AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004289

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004289 MtrunA17_Chr1g0194861 90.311 1352 103 7 1 1330 1 1346 0.0 2445
MsaG004289 MtrunA17_Chr6g0456701 33.684 95 61 2 92 185 4 97 4.24e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004289 AT4G02110.2 70.870 230 66 1 900 1129 967 1195 3.69e-114 387
MsaG004289 AT4G02110.2 64.486 107 32 2 110 215 1 102 3.35e-34 143
MsaG004289 AT4G02110.1 70.870 230 66 1 900 1129 1096 1324 9.86e-113 386
MsaG004289 AT4G02110.1 63.507 211 71 2 6 215 26 231 2.77e-80 290
MsaG004289 AT1G67180.6 34.906 106 58 5 90 186 2 105 7.69e-11 66.2

Find 302 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAGAGTCTTTACCCTTAT+TGG 0.075467 1:+67879593 None:intergenic
GTTTGGGTGCTACTGCATTT+TGG 0.133192 1:+67876330 None:intergenic
GATTTCCTGGTCTTGATAAT+TGG 0.143495 1:-67878573 MsaT004289.1:CDS
CTTTGCAGCTGTGGTCTTCT+TGG 0.147073 1:+67877979 None:intergenic
ACTTGTGGACCAGTAGAATT+TGG 0.162831 1:+67879627 None:intergenic
TCCATCTATATCAGAATTTA+AGG 0.164801 1:-67877669 MsaT004289.1:CDS
AATTTCTCTGTCCTCCTTAT+AGG 0.170617 1:+67877494 None:intergenic
AAAATAAAACATGTGGTTTC+AGG 0.179171 1:+67877635 None:intergenic
AAATCGGAAAAGGCAGTATT+TGG 0.201132 1:-67877947 MsaT004289.1:CDS
TATACTCGCTTTCTTGATAC+TGG 0.214753 1:-67876765 MsaT004289.1:CDS
ATTTGTTAACATCAAGGATT+TGG 0.217175 1:+67878329 None:intergenic
AAAAGTCAATAAAAGTAAAA+AGG 0.225243 1:-67875822 MsaT004289.1:CDS
ACAGGTCATGGTGCCTTCTA+TGG 0.228805 1:-67877188 MsaT004289.1:CDS
AAAACCCAATAAAAGGAAAA+AGG 0.231177 1:-67876292 MsaT004289.1:CDS
TCTTGTGTAACTTAACTTCT+TGG 0.243171 1:+67879317 None:intergenic
GCCTCATCTCTTGTCTCATT+TGG 0.250185 1:+67877914 None:intergenic
TTCAACAGCCGCCACTTTCT+TGG 0.251038 1:+67877218 None:intergenic
AGTCCCCTAGGTTCAAAATT+TGG 0.268181 1:-67879708 MsaT004289.1:CDS
TCATTCTTTGAATCCTTTCC+TGG 0.269576 1:+67878598 None:intergenic
TAAAGATTTGGATGGAATTC+CGG 0.270747 1:-67881814 MsaT004289.1:CDS
TCCAAAGGCACCTGGCTTCC+AGG 0.273647 1:-67879409 MsaT004289.1:CDS
CAGACAGTGACATTTCTTTC+AGG 0.274545 1:+67877879 None:intergenic
TCCTTAGACTTGATATTTGC+TGG 0.278398 1:+67877704 None:intergenic
ATTCGATTCAAGCTTGTTAA+TGG 0.292549 1:-67883867 MsaT004289.1:CDS
TTCTGCCTTACTTTGATGTT+TGG 0.298597 1:+67876075 None:intergenic
TGCTATTTCTTTGCTTCTGC+TGG 0.302908 1:+67878384 None:intergenic
GTACAGACCGCTTAAAGATT+TGG 0.314245 1:-67881826 MsaT004289.1:CDS
CCTTGATGTTAACAAATTGA+TGG 0.316106 1:-67878323 MsaT004289.1:CDS
TATTCTAGGAAGAACCAAAA+AGG 0.316958 1:-67879234 MsaT004289.1:CDS
TTTGTTAACATCAAGGATTT+GGG 0.318611 1:+67878330 None:intergenic
GATTCCAAGCTGGGGCAATC+AGG 0.318812 1:-67879750 MsaT004289.1:CDS
CCAGGATCGGGATGACATTA+TGG 0.318910 1:-67881751 MsaT004289.1:intron
AAAGATTTGGATGGAATTCC+GGG 0.320376 1:-67881813 MsaT004289.1:CDS
TTCCAGGATATTGACATTAC+TGG 0.328872 1:-67879393 MsaT004289.1:CDS
TCTGTCCTCCTTATAGGATC+TGG 0.341349 1:+67877500 None:intergenic
TAATCTGCGTGATGACAATT+TGG 0.341472 1:-67879343 MsaT004289.1:CDS
GTATCAAGAAAGCGAGTATA+AGG 0.343349 1:+67876768 None:intergenic
AAACCATGCTCTTCATCATT+TGG 0.346087 1:+67877776 None:intergenic
TCTTTACCCTTATTGGCATT+TGG 0.346613 1:+67879600 None:intergenic
GAGGATTGCAGCAATCGATA+TGG 0.347128 1:+67875912 None:intergenic
GCCAGCAAATATCAAGTCTA+AGG 0.349651 1:-67877705 MsaT004289.1:CDS
TCCTGGAAGCCAGGTGCCTT+TGG 0.349876 1:+67879408 None:intergenic
GCCTTAAATTCTGATATAGA+TGG 0.350253 1:+67877668 None:intergenic
