AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG004999

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004999 MtrunA17_Chr1g0203081 96.016 979 27 1 1 967 1 979 0.0 1938
MsaG004999 MtrunA17_Chr6g0472281 69.394 709 182 9 1 691 1 692 0.0 975
MsaG004999 MtrunA17_Chr6g0472281 51.364 220 81 4 759 967 653 857 1.28e-50 192
MsaG004999 MtrunA17_Chr3g0143921 42.730 564 281 12 124 667 139 680 1.40e-136 432
MsaG004999 MtrunA17_Chr3g0143921 45.946 74 39 1 874 946 818 891 2.16e-12 71.2
MsaG004999 MtrunA17_Chr2g0329671 39.303 603 318 15 113 696 167 740 2.03e-117 383
MsaG004999 MtrunA17_Chr2g0329671 45.745 94 50 1 872 965 865 957 1.86e-17 87.8
MsaG004999 MtrunA17_Chr4g0033791 40.038 527 292 9 124 645 147 654 2.59e-113 370
MsaG004999 MtrunA17_Chr4g0033791 45.122 82 44 1 873 954 826 906 1.63e-15 81.6
MsaG004999 MtrunA17_Chr5g0448621 35.135 370 180 9 124 478 126 450 1.17e-55 209
MsaG004999 MtrunA17_Chr5g0448621 65.657 99 27 1 564 662 607 698 1.15e-31 133
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG004999 AT4G24020.1 53.947 988 334 23 36 967 36 958 0.0 932
MsaG004999 AT1G64530.1 59.915 706 244 15 29 704 5 701 0.0 815
MsaG004999 AT1G64530.1 57.426 101 40 2 868 967 738 836 7.21e-28 121
MsaG004999 AT1G64530.2 62.271 546 182 9 179 704 5 546 0.0 674
MsaG004999 AT1G64530.2 58.586 99 38 2 870 967 585 681 6.45e-28 121
MsaG004999 AT4G24020.2 52.624 743 254 19 258 967 1 678 0.0 669
MsaG004999 AT1G76350.2 43.894 565 277 12 120 670 103 641 6.14e-138 433
MsaG004999 AT1G76350.2 35.238 105 51 2 843 946 698 786 2.83e-12 71.2
MsaG004999 AT1G76350.1 43.894 565 277 12 120 670 103 641 6.14e-138 433
MsaG004999 AT1G76350.1 35.238 105 51 2 843 946 698 786 2.83e-12 71.2
MsaG004999 AT1G76350.4 43.894 565 277 12 120 670 103 641 6.14e-138 433
MsaG004999 AT1G76350.4 35.238 105 51 2 843 946 698 786 2.83e-12 71.2
MsaG004999 AT1G76350.3 43.894 565 277 12 120 670 103 641 6.14e-138 433
MsaG004999 AT1G76350.3 35.238 105 51 2 843 946 698 786 2.83e-12 71.2
MsaG004999 AT1G20640.1 42.124 565 299 8 120 668 101 653 2.26e-129 412
MsaG004999 AT1G20640.1 39.024 82 48 2 872 952 745 825 6.92e-11 67.0
MsaG004999 AT1G20640.3 42.124 565 299 8 120 668 101 653 2.26e-129 412
MsaG004999 AT1G20640.3 39.024 82 48 2 872 952 745 825 6.92e-11 67.0
MsaG004999 AT1G20640.2 42.124 565 299 8 120 668 101 653 2.26e-129 412
MsaG004999 AT1G20640.2 39.024 82 48 2 872 952 745 825 6.92e-11 67.0
MsaG004999 AT2G17150.1 39.632 598 293 15 124 673 124 701 1.62e-122 395
MsaG004999 AT2G17150.1 41.860 86 46 2 855 939 798 880 1.52e-13 75.5
MsaG004999 AT2G17150.6 39.632 598 293 15 124 673 124 701 1.62e-122 395
MsaG004999 AT2G17150.6 41.860 86 46 2 855 939 798 880 1.52e-13 75.5
MsaG004999 AT3G59580.2 39.360 531 288 9 124 647 106 609 9.42e-115 374
MsaG004999 AT3G59580.2 42.857 98 53 3 871 966 792 888 5.61e-16 83.6
MsaG004999 AT3G59580.3 39.360 531 288 9 124 647 106 609 9.42e-115 374
MsaG004999 AT3G59580.3 42.857 98 53 3 871 966 792 888 5.61e-16 83.6
MsaG004999 AT3G59580.1 39.360 531 288 9 124 647 106 609 9.42e-115 374
MsaG004999 AT3G59580.1 42.857 98 53 3 871 966 792 888 5.61e-16 83.6
MsaG004999 AT2G43500.3 36.498 611 349 10 47 646 81 663 1.99e-114 374
