AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004627

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004627 MtrunA17_Chr1g0199101 93.404 470 17 3 1 457 71 539 0.0 893
MsaG004627 MtrunA17_Chr1g0163001 54.134 508 126 24 1 450 67 525 6.24e-141 414
MsaG004627 MtrunA17_Chr1g0210821 44.376 489 213 12 1 442 68 544 2.24e-107 329
MsaG004627 MtrunA17_Chr1g0162971 47.427 447 129 17 1 426 65 426 6.17e-106 322
MsaG004627 MtrunA17_Chr8g0342421 90.580 138 13 0 1 138 86 223 1.24e-94 295
MsaG004627 MtrunA17_Chr7g0263941 86.806 144 18 1 1 144 58 200 1.70e-94 291
MsaG004627 MtrunA17_Chr2g0330171 82.468 154 24 1 1 151 70 223 1.10e-93 291
MsaG004627 MtrunA17_Chr8g0373541 86.765 136 18 0 1 136 57 192 3.79e-92 288
MsaG004627 MtrunA17_Chr1g0181381 87.770 139 16 1 1 138 62 200 1.32e-91 286
MsaG004627 MtrunA17_Chr4g0040571 85.507 138 20 0 1 138 63 200 1.29e-90 284
MsaG004627 MtrunA17_Chr4g0029521 85.612 139 19 1 1 138 62 200 1.27e-89 281
MsaG004627 MtrunA17_Chr7g0268961 83.824 136 22 0 1 136 46 181 2.27e-89 280
MsaG004627 MtrunA17_Chr1g0191991 84.559 136 21 0 1 136 87 222 3.76e-89 281
MsaG004627 MtrunA17_Chr1g0151351 77.215 158 34 2 1 156 65 222 1.52e-88 277
MsaG004627 MtrunA17_Chr1g0185121 61.088 239 67 8 1 219 64 296 2.34e-88 276
MsaG004627 MtrunA17_Chr2g0326381 81.295 139 25 1 1 138 56 194 1.04e-83 263
MsaG004627 MtrunA17_Chr3g0141431 75.373 134 32 1 1 133 46 179 2.64e-77 246
MsaG004627 MtrunA17_Chr2g0324991 83.898 118 19 0 1 118 16 133 1.95e-73 228
MsaG004627 MtrunA17_Chr6g0479921 60.606 132 51 1 1 131 53 184 5.94e-58 197
MsaG004627 MtrunA17_Chr8g0375521 59.091 132 52 2 1 130 51 182 1.14e-55 190
MsaG004627 MtrunA17_Chr1g0200421 35.461 141 79 4 1 129 246 386 6.73e-22 98.2
MsaG004627 MtrunA17_Chr5g0422571 37.584 149 80 6 1 138 109 255 6.79e-21 92.8
MsaG004627 MtrunA17_Chr8g0352011 37.681 138 76 5 1 129 174 310 1.13e-19 90.1
MsaG004627 MtrunA17_Chr7g0258011 35.065 154 80 7 1 136 184 335 1.82e-19 89.4
MsaG004627 MtrunA17_Chr2g0320341 36.090 133 71 4 8 126 246 378 2.31e-19 89.7
MsaG004627 MtrunA17_Chr1g0198651 35.915 142 76 5 1 129 110 249 3.10e-19 87.8
MsaG004627 MtrunA17_Chr3g0123941 35.036 137 81 3 1 129 132 268 3.20e-19 88.6
MsaG004627 MtrunA17_Chr1g0173841 36.691 139 76 6 1 129 223 359 8.24e-19 88.2
MsaG004627 MtrunA17_Chr4g0053341 38.346 133 72 5 6 129 87 218 3.07e-18 83.6
