AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004789

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004789 MtrunA17_Chr5g0407261 42.523 214 107 6 55 263 615 817 9.57e-40 147
MsaG004789 MtrunA17_Chr8g0370241 46.821 173 80 4 55 224 558 721 2.03e-38 143
MsaG004789 MtrunA17_Chr8g0369971 44.295 149 70 4 55 202 479 615 2.40e-27 110
MsaG004789 MtrunA17_Chr2g0326531 31.765 170 102 5 46 213 222 379 7.65e-19 85.5
MsaG004789 MtrunA17_Chr2g0333821 32.934 167 98 5 51 214 113 268 1.41e-15 76.3
MsaG004789 MtrunA17_Chr3g0115171 28.177 181 116 5 36 214 368 536 2.31e-15 75.9
MsaG004789 MtrunA17_Chr5g0428031 31.073 177 98 6 48 217 106 265 9.87e-15 73.6
MsaG004789 MtrunA17_Chr3g0129721 32.000 175 101 7 46 213 119 282 3.06e-14 71.2
MsaG004789 MtrunA17_Chr3g0132671 28.824 170 104 4 48 214 146 301 3.74e-14 72.0
MsaG004789 MtrunA17_Chr3g0114511 28.488 172 109 5 46 214 124 284 5.89e-14 71.2
MsaG004789 MtrunA17_Chr5g0424501 32.500 160 90 8 58 213 299 444 7.59e-14 71.2
MsaG004789 MtrunA17_Chr4g0029241 28.497 193 117 7 24 208 193 372 7.89e-14 70.9
MsaG004789 MtrunA17_Chr4g0059181 33.945 109 57 4 58 164 218 313 1.71e-13 69.7
MsaG004789 MtrunA17_Chr3g0117811 33.945 109 57 4 58 164 189 284 2.28e-13 69.3
MsaG004789 MtrunA17_Chr5g0432471 29.448 163 83 5 58 208 118 260 2.58e-13 69.7
MsaG004789 MtrunA17_Chr6g0453681 27.778 180 112 4 42 214 103 271 6.21e-13 68.6
MsaG004789 MtrunA17_Chr1g0150321 28.916 166 96 7 51 209 112 262 7.01e-13 68.2
MsaG004789 MtrunA17_Chr1g0188991 29.651 172 95 6 55 214 119 276 7.64e-13 68.6
MsaG004789 MtrunA17_Chr6g0468751 27.778 180 112 4 42 214 103 271 7.79e-13 68.2
MsaG004789 MtrunA17_Chr1g0205421 27.907 172 110 5 46 214 106 266 1.17e-12 67.4
MsaG004789 MtrunA17_Chr2g0286501 28.743 167 102 5 58 218 150 305 1.25e-12 67.8
MsaG004789 MtrunA17_Chr4g0000481 30.357 168 93 6 58 214 185 339 1.28e-12 67.0
MsaG004789 MtrunA17_Chr5g0424581 29.762 168 84 6 58 213 232 377 1.83e-12 67.0
MsaG004789 MtrunA17_Chr1g0211611 34.711 121 66 5 44 164 393 500 2.04e-12 67.0
MsaG004789 MtrunA17_Chr5g0402091 29.940 167 101 5 52 213 109 264 2.15e-12 66.6
MsaG004789 MtrunA17_Chr4g0000431 28.655 171 98 5 55 214 128 285 3.59e-12 66.2
MsaG004789 MtrunA17_Chr3g0090261 27.815 151 95 6 54 202 188 326 4.60e-12 65.5
MsaG004789 MtrunA17_Chr5g0424651 27.429 175 113 5 41 213 411 573 5.22e-12 65.9
MsaG004789 MtrunA17_Chr6g0450571 28.488 172 97 6 55 214 120 277 5.27e-12 65.9
MsaG004789 MtrunA17_Chr1g0188031 29.885 174 106 8 48 217 119 280 6.29e-12 65.5
MsaG004789 MtrunA17_Chr6g0474861 39.823 113 51 5 55 160 390 492 6.99e-12 65.5
