004284 AT1G27320.1 29.221 154 75 5 972 1101 887 1030 1.62e-11 69.3
MsaG004284 AT2G01830.2 27.869 366 220 11 338 677 415 762 2.49e-28 124
MsaG004284 AT2G01830.2 36.567 134 67 4 972 1095 942 1067 2.46e-15 82.0
MsaG004284 AT2G01830.1 27.869 366 220 11 338 677 392 739 2.55e-28 124
MsaG004284 AT2G01830.1 36.567 134 67 4 972 1095 919 1044 2.37e-15 82.0
MsaG004284 AT2G01830.4 27.869 366 220 11 338 677 392 739 2.55e-28 124
MsaG004284 AT2G01830.4 36.567 134 67 4 972 1095 919 1044 2.37e-15 82.0
MsaG004284 AT2G01830.6 27.869 366 220 11 338 677 392 739 2.55e-28 124
MsaG004284 AT2G01830.6 36.567 134 67 4 972 1095 919 1044 2.37e-15 82.0
MsaG004284 AT2G01830.3 27.869 366 220 11 338 677 392 739 2.55e-28 124
MsaG004284 AT2G01830.3 36.567 134 67 4 972 1095 919 1044 2.37e-15 82.0
MsaG004284 AT2G01830.5 27.869 366 220 11 338 677 404 751 2.71e-28 124
MsaG004284 AT2G01830.5 36.567 134 67 4 972 1095 931 1056 2.45e-15 82.0
MsaG004284 AT5G35750.1 28.916 332 173 12 380 680 573 872 4.95e-25 113
MsaG004284 AT1G66340.1 27.532 316 178 9 378 686 323 594 4.81e-19 93.6
MsaG004284 AT5G10720.1 30.037 273 154 11 399 669 371 608 3.24e-18 91.3
MsaG004284 AT2G40940.1 25.538 325 185 9 364 676 309 588 2.38e-15 81.3

Find 205 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTATGATTATATCTTAA+TGG 0.123404 1:-67791518 MsaT004284.1:CDS
GCTTATCTTGCAGGCCTTAT+TGG 0.151594 1:-67794790 MsaT004284.1:CDS
GATAGTGTTGTTAGATATTA+TGG 0.179176 1:+67796191 None:intergenic
AGGTGACATTAGTAGAATTA+AGG 0.196080 1:+67796716 None:intergenic
TGTGATCTAGGTTCATTTCT+TGG 0.224149 1:+67791850 None:intergenic
ATTGATGCAAGTTGGATAAA+TGG 0.240438 1:-67796165 MsaT004284.1:CDS
AGTTGTTACTAATTCAAAAT+TGG 0.240702 1:-67794743 MsaT004284.1:CDS
GTTTCAATTCAAGCTGTTAA+TGG 0.241296 1:-67795889 MsaT004284.1:CDS
GATGGAAAAGTTATTGTAAA+AGG 0.243895 1:-67795949 MsaT004284.1:CDS
TTGCTACCCCATTGATATAA+TGG 0.245070 1:-67795770 MsaT004284.1:CDS
TCAAGGGACAAAATCATATT+TGG 0.248361 1:-67795980 MsaT004284.1:CDS
TTCTGAACTTGGTTTCAAAT+TGG 0.254047 1:+67794717 None:intergenic
ACTGACTTGTGATTCTCTTT+AGG 0.274643 1:+67794003 None:intergenic
TTCACATGAATTTGCATCAT+TGG 0.275818 1:-67796130 MsaT004284.1:CDS
CACAGGTTGGATATAGTATA+TGG 0.279878 1:+67796245 None:intergenic
AGCAAAAGCAAGACTCTTAT+TGG 0.282452 1:+67794834 None:intergenic
GATCTTCGTGCTTATCTTGC+AGG 0.288028 1:-67794799 MsaT004284.1:CDS
ATTAGTAACAACTAATTTAG+AGG 0.290279 1:+67794753 None:intergenic
ACAAAAGTCCTATGTTAATC+TGG 0.291733 1:-67791770 MsaT004284.1:CDS
TTCTTTCCACTTAATGGTTT+AGG 0.294905 1:+67791691 None:intergenic
