AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004485

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004485 MtrunA17_Chr1g0197321 88.535 157 17 1 1 156 1 157 2.58e-99 282
MsaG004485 MtrunA17_Chr1g0197261 72.436 156 43 0 1 156 1 156 4.39e-82 239
MsaG004485 MtrunA17_Chr5g0420351 55.484 155 69 0 1 155 1 155 1.27e-58 179
MsaG004485 MtrunA17_Chr1g0197461 57.143 154 66 0 3 156 2 155 9.53e-58 177
MsaG004485 MtrunA17_Chr1g0188681 52.903 155 73 0 1 155 1 155 2.41e-55 171
MsaG004485 MtrunA17_Chr1g0193311 56.129 155 68 0 1 155 1 155 5.65e-52 162
MsaG004485 MtrunA17_Chr1g0188661 52.903 155 73 0 1 155 1 155 1.51e-49 156
MsaG004485 MtrunA17_Chr4g0012761 52.258 155 74 0 1 155 1 155 3.90e-48 153
MsaG004485 MtrunA17_Chr7g0218151 50.968 155 76 0 1 155 1 155 4.80e-47 150
MsaG004485 MtrunA17_Chr1g0188651 50.968 155 74 1 1 155 1 153 8.93e-43 139
MsaG004485 MtrunA17_Chr3g0107351 43.226 155 87 1 3 156 2 156 3.61e-37 125
MsaG004485 MtrunA17_Chr3g0107341 42.581 155 88 1 3 156 2 156 8.62e-37 124
MsaG004485 MtrunA17_Chr3g0107361 41.935 155 89 1 3 156 2 156 1.86e-36 123
MsaG004485 MtrunA17_Chr3g0107331 40.645 155 91 1 3 156 2 156 5.47e-36 122
MsaG004485 MtrunA17_Chr3g0110131 41.667 156 90 1 1 155 1 156 8.19e-35 121
MsaG004485 MtrunA17_Chr4g0014321 35.443 158 100 2 1 156 1 158 1.27e-34 120
MsaG004485 MtrunA17_Chr4g0014031 42.405 158 89 2 1 156 1 158 2.41e-34 120
MsaG004485 MtrunA17_Chr3g0107381 35.714 154 98 1 1 153 1 154 1.54e-33 117
MsaG004485 MtrunA17_Chr5g0432691 38.854 157 95 1 1 156 1 157 1.24e-32 113
MsaG004485 MtrunA17_Chr4g0013041 34.320 169 96 4 1 156 1 167 1.65e-31 112
MsaG004485 MtrunA17_Chr8g0351751 34.375 160 101 2 1 156 1 160 6.52e-31 111
MsaG004485 MtrunA17_Chr2g0283941 35.256 156 99 2 1 154 1 156 1.41e-27 102
MsaG004485 MtrunA17_Chr4g0014121 40.385 156 91 2 1 154 29 184 1.79e-27 101
MsaG004485 MtrunA17_Chr2g0296831 40.496 121 71 1 1 120 1 121 1.34e-25 94.4
MsaG004485 MtrunA17_Chr4g0014131 40.506 158 92 2 1 156 1 158 1.89e-25 97.1
MsaG004485 MtrunA17_Chr3g0107461 32.075 159 102 3 1 155 1 157 6.00e-24 91.3
MsaG004485 MtrunA17_Chr2g0296861 34.737 95 61 1 1 94 1 95 2.83e-19 79.3
MsaG004485 MtrunA17_Chr4g0008701 35.185 108 69 1 3 109 6 113 1.08e-18 81.6
MsaG004485 MtrunA17_Chr7g0267601 33.333 108 71 1 3 109 6 113 1.43e-17 78.6
MsaG004485 MtrunA17_Chr4g0031421 34.109 129 77 3 29 152 1 126 3.27e-16 70.9
MsaG004485 MtrunA17_Chr3g0106421 32.308 130 84 3 29 155 1 129 4.42e-16 72.4
MsaG004485 MtrunA17_Chr3g0128721 26.452 155 113 1 3 156 6 160 6.43e-15 70.9
MsaG004485 MtrunA17_Chr3g0106501 32.824 131 84 3 29 156 1 130 7.86e-15 67.8
MsaG004485 MtrunA17_Chr3g0105791 31.250 128 82 3 29 152 1 126 1.41e-14 66.6
MsaG004485 MtrunA17_Chr1g0162261 40.426 94 55 1 64 156 22 115 2.55e-14 65.5
MsaG004485 MtrunA17_Chr6g0449861 29.518 166 98 6 1 157 9 164 1.17e-13 67.0
MsaG004485 MtrunA17_Chr6g0457851 27.381 168 99 6 1 157 11 166 2.97e-13 65.9
MsaG004485 MtrunA17_Chr1g0212151 27.439 164 100 5 1 155 1 154 1.27e-11 61.2
MsaG004485 MtrunA17_Chr3g0142191 27.778 162 96 4 1 154 1 149 4.50e-11 58.9
MsaG004485 MtrunA17_Chr1g0161931 32.990 97 64 1 61 156 25 121 5.48e-11 56.6
MsaG004485 MtrunA17_Chr1g0162181 37.234 94 58 1 64 156 22 115 9.58e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004485 AT5G48670.1 42.308 156 89 1 1 155 1 156 3.27e-31 114
MsaG004485 AT1G65300.1 37.013 154 96 1 3 155 2 155 1.21e-30 112
MsaG004485 AT3G05860.2 38.926 149 90 1 1 148 1 149 2.21e-30 109
MsaG004485 AT5G26630.1 39.744 156 93 1 1 155 1 156 2.65e-30 109
MsaG004485 AT3G05860.3 38.926 149 90 1 1 148 1 149 9.27e-30 108
MsaG004485 AT3G05860.1 38.926 149 90 1 1 148 1 149 1.31e-29 108
MsaG004485 AT1G65330.1 37.662 154 95 1 3 155 2 155 4.06e-28 105
MsaG004485 AT1G31640.1 35.333 150 95 2 3 151 2 150 7.29e-26 102
MsaG004485 AT1G22590.2 34.177 158 100 2 1 157 1 155 3.06e-25 95.1
MsaG004485 AT5G26650.1 34.641 153 99 1 4 155 2 154 6.99e-25 98.6
MsaG004485 AT5G27960.1 34.641 153 99 1 4 155 2 154 2.53e-24 96.3
MsaG004485 AT2G28700.1 40.000 110 65 1 1 109 1 110 6.36e-24 95.5
MsaG004485 AT5G06500.1 36.076 158 93 2 1 154 1 154 2.19e-22 89.7
MsaG004485 AT1G31630.1 29.801 151 104 2 3 152 2 151 4.52e-21 87.8
MsaG004485 AT5G27810.1 37.500 96 59 1 29 123 1 96 7.60e-19 77.4
MsaG004485 AT5G26580.1 30.128 156 94 3 1 155 1 142 8.60e-16 73.6
MsaG004485 AT2G40210.1 46.667 75 40 0 1 75 1 75 1.67e-14 70.1
MsaG004485 AT2G22630.1 50.000 52 26 0 1 52 1 52 2.60e-11 60.1
MsaG004485 AT2G22630.2 50.000 52 26 0 1 52 1 52 2.60e-11 60.1
MsaG004485 AT1G47760.1 30.973 113 67 3 1 110 1 105 4.79e-11 58.5