TCATAGCTCCAGATCCTATA+AGG 0.358776 1:-67877508 MsaT004289.1:CDS
GCATGACATCTCCTCTTTCC+AGG 0.359580 1:+67876457 None:intergenic
TCTGCATGTGTTGAAGGTTC+TGG 0.362025 1:-67879111 MsaT004289.1:CDS
GATTCTGCATTTCCTGAGAT+AGG 0.362624 1:+67878784 None:intergenic
CAGCTGTTCCTGAGGAGGAT+TGG 0.366522 1:-67876359 MsaT004289.1:CDS
ATTTCCTGGTCTTGATAATT+GGG 0.369870 1:-67878572 MsaT004289.1:CDS
CGAGGACGGTGCCATCAATA+TGG 0.373926 1:-67877246 MsaT004289.1:CDS
TCTTGGAGCCTCCATATTGA+TGG 0.375100 1:+67877235 None:intergenic
TGCAAAGAAGTTAGCGAAAT+CGG 0.379072 1:-67877963 MsaT004289.1:CDS
TTGCGCGGTGACTTTCTAAT+CGG 0.381507 1:+67878523 None:intergenic
GTTGAGTACGTCTGCAAGCC+GGG 0.382287 1:-67876642 MsaT004289.1:CDS
GATTTGGTTGTCTGCTTGAC+TGG 0.387720 1:-67881783 MsaT004289.1:CDS
CTAATATGCTACAAATTTGA+AGG 0.393357 1:-67881568 MsaT004289.1:intron
TGTTAATTTCTTCAGCTTGA+AGG 0.393387 1:-67880525 MsaT004289.1:intron
TCAGCTGCCAAGAGAACTTT+GGG 0.395676 1:-67878496 MsaT004289.1:CDS
AATTCCGGGTGCAAAAGATT+TGG 0.397200 1:-67881799 MsaT004289.1:CDS
TCAAAGAATGAAGGATTTCC+TGG 0.397634 1:-67878586 MsaT004289.1:CDS
AAATCGACACGTGGCATGCC+AGG 0.401546 1:+67876720 None:intergenic
CTGATTTGTGGTGATGAAAC+TGG 0.403537 1:-67875959 MsaT004289.1:CDS
AATCGGAAAAGGCAGTATTT+GGG 0.404273 1:-67877946 MsaT004289.1:CDS
ATCACCGTTGGCTGGAAGAT+TGG 0.404581 1:-67880670 MsaT004289.1:intron
TTCTCATCAGTGGTCATATC+AGG 0.407810 1:-67877540 MsaT004289.1:CDS
ATTTCTTCAGCTTGAAGGAT+TGG 0.411153 1:-67880520 MsaT004289.1:intron
ATTTGCAAAACTTCAAAGTA+AGG 0.412007 1:-67876565 MsaT004289.1:CDS
CTTAAGCGTGCGGTCAAAGC+TGG 0.412816 1:-67876819 MsaT004289.1:CDS
TTTCTTCAGCTTGAAGGATT+GGG 0.414914 1:-67880519 MsaT004289.1:intron
GAGATTTACCATTTGTTAGT+GGG 0.417900 1:+67878953 None:intergenic
CTTGTGGACCAGTAGAATTT+GGG 0.418686 1:+67879628 None:intergenic
GGGATGCGCATTGTTGTATA+TGG 0.419008 1:-67877167 MsaT004289.1:CDS
AATGGCTCCGGATGAAGAAT+TGG 0.419989 1:-67878197 MsaT004289.1:CDS
TGAGATTTACCATTTGTTAG+TGG 0.420188 1:+67878952 None:intergenic
CTTTATTGTGGCCAATCTCT+TGG 0.421271 1:+67880700 None:intergenic
AGCAAAGAAATAGCAACTTC+TGG 0.422190 1:-67878376 MsaT004289.1:CDS
TCTACTTCACATTTGTCATC+TGG 0.424117 1:+67878226 None:intergenic
GTAGAATGCAAAGAAACTTC+AGG 0.427783 1:+67879489 None:intergenic
AACAATGCGCATCCCATAGA+AGG 0.432187 1:+67877175 None:intergenic
TGGCATGCCAGGGCTAACTA+TGG 0.435261 1:+67876731 None:intergenic
ACTGGAAATACAAATTCATC+TGG 0.437293 1:-67879375 MsaT004289.1:CDS
TTCAAGCTTGTTAATGGCGG+TGG 0.438677 1:-67883861 MsaT004289.1:CDS
GTCAGCTGCCAAGAGAACTT+TGG 0.438819 1:-67878497 MsaT004289.1:CDS
TAGAAAACTGTGCACCCATC+AGG 0.438898 1:+67881620 None:intergenic
TTTCTTTCTGTTGACAAAGA+TGG 0.441291 1:-67878880 MsaT004289.1:CDS
TTTGAGGAGAGAAGAAATGT+TGG 0.441816 1:-67877755 MsaT004289.1:CDS
TCAGACAATGGTTGGCCTGA+TGG 0.442786 1:-67881635 MsaT004289.1:intron
CCCTTCCACCAAGGTAGAAA+AGG 0.443209 1:-67879151 MsaT004289.1:CDS
CCATCAATTTGTTAACATCA+AGG 0.444383 1:+67878323 None:intergenic
TGCCACTCATAAGGCTCCTC+TGG 0.446343 1:+67877284 None:intergenic
CAGCTATTCCCGAGGAGGAT+TGG 0.447918 1:-67875883 MsaT004289.1:CDS
TTCCCTCTTCAGACAATGGT+TGG 0.449094 1:-67881643 MsaT004289.1:intron
ATCAGCATTCTTAGACTCAA+TGG 0.450480 1:+67879434 None:intergenic
ACTGTACGGCACTCATGCTT+TGG 0.452603 1:-67876598 MsaT004289.1:CDS
ATACTCGCTTTCTTGATACT+GGG 0.453049 1:-67876764 MsaT004289.1:CDS
TTGAGGAGAGAAGAAATGTT+GGG 0.457765 1:-67877754 MsaT004289.1:CDS
