AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005050


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005050 MtrunA17_Chr1g0203721 84.459 296 36 3 225 518 596 883 6.93e-156 466
MsaG005050 MtrunA17_Chr1g0203721 90.741 216 20 0 1 216 1 216 4.99e-136 415
MsaG005050 MtrunA17_Chr6g0458391 34.899 298 151 10 65 334 227 509 9.03e-36 142
MsaG005050 MtrunA17_Chr6g0458391 53.175 126 56 2 65 190 93 215 1.42e-33 135
MsaG005050 MtrunA17_Chr5g0401121 50.526 95 46 1 69 162 62 156 3.59e-25 102
MsaG005050 MtrunA17_Chr3g0082121 53.684 95 43 1 69 162 49 143 2.41e-24 107
MsaG005050 MtrunA17_Chr4g0033611 35.669 157 89 5 27 180 47 194 9.96e-20 89.0
MsaG005050 MtrunA17_Chr2g0329421 38.776 98 59 1 65 162 29 125 2.94e-17 80.5
MsaG005050 MtrunA17_Chr7g0233911 31.250 144 85 2 65 194 36 179 6.62e-16 76.6
MsaG005050 MtrunA17_Chr7g0238821 37.037 108 65 3 69 175 3 108 1.24e-13 67.4
MsaG005050 MtrunA17_Chr7g0258861 31.206 141 93 3 65 204 12 149 2.09e-13 71.2
MsaG005050 MtrunA17_Chr3g0078041 29.677 155 94 2 66 205 53 207 7.91e-13 68.2
MsaG005050 MtrunA17_Chr2g0287061 31.618 136 72 2 65 192 185 307 1.01e-12 70.9
MsaG005050 MtrunA17_Chr2g0287061 32.877 146 63 4 65 209 31 142 1.52e-12 70.5
MsaG005050 MtrunA17_Chr3g0080351 36.036 111 58 1 66 163 79 189 1.33e-12 70.5
MsaG005050 MtrunA17_Chr2g0329361 36.111 108 61 2 65 167 63 167 1.35e-12 67.8
MsaG005050 MtrunA17_Chr5g0448761 37.736 106 62 2 66 167 63 168 3.21e-12 69.3
MsaG005050 MtrunA17_Chr2g0324011 33.333 141 89 4 65 204 12 148 7.20e-12 67.8
MsaG005050 MtrunA17_Chr2g0329031 40.000 95 54 2 69 163 20 111 1.46e-11 67.0
MsaG005050 MtrunA17_Chr2g0287651 34.314 102 63 3 69 167 81 181 5.14e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005050 AT3G07500.1 42.593 108 61 1 65 172 35 141 6.38e-19 85.9
MsaG005050 AT3G59470.3 40.816 98 57 1 65 162 72 168 6.99e-19 86.7
MsaG005050 AT3G59470.1 40.816 98 57 1 65 162 72 168 6.99e-19 86.7
MsaG005050 AT3G59470.2 40.816 98 57 1 65 162 84 180 8.73e-19 86.7
MsaG005050 AT4G38180.1 33.714 175 96 3 13 167 6 180 2.11e-16 82.8
MsaG005050 AT2G43280.1 30.345 145 86 3 66 195 25 169 3.90e-14 72.0
MsaG005050 AT4G12850.4 32.653 98 63 1 65 162 13 107 1.73e-13 68.2
MsaG005050 AT4G12850.2 32.653 98 63 1 65 162 13 107 1.86e-13 68.2
MsaG005050 AT5G18960.2 39.796 98 46 1 66 163 214 298 9.27e-13 71.2
