AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004498

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004498 MtrunA17_Chr1g0197461 96.154 156 6 0 1 156 1 156 2.49e-108 305
MsaG004498 MtrunA17_Chr5g0420351 66.013 153 52 0 2 154 3 155 6.36e-70 208
MsaG004498 MtrunA17_Chr1g0197261 61.039 154 60 0 2 155 3 156 4.19e-61 186
MsaG004498 MtrunA17_Chr1g0197321 60.645 155 60 1 2 155 3 157 4.99e-61 185
MsaG004498 MtrunA17_Chr1g0188681 57.143 154 66 0 1 154 2 155 5.82e-59 180
MsaG004498 MtrunA17_Chr1g0193311 63.399 153 56 0 2 154 3 155 6.26e-58 177
MsaG004498 MtrunA17_Chr1g0188661 56.863 153 66 0 2 154 3 155 2.52e-52 163
MsaG004498 MtrunA17_Chr7g0218151 56.209 153 67 0 2 154 3 155 8.48e-52 162
MsaG004498 MtrunA17_Chr4g0012761 56.863 153 66 0 2 154 3 155 3.28e-51 160
MsaG004498 MtrunA17_Chr1g0188651 57.792 154 63 1 1 154 2 153 1.83e-49 156
MsaG004498 MtrunA17_Chr3g0107351 43.949 157 87 1 1 156 1 157 2.92e-40 133
MsaG004498 MtrunA17_Chr3g0107331 43.312 157 88 1 1 156 1 157 1.38e-38 129
MsaG004498 MtrunA17_Chr3g0107361 42.038 157 90 1 1 156 1 157 1.46e-37 126
MsaG004498 MtrunA17_Chr3g0107341 42.038 157 90 1 1 156 1 157 7.14e-37 124
MsaG004498 MtrunA17_Chr4g0014321 39.456 147 87 2 2 146 3 149 4.47e-34 119
MsaG004498 MtrunA17_Chr4g0013041 40.690 145 82 3 4 146 5 147 9.08e-34 117
MsaG004498 MtrunA17_Chr3g0107381 37.500 152 94 1 2 152 3 154 5.80e-33 116
MsaG004498 MtrunA17_Chr8g0351751 37.975 158 94 2 2 155 3 160 6.92e-33 116
MsaG004498 MtrunA17_Chr3g0110131 41.060 151 88 1 2 151 3 153 6.06e-30 108
MsaG004498 MtrunA17_Chr4g0014031 40.260 154 90 2 2 153 3 156 3.34e-29 106
MsaG004498 MtrunA17_Chr2g0283941 37.419 155 95 2 3 155 4 158 9.88e-27 100
MsaG004498 MtrunA17_Chr5g0432691 32.468 154 103 1 3 155 4 157 2.13e-26 97.8
MsaG004498 MtrunA17_Chr2g0296831 40.336 119 70 1 2 119 3 121 6.90e-26 95.1
MsaG004498 MtrunA17_Chr4g0014121 40.541 148 86 2 1 146 30 177 1.56e-23 90.9
MsaG004498 MtrunA17_Chr3g0107461 31.613 155 104 2 2 154 3 157 7.28e-23 88.6
MsaG004498 MtrunA17_Chr4g0014131 39.610 154 91 2 2 153 3 156 4.67e-22 88.2
MsaG004498 MtrunA17_Chr2g0296861 34.641 153 99 1 5 156 6 158 1.02e-21 85.5
MsaG004498 MtrunA17_Chr4g0031421 38.760 129 71 4 28 151 1 126 2.86e-19 79.0
MsaG004498 MtrunA17_Chr3g0105791 33.884 121 78 2 28 146 1 121 9.03e-17 72.4
MsaG004498 MtrunA17_Chr3g0106501 36.364 132 78 4 28 155 1 130 1.87e-16 72.0
MsaG004498 MtrunA17_Chr3g0106421 34.091 132 83 3 28 156 1 131 2.07e-15 70.5
MsaG004498 MtrunA17_Chr1g0162261 42.056 107 55 3 55 154 8 114 5.35e-15 67.0
MsaG004498 MtrunA17_Chr4g0008701 25.517 145 107 1 2 145 6 150 8.01e-15 70.5
MsaG004498 MtrunA17_Chr1g0161931 40.206 97 57 1 56 151 21 117 1.40e-14 66.2
MsaG004498 MtrunA17_Chr7g0267601 25.175 143 100 1 2 144 6 141 5.46e-14 68.2
MsaG004498 MtrunA17_Chr3g0128721 24.516 155 116 1 2 155 6 160 7.86e-14 67.8
MsaG004498 MtrunA17_Chr1g0162181 42.308 104 53 3 55 151 8 111 3.51e-13 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004498 AT1G65300.1 44.828 145 79 1 1 144 1 145 2.70e-38 132
MsaG004498 AT1G65330.1 45.517 145 78 1 1 144 1 145 1.72e-37 129
MsaG004498 AT3G05860.2 40.411 146 86 1 2 146 3 148 8.07e-32 113
MsaG004498 AT3G05860.3 40.411 146 86 1 2 146 3 148 2.39e-31 113
MsaG004498 AT3G05860.1 40.411 146 86 1 2 146 3 148 3.02e-31 113
MsaG004498 AT5G26630.1 41.722 151 87 1 2 151 3 153 4.12e-31 111
MsaG004498 AT5G26650.1 40.132 152 90 1 4 154 3 154 1.33e-30 113
MsaG004498 AT5G48670.1 41.722 151 87 1 2 151 3 153 5.33e-30 111
MsaG004498 AT5G27960.1 38.816 152 92 1 4 154 3 154 1.57e-28 107
MsaG004498 AT1G31630.1 32.895 152 100 2 1 151 1 151 1.07e-26 102
MsaG004498 AT1G31640.1 34.014 147 95 2 1 146 1 146 4.04e-23 94.4
MsaG004498 AT1G22590.2 32.903 155 102 1 2 156 3 155 4.47e-21 84.3
MsaG004498 AT5G26580.1 33.784 148 85 1 4 151 5 139 4.22e-19 82.4
MsaG004498 AT5G27810.1 36.559 93 58 1 28 119 1 93 4.85e-19 77.8
MsaG004498 AT2G28700.1 30.137 146 99 3 2 145 3 147 6.45e-18 79.0
MsaG004498 AT5G06500.1 30.612 147 94 4 2 144 3 145 9.50e-13 63.9
MsaG004498 AT2G40210.1 32.857 140 90 3 2 139 3 140 5.09e-11 60.1

