AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004745


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004745 MtrunA17_Chr1g0200401 98.915 461 5 0 1 461 1 461 0.0 943
MsaG004745 MtrunA17_Chr7g0224271 33.898 413 221 14 70 459 290 673 7.44e-64 218
MsaG004745 MtrunA17_Chr2g0306641 31.553 412 229 15 68 461 113 489 1.32e-54 189
MsaG004745 MtrunA17_Chr8g0376381 31.910 398 221 16 74 459 163 522 1.73e-53 187
MsaG004745 MtrunA17_Chr7g0245601 32.718 379 222 10 85 459 443 792 2.82e-52 188
MsaG004745 MtrunA17_Chr4g0057291 32.426 404 232 13 74 461 286 664 9.71e-52 185
MsaG004745 MtrunA17_Chr3g0110971 31.463 410 222 15 74 459 213 587 3.73e-50 179
MsaG004745 MtrunA17_Chr5g0447691 30.995 442 250 15 38 459 138 544 1.24e-48 174
MsaG004745 MtrunA17_Chr4g0038761 31.783 387 219 11 85 459 89 442 2.52e-47 169
MsaG004745 MtrunA17_Chr3g0125811 28.967 397 238 12 79 459 202 570 1.89e-45 166
MsaG004745 MtrunA17_Chr1g0159461 29.723 397 235 12 79 459 224 592 2.60e-45 166
MsaG004745 MtrunA17_Chr5g0445331 29.064 406 234 14 77 459 158 532 7.70e-45 164
MsaG004745 MtrunA17_Chr4g0057891 29.782 413 234 15 72 460 141 521 9.07e-45 164
MsaG004745 MtrunA17_Chr4g0039661 32.161 398 225 13 76 459 189 555 1.83e-43 160
MsaG004745 MtrunA17_Chr3g0101141 28.894 398 241 13 77 459 172 542 3.00e-43 160
MsaG004745 MtrunA17_Chr1g0183641 30.576 399 232 15 70 458 113 476 1.87e-42 156
MsaG004745 MtrunA17_Chr4g0076091 30.348 402 233 15 75 458 170 542 2.47e-41 154
MsaG004745 MtrunA17_Chr5g0424131 30.602 415 241 12 70 461 111 501 4.17e-41 153
MsaG004745 MtrunA17_Chr2g0319091 28.817 465 257 17 22 459 162 579 5.81e-41 154
MsaG004745 MtrunA17_Chr4g0031741 30.025 403 237 13 72 458 276 649 9.89e-41 154
MsaG004745 MtrunA17_Chr3g0114841 31.221 426 220 16 70 458 110 499 3.32e-40 150
MsaG004745 MtrunA17_Chr2g0290241 30.079 379 211 14 103 460 81 426 2.43e-38 144
MsaG004745 MtrunA17_Chr8g0357601 29.298 413 229 17 74 459 176 552 1.10e-36 142
MsaG004745 MtrunA17_Chr4g0031711 29.750 400 238 14 77 458 358 732 2.63e-36 142
MsaG004745 MtrunA17_Chr4g0031701 29.188 394 236 13 77 452 251 619 3.63e-35 138
MsaG004745 MtrunA17_Chr4g0031731 29.640 361 222 12 77 419 251 597 2.28e-34 135
MsaG004745 MtrunA17_Chr7g0238891 27.204 397 244 14 80 459 372 740 8.22e-34 135
MsaG004745 MtrunA17_Chr2g0328341 29.146 398 231 15 80 458 272 637 8.41e-34 134
MsaG004745 MtrunA17_Chr2g0328411 26.087 414 259 13 61 458 271 653 1.02e-32 131
MsaG004745 MtrunA17_Chr2g0328391 26.895 409 244 14 73 458 292 668 1.62e-32 130
MsaG004745 MtrunA17_Chr2g0328381 27.665 394 242 13 80 458 275 640 2.27e-31 127
MsaG004745 MtrunA17_Chr2g0328431 25.758 396 249 12 77 458 289 653 9.73e-31 125
MsaG004745 MtrunA17_Chr4g0031721 28.173 394 242 15 80 458 316 683 4.51e-30 123
