AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004942

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004942 MtrunA17_Chr1g0202461 94.551 312 16 1 1 311 77 388 0.0 514
MsaG004942 MtrunA17_Chr8g0348311 67.544 114 34 2 160 271 132 244 4.25e-46 159
MsaG004942 MtrunA17_Chr2g0293481 55.738 122 40 2 164 276 238 354 1.27e-37 139
MsaG004942 MtrunA17_Chr8g0387501 44.809 183 84 7 137 311 84 257 1.60e-35 129
MsaG004942 MtrunA17_Chr7g0270051 40.260 154 70 2 147 292 8 147 5.64e-32 119
MsaG004942 MtrunA17_Chr2g0289181 49.074 108 52 2 173 277 3 110 1.88e-31 117
MsaG004942 MtrunA17_Chr8g0379461 42.446 139 65 3 123 259 26 151 5.92e-28 108
MsaG004942 MtrunA17_Chr2g0284321 56.522 92 33 2 163 248 9 99 2.00e-27 106
MsaG004942 MtrunA17_Chr5g0401821 58.824 85 29 2 163 242 174 257 7.31e-26 103
MsaG004942 MtrunA17_Chr4g0053281 54.118 85 34 1 163 242 240 324 6.55e-25 102
MsaG004942 MtrunA17_Chr2g0319961 52.747 91 33 1 164 244 50 140 8.41e-25 100
MsaG004942 MtrunA17_Chr1g0184691 67.797 59 19 0 185 243 294 352 4.14e-23 97.8
MsaG004942 MtrunA17_Chr4g0071521 51.807 83 35 1 165 242 221 303 7.85e-23 96.3
MsaG004942 MtrunA17_Chr1g0147881 48.750 80 40 1 162 241 241 319 1.49e-21 93.2
MsaG004942 MtrunA17_Chr7g0228941 52.459 61 29 0 181 241 49 109 8.43e-20 83.6
MsaG004942 MtrunA17_Chr7g0218081 45.652 92 49 1 158 249 384 474 9.04e-20 89.7
MsaG004942 MtrunA17_Chr7g0218081 43.077 65 35 2 180 244 224 286 1.05e-11 65.5
MsaG004942 MtrunA17_Chr4g0074001 45.783 83 43 2 166 247 340 421 4.94e-19 87.4
MsaG004942 MtrunA17_Chr3g0139021 44.578 83 45 1 159 241 290 371 7.14e-19 86.3
MsaG004942 MtrunA17_Chr7g0249311 44.048 84 45 2 166 248 373 455 1.64e-17 83.2
MsaG004942 MtrunA17_Chr7g0249311 45.205 73 38 2 172 244 167 237 1.03e-14 74.7
MsaG004942 MtrunA17_Chr3g0099641 38.835 103 61 1 181 281 248 350 3.36e-17 81.6
MsaG004942 MtrunA17_Chr7g0238921 51.429 70 31 2 172 241 114 180 1.09e-16 79.3
MsaG004942 MtrunA17_Chr4g0042831 45.455 77 40 2 172 247 265 340 2.33e-16 79.0
MsaG004942 MtrunA17_Chr4g0042831 41.176 68 39 1 177 244 138 204 8.98e-12 65.1
MsaG004942 MtrunA17_Chr5g0418191 44.156 77 41 2 172 247 307 382 2.99e-16 79.0
MsaG004942 MtrunA17_Chr5g0418191 42.647 68 37 2 177 244 144 209 1.52e-11 64.7
MsaG004942 MtrunA17_Chr2g0302391 43.678 87 49 0 184 270 211 297 3.42e-16 78.6
MsaG004942 MtrunA17_Chr7g0270831 46.575 73 39 0 182 254 214 286 3.50e-16 78.6
MsaG004942 MtrunA17_Chr5g0447701 40.217 92 53 2 170 260 237 327 4.37e-16 78.2
MsaG004942 MtrunA17_Chr5g0447701 39.080 87 51 2 158 244 66 150 7.20e-12 65.5
MsaG004942 MtrunA17_Chr7g0263531 44.737 76 40 2 179 254 184 257 4.98e-16 78.2
MsaG004942 MtrunA17_Chr7g0263531 40.816 98 42 3 162 244 324 420 1.76e-15 76.6
