AlfalfaGEDB | MsaG005098

M. sativa ssp. caerulea / MsaG005098

KEGG: -
GO: GO:0000976 GO:0000977 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0090575 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005098	XP_003592388.2	94.643	168	9	0	1	168	77	244	2.32e-111	327

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005098	sp\|Q9FLI1\|BH036_ARATH	46.243	173	77	5	1	160	4	173	1.09e-36	127

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005098	tr\|G7ID49\|G7ID49_MEDTR	94.643	168	9	0	1	168	77	244	1.11e-111	327

TFs/TRs:

Gene ID	Type	Classification
MsaG005098	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG003265	MsaG005098	0.807265	9.341418e-50	1.493590e-47
MsaG005098	MsaG006346	0.838098	6.465526e-57	2.378500e-54
MsaG005098	MsaG007176	0.830268	5.810797e-55	1.694624e-52
MsaG005098	MsaG010958	0.803140	6.760248e-49	9.816656e-47
MsaG005098	MsaG010990	0.816071	1.158851e-51	2.301757e-49
MsaG005098	MsaG012185	0.819449	2.019009e-52	4.376487e-50
MsaG005098	MsaG015664	0.845686	6.533885e-59	3.062588e-56
MsaG005098	MsaG017881	0.812426	7.332811e-51	1.328970e-48
MsaG005098	MsaG025138	0.821896	5.568217e-53	1.287705e-50
MsaG005098	MsaG028082	0.816597	8.848102e-52	1.781209e-49
MsaG005098	MsaG028652	0.836695	1.472888e-56	5.191238e-54
MsaG005098	MsaG029094	0.836834	1.357768e-56	4.805640e-54
MsaG005098	MsaG029279	0.809534	3.081378e-50	5.203337e-48
MsaG005098	MsaG046305	0.809455	3.203825e-50	5.399837e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005098	MtrunA17_Chr1g0204351	94.643	168	9	0	1	168	77	244	2.14e-115	327
MsaG005098	MtrunA17_Chr6g0474671	54.762	168	72	2	1	166	73	238	2.34e-55	174
MsaG005098	MtrunA17_Chr6g0474681	39.884	173	94	3	1	167	8	176	5.31e-33	115
MsaG005098	MtrunA17_Chr1g0204361	36.527	167	86	3	1	154	21	180	8.01e-28	102

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005098	AT5G51780.1	46.243	173	77	5	1	160	4	173	1.11e-37	127
MsaG005098	AT5G51780.2	46.243	173	77	5	1	160	27	196	3.02e-37	127
MsaG005098	AT4G25400.1	44.785	163	83	3	1	160	27	185	9.67e-36	123
MsaG005098	AT4G25410.1	43.636	165	83	4	3	157	47	211	2.81e-34	120
MsaG005098	AT4G25400.2	46.897	145	70	3	1	142	27	167	4.21e-33	116
MsaG005098	AT5G51790.1	41.818	165	88	4	1	157	29	193	1.55e-30	110
MsaG005098	AT5G51790.3	41.818	165	88	4	1	157	94	258	6.72e-30	110
MsaG005098	AT5G51790.4	43.333	150	77	4	1	142	29	178	5.01e-29	105
MsaG005098	AT5G51790.2	43.333	150	77	4	1	142	94	243	2.98e-28	105
MsaG005098	AT1G62975.1	33.152	184	99	3	1	160	76	259	2.92e-23	92.8
MsaG005098	AT1G12540.1	35.429	175	98	5	3	163	79	252	5.12e-22	89.4
MsaG005098	AT1G62975.2	34.337	166	85	3	1	142	76	241	7.53e-21	86.3