MsaG004627 MtrunA17_Chr2g0328921 35.252 139 78 6 1 129 146 282 6.54e-18 84.3
MsaG004627 MtrunA17_Chr2g0321291 37.037 135 73 5 5 129 145 277 2.29e-17 82.8
MsaG004627 MtrunA17_Chr4g0026521 36.296 135 74 6 5 129 130 262 2.58e-17 82.4
MsaG004627 MtrunA17_Chr8g0382121 33.577 137 80 5 1 126 120 256 2.81e-16 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004627 AT2G02080.6 48.618 434 176 16 1 416 1 405 4.41e-107 325
MsaG004627 AT2G02080.2 48.618 434 176 16 1 416 1 405 4.41e-107 325
MsaG004627 AT2G02080.1 48.387 434 177 16 1 416 78 482 3.01e-106 325
MsaG004627 AT2G02080.4 48.387 434 177 16 1 416 78 482 3.01e-106 325
MsaG004627 AT2G02080.5 48.387 434 177 16 1 416 78 482 3.01e-106 325
MsaG004627 AT2G02080.3 48.387 434 177 16 1 416 78 482 3.01e-106 325
MsaG004627 AT2G02070.2 84.568 162 19 2 1 162 76 231 2.20e-97 305
MsaG004627 AT2G02070.1 84.568 162 19 2 1 162 76 231 2.20e-97 305
MsaG004627 AT1G14580.1 90.141 142 13 1 1 142 77 217 2.97e-95 295
MsaG004627 AT1G14580.2 90.141 142 13 1 1 142 77 217 2.97e-95 295
MsaG004627 AT1G14580.3 90.141 142 13 1 1 142 91 231 3.71e-95 295
MsaG004627 AT3G50700.1 84.397 141 22 0 1 141 58 198 3.57e-92 287
MsaG004627 AT1G55110.3 85.616 146 20 1 1 145 87 232 6.53e-92 286
MsaG004627 AT1G55110.1 85.616 146 20 1 1 145 87 232 6.53e-92 286
MsaG004627 AT1G55110.2 85.616 146 20 1 1 145 87 232 6.53e-92 286
MsaG004627 AT5G66730.1 85.106 141 21 0 1 141 56 196 2.33e-91 286
MsaG004627 AT3G45260.2 68.000 200 55 2 1 191 63 262 5.83e-91 283
MsaG004627 AT3G45260.1 68.000 200 55 2 1 191 63 262 5.83e-91 283
MsaG004627 AT1G03840.2 83.333 138 23 0 1 138 63 200 1.28e-88 279
MsaG004627 AT1G03840.1 83.333 138 23 0 1 138 65 202 1.59e-88 279
MsaG004627 AT5G03150.1 80.128 156 26 2 1 151 77 232 9.55e-88 277
MsaG004627 AT3G13810.3 86.331 139 18 1 1 138 81 219 1.06e-87 276
MsaG004627 AT3G13810.6 86.331 139 18 1 1 138 75 213 1.10e-87 276
MsaG004627 AT3G13810.5 86.331 139 18 1 1 138 89 227 1.27e-87 277
MsaG004627 AT3G13810.4 86.331 139 18 1 1 138 94 232 1.53e-87 276
MsaG004627 AT3G13810.1 86.331 139 18 1 1 138 94 232 1.53e-87 276
MsaG004627 AT3G13810.2 86.331 139 18 1 1 138 95 233 1.62e-87 276
MsaG004627 AT5G44160.2 84.058 138 22 0 1 138 22 159 2.16e-87 273
MsaG004627 AT5G44160.1 84.058 138 22 0 1 138 61 198 3.64e-87 274