AGTAAATGCTCTTTCTTGAT+AGG 0.295947 1:+67791229 None:intergenic
TTCCTGAGTTCGGTGGTAAT+TGG 0.302430 1:-67791891 MsaT004284.1:CDS
TTTGAAACCAAGTTCAGAAT+TGG 0.304813 1:-67794713 MsaT004284.1:CDS
GTGTTAGCTGTCCCACAAAA+TGG 0.319563 1:-67795045 MsaT004284.1:CDS
TTCATCTTTCATGATCTTGC+TGG 0.339861 1:-67797108 MsaT004284.1:intron
ATGATTTACCTAGCAAATCT+TGG 0.341823 1:+67794659 None:intergenic
TTGGAAGGTACTTGTGATCT+AGG 0.351381 1:+67791838 None:intergenic
GCTGTTAAATTTACTGATGA+AGG 0.355175 1:-67794228 MsaT004284.1:CDS
GGTGACATTAGTAGAATTAA+GGG 0.356467 1:+67796717 None:intergenic
ATTCAAGCTGTTAATGGTAA+TGG 0.357123 1:-67795883 MsaT004284.1:CDS
TTCTATTCCCATTATATCAA+TGG 0.357816 1:+67795762 None:intergenic
GTAGATTGTTCCAAGTCATT+AGG 0.359195 1:-67791918 MsaT004284.1:CDS
TCCAACCAAACAACTTTACT+AGG 0.359940 1:+67792018 None:intergenic
GTGATATCAAAACCATTTCA+TGG 0.361788 1:-67791943 MsaT004284.1:CDS
TGCTACCCCATTGATATAAT+GGG 0.365131 1:-67795769 MsaT004284.1:CDS
CCAACAAGATCCATGTCTTA+TGG 0.374116 1:-67794064 MsaT004284.1:CDS
ACAATTCGTACTGCTAGTTC+TGG 0.375945 1:-67792495 MsaT004284.1:CDS
TCTATTCCCATTATATCAAT+GGG 0.382599 1:+67795763 None:intergenic
ATGCTCTTTCTTGATAGGTT+TGG 0.386579 1:+67791234 None:intergenic
ACACCTTTCTTCATTGCCAT+TGG 0.389100 1:+67794354 None:intergenic
GAGACAGTGAATTATGAGTA+TGG 0.390792 1:-67792561 MsaT004284.1:CDS
TCACATGAATTTGCATCATT+GGG 0.392411 1:-67796129 MsaT004284.1:CDS
GTATTTGAAGTGGATGATAC+AGG 0.399459 1:-67794036 MsaT004284.1:CDS
TCAATCCTAGTAAAGTTGTT+TGG 0.399703 1:-67792023 MsaT004284.1:CDS
TAGACACACACTTTCATCAT+TGG 0.402470 1:+67791819 None:intergenic
GTAGGAACTGGCTTGGGACT+TGG 0.405420 1:-67793937 MsaT004284.1:CDS
GAGGGGCTAACATGGAGTTT+TGG 0.406382 1:+67792450 None:intergenic
AACACTCTTGTGGTTAGTCT+TGG 0.410012 1:+67794158 None:intergenic
AGTCTTCGGAGATGAGTTCT+AGG 0.419954 1:-67792242 MsaT004284.1:CDS
ATCTTACAAAGCTTAGAAAA+TGG 0.421080 1:+67792072 None:intergenic
CCACCATACTCAATGAATAC+TGG 0.421080 1:-67797155 None:intergenic
AACAATCTACTACCATGAAA+TGG 0.422179 1:+67791931 None:intergenic
ATTAGGCTTCTTCCTGAGTT+CGG 0.422475 1:-67791901 MsaT004284.1:CDS
ACATTTCAATCAAACCAATA+AGG 0.422617 1:+67794776 None:intergenic
CAGTTGTTCATAGAGAGCTT+AGG 0.425096 1:-67792363 MsaT004284.1:CDS
TTTGTTGATGATGATTTCAT+TGG 0.427329 1:+67796763 None:intergenic
TCATATTTGGCTACCAAAGA+TGG 0.429385 1:-67795967 MsaT004284.1:CDS
ATGGCTACTTTGAGATCTCT+TGG 0.429609 1:-67791625 MsaT004284.1:CDS
TTATATCTTAATGGATTGTC+AGG 0.430305 1:-67791509 MsaT004284.1:intron
