AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005047

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005047 MtrunA17_Chr1g0203671 99.070 753 7 0 1 753 40 792 0.0 1544
MsaG005047 MtrunA17_Chr8g0350321 66.317 763 226 12 1 751 64 807 0.0 993
MsaG005047 MtrunA17_Chr7g0246631 65.706 729 221 9 30 751 69 775 0.0 975
MsaG005047 MtrunA17_Chr6g0472471 58.409 767 292 12 1 753 15 768 0.0 877
MsaG005047 MtrunA17_Chr2g0322731 53.678 734 301 10 21 750 67 765 0.0 739
MsaG005047 MtrunA17_Chr4g0024351 51.907 734 317 11 21 750 28 729 0.0 728
MsaG005047 MtrunA17_Chr6g0481041 46.133 724 349 15 47 750 21 723 0.0 628
MsaG005047 MtrunA17_Chr5g0410711 41.351 740 403 16 24 750 57 778 0.0 585
MsaG005047 MtrunA17_Chr2g0331581 41.954 696 350 11 60 750 143 789 0.0 563
MsaG005047 MtrunA17_Chr5g0394111 37.158 732 356 12 29 750 9 646 2.98e-155 466
MsaG005047 MtrunA17_Chr7g0224451 40.755 503 216 14 55 548 15 444 7.30e-114 352
MsaG005047 MtrunA17_Chr2g0292731 32.302 517 322 12 247 750 47 548 6.08e-87 285
MsaG005047 MtrunA17_Chr2g0292741 31.384 513 327 11 247 750 39 535 4.22e-82 271
MsaG005047 MtrunA17_Chr7g0224481 38.817 389 167 10 55 436 15 339 6.52e-78 254
MsaG005047 MtrunA17_Chr7g0224491 46.082 319 139 10 439 750 1 293 4.26e-75 245
MsaG005047 MtrunA17_Chr2g0292761 30.064 622 343 19 137 750 3 540 9.97e-71 241
MsaG005047 MtrunA17_Chr5g0419821 55.118 127 56 1 51 176 28 154 2.68e-42 151
MsaG005047 MtrunA17_Chr2g0319671 31.801 261 133 5 262 487 18 268 1.42e-35 137
MsaG005047 MtrunA17_Chr2g0319681 36.017 236 135 6 520 750 1 225 2.01e-32 126
MsaG005047 MtrunA17_Chr2g0319701 35.146 239 131 8 520 750 1 223 2.37e-30 120
MsaG005047 MtrunA17_Chr1g0205981 32.110 218 110 5 111 320 1 188 7.16e-24 100
MsaG005047 MtrunA17_Chr2g0319691 39.062 128 70 3 375 500 1 122 8.41e-23 95.5
MsaG005047 MtrunA17_Chr7g0224461 45.312 128 46 4 635 751 1 115 1.62e-21 90.5
MsaG005047 MtrunA17_Chr2g0304191 28.244 131 93 1 270 399 1 131 1.35e-15 74.3
MsaG005047 MtrunA17_Chr7g0265521 38.043 92 56 1 51 142 56 146 9.59e-12 65.5
MsaG005047 MtrunA17_Chr1g0179581 39.326 89 44 3 52 133 24 109 6.96e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005047 AT4G00730.1 62.973 740 240 9 12 751 94 799 0.0 914
MsaG005047 AT3G61150.1 60.898 757 234 13 23 750 80 803 0.0 899
MsaG005047 AT4G04890.2 53.117 738 318 12 15 750 24 735 0.0 769
MsaG005047 AT4G04890.1 53.117 738 318 12 15 750 24 735 0.0 769
MsaG005047 AT5G52170.3 51.643 761 278 13 1 752 1 680 0.0 748
MsaG005047 AT5G52170.1 51.643 761 278 13 1 752 1 680 0.0 748
MsaG005047 AT5G52170.2 51.305 766 278 14 1 752 1 685 0.0 742
MsaG005047 AT1G05230.8 51.565 735 304 12 20 750 30 716 0.0 740
MsaG005047 AT1G05230.4 51.565 735 304 12 20 750 30 716 0.0 740
MsaG005047 AT1G05230.9 51.565 735 304 12 20 750 30 716 0.0 740
MsaG005047 AT1G05230.10 51.565 735 304 12 20 750 30 716 0.0 740
MsaG005047 AT1G05230.7 51.565 735 304 12 20 750 30 716 0.0 740