MsaG004999 AT2G43500.3 43.750 96 53 1 871 966 834 928 7.17e-19 92.8
MsaG004999 AT2G43500.9 36.498 611 349 10 47 646 81 663 1.99e-114 374
MsaG004999 AT2G43500.9 43.750 96 53 1 871 966 834 928 7.17e-19 92.8
MsaG004999 AT2G43500.6 36.498 611 349 10 47 646 81 663 1.99e-114 374
MsaG004999 AT2G43500.6 43.750 96 53 1 871 966 834 928 7.17e-19 92.8
MsaG004999 AT2G43500.1 36.498 611 349 10 47 646 81 663 1.99e-114 374
MsaG004999 AT2G43500.1 43.750 96 53 1 871 966 834 928 7.17e-19 92.8
MsaG004999 AT2G43500.10 36.498 611 349 10 47 646 81 663 1.99e-114 374
MsaG004999 AT2G43500.10 43.750 96 53 1 871 966 834 928 7.17e-19 92.8
MsaG004999 AT2G43500.11 36.498 611 349 10 47 646 81 663 1.99e-114 374
MsaG004999 AT2G43500.11 43.750 96 53 1 871 966 834 928 7.17e-19 92.8
MsaG004999 AT2G43500.7 36.498 611 349 10 47 646 81 663 1.99e-114 374
MsaG004999 AT2G43500.7 43.750 96 53 1 871 966 834 928 7.17e-19 92.8
MsaG004999 AT2G43500.8 36.498 611 349 10 47 646 81 663 1.99e-114 374
MsaG004999 AT2G43500.8 43.750 96 53 1 871 966 834 928 7.17e-19 92.8
MsaG004999 AT2G43500.5 35.897 624 348 11 47 646 81 676 2.09e-111 366
MsaG004999 AT2G43500.5 43.750 96 53 1 871 966 847 941 6.59e-19 93.2
MsaG004999 AT2G43500.4 35.897 624 348 11 47 646 81 676 2.09e-111 366
MsaG004999 AT2G43500.4 43.750 96 53 1 871 966 847 941 6.59e-19 93.2
MsaG004999 AT2G43500.2 35.577 624 350 11 47 646 81 676 5.40e-110 363
MsaG004999 AT2G43500.2 43.750 96 53 1 871 966 847 941 6.70e-19 93.2
MsaG004999 AT4G38340.2 35.985 528 265 12 124 647 43 501 6.97e-94 313
MsaG004999 AT4G38340.2 37.755 98 55 2 858 952 592 686 1.11e-14 79.0
MsaG004999 AT4G38340.1 35.985 528 265 12 124 647 114 572 7.68e-93 311
MsaG004999 AT4G38340.1 37.755 98 55 2 858 952 663 757 1.31e-14 79.0
MsaG004999 AT2G17150.2 36.557 517 270 13 124 595 124 627 1.11e-92 308
MsaG004999 AT2G17150.5 36.731 520 268 14 124 595 124 630 3.89e-92 307
MsaG004999 AT2G17150.3 36.731 520 268 14 124 595 124 630 3.89e-92 307
MsaG004999 AT4G35270.2 40.710 366 203 4 124 485 153 508 1.11e-84 293
MsaG004999 AT4G35270.2 60.345 116 39 1 560 675 635 743 4.45e-35 145
MsaG004999 AT4G35270.2 42.105 95 52 3 874 966 865 958 4.67e-15 80.5
MsaG004999 AT4G35270.1 40.710 366 203 4 124 485 153 508 1.11e-84 293
MsaG004999 AT4G35270.1 60.345 116 39 1 560 675 635 743 4.45e-35 145
MsaG004999 AT4G35270.1 42.105 95 52 3 874 966 865 958 4.67e-15 80.5
MsaG004999 AT4G35270.3 40.710 366 203 4 124 485 153 508 1.11e-84 293
MsaG004999 AT4G35270.3 60.345 116 39 1 560 675 635 743 4.45e-35 145
MsaG004999 AT4G35270.3 42.105 95 52 3 874 966 865 958 4.67e-15 80.5
MsaG004999 AT4G35270.4 40.710 366 203 4 124 485 153 508 1.11e-84 293
MsaG004999 AT4G35270.4 60.345 116 39 1 560 675 635 743 4.45e-35 145
MsaG004999 AT4G35270.4 42.105 95 52 3 874 966 865 958 4.67e-15 80.5
MsaG004999 AT2G17150.4 36.308 493 238 15 257 702 3 466 2.65e-76 263
MsaG004999 AT2G17150.4 41.860 86 46 2 855 939 543 625 1.28e-13 75.5

Find 225 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCCACCAATAGAAAATT+TGG 0.126044 1:-76534908 MsaT004999.1:CDS
AACCTCAAGTTTCAATCTTT+TGG 0.135797 1:+76531700 None:intergenic
ATATCGTAGATGCTCATTTA+TGG 0.198226 1:-76533679 MsaT004999.1:CDS
GTTCATTATGCTCTCATGTT+TGG 0.230421 1:-76533527 MsaT004999.1:CDS