MsaG004789 MtrunA17_Chr3g0107051 26.404 178 113 5 51 223 120 284 1.14e-11 64.7
MsaG004789 MtrunA17_Chr6g0474811 39.823 113 51 5 55 160 677 779 1.15e-11 65.1
MsaG004789 MtrunA17_Chr5g0424531 28.571 175 111 5 41 213 106 268 1.25e-11 63.5
MsaG004789 MtrunA17_Chr6g0474831 40.000 100 45 4 55 149 160 249 1.49e-11 63.5
MsaG004789 MtrunA17_Chr2g0304111 27.746 173 101 5 50 214 122 278 1.53e-11 64.3
MsaG004789 MtrunA17_Chr5g0432491 28.652 178 109 6 51 223 123 287 1.69e-11 63.9
MsaG004789 MtrunA17_Chr6g0474871 39.823 113 51 5 55 160 669 771 1.79e-11 64.3
MsaG004789 MtrunA17_Chr1g0202101 32.479 117 61 5 45 154 368 473 1.86e-11 63.9
MsaG004789 MtrunA17_Chr2g0317001 28.022 182 112 4 39 219 109 272 2.39e-11 63.9
MsaG004789 MtrunA17_Chr8g0346071 27.976 168 105 4 50 214 116 270 2.47e-11 63.5
MsaG004789 MtrunA17_Chr8g0375141 30.286 175 88 8 52 213 145 298 2.67e-11 63.2
MsaG004789 MtrunA17_Chr4g0008561 28.704 216 131 10 58 260 125 330 3.02e-11 63.5
MsaG004789 MtrunA17_Chr8g0361441 37.692 130 60 6 32 150 581 700 3.30e-11 63.5
MsaG004789 MtrunA17_Chr8g0384301 29.814 161 100 4 58 216 408 557 4.03e-11 63.2
MsaG004789 MtrunA17_Chr6g0474901 40.000 100 45 4 55 149 670 759 4.28e-11 63.2
MsaG004789 MtrunA17_Chr4g0051191 26.344 186 103 6 41 214 254 417 5.06e-11 62.8
MsaG004789 MtrunA17_Chr1g0166101 31.765 170 101 7 51 217 125 282 5.42e-11 62.4
MsaG004789 MtrunA17_Chr4g0069301 28.402 169 101 7 52 214 127 281 6.03e-11 62.4
MsaG004789 MtrunA17_Chr8g0353621 31.250 160 92 7 55 211 114 258 6.04e-11 61.6
MsaG004789 MtrunA17_Chr2g0302431 28.402 169 101 7 52 214 121 275 6.67e-11 62.4
MsaG004789 MtrunA17_Chr4g0052981 29.310 174 91 7 52 213 106 259 6.93e-11 62.0
MsaG004789 MtrunA17_Chr6g0449691 30.682 176 91 8 55 218 188 344 9.87e-11 62.0
MsaG004789 MtrunA17_Chr6g0475091 40.594 101 45 4 55 150 685 775 9.99e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004789 AT2G17520.1 39.151 212 117 4 55 263 551 753 2.76e-37 140
MsaG004789 AT5G24360.1 36.744 215 118 6 55 262 589 792 2.44e-33 129
MsaG004789 AT5G24360.2 36.744 215 118 6 55 262 595 798 2.69e-33 129
MsaG004789 AT5G24360.3 36.744 215 118 6 55 262 575 778 2.78e-33 129
MsaG004789 AT3G11870.1 36.571 175 98 5 55 224 252 418 3.53e-23 99.0
MsaG004789 AT5G60550.1 30.000 200 122 7 51 244 215 402 2.64e-17 81.6
MsaG004789 AT5G01810.3 30.682 176 90 6 51 214 111 266 1.20e-16 79.3
MsaG004789 AT5G01810.1 30.682 176 90 6 51 214 111 266 1.35e-16 79.3
MsaG004789 AT5G01810.2 30.682 176 90 6 51 214 111 266 1.35e-16 79.3
