AlfalfaGEDB | MsaG005153

M. sativa ssp. caerulea / MsaG005153

KEGG: -
GO: -
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005153	XP_013469781.1	96.757	185	6	0	1	185	1	185	6.72e-129	370

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005153	sp\|P05332\|YP20_BACLI	33.929	168	102	3	23	184	8	172	7.63e-24	95.5

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005153	tr\|A0A072VP71\|A0A072VP71_MEDTR	96.757	185	6	0	1	185	1	185	3.21e-129	370

TFs/TRs:

Gene ID	Type	Classification
MsaG005153	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000431	MsaG005153	0.806501	1.352301e-49	2.123716e-47
MsaG000519	MsaG005153	0.824453	1.418178e-53	3.514570e-51
MsaG001258	MsaG005153	0.801800	1.272757e-48	1.792379e-46
MsaG001727	MsaG005153	0.806818	1.160145e-49	1.835516e-47
MsaG004108	MsaG005153	0.804269	3.951792e-49	5.890561e-47
MsaG005153	MsaG005154	0.870682	2.472258e-66	2.948350e-63
MsaG005153	MsaG005435	0.801437	1.509596e-48	2.108436e-46
MsaG005153	MsaG005438	0.807498	8.340764e-50	1.341094e-47
MsaG005153	MsaG006601	0.804016	4.457158e-49	6.605131e-47
MsaG005153	MsaG006777	0.818055	4.170905e-52	8.717975e-50
MsaG005153	MsaG008017	0.803809	4.919244e-49	7.254720e-47
MsaG005153	MsaG008996	0.806905	1.112160e-49	1.763196e-47
MsaG005153	MsaG010525	0.814823	2.189564e-51	4.213776e-49
MsaG005153	MsaG011599	0.811424	1.209023e-50	2.137764e-48
MsaG005153	MsaG012422	0.820363	1.250686e-52	2.777352e-50
MsaG005153	MsaG014956	0.806166	1.589765e-49	2.476863e-47
MsaG005153	MsaG014926	0.819950	1.554036e-52	3.413361e-50
MsaG005153	MsaG014927	0.825510	8.005818e-54	2.042357e-51
MsaG005153	MsaG015269	0.814621	2.426019e-51	4.644923e-49
MsaG005153	MsaG016348	0.804289	3.913425e-49	5.836191e-47
MsaG005153	MsaG016724	0.802172	1.068583e-48	1.517579e-46
MsaG005153	MsaG019731	0.805167	2.571455e-49	3.913726e-47
MsaG005153	MsaG020314	0.809046	3.916683e-50	6.536424e-48
MsaG005153	MsaG020580	0.802036	1.139028e-48	1.612622e-46
MsaG005153	MsaG020846	0.811062	1.447333e-50	2.536578e-48
MsaG005153	MsaG021575	0.808191	5.949564e-50	9.726825e-48
MsaG005153	MsaG023884	0.819635	1.831993e-52	3.990270e-50
MsaG005153	MsaG029340	0.808097	6.230575e-50	1.016316e-47
MsaG005153	MsaG030010	0.807322	9.087633e-50	1.455014e-47
MsaG005153	MsaG030471	0.817088	6.871949e-52	1.401085e-49
MsaG005153	MsaG031436	0.812656	6.533111e-51	1.190812e-48
MsaG005153	MsaG033371	0.826846	3.863893e-54	1.023034e-51
MsaG005153	MsaG035057	0.808151	6.068963e-50	9.912274e-48
MsaG005153	MsaG038269	0.804705	3.208016e-49	4.830300e-47
MsaG005153	MsaG040980	0.804729	3.171995e-49	4.778709e-47
MsaG005153	MsaG041039	0.823999	1.811148e-53	4.433290e-51
MsaG005153	MsaG041256	0.815735	1.376042e-51	2.709932e-49
MsaG005153	MsaG043980	0.804239	4.008027e-49	5.970271e-47
MsaG005153	MsaG044354	0.820478	1.177565e-52	2.622893e-50
MsaG005153	MsaG044377	0.808416	5.332610e-50	8.765113e-48
MsaG005153	MsaG044606	0.815588	1.482581e-51	2.908990e-49
MsaG005153	MsaG045021	0.808210	5.897112e-50	9.645234e-48
MsaG005153	MsaG045125	0.813840	3.601936e-51	6.762951e-49
MsaG005153	MsaG045522	0.829091	1.120021e-54	3.158169e-52
MsaG005153	MsaG046311	0.835631	2.735844e-56	9.338840e-54
MsaG005153	MsaG046384	0.808557	4.977226e-50	8.208993e-48
MsaG005153	MsaG046588	0.807932	6.753531e-50	1.097271e-47
MsaG005153	MsaG012695	0.800020	2.928040e-48	3.960826e-46
MsaG005153	MsaG020796	0.801669	1.354168e-48	1.901288e-46
MsaG005153	MsaG019920	0.802902	7.567800e-49	1.092900e-46
MsaG005153	MsaG032474	0.809367	3.345237e-50	5.625883e-48
MsaG005153	MsaG037384	0.804478	3.575530e-49	5.355445e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005153	MtrunA17_Chr1g0204971	96.757	185	6	0	1	185	1	185	6.18e-133	370
MsaG005153	MtrunA17_Chr1g0204951	84.865	185	28	0	1	185	1	185	3.60e-114	322
MsaG005153	MtrunA17_Chr5g0394621	62.903	186	65	2	1	184	1	184	8.91e-81	238
MsaG005153	MtrunA17_Chr5g0394631	60.109	183	69	2	1	181	1	181	3.44e-72	223
MsaG005153	MtrunA17_Chr5g0394631	54.124	194	81	5	1	188	208	399	6.38e-62	197
MsaG005153	MtrunA17_Chr7g0273661	48.718	156	75	2	19	172	4	156	2.83e-44	144
MsaG005153	MtrunA17_Chr7g0223361	39.881	168	99	2	17	184	2	167	1.83e-37	127
MsaG005153	MtrunA17_Chr7g0223351	41.212	165	94	3	22	186	7	168	2.16e-36	124

