Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG005244 | XP_003592443.1 | 60.294 | 68 | 27 | 0 | 31 | 98 | 1 | 68 | 2.34e-18 | 86.7 |
MsaG005244 | XP_003592443.1 | 67.308 | 104 | 20 | 1 | 32 | 121 | 73 | 176 | 1.50e-36 | 132 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG005244 | sp|O48686|SNL3_ARATH | 43.820 | 89 | 49 | 1 | 27 | 114 | 12 | 100 | 3.35e-16 | 76.6 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG005244 | tr|G7IE24|G7IE24_MEDTR | 60.294 | 68 | 27 | 0 | 31 | 98 | 1 | 68 | 1.12e-18 | 86.7 |
MsaG005244 | tr|G7IE24|G7IE24_MEDTR | 67.308 | 104 | 20 | 1 | 32 | 121 | 73 | 176 | 7.14e-37 | 132 |
Gene ID | Type | Classification |
---|---|---|
MsaG005244 | TR | Others |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000352 | MsaG005244 | 0.814974 | 2.026986e-51 | 3.915854e-49 |
MsaG000760 | MsaG005244 | 0.807748 | 7.386407e-50 | 1.194765e-47 |
MsaG002021 | MsaG005244 | 0.802643 | 8.553351e-49 | 1.227943e-46 |
MsaG003423 | MsaG005244 | 0.821470 | 6.976030e-53 | 1.595111e-50 |
MsaG003529 | MsaG005244 | 0.807048 | 1.037965e-49 | 1.651117e-47 |
MsaG003964 | MsaG005244 | 0.820944 | 9.212222e-53 | 2.077389e-50 |
MsaG005244 | MsaG005957 | 0.839987 | 2.106343e-57 | 8.219331e-55 |
MsaG005244 | MsaG007526 | 0.813247 | 4.857335e-51 | 8.985072e-49 |
MsaG005244 | MsaG007652 | 0.806925 | 1.101548e-49 | 1.747180e-47 |
MsaG005244 | MsaG007767 | 0.824036 | 1.775530e-53 | 4.350480e-51 |
MsaG005244 | MsaG008069 | 0.811441 | 1.198949e-50 | 2.120807e-48 |
MsaG005244 | MsaG008368 | 0.803532 | 5.612035e-49 | 8.223609e-47 |
MsaG005244 | MsaG011443 | 0.800778 | 2.056041e-48 | 2.829202e-46 |
MsaG005244 | MsaG011586 | 0.804062 | 4.360615e-49 | 6.468996e-47 |
MsaG005244 | MsaG014725 | 0.830063 | 6.519316e-55 | 1.890118e-52 |
MsaG005244 | MsaG015813 | 0.800890 | 1.950664e-48 | 2.690929e-46 |
MsaG005244 | MsaG016078 | 0.808599 | 4.875846e-50 | 8.049737e-48 |
MsaG005244 | MsaG017088 | 0.839603 | 2.649526e-57 | 1.021415e-54 |
MsaG005244 | MsaG017473 | 0.806451 | 1.385532e-49 | 2.173351e-47 |
MsaG005244 | MsaG018212 | 0.827592 | 2.565818e-54 | 6.936763e-52 |
MsaG005244 | MsaG018317 | 0.817847 | 4.644015e-52 | 9.654949e-50 |
MsaG005244 | MsaG018430 | 0.821289 | 7.678439e-53 | 1.747372e-50 |
MsaG005244 | MsaG019159 | 0.808462 | 5.213044e-50 | 8.578327e-48 |
MsaG005244 | MsaG020062 | 0.827606 | 2.546059e-54 | 6.886058e-52 |
MsaG005244 | MsaG020645 | 0.810918 | 1.554410e-50 | 2.714567e-48 |
MsaG005244 | MsaG020787 | 0.812923 | 5.715538e-51 | 1.048752e-48 |
MsaG005244 | MsaG021423 | 0.804799 | 3.066503e-49 | 4.627341e-47 |
MsaG005244 | MsaG022116 | 0.805944 | 1.769725e-49 | 2.742736e-47 |