MsaG005047 AT1G05230.11 51.565 735 304 12 20 750 30 716 0.0 740
MsaG005047 AT1G05230.2 51.565 735 304 12 20 750 30 716 0.0 740
MsaG005047 AT1G05230.6 51.565 735 304 12 20 750 30 716 0.0 740
MsaG005047 AT1G05230.5 51.565 735 304 12 20 750 30 716 0.0 740
MsaG005047 AT1G05230.1 51.565 735 304 12 20 750 30 716 0.0 740
MsaG005047 AT4G21750.2 50.921 760 323 12 15 750 24 757 0.0 735
MsaG005047 AT4G21750.1 50.921 760 323 12 15 750 24 757 0.0 735
MsaG005047 AT4G21750.3 50.921 760 323 12 15 750 24 757 0.0 735
MsaG005047 AT4G21750.4 50.921 760 323 12 15 750 24 757 0.0 735
MsaG005047 AT1G05230.3 51.429 735 303 13 20 750 30 714 0.0 733
MsaG005047 AT1G73360.1 46.273 711 339 14 55 750 31 713 0.0 607
MsaG005047 AT1G05230.12 50.325 616 265 11 15 626 25 603 0.0 605
MsaG005047 AT4G00730.2 60.649 493 167 7 12 504 94 559 0.0 589
MsaG005047 AT1G17920.2 44.587 702 335 14 59 750 24 681 0.0 586
MsaG005047 AT1G17920.1 44.587 702 335 14 59 750 24 681 0.0 586
MsaG005047 AT2G32370.1 40.028 727 377 12 31 747 40 717 0.0 545
MsaG005047 AT1G79840.3 39.860 715 358 15 48 752 34 686 1.57e-177 525
MsaG005047 AT1G79840.6 39.860 715 358 15 48 752 34 686 1.57e-177 525
MsaG005047 AT1G79840.1 39.860 715 358 15 48 752 92 744 6.26e-177 525
MsaG005047 AT1G79840.2 39.691 713 362 14 48 752 121 773 6.05e-176 524
MsaG005047 AT5G46880.3 39.730 740 391 18 40 750 93 806 3.69e-172 516
MsaG005047 AT5G46880.1 39.730 740 391 18 40 750 93 806 3.69e-172 516
MsaG005047 AT5G46880.2 39.730 740 391 18 40 750 93 806 3.69e-172 516
MsaG005047 AT4G25530.1 39.028 720 367 15 37 750 29 682 9.62e-165 492
MsaG005047 AT1G79840.4 40.000 585 285 13 48 622 92 620 3.40e-143 434
MsaG005047 AT3G03260.1 34.060 734 415 17 40 750 7 694 6.07e-137 421
MsaG005047 AT4G17710.1 34.907 699 368 16 56 750 88 703 1.16e-129 402
MsaG005047 AT5G17320.1 34.008 741 439 22 35 751 2 716 9.26e-123 384
MsaG005047 AT1G34650.1 32.642 723 440 16 46 750 12 705 6.65e-114 361
MsaG005047 AT3G03260.2 33.692 558 329 13 40 588 7 532 5.19e-102 324
MsaG005047 AT1G79840.5 38.013 463 223 11 48 501 92 499 7.63e-99 315
MsaG005047 AT5G07260.1 25.841 565 335 16 121 683 9 491 6.95e-49 181
MsaG005047 AT5G07260.2 26.018 565 329 16 121 683 9 486 9.98e-49 181
MsaG005047 AT1G30490.1 37.255 102 55 3 42 139 6 102 3.64e-11 67.4

Find 212 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCGTCCGTTGCTTATTTC+TGG 0.113009 1:-77078558 MsaT005047.1:CDS
CCGTTGCTTATTTCTGGTTT+TGG 0.171502 1:-77078552 MsaT005047.1:CDS
GGTGCTCTTCGAACAATATT+TGG 0.196521 1:+77080503 None:intergenic
AGATAGTCCTGTTTGGATTA+AGG 0.220607 1:-77080134 MsaT005047.1:CDS
GAAGACTATTCACCAGAATT+TGG 0.222863 1:+77077488 None:intergenic
TCATTTGGGTTCGACGATTC+TGG 0.227937 1:+77080902 None:intergenic
GTGCTTCATCTGCTAGAAAA+TGG 0.232062 1:-77078261 MsaT005047.1:CDS
TTTGATCTTGAAGGATTATC+TGG 0.248253 1:-77081207 MsaT005047.1:CDS
ATTTAATCTTCACCTTCATT+TGG 0.253274 1:+77080887 None:intergenic