CCATAAGACATGGATCTTGT+TGG 0.432881 1:+67794064 None:intergenic
AGTTGTTCATAGAGAGCTTA+GGG 0.434205 1:-67792362 MsaT004284.1:CDS
TCCTAGTAAAGTTGTTTGGT+TGG 0.434623 1:-67792019 MsaT004284.1:CDS
TTGGTCTCCAATTCTGAACT+TGG 0.436377 1:+67794706 None:intergenic
CATAAGACATGGATCTTGTT+GGG 0.439105 1:+67794065 None:intergenic
GACAAGCTGACCACTGAGAC+TGG 0.440049 1:-67791274 MsaT004284.1:CDS
AAGAGTTCCTTTCAAAGCTA+TGG 0.440702 1:-67792190 MsaT004284.1:CDS
CCTTTCAAAGCTATGGATCA+TGG 0.441919 1:-67792183 MsaT004284.1:CDS
TCAAAGTTCACCGGAGTCTT+CGG 0.442260 1:-67792256 MsaT004284.1:CDS
AATCCAAGTCATAAAGAAAA+GGG 0.444621 1:-67795009 MsaT004284.1:CDS
CACTGTCTCATTCTCACATA+AGG 0.445103 1:+67792575 None:intergenic
GAAAGGTGTAGAAGTTGTAT+TGG 0.447417 1:-67794340 MsaT004284.1:CDS
TCTGTTGGCCAAGTAGGAAC+TGG 0.448175 1:-67793949 MsaT004284.1:CDS
TATCATCTTGATCAATTGCT+TGG 0.448900 1:+67791968 None:intergenic
GGAGACCAACTTAAAACAAA+TGG 0.449087 1:-67794692 MsaT004284.1:CDS
TAATCCAAGTCATAAAGAAA+AGG 0.450153 1:-67795010 MsaT004284.1:CDS
ATTATCATTGATGCAAATGC+AGG 0.450739 1:-67792096 MsaT004284.1:CDS
GACACTCTTGATTGTAATGA+TGG 0.452822 1:-67794983 MsaT004284.1:CDS
TATGTTAATCTGGGAAGAAA+AGG 0.456024 1:-67791760 MsaT004284.1:CDS
ATGGTAATGGAGATCTAATA+AGG 0.458097 1:-67795870 MsaT004284.1:CDS
GTTGTGTTATTGTAGGCATA+TGG 0.458179 1:-67796925 MsaT004284.1:intron
GCCTCAAACCCATCCTTCAC+TGG 0.462390 1:+67791370 None:intergenic
GTCCCAAGCCAGTTCCTACT+TGG 0.464624 1:+67793941 None:intergenic
TAGGAACTGGCTTGGGACTT+GGG 0.466445 1:-67793936 MsaT004284.1:CDS
GGCCAAGTAGGAACTGGCTT+GGG 0.467713 1:-67793943 MsaT004284.1:CDS
GAGAATTCTGTTGGCCAAGT+AGG 0.471261 1:-67793955 MsaT004284.1:CDS
AGAATAGGAGGGGTCTCTCT+TGG 0.472858 1:-67796051 MsaT004284.1:CDS
GAAAATAGTGACATAATGTA+AGG 0.473865 1:-67791187 None:intergenic
TGATATAATGGGAATAGAAT+CGG 0.482331 1:-67795758 MsaT004284.1:intron
TTGATCACTGAGCCATTACA+AGG 0.483090 1:+67794315 None:intergenic
GCCTCTGAGAGGGGCTAACA+TGG 0.484367 1:+67792442 None:intergenic
ACTTGGGATTGTGCAGTCAT+TGG 0.486079 1:-67793920 MsaT004284.1:intron
TCCATTAAGATATAATCATA+AGG 0.487002 1:+67791517 None:intergenic
TCAGCTAGAATCAACAGAAT+AGG 0.488864 1:-67796066 MsaT004284.1:CDS
AGCAATCCTAAACCATTAAG+TGG 0.490150 1:-67791697 MsaT004284.1:CDS
GCCAGTGAAGGATGGGTTTG+AGG 0.491157 1:-67791371 MsaT004284.1:CDS
TCTTGATAGGTTTGGTAATG+TGG 0.491525 1:+67791242 None:intergenic
TAATCAACACTACTCTCAAA+AGG 0.491934 1:+67791991 None:intergenic
TGATTTACCTAGCAAATCTT+GGG 0.494105 1:+67794660 None:intergenic
ATTCACGTGTGAAAGGTGAT+AGG 0.495625 1:-67794290 MsaT004284.1:CDS
