AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005097

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005097 MtrunA17_Chr1g0204361 95.210 167 8 0 1 167 1 167 6.56e-114 321
MsaG005097 MtrunA17_Chr6g0474681 39.474 152 89 2 17 167 4 153 1.52e-35 122
MsaG005097 MtrunA17_Chr1g0204351 41.060 151 83 2 17 167 73 217 9.85e-32 114
MsaG005097 MtrunA17_Chr6g0474671 37.748 151 84 3 19 167 71 213 1.69e-29 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005097 AT5G51780.2 35.802 162 93 3 13 167 19 176 1.59e-25 97.4
MsaG005097 AT5G51780.1 35.256 156 90 3 19 167 2 153 1.71e-23 91.3
MsaG005097 AT1G12540.1 38.125 160 86 7 18 167 74 230 4.95e-22 89.7
MsaG005097 AT5G51790.4 31.875 160 91 5 18 167 26 177 6.17e-22 87.8
MsaG005097 AT5G51790.1 31.875 160 91 5 18 167 26 177 7.31e-22 87.8
MsaG005097 AT4G25410.1 32.258 155 87 3 24 167 48 195 8.95e-22 88.2
MsaG005097 AT5G51790.2 31.875 160 91 5 18 167 91 242 2.10e-21 87.8
MsaG005097 AT5G51790.3 31.875 160 91 5 18 167 91 242 2.50e-21 87.8
MsaG005097 AT4G25400.1 32.215 149 94 3 19 167 25 166 8.90e-21 84.7
MsaG005097 AT4G25400.2 32.215 149 94 3 19 167 25 166 1.06e-20 84.7
MsaG005097 AT1G62975.2 34.503 171 94 4 15 167 70 240 1.22e-20 85.9
MsaG005097 AT1G62975.1 34.503 171 94 4 15 167 70 240 1.45e-20 85.9

Find 29 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACGTTTATCAGATTTAA+TGG 0.200332 1:-77656019 MsaT005097.1:CDS
ACACACTTCACTACACTTGT+AGG 0.335290 1:+77656105 None:intergenic
TGTGTTACCAACCAAGTAAA+TGG 0.368730 1:-77655961 MsaT005097.1:CDS
GTCAAGATTGATCTAATTCC+AGG 0.380052 1:-77656084 MsaT005097.1:CDS
GAGAGATAAGATCAAAGAAA+TGG 0.392291 1:-77656172 MsaT005097.1:CDS
AGTTCAAGTAGGGTTGAAAC+TGG 0.407760 1:-77656147 MsaT005097.1:CDS
AAGACCTTGATGAGCTCAAA+AGG 0.412689 1:+77656389 None:intergenic
GAAATATCTTGCCATTTACT+TGG 0.419250 1:+77655950 None:intergenic
TATCTTGCCATTTACTTGGT+TGG 0.419948 1:+77655954 None:intergenic
TTTATGCTGATTCAGATCTT+CGG 0.455944 1:+77656520 None:intergenic
AAGAAATGGTTAGTTCAAGT+AGG 0.512409 1:-77656158 MsaT005097.1:CDS
AGAAATGGTTAGTTCAAGTA+GGG 0.521680 1:-77656157 MsaT005097.1:CDS
ATAACAATTTCCACCCCACC+TGG 0.524411 1:+77656066 None:intergenic
GTGGAAATTGTTATATGTAG+TGG 0.547521 1:-77656057 MsaT005097.1:CDS
ATGGAAATAATACTTCAAGA+AGG 0.552508 1:-77656000 MsaT005097.1:CDS
TGATCTAATTCCAGGTGGGG+TGG 0.577334 1:-77656076 MsaT005097.1:CDS
AGACCTTGATGAGCTCAAAA+GGG 0.578199 1:+77656390 None:intergenic
GCATAAAATATTAACTACAT+CGG 0.611900 1:-77656504 MsaT005097.1:CDS
ATTTCATACTATTAAATCTG+AGG 0.612912 1:-77655932 MsaT005097.1:intron
GATTGATCTAATTCCAGGTG+GGG 0.621637 1:-77656079 MsaT005097.1:CDS
AGATTGATCTAATTCCAGGT+GGG 0.625836 1:-77656080 MsaT005097.1:CDS
GCTCAAAAGGGAGTGAAGAA+CGG 0.633531 1:+77656402 None:intergenic
GTAGGGAAGTTGAAAAGCAA+AGG 0.634358 1:-77656457 MsaT005097.1:CDS
AAAAGGGAGTGAAGAACGGA+GGG 0.640895 1:+77656406 None:intergenic
CGTTCAGTATCAGATCACAT+AGG 0.655166 1:-77656252 MsaT005097.1:CDS
AGTATCAGATCACATAGGTG+AGG 0.667408 1:-77656247 MsaT005097.1:CDS
CAAAAGGGAGTGAAGAACGG+AGG 0.674543 1:+77656405 None:intergenic
GGGAAGTTGAAAAGCAAAGG+AGG 0.678696 1:-77656454 MsaT005097.1:CDS
AAGATTGATCTAATTCCAGG+TGG 0.682027 1:-77656081 MsaT005097.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 77654876 77656546 77654876 ID=MsaG005097
Chr1 mRNA 77654876 77656546 77654876 ID=MsaT005097.1;Parent=MsaG005097
Chr1 exon 77654876 77654887 77654876 ID=MsaT005097.1.exon3;Parent=MsaT005097.1
Chr1 CDS 77654876 77654887 77654876 ID=cds.MsaT005097.1;Parent=MsaT005097.1
Chr1 exon 77655933 77656280 77655933 ID=MsaT005097.1.exon2;Parent=MsaT005097.1
Chr1 CDS 77655933 77656280 77655933 ID=cds.MsaT005097.1;Parent=MsaT005097.1
Chr1 exon 77656394 77656546 77656394 ID=MsaT005097.1.exon1;Parent=MsaT005097.1
Chr1 CDS 77656394 77656546 77656394 ID=cds.MsaT005097.1;Parent=MsaT005097.1
Gene Sequence

>MsaT005097.1

ATGGCCGAAGATCTGAATCAGCATAAAATATTAACTACATCGGACAATGATGATAAAAAGATGATTCGTAGGGAAGTTGAAAAGCAAAGGAGGATGCAAATGTCTAGTCTATGTTCATCCCTCCGTTCTTCACTCCCTTTTGAGCTCATCAAGGGTAAGCGTTCAGTATCAGATCACATAGGTGAGGCTGCAAATTATGTCCAAATTTTGAAGCAGAAAATTAATGAACTTGAAATAAAGAGAGATAAGATCAAAGAAATGGTTAGTTCAAGTAGGGTTGAAACTGGAAATGAATTATCAGCAGATCCTACAAGTGTAGTGAAGTGTGTCAAGATTGATCTAATTCCAGGTGGGGTGGAAATTGTTATATGTAGTGGTTTTGAGGATTGTAGTTCACGTTTATCAGATTTAATGGAAATAATACTTCAAGAAGGATGTGATGTTGTTCATTGTGTTACCAACCAAGTAAATGGCAAGATATTTCATACTATTAAATCTGAGGGAGGAGAATGA
Protein sequence

>MsaT005097.1

MAEDLNQHKILTTSDNDDKKMIRREVEKQRRMQMSSLCSSLRSSLPFELIKGKRSVSDHIGEAANYVQILKQKINELEIKRDKIKEMVSSSRVETGNELSADPTSVVKCVKIDLIPGGVEIVICSGFEDCSSRLSDLMEIILQEGCDVVHCVTNQVNGKIFHTIKSEGGE*