MsaG005050 AT5G18960.2 35.354 99 51 1 65 163 45 130 1.21e-12 71.2
MsaG005050 AT5G18960.1 39.796 98 46 1 66 163 214 298 9.27e-13 71.2
MsaG005050 AT5G18960.1 35.354 99 51 1 65 163 45 130 1.21e-12 71.2
MsaG005050 AT3G06250.2 34.783 115 61 2 66 180 193 293 2.33e-12 70.1
MsaG005050 AT3G06250.2 35.354 99 51 1 65 163 30 115 3.22e-12 69.7
MsaG005050 AT3G06250.1 34.783 115 61 2 66 180 193 293 2.33e-12 70.1
MsaG005050 AT3G06250.1 35.354 99 51 1 65 163 30 115 3.22e-12 69.7
MsaG005050 AT3G06250.3 34.783 115 61 2 66 180 193 293 2.33e-12 70.1
MsaG005050 AT3G06250.3 35.354 99 51 1 65 163 30 115 3.22e-12 69.7
MsaG005050 AT2G32250.5 37.755 98 58 1 66 163 41 135 9.88e-12 68.2
MsaG005050 AT2G32250.2 37.755 98 58 1 66 163 41 135 1.04e-11 68.2
MsaG005050 AT2G32250.4 37.755 98 58 1 66 163 41 135 1.04e-11 68.2
MsaG005050 AT4G12850.1 32.653 98 63 1 65 162 13 107 1.09e-11 64.3
MsaG005050 AT2G32250.6 37.755 98 58 1 66 163 41 135 1.10e-11 68.2
MsaG005050 AT2G32250.1 37.755 98 58 1 66 163 41 135 1.10e-11 68.2
MsaG005050 AT2G32250.3 37.755 98 58 1 66 163 41 135 1.10e-11 68.2
MsaG005050 AT3G22170.2 28.415 183 95 4 2 163 16 183 1.23e-11 67.8
MsaG005050 AT3G22170.1 28.415 183 95 4 2 163 16 183 1.23e-11 67.8
MsaG005050 AT2G32250.8 37.895 95 56 1 69 163 3 94 2.71e-11 66.6
MsaG005050 AT2G43280.2 36.047 86 54 1 78 162 2 87 2.94e-11 62.8
MsaG005050 AT2G32250.7 37.895 95 56 1 69 163 3 94 3.04e-11 66.6

Find 110 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTCGGACTGCAACAATTT+TGG 0.148412 1:+77121859 None:intergenic
GGTTGACTCATATCATAATT+TGG 0.153971 1:-77121388 MsaT005050.1:CDS
AGGATTCTGAATGAGGATTT+TGG 0.176822 1:+77118952 None:intergenic
CAGCTTGCTACAACCATCTT+AGG 0.224643 1:+77120039 None:intergenic
CGGACTCATGGTATGTGTTT+TGG 0.233173 1:+77119135 None:intergenic
ACTTAAAGCTGAACTAGATT+TGG 0.264524 1:+77121362 None:intergenic
GATTATGTTGTGCTTCAAAA+TGG 0.267872 1:+77121490 None:intergenic
TTAGAAAAGATTGGATTAAT+AGG 0.287407 1:-77121683 MsaT005050.1:CDS
CTTACCTGCAAAGGGAGATT+TGG 0.310345 1:+77119613 None:intergenic
ACTTGGAACATCCAATAGTT+TGG 0.334138 1:-77121895 MsaT005050.1:CDS
CACATTCAAGTCCAAACTAT+TGG 0.341769 1:+77121884 None:intergenic
CATGCATTGAACCACTTCAT+TGG 0.347987 1:+77119362 None:intergenic
CCGAACGTTCTTCACGCTAA+TGG 0.356305 1:-77121843 MsaT005050.1:CDS
AACAACATGTTAGAATTGAT+TGG 0.361206 1:+77121453 None:intergenic