GCATGCCACGTGTCGATTTG+TGG 0.463323 1:-67876716 MsaT004289.1:CDS
TCTTCTGCATACTCACTAAC+CGG 0.463631 1:+67878292 None:intergenic
CGCTAACTTCTTTGCAGCTG+TGG 0.465086 1:+67877970 None:intergenic
TCGGTGCTACGAGTTATTCT+AGG 0.468156 1:-67879248 MsaT004289.1:CDS
GCATTTGATAAGCCTATCTC+AGG 0.468199 1:-67878796 MsaT004289.1:CDS
CAGACAATGGTTGGCCTGAT+GGG 0.468396 1:-67881634 MsaT004289.1:CDS
ATCAAATCTGCATGTGTTGA+AGG 0.468581 1:-67879117 MsaT004289.1:CDS
ATTCCAAGCTGGGGCAATCA+GGG 0.469861 1:-67879749 MsaT004289.1:CDS
AAATGAGCTTGCCAAGAGAT+TGG 0.472892 1:-67880711 MsaT004289.1:CDS
TTTCCAGGGATCCACATACT+TGG 0.474283 1:+67876472 None:intergenic
TTGTGTTGTAGCGGACTACT+TGG 0.476668 1:-67876664 MsaT004289.1:CDS
CCATAATGTCATCCCGATCC+TGG 0.479361 1:+67881751 None:intergenic
CATGCCACGTGTCGATTTGT+GGG 0.481317 1:-67876715 MsaT004289.1:CDS
AGCTACATGAATCATATGTT+AGG 0.482525 1:-67879458 MsaT004289.1:CDS
CTGCATGTGTTGAAGGTTCT+GGG 0.482604 1:-67879110 MsaT004289.1:CDS
GGGCAAAACCAATCCTCCTC+GGG 0.485220 1:+67875875 None:intergenic
TTGATCTACTACCCTGGAAA+AGG 0.486609 1:+67879206 None:intergenic
GATGTTGGTGACTGCATTGT+GGG 0.486729 1:-67883653 MsaT004289.1:CDS
TTCCAGTAATGTCAATATCC+TGG 0.487192 1:+67879391 None:intergenic
TGGCTATCTACGCCAGGATC+GGG 0.488311 1:-67881763 MsaT004289.1:CDS
GAACCTGACAGCTTACCTTC+AGG 0.489934 1:+67879511 None:intergenic
ACTAGAGATGATGGCGGAAG+AGG 0.490099 1:-67879799 MsaT004289.1:CDS
GGGACAGACTCACTTTGCCT+TGG 0.490392 1:+67876254 None:intergenic
GCTAGTTAATCACCGTTGGC+TGG 0.491106 1:-67880678 MsaT004289.1:CDS
AGATGAACCAATTCTTCATC+CGG 0.491955 1:+67878190 None:intergenic
TTTCCCGCGATCCACATACT+TGG 0.492525 1:+67875996 None:intergenic
TCTAGAACCCAAAGTTCTCT+TGG 0.493789 1:+67878489 None:intergenic
AAATAACTTAAATACCTGCT+TGG 0.501492 1:-67878854 MsaT004289.1:CDS
GGTTGAGTACGTCTGCAAGC+CGG 0.501698 1:-67876643 MsaT004289.1:CDS
CAACCTGAAGGTAAGCTGTC+AGG 0.502452 1:-67879514 MsaT004289.1:CDS
TTTCTTAAGTGCCAGTAGCC+AGG 0.503878 1:-67877321 MsaT004289.1:CDS
AATGGCGGTGGAGTTGATGC+GGG 0.504030 1:-67883849 MsaT004289.1:CDS
GAAGGTTCTGGGAAAGAAAC+TGG 0.504396 1:-67879099 MsaT004289.1:CDS
CAAACCGAGGTAGCATCAAC+AGG 0.505536 1:+67883604 None:intergenic
TGGCCATCGCCTACAGAGAA+AGG 0.506713 1:-67877609 MsaT004289.1:CDS
GCACTCATGCTTTGGCAGAT+AGG 0.508633 1:-67876590 MsaT004289.1:CDS
GAGGCGGAAGATTCCAAGCT+GGG 0.509729 1:-67879759 MsaT004289.1:CDS
ATTTACCAAACATCAAAGTA+AGG 0.510575 1:-67876080 MsaT004289.1:CDS
GGGCAAAACCAATCCTCCTC+AGG 0.510620 1:+67876351 None:intergenic
AAAACTACTCAGCGAGATGA+AGG 0.510675 1:-67878418 MsaT004289.1:CDS
GATGAGCCACCTGTGTCTGA+TGG 0.510751 1:-67878904 MsaT004289.1:CDS
CAGGTCATGGTGCCTTCTAT+GGG 0.513908 1:-67877187 MsaT004289.1:CDS
TGATCAAGCGTTTGAAAGGA+AGG 0.513959 1:-67877574 MsaT004289.1:CDS
GTTCTTATCACAGGACGATC+CGG 0.515315 1:-67883704 MsaT004289.1:intron
ATAATTCATTGCCTCAAAAG+AGG 0.515513 1:-67879053 MsaT004289.1:CDS
GGAGATTTGATGCTGATTTG+TGG 0.515683 1:-67875971 MsaT004289.1:CDS
GAAGTTAGCGAAATCGGAAA+AGG 0.517733 1:-67877957 MsaT004289.1:CDS
GTTGATGCGGGGAAGAACAC+TGG 0.518035 1:-67883837 MsaT004289.1:CDS
TTAATCAAGTCTAAAGATAG+TGG 0.518083 1:-67878709 MsaT004289.1:CDS
GGCAGAAGAGATTATTGAGG+AGG 0.521403 1:-67876544 MsaT004289.1:CDS
AGGATTGCAGCAATCGATAT+GGG 0.522430 1:+67875913 None:intergenic
TCAACTTCTACAAGTGCTGA+TGG 0.523391 1:-67879294 MsaT004289.1:CDS
GATGATGGCGGAAGAGGCTA+AGG 0.523468 1:-67879793 MsaT004289.1:CDS
CTGGCTATCTACGCCAGGAT+CGG 0.524351 1:-67881764 MsaT004289.1:CDS