MsaG004789 AT3G45240.2 30.675 163 99 5 51 211 216 366 3.37e-16 78.2
MsaG004789 AT3G45240.1 30.675 163 99 5 51 211 216 366 3.37e-16 78.2
MsaG004789 AT1G45160.1 34.500 200 81 8 51 214 770 955 5.14e-16 78.6
MsaG004789 AT1G45160.2 34.500 200 81 8 51 214 770 955 6.00e-16 78.2
MsaG004789 AT3G63260.1 27.273 187 112 7 44 219 196 369 8.70e-16 77.0
MsaG004789 AT3G29160.1 29.353 201 124 4 42 235 104 293 7.18e-15 74.7
MsaG004789 AT3G29160.2 29.353 201 124 4 42 235 104 293 7.18e-15 74.7
MsaG004789 AT3G29160.3 29.353 201 124 4 42 235 104 293 7.43e-15 73.9
MsaG004789 AT1G12580.1 32.353 170 97 8 48 211 142 299 1.44e-14 73.9
MsaG004789 AT1G48490.4 32.273 220 101 10 42 224 920 1128 1.64e-14 73.9
MsaG004789 AT1G48490.3 32.273 220 101 10 42 224 919 1127 1.76e-14 73.9
MsaG004789 AT1G48490.2 32.273 220 101 10 42 224 919 1127 1.76e-14 73.9
MsaG004789 AT1G48490.1 32.273 220 101 10 42 224 919 1127 1.76e-14 73.9
MsaG004789 AT3G63260.2 31.538 130 67 5 44 164 196 312 3.20e-14 72.0
MsaG004789 AT3G45240.3 30.061 163 97 6 51 211 216 363 3.87e-14 72.0
MsaG004789 AT5G50000.2 30.247 162 90 5 13 164 158 306 4.06e-14 71.6
MsaG004789 AT5G50000.1 30.247 162 90 5 13 164 158 306 6.87e-14 71.2
MsaG004789 AT5G39440.1 32.121 165 100 5 50 214 118 270 1.03e-13 71.2
MsaG004789 AT5G40440.4 30.208 192 102 9 58 236 190 362 1.76e-13 70.5
MsaG004789 AT5G40440.1 30.208 192 102 9 58 236 190 362 1.76e-13 70.5
MsaG004789 AT5G40440.6 30.208 192 102 9 58 236 172 344 1.83e-13 70.5
MsaG004789 AT5G40440.3 30.208 192 102 9 58 236 172 344 1.83e-13 70.5
MsaG004789 AT5G40440.2 30.208 192 102 9 58 236 172 344 1.83e-13 70.5
MsaG004789 AT5G40440.5 30.208 192 102 9 58 236 108 280 1.97e-13 70.1
MsaG004789 AT1G30270.3 29.762 168 104 5 50 214 33 189 2.01e-13 70.1
MsaG004789 AT1G30270.2 29.762 168 104 5 50 214 130 286 2.61e-13 70.1
MsaG004789 AT1G30270.1 28.736 174 98 5 50 214 130 286 3.26e-13 69.7
MsaG004789 AT4G14580.1 30.636 173 106 6 48 217 119 280 3.43e-13 69.3
MsaG004789 AT4G24400.1 30.052 193 111 8 48 233 106 281 3.47e-13 69.7
MsaG004789 AT3G01490.2 33.945 109 57 4 58 164 237 332 3.62e-13 69.3
MsaG004789 AT4G24400.2 30.052 193 111 8 48 233 106 281 4.11e-13 69.3
MsaG004789 AT3G01490.1 33.945 109 57 4 58 164 237 332 5.33e-13 68.9
MsaG004789 AT4G08500.1 31.111 180 101 6 41 213 423 586 7.78e-13 68.6
MsaG004789 AT3G01090.3 27.363 201 128 4 42 235 103 292 7.85e-13 68.6
MsaG004789 AT3G01090.1 27.363 201 128 4 42 235 103 292 7.85e-13 68.6
MsaG004789 AT1G53165.3 30.061 163 103 3 58 219 120 272 8.40e-13 68.6
MsaG004789 AT1G53165.2 30.061 163 103 3 58 219 120 272 9.28e-13 68.6
