AlfalfaGEDB | MsaG004369

M. sativa ssp. caerulea / MsaG004369

KEGG: K08065
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG004369	XP_013469195.1	88.660	194	13	2	1	185	1	194	1.91e-114	333

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG004369	sp\|O82248\|NFYB5_ARATH	62.121	132	35	1	16	132	18	149	3.51e-57	180

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification
MsaG004369	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000402	MsaG004369	0.810167	2.254599e-50	3.865885e-48
MsaG004369	MsaG006729	0.807089	1.017587e-49	1.620276e-47
MsaG004369	MsaG007092	0.847362	2.290329e-59	1.135206e-56
MsaG004369	MsaG012163	0.808681	4.682165e-50	7.745883e-48
MsaG004369	MsaG016778	0.800865	1.973910e-48	2.721469e-46
MsaG004369	MsaG018714	0.810375	2.034484e-50	3.506313e-48
MsaG004369	MsaG026607	0.806750	1.198983e-49	1.894009e-47
MsaG004369	MsaG029211	0.825134	9.813990e-54	2.477812e-51
MsaG004369	MsaG035768	0.806557	1.316300e-49	2.069879e-47
MsaG004369	MsaG036809	0.808749	4.528933e-50	7.504697e-48
MsaG004369	MsaG046155	0.817798	4.765267e-52	9.894394e-50
MsaG004369	MsaG046696	0.813217	4.930861e-51	9.114392e-49
MsaG004369	MsaG001786	0.803533	5.608630e-49	8.218883e-47
MsaG004369	MsaG013755	0.812524	6.980330e-51	1.268196e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG004369	MtrunA17_Chr1g0195851	88.660	194	13	2	1	185	1	194	1.75e-118	333
MsaG004369	MtrunA17_Chr2g0296321	64.865	148	47	3	6	152	38	181	3.02e-59	185
MsaG004369	MtrunA17_Chr4g0067091	79.412	102	21	0	24	125	12	113	1.11e-58	182
MsaG004369	MtrunA17_Chr1g0191981	74.000	100	26	0	34	133	4	103	5.27e-53	165
MsaG004369	MtrunA17_Chr4g0076321	64.407	118	39	1	35	152	20	134	1.18e-52	164
MsaG004369	MtrunA17_Chr2g0290491	64.407	118	36	1	33	150	16	127	7.65e-52	164
MsaG004369	MtrunA17_Chr8g0384451	72.277	101	28	0	33	133	39	139	1.40e-51	164
MsaG004369	MtrunA17_Chr7g0263601	62.162	111	42	0	32	142	24	134	1.08e-50	160
MsaG004369	MtrunA17_Chr3g0102351	60.504	119	47	0	33	151	26	144	1.32e-50	161
MsaG004369	MtrunA17_Chr5g0446491	71.875	96	27	0	33	128	22	117	9.63e-50	159
MsaG004369	MtrunA17_Chr8g0382931	71.429	98	28	0	33	130	27	124	4.89e-49	157
MsaG004369	MtrunA17_Chr1g0185911	70.526	95	28	0	33	127	28	122	2.19e-48	155
MsaG004369	MtrunA17_Chr4g0076381	63.462	104	37	1	26	128	49	152	4.23e-48	156
MsaG004369	MtrunA17_Chr1g0165041	56.604	106	46	0	32	137	4	109	1.30e-42	141
MsaG004369	MtrunA17_Chr1g0159271	43.956	91	51	0	48	138	1	91	1.34e-22	87.4
MsaG004369	MtrunA17_Chr5g0446601	44.565	92	51	0	31	122	21	112	3.36e-21	85.9
MsaG004369	MtrunA17_Chr1g0159291	40.000	80	48	0	39	118	7	86	1.57e-18	76.3
MsaG004369	MtrunA17_Chr2g0307551	37.179	78	49	0	35	112	12	89	2.65e-17	75.1
MsaG004369	MtrunA17_Chr1g0158951	46.875	64	34	0	64	127	19	82	2.99e-16	70.9
MsaG004369	MtrunA17_Chr4g0062271	32.051	78	53	0	35	112	12	89	2.83e-13	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG004369	AT2G47810.1	62.121	132	35	1	16	132	18	149	3.57e-58	180
MsaG004369	AT1G09030.1	57.143	140	56	1	32	171	1	136	7.73e-53	166
MsaG004369	AT5G47640.1	68.468	111	32	1	33	140	26	136	2.17e-52	166
MsaG004369	AT4G14540.1	75.000	96	24	0	33	128	20	115	5.84e-52	164
MsaG004369	AT2G13570.1	73.267	101	27	0	33	133	35	135	2.75e-51	164
MsaG004369	AT2G38880.3	64.865	111	38	1	32	141	19	129	3.30e-51	161
MsaG004369	AT2G38880.2	64.865	111	38	1	32	141	19	129	4.01e-51	161
MsaG004369	AT2G38880.1	64.865	111	38	1	32	141	19	129	4.01e-51	161
MsaG004369	AT2G38880.5	64.865	111	38	1	32	141	19	129	4.01e-51	161
MsaG004369	AT3G53340.5	50.336	149	67	1	32	180	27	168	6.33e-51	162
MsaG004369	AT3G53340.1	50.336	149	67	1	32	180	27	168	6.33e-51	162
MsaG004369	AT2G38880.7	65.455	110	37	1	32	141	19	127	3.38e-50	159
MsaG004369	AT2G37060.2	63.063	111	41	0	32	142	28	138	4.94e-50	159
MsaG004369	AT2G37060.3	63.063	111	41	0	32	142	28	138	4.94e-50	159
MsaG004369	AT2G37060.1	63.063	111	41	0	32	142	28	138	4.94e-50	159
MsaG004369	AT2G38880.4	73.118	93	25	0	32	124	19	111	2.28e-49	155
MsaG004369	AT2G38880.11	73.118	93	25	0	32	124	19	111	6.37e-49	156
MsaG004369	AT2G38880.8	73.118	93	25	0	32	124	19	111	6.37e-49	156
MsaG004369	AT3G53340.4	72.043	93	26	0	32	124	27	119	7.04e-49	155
MsaG004369	AT3G53340.3	69.697	99	29	1	32	130	27	124	9.14e-49	155
MsaG004369	AT3G53340.2	69.697	99	29	1	32	130	27	124	9.14e-49	155
MsaG004369	AT2G38880.9	73.118	93	25	0	32	124	19	111	9.31e-49	155
MsaG004369	AT2G38880.10	73.118	93	25	0	32	124	19	111	9.31e-49	155
MsaG004369	AT2G38880.6	73.118	93	25	0	32	124	19	111	9.31e-49	155
MsaG004369	AT5G47670.2	62.626	99	37	0	31	129	26	124	5.97e-46	150
MsaG004369	AT5G47670.1	62.626	99	37	0	31	129	55	153	1.18e-45	150
MsaG004369	AT5G47670.3	62.626	99	37	0	31	129	55	153	1.18e-45	150
MsaG004369	AT1G21970.1	50.862	116	57	0	19	134	44	159	5.06e-41	139
MsaG004369	AT5G23090.4	35.052	97	61	1	35	129	12	108	1.36e-17	76.3
MsaG004369	AT5G23090.5	38.462	78	48	0	35	112	12	89	1.24e-16	73.6
MsaG004369	AT5G23090.2	38.462	78	48	0	35	112	12	89	1.24e-16	73.6
MsaG004369	AT5G23090.1	38.462	78	48	0	35	112	12	89	1.24e-16	73.6
MsaG004369	AT5G08190.2	32.292	96	63	1	35	128	12	107	5.19e-16	72.0
MsaG004369	AT5G08190.1	34.615	78	51	0	35	112	12	89	6.87e-15	69.3