MsaG005244 | MsaG022494 | 0.807038 | 1.042952e-49 | 1.658672e-47 |
MsaG005244 | MsaG023396 | 0.817393 | 5.874141e-52 | 1.207051e-49 |
MsaG005244 | MsaG023680 | 0.800685 | 2.147684e-48 | 2.949149e-46 |
MsaG005244 | MsaG024036 | 0.809145 | 3.729495e-50 | 6.238972e-48 |
MsaG005244 | MsaG025722 | 0.823421 | 2.469214e-53 | 5.950063e-51 |
MsaG005244 | MsaG027027 | 0.810498 | 1.914381e-50 | 3.309178e-48 |
MsaG005244 | MsaG027749 | 0.823981 | 1.828753e-53 | 4.474057e-51 |
MsaG005244 | MsaG028350 | 0.805061 | 2.704963e-49 | 4.106756e-47 |
MsaG005244 | MsaG029132 | 0.811672 | 1.068526e-50 | 1.900833e-48 |
MsaG005244 | MsaG030267 | 0.805641 | 2.047427e-49 | 3.150702e-47 |
MsaG005244 | MsaG035439 | 0.801705 | 1.330869e-48 | 1.870122e-46 |
MsaG005244 | MsaG040666 | 0.808454 | 5.232619e-50 | 8.608828e-48 |
MsaG005244 | MsaG041926 | 0.807338 | 9.016820e-50 | 1.444237e-47 |
MsaG005244 | MsaG042142 | 0.821283 | 7.704406e-53 | 1.753007e-50 |
MsaG005244 | MsaG044614 | 0.816160 | 1.106994e-51 | 2.203824e-49 |
MsaG005244 | MsaG045113 | 0.820561 | 1.127313e-52 | 2.516416e-50 |
MsaG005244 | MsaG045873 | 0.820146 | 1.402153e-52 | 3.095858e-50 |
MsaG005244 | MsaG046470 | 0.814158 | 3.066538e-51 | 5.803556e-49 |
MsaG005244 | MsaG007871 | 0.821388 | 7.287944e-53 | 1.662834e-50 |
MsaG005244 | MsaG006602 | 0.807335 | 9.028019e-50 | 1.445938e-47 |
MsaG005244 | MsaG016152 | 0.820152 | 1.397461e-52 | 3.086021e-50 |
MsaG005244 | MsaG013115 | 0.800471 | 2.373402e-48 | 3.243287e-46 |
MsaG005244 | MsaG013071 | 0.801081 | 1.784338e-48 | 2.472031e-46 |
MsaG005244 | MsaG023443 | 0.809810 | 2.689819e-50 | 4.572441e-48 |
MsaG005244 | MsaG031626 | 0.805422 | 2.275516e-49 | 3.483694e-47 |
MsaG005244 | MsaG034520 | 0.809043 | 3.921350e-50 | 6.543798e-48 |
MsaG005244 | MsaG031808 | 0.802828 | 7.835832e-49 | 1.129674e-46 |
MsaG005244 | MsaG031699 | 0.833862 | 7.589872e-56 | 2.457268e-53 |
MsaG005244 | MsaG035918 | 0.801275 | 1.629082e-48 | 2.266870e-46 |
MsaG005244 | MsaG047040 | 0.810175 | 2.246078e-50 | 3.851947e-48 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG005244 | MtrunA17_Chr1g0205831 | 67.308 | 104 | 20 | 1 | 32 | 121 | 76 | 179 | 1.75e-40 | 132 |
MsaG005244 | MtrunA17_Chr1g0205831 | 60.294 | 68 | 27 | 0 | 31 | 98 | 4 | 71 | 2.71e-22 | 86.3 |
MsaG005244 | MtrunA17_Chr1g0205811 | 60.870 | 92 | 36 | 0 | 29 | 120 | 14 | 105 | 3.42e-35 | 117 |
MsaG005244 | MtrunA17_Chr1g0205801 | 62.366 | 93 | 35 | 0 | 25 | 117 | 8 | 100 | 2.03e-34 | 115 |
MsaG005244 | MtrunA17_Chr1g0205821 | 64.894 | 94 | 32 | 1 | 21 | 114 | 6 | 98 | 4.28e-34 | 116 |
MsaG005244 | MtrunA17_Chr1g0205821 | 63.736 | 91 | 28 | 1 | 21 | 111 | 91 | 176 | 1.02e-33 | 115 |
