AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005248


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005248 MtrunA17_Chr1g0205821 86.441 177 24 0 1 177 1 177 4.94e-107 308
MsaG005248 MtrunA17_Chr1g0205821 72.892 166 45 0 94 259 8 173 5.12e-80 239
MsaG005248 MtrunA17_Chr1g0205821 53.608 97 44 1 175 271 3 98 1.48e-24 97.1
MsaG005248 MtrunA17_Chr1g0205831 64.198 162 43 1 17 178 6 152 1.51e-64 200
MsaG005248 MtrunA17_Chr1g0205831 55.440 193 58 2 99 278 2 179 1.39e-62 195
MsaG005248 MtrunA17_Chr1g0205831 62.338 77 29 0 16 92 76 152 3.19e-23 93.6
MsaG005248 MtrunA17_Chr4g0075861 43.580 257 132 4 1 257 1 244 4.35e-57 187
MsaG005248 MtrunA17_Chr4g0075861 42.515 167 87 2 96 262 9 166 8.97e-32 120
MsaG005248 MtrunA17_Chr4g0075971 43.411 258 131 6 1 257 1 244 1.10e-52 181
MsaG005248 MtrunA17_Chr4g0075971 42.857 168 85 4 96 262 9 166 3.24e-29 116
MsaG005248 MtrunA17_Chr7g0218891 31.203 266 173 5 1 260 1 262 8.44e-36 129
MsaG005248 MtrunA17_Chr1g0206771 37.647 170 99 1 84 253 1 163 3.18e-35 125
MsaG005248 MtrunA17_Chr1g0206771 40.964 166 91 1 2 167 5 163 2.34e-34 122
MsaG005248 MtrunA17_Chr3g0077911 48.276 145 73 2 21 164 13 156 4.58e-34 121
MsaG005248 MtrunA17_Chr3g0077911 42.038 157 86 3 99 254 8 160 8.18e-31 112
MsaG005248 MtrunA17_Chr3g0077911 44.318 88 45 2 185 272 8 91 3.28e-14 68.9
MsaG005248 MtrunA17_Chr3g0077891 43.195 169 90 3 11 173 6 174 6.69e-33 119
MsaG005248 MtrunA17_Chr3g0077891 38.922 167 96 3 99 259 8 174 1.52e-27 104
MsaG005248 MtrunA17_Chr1g0205801 61.702 94 36 0 6 99 5 98 8.42e-32 114
MsaG005248 MtrunA17_Chr1g0205801 56.322 87 38 0 99 185 12 98 2.85e-25 97.1
MsaG005248 MtrunA17_Chr1g0205801 52.222 90 42 1 185 274 12 100 8.30e-23 90.5
MsaG005248 MtrunA17_Chr3g0077941 42.941 170 88 4 11 173 6 173 8.66e-32 115
MsaG005248 MtrunA17_Chr3g0077941 38.554 166 97 3 99 259 8 173 1.32e-25 99.8
MsaG005248 MtrunA17_Chr3g0077861 46.853 143 74 2 23 164 15 156 1.25e-31 115
MsaG005248 MtrunA17_Chr3g0077861 39.490 157 90 3 99 254 8 160 1.73e-27 104
MsaG005248 MtrunA17_Chr3g0077861 49.315 73 36 1 11 82 88 160 2.54e-11 60.8
MsaG005248 MtrunA17_Chr5g0415031 40.260 154 92 0 10 163 40 193 4.68e-31 122
MsaG005248 MtrunA17_Chr5g0415031 36.184 152 97 0 98 249 42 193 6.76e-29 115
MsaG005248 MtrunA17_Chr5g0415031 42.697 89 51 0 184 272 42 130 3.66e-16 78.6
MsaG005248 MtrunA17_Chr3g0077881 42.771 166 88 4 11 170 6 170 5.72e-31 114
