Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004779 | XP_014510477.1 | 31.684 | 849 | 412 | 26 | 30 | 750 | 55 | 863 | 5.23e-105 | 352 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004779 | sp|Q7XIT1|IRE1_ORYSJ | 36.184 | 456 | 251 | 12 | 306 | 750 | 462 | 888 | 2.37e-72 | 256 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004779 | tr|A0A1S3UWY7|A0A1S3UWY7_VIGRR | 31.684 | 849 | 412 | 26 | 30 | 750 | 55 | 863 | 2.50e-105 | 352 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004779 | MtrunA17_Chr8g0370241 | 36.424 | 453 | 232 | 11 | 329 | 750 | 417 | 844 | 4.47e-73 | 254 |
| MsaG004779 | MtrunA17_Chr8g0370241 | 35.714 | 266 | 95 | 9 | 19 | 210 | 21 | 284 | 2.07e-32 | 134 |
| MsaG004779 | MtrunA17_Chr5g0407261 | 36.947 | 452 | 226 | 14 | 331 | 750 | 476 | 900 | 3.14e-71 | 250 |
| MsaG004779 | MtrunA17_Chr5g0407261 | 35.176 | 199 | 80 | 5 | 31 | 184 | 60 | 254 | 2.57e-19 | 93.2 |
| MsaG004779 | MtrunA17_Chr8g0369971 | 41.392 | 273 | 139 | 7 | 329 | 601 | 363 | 614 | 2.18e-48 | 181 |
| MsaG004779 | MtrunA17_Chr8g0369971 | 46.766 | 201 | 87 | 6 | 19 | 201 | 17 | 215 | 1.21e-42 | 164 |
| MsaG004779 | MtrunA17_Chr3g0117811 | 25.078 | 319 | 193 | 12 | 342 | 635 | 65 | 362 | 2.62e-19 | 90.5 |
| MsaG004779 | MtrunA17_Chr5g0424581 | 29.537 | 281 | 156 | 10 | 341 | 611 | 129 | 377 | 3.77e-19 | 90.5 |
| MsaG004779 | MtrunA17_Chr3g0129721 | 29.391 | 279 | 162 | 12 | 343 | 612 | 31 | 283 | 8.81e-19 | 87.4 |
| MsaG004779 | MtrunA17_Chr5g0424651 | 29.537 | 281 | 156 | 10 | 341 | 611 | 325 | 573 | 1.17e-18 | 90.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004779 | AT2G17520.1 | 33.698 | 457 | 244 | 12 | 328 | 750 | 405 | 836 | 9.21e-69 | 243 |
| MsaG004779 | AT5G24360.1 | 33.259 | 448 | 239 | 11 | 334 | 749 | 456 | 875 | 1.85e-63 | 228 |
| MsaG004779 | AT5G24360.2 | 33.259 | 448 | 239 | 11 | 334 | 749 | 462 | 881 | 2.12e-63 | 228 |
| MsaG004779 | AT5G24360.3 | 33.259 | 448 | 239 | 11 | 334 | 749 | 442 | 861 | 2.15e-63 | 228 |
| MsaG004779 | AT3G11870.1 | 27.656 | 546 | 324 | 18 | 257 | 750 | 27 | 553 | 2.14e-43 | 166 |
| MsaG004779 | AT5G66210.6 | 30.795 | 302 | 161 | 17 | 326 | 609 | 48 | 319 | 8.29e-21 | 96.3 |
| MsaG004779 | AT5G66210.4 | 30.795 | 302 | 161 | 17 | 326 | 609 | 48 | 319 | 8.29e-21 | 96.3 |
| MsaG004779 | AT5G66210.5 | 30.795 | 302 | 161 | 17 | 326 | 609 | 48 | 319 | 9.56e-21 | 96.7 |
| MsaG004779 | AT5G66210.3 | 30.592 | 304 | 163 | 17 | 326 | 611 | 48 | 321 | 1.09e-20 | 96.7 |
| MsaG004779 | AT5G66210.1 | 30.795 | 302 | 161 | 17 | 326 | 609 | 48 | 319 | 1.92e-20 | 96.3 |