Find 35 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCTCTCAATTATCTTAT+TGG 0.120149 1:-70109108 None:intergenic
ATTATCTTATTGGCTCCTTC+TGG 0.257805 1:-70109098 None:intergenic
GATGTGGCCACACCTCTGTC+TGG 0.327640 1:-70109076 None:intergenic
GCCTTCCTTGCTGCATCATC+GGG 0.361617 1:-70108951 None:intergenic
TGCTGCATCATCGGGAATAA+AGG 0.369120 1:-70108943 None:intergenic
TCTGCATGATAAACCTTTCA+TGG 0.377114 1:-70109169 None:intergenic
TTCACCTTTCTTCTCGCCAT+TGG 0.379933 1:-70108915 None:intergenic
TGCCTTCCTTGCTGCATCAT+CGG 0.383232 1:-70108952 None:intergenic
GAGAAGATTGATACACTTGA+TGG 0.449535 1:+70109365 MsaT004485.1:CDS
TTATTGGCTCCTTCTGGATG+TGG 0.451748 1:-70109092 None:intergenic
ACTGAAGAAGTGAAGGATCT+TGG 0.461931 1:+70109308 MsaT004485.1:CDS
ACCTCTGTCTGGGAATCAAA+TGG 0.463167 1:-70109065 None:intergenic
TCAAAGCAAGGGACAAACTA+AGG 0.480536 1:+70109198 MsaT004485.1:CDS
AAGCTACAATAAAAGAAAGA+AGG 0.492405 1:+70108974 MsaT004485.1:CDS
AGCTGGAACATCACAAAGAA+TGG 0.506592 1:-70109024 None:intergenic
GCAACCAATGGCGAGAAGAA+AGG 0.507401 1:+70108911 None:intergenic
ATGTGGCCACACCTCTGTCT+GGG 0.518550 1:-70109075 None:intergenic
ATGAGAATCGTGAGCAAGAG+TGG 0.520504 1:+70109231 MsaT004485.1:CDS
CTGAAAATTATAGCACAAGC+TGG 0.522219 1:-70109041 None:intergenic
GCGTGCTGAGAAGAAGTTGA+AGG 0.552409 1:+70109334 MsaT004485.1:CDS
TCCCGATGATGCAGCAAGGA+AGG 0.554434 1:+70108950 MsaT004485.1:CDS
AAAGAAGGGTCTCATAAAGA+AGG 0.555375 1:+70108989 MsaT004485.1:CDS
TGCAGACGATTGTCAAAGCA+AGG 0.562423 1:+70109186 MsaT004485.1:CDS
AGCTACAATAAAAGAAAGAA+GGG 0.567899 1:+70108975 MsaT004485.1:CDS
CTAGAAATGTGAGCCATGAA+AGG 0.580946 1:+70109156 MsaT004485.1:CDS
ACTGACTACTGAAGAAGTGA+AGG 0.595360 1:+70109301 MsaT004485.1:CDS
TCCATTTGATTCCCAGACAG+AGG 0.627731 1:+70109064 MsaT004485.1:CDS
TGAGAATCGTGAGCAAGAGT+GGG 0.628956 1:+70109232 MsaT004485.1:CDS
GAGGTTTCAAAGTTTAGCTG+TGG 0.630642 1:+70109124 MsaT004485.1:CDS
GAGGTGTGGCCACATCCAGA+AGG 0.649175 1:+70109083 MsaT004485.1:CDS
GAGCCAATAAGATAATTGAG+AGG 0.656204 1:+70109105 MsaT004485.1:CDS
TTGATTCCCAGACAGAGGTG+TGG 0.667220 1:+70109069 MsaT004485.1:CDS
GCAGACGATTGTCAAAGCAA+GGG 0.675137 1:+70109187 MsaT004485.1:CDS
TGCTGAGAAGAAGTTGAAGG+AGG 0.675328 1:+70109337 MsaT004485.1:CDS
TTATTCCCGATGATGCAGCA+AGG 0.681845 1:+70108946 MsaT004485.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 70108918 70109391 70108918 ID=MsaG004485
Chr1 mRNA 70108918 70109391 70108918 ID=MsaT004485.1;Parent=MsaG004485
Chr1 exon 70108918 70109391 70108918 ID=MsaT004485.1.exon1;Parent=MsaT004485.1
Chr1 CDS 70108918 70109391 70108918 ID=cds.MsaT004485.1;Parent=MsaT004485.1
Gene Sequence

