Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004918 | XP_039690635.1 | 83.333 | 282 | 39 | 1 | 1 | 282 | 1 | 274 | 1.72e-169 | 498 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004918 | sp|Q9LKR4|FRS10_ARATH | 29.891 | 184 | 123 | 4 | 96 | 276 | 67 | 247 | 1.65e-19 | 92.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004918 | tr|A0A2K3MAC5|A0A2K3MAC5_TRIPR | 70.922 | 282 | 75 | 2 | 1 | 282 | 1 | 275 | 5.25e-139 | 407 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG004918 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000605 | MsaG004918 | 0.804995 | 2.792473e-49 | 4.233049e-47 |
| MsaG001371 | MsaG004918 | 0.838432 | 5.308477e-57 | 1.973117e-54 |
| MsaG003384 | MsaG004918 | 0.821076 | 8.594341e-53 | 1.944831e-50 |
| MsaG004514 | MsaG004918 | 0.810800 | 1.648761e-50 | 2.871061e-48 |
| MsaG004656 | MsaG004918 | 0.823412 | 2.481070e-53 | 5.977130e-51 |
| MsaG004681 | MsaG004918 | 0.821607 | 6.489061e-53 | 1.489192e-50 |
| MsaG004811 | MsaG004918 | 0.898418 | 1.234431e-76 | 5.759457e-73 |
| MsaG004918 | MsaG005394 | 0.820956 | 9.156526e-53 | 2.065468e-50 |
| MsaG004918 | MsaG006924 | 0.812203 | 8.196169e-51 | 1.477204e-48 |
| MsaG004918 | MsaG007092 | 0.805708 | 1.983028e-49 | 3.056323e-47 |
| MsaG004918 | MsaG009121 | 0.816068 | 1.160367e-51 | 2.304590e-49 |
| MsaG004918 | MsaG009396 | 0.810657 | 1.769922e-50 | 3.071264e-48 |
| MsaG004918 | MsaG010149 | 0.805275 | 2.441840e-49 | 3.725587e-47 |
| MsaG004918 | MsaG011861 | 0.818931 | 2.646387e-52 | 5.658794e-50 |
| MsaG004918 | MsaG012163 | 0.831759 | 2.511836e-55 | 7.648493e-53 |
| MsaG004918 | MsaG012615 | 0.837726 | 8.048231e-57 | 2.926935e-54 |
| MsaG004918 | MsaG015958 | 0.801408 | 1.530149e-48 | 2.135736e-46 |
| MsaG004918 | MsaG016550 | 0.834989 | 3.969143e-56 | 1.329059e-53 |
| MsaG004918 | MsaG018025 | 0.803629 | 5.358943e-49 | 7.870144e-47 |
| MsaG004918 | MsaG018631 | 0.809558 | 3.044789e-50 | 5.144561e-48 |
| MsaG004918 | MsaG020430 | 0.808330 | 5.559363e-50 | 9.119099e-48 |
| MsaG004918 | MsaG021564 | 0.824665 | 1.264575e-53 | 3.152046e-51 |
| MsaG004918 | MsaG023404 | 0.809580 | 3.012414e-50 | 5.092577e-48 |
| MsaG004918 | MsaG025893 | 0.806983 | 1.071010e-49 | 1.701112e-47 |
| MsaG004918 | MsaG026719 | 0.839782 | 2.381265e-57 | 9.232499e-55 |
| MsaG004918 | MsaG027241 | 0.825958 | 6.274340e-54 | 1.620760e-51 |
| MsaG004918 | MsaG028248 | 0.802782 | 8.008222e-49 | 1.153314e-46 |
| MsaG004918 | MsaG029547 | 0.817831 | 4.683882e-52 | 9.733949e-50 |
| MsaG004918 | MsaG039902 | 0.800073 | 2.857520e-48 | 3.869909e-46 |
| MsaG004918 | MsaG042710 | 0.817992 | 4.309644e-52 | 8.993495e-50 |
| MsaG004918 | MsaG043371 | 0.832260 | 1.891206e-55 | 5.842681e-53 |