Find 25 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCTCTTGGTTCAAATTCT+TGG 0.209087 1:-70226396 None:intergenic
ACCTCTACTTGAGAATTAAA+AGG 0.282594 1:-70226305 None:intergenic
ACCCTTCTTTCGTTTGTTGT+AGG 0.315510 1:-70226216 None:intergenic
TGCCGCACCATTGGAGATAA+AGG 0.336190 1:-70226183 None:intergenic
TGCTGAAGAACTGAAAGATT+TGG 0.355282 1:+70226547 MsaT004498.1:CDS
TGCCTTCCTTGCCGCACCAT+TGG 0.403210 1:-70226192 None:intergenic
TTAATTCTCAAGTAGAGGTC+TGG 0.432525 1:+70226309 MsaT004498.1:CDS
TATTGAGAAGAAGCTGAAAG+AGG 0.440765 1:+70226577 MsaT004498.1:CDS
AAAGAACTCACAACTCTTTG+TGG 0.452870 1:+70226254 MsaT004498.1:CDS
AACCTACAACAAACGAAAGA+AGG 0.478774 1:+70226214 MsaT004498.1:CDS
TTTGCATGATTAAACCCTCT+TGG 0.480889 1:-70226409 None:intergenic
CTCCAATGGTGCGGCAAGGA+AGG 0.499386 1:+70226190 MsaT004498.1:CDS
AAACTTGCCTTTATCTCCAA+TGG 0.509814 1:+70226176 MsaT004498.1:CDS
ACCAAGAATTTGAACCAAGA+GGG 0.512184 1:+70226395 MsaT004498.1:CDS
CGACGTAGCCATGAGACAAA+AGG 0.530990 1:+70226151 None:intergenic
TAGAGACCGGCTAAGAAAGT+TGG 0.534113 1:+70226445 MsaT004498.1:CDS
AAAGAAGGGTATCATAAAGA+AGG 0.534887 1:+70226229 MsaT004498.1:CDS
AGAGGTCTGGCCAGATCGAG+AGG 0.578371 1:+70226322 MsaT004498.1:CDS
TGCCTTTATCTCCAATGGTG+CGG 0.586809 1:+70226181 MsaT004498.1:CDS
ATATTGCTAAAGCTAGAGAC+CGG 0.607356 1:+70226432 MsaT004498.1:CDS
TTATCTCCAATGGTGCGGCA+AGG 0.627345 1:+70226186 MsaT004498.1:CDS
CTTTCAGTTCTTCAGCAGTG+AGG 0.630045 1:-70226541 None:intergenic
AACCAAGAATTTGAACCAAG+AGG 0.635779 1:+70226394 MsaT004498.1:CDS
ACCTACAACAAACGAAAGAA+GGG 0.643388 1:+70226215 MsaT004498.1:CDS
GAGGTCTGGCCAGATCGAGA+GGG 0.647218 1:+70226323 MsaT004498.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 70226161 70226631 70226161 ID=MsaG004498
Chr1 mRNA 70226161 70226631 70226161 ID=MsaT004498.1;Parent=MsaG004498
Chr1 exon 70226161 70226631 70226161 ID=MsaT004498.1.exon1;Parent=MsaT004498.1
Chr1 CDS 70226161 70226631 70226161 ID=cds.MsaT004498.1;Parent=MsaT004498.1
Gene Sequence

>MsaT004498.1

ATGAGACAAAAGGTGAAACTTGCCTTTATCTCCAATGGTGCGGCAAGGAAGGCAACCTACAACAAACGAAAGAAGGGTATCATAAAGAAGGTGAAAGAACTCACAACTCTTTGTGGTGTTTCTGCTTGTGCTATTATTTCAAATCCTTTTAATTCTCAAGTAGAGGTCTGGCCAGATCGAGAGGGAGCAAAAAAGGTGATTGAGAGGTATCAGAATTCATCTGTTAAAGATGAAACCAAGAATTTGAACCAAGAGGGTTTAATCATGCAAAATATTGCTAAAGCTAGAGACCGGCTAAGAAAGTTGGAAAACAAGAAACCTGAGAAAAAGATAGATCTGCTTATGTATGAGTGCATGCAAAACAAAAATTTGGTGGACAACCTCACTGCTGAAGAACTGAAAGATTTGGACGAGTTTATTGAGAAGAAGCTGAAAGAGGAAGATGATAAGATTAATGCAATTGAAACATGA
Protein sequence

>MsaT004498.1

MRQKVKLAFISNGAARKATYNKRKKGIIKKVKELTTLCGVSACAIISNPFNSQVEVWPDREGAKKVIERYQNSSVKDETKNLNQEGLIMQNIAKARDRLRKLENKKPEKKIDLLMYECMQNKNLVDNLTAEELKDLDEFIEKKLKEEDDKINAIET*