AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005247

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005247 MtrunA17_Chr1g0205831 67.308 104 20 1 32 121 76 179 1.75e-40 132
MsaG005247 MtrunA17_Chr1g0205831 60.294 68 27 0 31 98 4 71 2.71e-22 86.3
MsaG005247 MtrunA17_Chr1g0205811 60.870 92 36 0 29 120 14 105 3.42e-35 117
MsaG005247 MtrunA17_Chr1g0205801 62.366 93 35 0 25 117 8 100 2.03e-34 115
MsaG005247 MtrunA17_Chr1g0205821 64.894 94 32 1 21 114 6 98 4.28e-34 116
MsaG005247 MtrunA17_Chr1g0205821 63.736 91 28 1 21 111 91 176 1.02e-33 115
MsaG005247 MtrunA17_Chr8g0364231 55.696 79 34 1 27 105 40 117 1.25e-21 89.0
MsaG005247 MtrunA17_Chr3g0077911 48.837 86 41 1 29 114 8 90 1.05e-20 81.6
MsaG005247 MtrunA17_Chr3g0077911 49.315 73 36 1 27 98 88 160 1.38e-13 63.2
MsaG005247 MtrunA17_Chr5g0415031 47.500 80 42 0 27 106 41 120 1.38e-19 83.2
MsaG005247 MtrunA17_Chr1g0206781 48.052 77 40 0 30 106 12 88 1.84e-19 77.4
MsaG005247 MtrunA17_Chr3g0077881 47.872 94 43 2 27 114 6 99 4.03e-18 75.5
MsaG005247 MtrunA17_Chr1g0205861 50.000 82 36 1 21 97 3 84 8.68e-18 73.2
MsaG005247 MtrunA17_Chr4g0075861 48.837 86 44 0 29 114 89 174 2.17e-17 76.3
MsaG005247 MtrunA17_Chr4g0075861 50.000 70 35 0 32 101 175 244 3.31e-16 73.2
MsaG005247 MtrunA17_Chr4g0075861 44.211 95 47 3 25 117 8 98 1.23e-12 62.8
MsaG005247 MtrunA17_Chr3g0077861 45.349 86 44 1 29 114 8 90 3.51e-17 72.8
MsaG005247 MtrunA17_Chr3g0077861 48.101 79 38 2 27 102 88 166 6.87e-14 64.3
MsaG005247 MtrunA17_Chr1g0206771 47.368 76 40 0 22 97 88 163 7.83e-17 72.4
MsaG005247 MtrunA17_Chr1g0206771 44.000 75 42 0 27 101 14 88 2.03e-14 65.9
MsaG005247 MtrunA17_Chr4g0075971 50.575 87 41 2 29 114 89 174 9.63e-17 74.7
MsaG005247 MtrunA17_Chr4g0075971 50.000 70 35 0 32 101 175 244 5.85e-16 72.4
MsaG005247 MtrunA17_Chr4g0075971 44.211 95 47 3 25 117 8 98 1.33e-12 63.2
MsaG005247 MtrunA17_Chr6g0452001 46.154 78 42 0 27 104 61 138 3.17e-16 73.6
MsaG005247 MtrunA17_Chr3g0077891 45.745 94 45 2 27 114 6 99 8.05e-16 69.3
MsaG005247 MtrunA17_Chr3g0077891 44.737 76 41 1 29 103 99 174 1.49e-14 66.2
MsaG005247 MtrunA17_Chr3g0077811 48.000 75 38 1 27 100 11 85 2.52e-15 67.0
MsaG005247 MtrunA17_Chr3g0077941 47.312 93 44 2 27 114 6 98 3.42e-15 67.8
MsaG005247 MtrunA17_Chr3g0077941 43.421 76 42 1 29 103 98 173 1.75e-12 60.8
MsaG005247 MtrunA17_Chr6g0452021 42.529 87 47 2 29 114 43 127 5.61e-15 69.7
MsaG005247 MtrunA17_Chr2g0328701 43.820 89 46 2 25 112 25 110 6.67e-14 63.2
MsaG005247 MtrunA17_Chr8g0364021 40.964 83 47 1 28 110 43 123 2.19e-13 64.3