| MsaG004918 | MsaG045276 | 0.800339 | 2.523805e-48 | 3.438536e-46 |
| MsaG004918 | MsaG046436 | 0.817067 | 6.948126e-52 | 1.415813e-49 |
| MsaG004918 | MsaG004075 | 0.801574 | 1.415779e-48 | 1.983494e-46 |
| MsaG004918 | MsaG012729 | 0.834655 | 4.810938e-56 | 1.594996e-53 |
| MsaG004918 | MsaG028280 | 0.836088 | 2.098001e-56 | 7.260529e-54 |
| MsaG004918 | MsaG033146 | 0.821346 | 7.448597e-53 | 1.697591e-50 |
| MsaG004918 | MsaG032930 | 0.825892 | 6.505059e-54 | 1.677246e-51 |
| MsaG004918 | MsaG037155 | 0.815517 | 1.537352e-51 | 3.010969e-49 |
| MsaG004918 | MsaG038926 | 0.806791 | 1.175503e-49 | 1.858641e-47 |
| MsaG004918 | MsaG037511 | 0.822774 | 3.489889e-53 | 8.263939e-51 |
| MsaG004918 | MsaG041727 | 0.843213 | 3.000311e-58 | 1.297084e-55 |
| MsaG004918 | MsaG043822 | 0.828141 | 1.895623e-54 | 5.204531e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004918 | MtrunA17_Chr1g0203051 | 59.929 | 282 | 108 | 2 | 1 | 282 | 1 | 277 | 2.67e-119 | 356 |
| MsaG004918 | MtrunA17_Chr8g0389811 | 46.377 | 138 | 74 | 0 | 58 | 195 | 58 | 195 | 2.32e-45 | 152 |
| MsaG004918 | MtrunA17_Chr2g0287651 | 31.797 | 217 | 144 | 2 | 75 | 288 | 66 | 281 | 3.97e-35 | 134 |
| MsaG004918 | MtrunA17_Chr2g0331371 | 44.961 | 129 | 71 | 0 | 64 | 192 | 35 | 163 | 2.62e-32 | 119 |
| MsaG004918 | MtrunA17_Chr1g0171741 | 34.437 | 151 | 98 | 1 | 64 | 214 | 113 | 262 | 2.46e-28 | 109 |
| MsaG004918 | MtrunA17_Chr6g0473421 | 38.519 | 135 | 69 | 2 | 33 | 159 | 2 | 130 | 3.43e-28 | 106 |
| MsaG004918 | MtrunA17_Chr8g0340801 | 47.727 | 88 | 46 | 0 | 195 | 282 | 1 | 88 | 1.28e-22 | 90.1 |
| MsaG004918 | MtrunA17_Chr1g0172931 | 41.818 | 110 | 64 | 0 | 57 | 166 | 49 | 158 | 3.28e-21 | 87.8 |
| MsaG004918 | MtrunA17_Chr2g0290391 | 26.866 | 201 | 132 | 4 | 96 | 282 | 63 | 262 | 2.45e-17 | 82.0 |
| MsaG004918 | MtrunA17_Chr1g0198761 | 31.088 | 193 | 115 | 7 | 96 | 274 | 59 | 247 | 2.91e-16 | 79.0 |
| MsaG004918 | MtrunA17_Chr2g0276841 | 35.075 | 134 | 80 | 4 | 90 | 218 | 76 | 207 | 6.32e-16 | 77.8 |
| MsaG004918 | MtrunA17_Chr5g0431001 | 29.167 | 240 | 146 | 10 | 50 | 269 | 21 | 256 | 3.24e-15 | 75.5 |
| MsaG004918 | MtrunA17_Chr5g0448761 | 27.673 | 159 | 106 | 3 | 91 | 240 | 60 | 218 | 2.82e-12 | 67.0 |
| MsaG004918 | MtrunA17_Chr8g0366311 | 45.763 | 59 | 32 | 0 | 57 | 115 | 52 | 110 | 4.59e-12 | 61.6 |
| MsaG004918 | MtrunA17_Chr7g0258861 | 26.901 | 171 | 116 | 3 | 91 | 254 | 10 | 178 | 7.74e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG004918 | AT5G28530.1 | 29.891 | 184 | 123 | 4 | 96 | 276 | 67 | 247 | 1.68e-20 | 92.0 |
| MsaG004918 | AT5G28530.3 | 29.891 | 184 | 123 | 4 | 96 | 276 | 67 | 247 | 1.69e-20 | 92.0 |
| MsaG004918 | AT5G28530.2 | 29.891 | 184 | 123 | 4 | 96 | 276 | 67 | 247 | 1.69e-20 | 92.0 |