TTCAGTAGCTGAGGCCATTT+TGG 0.234654 1:-76531939 MsaT004999.1:intron
GAGAAGATATCATAAGGTTT+AGG 0.254091 1:-76531766 MsaT004999.1:CDS
ACCCGATGCCTCACCAAATA+TGG 0.272048 1:-76533219 MsaT004999.1:CDS
CTGTTTCATTGCTGACAATA+TGG 0.286199 1:+76533382 None:intergenic
CTTGAGAAAGAAGTGATTAA+TGG 0.295335 1:-76532300 MsaT004999.1:CDS
TTCATTATGCTCTCATGTTT+GGG 0.297926 1:-76533526 MsaT004999.1:CDS
CTGTAGGCATAGGAGTGTTT+TGG 0.304108 1:-76533792 MsaT004999.1:CDS
AGAAGAACATGGTTGTTCTA+TGG 0.308360 1:-76535844 MsaT004999.1:CDS
AATGAAGACTGTAACTATTA+AGG 0.320892 1:-76531798 MsaT004999.1:CDS
TGGTAAAGGCGTGACTATTC+AGG 0.321308 1:-76532280 MsaT004999.1:CDS
AAGTTGTTACCACAAGCTTC+TGG 0.322668 1:+76531905 None:intergenic
TATCGTAGATGCTCATTTAT+GGG 0.324285 1:-76533678 MsaT004999.1:CDS
GAATGTATTGGCTCAGGTTT+GGG 0.327805 1:-76534818 MsaT004999.1:CDS
CATTGCTGGCTGGACTTATT+CGG 0.328442 1:+76532447 None:intergenic
GGAAGCTCCTGTGAGAGTTC+AGG 0.336945 1:-76531527 MsaT004999.1:CDS
ATGATATCAATGCATTGTTT+AGG 0.337373 1:+76531857 None:intergenic
GCCTACAGGGAACCCATGTT+TGG 0.338019 1:+76533807 None:intergenic
AAGCAACATAATCCGGGTTT+TGG 0.339808 1:-76531573 MsaT004999.1:CDS
ATCAACCTTCTTCTCCTCTA+TGG 0.342830 1:-76535728 MsaT004999.1:CDS
GATTTAATAGATTGAGCAAT+AGG 0.344298 1:+76533251 None:intergenic
GTTTGTTTCTGATACTTTAG+AGG 0.346300 1:+76532371 None:intergenic
CACAACATGTTGTTCATTGT+TGG 0.348163 1:+76532122 None:intergenic
AGCATGTGATGCAGACCTTC+AGG 0.350853 1:-76531627 MsaT004999.1:CDS
GGCGTGACTATTCAGGAAAT+TGG 0.356009 1:-76532273 MsaT004999.1:CDS
TTTATGGTTGGAATTTCAAT+TGG 0.358415 1:+76532162 None:intergenic
AAATTGGAAAGGACCGAAAA+AGG 0.359121 1:-76532257 MsaT004999.1:CDS
GCTTGTGGTAACAACTTTCC+AGG 0.361722 1:-76531899 MsaT004999.1:CDS
AGAAGATATCATAAGGTTTA+GGG 0.363466 1:-76531765 MsaT004999.1:CDS
TATTGCTTAACGAGCATTGC+TGG 0.371298 1:+76532433 None:intergenic
CAGATACGCTTCATTGTAGT+AGG 0.378516 1:+76532645 None:intergenic
TTTAGAAACACCATGGCCTT+TGG 0.382797 1:-76535808 MsaT004999.1:CDS
TGAATGTATTGGCTCAGGTT+TGG 0.382919 1:-76534819 MsaT004999.1:CDS
TGGAGCCAGATCCTCCAAAA+TGG 0.387883 1:+76531925 None:intergenic
TAACACGTTTGAGCTTAGAT+AGG 0.389963 1:+76532565 None:intergenic
CTCCATATTTGGTGAGGCAT+CGG 0.392508 1:+76533217 None:intergenic
CGGTATGTACTTACAACTTC+AGG 0.393209 1:-76534775 MsaT004999.1:CDS
CAACATGGTCAAGGTGTTGC+GGG 0.395005 1:-76533623 MsaT004999.1:CDS
CCGGAGGAAGCAACATAATC+CGG 0.406793 1:-76531580 MsaT004999.1:CDS
TTATGGTTGGAATTTCAATT+GGG 0.410388 1:+76532163 None:intergenic
TTAGAATAGTACAGAACATT+GGG 0.411506 1:+76534604 None:intergenic
CTGGACTTATTCGGAGTAGA+AGG 0.413845 1:+76532456 None:intergenic
CTTTCCAGGTTCATCACATC+TGG 0.413983 1:-76531885 MsaT004999.1:CDS
GATGGAACTCTAGGACTTCC+TGG 0.418289 1:-76534661 MsaT004999.1:CDS
AAATATGGAGGAGGAGGAGG+AGG 0.419910 1:-76533204 MsaT004999.1:CDS
TGGTCAAGGTGTTGCGGGGA+GGG 0.420611 1:-76533618 MsaT004999.1:CDS
TGACATGGGATTGGTGATAA+AGG 0.425035 1:+76535776 None:intergenic
CCGGATTATGTTGCTTCCTC+CGG 0.426310 1:+76531580 None:intergenic
TAAATGAGCATCTACGATAT+AGG 0.426423 1:+76533682 None:intergenic
TTTGGTGAGGCATCGGGTCT+AGG 0.427870 1:+76533224 None:intergenic
GTTGATCCGATGTCTACCTT+AGG 0.428534 1:-76533131 MsaT004999.1:CDS