MsaG005248 MtrunA17_Chr3g0077881 40.244 164 91 4 99 256 8 170 1.40e-27 105
MsaG005248 MtrunA17_Chr1g0205811 57.000 100 42 1 1 99 1 100 5.55e-30 109
MsaG005248 MtrunA17_Chr1g0205811 53.684 95 43 1 183 277 12 105 2.62e-26 100
MsaG005248 MtrunA17_Chr1g0205811 52.809 89 42 0 97 185 12 100 3.45e-26 99.8
MsaG005248 MtrunA17_Chr8g0364231 40.909 154 89 2 10 162 39 191 1.28e-27 112
MsaG005248 MtrunA17_Chr8g0364231 36.424 151 96 0 98 248 41 191 1.27e-25 106
MsaG005248 MtrunA17_Chr8g0364231 47.436 78 40 1 184 261 41 117 6.28e-16 77.8
MsaG005248 MtrunA17_Chr6g0452021 37.748 151 92 2 13 163 43 191 2.84e-24 101
MsaG005248 MtrunA17_Chr6g0452021 35.099 151 96 2 99 249 43 191 1.81e-23 99.4
MsaG005248 MtrunA17_Chr6g0452001 36.364 154 98 0 10 163 60 213 1.23e-23 100
MsaG005248 MtrunA17_Chr6g0452001 32.895 152 102 0 98 249 62 213 1.26e-21 94.7
MsaG005248 MtrunA17_Chr6g0452001 37.079 89 56 0 184 272 62 150 7.31e-12 65.9
MsaG005248 MtrunA17_Chr1g0209201 40.625 128 66 3 7 134 10 127 4.59e-20 84.3
MsaG005248 MtrunA17_Chr1g0209201 36.842 133 74 3 88 220 5 127 1.04e-17 77.8
MsaG005248 MtrunA17_Chr1g0209201 34.483 87 52 2 166 252 1 82 9.72e-11 58.9
MsaG005248 MtrunA17_Chr1g0206781 36.364 121 51 1 100 220 12 106 7.56e-19 80.1
MsaG005248 MtrunA17_Chr1g0206781 46.250 80 43 0 186 265 12 91 1.28e-18 79.7
MsaG005248 MtrunA17_Chr1g0206781 37.190 121 50 1 14 134 12 106 6.28e-17 75.1
MsaG005248 MtrunA17_Chr2g0328701 41.284 109 55 2 96 204 26 125 2.24e-17 76.6
MsaG005248 MtrunA17_Chr2g0328701 42.017 119 57 4 1 118 18 125 1.22e-15 71.6
MsaG005248 MtrunA17_Chr2g0328701 36.782 87 52 1 181 267 25 108 3.31e-11 59.7
MsaG005248 MtrunA17_Chr1g0205861 50.000 70 35 0 98 167 15 84 1.52e-15 71.2
MsaG005248 MtrunA17_Chr1g0205861 42.353 85 46 1 184 268 15 96 5.70e-14 67.0
MsaG005248 MtrunA17_Chr1g0205861 48.611 72 37 0 10 81 13 84 8.73e-14 66.6
MsaG005248 MtrunA17_Chr8g0364021 39.583 96 56 1 89 184 34 127 1.74e-14 71.2
MsaG005248 MtrunA17_Chr8g0364021 41.758 91 51 1 12 102 43 131 1.40e-13 68.6
MsaG005248 MtrunA17_Chr8g0364021 33.607 122 73 2 141 262 6 119 2.90e-13 67.8
MsaG005248 MtrunA17_Chr3g0077811 51.724 58 28 0 27 84 28 85 3.85e-12 62.0
MsaG005248 MtrunA17_Chr7g0256011 46.269 67 36 0 11 77 14 80 4.10e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005248 AT1G24230.1 30.400 250 146 7 10 251 11 240 1.49e-27 107
MsaG005248 AT1G24230.1 36.196 163 88 2 98 259 13 160 1.90e-25 101