MsaG005244 | MtrunA17_Chr8g0364231 | 55.696 | 79 | 34 | 1 | 27 | 105 | 40 | 117 | 1.25e-21 | 89.0 |
MsaG005244 | MtrunA17_Chr3g0077911 | 48.837 | 86 | 41 | 1 | 29 | 114 | 8 | 90 | 1.05e-20 | 81.6 |
MsaG005244 | MtrunA17_Chr3g0077911 | 49.315 | 73 | 36 | 1 | 27 | 98 | 88 | 160 | 1.38e-13 | 63.2 |
MsaG005244 | MtrunA17_Chr5g0415031 | 47.500 | 80 | 42 | 0 | 27 | 106 | 41 | 120 | 1.38e-19 | 83.2 |
MsaG005244 | MtrunA17_Chr1g0206781 | 48.052 | 77 | 40 | 0 | 30 | 106 | 12 | 88 | 1.84e-19 | 77.4 |
MsaG005244 | MtrunA17_Chr3g0077881 | 47.872 | 94 | 43 | 2 | 27 | 114 | 6 | 99 | 4.03e-18 | 75.5 |
MsaG005244 | MtrunA17_Chr1g0205861 | 50.000 | 82 | 36 | 1 | 21 | 97 | 3 | 84 | 8.68e-18 | 73.2 |
MsaG005244 | MtrunA17_Chr4g0075861 | 48.837 | 86 | 44 | 0 | 29 | 114 | 89 | 174 | 2.17e-17 | 76.3 |
MsaG005244 | MtrunA17_Chr4g0075861 | 50.000 | 70 | 35 | 0 | 32 | 101 | 175 | 244 | 3.31e-16 | 73.2 |
MsaG005244 | MtrunA17_Chr4g0075861 | 44.211 | 95 | 47 | 3 | 25 | 117 | 8 | 98 | 1.23e-12 | 62.8 |
MsaG005244 | MtrunA17_Chr3g0077861 | 45.349 | 86 | 44 | 1 | 29 | 114 | 8 | 90 | 3.51e-17 | 72.8 |
MsaG005244 | MtrunA17_Chr3g0077861 | 48.101 | 79 | 38 | 2 | 27 | 102 | 88 | 166 | 6.87e-14 | 64.3 |
MsaG005244 | MtrunA17_Chr1g0206771 | 47.368 | 76 | 40 | 0 | 22 | 97 | 88 | 163 | 7.83e-17 | 72.4 |
MsaG005244 | MtrunA17_Chr1g0206771 | 44.000 | 75 | 42 | 0 | 27 | 101 | 14 | 88 | 2.03e-14 | 65.9 |
MsaG005244 | MtrunA17_Chr4g0075971 | 50.575 | 87 | 41 | 2 | 29 | 114 | 89 | 174 | 9.63e-17 | 74.7 |
MsaG005244 | MtrunA17_Chr4g0075971 | 50.000 | 70 | 35 | 0 | 32 | 101 | 175 | 244 | 5.85e-16 | 72.4 |
MsaG005244 | MtrunA17_Chr4g0075971 | 44.211 | 95 | 47 | 3 | 25 | 117 | 8 | 98 | 1.33e-12 | 63.2 |
MsaG005244 | MtrunA17_Chr6g0452001 | 46.154 | 78 | 42 | 0 | 27 | 104 | 61 | 138 | 3.17e-16 | 73.6 |
MsaG005244 | MtrunA17_Chr3g0077891 | 45.745 | 94 | 45 | 2 | 27 | 114 | 6 | 99 | 8.05e-16 | 69.3 |
MsaG005244 | MtrunA17_Chr3g0077891 | 44.737 | 76 | 41 | 1 | 29 | 103 | 99 | 174 | 1.49e-14 | 66.2 |
MsaG005244 | MtrunA17_Chr3g0077811 | 48.000 | 75 | 38 | 1 | 27 | 100 | 11 | 85 | 2.52e-15 | 67.0 |
MsaG005244 | MtrunA17_Chr3g0077941 | 47.312 | 93 | 44 | 2 | 27 | 114 | 6 | 98 | 3.42e-15 | 67.8 |
MsaG005244 | MtrunA17_Chr3g0077941 | 43.421 | 76 | 42 | 1 | 29 | 103 | 98 | 173 | 1.75e-12 | 60.8 |
MsaG005244 | MtrunA17_Chr6g0452021 | 42.529 | 87 | 47 | 2 | 29 | 114 | 43 | 127 | 5.61e-15 | 69.7 |
MsaG005244 | MtrunA17_Chr2g0328701 | 43.820 | 89 | 46 | 2 | 25 | 112 | 25 | 110 | 6.67e-14 | 63.2 |
MsaG005244 | MtrunA17_Chr8g0364021 | 40.964 | 83 | 47 | 1 | 28 | 110 | 43 | 123 | 2.19e-13 | 64.3 |