Find 34 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTCTTCTATGTTCAATAAT+AGG	0.214525	1:-68820896	MsaT004369.1:CDS
GATGATGTTTGTTGGGCTTT+GGG	0.221205	1:-68821116	MsaT004369.1:CDS
TGATGTTGTTACCCTTATTT+TGG	0.229982	1:+68821012	None:intergenic
TGATGATGTTTGTTGGGCTT+TGG	0.269661	1:-68821117	MsaT004369.1:CDS
GGAAAGAGGGAACCAAAATA+AGG	0.312778	1:-68821024	MsaT004369.1:CDS
TGTTGGGCTTTGGGGACACT+AGG	0.347462	1:-68821107	MsaT004369.1:CDS
AAAGGTATTTGTATAAGTAT+AGG	0.352771	1:-68821058	MsaT004369.1:CDS
TTTGTATAAGTATAGGGAAT+TGG	0.359649	1:-68821051	MsaT004369.1:CDS
GAAGCAACATTCACTTTCCT+TGG	0.401544	1:+68820918	None:intergenic
ATCCGACCAACATTAGCTAT+TGG	0.403010	1:+68821284	None:intergenic
AAGGTATTTGTATAAGTATA+GGG	0.417767	1:-68821057	MsaT004369.1:CDS
GAATGGTGATGATGTTTGTT+GGG	0.429389	1:-68821123	MsaT004369.1:CDS
GAAACATATGATTATGAAGA+TGG	0.453321	1:-68820954	MsaT004369.1:CDS
ACATGGGTGATAACAACAAA+GGG	0.487792	1:-68821403	MsaT004369.1:CDS
GAAAGAGGGAACCAAAATAA+GGG	0.501997	1:-68821023	MsaT004369.1:CDS
GTTGGGCTTTGGGGACACTA+GGG	0.503961	1:-68821106	MsaT004369.1:CDS
ATGATGTTTGTTGGGCTTTG+GGG	0.505210	1:-68821115	MsaT004369.1:CDS
TGAATGGTGATGATGTTTGT+TGG	0.510123	1:-68821124	MsaT004369.1:CDS
CTCTGAGACACACTCTTGCA+TGG	0.513185	1:+68821208	None:intergenic
GAGAAGCGCAAGACAGTGAA+TGG	0.520201	1:-68821140	MsaT004369.1:CDS
AGGGAATTGGATGCGGAAAG+AGG	0.523773	1:-68821038	MsaT004369.1:CDS
TTGCTTCCAATAGCTAATGT+TGG	0.538809	1:-68821290	MsaT004369.1:CDS
TTCAAAATCCCCTTGACTGA+AGG	0.540445	1:+68820865	None:intergenic
GGGAATTGGATGCGGAAAGA+GGG	0.541973	1:-68821037	MsaT004369.1:CDS
CCTATTATTGAACATAGAAG+AGG	0.550260	1:+68820896	None:intergenic
TAAGTATAGGGAATTGGATG+CGG	0.557603	1:-68821045	MsaT004369.1:CDS
ATTACGCTGAGCCACTCAAA+AGG	0.563009	1:-68821076	MsaT004369.1:CDS
GAACAGTGACCTTCAGTCAA+GGG	0.574883	1:-68820874	MsaT004369.1:CDS
TTCCAATAGCTAATGTTGGT+CGG	0.585707	1:-68821286	MsaT004369.1:CDS
ATGGTAAGTCACAAACTCCA+AGG	0.613914	1:-68820935	MsaT004369.1:CDS
AACAGTGACCTTCAGTCAAG+GGG	0.614235	1:-68820873	MsaT004369.1:CDS
AACATGGGTGATAACAACAA+AGG	0.619511	1:-68821404	MsaT004369.1:CDS
GGAACAGTGACCTTCAGTCA+AGG	0.622121	1:-68820875	MsaT004369.1:CDS
AGCATCTGACAAGTGTCACA+AGG	0.642405	1:-68821162	MsaT004369.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	68820866	68821423	68820866	ID=MsaG004369
Chr1	mRNA	68820866	68821423	68820866	ID=MsaT004369.1;Parent=MsaG004369
Chr1	exon	68820866	68821423	68820866	ID=MsaT004369.1.exon1;Parent=MsaT004369.1
Chr1	CDS	68820866	68821423	68820866	ID=cds.MsaT004369.1;Parent=MsaT004369.1