MsaG004942 MtrunA17_Chr3g0079691 42.353 85 47 2 166 249 476 559 5.50e-16 78.6
MsaG004942 MtrunA17_Chr3g0079691 49.315 73 34 3 183 254 279 349 3.02e-13 70.1
MsaG004942 MtrunA17_Chr7g0249301 52.381 63 29 1 185 247 293 354 9.74e-16 77.0
MsaG004942 MtrunA17_Chr7g0249301 45.205 73 38 2 172 244 50 120 2.83e-15 75.5
MsaG004942 MtrunA17_Chr1g0194481 49.275 69 33 2 185 252 159 226 1.19e-15 76.3
MsaG004942 MtrunA17_Chr4g0004911 42.169 83 46 2 166 247 317 398 1.71e-15 76.6
MsaG004942 MtrunA17_Chr4g0004911 32.000 125 69 2 185 309 185 293 2.11e-13 70.5
MsaG004942 MtrunA17_Chr4g0051881 48.649 74 37 1 185 258 179 251 2.24e-15 75.5
MsaG004942 MtrunA17_Chr3g0106491 38.542 96 57 2 166 260 346 440 4.99e-15 75.5
MsaG004942 MtrunA17_Chr3g0106491 44.444 63 33 2 183 245 197 257 1.94e-11 64.7
MsaG004942 MtrunA17_Chr2g0322391 50.000 64 32 0 181 244 346 409 5.07e-15 75.5
MsaG004942 MtrunA17_Chr3g0101131 49.206 63 31 1 185 247 416 477 5.84e-15 75.5
MsaG004942 MtrunA17_Chr3g0101131 38.961 77 45 2 177 253 236 310 3.27e-11 63.9
MsaG004942 MtrunA17_Chr5g0445311 46.479 71 34 2 185 252 365 434 6.25e-15 75.1
MsaG004942 MtrunA17_Chr2g0288871 49.206 63 32 0 182 244 303 365 8.47e-15 74.7
MsaG004942 MtrunA17_Chr5g0443131 52.459 61 28 1 185 245 158 217 8.52e-15 73.6
MsaG004942 MtrunA17_Chr4g0001361 41.333 75 44 0 182 256 160 234 1.03e-14 73.6
MsaG004942 MtrunA17_Chr5g0418781 42.222 90 44 3 158 241 93 180 1.21e-14 72.8
MsaG004942 MtrunA17_Chr4g0025051 45.205 73 40 0 182 254 333 405 1.22e-14 74.3
MsaG004942 MtrunA17_Chr8g0383251 39.759 83 47 2 164 244 86 167 1.28e-14 70.9
MsaG004942 MtrunA17_Chr5g0432161 41.176 85 48 2 166 249 402 485 1.28e-14 74.3
MsaG004942 MtrunA17_Chr5g0432161 48.387 62 30 2 183 244 243 302 5.95e-12 66.2
MsaG004942 MtrunA17_Chr7g0243891 37.624 101 60 3 163 262 352 450 1.53e-14 73.9
MsaG004942 MtrunA17_Chr7g0243891 45.161 62 32 2 183 244 215 274 7.97e-12 65.9
MsaG004942 MtrunA17_Chr1g0150801 52.632 57 26 1 185 241 103 158 1.57e-14 70.1
MsaG004942 MtrunA17_Chr8g0387981 41.176 85 43 2 162 239 105 189 2.33e-14 72.8
MsaG004942 MtrunA17_Chr3g0126391 52.632 57 25 1 185 239 134 190 2.37e-14 72.8
MsaG004942 MtrunA17_Chr3g0136961 38.824 85 52 0 185 269 139 223 2.41e-14 72.0
MsaG004942 MtrunA17_Chr4g0051181 50.820 61 30 0 185 245 244 304 2.54e-14 73.2
MsaG004942 MtrunA17_Chr4g0075201 37.168 113 57 2 185 283 268 380 3.44e-14 72.8
MsaG004942 MtrunA17_Chr4g0025061 43.836 73 41 0 182 254 358 430 4.82e-14 72.4
MsaG004942 MtrunA17_Chr7g0270191 40.860 93 53 2 185 276 146 237 6.45e-14 70.9
MsaG004942 MtrunA17_Chr4g0068541 45.902 61 32 1 185 245 209 268 7.03e-14 71.6
MsaG004942 MtrunA17_Chr3g0129321 46.667 60 31 1 185 244 119 177 7.58e-14 69.3