MsaG005244 | MtrunA17_Chr7g0218911 | 47.619 | 63 | 33 | 0 | 37 | 99 | 6 | 68 | 2.36e-12 | 59.3 |
MsaG005244 | MtrunA17_Chr1g0209201 | 50.000 | 60 | 29 | 1 | 37 | 96 | 24 | 82 | 5.39e-12 | 58.9 |
MsaG005244 | MtrunA17_Chr7g0218891 | 42.667 | 75 | 41 | 1 | 27 | 99 | 11 | 85 | 6.08e-12 | 60.5 |
MsaG005244 | MtrunA17_Chr7g0218891 | 35.556 | 90 | 53 | 1 | 15 | 104 | 178 | 262 | 6.94e-11 | 57.8 |
MsaG005244 | MtrunA17_Chr5g0431721 | 53.125 | 64 | 27 | 2 | 33 | 93 | 16 | 79 | 1.30e-11 | 57.0 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG005244 | AT1G24200.1 | 51.429 | 70 | 34 | 0 | 28 | 97 | 13 | 82 | 6.14e-18 | 75.9 |
MsaG005244 | AT1G24190.3 | 43.820 | 89 | 49 | 1 | 27 | 114 | 12 | 100 | 3.27e-17 | 76.6 |
MsaG005244 | AT1G24190.2 | 43.820 | 89 | 49 | 1 | 27 | 114 | 12 | 100 | 3.40e-17 | 76.6 |
MsaG005244 | AT1G24190.1 | 43.820 | 89 | 49 | 1 | 27 | 114 | 12 | 100 | 3.40e-17 | 76.6 |
MsaG005244 | AT1G70060.2 | 47.436 | 78 | 41 | 0 | 27 | 104 | 12 | 89 | 5.47e-17 | 75.9 |
MsaG005244 | AT1G70060.1 | 47.436 | 78 | 41 | 0 | 27 | 104 | 12 | 89 | 5.47e-17 | 75.9 |
MsaG005244 | AT3G01320.1 | 48.611 | 72 | 37 | 0 | 27 | 98 | 55 | 126 | 2.09e-16 | 74.3 |
MsaG005244 | AT3G01320.2 | 48.611 | 72 | 37 | 0 | 27 | 98 | 55 | 126 | 2.20e-16 | 74.3 |
MsaG005244 | AT1G10450.2 | 45.055 | 91 | 48 | 2 | 24 | 114 | 29 | 117 | 3.43e-16 | 73.6 |
MsaG005244 | AT1G10450.1 | 45.055 | 91 | 48 | 2 | 24 | 114 | 29 | 117 | 3.43e-16 | 73.6 |
MsaG005244 | AT1G10450.3 | 45.055 | 91 | 48 | 2 | 24 | 114 | 80 | 168 | 3.44e-16 | 73.6 |
MsaG005244 | AT5G15020.1 | 45.833 | 72 | 39 | 0 | 27 | 98 | 50 | 121 | 3.42e-15 | 70.9 |
MsaG005244 | AT5G15020.2 | 45.833 | 72 | 39 | 0 | 27 | 98 | 50 | 121 | 3.62e-15 | 70.9 |
MsaG005244 | AT1G59890.3 | 47.143 | 70 | 37 | 0 | 29 | 98 | 45 | 114 | 5.89e-15 | 70.1 |
MsaG005244 | AT1G59890.2 | 47.143 | 70 | 37 | 0 | 29 | 98 | 45 | 114 | 5.95e-15 | 70.1 |
MsaG005244 | AT1G59890.1 | 47.143 | 70 | 37 | 0 | 29 | 98 | 45 | 114 | 6.07e-15 | 70.1 |
MsaG005244 | AT1G59890.4 | 47.143 | 70 | 37 | 0 | 29 | 98 | 45 | 114 | 6.37e-15 | 70.1 |
MsaG005244 | AT5G35610.1 | 47.500 | 80 | 39 | 1 | 37 | 116 | 18 | 94 | 1.57e-14 | 65.9 |
MsaG005244 | AT5G15040.1 | 42.857 | 70 | 40 | 0 | 29 | 98 | 3 | 72 | 1.76e-13 | 61.6 |
MsaG005244 | AT5G15040.3 | 42.857 | 70 | 40 | 0 | 29 | 98 | 3 | 72 | 1.76e-13 | 61.6 |
MsaG005244 | AT5G15040.2 | 42.857 | 70 | 40 | 0 | 29 | 98 | 3 | 72 | 1.76e-13 | 61.6 |
MsaG005244 | AT1G27220.1 | 39.726 | 73 | 44 | 0 | 25 | 97 | 10 | 82 | 4.62e-13 | 62.8 |
MsaG005244 | AT1G70030.3 | 44.286 | 70 | 39 | 0 | 29 | 98 | 22 | 91 | 4.64e-13 | 61.6 |
MsaG005244 | AT1G70030.2 | 44.286 | 70 | 39 | 0 | 29 | 98 | 22 | 91 | 9.91e-13 | 60.8 |
