AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005110 MtrunA17_Chr1g0204501 95.722 187 8 0 18 204 12 198 2.79e-124 349
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005110 AT5G19910.1 71.014 207 43 4 1 204 1 193 1.39e-89 261
MsaG005110 AT5G19910.2 62.025 237 43 5 1 204 1 223 5.26e-83 246

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATCACTATTTACCTTAT+TGG 0.153158 1:-77834404 None:intergenic
GGATCCTTGTATATATTCTT+TGG 0.257913 1:-77833091 None:intergenic
GAACCTAGTGCTGCGCTTCC+TGG 0.281659 1:+77834910 MsaT005110.1:CDS
ACTTAAAGTATCTTCAATAT+TGG 0.293258 1:+77834039 MsaT005110.1:CDS
ATGATAAATTTAATATATTC+AGG 0.332776 1:-77834068 None:intergenic
GTAGTGCATGCACAGGTGCC+TGG 0.346902 1:-77834951 None:intergenic
AACCGTTGTCGCCCATCATC+TGG 0.352900 1:-77833112 None:intergenic
CTAGGTTCTGGAAGTGGTCT+TGG 0.382881 1:-77834895 None:intergenic
AATGGCACATCCTACCAATA+AGG 0.389767 1:+77834390 MsaT005110.1:CDS
GGAAGCGCAGCACTAGGTTC+TGG 0.393441 1:-77834907 None:intergenic
TCTACTTCTGGAAGAACTAT+AGG 0.406034 1:+77834848 MsaT005110.1:CDS
GAAATACTTCAGCTGATAGA+AGG 0.409099 1:+77835082 MsaT005110.1:CDS
TTTGAGGACGAAGCATTCAT+TGG 0.411048 1:+77834014 MsaT005110.1:CDS
ATGCTTCGTCCTCAAAATAT+CGG 0.443322 1:-77834007 None:intergenic
TAGTGCTGCGCTTCCTGGTC+CGG 0.453206 1:+77834915 MsaT005110.1:CDS
ATAGACAGCAGTTCTACTTC+TGG 0.455964 1:+77834836 MsaT005110.1:CDS
TCCGGCCTCGACTCAATCCC+AGG 0.469209 1:+77834933 MsaT005110.1:CDS
TCACTATTTACCTTATTGGT+AGG 0.479734 1:-77834400 None:intergenic
GATTGAGTCGAGGCCGGACC+AGG 0.483294 1:-77834928 None:intergenic
TGATAAATTTAATATATTCA+GGG 0.495109 1:-77834067 None:intergenic
GGTGATGGTGGGGTATCTCT+TGG 0.497160 1:-77831436 None:intergenic
GGAAGAACTATAGGAACAAT+CGG 0.500395 1:+77834857 MsaT005110.1:CDS
GCAACGCTTGTAGCAGGTAC+TGG 0.502051 1:-77834976 None:intergenic
GCCTGGGATTGAGTCGAGGC+CGG 0.504361 1:-77834934 None:intergenic
TACAATTATACTTACGGTGA+TGG 0.506256 1:-77831451 None:intergenic
TTGAGGACGAAGCATTCATT+GGG 0.512149 1:+77834015 MsaT005110.1:CDS
ATTATACTTACGGTGATGGT+GGG 0.517738 1:-77831447 None:intergenic
GAAGAAGCTCAAGAAAATAG+AGG 0.520705 1:-77834345 None:intergenic
TAGTGCATGCACAGGTGCCT+GGG 0.521440 1:-77834950 None:intergenic
AATTATACTTACGGTGATGG+TGG 0.525463 1:-77831448 None:intergenic
ATATACAAGGATCCAGATGA+TGG 0.525951 1:+77833100 MsaT005110.1:CDS
GGACCAGGAAGCGCAGCACT+AGG 0.549090 1:-77834913 None:intergenic
GTCACAGCAACGCTTGTAGC+AGG 0.549168 1:-77834982 None:intergenic
GCAGCACTAGGTTCTGGAAG+TGG 0.553833 1:-77834901 None:intergenic
ATACCGTATGGCATTGGAGA+AGG 0.568373 1:-77835024 None:intergenic
GAAAACCATACAGTGGATGT+AGG 0.571854 1:-77833176 None:intergenic
TGGCCTCTAAAACTGAAAGC+GGG 0.590594 1:+77831410 MsaT005110.1:CDS
ACCATACAGTGGATGTAGGT+AGG 0.594759 1:-77833172 None:intergenic
ATCCAGATGATGGGCGACAA+CGG 0.595215 1:+77833110 MsaT005110.1:CDS
GCTCCTTCTCCAATGCCATA+CGG 0.595355 1:+77835021 MsaT005110.1:CDS
TATACAAGGATCCAGATGAT+GGG 0.614053 1:+77833101 MsaT005110.1:CDS
AGGTGCCTGGGATTGAGTCG+AGG 0.623010 1:-77834938 None:intergenic
TTATACTTACGGTGATGGTG+GGG 0.625857 1:-77831446 None:intergenic
ATGGCCTCTAAAACTGAAAG+CGG 0.638044 1:+77831409 MsaT005110.1:CDS
TAGGCCAAAGAATATATACA+AGG 0.644719 1:+77833087 MsaT005110.1:intron
TCAAGAAAATAGAGGCAGTG+AGG 0.646000 1:-77834337 None:intergenic
GGATGTAGGTAGGATTAGCA+AGG 0.649374 1:-77833162 None:intergenic
ACTGGTGGTAGTGCATGCAC+AGG 0.653824 1:-77834958 None:intergenic
ACGCTTGTAGCAGGTACTGG+TGG 0.714222 1:-77834973 None:intergenic
TTTGCACTACCGGAGCCAGG+GGG 0.746793 1:-77835048 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 77831409 77835116 77831409 ID=MsaG005110
Chr1 mRNA 77831409 77835116 77831409 ID=MsaT005110.1;Parent=MsaG005110
Chr1 exon 77831409 77831458 77831409 ID=MsaT005110.1.exon1;Parent=MsaT005110.1
Chr1 CDS 77831409 77831458 77831409 ID=cds.MsaT005110.1;Parent=MsaT005110.1
Chr1 exon 77833090 77833187 77833090 ID=MsaT005110.1.exon2;Parent=MsaT005110.1
Chr1 CDS 77833090 77833187 77833090 ID=cds.MsaT005110.1;Parent=MsaT005110.1
Chr1 exon 77833994 77834090 77833994 ID=MsaT005110.1.exon3;Parent=MsaT005110.1
Chr1 CDS 77833994 77834090 77833994 ID=cds.MsaT005110.1;Parent=MsaT005110.1
Chr1 exon 77834333 77834411 77834333 ID=MsaT005110.1.exon4;Parent=MsaT005110.1
Chr1 CDS 77834333 77834411 77834333 ID=cds.MsaT005110.1;Parent=MsaT005110.1
Chr1 exon 77834826 77835116 77834826 ID=MsaT005110.1.exon5;Parent=MsaT005110.1
Chr1 CDS 77834826 77835116 77834826 ID=cds.MsaT005110.1;Parent=MsaT005110.1
Gene Sequence