MsaG004942 MtrunA17_Chr7g0216891 38.835 103 58 2 152 254 192 289 1.09e-13 71.6
MsaG004942 MtrunA17_Chr4g0060271 50.000 60 30 0 185 244 256 315 1.14e-13 71.6
MsaG004942 MtrunA17_Chr2g0294231 47.541 61 31 1 185 245 204 263 1.37e-13 70.9
MsaG004942 MtrunA17_Chr2g0333851 35.644 101 62 1 185 285 170 267 2.45e-13 69.3
MsaG004942 MtrunA17_Chr8g0335811 40.506 79 45 2 165 242 134 211 2.61e-13 68.2
MsaG004942 MtrunA17_Chr1g0150281 36.842 76 48 0 185 260 141 216 4.00e-13 68.6
MsaG004942 MtrunA17_Chr1g0189391 42.553 94 43 5 172 255 84 176 5.19e-13 68.6
MsaG004942 MtrunA17_Chr8g0347251 35.514 107 57 3 163 257 110 216 9.73e-13 67.8
MsaG004942 MtrunA17_Chr1g0150271 33.962 106 68 2 185 289 102 206 1.39e-12 66.6
MsaG004942 MtrunA17_Chr7g0275531 44.928 69 32 2 185 247 119 187 3.38e-12 66.2
MsaG004942 MtrunA17_Chr5g0447711 44.286 70 38 1 178 247 224 292 3.63e-12 65.9
MsaG004942 MtrunA17_Chr7g0224801 42.623 61 34 1 181 241 90 149 5.97e-12 63.2
MsaG004942 MtrunA17_Chr6g0485431 49.180 61 29 2 185 244 155 214 6.19e-12 65.5
MsaG004942 MtrunA17_Chr8g0383351 46.552 58 30 1 185 242 161 217 9.22e-12 63.9
MsaG004942 MtrunA17_Chr5g0428351 41.250 80 40 2 162 239 166 240 1.52e-11 64.3
MsaG004942 MtrunA17_Chr3g0129361 46.552 58 30 1 185 242 145 201 1.69e-11 62.8
MsaG004942 MtrunA17_Chr1g0188941 42.623 61 34 1 185 245 52 111 6.33e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004942 AT5G52830.1 75.229 109 23 1 162 266 143 251 4.04e-53 177
MsaG004942 AT4G01250.1 40.000 265 87 8 1 257 1 201 1.26e-47 162
MsaG004942 AT4G23550.1 62.745 102 38 0 169 270 125 226 5.78e-39 139
MsaG004942 AT1G30650.1 46.067 178 67 5 156 305 177 353 2.62e-36 135
MsaG004942 AT2G34830.1 56.757 111 39 1 151 252 173 283 3.48e-36 135
MsaG004942 AT2G34830.2 56.757 111 39 1 151 252 182 292 3.68e-36 135
MsaG004942 AT5G45050.2 43.716 183 85 4 140 310 1114 1290 7.20e-33 129
MsaG004942 AT5G45050.1 43.716 183 85 4 140 310 1142 1318 7.23e-33 129
MsaG004942 AT5G45050.4 43.716 183 85 4 140 310 1003 1179 7.41e-33 129
MsaG004942 AT1G29280.1 65.278 72 20 1 184 255 74 140 1.05e-25 103
MsaG004942 AT4G24240.1 58.824 85 29 2 163 242 256 339 1.55e-25 104
MsaG004942 AT3G58710.2 58.537 82 26 1 184 257 70 151 1.72e-25 103
MsaG004942 AT3G58710.1 58.537 82 26 1 184 257 71 152 1.74e-25 103
MsaG004942 AT2G23320.1 52.381 84 36 1 163 242 215 298 6.11e-25 102
MsaG004942 AT5G28650.1 67.797 59 19 0 185 243 263 321 1.15e-23 99.4
MsaG004942 AT5G45260.1 49.505 101 47 3 161 258 1185 1284 1.31e-23 101
MsaG004942 AT3G04670.1 67.797 59 19 0 185 243 263 321 2.32e-23 98.6
MsaG004942 AT2G24570.1 51.220 82 38 1 162 241 219 300 3.10e-22 95.1
MsaG004942 AT4G31550.1 50.000 82 39 1 162 241 222 303 4.46e-22 94.7
MsaG004942 AT4G31550.2 48.780 82 40 1 162 241 221 302 1.47e-21 93.6