MsaG004789 AT3G01090.2 27.363 201 128 4 42 235 126 315 1.01e-12 68.2
MsaG004789 AT4G30960.1 27.222 180 107 6 51 222 123 286 1.05e-12 68.2
MsaG004789 AT4G10730.1 29.240 171 97 5 55 214 151 308 1.13e-12 68.2
MsaG004789 AT1G53165.1 30.061 163 103 3 58 219 120 272 1.15e-12 68.2
MsaG004789 AT4G10730.2 29.651 172 95 6 55 214 131 288 1.17e-12 68.2
MsaG004789 AT3G17850.1 29.204 226 100 10 52 231 983 1194 1.41e-12 68.2
MsaG004789 AT3G15220.1 28.834 163 105 3 58 219 120 272 1.76e-12 67.8
MsaG004789 AT1G48260.2 30.588 170 104 6 48 214 23 181 3.66e-12 66.2
MsaG004789 AT1G48260.4 30.588 170 104 6 48 214 23 181 3.66e-12 66.2
MsaG004789 AT1G48260.3 30.588 170 104 6 48 214 23 181 3.66e-12 66.2
MsaG004789 AT1G48260.5 30.588 170 104 6 48 214 23 181 3.66e-12 66.2
MsaG004789 AT1G78290.3 28.161 174 104 7 50 214 99 260 4.81e-12 65.9
MsaG004789 AT1G78290.2 28.161 174 104 7 50 214 99 260 4.81e-12 65.9
MsaG004789 AT1G48260.1 30.588 170 104 6 48 214 108 266 5.28e-12 65.9
MsaG004789 AT1G01140.2 27.647 170 107 5 50 214 118 276 5.70e-12 65.9
MsaG004789 AT2G37840.1 30.409 171 100 6 55 218 115 273 7.59e-12 65.9
MsaG004789 AT5G14720.1 27.907 172 98 6 55 214 120 277 7.79e-12 65.9
MsaG004789 AT5G14720.2 27.907 172 98 6 55 214 120 277 7.79e-12 65.9
MsaG004789 AT5G25110.1 29.341 167 104 5 51 214 142 297 8.86e-12 65.5
MsaG004789 AT4G35780.1 33.333 120 67 5 46 164 384 491 9.00e-12 65.5
MsaG004789 AT1G79640.2 30.994 171 94 6 55 214 117 274 9.93e-12 65.5
MsaG004789 AT1G79640.4 30.994 171 94 6 55 214 117 274 9.93e-12 65.5
MsaG004789 AT1G79640.3 30.994 171 94 6 55 214 117 274 9.93e-12 65.5
MsaG004789 AT1G79640.5 30.994 171 94 6 55 214 120 277 1.02e-11 65.5
MsaG004789 AT1G79640.1 30.994 171 94 6 55 214 117 274 1.06e-11 65.5
MsaG004789 AT1G12680.1 31.098 164 89 8 62 216 212 360 1.13e-11 65.1
MsaG004789 AT5G45820.1 29.167 168 103 6 51 214 111 266 1.19e-11 65.1
MsaG004789 AT1G79640.6 30.994 171 94 6 55 214 117 274 1.26e-11 65.1
MsaG004789 AT4G24100.3 29.070 172 96 6 55 214 153 310 1.38e-11 65.1
MsaG004789 AT4G24100.4 29.070 172 96 6 55 214 153 310 1.40e-11 65.1
MsaG004789 AT4G24100.1 29.070 172 96 6 55 214 137 294 1.40e-11 65.1
MsaG004789 AT4G24100.2 29.070 172 96 6 55 214 137 294 1.42e-11 65.1
MsaG004789 AT3G21220.2 30.357 168 93 7 58 214 171 325 1.49e-11 64.3
MsaG004789 AT3G21220.1 30.357 168 93 7 58 214 171 325 1.49e-11 64.3
MsaG004789 AT2G17700.1 33.613 119 66 6 47 164 379 485 1.58e-11 64.7
MsaG004789 AT5G55090.1 28.161 174 90 8 52 213 105 255 1.63e-11 64.7