MsaG004745 MtrunA17_Chr2g0328401 26.685 356 232 9 77 418 287 627 1.87e-28 118
MsaG004745 MtrunA17_Chr2g0294461 25.542 415 265 12 65 459 195 585 5.60e-28 117
MsaG004745 MtrunA17_Chr8g0383981 30.479 397 224 18 77 458 136 495 2.61e-27 114
MsaG004745 MtrunA17_Chr5g0401131 27.451 408 239 15 79 459 113 490 5.46e-27 113
MsaG004745 MtrunA17_Chr2g0294471 25.234 428 247 15 65 459 189 576 5.90e-27 114
MsaG004745 MtrunA17_Chr3g0085681 23.661 448 256 13 71 459 16 436 6.50e-27 112
MsaG004745 MtrunA17_Chr7g0270031 25.845 414 255 14 67 461 176 556 2.32e-26 112
MsaG004745 MtrunA17_Chr2g0328361 26.209 393 224 15 80 458 274 614 4.77e-24 105
MsaG004745 MtrunA17_Chr2g0328371 32.157 255 135 13 160 396 12 246 1.13e-23 99.8
MsaG004745 MtrunA17_Chr7g0230731 26.718 393 245 15 77 459 369 728 1.77e-20 94.7
MsaG004745 MtrunA17_Chr7g0230771 26.368 402 235 16 77 459 372 731 2.57e-20 94.0
MsaG004745 MtrunA17_Chr6g0468561 26.895 409 227 15 81 459 296 662 9.57e-20 92.4
MsaG004745 MtrunA17_Chr3g0085361 23.333 360 193 12 156 459 18 350 9.97e-20 90.5
MsaG004745 MtrunA17_Chr4g0038851 28.889 225 150 4 71 293 39 255 3.28e-19 90.1
MsaG004745 MtrunA17_Chr4g0038851 31.325 166 96 4 295 460 323 470 7.18e-18 85.9
MsaG004745 MtrunA17_Chr7g0236851 26.263 396 240 13 81 458 295 656 3.82e-19 90.5
MsaG004745 MtrunA17_Chr5g0396491 27.473 273 160 9 71 336 47 288 5.33e-19 89.4
MsaG004745 MtrunA17_Chr5g0396491 29.070 172 104 4 289 460 326 479 1.23e-18 88.2
MsaG004745 MtrunA17_Chr5g0401421 23.333 450 252 14 76 458 22 445 1.70e-18 87.8
MsaG004745 MtrunA17_Chr2g0329591 24.590 366 240 11 111 459 105 451 2.65e-17 84.0
MsaG004745 MtrunA17_Chr8g0344101 23.227 409 256 14 79 459 222 600 1.92e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004745 AT3G54220.1 34.884 387 204 12 85 458 298 649 5.24e-55 194
MsaG004745 AT1G14920.1 32.767 412 225 16 63 459 155 529 1.54e-53 188
MsaG004745 AT2G04890.1 31.840 424 252 12 45 459 18 413 2.30e-52 182
MsaG004745 AT3G03450.1 32.587 402 222 16 74 459 177 545 2.46e-50 179
MsaG004745 AT5G41920.1 32.108 408 229 12 66 460 29 401 3.78e-50 176
MsaG004745 AT2G01570.1 30.941 404 223 16 73 459 217 581 2.50e-49 177
MsaG004745 AT1G21450.1 30.982 397 231 12 79 459 224 593 3.97e-49 177
MsaG004745 AT5G17490.1 32.039 412 224 18 64 459 145 516 1.77e-48 174
MsaG004745 AT1G66350.1 31.078 399 221 16 74 459 149 506 4.18e-47 170
MsaG004745 AT5G48150.1 30.380 395 232 13 81 459 123 490 1.34e-46 168
MsaG004745 AT5G48150.4 30.380 395 232 13 81 459 123 490 1.34e-46 168
MsaG004745 AT5G48150.2 30.380 395 232 13 81 459 123 490 1.34e-46 168
MsaG004745 AT5G48150.3 30.380 395 232 13 81 459 123 490 1.34e-46 168
MsaG004745 AT1G55580.1 31.075 428 221 16 80 459 44 445 3.23e-46 166