CCACCACAGTTGTTACATAT+TGG 0.279446 1:+77080541 None:intergenic
ATGACCAACAACTTCTGTTC+TGG 0.284388 1:-77078289 MsaT005047.1:CDS
GATAGTCCTGTTTGGATTAA+GGG 0.285548 1:-77080133 MsaT005047.1:CDS
AAAATAAACAAGTCAAATTC+TGG 0.292831 1:-77080927 MsaT005047.1:CDS
TCCTGTTTGGATTAAGGGTT+TGG 0.303275 1:-77080128 MsaT005047.1:CDS
GGATTAGCCAATGATGATAT+TGG 0.305207 1:+77080403 None:intergenic
TTTAATCTTCACCTTCATTT+GGG 0.314861 1:+77080888 None:intergenic
CACAGTCGGATTCCAAATTC+TGG 0.317732 1:-77077500 MsaT005047.1:CDS
TATGTTGCACTCTTGCCTTC+AGG 0.326578 1:-77077622 MsaT005047.1:CDS
TCAAGCTCTTGAATTTGATT+AGG 0.330233 1:+77081120 None:intergenic
TTGGATTTGGCGCTCATAGA+TGG 0.334166 1:-77078533 MsaT005047.1:CDS
GCAACAAAGCCAGCAGGTTT+AGG 0.343857 1:+77080043 None:intergenic
CGGTTGATTTCTTCTTGTAT+TGG 0.345564 1:-77080067 MsaT005047.1:CDS
AACAGTCTTTGTCGTGAAAC+TGG 0.348067 1:+77078121 None:intergenic
CCAAGCCTTCACACTGCCTT+TGG 0.351820 1:+77078499 None:intergenic
GCTCTACCATCCTCAAATTC+TGG 0.354909 1:-77080376 MsaT005047.1:CDS
GTTTCCTTGCCCTTGACCTT+TGG 0.362517 1:+77078015 None:intergenic
ATGAATGGTTGTGCATTAGA+AGG 0.380812 1:+77079462 None:intergenic
TCTGGTCTGGAACTTGGTAT+TGG 0.381053 1:-77080358 MsaT005047.1:CDS
ACGATACCGGGAGGCTCGCC+AGG 0.388787 1:+77078172 None:intergenic
TAGTTCACCAATGGGATTGA+TGG 0.393674 1:-77080245 MsaT005047.1:CDS
TCTTAGCAAGAGACTTGGTT+TGG 0.395711 1:-77080950 MsaT005047.1:CDS
AATATTTGGCCAGGAATTGC+TGG 0.396823 1:+77080517 None:intergenic
AGCAGCATGCAGAAGGTGTA+TGG 0.397562 1:-77079521 MsaT005047.1:CDS
GGTATTGGAAGGAACGGTTT+TGG 0.397790 1:-77080343 MsaT005047.1:CDS
TTCAATGATTGCCCGTCAAC+TGG 0.404111 1:+77077673 None:intergenic
TGTTTGTTAAGGCACATATA+CGG 0.406272 1:+77080440 None:intergenic
GCCAAGGAACTTGTTTGTTA+AGG 0.406883 1:+77080429 None:intergenic
GCTGAAGCTACGAGAGAAAC+CGG 0.410030 1:-77080022 MsaT005047.1:CDS
AACTGACTCCATTGTGAGCT+TGG 0.411048 1:+77077459 None:intergenic
TGACCAACAACTTCTGTTCT+GGG 0.413733 1:-77078288 MsaT005047.1:CDS
AGATCTTGCATTGGCTGCTA+TGG 0.415072 1:-77080182 MsaT005047.1:CDS
TGTGGTGGACCAGCAATTCC+TGG 0.416505 1:-77080526 MsaT005047.1:CDS
CTCGGCAATCCTTTGCCAAT+GGG 0.418291 1:-77080304 MsaT005047.1:CDS
CTCAGACAGATAGTCCTGTT+TGG 0.418832 1:-77080141 MsaT005047.1:CDS
AAGAAATGGTGCTATACATT+TGG 0.420804 1:-77079695 MsaT005047.1:intron
TTTGTTGAATCAGACTCAAC+TGG 0.422317 1:-77080647 MsaT005047.1:intron
AGGATTCTCACTTGACGAAC+CGG 0.422609 1:+77079555 None:intergenic
TGTATTGGTCCTAAACCTGC+TGG 0.422932 1:-77080052 MsaT005047.1:CDS
CTTACCTTGGAGTAACCATT+TGG 0.423156 1:+77079396 None:intergenic
GTTGATGTTTCTGTTGAGAT+TGG 0.423253 1:-77079492 MsaT005047.1:CDS
AGCGATAACTTTGATCTTGA+AGG 0.423316 1:-77081216 MsaT005047.1:CDS
AGCCTTCACACTGCCTTTGG+AGG 0.424623 1:+77078502 None:intergenic
CATCACTCAGAGTCCCCATT+TGG 0.425038 1:+77078230 None:intergenic
TGCTTCATCTGCTAGAAAAT+GGG 0.434105 1:-77078260 MsaT005047.1:CDS
TCCTTCTGGCTGTATTGTGC+AGG 0.438832 1:-77079424 MsaT005047.1:CDS