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005153	AT2G32020.1	52.727	165	73	3	22	185	18	178	1.30e-54	172
MsaG005153	AT2G32030.1	46.196	184	94	3	3	185	4	183	2.20e-54	171
MsaG005153	AT3G22560.1	41.420	169	92	2	18	182	2	167	4.67e-36	124

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGAAAATATCTGTTCATTA+AGG	0.174922	1:-78286823	MsaT005153.1:CDS
TGTGGTTGAAGCTGCATTTA+AGG	0.195666	1:-78286946	MsaT005153.1:CDS
TTCTTTGAGCTCCATGTATT+GGG	0.213171	1:-78287000	MsaT005153.1:CDS
CTCTTCTTTAGCATCTGATT+TGG	0.248198	1:+78287295	None:intergenic
ATTCTTTGAGCTCCATGTAT+TGG	0.293943	1:-78287001	MsaT005153.1:CDS
GCCTCAAGCCTCTCCAAGTA+TGG	0.329009	1:+78286918	None:intergenic
GAGATTTGCTTGCTATGTTT+TGG	0.329625	1:+78287123	None:intergenic
CCTTATACAAACAAAGAAAA+AGG	0.343403	1:-78287158	MsaT005153.1:CDS
TGCCTTCGCGATAGAGCAAT+TGG	0.346097	1:-78287083	MsaT005153.1:CDS
GGGCAAAGGGATTGCCACTA+TGG	0.352044	1:-78286979	MsaT005153.1:CDS
TCAAGATCAGAAAGACTAAT+TGG	0.352059	1:+78287236	None:intergenic
TTTAGCATCTGATTTGGAAT+AGG	0.367598	1:+78287301	None:intergenic
AATTGGGTGTACCGAGTTCC+AGG	0.375481	1:-78287066	MsaT005153.1:CDS
AGAAAATATCTGTTCATTAA+GGG	0.384110	1:-78286822	MsaT005153.1:CDS
ATTTAAGGAGTTTCCATACT+TGG	0.408342	1:-78286931	MsaT005153.1:CDS
ACATAGCAAGCAAATCTCTA+TGG	0.412043	1:-78287118	MsaT005153.1:CDS
CAAAGGGTGTTGGAGAAGGC+TGG	0.437360	1:-78286867	MsaT005153.1:CDS
TACCGAGTTCCAGGTGTGTT+CGG	0.452367	1:-78287057	MsaT005153.1:CDS
TAATTGGTCGAAGAGAGATT+TGG	0.457252	1:+78287252	None:intergenic
CGGGACAAATCTGCTCAACT+TGG	0.459831	1:-78287026	MsaT005153.1:CDS
GGCATCTATCCGAACACACC+TGG	0.486937	1:+78287048	None:intergenic
AGCTCCATGTATTGGGGCAA+AGG	0.494926	1:-78286993	MsaT005153.1:CDS
AATCCCTTTGCCCCAATACA+TGG	0.496567	1:+78286989	None:intergenic
AGGTGTGTTCGGATAGATGC+CGG	0.506000	1:-78287046	MsaT005153.1:CDS
TTCTCAAAGGGTGTTGGAGA+AGG	0.511828	1:-78286871	MsaT005153.1:CDS
GCCTTCGCGATAGAGCAATT+GGG	0.515394	1:-78287082	MsaT005153.1:CDS
GCTCCATGTATTGGGGCAAA+GGG	0.517436	1:-78286992	MsaT005153.1:CDS
ATCTTGATGATGTTATGTTG+TGG	0.524636	1:-78287220	MsaT005153.1:CDS
AGGTTCCCAAGTGCAATACT+TGG	0.527166	1:+78287178	None:intergenic
CAAGTTGAGCAGATTTGTCC+CGG	0.527345	1:+78287027	None:intergenic
GAAAATATCTGTTCATTAAG+GGG	0.536787	1:-78286821	MsaT005153.1:CDS
TGTGGCTTCTCAAAGGGTGT+TGG	0.546349	1:-78286877	MsaT005153.1:CDS
TCCATACTTGGAGAGGCTTG+AGG	0.557426	1:-78286919	MsaT005153.1:CDS
CACATGTTTCACAACCATAG+TGG	0.561077	1:+78286965	None:intergenic
GCTTGAGGCTCGAGTTGATG+TGG	0.569120	1:-78286904	MsaT005153.1:CDS
TGGAAAATGTGGCTTCTCAA+AGG	0.572207	1:-78286884	MsaT005153.1:CDS
AGGAGTTTCCATACTTGGAG+AGG	0.574247	1:-78286926	MsaT005153.1:CDS
ATCCGAACACACCTGGAACT+CGG	0.577234	1:+78287055	None:intergenic
TCTTTGAGCTCCATGTATTG+GGG	0.587210	1:-78286999	MsaT005153.1:CDS
GGAAAATGTGGCTTCTCAAA+GGG	0.588500	1:-78286883	MsaT005153.1:CDS
AAGTGGCCAAGTATTGCACT+TGG	0.595225	1:-78287184	MsaT005153.1:CDS
GGTGTGTTCGGATAGATGCC+GGG	0.599178	1:-78287045	MsaT005153.1:CDS
GTGGACTAGTGATGCAAAAG+TGG	0.603948	1:-78287201	MsaT005153.1:CDS
ACCCAATTGCTCTATCGCGA+AGG	0.616201	1:+78287081	None:intergenic
TCGAGTTGATGTGGAAAATG+TGG	0.669414	1:-78286895	MsaT005153.1:CDS
AGTGGCCAAGTATTGCACTT+GGG	0.670287	1:-78287183	MsaT005153.1:CDS
CACTATGGTTGTGAAACATG+TGG	0.680456	1:-78286964	MsaT005153.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	78286773	78287339	78286773	ID=MsaG005153
Chr1	mRNA	78286773	78287339	78286773	ID=MsaT005153.1;Parent=MsaG005153
Chr1	exon	78286773	78287339	78286773	ID=MsaT005153.1.exon1;Parent=MsaT005153.1
Chr1	CDS	78286773	78287339	78286773	ID=cds.MsaT005153.1;Parent=MsaT005153.1

