AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005152


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005152 MtrunA17_Chr1g0204951 94.737 190 10 0 2 191 1 190 1.02e-129 362
MsaG005152 MtrunA17_Chr1g0204971 85.263 190 28 0 2 191 1 190 1.24e-119 336
MsaG005152 MtrunA17_Chr5g0394621 65.104 192 63 2 2 191 1 190 3.39e-87 254
MsaG005152 MtrunA17_Chr5g0394631 62.842 183 64 2 2 182 1 181 7.74e-76 233
MsaG005152 MtrunA17_Chr5g0394631 58.376 197 68 6 1 191 213 401 6.75e-70 217
MsaG005152 MtrunA17_Chr7g0273661 47.436 156 77 2 20 173 4 156 2.24e-44 144
MsaG005152 MtrunA17_Chr7g0223351 43.373 166 91 3 23 188 7 169 2.56e-39 132
MsaG005152 MtrunA17_Chr7g0223361 39.766 171 101 2 18 188 2 170 3.10e-38 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005152 AT2G32020.1 53.892 167 72 3 23 188 18 180 4.94e-59 183
MsaG005152 AT2G32030.1 48.404 188 91 4 2 188 3 185 9.14e-58 180
MsaG005152 AT3G22560.1 41.954 174 94 3 19 188 2 172 1.35e-38 130

Find 36 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTCTTTAGCATCTGATT+TGG 0.255230 1:+78285445 None:intergenic
CCGTCTTCTTTGTTTGTATA+AGG 0.260514 1:+78285308 None:intergenic
TGCTATGAGCTCCAAGTATT+GGG 0.261207 1:-78285150 MsaT005152.1:CDS
TTCAGATCTTGATGATGTTT+TGG 0.285412 1:-78285375 MsaT005152.1:CDS
GAGATTTGCTTGCTATGTTT+TGG 0.329625 1:+78285273 None:intergenic
TAATGGGTCGAAGAGATATT+TGG 0.336769 1:+78285402 None:intergenic
ATCAAGATCTGAAAGACTAA+TGG 0.348689 1:+78285385 None:intergenic
TTTAGCATCTGATTTGGAAT+AGG 0.367598 1:+78285451 None:intergenic
ATTACCTTTGCCCCAATACT+TGG 0.396577 1:+78285139 None:intergenic
TGCCTTCGCGATCGAGCAAT+CGG 0.411073 1:-78285233 MsaT005152.1:CDS
CAGAGGGTGTTGGAGAAGGC+TGG 0.411839 1:-78285017 MsaT005152.1:CDS
ACATAGCAAGCAAATCTCTA+TGG 0.412043 1:-78285268 MsaT005152.1:CDS
AGGAACAAATCTGCTGAACT+TGG 0.442081 1:-78285176 MsaT005152.1:CDS
ATGCTATGAGCTCCAAGTAT+TGG 0.448265 1:-78285151 MsaT005152.1:CDS
CTTGAGCTTCAAGCCGCTCC+AGG 0.458231 1:+78285063 None:intergenic
TCAAGATCTGAAAGACTAAT+GGG 0.460408 1:+78285386 None:intergenic
TTCTCAGAGGGTGTTGGAGA+AGG 0.470170 1:-78285021 MsaT005152.1:CDS
CAGAAAATATCTGTTCATCA+AGG 0.472630 1:-78284973 MsaT005152.1:CDS
GCTTCAAGCCGCTCCAGGTA+TGG 0.487071 1:+78285068 None:intergenic
AGGTTCCCAAGTGCAATACT+TGG 0.527166 1:+78285328 None:intergenic
AGCTCCAAGTATTGGGGCAA+AGG 0.537498 1:-78285143 MsaT005152.1:CDS
TAGAAAATGTGGCTTCTCAG+AGG 0.540854 1:-78285034 MsaT005152.1:CDS
ATTTAAAGAGTTTCCATACC+TGG 0.542410 1:-78285081 MsaT005152.1:CDS
GCCTTCGCGATCGAGCAATC+GGG 0.544699 1:-78285232 MsaT005152.1:CDS
TGTGGCTTCTCAGAGGGTGT+TGG 0.549890 1:-78285027 MsaT005152.1:CDS
AGAAAATATCTGTTCATCAA+GGG 0.554840 1:-78284972 MsaT005152.1:CDS
TCAAGTTGATGTAGAAAATG+TGG 0.572167 1:-78285045 MsaT005152.1:CDS
AAGAGTTTCCATACCTGGAG+CGG 0.585589 1:-78285076 MsaT005152.1:CDS
AAGTGGCCAAGTATTGCACT+TGG 0.601796 1:-78285334 MsaT005152.1:CDS
AGAAAATGTGGCTTCTCAGA+GGG 0.613906 1:-78285033 MsaT005152.1:CDS
CACTTGTTTGACAACAAGTG+TGG 0.622968 1:+78285115 None:intergenic
CCTTATACAAACAAAGAAGA+CGG 0.623002 1:-78285308 MsaT005152.1:CDS
CACACTTGTTGTCAAACAAG+TGG 0.638200 1:-78285114 MsaT005152.1:CDS
ACCCGATTGCTCGATCGCGA+AGG 0.639229 1:+78285231 None:intergenic
GCTATGAGCTCCAAGTATTG+GGG 0.656803 1:-78285149 MsaT005152.1:CDS
AGTGGCCAAGTATTGCACTT+GGG 0.670287 1:-78285333 MsaT005152.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 78284917 78285492 78284917 ID=MsaG005152
Chr1 mRNA 78284917 78285492 78284917 ID=MsaT005152.1;Parent=MsaG005152
Chr1 exon 78284917 78285492 78284917 ID=MsaT005152.1.exon1;Parent=MsaT005152.1
Chr1 CDS 78284917 78285492 78284917 ID=cds.MsaT005152.1;Parent=MsaT005152.1
Gene Sequence