TTCAATGCTTGAAATAATAA+CGG 0.495873 1:+67796396 None:intergenic
GTTGTATTGGACCCTTGTAA+TGG 0.497519 1:-67794327 MsaT004284.1:CDS
GGTATTGTGGACAAGGAAGT+TGG 0.498320 1:-67792636 MsaT004284.1:CDS
CAAGTTAAAGAGAATTCTGT+TGG 0.500351 1:-67793964 MsaT004284.1:CDS
CAAGATAAGCACGAAGATCA+TGG 0.500470 1:+67794803 None:intergenic
CAGGAAGAAGCCTAATGACT+TGG 0.500658 1:+67791908 None:intergenic
TTGACACAAATCTCATACAT+AGG 0.502362 1:-67796360 MsaT004284.1:CDS
AGGAGGATTAGTAAAATCCT+TGG 0.504201 1:+67791537 None:intergenic
TTCTAGGTACGTTTGATGCA+TGG 0.504400 1:-67792666 MsaT004284.1:intron
TATGCTGAGAAAGATTTGTA+TGG 0.505750 1:-67791644 MsaT004284.1:CDS
ATTCCAGTGTCATGGTTCAC+AGG 0.507966 1:+67796228 None:intergenic
CATCCAATGGCAATGAAGAA+AGG 0.508058 1:-67794357 MsaT004284.1:CDS
CAGTGTCATGGTTCACAGGT+TGG 0.508152 1:+67796232 None:intergenic
GACAGATGCCAGTGAAGGAT+GGG 0.510140 1:-67791378 MsaT004284.1:intron
CCATGATCCATAGCTTTGAA+AGG 0.512640 1:+67792183 None:intergenic
AATGAATACTGGTTTATCAA+TGG 0.512649 1:-67797144 MsaT004284.1:CDS
CAAAAGTCCTATGTTAATCT+GGG 0.514695 1:-67791769 MsaT004284.1:CDS
GACAAGGAAGTTGGCAAAAG+AGG 0.518131 1:-67792627 MsaT004284.1:CDS
ATGATAATCAAAACAAAACT+TGG 0.518308 1:+67792111 None:intergenic
TGGAGATCTAATAAGGAATG+AGG 0.518743 1:-67795863 MsaT004284.1:CDS
CTATCAATATTGATGCAAGT+TGG 0.529019 1:-67796173 MsaT004284.1:CDS
GCAATCAGAAATTGAGTACT+CGG 0.530081 1:-67796800 MsaT004284.1:CDS
CGTTTGATGCATGGAGACAT+TGG 0.531016 1:-67792657 MsaT004284.1:CDS
GGACAGATGCCAGTGAAGGA+TGG 0.536750 1:-67791379 MsaT004284.1:intron
GAGCTTAGGGATTAAAGTCA+AGG 0.538142 1:-67792349 MsaT004284.1:CDS
GAAGGCTCAGGCCTCTGAGA+GGG 0.538284 1:+67792432 None:intergenic
AATTGTGCCCAAGATTTGCT+AGG 0.541010 1:-67794667 MsaT004284.1:intron
ATCTCATACATAGGCATGGA+AGG 0.541087 1:-67796351 MsaT004284.1:CDS
GACAATGCGAGTGATATAAT+CGG 0.541959 1:+67796008 None:intergenic
CTTTCTCTCAGCTTGTTCTG+TGG 0.542457 1:+67794861 None:intergenic
TATGAGTATGGATCAACCTC+AGG 0.543399 1:-67792549 MsaT004284.1:CDS
CATTCTTTACATTACTGATG+AGG 0.545789 1:-67792403 MsaT004284.1:CDS
CTCGCATTGTCGATCGTCAA+GGG 0.547004 1:-67795996 MsaT004284.1:CDS
TATTTGAAGTGGATGATACA+GGG 0.550570 1:-67794035 MsaT004284.1:CDS
CAAGGTGAAATAGAAGAAAG+TGG 0.553365 1:-67791727 MsaT004284.1:CDS
GCTAGAATCAACAGAATAGG+AGG 0.555811 1:-67796063 MsaT004284.1:CDS
GACACAAGCAAGATTGAGGC+AGG 0.556358 1:-67794447 MsaT004284.1:CDS
TGTACTCTAACTCATCTAGT+GGG 0.561197 1:-67796284 MsaT004284.1:CDS
ACTATTGACCAATGTGAGAA+TGG 0.561890 1:-67791595 MsaT004284.1:CDS