| MsaG004918 | AT5G28530.5 | 29.891 | 184 | 123 | 4 | 96 | 276 | 67 | 247 | 1.76e-20 | 91.7 |
| MsaG004918 | AT5G28530.4 | 29.891 | 184 | 123 | 4 | 96 | 276 | 67 | 247 | 1.79e-20 | 91.7 |
| MsaG004918 | AT1G10240.2 | 28.495 | 186 | 119 | 7 | 90 | 267 | 54 | 233 | 2.94e-14 | 73.6 |
| MsaG004918 | AT1G10240.1 | 28.495 | 186 | 119 | 7 | 90 | 267 | 54 | 233 | 2.94e-14 | 73.6 |
| MsaG004918 | AT4G38180.1 | 28.105 | 153 | 107 | 3 | 90 | 240 | 79 | 230 | 6.69e-11 | 63.2 |
Find 56 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACACGTACAATTCGTTCTA+TGG | 0.271258 | 1:-75774699 | MsaT004918.1:CDS |
| CAACTCCACGATCCGGAAAA+TGG | 0.292979 | 1:+75774583 | None:intergenic |
| CTTTGAGAAAATTAATTTCA+AGG | 0.360382 | 1:-75774634 | MsaT004918.1:CDS |
| ATTCCTACGTCACGTATCTA+TGG | 0.365780 | 1:-75774195 | MsaT004918.1:CDS |
| GGTTGTGTGCATGGATTTGC+TGG | 0.371733 | 1:-75774537 | MsaT004918.1:CDS |
| ATGTTCCTCGGATGATGATA+TGG | 0.372317 | 1:-75774724 | MsaT004918.1:CDS |
| AACTAGTAGGTGTGGGTGTA+TGG | 0.373550 | 1:-75774388 | MsaT004918.1:CDS |
| TCAAACGTGAGCAACATAAC+TGG | 0.389168 | 1:-75774937 | MsaT004918.1:CDS |
| ACTGGGGAAGGCAACATAGC+TGG | 0.398079 | 1:-75774919 | MsaT004918.1:CDS |
| CTGGGGAAGGCAACATAGCT+GGG | 0.441129 | 1:-75774918 | MsaT004918.1:CDS |
| CTTGAATTGCTCATTGTACA+TGG | 0.453134 | 1:+75774119 | None:intergenic |
| ACCCTAGAGATGACATTCAT+TGG | 0.453922 | 1:-75773114 | MsaT004918.1:intron |
| GGATCGCAGTAGTTCTTGTT+CGG | 0.473684 | 1:-75774757 | MsaT004918.1:CDS |
| TCACTCATCTTTCTATGTGC+AGG | 0.492964 | 1:+75774258 | None:intergenic |
| ATGAAAGCAATGAGAAAAGC+TGG | 0.503092 | 1:-75774219 | MsaT004918.1:CDS |
| TTGAATGCAACTCCACGATC+CGG | 0.516695 | 1:+75774576 | None:intergenic |
| AATTTCTGAAGGGATTGTCT+AGG | 0.518783 | 1:-75774056 | MsaT004918.1:CDS |
| GGACGGACGATATGATGTAT+TGG | 0.519058 | 1:-75774035 | MsaT004918.1:CDS |
| GAGAGCACAAGCCAACTAGT+AGG | 0.519908 | 1:-75774401 | MsaT004918.1:CDS |
| CAAACGTGAGCAACATAACT+GGG | 0.524047 | 1:-75774936 | MsaT004918.1:CDS |
| ACCTTTGTTTGTCATAGAGA+AGG | 0.539558 | 1:-75774465 | MsaT004918.1:CDS |
| TATTGACATTCCTTGCATCC+CGG | 0.539899 | 1:+75774439 | None:intergenic |
| CACAAGCCAACTAGTAGGTG+TGG | 0.542797 | 1:-75774396 | MsaT004918.1:CDS |
| CATGTACAATGAGCAATTCA+AGG | 0.545333 | 1:-75774118 | MsaT004918.1:CDS |
| TTTGTCATAGAGAAGGTCTC+CGG | 0.545529 | 1:-75774458 | MsaT004918.1:CDS |
| GGAATGTCAATAATTTGTCT+AGG | 0.547272 | 1:-75774428 | MsaT004918.1:CDS |
| AATATGTACGGTTGTGTGCA+TGG | 0.553072 | 1:-75774546 | MsaT004918.1:CDS |
| CATAGCTGGGGAAGGTTCAG+AGG | 0.556178 | 1:-75774905 | MsaT004918.1:intron |
| TGATGCAATTGAATTTCTGA+AGG | 0.556876 | 1:-75774067 | MsaT004918.1:CDS |
| AAAATCTAGGATATTCAAGA+AGG | 0.557216 | 1:-75774143 | MsaT004918.1:CDS |