TTTGGCACAGCTGTGACTCA+TGG 0.524555 1:-67879690 MsaT004289.1:CDS
CGTGCGGTCAAAGCTGGAGA+TGG 0.526398 1:-67876813 MsaT004289.1:CDS
ATCAAATGCCAATGTGTTGG+AGG 0.526782 1:+67878810 None:intergenic
GTGATTAACTAGCTTTATTG+TGG 0.528417 1:+67880688 None:intergenic
TTAAGTGCCAGTAGCCAGGC+AGG 0.529869 1:-67877317 MsaT004289.1:CDS
GGCTAAGGATTCTGAGGATG+AGG 0.530245 1:-67879778 MsaT004289.1:CDS
AATGAGACAAGAGATGAGGC+AGG 0.530559 1:-67877911 MsaT004289.1:CDS
AAAGCCATCCCAAATTCTAC+TGG 0.533698 1:-67879636 MsaT004289.1:CDS
AGATTTACCATTTGTTAGTG+GGG 0.534103 1:+67878954 None:intergenic
CGTACAGTACGTGTCTCTCA+AGG 0.534451 1:+67876613 None:intergenic
GGAAAGGATTCAAAGAATGA+AGG 0.535665 1:-67878595 MsaT004289.1:CDS
GTATGTGGATCGCGGGAAAG+AGG 0.535687 1:-67875992 MsaT004289.1:CDS
AAAGACTCTTCGATTACTAC+AGG 0.536651 1:-67879582 MsaT004289.1:CDS
CTTGACTGGCTATCTACGCC+AGG 0.538587 1:-67881769 MsaT004289.1:CDS
GGTTCCATTGGCTGTGGAGT+GGG 0.539135 1:-67875938 MsaT004289.1:CDS
TCCGCTGGCAGCTGTTCCTG+AGG 0.539542 1:-67876367 MsaT004289.1:CDS
GGTGATGAAACTGGTTCCAT+TGG 0.539624 1:-67875950 MsaT004289.1:CDS
TGGGCAAAACCAATCCTCCT+CGG 0.539703 1:+67875874 None:intergenic
GAAGTTAGAAGCAGTTGACC+AGG 0.539770 1:-67878137 MsaT004289.1:CDS
TGAGTGTTCATCACCAGGAA+AGG 0.542043 1:-67878611 MsaT004289.1:CDS
CAGGTGATCAAGCGTTTGAA+AGG 0.545429 1:-67877578 MsaT004289.1:CDS
TTCACATTGATCTACTACCC+TGG 0.547336 1:+67879200 None:intergenic
GAAAGTGGTTCCGTTGGCTG+TGG 0.549655 1:-67876420 MsaT004289.1:CDS
TTCTAACTTCTCTTCTGCTG+AGG 0.549668 1:+67878150 None:intergenic
TAAGGCAGAAGAGATTATCA+AGG 0.551599 1:-67876062 MsaT004289.1:CDS
TGTGTTGCTGCGAGAGAGGA+TGG 0.552898 1:-67883679 MsaT004289.1:CDS
TTGCCAAGTATGTGGATCCC+TGG 0.553257 1:-67876475 MsaT004289.1:CDS
TAAGGCAGAAGAGATTATTG+AGG 0.553441 1:-67876547 MsaT004289.1:CDS
ACAGAGAAAGGAGTTTCAGC+AGG 0.553933 1:-67877597 MsaT004289.1:CDS
CTTATCAAATGCCAATGTGT+TGG 0.554180 1:+67878807 None:intergenic
TTGGGTTCTAGACCCAGAAA+AGG 0.554581 1:-67878478 MsaT004289.1:CDS
GGAGATGTCATGCTGATTTG+TGG 0.554732 1:-67876447 MsaT004289.1:CDS
CGACTGCTGCGATCCTCCGC+TGG 0.556838 1:-67876382 MsaT004289.1:CDS
ATGTCAATATCCTGGAAGCC+AGG 0.557352 1:+67879399 None:intergenic
TAAGCCGTTGGTCGCAAACA+AGG 0.557591 1:-67881599 MsaT004289.1:CDS
GACTATGCCATAGTTAGCCC+TGG 0.558059 1:-67876738 MsaT004289.1:CDS
AAATTGTCATCACGCAGATT+AGG 0.560313 1:+67879345 None:intergenic
GGTACCCACTCCACAGCCAA+TGG 0.560665 1:+67875934 None:intergenic
GGGTGCATTGTCTTCATCAA+AGG 0.560961 1:-67875801 MsaT004289.1:CDS
CATGACATCTCCTCTTTCCA+GGG 0.561920 1:+67876458 None:intergenic
TTGCCAAGTATGTGGATCGC+GGG 0.563072 1:-67875999 MsaT004289.1:CDS
TCCTCAGGAACAGCTGCCAG+CGG 0.564075 1:+67876366 None:intergenic
GCTGAAACTCCTTTCTCTGT+AGG 0.564808 1:+67877600 None:intergenic
TAATGGCGGTGGAGTTGATG+CGG 0.564836 1:-67883850 MsaT004289.1:CDS
AGAAACTGGTGTTATTCAAG+AGG 0.565350 1:-67879085 MsaT004289.1:CDS
AGACCGCTTAAAGATTTGGA+TGG 0.566779 1:-67881822 MsaT004289.1:CDS
GGTTCCGTTGGCTGTGGAGT+GGG 0.567218 1:-67876414 MsaT004289.1:CDS
GTAACCTTGTTTGCGACCAA+CGG 0.570120 1:+67881595 None:intergenic
CAAGGAGGTCATTCCTCATG+AGG 0.570635 1:-67876044 MsaT004289.1:CDS
TCAGGAACAGCTGCCAGCGG+AGG 0.571501 1:+67876369 None:intergenic
TGAGGCGGAAGATTCCAAGC+TGG 0.572135 1:-67879760 MsaT004289.1:CDS
AGTAGAGTTGAGAATGGCTC+CGG 0.573758 1:-67878209 MsaT004289.1:CDS
GGGTGCATTGTCTTCATCCA+AGG 0.576286 1:-67876271 MsaT004289.1:CDS