AGAGAGATGGAGAAACACTA+TGG 0.561980 1:-67791334 MsaT004284.1:CDS
ATCAAACATTCACGTGTGAA+AGG 0.562833 1:-67794297 MsaT004284.1:CDS
AGCAAGATCATGAAAGATGA+AGG 0.564158 1:+67797110 None:intergenic
CTGTTAAATTTACTGATGAA+GGG 0.567152 1:-67794227 MsaT004284.1:CDS
GACACCATGTTGGTATCTCA+TGG 0.568120 1:-67796878 MsaT004284.1:CDS
TGGCCAAGTAGGAACTGGCT+TGG 0.570068 1:-67793944 MsaT004284.1:CDS
TCTTGCAAGGAGCAAGCTGT+TGG 0.570849 1:-67795832 MsaT004284.1:CDS
AGGATGGGTTTGAGGCAACA+AGG 0.571342 1:-67791363 MsaT004284.1:CDS
ACTCGCATTGTCGATCGTCA+AGG 0.572216 1:-67795997 MsaT004284.1:CDS
AGTTGATTTGTATCATCCAA+TGG 0.573037 1:-67794370 MsaT004284.1:CDS
ATGTAAAGAATGACATGTGA+AGG 0.573120 1:+67792414 None:intergenic
CACACACTTTCATCATTGGA+AGG 0.574607 1:+67791823 None:intergenic
GGAGATCTAATAAGGAATGA+GGG 0.576644 1:-67795862 MsaT004284.1:CDS
GTGTTATGAAACATTTGTCT+TGG 0.576661 1:-67794140 MsaT004284.1:CDS
GGCTGTGAGAACTGTTCAAG+AGG 0.577188 1:-67791566 MsaT004284.1:CDS
ATCCAATTACCACCGAACTC+AGG 0.583478 1:+67791889 None:intergenic
GCTGACCACTGAGACTGGAA+TGG 0.584171 1:-67791269 MsaT004284.1:CDS
ACTTGACACAAGCAAGATTG+AGG 0.587753 1:-67794451 MsaT004284.1:CDS
TTGCCTCATTGACACCATGT+TGG 0.593071 1:-67796888 MsaT004284.1:CDS
AAGTGTGGAACAATGTTGGA+AGG 0.593740 1:-67791797 MsaT004284.1:CDS
GCATGGAGACATTGGTATTG+TGG 0.595262 1:-67792649 MsaT004284.1:CDS
AGAAAAGGTTCTGTTCATCA+AGG 0.596627 1:-67791745 MsaT004284.1:CDS
CCTCTTCACCATTCTCACAT+TGG 0.597986 1:+67791587 None:intergenic
ATACAAATCTTTCTCAGCAT+AGG 0.598094 1:+67791646 None:intergenic
AGGCTTCTTCCTGAGTTCGG+TGG 0.599749 1:-67791898 MsaT004284.1:CDS
GGCACATAACAGTTAGAGCA+TGG 0.600016 1:-67794206 MsaT004284.1:CDS
TATCAGTGATCAAAGTTCAC+CGG 0.601334 1:-67792265 MsaT004284.1:CDS
TGATTTATCCTCTCTCCTTG+AGG 0.602251 1:-67794397 MsaT004284.1:CDS
TGAGGGAGCTGTATCTTGCA+AGG 0.603521 1:-67795845 MsaT004284.1:CDS
AAGGCTCAGGCCTCTGAGAG+GGG 0.603713 1:+67792433 None:intergenic
AGAATGACATGTGAAGGCTC+AGG 0.609075 1:+67792420 None:intergenic
AACAAGGGAGATAAGAGAGA+TGG 0.610075 1:-67791347 MsaT004284.1:CDS
GAAGTGGATGATACAGGGAA+AGG 0.611039 1:-67794030 MsaT004284.1:CDS
GTCTAAGTGTGGAACAATGT+TGG 0.612167 1:-67791801 MsaT004284.1:CDS
ACAAATCTCATACATAGGCA+TGG 0.612469 1:-67796355 MsaT004284.1:CDS
ATGTACTCTAACTCATCTAG+TGG 0.614715 1:-67796285 MsaT004284.1:CDS
GAACTCATCTCCGAAGACTC+CGG 0.615301 1:+67792246 None:intergenic
CAACCTGTGAACCATGACAC+TGG 0.615905 1:-67796231 MsaT004284.1:CDS
AATGTTTCATAACACTCTTG+TGG 0.617188 1:+67794148 None:intergenic
AACTACATCCTCAAGGAGAG+AGG 0.617424 1:+67794389 None:intergenic
