Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004680 | AFK34304.1 | 82.456 | 228 | 4 | 1 | 2 | 193 | 53 | 280 | 2.17e-130 | 377 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004680 | tr|I3S212|I3S212_MEDTR | 82.456 | 228 | 4 | 1 | 2 | 193 | 53 | 280 | 1.04e-130 | 377 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG004680 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004680 | MtrunA17_Chr1g0199701 | 82.456 | 228 | 4 | 1 | 2 | 193 | 53 | 280 | 2.04e-134 | 377 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004680 | AT2G39000.3 | 62.832 | 226 | 48 | 1 | 1 | 190 | 1 | 226 | 3.41e-102 | 294 |
| MsaG004680 | AT2G39000.1 | 62.667 | 225 | 48 | 1 | 2 | 190 | 58 | 282 | 3.71e-101 | 294 |
| MsaG004680 | AT2G39000.2 | 75.000 | 104 | 26 | 0 | 2 | 105 | 58 | 161 | 1.23e-52 | 168 |
| MsaG004680 | AT2G39000.4 | 75.000 | 104 | 26 | 0 | 2 | 105 | 58 | 161 | 1.88e-52 | 167 |
Find 43 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGATCAAACTTTCTTATT+TGG | 0.151315 | 1:-72611290 | MsaT004680.1:CDS |
| ACCTGTCTGGTTGCTGTTAT+TGG | 0.195810 | 1:-72611320 | MsaT004680.1:CDS |
| TTTCAAATCTATAAAGGTTC+CGG | 0.297267 | 1:-72609901 | MsaT004680.1:CDS |
| GCAAGGTTTCAAATCTATAA+AGG | 0.323365 | 1:-72609907 | MsaT004680.1:CDS |
| TCTAATGGAAATGAATATTC+AGG | 0.338599 | 1:+72611413 | None:intergenic |
| AGGCAAGAATAGAGGATTGT+TGG | 0.356437 | 1:-72611469 | MsaT004680.1:CDS |
| ATCCTCAACACAAAATCTAA+TGG | 0.371362 | 1:+72611398 | None:intergenic |
| GGCACGACATCCCCAACTTC+TGG | 0.376576 | 1:+72609983 | None:intergenic |
| GGAAGTGAAGACTTCAAGAT+TGG | 0.387372 | 1:-72611269 | MsaT004680.1:CDS |
| GCCTCTCTAACAGTGATCTC+AGG | 0.394323 | 1:+72611488 | None:intergenic |
| GGCAAGAATAGAGGATTGTT+GGG | 0.399207 | 1:-72611468 | MsaT004680.1:CDS |
| TTCAAATCTATAAAGGTTCC+GGG | 0.414198 | 1:-72609900 | MsaT004680.1:CDS |
| TGTGTTGAGGATGGATAGAT+TGG | 0.415569 | 1:-72611387 | MsaT004680.1:CDS |
| AGAATCACAAGCCAGAAGTT+GGG | 0.428773 | 1:-72609994 | MsaT004680.1:CDS |
| AGGTTCCGGGAGGAGCTAAC+TGG | 0.431391 | 1:-72609887 | MsaT004680.1:CDS |
| AGCTAAACAATTGGTGGCTA+AGG | 0.435007 | 1:-72610018 | MsaT004680.1:CDS |
| CAGAATCACAAGCCAGAAGT+TGG | 0.456663 | 1:-72609995 | MsaT004680.1:CDS |
| GCAGGATTCTCTTTACCAAA+TGG | 0.460775 | 1:-72611353 | MsaT004680.1:CDS |
| GAAAGGAATAGCTAAACAAT+TGG | 0.467703 | 1:-72610027 | MsaT004680.1:CDS |
| TAGATTTGAAACCTTGCCCT+TGG | 0.481800 | 1:+72609913 | None:intergenic |