ACTATAAGCAAAAGTCCCCT+AGG 0.576594 1:-67879720 MsaT004289.1:CDS
AATAGCAACTTCTGGTGTGA+AGG 0.576721 1:-67878368 MsaT004289.1:CDS
CATCTTGAGTGTTCATCACC+AGG 0.578167 1:-67878616 MsaT004289.1:CDS
GCCACTTAAAATAAAACATG+TGG 0.579555 1:+67877628 None:intergenic
CTGAAAACAAATATAACAAG+AGG 0.579867 1:-67880487 MsaT004289.1:intron
CTGATTTGTGGTGATGAAAG+TGG 0.580324 1:-67876435 MsaT004289.1:CDS
ATGTGAAGTAGAGTTGAGAA+TGG 0.580970 1:-67878215 MsaT004289.1:CDS
GTATGTGGATCCCTGGAAAG+AGG 0.581862 1:-67876468 MsaT004289.1:CDS
TAAAGCTAGTTAATCACCGT+TGG 0.584487 1:-67880682 MsaT004289.1:CDS
GAAACTGGTTCCATTGGCTG+TGG 0.584504 1:-67875944 MsaT004289.1:CDS
CTTGAGAGACACGTACTGTA+CGG 0.584559 1:-67876612 MsaT004289.1:CDS
CGGCTGTTGAAAGAAAGAAC+AGG 0.584901 1:-67877206 MsaT004289.1:CDS
AGTGAGTATGCAGAAGATGC+TGG 0.586456 1:-67878286 MsaT004289.1:CDS
AATGCCTGTTGATGCTACCT+CGG 0.587295 1:-67883608 MsaT004289.1:intron
TTACCTTCCTGATGCAGTAG+CGG 0.587895 1:+67877466 None:intergenic
CTAACAAATGGTAAATCTCA+AGG 0.589314 1:-67878949 MsaT004289.1:CDS
GATATAGCTTGCCAAGTATG+TGG 0.590227 1:-67876483 MsaT004289.1:CDS
TCCAAGCTGGGGCAATCAGG+GGG 0.590436 1:-67879747 MsaT004289.1:CDS
GAGTTCTCACTTATATCAGA+AGG 0.591107 1:+67877854 None:intergenic
CGATTCAAGCTTGTTAATGG+CGG 0.592256 1:-67883864 MsaT004289.1:CDS
ACTCCTTTCTCTGTAGGCGA+TGG 0.592552 1:+67877606 None:intergenic
GCTCAAGGGACAGACTCACT+TGG 0.593309 1:+67875778 None:intergenic
TTGAAAGAAAGAACAGGTCA+TGG 0.594190 1:-67877200 MsaT004289.1:CDS
TACTCGCTTTCTTGATACTG+GGG 0.595273 1:-67876763 MsaT004289.1:CDS
ATATGGAGGCTCCAAGAAAG+TGG 0.595831 1:-67877229 MsaT004289.1:CDS
AGCTTCTGCCTCCAACACAT+TGG 0.597432 1:-67878818 MsaT004289.1:CDS
GATGTAGCTTGCCAAGTATG+TGG 0.597538 1:-67876007 MsaT004289.1:CDS
GAGGTTGAGTCTAACTCTGA+AGG 0.599018 1:+67878049 None:intergenic
TTCCAAGCTGGGGCAATCAG+GGG 0.599814 1:-67879748 MsaT004289.1:CDS
AGATGTTGGTGACTGCATTG+TGG 0.600644 1:-67883654 MsaT004289.1:CDS
GCTGGCAGCTGTTCCTGAGG+AGG 0.600700 1:-67876364 MsaT004289.1:CDS
GTCAAAATCTCTAAGAGAGG+AGG 0.602945 1:-67879664 MsaT004289.1:CDS
TGGACCAGTAGAATTTGGGA+TGG 0.603738 1:+67879632 None:intergenic
TAGTGGTGAGCCTGATGCAA+CGG 0.604279 1:-67878692 MsaT004289.1:CDS
TGGTTCCGTTGGCTGTGGAG+TGG 0.604568 1:-67876415 MsaT004289.1:CDS
CAAGGTAGAAAAGGCAAGTG+AGG 0.606134 1:-67879142 MsaT004289.1:CDS
GATCAAGCGTTTGAAAGGAA+GGG 0.612539 1:-67877573 MsaT004289.1:CDS
TGCGGATTTAGAAACCAAGC+AGG 0.612594 1:+67878840 None:intergenic
GTTGAACATAGTGCTGATGT+TGG 0.612914 1:-67883631 MsaT004289.1:CDS
AAATCCCAATTATCAAGACC+AGG 0.613763 1:+67878568 None:intergenic
TTGGCAGCTGACTTCTTGCG+CGG 0.614710 1:+67878508 None:intergenic
AGTACCCACTCCACAGCCAA+CGG 0.616922 1:+67876410 None:intergenic
TCATCGTCGCGATCCTCATG+AGG 0.617058 1:+67876513 None:intergenic
AATCGACACGTGGCATGCCA+GGG 0.619056 1:+67876721 None:intergenic
TGTGTTGGAGGCAGAAGCTG+CGG 0.620092 1:+67878822 None:intergenic
TTGAGTACGTCTGCAAGCCG+GGG 0.621599 1:-67876641 MsaT004289.1:CDS
GTACAGTACGTGTCTCTCAA+GGG 0.622673 1:+67876614 None:intergenic
GGTGGCTCATCAACCACAGA+TGG 0.622968 1:+67878916 None:intergenic
GGTGATGAAAGTGGTTCCGT+TGG 0.623291 1:-67876426 MsaT004289.1:CDS
GGTGTGTGTTGCTGCGAGAG+AGG 0.626024 1:-67883683 MsaT004289.1:CDS
TCATCATTGCAACCCTCATG+AGG 0.626300 1:+67876031 None:intergenic
AAGGAGGTCATTCCTCATGA+GGG 0.627795 1:-67876043 MsaT004289.1:CDS
AGACCAAATGATGAAGAGCA+TGG 0.627980 1:-67877779 MsaT004289.1:CDS
TGGTTCCATTGGCTGTGGAG+TGG 0.628638 1:-67875939 MsaT004289.1:CDS