MsaG004789 AT4G33950.2 27.586 174 109 6 48 214 114 277 1.94e-11 63.9
MsaG004789 AT2G24360.1 27.545 167 107 5 54 218 236 390 2.29e-11 63.9
MsaG004789 AT5G55090.2 28.000 175 89 8 52 213 167 317 2.46e-11 63.9
MsaG004789 AT4G33950.1 27.586 174 109 6 48 214 114 277 2.92e-11 63.5
MsaG004789 AT3G17510.2 31.325 166 94 7 46 205 35 186 3.29e-11 63.5
MsaG004789 AT4G31170.7 26.190 168 110 5 53 218 236 391 3.33e-11 63.5
MsaG004789 AT4G31170.6 26.190 168 110 5 53 218 236 391 3.33e-11 63.5
MsaG004789 AT4G31170.5 26.190 168 110 5 53 218 236 391 3.33e-11 63.5
MsaG004789 AT4G31170.1 26.190 168 110 5 53 218 236 391 3.33e-11 63.5
MsaG004789 AT4G31170.2 26.190 168 110 5 53 218 236 391 3.33e-11 63.5
MsaG004789 AT4G31170.3 26.190 168 110 5 53 218 236 391 3.33e-11 63.5
MsaG004789 AT4G31170.8 26.190 168 110 5 53 218 236 391 3.33e-11 63.5
MsaG004789 AT2G38910.1 31.792 173 97 9 47 211 230 389 4.68e-11 63.5
MsaG004789 AT3G17510.1 31.325 166 94 7 46 205 115 266 4.86e-11 63.2
MsaG004789 AT1G01140.3 27.059 170 106 5 50 214 118 274 6.02e-11 62.8
MsaG004789 AT2G26980.2 29.630 162 82 6 50 199 113 254 6.23e-11 62.0
MsaG004789 AT1G01140.1 27.059 170 106 5 50 214 118 274 6.32e-11 62.8
MsaG004789 AT3G06030.1 33.333 165 92 7 61 219 183 335 6.33e-11 63.2
MsaG004789 AT1G51660.1 30.952 168 92 7 58 214 180 334 6.96e-11 62.4
MsaG004789 AT2G38490.1 31.579 114 66 3 51 162 127 230 7.48e-11 62.4
MsaG004789 AT5G56580.1 29.310 174 100 8 58 220 176 337 7.97e-11 62.4

Find 65 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTTACCATTACTTGTTAA+AGG 0.206476 1:-74015031 MsaT004789.1:CDS
GGTCATCAAGATCTCGGATA+TGG 0.283671 1:-74016458 MsaT004789.1:CDS
GCGATCCCCAGAGCAGATTA+AGG 0.292814 1:-74015547 MsaT004789.1:CDS
TCTGTTGTTCAAATAATTTC+CGG 0.295999 1:+74015059 None:intergenic
TGCCAGTGATCCTTCATATT+GGG 0.319704 1:+74019306 None:intergenic
GTTCCTCCCTTAATCTGCTC+TGG 0.327065 1:+74015540 None:intergenic
CATTACTTGTTAAAGGAAAC+TGG 0.330094 1:-74015024 MsaT004789.1:CDS
CCGTCTGATATCTTTAGCTT+GGG 0.337290 1:-74015504 MsaT004789.1:CDS
AGATATCAGACGGATAACTA+TGG 0.344947 1:+74015514 None:intergenic
TTGCCAGTGATCCTTCATAT+TGG 0.345601 1:+74019305 None:intergenic
ATGGATCTCATGTTACTTGA+TGG 0.368910 1:-74015393 MsaT004789.1:CDS
ACCAAGGGCCACCATCCATT+TGG 0.370338 1:-74015459 MsaT004789.1:CDS
TTCCTCCCTTAATCTGCTCT+GGG 0.373672 1:+74015541 None:intergenic
TCATGTTACTTGATGGCTTA+GGG 0.375395 1:-74015386 MsaT004789.1:CDS
TATTTGAACAACAGACTTGC+TGG 0.384296 1:-74015051 MsaT004789.1:CDS
TCCGTCTGATATCTTTAGCT+TGG 0.390459 1:-74015505 MsaT004789.1:CDS