>MsaT004485.1

ATGGCGAGAAGAAAGGTGAAACTCGCCTTTATTCCCGATGATGCAGCAAGGAAGGCAAGCTACAATAAAAGAAAGAAGGGTCTCATAAAGAAGGTAAGTGAACTCACCATTCTTTGTGATGTTCCAGCTTGTGCTATAATTTTCAGTCCATTTGATTCCCAGACAGAGGTGTGGCCACATCCAGAAGGAGCCAATAAGATAATTGAGAGGTTTCAAAGTTTAGCTGTGGTAGATGAAACTAGAAATGTGAGCCATGAAAGGTTTATCATGCAGACGATTGTCAAAGCAAGGGACAAACTAAGGAAGTTGAAATATGAGAATCGTGAGCAAGAGTGGGATCTTTTTTTGCTCAGGAACTTGAAAAACAACAATGTGCTTGATGAACTGACTACTGAAGAAGTGAAGGATCTTGGTAAGCGTGCTGAGAAGAAGTTGAAGGAGGTTAATGAGAAGATTGATACACTTGATGGATGA
Protein sequence

>MsaT004485.1

MARRKVKLAFIPDDAARKASYNKRKKGLIKKVSELTILCDVPACAIIFSPFDSQTEVWPHPEGANKIIERFQSLAVVDETRNVSHERFIMQTIVKARDKLRKLKYENREQEWDLFLLRNLKNNNVLDELTTEEVKDLGKRAEKKLKEVNEKIDTLDG*