Gene Sequence

>MsaT004369.1

ATGGGTGATAACAACAAAGGGAAACTAGTCTCAGATAGAGAAGTCTTTAAGTACAACACAAATTTCTCATCTCATGAGCAAGATCATGAAGTAATCAAAGAGCAAGATCGTTTGCTTCCAATAGCTAATGTTGGTCGGATCATGAAACAAATCTTGCCACCAAATGCAAAAATCTCTAAAGATGCTAAAGAAACCATGCAAGAGTGTGTCTCAGAGTTTGTTAGTTTTGTAACTGGTGAAGCATCTGACAAGTGTCACAAGGAGAAGCGCAAGACAGTGAATGGTGATGATGTTTGTTGGGCTTTGGGGACACTAGGGTTTGATGATTACGCTGAGCCACTCAAAAGGTATTTGTATAAGTATAGGGAATTGGATGCGGAAAGAGGGAACCAAAATAAGGGTAACAACATCAACAACAACAGTGATGAAATTGAAAACAATATTAATGAAACATATGATTATGAAGATGGTAAGTCACAAACTCCAAGGAAAGTGAATGTTGCTTCCTCTTCTATGTTCAATAATAGGAACAGTGACCTTCAGTCAAGGGGATTTTGA

Protein sequence

>MsaT004369.1

MGDNNKGKLVSDREVFKYNTNFSSHEQDHEVIKEQDRLLPIANVGRIMKQILPPNAKISKDAKETMQECVSEFVSFVTGEASDKCHKEKRKTVNGDDVCWALGTLGFDDYAEPLKRYLYKYRELDAERGNQNKGNNINNNSDEIENNINETYDYEDGKSQTPRKVNVASSSMFNNRNSDLQSRGF*