MsaG004942 AT2G30590.1 50.000 82 40 1 162 243 292 372 1.66e-20 91.3
MsaG004942 AT4G30935.1 36.364 132 79 3 168 295 314 444 2.53e-18 85.5
MsaG004942 AT2G04880.1 44.565 92 44 3 165 250 287 377 8.38e-18 84.0
MsaG004942 AT2G04880.2 44.565 92 44 3 165 250 263 353 8.77e-18 84.0
MsaG004942 AT4G26440.1 43.158 95 49 3 166 256 353 446 1.03e-16 80.9
MsaG004942 AT4G26440.1 47.059 85 38 4 173 256 171 249 5.79e-15 75.9
MsaG004942 AT3G01080.2 53.125 64 29 1 181 244 341 403 1.90e-16 80.1
MsaG004942 AT3G01080.2 42.308 78 43 2 180 257 201 276 1.01e-13 71.6
MsaG004942 AT3G01080.1 53.125 64 29 1 181 244 303 365 2.10e-16 79.7
MsaG004942 AT3G01080.1 42.308 78 43 2 180 257 163 238 1.12e-13 71.6
MsaG004942 AT5G56270.1 43.373 83 45 2 166 247 468 549 4.52e-16 79.3
MsaG004942 AT5G56270.1 48.333 60 29 2 185 244 274 331 4.08e-12 67.4
MsaG004942 AT1G13960.1 41.176 85 48 2 161 244 385 468 6.70e-16 78.6
MsaG004942 AT1G13960.1 34.862 109 60 4 141 244 185 287 7.99e-13 69.3
MsaG004942 AT4G04450.2 39.326 89 54 0 182 270 196 284 6.86e-16 78.2
MsaG004942 AT1G13960.2 44.595 74 39 2 172 244 243 315 9.58e-16 77.4
MsaG004942 AT1G13960.2 34.862 109 60 4 141 244 32 134 1.98e-12 67.4
MsaG004942 AT1G69310.4 43.333 90 43 3 158 241 116 203 1.00e-15 76.3
MsaG004942 AT1G69310.2 43.333 90 43 3 158 241 116 203 1.00e-15 76.3
MsaG004942 AT1G69310.1 43.333 90 43 3 158 241 116 203 1.00e-15 76.3
MsaG004942 AT2G38470.1 39.091 110 62 3 172 277 349 457 1.02e-15 77.8
MsaG004942 AT2G38470.1 36.290 124 68 4 185 302 185 303 6.64e-13 69.3
MsaG004942 AT4G04450.1 39.326 89 54 0 182 270 290 378 1.34e-15 77.4
MsaG004942 AT2G03340.1 43.038 79 43 2 172 249 402 479 1.64e-15 77.4
MsaG004942 AT2G03340.1 42.308 78 43 2 179 256 245 320 4.65e-13 70.1
MsaG004942 AT3G62340.1 35.115 131 80 3 163 289 95 224 2.05e-15 75.5
MsaG004942 AT5G07100.1 39.796 98 53 3 164 256 213 309 2.15e-15 75.9
MsaG004942 AT5G07100.1 41.176 68 39 1 177 244 110 176 1.61e-12 67.4
MsaG004942 AT2G21900.1 40.449 89 45 2 164 245 82 169 3.34e-15 73.6
MsaG004942 AT5G07100.2 39.796 98 53 3 164 256 120 216 3.51e-15 73.6
MsaG004942 AT5G07100.2 41.176 68 39 1 177 244 17 83 1.05e-12 66.6
MsaG004942 AT5G07100.4 39.796 98 53 3 164 256 120 216 3.51e-15 73.6
MsaG004942 AT5G07100.4 41.176 68 39 1 177 244 17 83 1.05e-12 66.6
MsaG004942 AT5G07100.5 39.796 98 53 3 164 256 120 216 3.51e-15 73.6
MsaG004942 AT5G07100.5 41.176 68 39 1 177 244 17 83 1.05e-12 66.6
MsaG004942 AT5G07100.3 39.796 98 53 3 164 256 120 216 3.51e-15 73.6
MsaG004942 AT5G07100.3 41.176 68 39 1 177 244 17 83 1.05e-12 66.6
MsaG004942 AT4G22070.4 49.206 63 32 0 182 244 295 357 4.09e-15 76.3
MsaG004942 AT4G22070.3 49.206 63 32 0 182 244 295 357 4.09e-15 76.3