Gene Sequence

>MsaT005153.1

ATGGAAGAAACTGCTACCTATTCCAAATCAGATGCTAAAGAAGAGTATTCTGTTGTTGACTTGAGCCAAATCTCTCTTCGACCAATTAGTCTTTCTGATCTTGATGATGTTATGTTGTGGACTAGTGATGCAAAAGTGGCCAAGTATTGCACTTGGGAACCTTATACAAACAAAGAAAAAGGCATTGACTTCATCCAAAACATAGCAAGCAAATCTCTATGGTTCAGAGCAATTTGCCTTCGCGATAGAGCAATTGGGTGTACCGAGTTCCAGGTGTGTTCGGATAGATGCCGGGACAAATCTGCTCAACTTGGCTATTCTTTGAGCTCCATGTATTGGGGCAAAGGGATTGCCACTATGGTTGTGAAACATGTGGTTGAAGCTGCATTTAAGGAGTTTCCATACTTGGAGAGGCTTGAGGCTCGAGTTGATGTGGAAAATGTGGCTTCTCAAAGGGTGTTGGAGAAGGCTGGTTTTGAAAGGGAAGGTGTTCTCAGAAAATATCTGTTCATTAAGGGGAAAAGTATAGATATTGTTATGTTCAGTATTCTCTCAGTGAAGTTATAA

Protein sequence

>MsaT005153.1

MEETATYSKSDAKEEYSVVDLSQISLRPISLSDLDDVMLWTSDAKVAKYCTWEPYTNKEKGIDFIQNIASKSLWFRAICLRDRAIGCTEFQVCSDRCRDKSAQLGYSLSSMYWGKGIATMVVKHVVEAAFKEFPYLERLEARVDVENVASQRVLEKAGFEREGVLRKYLFIKGKSIDIVMFSILSVKL*