CCTTGCAGGCAGTAAATTTG+AGG 0.428965 1:-76534239 MsaT004999.1:intron
TCCATATTTGGTGAGGCATC+GGG 0.432185 1:+76533218 None:intergenic
GAAGCTCCTGTGAGAGTTCA+GGG 0.444633 1:-76531526 MsaT004999.1:CDS
CATGAACAGAAGACGCTGTT+GGG 0.447455 1:-76533407 MsaT004999.1:CDS
GGATGGATATTGTGTAATCA+AGG 0.447561 1:-76534887 MsaT004999.1:CDS
TCAGGAAATGAACTAACAAT+TGG 0.451708 1:+76532480 None:intergenic
CCACAGAAAACATGTACATC+AGG 0.453576 1:+76534695 None:intergenic
CAGAGTCTTAACATTGCTGC+TGG 0.455084 1:-76533353 MsaT004999.1:CDS
TTGTTGTCGTCTTTCTCCAC+CGG 0.456133 1:+76534949 None:intergenic
AAGTTCTGCTATCACATTGT+CGG 0.456387 1:+76532029 None:intergenic
AGGGCGTCAAAATGCATTAT+CGG 0.458489 1:-76533882 MsaT004999.1:CDS
ACCAAATATGGAGGAGGAGG+AGG 0.458759 1:-76533207 MsaT004999.1:CDS
TTGAGGGGATCGCCAGAGTC+GGG 0.458933 1:-76532099 MsaT004999.1:CDS
AGTTCTGCTATCACATTGTC+GGG 0.459703 1:+76532030 None:intergenic
CTGCAAGACAGATTACCCTT+TGG 0.463297 1:-76533549 MsaT004999.1:CDS
CACATTTGATATCAAGTACA+TGG 0.464870 1:-76531675 MsaT004999.1:CDS
GGAGGAGGAGGGAGTACCAC+AGG 0.466897 1:-76533192 MsaT004999.1:CDS
CATGACATAACATCAAATCT+TGG 0.469302 1:-76531548 MsaT004999.1:CDS
AGCAATGCTCGTTAAGCAAT+AGG 0.471004 1:-76532431 MsaT004999.1:CDS
GCGTTTGATTTGAATTCTCT+TGG 0.473199 1:-76532516 MsaT004999.1:CDS
GTCCTTGATCCGCATAGTAA+TGG 0.473816 1:-76534742 MsaT004999.1:CDS
CTCTTTGAACTGTGGCATTG+TGG 0.474442 1:-76531741 MsaT004999.1:CDS
TTAAATCAGATTTGCAATGA+AGG 0.476823 1:-76533902 MsaT004999.1:intron
CACCTGTGAGGAATGGTAAT+CGG 0.477678 1:-76534795 MsaT004999.1:CDS
CAAACGCTCCTTCAGCGCCT+TGG 0.478043 1:+76532532 None:intergenic
ATTATTGAAGCAACTGATGA+AGG 0.480520 1:-76533299 MsaT004999.1:CDS
CCTTCGTTGCAGTCATGTAT+TGG 0.482068 1:-76534508 MsaT004999.1:CDS
GGAGAGAACGATGGAACTCT+AGG 0.486383 1:-76534670 MsaT004999.1:CDS
GTAATCTGTCTTGCAGAACT+TGG 0.486621 1:+76533556 None:intergenic
GGTGTTGAACTCGAGGAAAA+TGG 0.487027 1:-76533332 MsaT004999.1:CDS
AGAACATTGGGAGACCATTC+TGG 0.493005 1:+76534616 None:intergenic
TAAATCAGATTTGCAATGAA+GGG 0.493978 1:-76533901 MsaT004999.1:intron
TTCAGCAGAAACTACCAGAA+TGG 0.494743 1:-76534630 MsaT004999.1:CDS
ATTAGAATAGTACAGAACAT+TGG 0.496307 1:+76534603 None:intergenic
CCTACAGGGAACCCATGTTT+GGG 0.498512 1:+76533808 None:intergenic
TGTAATGTTGAGCATGATCT+CGG 0.498840 1:+76534566 None:intergenic
TTTCCACGTTTCCTCTCTGA+GGG 0.500087 1:+76533089 None:intergenic
TGAACTGAATGTATTGGCTC+AGG 0.500170 1:-76534824 MsaT004999.1:CDS
ACATGGACGATGATAATGAA+TGG 0.500704 1:-76531658 MsaT004999.1:CDS
CATATAGAGAAGATATCATA+AGG 0.502450 1:-76531772 MsaT004999.1:CDS
CCACCAATAGAAAATTTGGA+TGG 0.502580 1:-76534904 MsaT004999.1:CDS
TTGAGCAATAGGAATAGATT+CGG 0.502794 1:+76533262 None:intergenic
AAAGTCCATAGAGGAGAAGA+AGG 0.508706 1:+76535723 None:intergenic
TGGCATGAACCATGAGAAGT+AGG 0.511685 1:+76532195 None:intergenic
AAAGAAGTGATTAATGGTAA+AGG 0.511717 1:-76532294 MsaT004999.1:CDS
TTGATCCGATGTCTACCTTA+GGG 0.513112 1:-76533130 MsaT004999.1:CDS
TTACAACGTTCGTGGAACCT+TGG 0.514560 1:-76534548 MsaT004999.1:CDS
TCTTTCATCTGAGGCTCTGA+CGG 0.515877 1:+76532408 None:intergenic
TGCAACGAAGGTTCAAATAC+AGG 0.517092 1:+76534520 None:intergenic
CAAAACACTCCTATGCCTAC+AGG 0.518113 1:+76533793 None:intergenic