MsaG004627 AT5G60470.1 76.667 150 32 2 1 147 59 208 7.64e-86 270
MsaG004627 AT4G02670.1 76.623 154 26 2 1 144 77 230 2.16e-85 268
MsaG004627 AT4G02670.2 76.623 154 26 2 1 144 79 232 2.27e-85 268
MsaG004627 AT5G60470.2 76.667 150 32 2 1 147 68 217 2.50e-85 269
MsaG004627 AT5G60470.3 76.667 150 32 2 1 147 64 213 3.05e-85 269
MsaG004627 AT1G25250.1 44.248 226 98 6 1 222 34 235 2.72e-59 199
MsaG004627 AT1G25250.3 44.248 226 98 6 1 222 57 258 3.69e-59 199
MsaG004627 AT1G68130.1 48.039 204 87 5 1 202 65 251 5.56e-59 199
MsaG004627 AT2G01940.1 62.687 134 48 2 1 132 68 201 1.43e-57 196
MsaG004627 AT2G01940.3 61.481 135 49 3 1 132 68 202 5.70e-54 187
MsaG004627 AT1G25250.2 42.647 204 89 6 23 222 1 180 6.26e-46 162
MsaG004627 AT1G68130.2 46.703 182 78 5 23 202 1 165 6.78e-46 162
MsaG004627 AT2G01940.2 62.500 112 40 2 23 132 1 112 3.32e-44 159
MsaG004627 AT1G34370.3 34.568 162 92 4 1 148 29 190 5.47e-21 93.2
MsaG004627 AT1G34370.2 34.568 162 92 4 1 148 239 400 7.80e-21 95.5
MsaG004627 AT1G34370.1 34.568 162 92 4 1 148 239 400 7.80e-21 95.5
MsaG004627 AT5G22890.1 32.168 143 83 3 1 129 212 354 1.51e-19 90.5
MsaG004627 AT1G13290.1 36.000 150 82 6 1 138 96 243 3.45e-19 88.2
MsaG004627 AT3G20880.1 34.783 138 80 5 1 129 251 387 1.30e-18 88.2
MsaG004627 AT1G51220.1 31.677 161 96 6 1 152 173 328 1.53e-18 87.0
MsaG004627 AT3G57670.1 35.971 139 77 6 1 129 212 348 3.26e-18 86.7
MsaG004627 AT1G08290.1 37.500 136 72 6 5 129 179 312 1.35e-17 84.3
MsaG004627 AT1G34790.1 38.806 134 70 6 6 129 145 276 2.06e-17 83.2

Find 81 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACTTGATGATCACTTAA+AGG 0.176750 1:-71918192 None:intergenic
ATTGCTTTCATTGCCAAATA+TGG 0.188179 1:-71918495 None:intergenic
TTTGCAATTGATGGGATATT+TGG 0.199708 1:-71917792 None:intergenic
GCTTCAAAAGCTTCTCAAAT+TGG 0.212468 1:+71918278 MsaT004627.1:intron
GCACATTCAACTCAACATTT+TGG 0.223217 1:+71918674 MsaT004627.1:CDS
AACAACATCAATGTCACTTT+TGG 0.258147 1:+71918383 MsaT004627.1:CDS
AATGATGAAGAGTGATTTAA+AGG 0.258726 1:-71917913 None:intergenic
GGAAACACATCATCCATATT+TGG 0.261465 1:+71918482 MsaT004627.1:CDS
TGCTAGTGGATGATGTAATT+TGG 0.279783 1:-71917825 None:intergenic
TGGAAATAGAAGTTCAAGTA+TGG 0.282481 1:+71918403 MsaT004627.1:CDS
CTTTCTCCTTGGCTCTTTGT+TGG 0.310223 1:-71915818 None:intergenic
TTAAATCACTCTTCATCATT+TGG 0.318218 1:+71917916 MsaT004627.1:CDS