AGACATTGGTATTGTGGACA+AGG 0.619613 1:-67792643 MsaT004284.1:CDS
TGTTCAAGAGGGTTTAACCA+AGG 0.619844 1:-67791554 MsaT004284.1:CDS
GGATGGGTTTGAGGCAACAA+GGG 0.621462 1:-67791362 MsaT004284.1:CDS
GTACTCTAACTCATCTAGTG+GGG 0.623166 1:-67796283 MsaT004284.1:CDS
AACATGGTGTCAATGAGGCA+AGG 0.632743 1:+67796890 None:intergenic
AGTGTGGAACAATGTTGGAA+GGG 0.632865 1:-67791796 MsaT004284.1:CDS
CTAGAATCAACAGAATAGGA+GGG 0.634091 1:-67796062 MsaT004284.1:CDS
ACAAATCAACTACATCCTCA+AGG 0.638204 1:+67794382 None:intergenic
AATACAAAATCCATAAGACA+TGG 0.641986 1:+67794054 None:intergenic
GCTGTGAGAACTGTTCAAGA+GGG 0.647146 1:-67791565 MsaT004284.1:CDS
TACTCTAACTCATCTAGTGG+GGG 0.650632 1:-67796282 MsaT004284.1:CDS
CTTGGTGTGAACATCAGAGA+AGG 0.651051 1:+67796696 None:intergenic
CCAATGTGAGAATGGTGAAG+AGG 0.657628 1:-67791587 MsaT004284.1:CDS
TGAAGGCTCAGGCCTCTGAG+AGG 0.658399 1:+67792431 None:intergenic
TGAAAGTGTGTGTCTAAGTG+TGG 0.660501 1:-67791812 MsaT004284.1:CDS
TGTGAACCATGACACTGGAA+TGG 0.664217 1:-67796226 MsaT004284.1:CDS
ATACCAACATGGTGTCAATG+AGG 0.665423 1:+67796885 None:intergenic
ATTAAGATATAATCATAAGG+AGG 0.668188 1:+67791520 None:intergenic
AATTGGACAGATGCCAGTGA+AGG 0.672213 1:-67791383 MsaT004284.1:intron
TATGAGATTTGTGTCAAGTG+AGG 0.675612 1:+67796366 None:intergenic
GAAATCCATTCCAGTCTCAG+TGG 0.681772 1:+67791264 None:intergenic
TAGAATCAACAGAATAGGAG+GGG 0.688378 1:-67796061 MsaT004284.1:CDS
TCCATGTTAGCCCCTCTCAG+AGG 0.694979 1:-67792443 MsaT004284.1:CDS
TGATCACTGAGCCATTACAA+GGG 0.704208 1:+67794316 None:intergenic
CTTCTCTGATGTTCACACCA+AGG 0.706528 1:-67796695 MsaT004284.1:intron
CTATTCCCATTATATCAATG+GGG 0.724239 1:+67795764 None:intergenic
GTGACCATGAGATACCAACA+TGG 0.814649 1:+67796874 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 67791189 67797165 67791189 ID=MsaG004284
Chr1 mRNA 67791189 67797165 67791189 ID=MsaT004284.1;Parent=MsaG004284
Chr1 exon 67791189 67791395 67791189 ID=MsaT004284.1.exon7;Parent=MsaT004284.1
Chr1 CDS 67791189 67791395 67791189 ID=cds.MsaT004284.1;Parent=MsaT004284.1
Chr1 exon 67791510 67792682 67791510 ID=MsaT004284.1.exon6;Parent=MsaT004284.1
Chr1 CDS 67791510 67792682 67791510 ID=cds.MsaT004284.1;Parent=MsaT004284.1
Chr1 exon 67793921 67794489 67793921 ID=MsaT004284.1.exon5;Parent=MsaT004284.1
Chr1 CDS 67793921 67794489 67793921 ID=cds.MsaT004284.1;Parent=MsaT004284.1
Chr1 exon 67794668 67795073 67794668 ID=MsaT004284.1.exon4;Parent=MsaT004284.1
Chr1 CDS 67794668 67795073 67794668 ID=cds.MsaT004284.1;Parent=MsaT004284.1