MsaG005247 MtrunA17_Chr7g0218911 47.619 63 33 0 37 99 6 68 2.36e-12 59.3
MsaG005247 MtrunA17_Chr1g0209201 50.000 60 29 1 37 96 24 82 5.39e-12 58.9
MsaG005247 MtrunA17_Chr7g0218891 42.667 75 41 1 27 99 11 85 6.08e-12 60.5
MsaG005247 MtrunA17_Chr7g0218891 35.556 90 53 1 15 104 178 262 6.94e-11 57.8
MsaG005247 MtrunA17_Chr5g0431721 53.125 64 27 2 33 93 16 79 1.30e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005247 AT1G24200.1 51.429 70 34 0 28 97 13 82 6.14e-18 75.9
MsaG005247 AT1G24190.3 43.820 89 49 1 27 114 12 100 3.27e-17 76.6
MsaG005247 AT1G24190.2 43.820 89 49 1 27 114 12 100 3.40e-17 76.6
MsaG005247 AT1G24190.1 43.820 89 49 1 27 114 12 100 3.40e-17 76.6
MsaG005247 AT1G70060.2 47.436 78 41 0 27 104 12 89 5.47e-17 75.9
MsaG005247 AT1G70060.1 47.436 78 41 0 27 104 12 89 5.47e-17 75.9
MsaG005247 AT3G01320.1 48.611 72 37 0 27 98 55 126 2.09e-16 74.3
MsaG005247 AT3G01320.2 48.611 72 37 0 27 98 55 126 2.20e-16 74.3
MsaG005247 AT1G10450.2 45.055 91 48 2 24 114 29 117 3.43e-16 73.6
MsaG005247 AT1G10450.1 45.055 91 48 2 24 114 29 117 3.43e-16 73.6
MsaG005247 AT1G10450.3 45.055 91 48 2 24 114 80 168 3.44e-16 73.6
MsaG005247 AT5G15020.1 45.833 72 39 0 27 98 50 121 3.42e-15 70.9
MsaG005247 AT5G15020.2 45.833 72 39 0 27 98 50 121 3.62e-15 70.9
MsaG005247 AT1G59890.3 47.143 70 37 0 29 98 45 114 5.89e-15 70.1
MsaG005247 AT1G59890.2 47.143 70 37 0 29 98 45 114 5.95e-15 70.1
MsaG005247 AT1G59890.1 47.143 70 37 0 29 98 45 114 6.07e-15 70.1
MsaG005247 AT1G59890.4 47.143 70 37 0 29 98 45 114 6.37e-15 70.1
MsaG005247 AT5G35610.1 47.500 80 39 1 37 116 18 94 1.57e-14 65.9
MsaG005247 AT5G15040.1 42.857 70 40 0 29 98 3 72 1.76e-13 61.6
MsaG005247 AT5G15040.3 42.857 70 40 0 29 98 3 72 1.76e-13 61.6
MsaG005247 AT5G15040.2 42.857 70 40 0 29 98 3 72 1.76e-13 61.6
MsaG005247 AT1G27220.1 39.726 73 44 0 25 97 10 82 4.62e-13 62.8
MsaG005247 AT1G70030.3 44.286 70 39 0 29 98 22 91 4.64e-13 61.6
MsaG005247 AT1G70030.2 44.286 70 39 0 29 98 22 91 9.91e-13 60.8
MsaG005247 AT1G70030.1 43.836 73 41 0 29 101 11 83 1.19e-12 61.2
MsaG005247 AT1G23810.1 38.947 95 56 2 14 108 95 187 8.36e-12 60.1
MsaG005247 AT1G24230.1 47.222 72 36 2 23 92 7 78 1.25e-11 59.7
MsaG005247 AT1G24250.1 43.902 82 45 1 27 108 118 198 1.30e-11 59.7
MsaG005247 AT1G27260.1 41.667 72 42 0 27 98 1 72 1.33e-11 59.3
MsaG005247 AT5G15025.1 38.889 72 44 0 27 98 45 116 2.85e-11 56.6
MsaG005247 AT1G24210.1 48.571 70 35 1 23 91 7 76 3.01e-11 57.4
MsaG005247 AT1G27250.1 38.889 72 44 0 25 96 17 88 4.83e-11 56.6