MsaG004745 AT1G63100.1 29.381 388 227 11 99 460 264 630 5.80e-46 169
MsaG004745 AT1G63100.2 29.381 388 227 11 99 460 289 655 1.12e-45 169
MsaG004745 AT5G66770.1 32.658 395 229 14 76 459 216 584 9.47e-45 165
MsaG004745 AT4G08250.1 31.127 408 238 14 70 460 97 478 2.34e-42 157
MsaG004745 AT1G50600.3 29.873 395 235 12 80 459 159 526 1.17e-41 155
MsaG004745 AT3G50650.1 33.155 374 214 12 98 459 193 542 1.38e-41 155
MsaG004745 AT1G50600.1 29.873 395 235 12 80 459 230 597 5.19e-41 155
MsaG004745 AT1G50600.2 29.873 395 235 12 80 459 234 601 6.86e-41 154
MsaG004745 AT4G17230.1 29.829 409 227 13 75 459 153 525 6.96e-37 142
MsaG004745 AT4G17230.2 29.829 409 227 13 75 459 153 525 6.96e-37 142
MsaG004745 AT1G50420.1 27.193 456 235 17 74 459 51 479 1.06e-31 127
MsaG004745 AT2G29060.2 27.456 397 250 13 80 461 284 657 1.35e-31 128
MsaG004745 AT2G29060.1 27.456 397 250 13 80 461 321 694 1.66e-31 128
MsaG004745 AT2G37650.2 25.505 396 252 13 80 460 335 702 2.86e-30 124
MsaG004745 AT2G37650.1 25.505 396 252 13 80 460 347 714 3.35e-30 124
MsaG004745 AT2G29065.1 25.980 408 241 16 80 458 252 627 2.49e-29 121
MsaG004745 AT1G07530.1 26.396 394 250 14 80 458 396 764 4.29e-28 118
MsaG004745 AT3G46600.2 26.196 397 251 13 79 460 3 372 1.05e-27 114
MsaG004745 AT5G59450.1 25.369 406 259 13 72 459 219 598 3.27e-27 115
MsaG004745 AT3G46600.1 26.329 395 251 13 79 459 211 579 3.50e-27 115
MsaG004745 AT1G07520.2 25.187 401 252 15 80 458 154 528 4.94e-27 114
MsaG004745 AT1G07520.4 25.187 401 252 15 80 458 154 528 4.94e-27 114
MsaG004745 AT3G46600.3 26.329 395 251 13 79 459 179 547 5.50e-27 114
MsaG004745 AT1G07520.1 25.187 401 252 15 80 458 318 692 6.44e-27 114
MsaG004745 AT1G07520.3 25.187 401 252 15 80 458 319 693 7.13e-27 114
MsaG004745 AT3G60630.1 29.909 331 172 15 152 459 329 622 8.46e-26 110
MsaG004745 AT4G37650.1 23.716 409 258 13 80 459 146 529 1.44e-21 97.8
MsaG004745 AT5G52510.1 25.679 405 242 15 81 459 269 640 1.84e-21 97.8
MsaG004745 AT2G45160.1 28.997 369 194 18 115 459 315 639 1.99e-21 97.8
MsaG004745 AT4G00150.1 27.446 368 208 17 105 459 233 554 1.02e-20 95.5
MsaG004745 AT4G36710.1 24.570 407 262 14 66 459 111 485 6.33e-20 92.8
MsaG004745 AT3G49950.1 23.058 412 252 13 79 458 29 407 6.65e-19 89.0

Find 57 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGGTGGTGGTGGTTTC+AGG 0.088549 1:+73340469 MsaT004745.1:CDS
GTGGAAACAGAATCTCTTTC+AGG 0.211105 1:+73339836 MsaT004745.1:CDS
TCTCTTTGGGATGGCAAAAT+AGG 0.239317 1:+73340520 MsaT004745.1:CDS
AGAATCTCTTTCAGGTTAAC+TGG 0.299270 1:+73339844 MsaT004745.1:CDS
TCTTTCAGGTTAACTGGTTT+CGG 0.304366 1:+73339850 MsaT004745.1:CDS
GTTAGTTTCTCAAAAGGTTT+TGG 0.310158 1:+73339913 MsaT004745.1:CDS
TGATGGAAGGGCTATCTCTT+TGG 0.326568 1:+73340506 MsaT004745.1:CDS