>MsaT005110.1

ATGGCCTCTAAAACTGAAAGCGGGAGTCCAAGAGATACCCCACCATCACCGCCAAAGAATATATACAAGGATCCAGATGATGGGCGACAACGGTTTTTGCTGGAACTGGAATTTGTTCAATGCCTTGCTAATCCTACCTACATCCACTATTTGGCTCAGAACCGATATTTTGAGGACGAAGCATTCATTGGGTACTTAAAGTATCTTCAATATTGGCAACGCCCTGAATATATTAAATTTATCATGTATCCTCACTGCCTCTATTTTCTTGAGCTTCTTCAGAATGCAAATTTTCGCAATGCAATGGCACATCCTACCAATAAGGAATTGACACATAGACAGCAGTTCTACTTCTGGAAGAACTATAGGAACAATCGGCTAAAACACATTTTACCAAGACCACTTCCAGAACCTAGTGCTGCGCTTCCTGGTCCGGCCTCGACTCAATCCCAGGCACCTGTGCATGCACTACCACCAGTACCTGCTACAAGCGTTGCTGTGACAACATCGTCTAGTCAAGCTCCTTCTCCAATGCCATACGGTATACCCCCTGGCTCCGGTAGTGCAAAAAATGACATGAGAAATACTTCAGCTGATAGAAGGAAAAGAAAGTGA
Protein sequence

>MsaT005110.1

MASKTESGSPRDTPPSPPKNIYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLKYLQYWQRPEYIKFIMYPHCLYFLELLQNANFRNAMAHPTNKELTHRQQFYFWKNYRNNRLKHILPRPLPEPSAALPGPASTQSQAPVHALPPVPATSVAVTTSSSQAPSPMPYGIPPGSGSAKNDMRNTSADRRKRK*