GTTGACTCATATCATAATTT+GGG 0.366463 1:-77121387 MsaT005050.1:CDS
TACATGGTATGGTGGTTTCT+AGG 0.366745 1:-77121653 MsaT005050.1:CDS
TCAACCAAATCTCCCTTTGC+AGG 0.367806 1:-77119617 MsaT005050.1:intron
CTTAAAGCTGAACTAGATTT+GGG 0.370982 1:+77121363 None:intergenic
GGCTTTGTCAACACAGTGTT+CGG 0.372860 1:-77121783 MsaT005050.1:CDS
GGTTCAATGTTAGAAAAGAT+TGG 0.384400 1:-77121692 MsaT005050.1:CDS
CCACAGACTTGTCAAGTTAT+CGG 0.386090 1:-77120001 MsaT005050.1:CDS
GGGATGTCTTCCAAAGCTAA+TGG 0.397500 1:-77120436 MsaT005050.1:CDS
TGAAATATTTCAAATGCTCT+TGG 0.400052 1:+77120372 None:intergenic
TTTGGTTGAGATGAAACATA+TGG 0.404147 1:+77119631 None:intergenic
AGAAGTTTGGCGGTTGGAAC+AGG 0.408816 1:+77119295 None:intergenic
TGTTGGTTAGAATAGAAGTT+TGG 0.411083 1:+77119282 None:intergenic
ACCACATTACCCATTAGCTT+TGG 0.425279 1:+77120426 None:intergenic
AACATTGAACCCCATCAATC+TGG 0.425557 1:+77121704 None:intergenic
ACAACATGTTAGAATTGATT+GGG 0.429453 1:+77121454 None:intergenic
AGTCAGAACTTCGGTATCTA+TGG 0.430265 1:+77118913 None:intergenic
TGGACATGCGACTGAGGATC+AGG 0.436653 1:-77119806 MsaT005050.1:CDS
GGAGACGAGAACTGGATGTT+TGG 0.438770 1:-77121559 MsaT005050.1:CDS
CATCGGAAGGAGACGAGAAC+TGG 0.445746 1:-77121567 MsaT005050.1:CDS
TAACAAGTATGCCAGATTGA+TGG 0.451658 1:-77121715 MsaT005050.1:CDS
AATGGCTCTCAAACTCAATC+TGG 0.454597 1:-77121825 MsaT005050.1:CDS
ATAATCAAACTGATTCAGAT+AGG 0.455395 1:-77119711 MsaT005050.1:CDS
AACAAGTATGCCAGATTGAT+GGG 0.458593 1:-77121714 MsaT005050.1:CDS
TGATGATCTAACACAAGATC+AGG 0.463119 1:+77119330 None:intergenic
TCCTCAGTCGCATGTCCATT+CGG 0.464228 1:+77119811 None:intergenic
GTCAACACAGTGTTCGGAAT+CGG 0.464277 1:-77121777 MsaT005050.1:CDS
GGATGTCTTCCAAAGCTAAT+GGG 0.465360 1:-77120435 MsaT005050.1:CDS
AGAAGCTAGTGATGAAATGA+AGG 0.466016 1:-77120457 MsaT005050.1:CDS
ATCTACATGAATACTATTTG+AGG 0.467176 1:+77119864 None:intergenic
TGTTGCATCTGATTATTGCT+TGG 0.479286 1:+77119110 None:intergenic
TGATAAAAGCTTACCTGCAA+AGG 0.481590 1:+77119604 None:intergenic
CAGACAACAGTATTTGAAGA+AGG 0.483624 1:+77119673 None:intergenic
AGCTTGCTACAACCATCTTA+GGG 0.484144 1:+77120040 None:intergenic
GAGGCAACTTGACGAAGTTA+TGG 0.484472 1:-77119938 MsaT005050.1:CDS
AATTCAAGATAACCCTAAGA+TGG 0.492105 1:-77120052 MsaT005050.1:CDS
AATTGACAGGACCGCTACAC+TGG 0.496426 1:-77119758 MsaT005050.1:CDS
AAGCAATAATCAGATGCAAC+AGG 0.498238 1:-77119108 MsaT005050.1:intron