CACAGCAAATCAAGCTATCT+TGG 0.326969 1:+73339696 MsaT004745.1:CDS
GTGAGAAGTAAGTGAATTAA+TGG 0.332976 1:-73339418 None:intergenic
TCCATCAACATTTCATAGAA+TGG 0.366218 1:-73339595 None:intergenic
AGAAACTCTTCGTCGATTGT+TGG 0.372995 1:-73339622 None:intergenic
ATGGAGAATCATGGATTTGT+TGG 0.401767 1:+73340363 MsaT004745.1:CDS
ACCATTCTATGAAATGTTGA+TGG 0.404027 1:+73339594 MsaT004745.1:CDS
AGAAGGTGGTGGTGGTTTCA+GGG 0.404726 1:+73340470 MsaT004745.1:CDS
GATGGAAGGGCTATCTCTTT+GGG 0.413184 1:+73340507 MsaT004745.1:CDS
GAGTTTCAAGGTTTGTTAAG+AGG 0.425739 1:+73339949 MsaT004745.1:CDS
TTCTGTTGATGAAAACAACT+TGG 0.427192 1:+73339450 MsaT004745.1:CDS
CTGTTTGGTAAAGATCTGTT+AGG 0.427840 1:-73339489 None:intergenic
CCACTTCAATTTGAGGAAGA+AGG 0.429497 1:+73340453 MsaT004745.1:CDS
TTGAAAATAACTGACACATT+GGG 0.429946 1:+73340057 MsaT004745.1:CDS
AGTTTCACTCACCGGTGATT+CGG 0.439232 1:+73339510 MsaT004745.1:CDS
GATCAAGAGTAATTAATTTG+AGG 0.441411 1:+73339971 MsaT004745.1:CDS
AAGGTTAAGAATTGAGAAGA+AGG 0.444053 1:+73340239 MsaT004745.1:CDS
CTTGATAAGAAGGTTCTACT+AGG 0.446731 1:-73340141 None:intergenic
TTGCTGTGAAATGAGCAAAT+TGG 0.450939 1:-73339681 None:intergenic
CACCGGTGAGTGAAACTGTT+TGG 0.458694 1:-73339504 None:intergenic
AGGTTAGTTAGTTTCTCAAA+AGG 0.468291 1:+73339907 MsaT004745.1:CDS
AACTCCTATGAAGGCTATGA+AGG 0.469990 1:+73339316 MsaT004745.1:CDS
TTTGAGGAAGAAGGTGGTGG+TGG 0.477498 1:+73340462 MsaT004745.1:CDS
AGGGTTTCAGAAAGAGATGA+TGG 0.479941 1:+73340489 MsaT004745.1:CDS
TTCTTTCTCTATTAACTGAA+AGG 0.482856 1:-73339234 None:intergenic
ATTGAAAATAACTGACACAT+TGG 0.484927 1:+73340056 MsaT004745.1:CDS
TTATTGTCCACTTCAATTTG+AGG 0.488195 1:+73340446 MsaT004745.1:CDS
CTCAACATTGAAGATTCAAA+TGG 0.488668 1:+73339391 MsaT004745.1:CDS
GGAAACATGGAAAAGTAGAA+TGG 0.498264 1:+73340344 MsaT004745.1:CDS
AGCTTCAAGAAACTGAATCT+AGG 0.507835 1:+73339887 MsaT004745.1:CDS
AAAAGTAGAATGGAGAATCA+TGG 0.508498 1:+73340354 MsaT004745.1:CDS
CCTTCTTCCTCAAATTGAAG+TGG 0.513172 1:-73340453 None:intergenic
ACTAATAGAAACTCCTATGA+AGG 0.514783 1:+73339307 MsaT004745.1:CDS
AAATGAGCAAATTGGTAATA+CGG 0.517397 1:-73339673 None:intergenic
TACCAAACAGTTTCACTCAC+CGG 0.521272 1:+73339502 MsaT004745.1:CDS
GACACGTTGAACCGAATCAC+CGG 0.538056 1:-73339521 None:intergenic
AACCTTTCAATACTCTCAAG+TGG 0.550308 1:-73340222 None:intergenic
GTCTGTGAATCTTGATAAGA+AGG 0.567154 1:-73340151 None:intergenic
CAATTTGAGGAAGAAGGTGG+TGG 0.571559 1:+73340459 MsaT004745.1:CDS
GTTGTTGTTGTTGAACAAGA+AGG 0.574596 1:+73340108 MsaT004745.1:CDS
GTCGTCGCATATTTCACCGA+TGG 0.586765 1:+73339541 MsaT004745.1:CDS
TTTCAGAAAGAGATGATGGA+AGG 0.599854 1:+73340493 MsaT004745.1:CDS
GCTTAATAACTATGATGATG+TGG 0.611848 1:+73340287 MsaT004745.1:CDS
AATCAATAACATGAATAGAA+CGG 0.614448 1:-73339750 None:intergenic