MsaG005248 AT1G27260.1 38.889 180 102 3 98 272 2 178 4.44e-26 102
MsaG005248 AT1G27260.1 35.577 208 129 3 11 214 1 207 3.44e-24 97.8
MsaG005248 AT1G70060.2 39.610 154 92 1 10 162 11 164 3.30e-25 105
MsaG005248 AT1G70060.2 34.868 152 98 1 98 248 13 164 1.23e-22 98.2
MsaG005248 AT1G70060.2 45.070 71 39 0 184 254 13 83 2.59e-12 67.4
MsaG005248 AT1G70060.1 39.610 154 92 1 10 162 11 164 3.30e-25 105
MsaG005248 AT1G70060.1 34.868 152 98 1 98 248 13 164 1.23e-22 98.2
MsaG005248 AT1G70060.1 45.070 71 39 0 184 254 13 83 2.59e-12 67.4
MsaG005248 AT5G15020.2 38.065 155 92 2 10 163 49 200 6.37e-25 104
MsaG005248 AT5G15020.2 34.641 153 96 2 98 249 51 200 5.56e-23 99.0
MsaG005248 AT5G15020.2 40.845 71 42 0 184 254 51 121 4.79e-11 63.5
MsaG005248 AT5G15020.1 38.065 155 92 2 10 163 49 200 7.72e-25 104
MsaG005248 AT5G15020.1 34.641 153 96 2 98 249 51 200 6.92e-23 98.6
MsaG005248 AT5G15020.1 40.845 71 42 0 184 254 51 121 5.36e-11 63.5
MsaG005248 AT3G01320.1 36.875 160 98 2 4 161 46 204 1.31e-23 100
MsaG005248 AT3G01320.1 34.810 158 98 2 98 255 56 208 2.53e-22 97.1
MsaG005248 AT3G01320.2 36.875 160 98 2 4 161 46 204 1.36e-23 100
MsaG005248 AT3G01320.2 34.810 158 98 2 98 255 56 208 2.57e-22 97.1
MsaG005248 AT1G24190.2 37.349 166 93 3 10 169 11 171 2.32e-23 100
MsaG005248 AT1G24190.2 32.075 159 107 1 98 255 13 171 1.56e-21 94.7
MsaG005248 AT1G24190.2 43.421 76 42 1 184 258 13 88 2.30e-12 67.8
MsaG005248 AT1G24190.1 37.349 166 93 3 10 169 11 171 2.34e-23 100
MsaG005248 AT1G24190.1 32.075 159 107 1 98 255 13 171 1.65e-21 94.7
MsaG005248 AT1G24190.1 43.421 76 42 1 184 258 13 88 2.36e-12 67.4
MsaG005248 AT1G24190.3 37.349 166 93 3 10 169 11 171 2.35e-23 100
MsaG005248 AT1G24190.3 32.075 159 107 1 98 255 13 171 1.59e-21 94.7
MsaG005248 AT1G24190.3 43.421 76 42 1 184 258 13 88 2.26e-12 67.8
MsaG005248 AT1G24200.1 35.065 154 95 2 95 247 10 159 4.68e-23 94.0
MsaG005248 AT1G24200.1 37.013 154 92 2 9 161 10 159 5.12e-22 91.3
MsaG005248 AT1G24200.1 41.429 70 41 0 184 253 13 82 5.75e-12 63.9
MsaG005248 AT1G24250.1 29.864 221 134 4 11 211 12 231 7.65e-23 94.7
MsaG005248 AT1G24250.1 33.166 199 110 6 94 272 10 205 2.37e-20 88.2
MsaG005248 AT1G24250.1 46.377 69 37 0 6 74 113 181 9.66e-11 61.2
MsaG005248 AT1G10450.2 37.255 153 94 2 95 247 29 179 1.65e-22 97.4
MsaG005248 AT1G10450.2 34.031 191 109 4 9 184 29 217 1.85e-21 94.4
MsaG005248 AT1G10450.2 45.714 70 38 0 185 254 33 102 4.75e-12 66.6