Find 32 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCCCCACTTTCTAAATTTC+TGG	0.103688	1:+77660506	MsaT005098.1:CDS
GAAGAGGGAGAAGGGATCTA+AGG	0.225427	1:-77660162	None:intergenic
CTGGAACAACTGATGAAATA+TGG	0.304748	1:+77660525	MsaT005098.1:CDS
ATAAGAAGAAAATGGTTCAT+AGG	0.350975	1:+77660092	None:intergenic
ACTAAATGATGGATTCATAT+TGG	0.351963	1:-77660766	None:intergenic
AAATCACTTGAAGAAGAATA+TGG	0.357120	1:+77660332	MsaT005098.1:CDS
TACTAGTACTTGCTTTATAG+AGG	0.425326	1:+77660479	MsaT005098.1:CDS
TGGAAGAACTAAGTTACAAA+AGG	0.434554	1:+77660352	MsaT005098.1:CDS
ATACCTCACACTGCACACTA+TGG	0.439476	1:-77660596	None:intergenic
CCACAGTTCTATTATTCTTG+TGG	0.455790	1:-77660443	None:intergenic
AAGAGGGAGAAGGGATCTAA+GGG	0.467872	1:-77660161	None:intergenic
TAATCTGAACAACTAAATGA+TGG	0.468144	1:-77660777	None:intergenic
TAAGAAGAAAATGGTTCATA+GGG	0.475156	1:+77660093	None:intergenic
TAGATCTAACTGAGCTAAGA+AGG	0.478104	1:+77660736	MsaT005098.1:CDS
GTTCCAGAAATTTAGAAAGT+GGG	0.518377	1:-77660509	None:intergenic
TTTATCTGATCAAATGAATG+AGG	0.525114	1:+77660296	MsaT005098.1:CDS
TGTTCCAGAAATTTAGAAAG+TGG	0.525663	1:-77660510	None:intergenic
CACAAGAATAATAGAACTGT+GGG	0.526811	1:+77660444	MsaT005098.1:CDS
GGAAGAACTAAGTTACAAAA+GGG	0.531740	1:+77660353	MsaT005098.1:CDS
GAAGCAAAGGAGACAAGAAA+TGG	0.533586	1:+77660123	MsaT005098.1:CDS
TCTCCCTCTTCACTTCATCA+AGG	0.533778	1:+77660174	MsaT005098.1:CDS
TCACCTTGATGAAGTGAAGA+GGG	0.538188	1:-77660177	None:intergenic
GATGAAGTGAAGAGGGAGAA+GGG	0.546212	1:-77660170	None:intergenic
AGTACTTGCTTTATAGAGGA+AGG	0.558027	1:+77660483	MsaT005098.1:CDS
TTCCAGAAATTTAGAAAGTG+GGG	0.570258	1:-77660508	None:intergenic
ATAGGGAAATTGAGAAGCAA+AGG	0.575199	1:+77660110	MsaT005098.1:CDS
TGATGAAGTGAAGAGGGAGA+AGG	0.577558	1:-77660171	None:intergenic
CTCAGTTAGATCTACACTGT+CGG	0.580625	1:-77660727	None:intergenic
TTCACCTTGATGAAGTGAAG+AGG	0.603402	1:-77660178	None:intergenic
CCACAAGAATAATAGAACTG+TGG	0.617517	1:+77660443	MsaT005098.1:CDS
ACTCCATAGTGTGCAGTGTG+AGG	0.655288	1:+77660593	MsaT005098.1:CDS
TAGTGAAACTACAAGAAACA+TGG	0.661078	1:-77660419	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	77660103	77660800	77660103	ID=MsaG005098
Chr1	mRNA	77660103	77660800	77660103	ID=MsaT005098.1;Parent=MsaG005098
Chr1	exon	77660103	77660195	77660103	ID=MsaT005098.1.exon1;Parent=MsaT005098.1
Chr1	CDS	77660103	77660195	77660103	ID=cds.MsaT005098.1;Parent=MsaT005098.1
Chr1	exon	77660285	77660614	77660285	ID=MsaT005098.1.exon2;Parent=MsaT005098.1
Chr1	CDS	77660285	77660614	77660285	ID=cds.MsaT005098.1;Parent=MsaT005098.1
Chr1	exon	77660717	77660800	77660717	ID=MsaT005098.1.exon3;Parent=MsaT005098.1
Chr1	CDS	77660717	77660800	77660717	ID=cds.MsaT005098.1;Parent=MsaT005098.1

Gene Sequence

>MsaT005098.1

ATGGTTCATAGGGAAATTGAGAAGCAAAGGAGACAAGAAATGGCTACACTTCATGCTTCCCTTAGATCCCTTCTCCCTCTTCACTTCATCAAGGGAAAACGTTCTTTATCTGATCAAATGAATGAGGCAGTGAATTACATAAATCACTTGAAGAAGAATATGGAAGAACTAAGTTACAAAAGGGATGAACTGAAAAAACTCTCTAATCCTAGCCTTAAAAATAAAAGCCATGTTTCTTGTAGTTTCACTATCCACAAGAATAATAGAACTGTGGGAATTGAAATCAGTACTAGTACTTGCTTTATAGAGGAAGGTGCCCCACTTTCTAAATTTCTGGAACAACTGATGAAATATGGACTTGATGTTGTTAGCTGCTTTTCTATTCAAGTCAATGGAAGATTACTCCATAGTGTGCAGTGTGAGGTTATCGATTCCGACAGTGTAGATCTAACTGAGCTAAGAAGGAATTTTTCCAATATGAATCCATCATTTAGTTGTTCAGATTAA

Protein sequence

>MsaT005098.1

MVHREIEKQRRQEMATLHASLRSLLPLHFIKGKRSLSDQMNEAVNYINHLKKNMEELSYKRDELKKLSNPSLKNKSHVSCSFTIHKNNRTVGIEISTSTCFIEEGAPLSKFLEQLMKYGLDVVSCFSIQVNGRLLHSVQCEVIDSDSVDLTELRRNFSNMNPSFSCSD*