Find 165 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAGATAAAGAATGAGG+CGG | 0.680634 | 1:+73841725 | MsaT004779.1:CDS |
| AAATGAAGCTGAAGCTGGGC+AGG | 0.468781 | 1:+73842373 | MsaT004779.1:CDS |
| AACAATGAGAATGGTACAGT+TGG | 0.567611 | 1:+73839595 | MsaT004779.1:CDS |
| AACTGCACTAAATTCAGCGA+CGG | 0.624808 | 1:-73841361 | None:intergenic |
| AACTGGTCGACAAAGTTACA+TGG | 0.603319 | 1:+73844922 | MsaT004779.1:CDS |
| AACTGTACCATTCTCATTGT+TGG | 0.373854 | 1:-73839593 | None:intergenic |
| AAGACAGACACAGAATGAAG+CGG | 0.625410 | 1:+73841638 | MsaT004779.1:CDS |
| AAGCTAAGGATCTGATTAGC+CGG | 0.567402 | 1:+73844558 | MsaT004779.1:CDS |
| AAGGTCGTTCCTCAGAACAA+TGG | 0.592182 | 1:+73842437 | MsaT004779.1:CDS |
| AAGTACTCCCCACATGGTGT+TGG | 0.617710 | 1:-73841435 | None:intergenic |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 73839300 | 73845215 | 73839300 | ID=MsaG004779 |
| Chr1 | mRNA | 73839300 | 73845215 | 73839300 | ID=MsaT004779.1;Parent=MsaG004779 |
| Chr1 | exon | 73839300 | 73839388 | 73839300 | ID=MsaT004779.1.exon1;Parent=MsaT004779.1 |
| Chr1 | CDS | 73839300 | 73839388 | 73839300 | ID=cds.MsaT004779.1;Parent=MsaT004779.1 |
| Chr1 | exon | 73839534 | 73839747 | 73839534 | ID=MsaT004779.1.exon2;Parent=MsaT004779.1 |
| Chr1 | CDS | 73839534 | 73839747 | 73839534 | ID=cds.MsaT004779.1;Parent=MsaT004779.1 |
| Chr1 | exon | 73841147 | 73842207 | 73841147 | ID=MsaT004779.1.exon3;Parent=MsaT004779.1 |
| Chr1 | CDS | 73841147 | 73842207 | 73841147 | ID=cds.MsaT004779.1;Parent=MsaT004779.1 |
| Chr1 | exon | 73842292 | 73842469 | 73842292 | ID=MsaT004779.1.exon4;Parent=MsaT004779.1 |
| Chr1 | CDS | 73842292 | 73842469 | 73842292 | ID=cds.MsaT004779.1;Parent=MsaT004779.1 |
| Chr1 | exon | 73844350 | 73844606 | 73844350 | ID=MsaT004779.1.exon5;Parent=MsaT004779.1 |
| Chr1 | CDS | 73844350 | 73844606 | 73844350 | ID=cds.MsaT004779.1;Parent=MsaT004779.1 |
| Chr1 | exon | 73844762 | 73845215 | 73844762 | ID=MsaT004779.1.exon6;Parent=MsaT004779.1 |
| Chr1 | CDS | 73844762 | 73845215 | 73844762 | ID=cds.MsaT004779.1;Parent=MsaT004779.1 |
| Gene Sequence |
>MsaT004779.1 ATGAAAGGTCTCATAGGTCTCTGGTTTCTGATCCTTTTCCTTACGTGGCTATTGCAAGACTGTTGCTGCTTCCATCCTTTTCCTTCTCCTGAGAAGGCAACAGCAACGGCACTCTTTGCCGAACCTGATGGCACCATTTATTTGGTCGCCAACAATGAGAATGGTACAGTTGGAGTGTTATGGTCATTCTCCACAGGATCACCGATTTACAGCGCATATCACTCTGCTGGTACTGAAAGATACTTGTTTTATGAGGGTGGTGATTGGTCATTGTTTACCGAGGATCCGCACTACAAAAAATTGATAGTTGCTGAATCAATTGCGTCTTTTGTTGATAACACGCCTAGAAATGATGGAGTAGGCCCTGTCCATGGTCGCCACAACAGCACACTGTTTGTAGTTGATGCTGAGACAGGGTCACTTAATACGAGTACGGAGCCTGTAATGTACGTCTATAGGAAGGATTACTTTTTGTACTCCGAAAGAAGCAGACCAAAAACAGGATTTTGGAATATGTCCGTCGCTGAATTTAGTGCAGTTTGCAAATGCCAGAAGGCAATTTTTCATGTCAGCAACAAACGTTTTATAATGCCAACACCATGTGGGGAGTACTTTAACGTGAGGCGAATCACAAACGTATCAGAAGTTTATATGCCTCCAAGTTATGTTGGTTTTAACATGATGGGTTCAATAATATATACAGTCTATGTTATTGGACTATCACTTGGGCTATTTTTTTCTCTGTGCCTTTGTTCTCGGGTTCTTGGAGGCGTATCAAACGATGCGAATGCGGAAAGACAGACACAGAATGAAGCGGAAGAACAGAAGACGAATGAGGAGGCAGAAGATCTGCAAGCAGAAGTGGAAGAACAGGATGCGGAAAAACAGATAAAGAATGAGGCGGAAGAACAGATGACGAAGGAGGCAGAAGATCTAAAAGCAGTAGTGAAAGAACATACAACAAAGGGCAAGCCAAAAAGATCGGATCTTAAAACTTTTGGCAATATTGAGGTGTCAAATGAAGTGATTGGACAAGGCAGTCATGGTTCTCTTGTTTATGCTGGATACTTTAAGCCTTCGGATAAAGAAATTGACAAGCAAGAAGTTGCAGTTAAACGGCTTATCCGCGGCCATCAATCTCTAGCTGATAAAGAGATAAGAATACTCTTGAAATTGAAAAGTCATGGAAACGTTGTAAGATACTTTGGGAGTGCAGAGTGTGATGATTTTGTGTACCTTGCAACTGAAAGGTGTTGGGTGAACTTGTATGAGTTCATCGAAAAAGAAAAAGGGGGAGGTGCTGAATTTGATTTGTGGAATAAGATTACCGGTCAACCATCAGCCTTGTTAATGAAACTTATGATGGGGCTTGTGAAGGGGATACGGTTTTTGCACGACAAGAAAATTATCCATAGAGATTTGAAACCACAAAACATTCTGTTAAAAAATGAAGCTGAAGCTGGGCAGGATAAGTCATTGGTCATCAAGATCTCAGATATGGGCATAAGCAAGGTCGTTCCTCAGAACAATGGGAATGGTACTACAGGCACTCGGGGTTGGCGATCCCCAGAGCAGATTAAGGGAGGAACCCCCCATAGTTATCCGTCTGATATCTTTAGCTTGGGGATCACAATGTTTTACTGTTTGACCAAGGGACACCATCCATTTGGAGATATTGCAAATGAGGGTGAGACTGAGATTTCAATATTGAACAATAAGATGGATCTCATGTTACTTGATGGCTTAGGGGAAGCTAAGGATCTGATTAGCCGGCTATTAATCTCGGACCCATCACAGAGGCTGGTGGCGGATCAGGTGTTTAAACATCTTTTTTTCTGGGATTCAGAAAAGAGATTACATTTTGTAGAGGAAGTTCATACCGTCTTGAGTGGCAAAAAGAAGGGATCTCTCCTGAAATTATTTGAACAACAGACTTGCCGGTTACCATTACTTAAAGGAAACTGGTCGACAAAGTTACATGGAGGTTTGAAGAAGGAAGCTCGAAAACCCTATGATCGTTATGATAATCCTCGTCCCTACGACCTTACGAAAGTTGCCGGTTTAGTCCGTTTCATAAGAAACAAATCTGTTCATAGGAAGAGCAACGAAACAGCGATTAAGAACGTAATTGGAGAGACATTTGAATCAGTTGATGGTTACTTTAGGAAACGTTTTCCAGAATTGATACCGTCGCTGTACAGTTTTGTGATTAGCCACTGCAAGGGGGATAATGCTTTTAAAGTCTATTTCTAG |
| Protein sequence |
>MsaT004779.1 MKGLIGLWFLILFLTWLLQDCCCFHPFPSPEKATATALFAEPDGTIYLVANNENGTVGVLWSFSTGSPIYSAYHSAGTERYLFYEGGDWSLFTEDPHYKKLIVAESIASFVDNTPRNDGVGPVHGRHNSTLFVVDAETGSLNTSTEPVMYVYRKDYFLYSERSRPKTGFWNMSVAEFSAVCKCQKAIFHVSNKRFIMPTPCGEYFNVRRITNVSEVYMPPSYVGFNMMGSIIYTVYVIGLSLGLFFSLCLCSRVLGGVSNDANAERQTQNEAEEQKTNEEAEDLQAEVEEQDAEKQIKNEAEEQMTKEAEDLKAVVKEHTTKGKPKRSDLKTFGNIEVSNEVIGQGSHGSLVYAGYFKPSDKEIDKQEVAVKRLIRGHQSLADKEIRILLKLKSHGNVVRYFGSAECDDFVYLATERCWVNLYEFIEKEKGGGAEFDLWNKITGQPSALLMKLMMGLVKGIRFLHDKKIIHRDLKPQNILLKNEAEAGQDKSLVIKISDMGISKVVPQNNGNGTTGTRGWRSPEQIKGGTPHSYPSDIFSLGITMFYCLTKGHHPFGDIANEGETEISILNNKMDLMLLDGLGEAKDLISRLLISDPSQRLVADQVFKHLFFWDSEKRLHFVEEVHTVLSGKKKGSLLKLFEQQTCRLPLLKGNWSTKLHGGLKKEARKPYDRYDNPRPYDLTKVAGLVRFIRNKSVHRKSNETAIKNVIGETFESVDGYFRKRFPELIPSLYSFVISHCKGDNAFKVYF* |