MsaG004942 AT4G22070.2 49.206 63 32 0 182 244 295 357 4.09e-15 76.3
MsaG004942 AT4G22070.1 49.206 63 32 0 182 244 295 357 4.09e-15 76.3
MsaG004942 AT1G29860.1 43.590 78 40 2 185 262 137 210 4.42e-15 74.7
MsaG004942 AT2G40740.1 41.667 120 53 5 160 269 143 255 4.98e-15 74.3
MsaG004942 AT2G40740.3 41.667 120 53 5 160 269 152 264 5.26e-15 74.7
MsaG004942 AT1G68150.1 48.000 75 38 1 185 259 236 309 5.41e-15 75.1
MsaG004942 AT4G01720.1 53.333 60 28 0 185 244 240 299 5.71e-15 75.5
MsaG004942 AT5G26170.1 51.724 58 27 1 185 242 114 170 5.91e-15 72.0
MsaG004942 AT2G37260.1 45.882 85 39 3 162 241 323 405 6.04e-15 75.5
MsaG004942 AT2G37260.1 41.935 93 52 2 178 270 159 249 1.65e-14 73.9
MsaG004942 AT1G62300.1 49.206 63 32 0 182 244 310 372 7.11e-15 75.5
MsaG004942 AT2G37260.3 45.882 85 39 3 162 241 243 325 7.21e-15 74.7
MsaG004942 AT2G37260.3 41.935 93 52 2 178 270 79 169 1.66e-14 73.6
MsaG004942 AT5G46350.1 47.297 74 38 1 185 258 184 256 7.59e-15 74.3
MsaG004942 AT2G37260.2 45.882 85 39 3 162 241 241 323 8.04e-15 74.3
MsaG004942 AT2G37260.2 41.935 93 52 2 178 270 77 167 2.03e-14 73.2
MsaG004942 AT5G49520.1 50.769 65 30 2 180 244 218 280 1.22e-14 74.3
MsaG004942 AT5G15130.1 45.714 70 38 0 185 254 228 297 1.38e-14 74.7
MsaG004942 AT5G43290.1 39.560 91 54 1 151 241 81 170 1.43e-14 72.8
MsaG004942 AT4G18170.1 51.613 62 29 1 185 246 173 233 1.44e-14 73.6
MsaG004942 AT5G15130.2 45.714 70 38 0 185 254 186 255 1.56e-14 74.3
MsaG004942 AT3G01080.3 42.308 78 43 2 180 257 201 276 5.82e-14 71.6
MsaG004942 AT4G23810.1 35.115 131 65 5 185 310 159 274 6.98e-14 71.6
MsaG004942 AT4G26640.7 43.478 69 38 1 181 249 338 405 6.99e-14 72.4
MsaG004942 AT4G26640.2 43.478 69 38 1 181 249 378 445 7.46e-14 72.4
MsaG004942 AT4G26640.1 43.478 69 38 1 181 249 306 373 8.70e-14 72.0
MsaG004942 AT1G69810.1 47.887 71 36 1 185 254 204 274 9.43e-14 71.6
MsaG004942 AT4G26640.5 43.478 69 38 1 181 249 202 269 9.64e-14 71.6
MsaG004942 AT4G26640.6 43.478 69 38 1 181 249 202 269 9.64e-14 71.6
MsaG004942 AT4G26640.4 43.478 69 38 1 181 249 202 269 9.64e-14 71.6
MsaG004942 AT4G26640.3 43.478 69 38 1 181 249 202 269 9.64e-14 71.6
MsaG004942 AT1G80840.1 39.437 71 43 0 185 255 147 217 1.14e-13 70.9
MsaG004942 AT2G30250.1 44.000 75 40 2 172 245 315 388 1.15e-13 71.2
MsaG004942 AT1G69810.2 47.887 71 36 1 185 254 146 216 1.25e-13 70.9
MsaG004942 AT2G25000.2 46.667 60 32 0 185 244 104 163 2.10e-13 68.9
MsaG004942 AT2G25000.4 46.667 60 32 0 185 244 104 163 2.10e-13 68.9
MsaG004942 AT2G25000.3 46.667 60 32 0 185 244 126 185 3.30e-13 68.6
MsaG004942 AT2G44745.1 41.026 78 44 2 165 241 125 201 3.65e-13 68.2
MsaG004942 AT1G18860.1 50.000 60 30 0 185 244 240 299 3.84e-13 70.1
MsaG004942 AT4G31800.2 41.558 77 45 0 185 261 112 188 3.87e-13 68.6