ATGGTCACACAGCAAAATAC+AGG 0.502031 1:+77118932 None:intergenic
TCTGGTAATGCATCAGCAAA+TGG 0.509074 1:-77121807 MsaT005050.1:CDS
TAGGAGTAAGGTACATGGTA+TGG 0.509545 1:-77121664 MsaT005050.1:CDS
ACGATATCCCTCTCTGGAGC+AGG 0.509622 1:+77121623 None:intergenic
GATGGTGATCATAACAACTT+AGG 0.510043 1:-77121948 MsaT005050.1:CDS
GATAAAAGCTTACCTGCAAA+GGG 0.510969 1:+77119605 None:intergenic
CCATTAGCGTGAAGAACGTT+CGG 0.511332 1:+77121843 None:intergenic
ATTATGTTGTGCTTCAAAAT+GGG 0.511977 1:+77121491 None:intergenic
TGAGATGAAACATATGGAGA+TGG 0.517004 1:+77119637 None:intergenic
GAGATGAAACATATGGAGAT+GGG 0.517572 1:+77119638 None:intergenic
TAGAATAGAAGTTTGGCGGT+TGG 0.518493 1:+77119289 None:intergenic
AAAATACAGGATTCTGAATG+AGG 0.519697 1:+77118945 None:intergenic
ATCATTACTCGTCAACAAGA+TGG 0.520020 1:-77121528 MsaT005050.1:CDS
TTTCCTACGATATCCCTCTC+TGG 0.525239 1:+77121617 None:intergenic
TATTGCTTGGCACGGACTCA+TGG 0.526252 1:+77119123 None:intergenic
TGGTTAGAATAGAAGTTTGG+CGG 0.528921 1:+77119285 None:intergenic
AGATTGGATTAATAGGAGTA+AGG 0.541743 1:-77121676 MsaT005050.1:CDS
GATTATAATTCACCGTTGGT+TGG 0.543940 1:+77119728 None:intergenic
CAATAGTTTGGACTTGAATG+TGG 0.544732 1:-77121883 MsaT005050.1:CDS
TTGCCTTTCTCAACTATCGC+AGG 0.548639 1:-77119250 MsaT005050.1:intron
TTGTGTTGCTCAAGCTGTGG+AGG 0.559431 1:-77121409 MsaT005050.1:CDS
GTTTGATTATAATTCACCGT+TGG 0.560586 1:+77119724 None:intergenic
GTCAACCAGCACAAAAGAGA+TGG 0.564837 1:-77120318 MsaT005050.1:CDS
AAAGGCAAGGAGAACTCTGT+TGG 0.577643 1:+77119265 None:intergenic
CACAGTGTTCGGAATCGGTA+CGG 0.578865 1:-77121772 MsaT005050.1:CDS
TCCAAAGCTAATGGGTAATG+TGG 0.580465 1:-77120427 MsaT005050.1:CDS
AATCTTGATATTGAAAAGTG+AGG 0.582977 1:-77119902 MsaT005050.1:CDS
ACAGGACCGCTACACTGGAT+AGG 0.587540 1:-77119753 MsaT005050.1:CDS
AAATTTACCTGCTCCAGAGA+GGG 0.588352 1:-77121630 MsaT005050.1:CDS
AAGAGCTTCTGAATCTGTGG+AGG 0.588474 1:-77119977 MsaT005050.1:CDS
TACTGTGAAGAAACATCGGA+AGG 0.591189 1:-77121580 MsaT005050.1:CDS
CCATTCGGAAAGATCACCGC+AGG 0.593497 1:+77119826 None:intergenic
GAGTAAGCGAAGAAATTGAC+AGG 0.593964 1:-77119771 MsaT005050.1:CDS
ACACCTGCGATAGTTGAGAA+AGG 0.595685 1:+77119247 None:intergenic
GGCAAGAGCTTCTGAATCTG+TGG 0.597094 1:-77119980 MsaT005050.1:CDS
GAGTAAGGTACATGGTATGG+TGG 0.599166 1:-77121661 MsaT005050.1:CDS
CATCTGATTATTGCTTGGCA+CGG 0.603767 1:+77119115 None:intergenic