TTAGCGCTGCAACATCTGTT+TGG 0.439034 1:-77078147 MsaT005047.1:CDS
TCAAATTCTGGTCTGGAACT+TGG 0.441450 1:-77080364 MsaT005047.1:CDS
AGCTTAGATACTGGTCTGCT+TGG 0.441475 1:-77081276 MsaT005047.1:CDS
ATGGAGGGGCAGAGTGAGAT+TGG 0.448707 1:-77081312 MsaT005047.1:CDS
AATTGAAAACGCCCGTCTAA+AGG 0.449088 1:-77080473 MsaT005047.1:CDS
AAGATCAAAGTTATCGCTTC+CGG 0.452078 1:+77081221 None:intergenic
AGCACTTGTGGAAACTTTGA+TGG 0.452399 1:-77079978 MsaT005047.1:CDS
ACCATCCTCAAATTCTGGTC+TGG 0.453392 1:-77080371 MsaT005047.1:CDS
GTTCAATTCGTCCTTTAGAC+GGG 0.453733 1:+77080462 None:intergenic
AAAATGGGACAGCCTCCAAA+TGG 0.457660 1:-77078245 MsaT005047.1:CDS
GGTGGTGCTGGCGATGGTGG+TGG 0.459096 1:-77077532 MsaT005047.1:CDS
ACATGCTTCTTCTACCAGCT+TGG 0.460496 1:+77078329 None:intergenic
TCCAAACCCTTAATCCAAAC+AGG 0.460668 1:+77080127 None:intergenic
CAAAGTTATCGCTTCCGGAC+CGG 0.465356 1:+77081226 None:intergenic
TGGCAAACCAATATCATCAT+TGG 0.472028 1:-77080410 MsaT005047.1:CDS
TGTTGATTCTGCAAGAAACT+TGG 0.473065 1:-77077729 MsaT005047.1:CDS
AATCAACAGTTTAGCACTTG+TGG 0.473233 1:-77079990 MsaT005047.1:CDS
CCTCGGCAATCCTTTGCCAA+TGG 0.475104 1:-77080305 MsaT005047.1:CDS
GGCTTTGCAATTGTTCCTGA+TGG 0.475420 1:-77077601 MsaT005047.1:CDS
TTGCCAATGGGACTTGATCT+CGG 0.479358 1:-77080292 MsaT005047.1:CDS
GCCTTAACAAACAAGTTCCT+TGG 0.480879 1:-77080430 MsaT005047.1:CDS
TGCAGCGCTAAGCACGATAC+CGG 0.481258 1:+77078159 None:intergenic
GCCTTCACACTGCCTTTGGA+GGG 0.482232 1:+77078503 None:intergenic
AAGTTCCAGACCAGAATTTG+AGG 0.483874 1:+77080366 None:intergenic
GGTGGTGGTTCAAGCTCCCT+CGG 0.484315 1:-77080322 MsaT005047.1:CDS
TCTGGCTACGGCATGTCAAA+TGG 0.484409 1:-77077568 MsaT005047.1:CDS
GATGACCCTGGCGAGCCTCC+CGG 0.485077 1:-77078178 MsaT005047.1:CDS
AGGAAAATGACAAGCTAAGA+GGG 0.487415 1:-77080597 MsaT005047.1:CDS
GTGGTGTATGCACCAGTTGA+CGG 0.489013 1:-77077685 MsaT005047.1:CDS
AAATGGGACAGCCTCCAAAT+GGG 0.490793 1:-77078244 MsaT005047.1:CDS
GGTTCAATTCGTCCTTTAGA+CGG 0.491156 1:+77080461 None:intergenic
AGGATTATCTGGTGATGATC+AGG 0.493656 1:-77081196 MsaT005047.1:CDS
AGGTCACTTGGATTGAGCAC+TGG 0.495140 1:-77078611 MsaT005047.1:CDS
GCTTAGATACTGGTCTGCTT+GGG 0.495575 1:-77081275 MsaT005047.1:CDS
AGCTGTAGAAGACTTCCTTC+TGG 0.496424 1:-77079438 MsaT005047.1:CDS
TTGCATTGTTGTAGGTCACT+TGG 0.496497 1:-77078623 MsaT005047.1:intron
GTTTGGATTAAGGGTTTGGA+TGG 0.496569 1:-77080124 MsaT005047.1:CDS
ACTGTTGATTATTACTATCC+CGG 0.497411 1:+77080003 None:intergenic
CCTGGTATTAGTTCACCAAT+GGG 0.499291 1:-77080253 MsaT005047.1:CDS
CCAAAGGCAGTGTGAAGGCT+TGG 0.499979 1:-77078499 MsaT005047.1:CDS
GGATTCTCACTTGACGAACC+GGG 0.501488 1:+77079556 None:intergenic
GATGAGTATGAAAGCCGGTC+CGG 0.502821 1:-77081240 MsaT005047.1:CDS
CCCATTGGTGAACTAATACC+AGG 0.505164 1:+77080253 None:intergenic
CACTCAGAGTCCCCATTTGG+AGG 0.506413 1:+77078233 None:intergenic
TGATGTTCAGCTTGAGAGAT+CGG 0.507347 1:-77080212 MsaT005047.1:CDS
ACTGGTGATAGCTTAGATAC+TGG 0.508547 1:-77081285 MsaT005047.1:CDS