| CAATGAGCAATTCAAGGAGA+GGG | 0.559489 | 1:-75774112 | MsaT004918.1:CDS |
| ACACGTACAATTCGTTCTAT+GGG | 0.559891 | 1:-75774698 | MsaT004918.1:CDS |
| GAGAAGGTCTCCGGGATGCA+AGG | 0.564819 | 1:-75774449 | MsaT004918.1:CDS |
| ATACACCCACACCTACTAGT+TGG | 0.569760 | 1:+75774390 | None:intergenic |
| GATGCAATTGAATTTCTGAA+GGG | 0.581745 | 1:-75774066 | MsaT004918.1:CDS |
| TAGAGATGACATTCATTGGA+CGG | 0.586970 | 1:-75773110 | MsaT004918.1:intron |
| CGTGTTGTTAAGAACAAGGA+TGG | 0.592808 | 1:-75774504 | MsaT004918.1:CDS |
| GAAGGCAACATAGCTGGGGA+AGG | 0.594260 | 1:-75774913 | MsaT004918.1:CDS |
| TTGTCATAGAGAAGGTCTCC+GGG | 0.600219 | 1:-75774457 | MsaT004918.1:CDS |
| ATTCAAGTACACGTTGACTA+CGG | 0.603567 | 1:-75774360 | MsaT004918.1:CDS |
| CGGACGATATGATGTATTGG+AGG | 0.618358 | 1:-75774032 | MsaT004918.1:CDS |
| CATCCTTGTTCTTAACAACA+CGG | 0.626313 | 1:+75774505 | None:intergenic |
| ACCTTCTCTATGACAAACAA+AGG | 0.626355 | 1:+75774464 | None:intergenic |
| ATGCCGTGTTGTTAAGAACA+AGG | 0.641432 | 1:-75774508 | MsaT004918.1:CDS |
| ACAAGCCAACTAGTAGGTGT+GGG | 0.642158 | 1:-75774395 | MsaT004918.1:CDS |
| GTCTTCCATATCATCATCCG+AGG | 0.646493 | 1:+75774719 | None:intergenic |
| GTGAGCAACATAACTGGGGA+AGG | 0.649113 | 1:-75774931 | MsaT004918.1:CDS |
| ATGAGCAATTCAAGGAGAGG+GGG | 0.649130 | 1:-75774110 | MsaT004918.1:CDS |
| TCTGAAGGGATTGTCTAGGA+CGG | 0.649575 | 1:-75774052 | MsaT004918.1:CDS |
| TGGGGAAGGCAACATAGCTG+GGG | 0.651748 | 1:-75774917 | MsaT004918.1:CDS |
| ACAATGAGCAATTCAAGGAG+AGG | 0.651900 | 1:-75774113 | MsaT004918.1:CDS |
| AATGAGCAATTCAAGGAGAG+GGG | 0.664675 | 1:-75774111 | MsaT004918.1:CDS |
| ACGTTGACTACGGTAGTGAT+CGG | 0.671418 | 1:-75774350 | MsaT004918.1:CDS |
| AAACCATAGATACGTGACGT+AGG | 0.700125 | 1:+75774192 | None:intergenic |
| TTGACTACGGTAGTGATCGG+TGG | 0.725493 | 1:-75774347 | MsaT004918.1:CDS |
| AAACGTGAGCAACATAACTG+GGG | 0.752985 | 1:-75774935 | MsaT004918.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 75773086 | 75774995 | 75773086 | ID=MsaG004918 |
| Chr1 | mRNA | 75773086 | 75774995 | 75773086 | ID=MsaT004918.1;Parent=MsaG004918 |
| Chr1 | exon | 75773086 | 75773127 | 75773086 | ID=MsaT004918.1.exon3;Parent=MsaT004918.1 |
| Chr1 | CDS | 75773086 | 75773127 | 75773086 | ID=cds.MsaT004918.1;Parent=MsaT004918.1 |
| Chr1 | exon | 75774026 | 75774778 | 75774026 | ID=MsaT004918.1.exon2;Parent=MsaT004918.1 |
| Chr1 | CDS | 75774026 | 75774778 | 75774026 | ID=cds.MsaT004918.1;Parent=MsaT004918.1 |
| Chr1 | exon | 75774906 | 75774995 | 75774906 | ID=MsaT004918.1.exon1;Parent=MsaT004918.1 |
| Chr1 | CDS | 75774906 | 75774995 | 75774906 | ID=cds.MsaT004918.1;Parent=MsaT004918.1 |
| Gene Sequence |
| Protein sequence |