TCCGAATGGACATGCGACTG+AGG 0.607571 1:-77119812 MsaT005050.1:CDS
ACTGGATAGGTGCCAACCAA+CGG 0.611215 1:-77119740 MsaT005050.1:CDS
AGATGAAACATATGGAGATG+GGG 0.614637 1:+77119639 None:intergenic
TCAACAAGATGGAAAATACC+AGG 0.623065 1:-77121517 MsaT005050.1:CDS
CCTGCGGTGATCTTTCCGAA+TGG 0.627373 1:-77119826 MsaT005050.1:CDS
GTGCTTCAAAATGGGTAACC+TGG 0.628259 1:+77121499 None:intergenic
GATGAAACATATGGAGATGG+GGG 0.631905 1:+77119640 None:intergenic
TGCTTCAGAAAGTGAGCCTG+CGG 0.635679 1:-77119842 MsaT005050.1:CDS
CAACTTGACGAAGTTATGGA+AGG 0.645660 1:-77119934 MsaT005050.1:CDS
GAAGCTAGTGATGAAATGAA+GGG 0.646836 1:-77120456 MsaT005050.1:CDS
CCGATAACTTGACAAGTCTG+TGG 0.655057 1:+77120001 None:intergenic
ATTAATAGGAGTAAGGTACA+TGG 0.662022 1:-77121669 MsaT005050.1:CDS
TGCGATAGTTGAGAAAGGCA+AGG 0.663759 1:+77119252 None:intergenic
AGAGCTTCTGAATCTGTGGA+GGG 0.666121 1:-77119976 MsaT005050.1:CDS
GAAATTTACCTGCTCCAGAG+AGG 0.668278 1:-77121631 MsaT005050.1:CDS
GGTAATGCATCAGCAAATGG+AGG 0.677979 1:-77121804 MsaT005050.1:CDS
GTTATGCAGAGTCAGAACTT+CGG 0.680514 1:+77118904 None:intergenic
TTGGCACCTATCCAGTGTAG+CGG 0.682153 1:+77119747 None:intergenic
ACAAGTATGCCAGATTGATG+GGG 0.683870 1:-77121713 MsaT005050.1:CDS
AGGGTTATCAGTTTGCTGTG+AGG 0.684882 1:-77119957 MsaT005050.1:CDS
ATGAAACATATGGAGATGGG+GGG 0.693657 1:+77119641 None:intergenic
GCTCCAGAGAGGGATATCGT+AGG 0.703657 1:-77121620 MsaT005050.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 77118905 77121973 77118905 ID=MsaG005050
Chr1 mRNA 77118905 77121973 77118905 ID=MsaT005050.1;Parent=MsaG005050
Chr1 exon 77118905 77118991 77118905 ID=MsaT005050.1.exon6;Parent=MsaT005050.1
Chr1 CDS 77118905 77118991 77118905 ID=cds.MsaT005050.1;Parent=MsaT005050.1
Chr1 exon 77119109 77119168 77119109 ID=MsaT005050.1.exon5;Parent=MsaT005050.1
Chr1 CDS 77119109 77119168 77119109 ID=cds.MsaT005050.1;Parent=MsaT005050.1
Chr1 exon 77119251 77119403 77119251 ID=MsaT005050.1.exon4;Parent=MsaT005050.1
Chr1 CDS 77119251 77119403 77119251 ID=cds.MsaT005050.1;Parent=MsaT005050.1
Chr1 exon 77119618 77120092 77119618 ID=MsaT005050.1.exon3;Parent=MsaT005050.1
Chr1 CDS 77119618 77120092 77119618 ID=cds.MsaT005050.1;Parent=MsaT005050.1
Chr1 exon 77120306 77120477 77120306 ID=MsaT005050.1.exon2;Parent=MsaT005050.1
Chr1 CDS 77120306 77120477 77120306 ID=cds.MsaT005050.1;Parent=MsaT005050.1