TTAAGACACCCTAACCCTTT+TGG 0.323474 1:+71917724 MsaT004627.1:CDS
CACGACCCTTCAAGAGCTTT+AGG 0.336517 1:+71915874 MsaT004627.1:CDS
AGCAATCTTAATATGAGATT+TGG 0.345761 1:+71918512 MsaT004627.1:CDS
GATGACAACAACGATCTTCA+TGG 0.372227 1:+71918437 MsaT004627.1:CDS
AACCATAAGGTTGAAAAGAT+TGG 0.377417 1:-71918106 None:intergenic
CAACTTGATGATCACTTAAA+GGG 0.378987 1:-71918191 None:intergenic
ACAAACCATCACATTAGTTT+AGG 0.387648 1:+71917769 MsaT004627.1:CDS
ATGATCATAAATCAGCTGAT+AGG 0.388164 1:+71918339 MsaT004627.1:CDS
ATTACTACCTTTGTTATTGT+TGG 0.391654 1:-71918315 None:intergenic
TGAGTACTCCCAAAAGGGTT+AGG 0.396876 1:-71917733 None:intergenic
TTTGTATTGCCTTGAAGATC+AGG 0.407490 1:-71918063 None:intergenic
TTGATAAACTCTATTGCAAA+TGG 0.417216 1:+71918461 MsaT004627.1:CDS
GTGACAGTTTCATCACTCAT+AGG 0.424200 1:+71917668 MsaT004627.1:CDS
TTTGACCATGTTGCATTGAA+TGG 0.424923 1:-71918221 None:intergenic
AATATGCTGTTCAATCTGAT+TGG 0.427517 1:+71915974 MsaT004627.1:CDS
ATGCATCTTCCTGATCTTCA+AGG 0.433107 1:+71918054 MsaT004627.1:CDS
GCTCAAATATGTTGCAATGA+AGG 0.444416 1:+71918699 MsaT004627.1:CDS
TTGAGATTGTTGTTCTTCAA+AGG 0.461712 1:-71917995 None:intergenic
CACAAATAAACCTTTCTCCT+TGG 0.464750 1:-71915829 None:intergenic
TTGTTCCTTGGTTACTACTC+AGG 0.465952 1:-71918146 None:intergenic
TATGTGAGGTTTGCAACAAA+GGG 0.467995 1:+71915725 MsaT004627.1:CDS
TGATGGACGCATGTGGGCTC+AGG 0.479979 1:-71915853 None:intergenic
GAAGAGGACACAATCTTCCT+TGG 0.488329 1:+71915779 MsaT004627.1:CDS
AATTGATGAGTACTCCCAAA+AGG 0.490054 1:-71917739 None:intergenic
CCTTTAAGTGATCATCAAGT+TGG 0.496965 1:+71918192 MsaT004627.1:CDS
CAACAAAGAGCCAAGGAGAA+AGG 0.497525 1:+71915819 MsaT004627.1:CDS
TAAACTAATGTGATGGTTTG+TGG 0.499948 1:-71917767 None:intergenic
TGATCATAAATCAGCTGATA+GGG 0.500020 1:+71918340 MsaT004627.1:CDS
ATTAAGTGTTGTTCAAACTT+TGG 0.503007 1:-71917880 None:intergenic
TGAATTGATCATGAACCATA+AGG 0.503794 1:-71918119 None:intergenic
AATCTTAATATGAGATTTGG+TGG 0.509729 1:+71918515 MsaT004627.1:CDS
CAAAATGTTGTTATTGCTAG+TGG 0.510794 1:-71917839 None:intergenic
GAGTACTCCCAAAAGGGTTA+GGG 0.513767 1:-71917732 None:intergenic
TTGAAGAATGGTGATGAAGA+TGG 0.514253 1:-71917949 None:intergenic
ATATGTGAGGTTTGCAACAA+AGG 0.517578 1:+71915724 MsaT004627.1:CDS
TAATGTAAAGTGTTGTTCCT+TGG 0.521540 1:-71918158 None:intergenic
