Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004409 | AES61869.1 | 68.794 | 141 | 21 | 2 | 12 | 129 | 5 | 145 | 5.58e-58 | 186 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004409 | sp|Q8W4H1|GAT26_ARATH | 60.465 | 43 | 17 | 0 | 3 | 45 | 5 | 47 | 1.17e-13 | 69.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| MsaG004409 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004409 | MtrunA17_Chr4g0055611 | 62.791 | 43 | 16 | 0 | 3 | 45 | 5 | 47 | 3.26e-15 | 70.9 |
| MsaG004409 | MtrunA17_Chr5g0399781 | 62.791 | 43 | 16 | 0 | 3 | 45 | 5 | 47 | 5.12e-15 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004409 | AT4G17570.1 | 60.465 | 43 | 17 | 0 | 3 | 45 | 5 | 47 | 1.19e-14 | 69.7 |
| MsaG004409 | AT4G17570.3 | 60.465 | 43 | 17 | 0 | 3 | 45 | 5 | 47 | 1.26e-14 | 69.3 |
| MsaG004409 | AT5G47140.1 | 58.140 | 43 | 18 | 0 | 3 | 45 | 5 | 47 | 3.76e-14 | 68.2 |
Find 28 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAGTCTTCAAGGGTTCCTT+TGG | 0.332237 | 1:+69422639 | None:intergenic |
| TCAAACAACAAAATATTATC+TGG | 0.350768 | 1:+69420717 | None:intergenic |
| CACAAGATTGGCTTCTCATA+TGG | 0.363409 | 1:+69422688 | None:intergenic |
| TTTCAACAGAAACTGAAGTT+TGG | 0.364600 | 1:-69422720 | MsaT004409.1:intron |
| ATACTTTGCTGAGCTATCTT+CGG | 0.400089 | 1:+69422613 | None:intergenic |
| GACTATGAGGCCGACACTAA+TGG | 0.401191 | 1:-69420820 | MsaT004409.1:CDS |
| TACTTTGCTGAGCTATCTTC+GGG | 0.431538 | 1:+69422614 | None:intergenic |
| CCAAGTAATGAGATCGGCCT+TGG | 0.442800 | 1:-69420675 | MsaT004409.1:CDS |
| CCACAGGCGTTACACAAGAT+TGG | 0.446240 | 1:+69422676 | None:intergenic |
| TGTGTGCATTGTGGAATTAA+TGG | 0.447647 | 1:-69423899 | MsaT004409.1:intron |
| TCTTCGGGAGATAGTCTTCA+AGG | 0.462167 | 1:+69422629 | None:intergenic |
| CCAAGGCCGATCTCATTACT+TGG | 0.469229 | 1:+69420675 | None:intergenic |
| CGCCTGTGGATTGCATTTCA+AGG | 0.472275 | 1:-69422662 | MsaT004409.1:CDS |
| GAAACTGAAGTTTGGCGTAA+AGG | 0.486401 | 1:-69422712 | MsaT004409.1:CDS |
| GGCCTTGAAATGCAATCCAC+AGG | 0.512475 | 1:+69422660 | None:intergenic |
| GTCAGTGTCTCCATTAGTGT+CGG | 0.538511 | 1:+69420810 | None:intergenic |
| AAATTACGATATTCTATGGC+GGG | 0.547633 | 1:-69423926 | None:intergenic |
| ATTAGTGTCGGCCTCATAGT+CGG | 0.574226 | 1:+69420822 | None:intergenic |
| AGAGGTCGGCACCGACTATG+AGG | 0.586753 | 1:-69420833 | MsaT004409.1:CDS |
| TTAATTCCACAATGCACACA+TGG | 0.587961 | 1:+69423902 | None:intergenic |
| AATGGAGACACTGACACCAA+TGG | 0.595378 | 1:-69420802 | MsaT004409.1:CDS |
| TTTATACCAAGTAATGAGAT+CGG | 0.595926 | 1:-69420681 | MsaT004409.1:CDS |
| CCAATCTTGTGTAACGCCTG+TGG | 0.600058 | 1:-69422676 | MsaT004409.1:CDS |
| CTTCGGGAGATAGTCTTCAA+GGG | 0.601274 | 1:+69422630 | None:intergenic |
| TCTTTAGAAGAGTGCAGCCA+AGG | 0.606342 | 1:+69420658 | None:intergenic |
| GGATTGCATTTCAAGGCCAA+AGG | 0.606742 | 1:-69422655 | MsaT004409.1:CDS |
| GCGGGTCCATGTGTGCATTG+TGG | 0.639689 | 1:-69423908 | MsaT004409.1:CDS |
| GGAGACACTGACACCAATGG+AGG | 0.696756 | 1:-69420799 | MsaT004409.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 69420617 | 69423933 | 69420617 | ID=MsaG004409 |
| Chr1 | mRNA | 69420617 | 69423933 | 69420617 | ID=MsaT004409.1;Parent=MsaG004409 |
| Chr1 | exon | 69420617 | 69420855 | 69420617 | ID=MsaT004409.1.exon3;Parent=MsaT004409.1 |
| Chr1 | CDS | 69420617 | 69420855 | 69420617 | ID=cds.MsaT004409.1;Parent=MsaT004409.1 |
| Chr1 | exon | 69422617 | 69422733 | 69422617 | ID=MsaT004409.1.exon2;Parent=MsaT004409.1 |
| Chr1 | CDS | 69422617 | 69422733 | 69422617 | ID=cds.MsaT004409.1;Parent=MsaT004409.1 |
| Chr1 | exon | 69423900 | 69423933 | 69423900 | ID=MsaT004409.1.exon1;Parent=MsaT004409.1 |
| Chr1 | CDS | 69423900 | 69423933 | 69423900 | ID=cds.MsaT004409.1;Parent=MsaT004409.1 |
| Gene Sequence |
| Protein sequence |