ATATTGAATCAAGAAGAGTA+TGG 0.509859 1:-77080091 MsaT005047.1:CDS
TTCACACTGCCTTTGGAGGG+TGG 0.511667 1:+77078506 None:intergenic
CAGAGTGAGATTGGTCTCAC+TGG 0.512660 1:-77081303 MsaT005047.1:CDS
AGTCTTCTACAGCTTATGAA+TGG 0.512664 1:+77079447 None:intergenic
GGTAATGATCCCTCTGGCTA+CGG 0.514599 1:-77077580 MsaT005047.1:CDS
TTCGTCAAGTGAGAATCCTT+CGG 0.514988 1:-77079551 MsaT005047.1:CDS
TATGGATGAATTATTAAAGA+TGG 0.515193 1:-77080164 MsaT005047.1:CDS
TGAGAACATAATGCTTAGGC+AGG 0.515881 1:-77080617 MsaT005047.1:CDS
ATTGCTTTCACCACTAGTCC+CGG 0.516885 1:-77079574 MsaT005047.1:CDS
AGTTCACCAATGGGATTGAT+GGG 0.519574 1:-77080244 MsaT005047.1:CDS
TAGAAGAAGCATGTTGAAGC+TGG 0.521232 1:-77078320 MsaT005047.1:CDS
TCTTCGAACAATATTTGGCC+AGG 0.521570 1:+77080508 None:intergenic
GGAGATGGTCGCTCAATGCC+TGG 0.523620 1:-77080271 MsaT005047.1:CDS
GCCTGGTATTAGTTCACCAA+TGG 0.525635 1:-77080254 MsaT005047.1:CDS
GTCTAATGGTGGACCCATGC+AGG 0.526297 1:-77078053 MsaT005047.1:CDS
AAAGGTGGTGCTGGCGATGG+TGG 0.526742 1:-77077535 MsaT005047.1:CDS
GGTCACTTGGATTGAGCACT+GGG 0.528082 1:-77078610 MsaT005047.1:CDS
TGCAGCTTTGATCTTCTGAA+TGG 0.531714 1:+77077408 None:intergenic
GTTCACATTGCCAAAGGTCA+AGG 0.536772 1:-77078025 MsaT005047.1:CDS
GGATACATCTTGCTCGGTGG+TGG 0.536827 1:-77077704 MsaT005047.1:CDS
TTTGCAGCATTGAGCCAAGC+TGG 0.536871 1:-77078343 MsaT005047.1:intron
GGAATCGGTGGAACAAGAAA+TGG 0.540658 1:-77079709 MsaT005047.1:CDS
CAGGAAAATGACAAGCTAAG+AGG 0.541163 1:-77080598 MsaT005047.1:CDS
ATGAAAGGCTTAGAAGCGAG+TGG 0.543168 1:-77078084 MsaT005047.1:CDS
CCAGAGGGATCATTACCATC+AGG 0.544598 1:+77077586 None:intergenic
AACCCCAGAACAGAAGTTGT+TGG 0.545437 1:+77078285 None:intergenic
GAATCGTCGAACCCAAATGA+AGG 0.545567 1:-77080899 MsaT005047.1:intron
GGTGGAAGTTTGCTCACAGT+CGG 0.546541 1:-77077514 MsaT005047.1:CDS
TTCTGTAAGCAGCATGCAGA+AGG 0.546904 1:-77079528 MsaT005047.1:CDS
GGCTCTAATAGATCTTGCAT+TGG 0.550601 1:-77080191 MsaT005047.1:CDS
TTTGAGGATGGTAGAGCCAT+GGG 0.555597 1:+77080382 None:intergenic
GTGATGATGGTCAGCGCAAG+AGG 0.555699 1:-77081167 MsaT005047.1:CDS
ATGTGAACCATCTCCTGCAT+GGG 0.556273 1:+77078040 None:intergenic
ATCATCATTGGCTAATCCCA+TGG 0.556384 1:-77080398 MsaT005047.1:CDS
GATCAGGATGGTGGTGATGA+TGG 0.558173 1:-77081180 MsaT005047.1:CDS
TCAAATGGTACTTTACAGAA+AGG 0.561442 1:-77077553 MsaT005047.1:CDS
TGGTGGACCCATGCAGGAGA+TGG 0.561659 1:-77078047 MsaT005047.1:CDS
CCTGATGGTAATGATCCCTC+TGG 0.562249 1:-77077586 MsaT005047.1:CDS
TGGTGTATGCACCAGTTGAC+GGG 0.562286 1:-77077684 MsaT005047.1:CDS
ATGGGACTTGATCTCGGAGA+TGG 0.563438 1:-77080286 MsaT005047.1:CDS
GTCTGGAACTTGGTATTGGA+AGG 0.563807 1:-77080354 MsaT005047.1:CDS
GGCATGAGAACATAATGCTT+AGG 0.566863 1:-77080621 MsaT005047.1:CDS
CAGAAAGGTGGTGCTGGCGA+TGG 0.567714 1:-77077538 MsaT005047.1:CDS
GCTTCAGCAACAAAGCCAGC+AGG 0.568459 1:+77080037 None:intergenic
TCCATTGTGAGCTTGGCAGT+TGG 0.569368 1:+77077466 None:intergenic