MsaG005248 AT1G10450.1 37.255 153 94 2 95 247 29 179 1.65e-22 97.4
MsaG005248 AT1G10450.1 34.031 191 109 4 9 184 29 217 1.85e-21 94.4
MsaG005248 AT1G10450.1 45.714 70 38 0 185 254 33 102 4.75e-12 66.6
MsaG005248 AT1G10450.3 37.255 153 94 2 95 247 80 230 1.67e-22 97.4
MsaG005248 AT1G10450.3 34.031 191 109 4 9 184 80 268 1.55e-21 94.7
MsaG005248 AT1G10450.3 45.714 70 38 0 185 254 84 153 5.20e-12 66.6
MsaG005248 AT1G23810.1 29.224 219 145 4 2 211 3 220 2.56e-22 93.2
MsaG005248 AT1G23810.1 34.574 188 111 5 94 272 10 194 2.62e-21 90.5
MsaG005248 AT1G70030.1 37.584 149 91 2 13 161 11 157 3.81e-21 87.8
MsaG005248 AT1G70030.1 35.570 149 94 2 99 247 11 157 6.37e-20 84.7
MsaG005248 AT1G59890.2 35.329 167 103 3 13 178 45 207 6.93e-21 92.8
MsaG005248 AT1G59890.2 33.333 153 94 2 99 248 45 192 1.58e-18 85.9
MsaG005248 AT1G59890.2 45.714 70 38 0 185 254 45 114 4.37e-12 66.6
MsaG005248 AT1G59890.4 35.329 167 103 3 13 178 45 207 7.04e-21 92.8
MsaG005248 AT1G59890.4 33.333 153 94 2 99 248 45 192 1.53e-18 85.9
MsaG005248 AT1G59890.4 45.714 70 38 0 185 254 45 114 4.35e-12 66.6
MsaG005248 AT1G59890.1 35.329 167 103 3 13 178 45 207 7.26e-21 92.8
MsaG005248 AT1G59890.1 33.333 153 94 2 99 248 45 192 1.60e-18 85.9
MsaG005248 AT1G59890.1 45.714 70 38 0 185 254 45 114 4.44e-12 66.6
MsaG005248 AT1G59890.3 35.329 167 103 3 13 178 45 207 7.39e-21 92.8
MsaG005248 AT1G59890.3 33.333 153 94 2 99 248 45 192 1.60e-18 85.9
MsaG005248 AT1G59890.3 45.714 70 38 0 185 254 45 114 4.44e-12 66.6
MsaG005248 AT1G27280.1 32.000 200 127 5 21 214 1 197 1.90e-18 82.4
MsaG005248 AT1G27280.1 33.571 140 91 1 110 247 4 143 1.06e-13 69.3
MsaG005248 AT1G27270.1 34.568 162 93 2 98 246 13 174 2.40e-15 73.9
MsaG005248 AT1G27270.1 35.802 162 91 3 12 160 13 174 4.14e-15 73.6
MsaG005248 AT1G70030.2 38.136 118 70 2 99 214 22 138 5.96e-15 70.9
MsaG005248 AT1G70030.2 40.000 115 63 2 13 122 22 135 4.84e-14 68.2
MsaG005248 AT1G27220.1 33.945 109 65 1 9 117 10 111 1.35e-14 70.9
MsaG005248 AT5G15040.1 44.156 77 41 1 99 175 3 77 7.20e-14 66.2
MsaG005248 AT5G15040.1 42.857 77 42 1 13 89 3 77 3.64e-13 64.3
MsaG005248 AT5G15040.1 42.857 70 40 0 185 254 3 72 2.61e-12 62.0
MsaG005248 AT5G15040.3 44.156 77 41 1 99 175 3 77 7.20e-14 66.2
MsaG005248 AT5G15040.3 42.857 77 42 1 13 89 3 77 3.64e-13 64.3
MsaG005248 AT5G15040.3 42.857 70 40 0 185 254 3 72 2.61e-12 62.0