MsaG004942 AT2G40750.1 34.426 122 65 5 183 290 151 271 4.72e-13 69.3
MsaG004942 AT5G64810.1 49.123 57 28 1 185 241 111 166 4.89e-13 67.0
MsaG004942 AT1G55600.1 37.500 80 48 2 166 244 288 366 4.99e-13 69.7
MsaG004942 AT2G25000.1 46.667 60 32 0 185 244 147 206 5.19e-13 68.6
MsaG004942 AT4G31800.1 41.558 77 45 0 185 261 177 253 5.86e-13 68.6
MsaG004942 AT2G46130.1 40.000 80 46 2 163 241 8 86 6.88e-13 64.7
MsaG004942 AT5G41570.1 39.744 78 45 2 165 241 78 154 8.28e-13 66.2
MsaG004942 AT1G64000.1 45.161 62 33 1 180 241 110 170 8.62e-13 66.6
MsaG004942 AT2G46400.1 33.333 117 72 3 129 239 45 161 8.83e-13 68.2
MsaG004942 AT2G47260.1 37.864 103 54 4 166 260 154 254 1.03e-12 68.2
MsaG004942 AT3G56400.1 41.026 78 39 3 164 239 105 177 1.38e-12 67.4
MsaG004942 AT3G01970.1 43.548 62 34 1 181 242 62 122 2.27e-12 64.3
MsaG004942 AT5G13080.1 42.623 61 34 1 181 241 64 123 2.43e-12 63.9
MsaG004942 AT4G11070.2 50.877 57 26 1 185 239 110 166 3.81e-12 65.9
MsaG004942 AT4G11070.1 50.877 57 26 1 185 239 142 198 3.90e-12 66.2
MsaG004942 AT5G24110.1 39.024 82 48 1 160 239 89 170 5.05e-12 65.9
MsaG004942 AT4G39410.1 42.667 75 42 1 185 259 224 297 1.04e-11 65.1

Find 78 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATGTGGAGAACCTTTAA+TGG 0.267330 1:+75988796 None:intergenic
TGCAATATGAGGAGGGGAAT+TGG 0.276980 1:+75989326 None:intergenic
TCTTGAAGCTCATTGGTTAT+AGG 0.277524 1:+75989417 None:intergenic
GTGTTTGAGGCTGGATGCTT+TGG 0.300180 1:+75989133 None:intergenic
CTTTCGCGCGGTACAACCTT+TGG 0.306785 1:+75988555 None:intergenic
TTGTGGGCATGGCGAAAATA+TGG 0.311492 1:-75988827 MsaT004942.1:CDS
TGTTTGAGGCTGGATGCTTT+GGG 0.315208 1:+75989134 None:intergenic
GTTATAGGTTGCACTACATT+AGG 0.317458 1:+75989432 None:intergenic
TCTTTAGCACCAAACTCTTT+TGG 0.322210 1:-75988352 MsaT004942.1:CDS
GCAATATGAGGAGGGGAATT+GGG 0.331773 1:+75989327 None:intergenic
TCTTCTTCACCAAAAGAGTT+TGG 0.338560 1:+75988343 None:intergenic
CTACCACTTCCTCTTCCATT+TGG 0.346152 1:-75989269 MsaT004942.1:CDS
AAACACCTAGATCAAGAAAA+AGG 0.350761 1:-75989116 MsaT004942.1:intron
GGGTAAAAGATGTCAATAAT+TGG 0.354816 1:+75989388 None:intergenic
AGGAAGTGGTAGGGACAAAA+TGG 0.358832 1:+75989280 None:intergenic
TATAACAATGGCTGAGGATT+GGG 0.363678 1:-75989598 None:intergenic
TCTGTTGTTGTTGTAATTGT+TGG 0.364018 1:+75989196 None:intergenic
TTGATCTAGGTGTTTGAGGC+TGG 0.368009 1:+75989124 None:intergenic
TGATTGGCAGCTTCGAACTA+TGG 0.377700 1:+75989566 None:intergenic
TTTCTTGATCTAGGTGTTTG+AGG 0.387303 1:+75989120 None:intergenic
GTTGTTGTAATTGTTGGTTA+TGG 0.398557 1:+75989202 None:intergenic
GTTTAGATCTTGAAGCTCAT+TGG 0.407230 1:+75989410 None:intergenic
GGATGTGGAGAACCTTTAAT+GGG 0.413222 1:+75988797 None:intergenic