TTGGATCTTAGCAAGAGACT+TGG 0.569590 1:-77080955 MsaT005047.1:CDS
ACCAGATAAAACATCAAGGT+TGG 0.570465 1:+77079732 None:intergenic
ATTTGAGGATGGTAGAGCCA+TGG 0.573157 1:+77080381 None:intergenic
CTACTTCCCATCAATCCCAT+TGG 0.573723 1:+77080238 None:intergenic
ACTTTACAGAAAGGTGGTGC+TGG 0.574608 1:-77077544 MsaT005047.1:CDS
ACCCTCCAAAGGCAGTGTGA+AGG 0.587970 1:-77078504 MsaT005047.1:CDS
TTGGATGGATACATCTTGCT+CGG 0.589147 1:-77077710 MsaT005047.1:CDS
CCAATATGTAACAACTGTGG+TGG 0.591280 1:-77080541 MsaT005047.1:CDS
GATTCTGCAAGAAACTTGGA+TGG 0.592935 1:-77077725 MsaT005047.1:CDS
GACCAACAACTTCTGTTCTG+GGG 0.596269 1:-77078287 MsaT005047.1:CDS
GCAGCATGCAGAAGGTGTAT+GGG 0.596526 1:-77079520 MsaT005047.1:CDS
GAACTTGGTATTGGAAGGAA+CGG 0.597234 1:-77080349 MsaT005047.1:CDS
GATGGATCGCCACCCTCCAA+AGG 0.597522 1:-77078515 MsaT005047.1:CDS
GGAACAATTGCAAAGCCTGA+AGG 0.597670 1:+77077607 None:intergenic
TCCAGACCAGAATTTGAGGA+TGG 0.597964 1:+77080370 None:intergenic
GCAGCGCTAAGCACGATACC+GGG 0.598314 1:+77078160 None:intergenic
AATGTGAACCATCTCCTGCA+TGG 0.599819 1:+77078039 None:intergenic
ACTTGACGAACCGGGACTAG+TGG 0.600610 1:+77079564 None:intergenic
AGATCAAGTCCCATTGGCAA+AGG 0.604435 1:+77080295 None:intergenic
TCTCCGAGATCAAGTCCCAT+TGG 0.604534 1:+77080289 None:intergenic
TAGCATGATGAAAGAAGCAA+TGG 0.604769 1:-77080569 MsaT005047.1:CDS
TGCAGAAGGTGTATGGGCCG+TGG 0.605614 1:-77079514 MsaT005047.1:CDS
GGTGTGATCATCGTTTGAGA+TGG 0.606434 1:+77078458 None:intergenic
TTGAATGTGGTGATGAGTGG+TGG 0.609782 1:-77077655 MsaT005047.1:CDS
ATGATGATATTGGTTTGCCA+AGG 0.610133 1:+77080413 None:intergenic
CATGCTGCTTACAGAACCGA+AGG 0.611995 1:+77079535 None:intergenic
CTGAAGCTACGAGAGAAACC+GGG 0.615105 1:-77080021 MsaT005047.1:CDS
ATCATCGTTTGAGATGGAAG+AGG 0.615317 1:+77078464 None:intergenic
TAAGCAACGGACGATAGAGT+TGG 0.616243 1:+77078565 None:intergenic
TGTGGAAACTTTGATGGATG+CGG 0.616357 1:-77079972 MsaT005047.1:intron
TCCAACTGCCAAGCTCACAA+TGG 0.619453 1:-77077467 MsaT005047.1:CDS
TGAATCAGACTCAACTGGAA+AGG 0.621264 1:-77080642 MsaT005047.1:intron
GAGAGGATGAGTATGAAAGC+CGG 0.622277 1:-77081245 MsaT005047.1:CDS
TTCACATTGCCAAAGGTCAA+GGG 0.624942 1:-77078024 MsaT005047.1:CDS
ATGACATGAGAGTTATGACT+AGG 0.627138 1:-77078210 MsaT005047.1:CDS
TATGCCAAATGGTTACTCCA+AGG 0.627451 1:-77079400 MsaT005047.1:intron
ATTTGACATGCCGTAGCCAG+AGG 0.627533 1:+77077570 None:intergenic
TTCCACCAGATAAAACATCA+AGG 0.627792 1:+77079728 None:intergenic
ATTGCCAAAGGTCAAGGGCA+AGG 0.631892 1:-77078019 MsaT005047.1:CDS
TGAATCAAGAAGAGTATGGA+CGG 0.632793 1:-77080087 MsaT005047.1:CDS
GCAATTTCAGATGCATACTG+AGG 0.637345 1:-77079601 MsaT005047.1:intron
TTTGATTAGGAGTGTGTCTG+TGG 0.637619 1:+77081133 None:intergenic
TTTGACATGCCGTAGCCAGA+GGG 0.639169 1:+77077571 None:intergenic
TGAAAGGCTTAGAAGCGAGT+GGG 0.641966 1:-77078083 MsaT005047.1:CDS
ATTGTGCAGGATATGCCAAA+TGG 0.645079 1:-77079411 MsaT005047.1:CDS
AATGGGACAGCCTCCAAATG+GGG 0.646605 1:-77078243 MsaT005047.1:CDS