MsaG005248 AT5G15040.2 44.156 77 41 1 99 175 3 77 7.20e-14 66.2
MsaG005248 AT5G15040.2 42.857 77 42 1 13 89 3 77 3.64e-13 64.3
MsaG005248 AT5G15040.2 42.857 70 40 0 185 254 3 72 2.61e-12 62.0
MsaG005248 AT1G70030.3 42.268 97 55 1 13 109 22 117 1.16e-13 66.6
MsaG005248 AT1G70030.3 40.206 97 57 1 99 195 22 117 1.24e-13 66.6
MsaG005248 AT1G24210.1 45.333 75 39 1 4 76 3 77 6.07e-13 65.5
MsaG005248 AT5G15025.1 40.741 81 48 0 2 82 36 116 1.04e-12 63.9
MsaG005248 AT1G27250.1 44.928 69 38 0 12 80 20 88 1.01e-11 61.6

Find 43 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCTTACATCAAGTCTTT+TGG 0.279157 1:-79426940 None:intergenic
AACTCTTACATCAATTCTTT+TGG 0.285232 1:-79426682 None:intergenic
GACTCTTCTATCAATTCTTT+TGG 0.310378 1:-79426424 None:intergenic
GTGAAACTCCTACAAGATTT+CGG 0.391833 1:+79426399 MsaT005248.1:CDS
GGTTACATTTGAAGATAAGA+TGG 0.406022 1:+79426617 MsaT005248.1:CDS
GGTTACATTTAAAGATAAGA+TGG 0.406022 1:+79426875 MsaT005248.1:CDS
AGTTATGAAAGAAGGAATGA+TGG 0.450472 1:+79426959 MsaT005248.1:CDS
CGATGCAAGGGCGTTTGTAA+AGG 0.450885 1:+79426329 MsaT005248.1:CDS
ATGCTGTGTTTCAAGATAAG+AGG 0.464716 1:+79426358 MsaT005248.1:CDS
ATGAACAGCTCAAGAAGAAC+AGG 0.465556 1:+79426294 MsaT005248.1:CDS
AGTCGTCAAAGAAGGAGTTA+TGG 0.477577 1:+79426443 MsaT005248.1:CDS
AGTTGTCAAAGAAGGAATGA+TGG 0.479247 1:+79426701 MsaT005248.1:CDS
GGCAAGAAGATGTTGAATCT+TGG 0.485703 1:-79426495 None:intergenic
GGCAAGAAGGTGTTGAATCC+CGG 0.486543 1:-79426753 None:intergenic
ACATGAAATCTCACTTCCAT+TGG 0.500530 1:+79426524 MsaT005248.1:CDS
ACACAAAATCTCAATTCCAT+TGG 0.505572 1:+79426782 MsaT005248.1:CDS
GATAGAAGAGTCGTCAAAGA+AGG 0.515587 1:+79426435 MsaT005248.1:CDS
TTCAACATCTTCTTGCCACC+AGG 0.516818 1:+79426501 MsaT005248.1:CDS
ACACAAAATCTCACTTCCAT+TGG 0.521284 1:+79427040 MsaT005248.1:CDS
CGCCCTTGCATCGTCAATCA+CGG 0.521774 1:-79426319 None:intergenic
TGCTGTGTTTCAAGATAAGA+GGG 0.528588 1:+79426359 MsaT005248.1:CDS
GATGTAAGAGTTGTCAAAGA+AGG 0.540360 1:+79426693 MsaT005248.1:CDS
GATGTAAGAGTTATGAAAGA+AGG 0.540360 1:+79426951 MsaT005248.1:CDS
AGATAAGATGGAAATGTTCG+AGG 0.540381 1:+79426887 MsaT005248.1:CDS
GTCCCGTGATTGACGATGCA+AGG 0.545886 1:+79426316 MsaT005248.1:CDS
GATGAACAACAAGATGAAGA+TGG 0.553215 1:+79427068 MsaT005248.1:CDS
TGTTGTTCATCACCATCCAA+TGG 0.554632 1:-79427056 None:intergenic
TTTGTGTCCCTCTGGCAAGA+AGG 0.563533 1:-79426766 None:intergenic
TTTGTGTCCCTCTGGCAAGA+AGG 0.563533 1:-79427024 None:intergenic