MsaG005244 | AT1G70030.1 | 43.836 | 73 | 41 | 0 | 29 | 101 | 11 | 83 | 1.19e-12 | 61.2 |
MsaG005244 | AT1G23810.1 | 38.947 | 95 | 56 | 2 | 14 | 108 | 95 | 187 | 8.36e-12 | 60.1 |
MsaG005244 | AT1G24230.1 | 47.222 | 72 | 36 | 2 | 23 | 92 | 7 | 78 | 1.25e-11 | 59.7 |
MsaG005244 | AT1G24250.1 | 43.902 | 82 | 45 | 1 | 27 | 108 | 118 | 198 | 1.30e-11 | 59.7 |
MsaG005244 | AT1G27260.1 | 41.667 | 72 | 42 | 0 | 27 | 98 | 1 | 72 | 1.33e-11 | 59.3 |
MsaG005244 | AT5G15025.1 | 38.889 | 72 | 44 | 0 | 27 | 98 | 45 | 116 | 2.85e-11 | 56.6 |
MsaG005244 | AT1G24210.1 | 48.571 | 70 | 35 | 1 | 23 | 91 | 7 | 76 | 3.01e-11 | 57.4 |
MsaG005244 | AT1G27250.1 | 38.889 | 72 | 44 | 0 | 25 | 96 | 17 | 88 | 4.83e-11 | 56.6 |
Find 20 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
AACTCTTATATCAATTCTTT+TGG | 0.302742 | 1:-79406658 | None:intergenic |
AGAGCATCAAGATTTACTTT+TGG | 0.331136 | 1:+79406710 | MsaT005244.1:CDS |
AAGTTGCATTTCAAAATAAG+AGG | 0.445054 | 1:+79406592 | MsaT005244.1:CDS |
AGTTGTCAAAGAAGGAGTGA+TGG | 0.486303 | 1:+79406677 | MsaT005244.1:CDS |
TGAAATCTCATTTCCATTGG+AGG | 0.501735 | 1:+79406481 | MsaT005244.1:CDS |
TGAGTGCTGGTCCATCTTTG+AGG | 0.502051 | 1:-79406536 | None:intergenic |
ACATGAAATCTCATTTCCAT+TGG | 0.503318 | 1:+79406478 | None:intergenic |
CTCAACACTTTCTTGCCAGC+GGG | 0.511799 | 1:+79406735 | MsaT005244.1:CDS |
GATATAAGAGTTGTCAAAGA+AGG | 0.512296 | 1:+79406669 | MsaT005244.1:CDS |
ACACGAAATCTCACTTCCAT+TGG | 0.519367 | 1:+79406758 | MsaT005244.1:CDS |
AGGTGATAGATTAACATTCA+AGG | 0.536491 | 1:+79406797 | MsaT005244.1:CDS |
GATCAGTGCATCCTCAAAGA+TGG | 0.538557 | 1:+79406525 | MsaT005244.1:CDS |
AGTGAGATTTCGTGTCCCGC+TGG | 0.542973 | 1:-79406750 | None:intergenic |
ATTGCTTCATCTATGAGTGC+TGG | 0.548558 | 1:-79406549 | None:intergenic |
AGTTGCATTTCAAAATAAGA+GGG | 0.555199 | 1:+79406593 | MsaT005244.1:CDS |
TGTTGTTCATCACTATCCAA+TGG | 0.563918 | 1:-79406774 | None:intergenic |
GATGATCAACAACGATGTGA+TGG | 0.569542 | 1:+79406503 | MsaT005244.1:CDS |
CGTTGTTGATCATCCTCCAA+TGG | 0.597089 | 1:-79406494 | None:intergenic |
ACTCAACACTTTCTTGCCAG+CGG | 0.612834 | 1:+79406734 | MsaT005244.1:CDS |
TTGGATAGTGATGAACAACA+AGG | 0.741257 | 1:+79406777 | MsaT005244.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr1 | gene | 79406480 | 79406845 | 79406480 | ID=MsaG005244 |
Chr1 | mRNA | 79406480 | 79406845 | 79406480 | ID=MsaT005244.1;Parent=MsaG005244 |
Chr1 | exon | 79406480 | 79406845 | 79406480 | ID=MsaT005244.1.exon1;Parent=MsaT005244.1 |
Chr1 | CDS | 79406480 | 79406845 | 79406480 | ID=cds.MsaT005244.1;Parent=MsaT005244.1 |
Gene Sequence |
Protein sequence |