AATGGTACTTTACAGAAAGG+TGG 0.653820 1:-77077550 MsaT005047.1:CDS
TTGAGATGGAAGAGGACATG+AGG 0.654253 1:+77078472 None:intergenic
GAGATGGTTCACATTGCCAA+AGG 0.654763 1:-77078031 MsaT005047.1:CDS
TCCTGCACAATACAGCCAGA+AGG 0.655286 1:+77079423 None:intergenic
CGATACCGGGAGGCTCGCCA+GGG 0.655976 1:+77078173 None:intergenic
CTCAACAGAAACATCAACCA+CGG 0.657444 1:+77079497 None:intergenic
TCATTGAATGTGGTGATGAG+TGG 0.663206 1:-77077658 MsaT005047.1:CDS
GCGCTAAGCACGATACCGGG+AGG 0.668252 1:+77078163 None:intergenic
CCAAAACCAGAAATAAGCAA+CGG 0.679772 1:+77078552 None:intergenic
GATGGATACATCTTGCTCGG+TGG 0.691053 1:-77077707 MsaT005047.1:CDS
CATTGGCAAAGGATTGCCGA+GGG 0.693173 1:+77080306 None:intergenic
TCTGCTTGGGAGAATGAGAG+AGG 0.693480 1:-77081262 MsaT005047.1:CDS
GGAAAATGACAAGCTAAGAG+GGG 0.705532 1:-77080596 MsaT005047.1:CDS
ATCTCAAACGATGATCACAC+CGG 0.708202 1:-77078456 MsaT005047.1:intron
TAAAACTTACATTAGCACGA+AGG 0.708878 1:+77077981 None:intergenic
TGACGGGCAATCATTGAATG+TGG 0.709763 1:-77077668 MsaT005047.1:CDS
CCATTGGCAAAGGATTGCCG+AGG 0.716261 1:+77080305 None:intergenic
TCTGGTGATGATCAGGATGG+TGG 0.726600 1:-77081189 MsaT005047.1:CDS
TTATCTGGTGATGATCAGGA+TGG 0.778846 1:-77081192 MsaT005047.1:CDS
AATCCAATATGTAACAACTG+TGG 0.783609 1:-77080544 MsaT005047.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 77077393 77081334 77077393 ID=MsaG005047
Chr1 mRNA 77077393 77081334 77077393 ID=MsaT005047.1;Parent=MsaG005047
Chr1 exon 77077393 77077776 77077393 ID=MsaT005047.1.exon8;Parent=MsaT005047.1
Chr1 CDS 77077393 77077776 77077393 ID=cds.MsaT005047.1;Parent=MsaT005047.1
Chr1 exon 77077991 77078358 77077991 ID=MsaT005047.1.exon7;Parent=MsaT005047.1
Chr1 CDS 77077991 77078358 77077991 ID=cds.MsaT005047.1;Parent=MsaT005047.1
Chr1 exon 77078457 77078631 77078457 ID=MsaT005047.1.exon6;Parent=MsaT005047.1
Chr1 CDS 77078457 77078631 77078457 ID=cds.MsaT005047.1;Parent=MsaT005047.1
Chr1 exon 77079401 77079613 77079401 ID=MsaT005047.1.exon5;Parent=MsaT005047.1
Chr1 CDS 77079401 77079613 77079401 ID=cds.MsaT005047.1;Parent=MsaT005047.1
Chr1 exon 77079696 77079797 77079696 ID=MsaT005047.1.exon4;Parent=MsaT005047.1
Chr1 CDS 77079696 77079797 77079696 ID=cds.MsaT005047.1;Parent=MsaT005047.1
Chr1 exon 77079973 77080656 77079973 ID=MsaT005047.1.exon3;Parent=MsaT005047.1
Chr1 CDS 77079973 77080656 77079973 ID=cds.MsaT005047.1;Parent=MsaT005047.1
Chr1 exon 77080900 77081017 77080900 ID=MsaT005047.1.exon2;Parent=MsaT005047.1
Chr1 CDS 77080900 77081017 77080900 ID=cds.MsaT005047.1;Parent=MsaT005047.1
Chr1 exon 77081117 77081334 77081117 ID=MsaT005047.1.exon1;Parent=MsaT005047.1
Chr1 CDS 77081117 77081334 77081117 ID=cds.MsaT005047.1;Parent=MsaT005047.1
Gene Sequence

>MsaT005047.1