GCCCTTGCATCGTCAATCAC+GGG 0.577829 1:-79426318 None:intergenic
TCCCGTGATTGACGATGCAA+GGG 0.580973 1:+79426317 MsaT005248.1:CDS
TTCAACACCTTCTTGCCAGA+GGG 0.592249 1:+79426759 MsaT005248.1:CDS
TTCAACACCTTCTTGCCAGA+GGG 0.592249 1:+79427017 MsaT005248.1:CDS
AAAATCTCACTTCCATTGGA+TGG 0.592611 1:+79427044 MsaT005248.1:CDS
TGTTGTAAATCATCATCCAA+TGG 0.600709 1:-79426798 None:intergenic
ACCGCTCGAAGATGAAGCAA+TGG 0.613500 1:+79426575 MsaT005248.1:CDS
GAGTTGAAAGATGCACAGAA+AGG 0.613537 1:+79427095 MsaT005248.1:CDS
GGAAGTGAGATTTCATGTCC+TGG 0.614261 1:-79426519 None:intergenic
ACCATTGCTTCATCTTCGAG+CGG 0.637751 1:-79426576 None:intergenic
TGTTGTAGATCATCATCCAA+TGG 0.641812 1:-79426540 None:intergenic
ATTCAACACCTTCTTGCCAG+AGG 0.655191 1:+79426758 MsaT005248.1:CDS
ATTCAACACCTTCTTGCCAG+AGG 0.655191 1:+79427016 MsaT005248.1:CDS
AGTGAGATTTCATGTCCTGG+TGG 0.759321 1:-79426516 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 79426294 79427130 79426294 ID=MsaG005248
Chr1 mRNA 79426294 79427130 79426294 ID=MsaT005248.1;Parent=MsaG005248
Chr1 exon 79426294 79427130 79426294 ID=MsaT005248.1.exon1;Parent=MsaT005248.1
Chr1 CDS 79426294 79427130 79426294 ID=cds.MsaT005248.1;Parent=MsaT005248.1
Gene Sequence

>MsaT005248.1

ATGAACAGCTCAAGAAGAACAGGTCCCGTGATTGACGATGCAAGGGCGTTTGTAAAGGACGTAGATGCTGTGTTTCAAGATAAGAGGGAAAAGTATGATGAATTCGTGAAACTCCTACAAGATTTCGGCGCCAAAAGAATTGATAGAAGAGTCGTCAAAGAAGGAGTTATGGAGTTGTTTAAAGATCATCAAGATTTACTTCCAAGATTCAACATCTTCTTGCCACCAGGACATGAAATCTCACTTCCATTGGATGATGATCTACAACAACATGATCGATCACCGCTCGAAGATGAAGCAATGGTGTTTTTAACAGCAGTTAAGGTTACATTTGAAGATAAGATGGAAAAGTTCAATGAATTCTTGAAACTCCTAGCTGATTACCGTGCCAAAAGAATTGATGTAAGAGTTGTCAAAGAAGGAATGATGGAGTTGTTTAAAGACCATAAAAATTTACTTCCGGGATTCAACACCTTCTTGCCAGAGGGACACAAAATCTCAATTCCATTGGATGATGATTTACAACAACGTGATCGATCAGCACTCGAAGATGAAGCAACAGCATTTTTAACAGCAGTTAAGGTTACATTTAAAGATAAGATGGAAATGTTCGAGGAATTCTTGAAACTCCTAGCTGATTACCATGCCAAAAGACTTGATGTAAGAGTTATGAAAGAAGGAATGATGGAGTTGTTTAAAGACCATAAAAATTTACTTTTGGGATTCAACACCTTCTTGCCAGAGGGACACAAAATCTCACTTCCATTGGATGGTGATGAACAACAAGATGAAGATGGATCAGAGTTGAAAGATGCACAGAAAGGTGATGAACAATAA

Protein sequence

>MsaT005248.1

MNSSRRTGPVIDDARAFVKDVDAVFQDKREKYDEFVKLLQDFGAKRIDRRVVKEGVMELFKDHQDLLPRFNIFLPPGHEISLPLDDDLQQHDRSPLEDEAMVFLTAVKVTFEDKMEKFNEFLKLLADYRAKRIDVRVVKEGMMELFKDHKNLLPGFNTFLPEGHKISIPLDDDLQQRDRSALEDEATAFLTAVKVTFKDKMEMFEEFLKLLADYHAKRLDVRVMKEGMMELFKDHKNLLLGFNTFLPEGHKISLPLDGDEQQDEDGSELKDAQKGDEQ*