CTATCACATTGTCGGGCATA+CGG 0.518485 1:+76532037 None:intergenic
GCTTAACGAGCATTGCTGGC+TGG 0.519778 1:+76532437 None:intergenic
TCGGGCATACGGTGAGAAGT+TGG 0.519816 1:+76532048 None:intergenic
ATTTACCTCTAGCCCAAACA+TGG 0.520347 1:-76533820 MsaT004999.1:CDS
GGAACCCATGTTTGGGCTAG+AGG 0.520691 1:+76533815 None:intergenic
AACAAACATACAGATTGTAA+TGG 0.521216 1:-76532355 MsaT004999.1:CDS
GATGCTGCAAAGAGCCTTGG+CGG 0.522654 1:-76533008 MsaT004999.1:intron
AATTCATTCTCTTTCATCTG+AGG 0.523018 1:+76532399 None:intergenic
CAGGATCCATCAGAGGTTCA+TGG 0.523226 1:-76533173 MsaT004999.1:CDS
ATGTATGTTTCAAGATCATC+CGG 0.526490 1:-76531599 MsaT004999.1:CDS
GCTCAACATTACAACGTTCG+TGG 0.526984 1:-76534556 MsaT004999.1:CDS
GTCCATTACTATGCGGATCA+AGG 0.527800 1:+76534740 None:intergenic
GTCGGCTGCATTCCCGACTC+TGG 0.528364 1:+76532087 None:intergenic
ATGTTGAGCATGATCTCGGC+GGG 0.529020 1:+76534570 None:intergenic
AAAGATGCTGCAAAGAGCCT+TGG 0.531341 1:-76533011 MsaT004999.1:CDS
CATGGACGATGATAATGAAT+GGG 0.531492 1:-76531657 MsaT004999.1:CDS
TCATGAACAGAAGACGCTGT+TGG 0.531663 1:-76533408 MsaT004999.1:CDS
CGCTTCGTTACTTCAAAGAG+TGG 0.532079 1:-76534849 MsaT004999.1:CDS
AAAAGATTGAAACTTGAGGT+TGG 0.535106 1:-76531698 MsaT004999.1:CDS
ATATCAATGCATTGTTTAGG+AGG 0.537877 1:+76531860 None:intergenic
GAATCTGTCCAAGGCGCTGA+AGG 0.539465 1:-76532540 MsaT004999.1:CDS
TTCCAAAAGATTGAAACTTG+AGG 0.540955 1:-76531702 MsaT004999.1:CDS
AGCGTATCTGCAGGCAGCAT+GGG 0.541320 1:-76532632 MsaT004999.1:CDS
CCAATACATGACTGCAACGA+AGG 0.542039 1:+76534508 None:intergenic
CATGGGTTCCCTGTAGGCAT+AGG 0.542933 1:-76533802 MsaT004999.1:CDS
GAGGTTCATGGTGAAAATGT+AGG 0.543377 1:-76533161 MsaT004999.1:CDS
CTCACCAAATATGGAGGAGG+AGG 0.544347 1:-76533210 MsaT004999.1:CDS
TAAAAGAAACTCAACCTCTA+GGG 0.545210 1:+76534417 None:intergenic
AACACGTTTGAGCTTAGATA+GGG 0.545296 1:+76532566 None:intergenic
ATCGTAGATGCTCATTTATG+GGG 0.545393 1:-76533677 MsaT004999.1:CDS
CTACAATGAAGCGTATCTGC+AGG 0.545803 1:-76532641 MsaT004999.1:CDS
GTGGACTGAACTGAATGTAT+TGG 0.548852 1:-76534830 MsaT004999.1:CDS
GGAGAGTTTAAAGCATTAGT+CGG 0.550784 1:+76532069 None:intergenic
ACAACATGGTCAAGGTGTTG+CGG 0.552963 1:-76533624 MsaT004999.1:CDS
GTAATGGAAAATCAATTGCT+AGG 0.555652 1:-76532339 MsaT004999.1:CDS
AAATTCAATAACTGAGAAGC+CGG 0.559695 1:-76534968 MsaT004999.1:CDS
AAGCGTATCTGCAGGCAGCA+TGG 0.561590 1:-76532633 MsaT004999.1:CDS
CTCCTCCTCCATATTTGGTG+AGG 0.561991 1:+76533211 None:intergenic
AGCACAAACCCTACTTCTCA+TGG 0.568994 1:-76532204 MsaT004999.1:CDS
TGAACAACATGTTGTGTTGA+GGG 0.569993 1:-76532115 MsaT004999.1:CDS
ACATGTTGTTCATTGTTGGA+TGG 0.570109 1:+76532126 None:intergenic
TTATTCGGAGTAGAAGGCTC+AGG 0.570221 1:+76532462 None:intergenic
GGAGCCAGATGTGATGAACC+TGG 0.570260 1:+76531881 None:intergenic
TCTATTGGTGGCAAAAGGGG+TGG 0.571210 1:+76534916 None:intergenic
ATGGTCAAGGTGTTGCGGGG+AGG 0.571632 1:-76533619 MsaT004999.1:CDS
CGGAGGAAGCAACATAATCC+GGG 0.572590 1:-76531579 MsaT004999.1:CDS
TTCAAATACAGGCAAAGCCA+AGG 0.574356 1:+76534531 None:intergenic
ATGAGAGCATAATGAACCAA+AGG 0.574665 1:+76533533 None:intergenic
CTATGCGGATCAAGGACAAA+GGG 0.575242 1:+76534748 None:intergenic
CCCAAACATGGGTTCCCTGT+AGG 0.575392 1:-76533808 MsaT004999.1:CDS