TTTGCAACAAAGGGTTTCAA+AGG 0.521693 1:+71915734 MsaT004627.1:CDS
TTGCAACAAAGGGTTTCAAA+GGG 0.525797 1:+71915735 MsaT004627.1:CDS
ATTGATGAGTACTCCCAAAA+GGG 0.530292 1:-71917738 None:intergenic
ATTGTGGCACCCTCTTCTCT+AGG 0.536248 1:+71916040 MsaT004627.1:CDS
ATATCACTCATATTGAAGAA+TGG 0.537313 1:-71917961 None:intergenic
AGATCTCCTAAAGCTCTTGA+AGG 0.547138 1:-71915880 None:intergenic
ACTTTGGTGGTGTTGATCCA+AGG 0.547369 1:-71917864 None:intergenic
GTAAGCATGGTGAGAAGAAG+TGG 0.548240 1:+71915929 MsaT004627.1:CDS
AAAAGTCCAACAAAGAGCCA+AGG 0.555795 1:+71915812 MsaT004627.1:CDS
AATCTTCAACTTCATAGAAG+AGG 0.557392 1:+71915763 MsaT004627.1:CDS
AACAAATAGATTTATATGTG+AGG 0.560724 1:+71915711 MsaT004627.1:CDS
TTTGGCCTAAACTAATGTGA+TGG 0.560847 1:-71917774 None:intergenic
AAGCATTACTCTCGTAAGCA+TGG 0.570554 1:+71915916 MsaT004627.1:CDS
ATGTTTGTTACCTAGAGAAG+AGG 0.572256 1:-71916050 None:intergenic
AAGCTCTTGAAGGGTCGTGA+TGG 0.580721 1:-71915870 None:intergenic
TTCTTAAACAAACAACTACA+TGG 0.582618 1:+71918027 MsaT004627.1:CDS
TGACAGTTTCATCACTCATA+GGG 0.582640 1:+71917669 MsaT004627.1:CDS
TGTTTGTTACCTAGAGAAGA+GGG 0.585590 1:-71916049 None:intergenic
AGAGAATATAGATGTGATTG+TGG 0.589973 1:+71916024 MsaT004627.1:CDS
AGAGCTTTAGGAGATCTCAC+TGG 0.591272 1:+71915886 MsaT004627.1:CDS
AAGTGTTGTTCAAACTTTGG+TGG 0.596159 1:-71917877 None:intergenic
ATGGTCAGAAACATGAGTGG+TGG 0.622609 1:+71918578 MsaT004627.1:CDS
TTTGTCCATTCAATGCAACA+TGG 0.623181 1:+71918216 MsaT004627.1:CDS
AAAGCACATTCTAAGACTTG+TGG 0.628477 1:+71915997 MsaT004627.1:CDS
AGGGTCGTGATGGACGCATG+TGG 0.629562 1:-71915860 None:intergenic
GATCTCCTAAAGCTCTTGAA+GGG 0.629745 1:-71915879 None:intergenic
GGGTCGTGATGGACGCATGT+GGG 0.641981 1:-71915859 None:intergenic
TGACATGATGAATACTAGCA+TGG 0.643758 1:+71918625 MsaT004627.1:CDS
AATTCTACCAACAATAACAA+AGG 0.649478 1:+71918308 MsaT004627.1:CDS
GATGAAGAGTGATTTAAAGG+TGG 0.652236 1:-71917910 None:intergenic
AACAACCTGAGTAGTAACCA+AGG 0.667707 1:+71918141 MsaT004627.1:CDS
GAAGAACAACAATCTCAACA+AGG 0.728667 1:+71918000 MsaT004627.1:CDS
GGAATGGTCAGAAACATGAG+TGG 0.767875 1:+71918575 MsaT004627.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 71915706 71918718 71915706 ID=MsaG004627
Chr1 mRNA 71915706 71918718 71915706 ID=MsaT004627.1;Parent=MsaG004627
Chr1 exon 71915706 71916061 71915706 ID=MsaT004627.1.exon1;Parent=MsaT004627.1