GCATAATATTAGCAACCTGA+AGG 0.628689 1:-67879526 MsaT004289.1:CDS
AAGTAGTCCGCTACAACACA+AGG 0.628899 1:+67876666 None:intergenic
TCAAGAAAGCGAGTATAAGG+AGG 0.629991 1:+67876771 None:intergenic
TCCAAATGAGACAAGAGATG+AGG 0.630653 1:-67877915 MsaT004289.1:CDS
TTGCCGCTACTGCATCAGGA+AGG 0.631958 1:-67877469 MsaT004289.1:intron
ATACAAATTCATCTGGTCTG+TGG 0.632592 1:-67879368 MsaT004289.1:CDS
ATCGGAAAAGGCAGTATTTG+GGG 0.632673 1:-67877945 MsaT004289.1:CDS
TTGTTAACATCAAGGATTTG+GGG 0.635516 1:+67878331 None:intergenic
GCACATTCAGATGAAAAGCT+CGG 0.636983 1:-67879267 MsaT004289.1:CDS
ATCGTCAAAATCTCTAAGAG+AGG 0.640134 1:-67879667 MsaT004289.1:CDS
GGAAGAGGCTAAGGATTCTG+AGG 0.640663 1:-67879784 MsaT004289.1:CDS
TCCTCGGGAATAGCTGTCAG+CGG 0.641403 1:+67875890 None:intergenic
TTTGCCGTGATTCTCATCAG+TGG 0.642588 1:-67877550 MsaT004289.1:CDS
CTTGCCAAGTATGTGGATCG+CGG 0.642622 1:-67876000 MsaT004289.1:CDS
GGCAGGAAAATTATTGCCAG+AGG 0.644253 1:-67877300 MsaT004289.1:CDS
ATGAGACAAGAGATGAGGCA+GGG 0.645233 1:-67877910 MsaT004289.1:CDS
TCGTCGCGATCCTCATGAGG+AGG 0.645323 1:+67876516 None:intergenic
GAAAGAAAGCCATCAGACAC+AGG 0.645551 1:+67878895 None:intergenic
ACAGGATACTCTTAAGCGTG+CGG 0.647401 1:-67876829 MsaT004289.1:intron
ATTCCATCCAAATCTTTAAG+CGG 0.647811 1:+67881819 None:intergenic
GAGAGAGGATGGAAAGATGT+TGG 0.648494 1:-67883668 MsaT004289.1:CDS
GGAGGTTATTCCTCCTCATG+AGG 0.650168 1:-67876526 MsaT004289.1:CDS
TGAGATACCTTGTGTTGTAG+CGG 0.650742 1:-67876673 MsaT004289.1:CDS
TAAGGATTCTGAGGATGAGG+CGG 0.653839 1:-67879775 MsaT004289.1:CDS
TCTCTCGCAGCAACACACAC+CGG 0.655994 1:+67883685 None:intergenic
TCCGCTGACAGCTATTCCCG+AGG 0.660558 1:-67875891 MsaT004289.1:CDS
GATCAATGTGAAGACAGAGA+AGG 0.661317 1:-67879189 MsaT004289.1:CDS
GGCAGAAGAGATTATCAAGG+AGG 0.663325 1:-67876059 MsaT004289.1:CDS
TCGGGAATAGCTGTCAGCGG+AGG 0.663496 1:+67875893 None:intergenic
ATGACCACTGATGAGAATCA+CGG 0.663566 1:+67877546 None:intergenic
GGACGGTGCCATCAATATGG+AGG 0.665513 1:-67877243 MsaT004289.1:CDS
AGGCGGAAGATTCCAAGCTG+GGG 0.675147 1:-67879758 MsaT004289.1:CDS
AGGATCGAAAGTGCCATCTG+TGG 0.676967 1:-67878929 MsaT004289.1:CDS
ATGGCGGTGGAGTTGATGCG+GGG 0.679578 1:-67883848 MsaT004289.1:CDS
TGGAGGCTCCAAGAAAGTGG+CGG 0.683408 1:-67877226 MsaT004289.1:CDS
TGCCAGAGGAGCCTTATGAG+TGG 0.683644 1:-67877286 MsaT004289.1:CDS
TAGCTCCAGATCCTATAAGG+AGG 0.687705 1:-67877505 MsaT004289.1:CDS
CATACCAATCTTCCAGCCAA+CGG 0.689524 1:+67880666 None:intergenic
TCTTGAAACCCCTTCCACCA+AGG 0.691814 1:-67879160 MsaT004289.1:CDS
AGAAAGCCATCAGACACAGG+TGG 0.698252 1:+67878898 None:intergenic
GCTGACAGCTATTCCCGAGG+AGG 0.706393 1:-67875888 MsaT004289.1:CDS
TGGACCCACAAATCGACACG+TGG 0.713655 1:+67876711 None:intergenic
TGATGAACACTCAAGATGTG+TGG 0.746588 1:+67878621 None:intergenic
TGTCTCTCAAGGGAGAACCC+CGG 0.757252 1:+67876624 None:intergenic
GAGTATGCAGAAGATGCTGG+TGG 0.768912 1:-67878283 MsaT004289.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 67875793 67884071 67875793 ID=MsaG004289
Chr1 mRNA 67875793 67884071 67875793 ID=MsaT004289.1;Parent=MsaG004289
Chr1 exon 67875793 67876105 67875793 ID=MsaT004289.1.exon11;Parent=MsaT004289.1
Chr1 CDS 67875793 67876105 67875793 ID=cds.MsaT004289.1;Parent=MsaT004289.1
Chr1 exon 67876267 67876847 67876267 ID=MsaT004289.1.exon10;Parent=MsaT004289.1
Chr1 CDS 67876267 67876847 67876267 ID=cds.MsaT004289.1;Parent=MsaT004289.1
Chr1 exon 67877145 67877361 67877145 ID=MsaT004289.1.exon9;Parent=MsaT004289.1