CTCATGTTACTTGATGGCTT+AGG 0.399684 1:-74015387 MsaT004789.1:CDS
CAGTGAATATTCTGACTCTC+AGG 0.402877 1:+74019339 None:intergenic
TTTGTGGTTTCGGATCTCTA+TGG 0.436360 1:+74016530 None:intergenic
CAGTTTCCTTTAACAAGTAA+TGG 0.437225 1:+74015025 None:intergenic
TTCAATATTGAACAATAAGA+TGG 0.452974 1:-74015412 MsaT004789.1:CDS
TGCTCTGGGGATCGCCAACC+CGG 0.458871 1:+74015555 None:intergenic
CGATCCCCAGAGCAGATTAA+GGG 0.475727 1:-74015546 MsaT004789.1:CDS
TGAGGAAGGAAGTTACTGTA+GGG 0.476823 1:-74014979 MsaT004789.1:CDS
TTTACCTCTGAGATGGGTCC+GGG 0.480588 1:+74015331 None:intergenic
ACTTATTTCCAGGCTGGTGG+CGG 0.483692 1:-74015182 MsaT004789.1:intron
AGGTCGTTCTTCAGAACAAT+GGG 0.486817 1:-74016426 MsaT004789.1:CDS
GAAAGTTACATGGAGATTTG+AGG 0.486850 1:-74014997 MsaT004789.1:CDS
AGCTGAGCAGCATAACTCAT+TGG 0.494981 1:-74016479 MsaT004789.1:CDS
GTCATCAAGATCTCGGATAT+GGG 0.497481 1:-74016457 MsaT004789.1:CDS
TCCAAATGGATGGTGGCCCT+TGG 0.498824 1:+74015458 None:intergenic
TTGAGGAAGGAAGTTACTGT+AGG 0.501085 1:-74014980 MsaT004789.1:CDS
CTCATTGGTCATCAAGATCT+CGG 0.502816 1:-74016464 MsaT004789.1:CDS
GATATCAGACGGATAACTAT+GGG 0.506588 1:+74015515 None:intergenic
ATTGCAGATACAGGCACTCC+GGG 0.513292 1:-74015573 MsaT004789.1:intron
AACAATGGGAATGGTACCCT+TGG 0.527260 1:-74016412 MsaT004789.1:intron
GTTACATGGAGATTTGAGGA+AGG 0.528819 1:-74014993 MsaT004789.1:CDS
TCCACTTATTTCCAGGCTGG+TGG 0.529612 1:-74015185 MsaT004789.1:intron
CTCCCAATATGAAGGATCAC+TGG 0.537051 1:-74019308 MsaT004789.1:CDS
CAGATACAGGCACTCCGGGT+TGG 0.550562 1:-74015569 MsaT004789.1:CDS
TCTTATGAAACGCACTAACT+CGG 0.557152 1:+74014950 None:intergenic
TCAACATGCTCCCAATATGA+AGG 0.558292 1:-74019316 MsaT004789.1:CDS
CGTCTGATATCTTTAGCTTG+GGG 0.559976 1:-74015503 MsaT004789.1:CDS
GATTAGCCGGCTATTAATCC+CGG 0.563917 1:-74015349 MsaT004789.1:CDS
AAGCAAAGGATCTGATTAGC+CGG 0.568657 1:-74015362 MsaT004789.1:CDS
AATTGCAGATACAGGCACTC+CGG 0.570463 1:-74015574 MsaT004789.1:intron
CATGTTACTTGATGGCTTAG+GGG 0.579639 1:-74015385 MsaT004789.1:CDS
AAGGTCGTTCTTCAGAACAA+TGG 0.580530 1:-74016427 MsaT004789.1:CDS
TTGTAAGATCTCCAAATGGA+TGG 0.603112 1:+74015448 None:intergenic
TGATGGCTTAGGGGAAGCAA+AGG 0.606786 1:-74015376 MsaT004789.1:CDS
ATGGGTCCGGGATTAATAGC+CGG 0.607532 1:+74015343 None:intergenic
CCCAAGCTAAAGATATCAGA+CGG 0.620366 1:+74015504 None:intergenic
CTCGGATATGGGAATAAGCA+AGG 0.623739 1:-74016446 MsaT004789.1:CDS
AGTGAATATTCTGACTCTCA+GGG 0.625573 1:+74019340 None:intergenic
GTTCTTCAGAACAATGGGAA+TGG 0.629454 1:-74016421 MsaT004789.1:CDS
TATCAGACGGATAACTATGG+GGG 0.631502 1:+74015517 None:intergenic