AAAATACCTGAGTGAATGGA+TGG 0.576234 1:+76534199 None:intergenic
TCACTCCCCTGAACTCTCAC+AGG 0.578779 1:+76531520 None:intergenic
ACCTTGTTAATCTTTCGAGA+TGG 0.579070 1:+76532597 None:intergenic
TTATGTCATGCACCAAAACC+CGG 0.579981 1:+76531561 None:intergenic
TGAGAGCATAATGAACCAAA+GGG 0.580200 1:+76533534 None:intergenic
GTTGAGGGGATCGCCAGAGT+CGG 0.581271 1:-76532100 MsaT004999.1:CDS
TTTGGACCATCCATTCACTC+AGG 0.581838 1:-76534205 MsaT004999.1:intron
GTGTGGAGGATTGTTAATAG+AGG 0.582497 1:-76531998 MsaT004999.1:CDS
CTCAGGTTTGGGCACCTGTG+AGG 0.583880 1:-76534807 MsaT004999.1:CDS
AAACATACATGCACTCCTGA+AGG 0.585860 1:+76531612 None:intergenic
TGCCTCACCAAATATGGAGG+AGG 0.587748 1:-76533213 MsaT004999.1:CDS
GTTTGGGCACCTGTGAGGAA+TGG 0.588324 1:-76534802 MsaT004999.1:CDS
GACATGTGATCCAAAGGCCA+TGG 0.591421 1:+76535798 None:intergenic
AAAACACTCCTATGCCTACA+GGG 0.592526 1:+76533794 None:intergenic
ACTTCTCATGGTTCATGCCA+CGG 0.596610 1:-76532192 MsaT004999.1:CDS
AAAATCAATTGCTAGGAGGA+AGG 0.596853 1:-76532332 MsaT004999.1:CDS
TATGGTTGGAATTTCAATTG+GGG 0.600606 1:+76532164 None:intergenic
TTATTCCCTAAGGTAGACAT+CGG 0.604395 1:+76533125 None:intergenic
TGCTGCTGGTGTTGAACTCG+AGG 0.610461 1:-76533339 MsaT004999.1:CDS
CGTGTTATTGAATCTGTCCA+AGG 0.611341 1:-76532549 MsaT004999.1:CDS
GTTTGAGCTTAGATAGGGAA+CGG 0.611978 1:+76532571 None:intergenic
GGCAGCATGGGATATCTCGT+TGG 0.612803 1:-76532620 MsaT004999.1:CDS
TTTAGGGTCTCTTTGAACTG+TGG 0.613295 1:-76531749 MsaT004999.1:CDS
TCATGAAATTCAGTAATCCC+AGG 0.614579 1:+76533425 None:intergenic
ATGGTTGGAATTTCAATTGG+GGG 0.615940 1:+76532165 None:intergenic
CACAGAAAACATGTACATCA+GGG 0.616371 1:+76534696 None:intergenic
ATAAAGGACATGTGATCCAA+AGG 0.617226 1:+76535792 None:intergenic
GACTATTCAGGAAATTGGAA+AGG 0.619669 1:-76532268 MsaT004999.1:CDS
ATCTAGATTTAGAAACACCA+TGG 0.622079 1:-76535815 MsaT004999.1:CDS
GAACTTCAAGAGCCGTGTGG+AGG 0.625574 1:-76532012 MsaT004999.1:CDS
CCATCAGTAAAAGTCCATAG+AGG 0.625976 1:+76535714 None:intergenic
AGAGTATGAACGTACCGCCA+AGG 0.629334 1:+76532994 None:intergenic
TACCGATTACCATTCCTCAC+AGG 0.629926 1:+76534793 None:intergenic
AAAGATTAACTATGCTCCTG+AGG 0.633444 1:-76534458 MsaT004999.1:CDS
TTCAATAACTGAGAAGCCGG+TGG 0.635214 1:-76534965 MsaT004999.1:CDS
GGCATGAACCATGAGAAGTA+GGG 0.635585 1:+76532196 None:intergenic
ACATACAAGAGAAGATCTTG+TGG 0.635928 1:+76535652 None:intergenic
AGATACGCTTCATTGTAGTA+GGG 0.641314 1:+76532646 None:intergenic
AGTGTGCATGTCTACAACTG+AGG 0.643299 1:-76533711 MsaT004999.1:CDS
ACTATGCGGATCAAGGACAA+AGG 0.647160 1:+76534747 None:intergenic
TCATTGTTGGATGGAATGAA+CGG 0.648544 1:+76532135 None:intergenic
CGATGCCTCACCAAATATGG+AGG 0.650921 1:-76533216 MsaT004999.1:CDS
AAAAGGAATAGAACTAGGAG+TGG 0.652567 1:-76532240 MsaT004999.1:CDS
TTTACCTCTAGCCCAAACAT+GGG 0.653751 1:-76533819 MsaT004999.1:CDS
ATGAACAACATGTTGTGTTG+AGG 0.654614 1:-76532116 MsaT004999.1:CDS
TGGACTCAATCAGTATAGAA+CGG 0.656345 1:-76534722 MsaT004999.1:CDS
GGTGAGGCATCGGGTCTAGG+TGG 0.659977 1:+76533227 None:intergenic
ATGGAAAATCAATTGCTAGG+AGG 0.664194 1:-76532336 MsaT004999.1:CDS
AAACCCTCAGAGAGGAAACG+TGG 0.667434 1:-76533092 MsaT004999.1:CDS
GTGCATGTCTACAACTGAGG+CGG 0.669875 1:-76533708 MsaT004999.1:CDS
AACATGGTCAAGGTGTTGCG+GGG 0.672343 1:-76533622 MsaT004999.1:CDS