>MsaT005152.1

ATGATGGAAAAAACTGCTACCTATTCCAAATCAGATGCTAAAGAAGAGTGTTCTGTAGTTGACTTGAGCCAAATATCTCTTCGACCCATTAGTCTTTCAGATCTTGATGATGTTTTGGTGTGGACAAGTGATGAAAAAGTGGCCAAGTATTGCACTTGGGAACCTTATACAAACAAAGAAGACGGCATTGACTTCATCCAAAACATAGCAAGCAAATCTCTATGGTTCAGAGCAATTTGCCTTCGCGATCGAGCAATCGGGTGTATCGATTTCTTTTTGTGTTCGGATAGATGCAGGAACAAATCTGCTGAACTTGGCTATGCTATGAGCTCCAAGTATTGGGGCAAAGGTAATGCCACACTTGTTGTCAAACAAGTGGTTGAAGCTGCATTTAAAGAGTTTCCATACCTGGAGCGGCTTGAAGCTCAAGTTGATGTAGAAAATGTGGCTTCTCAGAGGGTGTTGGAGAAGGCTGGTTTTCAAAGGGAGGGTGTTCTCAGAAAATATCTGTTCATCAAGGGAAAAAGTAGAGATATGGTTATGTTCAGTATTCTCTCAAGTGAAGTTCAAATTTGA

Protein sequence

>MsaT005152.1

MMEKTATYSKSDAKEECSVVDLSQISLRPISLSDLDDVLVWTSDEKVAKYCTWEPYTNKEDGIDFIQNIASKSLWFRAICLRDRAIGCIDFFLCSDRCRNKSAELGYAMSSKYWGKGNATLVVKQVVEAAFKEFPYLERLEAQVDVENVASQRVLEKAGFQREGVLRKYLFIKGKSRDMVMFSILSSEVQI*