TACCACTTGAGAGTATTGAA+AGG 0.618047 1:+73340220 MsaT004745.1:CDS
GAAAGTGATTGAATCAGTGA+AGG 0.619627 1:-73339799 None:intergenic
TTCAGAAAGAGATGATGGAA+GGG 0.626052 1:+73340494 MsaT004745.1:CDS
CTTCAATTTGAGGAAGAAGG+TGG 0.626263 1:+73340456 MsaT004745.1:CDS
GAAGGGCTATCTCTTTGGGA+TGG 0.632533 1:+73340511 MsaT004745.1:CDS
AATAACTATGATGATGTGGA+TGG 0.639203 1:+73340291 MsaT004745.1:CDS
AACTATGATGATGTGGATGG+TGG 0.649454 1:+73340294 MsaT004745.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 73339193 73340578 73339193 ID=MsaG004745
Chr1 mRNA 73339193 73340578 73339193 ID=MsaT004745.1;Parent=MsaG004745
Chr1 exon 73339193 73340578 73339193 ID=MsaT004745.1.exon1;Parent=MsaT004745.1
Chr1 CDS 73339193 73340578 73339193 ID=cds.MsaT004745.1;Parent=MsaT004745.1
Gene Sequence

>MsaT004745.1

ATGGAAGCTGATTCAGAAGAAGATGAGCTTCTGAATCTTAGCCTTTCAGTTAATAGAGAAAGAAAAAAGAAGGGAAAAATCATCATAAGTAGAGAAAATAACAACATGATGAGTACTAATAGAAACTCCTATGAAGGCTATGAAGGAAAAATCTTTCATCTTCTTCAAATGAGAGAACAAATGCTAAGAAAAAGCACTCTCAACATTGAAGATTCAAATGGTCTTCCATTAATTCACTTACTTCTCACAACAGCAACTTCTGTTGATGAAAACAACTTGGATTCATCTCTTGAAAACCTAACAGATCTTTACCAAACAGTTTCACTCACCGGTGATTCGGTTCAACGTGTCGTCGCATATTTCACCGATGGTTTAACTGCAAAACTCCTCACAAAAAAATCACCATTCTATGAAATGTTGATGGAAGAACCAACAATCGACGAAGAGTTTCTCGCATTCACAGATCTCTACAGAGTTTCACCGTATTACCAATTTGCTCATTTCACAGCAAATCAAGCTATCTTGGAAGCCTTTGAAAAAGAAGAAGAGAAAAATAACCGTTCTATTCATGTTATTGATTTTGATGTTTCATATGGTTTTCAATGGCCTTCACTGATTCAATCACTTTCAGAAAAAGCAACAAGTGGAAACAGAATCTCTTTCAGGTTAACTGGTTTCGGTAAGAATTTAAAAGAGCTTCAAGAAACTGAATCTAGGTTAGTTAGTTTCTCAAAAGGTTTTGGTAACATTGTTTTTGAGTTTCAAGGTTTGTTAAGAGGATCAAGAGTAATTAATTTGAGGAAGAAGAAAAATGAAACTGTGGCGGTTAATTTAGTTTCCTATTTAAACAAAATGAGTTGTTTATTGAAAATAACTGACACATTGGGATTTGTTCATTCTCTTAACCCTTCCATTGTTGTTGTTGTTGAACAAGAAGGTAGTAAAAATCCTAGTAGAACCTTCTTATCAAGATTCACAGACACATTGCATTATTTTGCAGCTATGTTTGATTCACTTGATGATTGTTTACCACTTGAGAGTATTGAAAGGTTAAGAATTGAGAAGAAGGTTTTTGGTAAAGAGATTAAAAGCATGCTTAATAACTATGATGATGTGGATGGTGGTGTTGATTGTGCAAAATATGAAAAAATGGAAACATGGAAAAGTAGAATGGAGAATCATGGATTTGTTGGTATGAAAATGAGTTCAAAATGTTTAATACAAGCAAAATTGTTGCTTAAGATGAGAACACATTATTGTCCACTTCAATTTGAGGAAGAAGGTGGTGGTGGTTTCAGGGTTTCAGAAAGAGATGATGGAAGGGCTATCTCTTTGGGATGGCAAAATAGGTTTTTGCTTACTGTGTCAGCATGGCAATCACTTTGA

Protein sequence

>MsaT004745.1

MEADSEEDELLNLSLSVNRERKKKGKIIISRENNNMMSTNRNSYEGYEGKIFHLLQMREQMLRKSTLNIEDSNGLPLIHLLLTTATSVDENNLDSSLENLTDLYQTVSLTGDSVQRVVAYFTDGLTAKLLTKKSPFYEMLMEEPTIDEEFLAFTDLYRVSPYYQFAHFTANQAILEAFEKEEEKNNRSIHVIDFDVSYGFQWPSLIQSLSEKATSGNRISFRLTGFGKNLKELQETESRLVSFSKGFGNIVFEFQGLLRGSRVINLRKKKNETVAVNLVSYLNKMSCLLKITDTLGFVHSLNPSIVVVVEQEGSKNPSRTFLSRFTDTLHYFAAMFDSLDDCLPLESIERLRIEKKVFGKEIKSMLNNYDDVDGGVDCAKYEKMETWKSRMENHGFVGMKMSSKCLIQAKLLLKMRTHYCPLQFEEEGGGGFRVSERDDGRAISLGWQNRFLLTVSAWQSL*