Chr1 exon 67795759 67796424 67795759 ID=MsaT004284.1.exon3;Parent=MsaT004284.1
Chr1 CDS 67795759 67796424 67795759 ID=cds.MsaT004284.1;Parent=MsaT004284.1
Chr1 exon 67796696 67796932 67796696 ID=MsaT004284.1.exon2;Parent=MsaT004284.1
Chr1 CDS 67796696 67796932 67796696 ID=cds.MsaT004284.1;Parent=MsaT004284.1
Chr1 exon 67797109 67797165 67797109 ID=MsaT004284.1.exon1;Parent=MsaT004284.1
Chr1 CDS 67797109 67797165 67797109 ID=cds.MsaT004284.1;Parent=MsaT004284.1
Gene Sequence

>MsaT004284.1

ATGAATACTGGTTTATCAATGGCATTACTTCGACCTTCATCTTTCATGATCTTGCTGGCATATGGTGCACTAATAGTCCTTGCCTCATTGACACCATGTTGGTATCTCATGGTCACACATATTGAGAAAAGTGTGAATTTGCACTCAGCAAATATTGTGTCACAATTGCAATCAGAAATTGAGTACTCGGCTGAACTATTGCAACCAATGAAATCATCATCAACAAATTTATCTACATTATTGAGCTCAACCCTTAATTCTACTAATGTCACCTTCTCTGATGTTCACACCAAGGTGGCTCCGTTATTATTTCAAGCATTGAAGACAATTCCTCACTTGACACAAATCTCATACATAGGCATGGAAGGTCTTTTTTTCACACACTACAATGATGGTGATCAAGTTCTAGCAATGTACTCTAACTCATCTAGTGGGGGTGCTTCAAACAAAACCATATACTATATCCAACCTGTGAACCATGACACTGGAATGGTTTTTGGGGAAGCCATAATATCTAACAACACTATCAATATTGATGCAAGTTGGATAAATGGAACAAATAATATTTCACATGAATTTGCATCATTGGGAACAAAGTTGAATAATGTTAGTGATCTTTTGTTCATGAATTCAGCTAGAATCAACAGAATAGGAGGGGTCTCTCTTGGATTTTCGACGAAAGCAATAACCGATTATATCACTCGCATTGTCGATCGTCAAGGGACAAAATCATATTTGGCTACCAAAGATGGAAAAGTTATTGTAAAAGGAATTCAAAATATCCGTTTGATGATTTTTAATGATAGTGTTTCAATTCAAGCTGTTAATGGTAATGGAGATCTAATAAGGAATGAGGGAGCTGTATCTTGCAAGGAGCAAGCTGTTGGTTCTAGTTTGAATATTCATGATACTCAATATTTGATTCATTGCTACCCCATTGATATAATGGGAATAGAATCGGTGCATGTGTTAGCTGTCCCACAAAATGGATCTCTCATCTTTAATCCAAGTCATAAAGAAAAGGGATTGACACTCTTGATTGTAATGATGGTGATGATATTTATTGCTATCTTGAGTTTTCTATTCTTGAATGTTGGAGTGACAAAGAGAGAAATGCATTTGTGTGCATCACTCATACAACAAAAAGAAGCCACAGAACAAGCTGAGAGAAAGAATACCAATAAGAGTCTTGCTTTTGCTACTGCTAGCCATGATCTTCGTGCTTATCTTGCAGGCCTTATTGGTTTGATTGAAATGTCCTCTAAATTAGTTGTTACTAATTCAAAATTGGACACCAATTTGAAACCAAGTTCAGAATTGGAGACCAACTTAAAACAAATGGATAATTGTGCCCAAGATTTGCTAGGACTGTTGAATTCTATACTTGACACAAGCAAGATTGAGGCAGGAAAAATGCAACTAGAGGAAGAGGAATTTGATTTATCCTCTCTCCTTGAGGATGTAGTTGATTTGTATCATCCAATGGCAATGAAGAAAGGTGTAGAAGTTGTATTGGACCCTTGTAATGGCTCAGTGATCAAACATTCACGTGTGAAAGGTGATAGGAGAAAATTGAAGCAAGTTTTGTGTAACTTGCTAAGCAATGCTGTTAAATTTACTGATGAAGGGCACATAACAGTTAGAGCATGGACTCAGAAAGCCAAATTGCAAAAATCCAAGACTAACCACAAGAGTGTTATGAAACATTTGTCTTGGTTGTTTAGTAAGAAAGACAAAGAATGTGAAGACATAGAAGCTGTGAATTTAGCCCAACAAGATCCATGTCTTATGGATTTTGTATTTGAAGTGGATGATACAGGGAAAGGTATTCCTAAAGAGAATCACAAGTCAGTGTTTGAGAATTATGTTCAAGTTAAAGAGAATTCTGTTGGCCAAGTAGGAACTGGCTTGGGACTTGGGATTGTGCAGTCATTGGTACGTTTGATGCATGGAGACATTGGTATTGTGGACAAGGAAGTTGGCAAAAGAGGAACATGCTTCAAGTTCAATGTGCTTCTCACCTTATGTGAGAATGAGACAGTGAATTATGAGTATGGATCAACCTCAGGTGACAAAAACCAAACCCTAGAGAAAAAATTAACAATTCGTACTGCTAGTTCTGGTTCAAGCATTTGTTCTTTGAGTCCAAAACTCCATGTTAGCCCCTCTCAGAGGCCTGAGCCTTCACATGTCATTCTTTACATTACTGATGAGGAAAGAAGAAGAACTTCGCAGTTGTTCATAGAGAGCTTAGGGATTAAAGTCAAGGTTGTGAAGAGTAGAAATCATCTAATTCATACTCTCAAGAAGATCAAAAAAGAAAAGGGTGATATCAGTGATCAAAGTTCACCGGAGTCTTCGGAGATGAGTTCTAGGTGTACATCTTATAACTCTTCTTGTAGTAGAAGAGTTCCTTTCAAAGCTATGGATCATGGAAATGAATATCTATCTTCAATGTTCAAGAAGAAAAACAATGGAGCTGCACCAAGTTTTGTTTTGATTATCATTGATGCAAATGCAGGACCATTTTCTAAGCTTTGTAAGATTGTGTCAAACTTCAAGAAAGATCTTCTCAATCCTAGTAAAGTTGTTTGGTTGGAAAAGCCTTTTGAGAGTAGTGTTGATTACCAAGCAATTGATCAAGATGATATTGTGATATCAAAACCATTTCATGGTAGTAGATTGTTCCAAGTCATTAGGCTTCTTCCTGAGTTCGGTGGTAATTGGATAAGCAATTCTAGTAAACCAAGAAATGAACCTAGATCACAAGTACCTTCCAATGATGAAAGTGTGTGTCTAAGTGTGGAACAATGTTGGAAGGGAACACAAAAGTCCTATGTTAATCTGGGAAGAAAAGGTTCTGTTCATCAAGGTGAAATAGAAGAAAGTGGAGAATCAAGCAATCCTAAACCATTAAGTGGAAAGAAGTTTTTGGTTGTTGATGATAGTCCTATGCTGAGAAAGATTTGTATGGCTACTTTGAGATCTCTTGGTGTGACTACTATTGACCAATGTGAGAATGGTGAAGAGGCTGTGAGAACTGTTCAAGAGGGTTTAACCAAGGATTTTACTAATCCTCCTTATGATTATATCTTAATGGATTGTCAGATGCCAGTGAAGGATGGGTTTGAGGCAACAAGGGAGATAAGAGAGATGGAGAAACACTATGGTCTTCACATTTCTATCATTGCATTATCTGCTGAAATTGACAAGCTGACCACTGAGACTGGAATGGATTTCCACATTACCAAACCTATCAAGAAAGAGCATTTACTCAAAGCTATTGAGTACATAGAAAATAGTGACATAATGTAA
Protein sequence

>MsaT004284.1

MNTGLSMALLRPSSFMILLAYGALIVLASLTPCWYLMVTHIEKSVNLHSANIVSQLQSEIEYSAELLQPMKSSSTNLSTLLSSTLNSTNVTFSDVHTKVAPLLFQALKTIPHLTQISYIGMEGLFFTHYNDGDQVLAMYSNSSSGGASNKTIYYIQPVNHDTGMVFGEAIISNNTINIDASWINGTNNISHEFASLGTKLNNVSDLLFMNSARINRIGGVSLGFSTKAITDYITRIVDRQGTKSYLATKDGKVIVKGIQNIRLMIFNDSVSIQAVNGNGDLIRNEGAVSCKEQAVGSSLNIHDTQYLIHCYPIDIMGIESVHVLAVPQNGSLIFNPSHKEKGLTLLIVMMVMIFIAILSFLFLNVGVTKREMHLCASLIQQKEATEQAERKNTNKSLAFATASHDLRAYLAGLIGLIEMSSKLVVTNSKLDTNLKPSSELETNLKQMDNCAQDLLGLLNSILDTSKIEAGKMQLEEEEFDLSSLLEDVVDLYHPMAMKKGVEVVLDPCNGSVIKHSRVKGDRRKLKQVLCNLLSNAVKFTDEGHITVRAWTQKAKLQKSKTNHKSVMKHLSWLFSKKDKECEDIEAVNLAQQDPCLMDFVFEVDDTGKGIPKENHKSVFENYVQVKENSVGQVGTGLGLGIVQSLVRLMHGDIGIVDKEVGKRGTCFKFNVLLTLCENETVNYEYGSTSGDKNQTLEKKLTIRTASSGSSICSLSPKLHVSPSQRPEPSHVILYITDEERRRTSQLFIESLGIKVKVVKSRNHLIHTLKKIKKEKGDISDQSSPESSEMSSRCTSYNSSCSRRVPFKAMDHGNEYLSSMFKKKNNGAAPSFVLIIIDANAGPFSKLCKIVSNFKKDLLNPSKVVWLEKPFESSVDYQAIDQDDIVISKPFHGSRLFQVIRLLPEFGGNWISNSSKPRNEPRSQVPSNDESVCLSVEQCWKGTQKSYVNLGRKGSVHQGEIEESGESSNPKPLSGKKFLVVDDSPMLRKICMATLRSLGVTTIDQCENGEEAVRTVQEGLTKDFTNPPYDYILMDCQMPVKDGFEATREIREMEKHYGLHISIIALSAEIDKLTTETGMDFHITKPIKKEHLLKAIEYIENSDIM*