| ATGAAGTTGAACTTCACATC+TGG | 0.483678 | 1:+72609846 | None:intergenic |
| GAAGTGAAGACTTCAAGATT+GGG | 0.485242 | 1:-72611268 | MsaT004680.1:intron |
| TGGTATAACTTTGTTGCCGC+AGG | 0.491255 | 1:+72609933 | None:intergenic |
| AGATTGGTGGCTATGTTAGC+AGG | 0.496959 | 1:-72611371 | MsaT004680.1:CDS |
| GAACAAATCAACAACTTGAC+TGG | 0.498714 | 1:+72611523 | None:intergenic |
| TGTTAGAGAGGCAAGAATAG+AGG | 0.524179 | 1:-72611477 | MsaT004680.1:CDS |
| GCCAATAACAGCAACCAGAC+AGG | 0.535502 | 1:+72611319 | None:intergenic |
| AATAGAGGATTGTTGGGAAG+TGG | 0.538776 | 1:-72611462 | MsaT004680.1:CDS |
| GAATCACAAGCCAGAAGTTG+GGG | 0.540089 | 1:-72609993 | MsaT004680.1:CDS |
| AGGAATAGCTAAACAATTGG+TGG | 0.557067 | 1:-72610024 | MsaT004680.1:CDS |
| CCTCTCTAACAGTGATCTCA+GGG | 0.558058 | 1:+72611489 | None:intergenic |
| TGGTTGCAAAAGAACCTGTC+TGG | 0.566369 | 1:-72611333 | MsaT004680.1:CDS |
| CCCTGAGATCACTGTTAGAG+AGG | 0.573199 | 1:-72611489 | MsaT004680.1:CDS |
| GTTGAGGATGGATAGATTGG+TGG | 0.579094 | 1:-72611384 | MsaT004680.1:CDS |
| TGAGGCCAGTTAGCTCCTCC+CGG | 0.584487 | 1:+72609882 | None:intergenic |
| ACAAAGTTATACCAAGGGCA+AGG | 0.614543 | 1:-72609924 | MsaT004680.1:CDS |
| TAAATCGCAGTGTAATGCAA+TGG | 0.632285 | 1:+72609962 | None:intergenic |
| AATTGCATACATATCAAATG+TGG | 0.643430 | 1:-72610072 | MsaT004680.1:CDS |
| CGGCAACAAAGTTATACCAA+GGG | 0.644825 | 1:-72609929 | MsaT004680.1:CDS |
| GCGGCAACAAAGTTATACCA+AGG | 0.651915 | 1:-72609930 | MsaT004680.1:CDS |
| CTGCGATTTACGAAATCCTG+CGG | 0.698179 | 1:-72609949 | MsaT004680.1:CDS |
| AAATCTATAAAGGTTCCGGG+AGG | 0.724022 | 1:-72609897 | MsaT004680.1:CDS |
| CTCTCTAACAGTGATCTCAG+GGG | 0.746520 | 1:+72611490 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 72609812 | 72612234 | 72609812 | ID=MsaG004680 |
| Chr1 | mRNA | 72609812 | 72612234 | 72609812 | ID=MsaT004680.1;Parent=MsaG004680 |
| Chr1 | exon | 72609812 | 72610100 | 72609812 | ID=MsaT004680.1.exon3;Parent=MsaT004680.1 |
| Chr1 | CDS | 72609812 | 72610100 | 72609812 | ID=cds.MsaT004680.1;Parent=MsaT004680.1 |
| Chr1 | exon | 72611269 | 72611557 | 72611269 | ID=MsaT004680.1.exon2;Parent=MsaT004680.1 |
| Chr1 | CDS | 72611269 | 72611557 | 72611269 | ID=cds.MsaT004680.1;Parent=MsaT004680.1 |
| Chr1 | exon | 72612231 | 72612234 | 72612231 | ID=MsaT004680.1.exon1;Parent=MsaT004680.1 |
| Chr1 | CDS | 72612231 | 72612234 | 72612231 | ID=cds.MsaT004680.1;Parent=MsaT004680.1 |
| Gene Sequence |
| Protein sequence |