TTCGCAATTAGAAGAGGAAA+TGG 0.415743 1:+75989485 None:intergenic
TTCTGTTGTGGATGTATTGG+TGG 0.430239 1:+75989542 None:intergenic
GAAGTTTCTTGCACAATATG+AGG 0.432485 1:+75989246 None:intergenic
TGGATGTATTGGTGGCTGAT+TGG 0.440667 1:+75989550 None:intergenic
ATGTTCACTGTGACATATAC+AGG 0.458166 1:-75988502 MsaT004942.1:CDS
TTGCACAATATGAGGCCAAA+TGG 0.465159 1:+75989254 None:intergenic
TTATAACAATGGCTGAGGAT+TGG 0.466049 1:-75989599 None:intergenic
CAAGAAGAAGTTGAGCTGTT+GGG 0.466337 1:+75988379 None:intergenic
CGGATGTGTCACCGGAGACT+TGG 0.472713 1:+75988423 None:intergenic
AGACTTGGTTCGTGTACTTC+CGG 0.473267 1:+75988438 None:intergenic
GCAAGAAGAAGTTGAGCTGT+TGG 0.473676 1:+75988378 None:intergenic
TGCATTTATAACAATGGCTG+AGG 0.489485 1:-75989604 None:intergenic
AGAGGTTATCTGCGGTTACA+TGG 0.495635 1:+75988858 None:intergenic
TAACCTCTCAACAGATTTGT+GGG 0.500103 1:-75988843 MsaT004942.1:CDS
AAAAGAGTTTGGTGCTAAAG+AGG 0.510944 1:+75988354 None:intergenic
AGGCCAAATGGAAGAGGAAG+TGG 0.511721 1:+75989266 None:intergenic
TTCCGGCTAGCGAGTTCCGA+TGG 0.517215 1:+75988455 None:intergenic
TTCTACTTCGCAATTAGAAG+AGG 0.517896 1:+75989479 None:intergenic
TCCCTTGGTCATGTTTAGGA+AGG 0.527622 1:+75988262 None:intergenic
TTATATCTGTACCTTGGATG+TGG 0.528855 1:+75988782 None:intergenic
ATAACCTCTCAACAGATTTG+TGG 0.535451 1:-75988844 MsaT004942.1:CDS
CTAGTAGTAGTGGTGATGGT+GGG 0.535550 1:+75989366 None:intergenic
ACCCATCGGAACTCGCTAGC+CGG 0.537516 1:-75988457 MsaT004942.1:CDS
TCCGATGGGTAGGTCTTGCA+TGG 0.545684 1:+75988470 None:intergenic
CCTCATATTGCAAAATACAT+TGG 0.547832 1:-75989315 MsaT004942.1:CDS
GAGGTGGTCGGATGTGTCAC+CGG 0.563562 1:+75988415 None:intergenic
TAGTAGTAGTGGTGATGGTG+GGG 0.565045 1:+75989367 None:intergenic
TTAGAAGAGGAAATGGAAGA+TGG 0.565399 1:+75989492 None:intergenic
GTTTGAGGCTGGATGCTTTG+GGG 0.566369 1:+75989135 None:intergenic
AATATGAGGCCAAATGGAAG+AGG 0.570788 1:+75989260 None:intergenic
TGACTAGAAATTGAGGTGGT+CGG 0.577828 1:+75988403 None:intergenic
GAATATGTTGAAGCTAGAAG+TGG 0.578885 1:+75989224 None:intergenic
TCCGGCTAGCGAGTTCCGAT+GGG 0.583330 1:+75988456 None:intergenic
AGTAGTAGTGGTGATGGTGG+GGG 0.585398 1:+75989368 None:intergenic
TCTCAACAGATTTGTGGGCA+TGG 0.588913 1:-75988838 MsaT004942.1:CDS
GAAATTGAAACTGATGATGA+TGG 0.590055 1:-75988304 MsaT004942.1:CDS
GTTGGGTTGACTAGAAATTG+AGG 0.607818 1:+75988396 None:intergenic
AAGTACACGAACCAAGTCTC+CGG 0.613947 1:-75988434 MsaT004942.1:CDS
TTAAAGGTTCTCCACATCCA+AGG 0.614226 1:-75988793 MsaT004942.1:intron
AAATGGAAGAGGAAGTGGTA+GGG 0.618377 1:+75989271 None:intergenic
ACTATGACCACTAGTAGTAG+TGG 0.620104 1:+75989356 None:intergenic
TATAGATGTAGCAGTTCCAA+AGG 0.623205 1:-75988571 MsaT004942.1:CDS
CAAATGGAAGAGGAAGTGGT+AGG 0.623737 1:+75989270 None:intergenic