Chr1 exon 77121364 77121973 77121364 ID=MsaT005050.1.exon1;Parent=MsaT005050.1
Chr1 CDS 77121364 77121973 77121364 ID=cds.MsaT005050.1;Parent=MsaT005050.1
Gene Sequence

>MsaT005050.1

ATGGATGGTGATCATAACAACTTAGGTAATGTGCATGTAGAAAATTTTGATGTTGGACTTGGAACATCCAATAGTTTGGACTTGAATGTGGACCAAAATTGTTGCAGTCCGAACGTTCTTCACGCTAATGGCTCTCAAACTCAATCTGGTAATGCATCAGCAAATGGAGGCTTTGTCAACACAGTGTTCGGAATCGGTACGGTATTTGAGTCTGATGAACATGCATATAGATTCTATAACAAGTATGCCAGATTGATGGGGTTCAATGTTAGAAAAGATTGGATTAATAGGAGTAAGGTACATGGTATGGTGGTTTCTAGGAAATTTACCTGCTCCAGAGAGGGATATCGTAGGAAAGACAAGAGAGATTTTACTGTGAAGAAACATCGGAAGGAGACGAGAACTGGATGTTTGGCACACATGATCATTACTCGTCAACAAGATGGAAAATACCAGGTTACCCATTTTGAAGCACAACATAATCATGATGATATAAACCCAATCAATTCTAACATGTTGTTACTAGAGTTGCAAAATGAATTTTGTGTTGCTCAAGCTGTGGAGGTTGACTCATATCATAATTTGGGACCCAAATCTAGTTCAGCTTTAAAAGCTAGTGATGAAATGAAGGGATGTCTTCCAAAGCTAATGGGTAATGTGGTTGTGTTGAAACATGCTAGTGTTGCTTATACTCCAAGAGCATTTGAAATATTTCAGCAACGATATGAGAACTCTTTGAATGTTATTGTCAACCAGCACAAAAGAGATGGATATTTGTTTGAGGAGATTAAGCAATTTAAAATTCAAGATAACCCTAAGATGGTTGTAGCAAGCTGTTACAAAGATTTATGCCACAGACTTGTCAAGTTATCGGCAAGAGCTTCTGAATCTGTGGAGGGTTATCAGTTTGCTGTGAGGCAACTTGACGAAGTTATGGAAGGAGTGCAGAAAATCTTGATATTGAAAAGTGAGGAAGCTCAAGTTATCACCTCAAATAGTATTCATGTAGATGCTTCAGAAAGTGAGCCTGCGGTGATCTTTCCGAATGGACATGCGACTGAGGATCAGGATGAAAGTAACAGAGTAAGCGAAGAAATTGACAGGACCGCTACACTGGATAGGTGCCAACCAACGGTGAATTATAATCAAACTGATTCAGATAGGATTTTAAATGTAGAACCTTCTTCAAATACTGTTGTCTGCATTTCATGCCCCCCATCTCCATATGTTTCATCTCAACCAAATCTCCCTTTGCAGGGCTTATTTGGTTTTGAAACCAATGAAGTGGTTCAATGCATGTATGAACAGCCTGATCTTGTGTTAGATCATCAATCTAATACTAACCTGTTCCAACCGCCAAACTTCTATTCTAACCAACAGAGTTCTCCTTGCCTTTCTCAACTATCGCAGGAGCCTATAATCCAAAACACATACCATGAGTCCGTGCCAAGCAATAATCAGATGCAACAGGGTATGGATCTTGATATCCAAAATCCTCATTCAGAATCCTGTATTTTGCTGTGTGACCATAGATACCGAAGTTCTGACTCTGCATAA

Protein sequence

>MsaT005050.1

MDGDHNNLGNVHVENFDVGLGTSNSLDLNVDQNCCSPNVLHANGSQTQSGNASANGGFVNTVFGIGTVFESDEHAYRFYNKYARLMGFNVRKDWINRSKVHGMVVSRKFTCSREGYRRKDKRDFTVKKHRKETRTGCLAHMIITRQQDGKYQVTHFEAQHNHDDINPINSNMLLLELQNEFCVAQAVEVDSYHNLGPKSSSALKASDEMKGCLPKLMGNVVVLKHASVAYTPRAFEIFQQRYENSLNVIVNQHKRDGYLFEEIKQFKIQDNPKMVVASCYKDLCHRLVKLSARASESVEGYQFAVRQLDEVMEGVQKILILKSEEAQVITSNSIHVDASESEPAVIFPNGHATEDQDESNRVSEEIDRTATLDRCQPTVNYNQTDSDRILNVEPSSNTVVCISCPPSPYVSSQPNLPLQGLFGFETNEVVQCMYEQPDLVLDHQSNTNLFQPPNFYSNQQSSPCLSQLSQEPIIQNTYHESVPSNNQMQQGMDLDIQNPHSESCILLCDHRYRSSDSA*