ATGGAGGGGCAGAGTGAGATTGGTCTCACTGGTGATAGCTTAGATACTGGTCTGCTTGGGAGAATGAGAGAGGATGAGTATGAAAGCCGGTCCGGAAGCGATAACTTTGATCTTGAAGGATTATCTGGTGATGATCAGGATGGTGGTGATGATGGTCAGCGCAAGAGGAAGAAGAGATACCACAGACACACTCCTAATCAAATTCAAGAGCTTGAATCTTTCTTCAAGGAGTGTCCTCATCCTGATGAAAAACAAAGGTTGGATCTTAGCAAGAGACTTGGTTTGGAAAATAAACAAGTCAAATTCTGGTTCCAGAATCGTCGAACCCAAATGAAGACTCAACTGGAAAGGCATGAGAACATAATGCTTAGGCAGGAAAATGACAAGCTAAGAGGGGAGAATAGCATGATGAAAGAAGCAATGGTGAATCCAATATGTAACAACTGTGGTGGACCAGCAATTCCTGGCCAAATATTGTTCGAAGAGCACCAGATTAGAATTGAAAACGCCCGTCTAAAGGACGAATTGAACCGTATATGTGCCTTAACAAACAAGTTCCTTGGCAAACCAATATCATCATTGGCTAATCCCATGGCTCTACCATCCTCAAATTCTGGTCTGGAACTTGGTATTGGAAGGAACGGTTTTGGTGGTGGTTCAAGCTCCCTCGGCAATCCTTTGCCAATGGGACTTGATCTCGGAGATGGTCGCTCAATGCCTGGTATTAGTTCACCAATGGGATTGATGGGAAGTAGTAGTGATGTTCAGCTTGAGAGATCGGCTCTAATAGATCTTGCATTGGCTGCTATGGATGAATTATTAAAGATGGCTCAGACAGATAGTCCTGTTTGGATTAAGGGTTTGGATGGAGAGAGAGACATATTGAATCAAGAAGAGTATGGACGGTTGATTTCTTCTTGTATTGGTCCTAAACCTGCTGGCTTTGTTGCTGAAGCTACGAGAGAAACCGGGATAGTAATAATCAACAGTTTAGCACTTGTGGAAACTTTGATGGATGCGAATCGGTACGCAGATATGTTTCAGTCTATGATTGCTAGATCTGCCAACCTTGATGTTTTATCTGGTGGAATCGGTGGAACAAGAAATGGTGCTATACATTTGATGCATACTGAGGTTCAATTGCTTTCACCACTAGTCCCGGTTCGTCAAGTGAGAATCCTTCGGTTCTGTAAGCAGCATGCAGAAGGTGTATGGGCCGTGGTTGATGTTTCTGTTGAGATTGGTCATGATCCTTCTAATGCACAACCATTCATAAGCTGTAGAAGACTTCCTTCTGGCTGTATTGTGCAGGATATGCCAAATGGTTACTCCAAGGTCACTTGGATTGAGCACTGGGAGTATGATGAGAGTGTTGTTCACCAACTCTATCGTCCGTTGCTTATTTCTGGTTTTGGATTTGGCGCTCATAGATGGATCGCCACCCTCCAAAGGCAGTGTGAAGGCTTGGCTATCCTCATGTCCTCTTCCATCTCAAACGATGATCACACCGCATTGAGCCAAGCTGGTAGAAGAAGCATGTTGAAGCTGGCACAACGTATGACCAACAACTTCTGTTCTGGGGTTTGTGCTTCATCTGCTAGAAAATGGGACAGCCTCCAAATGGGGACTCTGAGTGATGACATGAGAGTTATGACTAGGAAAAATGTTGATGACCCTGGCGAGCCTCCCGGTATCGTGCTTAGCGCTGCAACATCTGTTTGGATGCCAGTTTCACGACAAAGACTGTTTGATTTTCTGCGTGATGAAAGGCTTAGAAGCGAGTGGGACATTTTGTCTAATGGTGGACCCATGCAGGAGATGGTTCACATTGCCAAAGGTCAAGGGCAAGGAAACTGCGTTTCTCTCCTTCGTGCTAATGCTGTTAATGCGAATGATAGCAGCATGTTGATTCTGCAAGAAACTTGGATGGATACATCTTGCTCGGTGGTGGTGTATGCACCAGTTGACGGGCAATCATTGAATGTGGTGATGAGTGGTGGAGATTCTGCATATGTTGCACTCTTGCCTTCAGGCTTTGCAATTGTTCCTGATGGTAATGATCCCTCTGGCTACGGCATGTCAAATGGTACTTTACAGAAAGGTGGTGCTGGCGATGGTGGTGGAAGTTTGCTCACAGTCGGATTCCAAATTCTGGTGAATAGTCTTCCAACTGCCAAGCTCACAATGGAGTCAGTTGATACTGTGAATAATCTCATCTCATGCACCATTCAGAAGATCAAAGCTGCACTTAGAGTTGCATAA
Protein sequence

>MsaT005047.1

MEGQSEIGLTGDSLDTGLLGRMREDEYESRSGSDNFDLEGLSGDDQDGGDDGQRKRKKRYHRHTPNQIQELESFFKECPHPDEKQRLDLSKRLGLENKQVKFWFQNRRTQMKTQLERHENIMLRQENDKLRGENSMMKEAMVNPICNNCGGPAIPGQILFEEHQIRIENARLKDELNRICALTNKFLGKPISSLANPMALPSSNSGLELGIGRNGFGGGSSSLGNPLPMGLDLGDGRSMPGISSPMGLMGSSSDVQLERSALIDLALAAMDELLKMAQTDSPVWIKGLDGERDILNQEEYGRLISSCIGPKPAGFVAEATRETGIVIINSLALVETLMDANRYADMFQSMIARSANLDVLSGGIGGTRNGAIHLMHTEVQLLSPLVPVRQVRILRFCKQHAEGVWAVVDVSVEIGHDPSNAQPFISCRRLPSGCIVQDMPNGYSKVTWIEHWEYDESVVHQLYRPLLISGFGFGAHRWIATLQRQCEGLAILMSSSISNDDHTALSQAGRRSMLKLAQRMTNNFCSGVCASSARKWDSLQMGTLSDDMRVMTRKNVDDPGEPPGIVLSAATSVWMPVSRQRLFDFLRDERLRSEWDILSNGGPMQEMVHIAKGQGQGNCVSLLRANAVNANDSSMLILQETWMDTSCSVVVYAPVDGQSLNVVMSGGDSAYVALLPSGFAIVPDGNDPSGYGMSNGTLQKGGAGDGGGSLLTVGFQILVNSLPTAKLTMESVDTVNNLISCTIQKIKAALRVA*