ACTAGTAGTAGTGGTGATGG+TGG 0.627374 1:+75989365 None:intergenic
CCTTTCAACTTGCTTTCGCG+CGG 0.627775 1:+75988543 None:intergenic
ATCTGTTGAGAGGTTATCTG+CGG 0.627787 1:+75988850 None:intergenic
GCTAGCGAGTTCCGATGGGT+AGG 0.628478 1:+75988460 None:intergenic
CCGCGCGAAAGCAAGTTGAA+AGG 0.629161 1:-75988543 MsaT004942.1:CDS
AGAAGAAGAGGACATCGACA+TGG 0.634163 1:-75988326 MsaT004942.1:CDS
ACCATCACCACTACTACTAG+TGG 0.637150 1:-75989363 MsaT004942.1:CDS
ACCACTAGTAGTAGTGGTGA+TGG 0.637486 1:+75989362 None:intergenic
ATGCCCACAAATCTGTTGAG+AGG 0.641173 1:+75988840 None:intergenic
AGATGGTATAGTATTTGTCG+TGG 0.665737 1:+75989509 None:intergenic
ACCATGCAAGACCTACCCAT+CGG 0.674071 1:-75988471 MsaT004942.1:CDS
GGGTTGACTAGAAATTGAGG+TGG 0.732424 1:+75988399 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 75988276 75989613 75988276 ID=MsaG004942
Chr1 mRNA 75988276 75989613 75988276 ID=MsaT004942.1;Parent=MsaG004942
Chr1 exon 75988276 75988600 75988276 ID=MsaT004942.1.exon3;Parent=MsaT004942.1
Chr1 CDS 75988276 75988600 75988276 ID=cds.MsaT004942.1;Parent=MsaT004942.1
Chr1 exon 75988794 75988907 75988794 ID=MsaT004942.1.exon2;Parent=MsaT004942.1
Chr1 CDS 75988794 75988907 75988794 ID=cds.MsaT004942.1;Parent=MsaT004942.1
Chr1 exon 75989117 75989613 75989117 ID=MsaT004942.1.exon1;Parent=MsaT004942.1
Chr1 CDS 75989117 75989613 75989117 ID=cds.MsaT004942.1;Parent=MsaT004942.1
Gene Sequence

>MsaT004942.1

ATGGCTGAGGATTGGGATTTGTTTGCCATAGTTCGAAGCTGCCAATCAGCCACCAATACATCCACAACAGAAAATTCATTTACCACGACAAATACTATACCATCTTCCATTTCCTCTTCTAATTGCGAAGTAGAATTAGAATATGATGCATTTTCTTTTCCTAATGTAGTGCAACCTATAACCAATGAGCTTCAAGATCTAAACCAATTATTGACATCTTTTACCCCCACCATCACCACTACTACTAGTGGTCATAGTATCAATCCCAATTCCCCTCCTCATATTGCAAAATACATTGGACAACAACAAAACCATTTTGTCCCTACCACTTCCTCTTCCATTTGGCCTCATATTGTGCAAGAAACTTCCACTTCTAGCTTCAACATATTCCATAACCAACAATTACAACAACAACAGATTCAACTACAAGCAATTCAGAAACAAGAATTTCAAGTACCCCAAAGCATCCAGCCTCAAACACCTAGATCAAGAAAAAGAAAAAGTCAACAAGGGAAAATGGTGTGCCATGTAACCGCAGATAACCTCTCAACAGATTTGTGGGCATGGCGAAAATATGGACAAAAACCCATTAAAGGTTCTCCACATCCAAGGAACTACTATAGATGTAGCAGTTCCAAAGGTTGTACCGCGCGAAAGCAAGTTGAAAGGAGCAACACTGAAGCAGACATGTTCACTGTGACATATACAGGAGATCATAACCATGCAAGACCTACCCATCGGAACTCGCTAGCCGGAAGTACACGAACCAAGTCTCCGGTGACACATCCGACCACCTCAATTTCTAGTCAACCCAACAGCTCAACTTCTTCTTGCTCCTCTTTAGCACCAAACTCTTTTGGTGAAGAAGAAGAGGACATCGACATGGAAATTGAAACTGATGATGATGGTGAAGATAGTTGTGATATTCCTTCCTAA
Protein sequence

>MsaT004942.1

MAEDWDLFAIVRSCQSATNTSTTENSFTTTNTIPSSISSSNCEVELEYDAFSFPNVVQPITNELQDLNQLLTSFTPTITTTTSGHSINPNSPPHIAKYIGQQQNHFVPTTSSSIWPHIVQETSTSSFNIFHNQQLQQQQIQLQAIQKQEFQVPQSIQPQTPRSRKRKSQQGKMVCHVTADNLSTDLWAWRKYGQKPIKGSPHPRNYYRCSSSKGCTARKQVERSNTEADMFTVTYTGDHNHARPTHRNSLAGSTRTKSPVTHPTTSISSQPNSSTSSCSSLAPNSFGEEEEDIDMEIETDDDGEDSCDIPS*