GTCGAACATCACTTACAACA+TGG 0.677843 1:-76533638 MsaT004999.1:CDS
GATCTTAGAGATATTGACAG+TGG 0.683127 1:-76533858 MsaT004999.1:CDS
AAAACATCATTAGAAGAACA+TGG 0.686020 1:-76535855 MsaT004999.1:CDS
GCCATCTCGAAAGATTAACA+AGG 0.686233 1:-76532598 MsaT004999.1:CDS
GATCTGGCTCCAGAAGCTTG+TGG 0.687276 1:-76531914 MsaT004999.1:CDS
AATGTTGAGCATGATCTCGG+CGG 0.687474 1:+76534569 None:intergenic
TCTATTAACAATCCTCCACA+CGG 0.690425 1:+76532000 None:intergenic
CATCACTTACAACATGGTCA+AGG 0.693887 1:-76533632 MsaT004999.1:CDS
TCTGTGGATGGAGAGAACGA+TGG 0.695263 1:-76534679 MsaT004999.1:CDS
AGTACCACAGGATCCATCAG+AGG 0.699060 1:-76533180 MsaT004999.1:CDS
ACATAAAGAAACCCTCAGAG+AGG 0.705928 1:-76533100 MsaT004999.1:CDS
GCAGAACTTCAAGAGCCGTG+TGG 0.707309 1:-76532015 MsaT004999.1:CDS
TGAACCTCTGATGGATCCTG+TGG 0.708138 1:+76533176 None:intergenic
AAGCTCCTGTGAGAGTTCAG+GGG 0.712104 1:-76531525 MsaT004999.1:CDS
TGTTGAGCATGATCTCGGCG+GGG 0.718991 1:+76534571 None:intergenic
GGATTGTTAATAGAGGACGC+TGG 0.727096 1:-76531991 MsaT004999.1:CDS
TATGTTTCAAGATCATCCGG+AGG 0.731584 1:-76531596 MsaT004999.1:CDS
GAACAACATGTTGTGTTGAG+GGG 0.732713 1:-76532114 MsaT004999.1:CDS
TGATTGAGTCCATTACTATG+CGG 0.743496 1:+76534733 None:intergenic
TGAAATTCAGTAATCCCAGG+AGG 0.744471 1:+76533428 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 76531520 76535922 76531520 ID=MsaG004999
Chr1 mRNA 76531520 76535922 76531520 ID=MsaT004999.1;Parent=MsaG004999
Chr1 exon 76531520 76531953 76531520 ID=MsaT004999.1.exon6;Parent=MsaT004999.1
Chr1 CDS 76531520 76531953 76531520 ID=cds.MsaT004999.1;Parent=MsaT004999.1
Chr1 exon 76531989 76532672 76531989 ID=MsaT004999.1.exon5;Parent=MsaT004999.1
Chr1 CDS 76531989 76532672 76531989 ID=cds.MsaT004999.1;Parent=MsaT004999.1
Chr1 exon 76533009 76533915 76533009 ID=MsaT004999.1.exon4;Parent=MsaT004999.1
Chr1 CDS 76533009 76533915 76533009 ID=cds.MsaT004999.1;Parent=MsaT004999.1
Chr1 exon 76534206 76534253 76534206 ID=MsaT004999.1.exon3;Parent=MsaT004999.1
Chr1 CDS 76534206 76534253 76534206 ID=cds.MsaT004999.1;Parent=MsaT004999.1
Chr1 exon 76534432 76534997 76534432 ID=MsaT004999.1.exon2;Parent=MsaT004999.1
Chr1 CDS 76534432 76534997 76534432 ID=cds.MsaT004999.1;Parent=MsaT004999.1
Chr1 exon 76535658 76535922 76535658 ID=MsaT004999.1.exon1;Parent=MsaT004999.1
Chr1 CDS 76535658 76535922 76535658 ID=cds.MsaT004999.1;Parent=MsaT004999.1
Gene Sequence

>MsaT004999.1

ATGTCAGAACCTGAGGAAGAAAATCAAGACTTTCCTCCCAAGACAAAAACATCATTAGAAGAACATGGTTGTTCTATGGATTTTGATCTAGATTTAGAAACACCATGGCCTTTGGATCACATGTCCTTTATCACCAATCCCATGTCACCTTTTTTGTTTTCAAACATCTCTGATCAACCTTCTTCTCCTCTATGGACTTTTACTGATGGAGAAGATGGCAAACTTGCAGCTTCAGCTTTCTCTGATTGCCACAAGATCTTCTCTTATGATTCAAATTCAATAACTGAGAAGCCGGTGGAGAAAGACGACAACAAAACACTTTTGCCACCCCTTTTGCCACCAATAGAAAATTTGGATGGATATTGTGTAATCAAGGAAAAGATGACACAAGCGCTTCGTTACTTCAAAGAGTGGACTGAACTGAATGTATTGGCTCAGGTTTGGGCACCTGTGAGGAATGGTAATCGGTATGTACTTACAACTTCAGGTCAACCCTTTGTCCTTGATCCGCATAGTAATGGACTCAATCAGTATAGAACGGTTTCCCTGATGTACATGTTTTCTGTGGATGGAGAGAACGATGGAACTCTAGGACTTCCTGGTCGAGTTTTTCAGCAGAAACTACCAGAATGGTCTCCCAATGTTCTGTACTATTCTAATAAAGAGTATCCCCGCCGAGATCATGCTCAACATTACAACGTTCGTGGAACCTTGGCTTTGCCTGTATTTGAACCTTCGTTGCAGTCATGTATTGGTGTGATAGAGTTGATAATGACTTCACTAAAGATTAACTATGCTCCTGAGGTTGAAAAAATCTGCAAAGCCCTAGAGGCAGTAAATTTGAGGAGCTCAGAGTTTTTGGACCATCCATTCACTCAGATTTGCAATGAAGGGCGTCAAAATGCATTATCGGAGATCTTAGAGATATTGACAGTGGTGTGTGAAACTCATAATTTACCTCTAGCCCAAACATGGGTTCCCTGTAGGCATAGGAGTGTTTTGGCTCATGGTGGCGGTTTCAAGAAAAGTTGTTCGAGTTTTGATGGTAGTTGCATGGGGCAAGTGTGCATGTCTACAACTGAGGCGGCAGCCTATATCGTAGATGCTCATTTATGGGGTTTCAGAGAAGCATGTGTCGAACATCACTTACAACATGGTCAAGGTGTTGCGGGGAGGGCGTTTTTGTCTCAAAACATGTGCTTCTGCACAAACATTACCAAGTTCTGCAAGACAGATTACCCTTTGGTTCATTATGCTCTCATGTTTGGGTTAACAAGCTGTTTTGCAATCTGTTTGCGGAGTTTTCATACAGGAAATGATGATTATGTATTAGAGTTTTTTCTGCCTCCTGGGATTACTGAATTTCATGAACAGAAGACGCTGTTGGGATCCATATTGTCAGCAATGAAACAGCATTTTCAGAGTCTTAACATTGCTGCTGGTGTTGAACTCGAGGAAAATGGTTCAATTGAAATTATTGAAGCAACTGATGAAGGCATTCGTCTTAGAACCGAATCTATTCCTATTGCTCAATCTATTAAATCACCACCTAGACCCGATGCCTCACCAAATATGGAGGAGGAGGAGGAGGGAGTACCACAGGATCCATCAGAGGTTCATGGTGAAAATGTAGGCAGAAGTGTTGATCCGATGTCTACCTTAGGGAATAAAAACATAAAGAAACCCTCAGAGAGGAAACGTGGAAAAACTGAGAAATCAATTAGTCTTGAAGTTCTACAACGTTATTTTGCTGGGAGTCTTAAAGATGCTGCAAAGAGCCTTGGCGTTTGCCCTACTACAATGAAGCGTATCTGCAGGCAGCATGGGATATCTCGTTGGCCATCTCGAAAGATTAACAAGGTTAACCGTTCCCTATCTAAGCTCAAACGTGTTATTGAATCTGTCCAAGGCGCTGAAGGAGCGTTTGATTTGAATTCTCTTGGTAATAATCAACTTCCAATTGTTAGTTCATTTCCTGAGCCTTCTACTCCGAATAAGTCCAGCCAGCAATGCTCGTTAAGCAATAGGCCGTCAGAGCCTCAGATGAAAGAGAATGAATTTGATGCCTCTAAAGTATCAGAAACAAACATACAGATTGTAATGGAAAATCAATTGCTAGGAGGAAGGAAACATAGTCTTGAGAAAGAAGTGATTAATGGTAAAGGCGTGACTATTCAGGAAATTGGAAAGGACCGAAAAAGGAATAGAACTAGGAGTGGCTCGAGCGAAGATAGCACAAACCCTACTTCTCATGGTTCATGCCACGGTAGTCCCCCAATTGAAATTCCAACCATAAAAGATCCGTTCATTCCATCCAACAATGAACAACATGTTGTGTTGAGGGGATCGCCAGAGTCGGGAATGCAGCCGACTAATGCTTTAAACTCTCCAACTTCTCACCGTATGCCCGACAATGTGATAGCAGAACTTCAAGAGCCGTGTGGAGGATTGTTAATAGAGGACGCTGGAACTGAGGCCATTTTGGAGGATCTGGCTCCAGAAGCTTGTGGTAACAACTTTCCAGGTTCATCACATCTGGCTCCTCCTAAACAATGCATTGATATCATTAATAATTCAGTGACGCCTTTTGCAGCTAGAAAAGAAATGAAGACTGTAACTATTAAGGCAACATATAGAGAAGATATCATAAGGTTTAGGGTCTCTTTGAACTGTGGCATTGTGGAATTGAAAGAAGAAGTTTCCAAAAGATTGAAACTTGAGGTTGGCACATTTGATATCAAGTACATGGACGATGATAATGAATGGGTTTTGATAGCATGTGATGCAGACCTTCAGGAGTGCATGTATGTTTCAAGATCATCCGGAGGAAGCAACATAATCCGGGTTTTGGTGCATGACATAACATCAAATCTTGGAAGCTCCTGTGAGAGTTCAGGGGAGTGA
Protein sequence

>MsaT004999.1

MSEPEEENQDFPPKTKTSLEEHGCSMDFDLDLETPWPLDHMSFITNPMSPFLFSNISDQPSSPLWTFTDGEDGKLAASAFSDCHKIFSYDSNSITEKPVEKDDNKTLLPPLLPPIENLDGYCVIKEKMTQALRYFKEWTELNVLAQVWAPVRNGNRYVLTTSGQPFVLDPHSNGLNQYRTVSLMYMFSVDGENDGTLGLPGRVFQQKLPEWSPNVLYYSNKEYPRRDHAQHYNVRGTLALPVFEPSLQSCIGVIELIMTSLKINYAPEVEKICKALEAVNLRSSEFLDHPFTQICNEGRQNALSEILEILTVVCETHNLPLAQTWVPCRHRSVLAHGGGFKKSCSSFDGSCMGQVCMSTTEAAAYIVDAHLWGFREACVEHHLQHGQGVAGRAFLSQNMCFCTNITKFCKTDYPLVHYALMFGLTSCFAICLRSFHTGNDDYVLEFFLPPGITEFHEQKTLLGSILSAMKQHFQSLNIAAGVELEENGSIEIIEATDEGIRLRTESIPIAQSIKSPPRPDASPNMEEEEEGVPQDPSEVHGENVGRSVDPMSTLGNKNIKKPSERKRGKTEKSISLEVLQRYFAGSLKDAAKSLGVCPTTMKRICRQHGISRWPSRKINKVNRSLSKLKRVIESVQGAEGAFDLNSLGNNQLPIVSSFPEPSTPNKSSQQCSLSNRPSEPQMKENEFDASKVSETNIQIVMENQLLGGRKHSLEKEVINGKGVTIQEIGKDRKRNRTRSGSSEDSTNPTSHGSCHGSPPIEIPTIKDPFIPSNNEQHVVLRGSPESGMQPTNALNSPTSHRMPDNVIAELQEPCGGLLIEDAGTEAILEDLAPEACGNNFPGSSHLAPPKQCIDIINNSVTPFAARKEMKTVTIKATYREDIIRFRVSLNCGIVELKEEVSKRLKLEVGTFDIKYMDDDNEWVLIACDADLQECMYVSRSSGGSNIIRVLVHDITSNLGSSCESSGE*