Find 20 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCTTATATCAATTCTTT+TGG 0.302742 1:-79423957 None:intergenic
AGAGCATCAAGATTTACTTT+TGG 0.331136 1:+79424009 MsaT005247.1:CDS
AAGTTGCATTTCAAAATAAG+AGG 0.445054 1:+79423891 MsaT005247.1:CDS
AGTTGTCAAAGAAGGAGTGA+TGG 0.486303 1:+79423976 MsaT005247.1:CDS
TGAAATCTCATTTCCATTGG+AGG 0.501735 1:+79423780 MsaT005247.1:CDS
TGAGTGCTGGTCCATCTTTG+AGG 0.502051 1:-79423835 None:intergenic
ACATGAAATCTCATTTCCAT+TGG 0.503318 1:+79423777 None:intergenic
CTCAACACTTTCTTGCCAGC+GGG 0.511799 1:+79424034 MsaT005247.1:CDS
GATATAAGAGTTGTCAAAGA+AGG 0.512296 1:+79423968 MsaT005247.1:CDS
ACACGAAATCTCACTTCCAT+TGG 0.519367 1:+79424057 MsaT005247.1:CDS
AGGTGATAGATTAACATTCA+AGG 0.536491 1:+79424096 MsaT005247.1:CDS
GATCAGTGCATCCTCAAAGA+TGG 0.538557 1:+79423824 MsaT005247.1:CDS
AGTGAGATTTCGTGTCCCGC+TGG 0.542973 1:-79424049 None:intergenic
ATTGCTTCATCTATGAGTGC+TGG 0.548558 1:-79423848 None:intergenic
AGTTGCATTTCAAAATAAGA+GGG 0.555199 1:+79423892 MsaT005247.1:CDS
TGTTGTTCATCACTATCCAA+TGG 0.563918 1:-79424073 None:intergenic
GATGATCAACAACGATGTGA+TGG 0.569542 1:+79423802 MsaT005247.1:CDS
CGTTGTTGATCATCCTCCAA+TGG 0.597089 1:-79423793 None:intergenic
ACTCAACACTTTCTTGCCAG+CGG 0.612834 1:+79424033 MsaT005247.1:CDS
TTGGATAGTGATGAACAACA+AGG 0.741257 1:+79424076 MsaT005247.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 79423779 79424144 79423779 ID=MsaG005247
Chr1 mRNA 79423779 79424144 79423779 ID=MsaT005247.1;Parent=MsaG005247
Chr1 exon 79423779 79424144 79423779 ID=MsaT005247.1.exon1;Parent=MsaT005247.1
Chr1 CDS 79423779 79424144 79423779 ID=cds.MsaT005247.1;Parent=MsaT005247.1
Gene Sequence

>MsaT005247.1

ATGAAATCTCATTTCCATTGGAGGATGATCAACAACGATGTGATGGATCAGTGCATCCTCAAAGATGGACCAGCACTCATAGATGAAGCAATGTCGCTTGTAACAGCAGTTAAAGTTGCATTTCAAAATAAGAGGGAAAAGTATGATGAATTCTTGAAACTCCTAGTTGATTACAATGCCAAAAGAATTGATATAAGAGTTGTCAAAGAAGGAGTGATGGAGTTGTTTAAAGAGCATCAAGATTTACTTTTGGGACTCAACACTTTCTTGCCAGCGGGACACGAAATCTCACTTCCATTGGATAGTGATGAACAACAAGGTGATAGATTAACATTCAAGGATGAACAGAAAGATGATGAGCAATAA
Protein sequence

>MsaT005247.1

MKSHFHWRMINNDVMDQCILKDGPALIDEAMSLVTAVKVAFQNKREKYDEFLKLLVDYNAKRIDIRVVKEGVMELFKEHQDLLLGLNTFLPAGHEISLPLDSDEQQGDRLTFKDEQKDDEQ*