AACTGGTCGAGAAAGTTACA+TGG 0.634761 1:-74015007 MsaT004789.1:CDS
TCAGTTGTAAGATCTCCAAA+TGG 0.637105 1:+74015444 None:intergenic
ACTCTCAGGGATCGTCGAAG+AGG 0.639374 1:+74019353 None:intergenic
TCCCCAGAGCAGATTAAGGG+AGG 0.661857 1:-74015543 MsaT004789.1:CDS
TAAGATCTCCAAATGGATGG+TGG 0.665872 1:+74015451 None:intergenic
TCCTCCCTTAATCTGCTCTG+GGG 0.676537 1:+74015542 None:intergenic
ATATCAGACGGATAACTATG+GGG 0.679510 1:+74015516 None:intergenic
ATCAGACGGATAACTATGGG+GGG 0.706674 1:+74015518 None:intergenic
TAATCCCGGACCCATCTCAG+AGG 0.711253 1:-74015335 MsaT004789.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 74014964 74019430 74014964 ID=MsaG004789
Chr1 mRNA 74014964 74019430 74014964 ID=MsaT004789.1;Parent=MsaG004789
Chr1 exon 74014964 74015192 74014964 ID=MsaT004789.1.exon4;Parent=MsaT004789.1
Chr1 CDS 74014964 74015192 74014964 ID=cds.MsaT004789.1;Parent=MsaT004789.1
Chr1 exon 74015336 74015588 74015336 ID=MsaT004789.1.exon3;Parent=MsaT004789.1
Chr1 CDS 74015336 74015588 74015336 ID=cds.MsaT004789.1;Parent=MsaT004789.1
Chr1 exon 74016413 74016597 74016413 ID=MsaT004789.1.exon2;Parent=MsaT004789.1
Chr1 CDS 74016413 74016597 74016413 ID=cds.MsaT004789.1;Parent=MsaT004789.1
Chr1 exon 74019264 74019430 74019264 ID=MsaT004789.1.exon1;Parent=MsaT004789.1
Chr1 CDS 74019264 74019430 74019264 ID=cds.MsaT004789.1;Parent=MsaT004789.1
Gene Sequence

>MsaT004789.1

ATGGCTATTTTTGAGATGATTGCTTTTGAGAGGGTTGGGATGATTGTGTACACAGCCTCTTCGACGATCCCTGAGAGTCAGAATATTCACTGTCAACATGCTCCCAATATGAAGGATCACTGGCAAATATTTTACATATACAAGACTAAAACTCGTCTGCAAATACAGGGGATTGTGAAGGGGATACGGTTTTTGCACGGAAAGAAAATTATCCATAGAGATCCGAAACCACAAAACATTCTGTTGTTAAAAAATGAAGCTGAAGCTGAGCAGCATAACTCATTGGTCATCAAGATCTCGGATATGGGAATAAGCAAGGTCGTTCTTCAGAACAATGGGAATGGTACCCTTGATACAGGCACTCCGGGTTGGCGATCCCCAGAGCAGATTAAGGGAGGAACCCCCCATAGTTATCCGTCTGATATCTTTAGCTTGGGGATCACAATGTTTTACTGTTTGACCAAGGGCCACCATCCATTTGGAGATCTTACAACTGAGCGTGAGAATTCAATATTGAACAATAAGATGGATCTCATGTTACTTGATGGCTTAGGGGAAGCAAAGGATCTGATTAGCCGGCTATTAATCCCGGACCCATCTCAGAGGCTGGTGGCGGATCAAGTGTTGAAACATCTTTTTTTCTGGGATTCAGAACAGAGATTACGTTTTGTATGGGCAGTTCATACCGTCTTGAGTGACAAAAAGAAAGGATCTCTCCGGAAATTATTTGAACAACAGACTTGCTGGTTACCATTACTTGTTAAAGGAAACTGGTCGAGAAAGTTACATGGAGATTTGAGGAAGGAAGTTACTGTAGGGAAGTTGCCGAGTTAG
Protein sequence

>MsaT004789.1

MAIFEMIAFERVGMIVYTASSTIPESQNIHCQHAPNMKDHWQIFYIYKTKTRLQIQGIVKGIRFLHGKKIIHRDPKPQNILLLKNEAEAEQHNSLVIKISDMGISKVVLQNNGNGTLDTGTPGWRSPEQIKGGTPHSYPSDIFSLGITMFYCLTKGHHPFGDLTTERENSILNNKMDLMLLDGLGEAKDLISRLLIPDPSQRLVADQVLKHLFFWDSEQRLRFVWAVHTVLSDKKKGSLRKLFEQQTCWLPLLVKGNWSRKLHGDLRKEVTVGKLPS*