Chr1 CDS 71915706 71916061 71915706 ID=cds.MsaT004627.1;Parent=MsaT004627.1
Chr1 exon 71917666 71918252 71917666 ID=MsaT004627.1.exon2;Parent=MsaT004627.1
Chr1 CDS 71917666 71918252 71917666 ID=cds.MsaT004627.1;Parent=MsaT004627.1
Chr1 exon 71918288 71918718 71918288 ID=MsaT004627.1.exon3;Parent=MsaT004627.1
Chr1 CDS 71918288 71918718 71918288 ID=cds.MsaT004627.1;Parent=MsaT004627.1
Gene Sequence

>MsaT004627.1

ATGGCAACAAATAGATTTATATGTGAGGTTTGCAACAAAGGGTTTCAAAGGGAGCAAAATCTTCAACTTCATAGAAGAGGACACAATCTTCCTTGGAAGCTAAAGCAAAAGTCCAACAAAGAGCCAAGGAGAAAGGTTTATTTGTGTCCTGAGCCCACATGCGTCCATCACGACCCTTCAAGAGCTTTAGGAGATCTCACTGGAATTAAAAAGCATTACTCTCGTAAGCATGGTGAGAAGAAGTGGAAATGTGAAAAGTGCTCAAAGAAATATGCTGTTCAATCTGATTGGAAAGCACATTCTAAGACTTGTGGTACTAGAGAATATAGATGTGATTGTGGCACCCTCTTCTCTAGGCGTGACAGTTTCATCACTCATAGGGCATTTTGTGATGCACTAGCACAAGAGAGTGTATTAAGACACCCTAACCCTTTTGGGAGTACTCATCAATTGTATGCCACAAACCATCACATTAGTTTAGGCCAAATATCCCATCAATTGCAAAATCATCAGAACCAAATTACATCATCCACTAGCAATAACAACATTTTGCGCCTTGGATCAACACCACCAAAGTTTGAACAACACTTAATATCATCACCACCTTTAAATCACTCTTCATCATTTGGACACTCAATTCCATCTTCATCACCATTCTTCAATATGAGTGATATTTCAAATCAATCCTTTGAAGAACAACAATCTCAACAAGGACAATTCTTAAACAAACAACTACATGGTCTAATGCATCTTCCTGATCTTCAAGGCAATACAAATACAAACAATTCAAATTCTTCCAATCTTTTCAACCTTATGGTTCATGATCAATTCAACAACCTGAGTAGTAACCAAGGAACAACACTTTACATTAACAACAATAACCCTTTAAGTGATCATCAAGTTGGTTTTGTCCATTCAATGCAACATGGTCAAAACATGTCTACTTCTCAAATTGGTTCAACTAATTCTACCAACAATAACAAAGGTAGTAATAATGATCATAAATCAGCTGATAGGGACTTTGTTGTGTCTGATCACAACAACATCAATGTCACTTTTGGAAATAGAAGTTCAAGTATGGAAAATGATCATGATGACAACAACGATCTTCATGGATTGATAAACTCTATTGCAAATGGAAACACATCATCCATATTTGGCAATGAAAGCAATCTTAATATGAGATTTGGTGGATCTGATAAATTAACTTTAGATTTTCTTGGTGTTGGAGGAATGGTCAGAAACATGAGTGGTGGATTTTCACAAAGTGAACAACAACGTGACATGATGAATACTAGCATGGCTTCTTTGAATCATGACCTAAAATCAGCACATTCAACTCAACATTTTGGAAGCTCAAATATGTTGCAATGA
Protein sequence

>MsaT004627.1

MATNRFICEVCNKGFQREQNLQLHRRGHNLPWKLKQKSNKEPRRKVYLCPEPTCVHHDPSRALGDLTGIKKHYSRKHGEKKWKCEKCSKKYAVQSDWKAHSKTCGTREYRCDCGTLFSRRDSFITHRAFCDALAQESVLRHPNPFGSTHQLYATNHHISLGQISHQLQNHQNQITSSTSNNNILRLGSTPPKFEQHLISSPPLNHSSSFGHSIPSSSPFFNMSDISNQSFEEQQSQQGQFLNKQLHGLMHLPDLQGNTNTNNSNSSNLFNLMVHDQFNNLSSNQGTTLYINNNNPLSDHQVGFVHSMQHGQNMSTSQIGSTNSTNNNKGSNNDHKSADRDFVVSDHNNINVTFGNRSSSMENDHDDNNDLHGLINSIANGNTSSIFGNESNLNMRFGGSDKLTLDFLGVGGMVRNMSGGFSQSEQQRDMMNTSMASLNHDLKSAHSTQHFGSSNMLQ*