Chr1 CDS 67877145 67877361 67877145 ID=cds.MsaT004289.1;Parent=MsaT004289.1
Chr1 exon 67877470 67879830 67877470 ID=MsaT004289.1.exon8;Parent=MsaT004289.1
Chr1 CDS 67877470 67879830 67877470 ID=cds.MsaT004289.1;Parent=MsaT004289.1
Chr1 exon 67880488 67880532 67880488 ID=MsaT004289.1.exon7;Parent=MsaT004289.1
Chr1 CDS 67880488 67880532 67880488 ID=cds.MsaT004289.1;Parent=MsaT004289.1
Chr1 exon 67880671 67880737 67880671 ID=MsaT004289.1.exon6;Parent=MsaT004289.1
Chr1 CDS 67880671 67880737 67880671 ID=cds.MsaT004289.1;Parent=MsaT004289.1
Chr1 exon 67881569 67881653 67881569 ID=MsaT004289.1.exon5;Parent=MsaT004289.1
Chr1 CDS 67881569 67881653 67881569 ID=cds.MsaT004289.1;Parent=MsaT004289.1
Chr1 exon 67881752 67881853 67881752 ID=MsaT004289.1.exon4;Parent=MsaT004289.1
Chr1 CDS 67881752 67881853 67881752 ID=cds.MsaT004289.1;Parent=MsaT004289.1
Chr1 exon 67883609 67883713 67883609 ID=MsaT004289.1.exon3;Parent=MsaT004289.1
Chr1 CDS 67883609 67883713 67883609 ID=cds.MsaT004289.1;Parent=MsaT004289.1
Chr1 exon 67883800 67883889 67883800 ID=MsaT004289.1.exon2;Parent=MsaT004289.1
Chr1 CDS 67883800 67883889 67883800 ID=cds.MsaT004289.1;Parent=MsaT004289.1
Chr1 exon 67884045 67884071 67884045 ID=MsaT004289.1.exon1;Parent=MsaT004289.1
Chr1 CDS 67884045 67884071 67884045 ID=cds.MsaT004289.1;Parent=MsaT004289.1
Gene Sequence

>MsaT004289.1

ATGCTCGAAACGAGTCACTCTTCGCGAATTCGATTCAAGCTTGTTAATGGCGGTGGAGTTGATGCGGGGAAGAACACTGGAAATTGCACGCATGTTATTGTTGATAAAATTGCTTATGACGATCCGGTGTGTGTTGCTGCGAGAGAGGATGGAAAGATGTTGGTGACTGCATTGTGGGTTGAACATAGTGCTGATGTTGGAATGCCTGTTGATGCTACCTCGGTCATGTACAGACCGCTTAAAGATTTGGATGGAATTCCGGGTGCAAAAGATTTGGTTGTCTGCTTGACTGGCTATCTACGCCAGGATCGGGATGACATTATGACAATGGTTGGCCTGATGGGTGCACAGTTTTCTAAGCCGTTGGTCGCAAACAAGGTTACTCATCTAATATGCTACAAATTTGAAGGGAGAAATGAGCTTGCCAAGAGATTGGCCACAATAAAGCTAGTTAATCACCGTTGGCTGGAAGATTGCTTGAAGGATTGGGTGCTTCTTCCTGAAAACAAATATAACAAGAGTGGTTTTGAACTAGAGATGATGGCGGAAGAGGCTAAGGATTCTGAGGATGAGGCGGAAGATTCCAAGCTGGGGCAATCAGGGGGAAGGACTATAAGCAAAAGTCCCCTAGGTTCAAAATTTGGCACAGCTGTGACTCATGGATCGTCAAAATCTCTAAGAGAGGAGGCATCAAAAGCCATCCCAAATTCTACTGGTCCACAAGTTTTTCCAAATGCCAATAAGGGTAAAGACTCTTCGATTACTACAGGAAATAAGAACAATTCTGATCAAGACGTAGACTTGCATAATATTAGCAACCTGAAGGTAAGCTGTCAGGTTCCTGAAGTTTCTTTGCATTCTACATCATGTCAGCTACATGAATCATATGTTAGGACCATTGAGTCTAAGAATGCTGATTTTCCAAAGGCACCTGGCTTCCAGGATATTGACATTACTGGAAATACAAATTCATCTGGTCTGTGGCCTAATCTGCGTGATGACAATTTGGAGACCAAGAAGTTAAGTTACACAAGATCAACTTCTACAAGTGCTGATGGACTTGCACATTCAGATGAAAAGCTCGGTGCTACGAGTTATTCTAGGAAGAACCAAAAAGGGTTTACCTTTTCCAGGGTAGTAGATCAATGTGAAGACAGAGAAGGCAGCAATCTTGAAACCCCTTCCACCAAGGTAGAAAAGGCAAGTGAGGTAATCAAATCTGCATGTGTTGAAGGTTCTGGGAAAGAAACTGGTGTTATTCAAGAGGATTATCAAAATAATTCATTGCCTCAAAAGAGGACAAATGAAGCTGCTTCTTCTACCAAACTAAAATCAAGAAAGATCACTAGTAATGCAAAATTGTCTATTGAAAAAACCCCACTAACAAATGGTAAATCTCAAGGATCGAAAGTGCCATCTGTGGTTGATGAGCCACCTGTGTCTGATGGCTTTCTTTCTGTTGACAAAGATGGTAGAAATAACTTAAATACCTGCTTGGTTTCTAAATCCGCAGCTTCTGCCTCCAACACATTGGCATTTGATAAGCCTATCTCAGGAAATGCAGAATCTGCTCAACGTGATAATGCTTATCAGAATTCAGCACAAAAGACTGTTCAGAGCTTAATCAAGTCTAAAGATAGTGGTGAGCCTGATGCAACGGTTTTTGGAAAGGGGCAAGGTGATAACGAAGCAGAGCAACTTAATATCACCACACATCTTGAGTGTTCATCACCAGGAAAGGATTCAAAGAATGAAGGATTTCCTGGTCTTGATAATTGGGATTTAAGCAATGAAGAAAGCAATAAGCCGATTAGAAAGTCACCGCGCAAGAAGTCAGCTGCCAAGAGAACTTTGGGTTCTAGACCCAGAAAAGGTGTTACTGCTAAACTGAAAAGTTCAGTTTGCTTGAATAAAACTACTCAGCGAGATGAAGGTGTAAGTTTTTCCAGCAGAAGCAAAGAAATAGCAACTTCTGGTGTGAAGGAGCGTCACGCATCACCCCAAATCCTTGATGTTAACAAATTGATGGAGCAAAAACCGGTTAGTGAGTATGCAGAAGATGCTGGTGGCAGAACTGATTTTTTGGATGACGAAACTGAGGCTCCAGATGACAAATGTGAAGTAGAGTTGAGAATGGCTCCGGATGAAGAATTGGTTCATCTTTCAAATAAAGTAGACACCTCAGCAGAAGAGAAGTTAGAAGCAGTTGACCAGGATAAAAAATGTGAAGAAGCAATGCCTCCTAAAAAAGTCACAAATGAAACTAAAAAACAGAAATTACCTTCAGAGTTAGACTCAACCTCGAAGTTAAAAGTAAAACATCAAACGATAAAACGTCCTGCAAGTAAAACCAAGAAGACCACAGCTGCAAAGAAGTTAGCGAAATCGGAAAAGGCAGTATTTGGGGAGAAGATTCCAAATGAGACAAGAGATGAGGCAGGGATAAAAATCCTGAAAGAAATGTCACTGTCTGTACCTTCTGATATAAGTGAGAACTCAAATGCACCAAAAAATAAGCTCGAGAATTTTATTGAAGAGGAAAAGGAGAATAGACCAAATGATGAAGAGCATGGTTTTGAGGAGAGAAGAAATGTTGGGAATCGAACTACTAAATCTAGTGCTAAGCCAGCAAATATCAAGTCTAAGGAAATGAAACACGCTCCATCTATATCAGAATTTAAGGCAAGAGTAAAACCTGAAACCACATGTTTTATTTTAAGTGGCCATCGCCTACAGAGAAAGGAGTTTCAGCAGGTGATCAAGCGTTTGAAAGGAAGGGTTTGCCGTGATTCTCATCAGTGGTCATATCAGGCAACACACTTCATAGCTCCAGATCCTATAAGGAGGACAGAGAAATTTTTTGCCGCTACTGCATCAGGAAGGTGGATTCTTAAAACTGATTTCTTAAGTGCCAGTAGCCAGGCAGGAAAATTATTGCCAGAGGAGCCTTATGAGTGGCACAAAAATGGTCTCAGCGAGGACGGTGCCATCAATATGGAGGCTCCAAGAAAGTGGCGGCTGTTGAAAGAAAGAACAGGTCATGGTGCCTTCTATGGGATGCGCATTGTTGTATATGGTGATTGTTTTGCTCCACCTTTGGATACTCTTAAGCGTGCGGTCAAAGCTGGAGATGGAACAATATTAGCAACATCTCCTCCTTATACTCGCTTTCTTGATACTGGGGTCGACTATGCCATAGTTAGCCCTGGCATGCCACGTGTCGATTTGTGGGTCCAAGAGTTCTTAAAACATGAGATACCTTGTGTTGTAGCGGACTACTTGGTTGAGTACGTCTGCAAGCCGGGGTTCTCCCTTGAGAGACACGTACTGTACGGCACTCATGCTTTGGCAGATAGGTCATTTGCAAAACTTCAAAGTAAGGCAGAAGAGATTATTGAGGAGGTTATTCCTCCTCATGAGGATCGCGACGATGACAATGATGATATAGCTTGCCAAGTATGTGGATCCCTGGAAAGAGGAGATGTCATGCTGATTTGTGGTGATGAAAGTGGTTCCGTTGGCTGTGGAGTGGGTACTCATATCGACTGCTGCGATCCTCCGCTGGCAGCTGTTCCTGAGGAGGATTGGTTTTGCCCAAAATGCAGTAGCACCCAAACCCAAACATGCTCAAAAAAACCCAATAAAAGGAAAAAGGGTGCATTGTCTTCATCCAAGGCAAAGTCATTTACCAAACATCAAAGTAAGGCAGAAGAGATTATCAAGGAGGTCATTCCTCATGAGGGTTGCAATGATGACGATGTAGCTTGCCAAGTATGTGGATCGCGGGAAAGAGGAGATTTGATGCTGATTTGTGGTGATGAAACTGGTTCCATTGGCTGTGGAGTGGGTACCCATATCGATTGCTGCAATCCTCCGCTGACAGCTATTCCCGAGGAGGATTGGTTTTGCCCAAAATGCATTAGAATCCAAAAATGCTCAAAAAAAGTCAATAAAAGTAAAAAGGGTGCATTGTCTTCATCAAAGGCCAAGTGA
Protein sequence

>MsaT004289.1

MLETSHSSRIRFKLVNGGGVDAGKNTGNCTHVIVDKIAYDDPVCVAAREDGKMLVTALWVEHSADVGMPVDATSVMYRPLKDLDGIPGAKDLVVCLTGYLRQDRDDIMTMVGLMGAQFSKPLVANKVTHLICYKFEGRNELAKRLATIKLVNHRWLEDCLKDWVLLPENKYNKSGFELEMMAEEAKDSEDEAEDSKLGQSGGRTISKSPLGSKFGTAVTHGSSKSLREEASKAIPNSTGPQVFPNANKGKDSSITTGNKNNSDQDVDLHNISNLKVSCQVPEVSLHSTSCQLHESYVRTIESKNADFPKAPGFQDIDITGNTNSSGLWPNLRDDNLETKKLSYTRSTSTSADGLAHSDEKLGATSYSRKNQKGFTFSRVVDQCEDREGSNLETPSTKVEKASEVIKSACVEGSGKETGVIQEDYQNNSLPQKRTNEAASSTKLKSRKITSNAKLSIEKTPLTNGKSQGSKVPSVVDEPPVSDGFLSVDKDGRNNLNTCLVSKSAASASNTLAFDKPISGNAESAQRDNAYQNSAQKTVQSLIKSKDSGEPDATVFGKGQGDNEAEQLNITTHLECSSPGKDSKNEGFPGLDNWDLSNEESNKPIRKSPRKKSAAKRTLGSRPRKGVTAKLKSSVCLNKTTQRDEGVSFSSRSKEIATSGVKERHASPQILDVNKLMEQKPVSEYAEDAGGRTDFLDDETEAPDDKCEVELRMAPDEELVHLSNKVDTSAEEKLEAVDQDKKCEEAMPPKKVTNETKKQKLPSELDSTSKLKVKHQTIKRPASKTKKTTAAKKLAKSEKAVFGEKIPNETRDEAGIKILKEMSLSVPSDISENSNAPKNKLENFIEEEKENRPNDEEHGFEERRNVGNRTTKSSAKPANIKSKEMKHAPSISEFKARVKPETTCFILSGHRLQRKEFQQVIKRLKGRVCRDSHQWSYQATHFIAPDPIRRTEKFFAATASGRWILKTDFLSASSQAGKLLPEEPYEWHKNGLSEDGAINMEAPRKWRLLKERTGHGAFYGMRIVVYGDCFAPPLDTLKRAVKAGDGTILATSPPYTRFLDTGVDYAIVSPGMPRVDLWVQEFLKHEIPCVVADYLVEYVCKPGFSLERHVLYGTHALADRSFAKLQSKAEEIIEEVIPPHEDRDDDNDDIACQVCGSLERGDVMLICGDESGSVGCGVGTHIDCCDPPLAAVPEEDWFCPKCSSTQTQTCSKKPNKRKKGALSSSKAKSFTKHQSKAEEIIKEVIPHEGCNDDDVACQVCGSRERGDLMLICGDETGSIGCGVGTHIDCCNPPLTAIPEEDWFCPKCIRIQKCSKKVNKSKKGALSSSKAK*