AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005249

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005249 MtrunA17_Chr1g0205831 58.480 171 34 2 1 171 46 179 2.79e-56 175
MsaG005249 MtrunA17_Chr1g0205831 53.030 66 31 0 53 118 6 71 7.22e-16 71.6
MsaG005249 MtrunA17_Chr1g0205821 62.295 122 40 1 2 123 58 173 5.60e-47 151
MsaG005249 MtrunA17_Chr1g0205821 53.012 83 39 0 46 128 10 92 9.93e-22 87.0
MsaG005249 MtrunA17_Chr1g0205811 51.887 106 50 1 52 157 17 121 3.43e-29 104
MsaG005249 MtrunA17_Chr1g0205811 55.814 43 19 0 1 43 58 100 6.63e-11 57.0
MsaG005249 MtrunA17_Chr4g0075971 44.628 121 58 1 1 121 133 244 3.29e-25 101
MsaG005249 MtrunA17_Chr4g0075971 44.853 136 60 4 2 136 54 175 9.32e-22 91.7
MsaG005249 MtrunA17_Chr4g0075861 42.975 121 60 1 1 121 133 244 9.95e-25 99.0
MsaG005249 MtrunA17_Chr4g0075861 43.704 135 63 4 2 136 54 175 1.22e-20 87.4
MsaG005249 MtrunA17_Chr1g0205801 55.172 87 38 1 52 138 15 100 2.82e-24 91.7
MsaG005249 MtrunA17_Chr1g0206771 35.652 115 61 1 3 117 62 163 9.82e-20 82.0
MsaG005249 MtrunA17_Chr3g0077891 38.095 126 69 3 2 123 54 174 7.47e-18 76.6
MsaG005249 MtrunA17_Chr3g0077911 43.220 118 59 3 2 118 50 160 2.11e-17 75.1
MsaG005249 MtrunA17_Chr3g0077911 38.182 110 64 2 55 164 14 119 1.64e-15 70.1
MsaG005249 MtrunA17_Chr1g0206781 42.105 95 50 1 35 129 2 91 3.60e-17 73.6
MsaG005249 MtrunA17_Chr7g0218891 37.097 124 68 3 3 124 147 262 3.26e-16 74.3
MsaG005249 MtrunA17_Chr7g0218891 34.307 137 78 3 3 137 61 187 3.76e-15 71.2
MsaG005249 MtrunA17_Chr3g0077861 41.525 118 61 3 2 118 50 160 1.05e-15 70.9
MsaG005249 MtrunA17_Chr3g0077861 42.222 90 47 3 47 136 7 91 1.35e-11 60.1
MsaG005249 MtrunA17_Chr8g0364231 51.351 74 35 1 52 125 45 117 2.30e-15 73.2
MsaG005249 MtrunA17_Chr8g0364231 37.273 110 63 2 2 111 87 190 1.63e-11 62.0
MsaG005249 MtrunA17_Chr3g0077881 39.200 125 62 4 2 120 54 170 5.35e-15 69.3
MsaG005249 MtrunA17_Chr3g0077881 38.136 118 68 2 52 164 11 128 4.03e-11 58.9
MsaG005249 MtrunA17_Chr3g0077941 39.370 127 65 3 2 123 54 173 1.18e-14 68.2
MsaG005249 MtrunA17_Chr5g0415031 36.697 109 64 1 52 155 46 154 3.65e-14 69.7
MsaG005249 MtrunA17_Chr5g0415031 32.727 110 68 1 2 111 88 191 2.94e-11 61.6
MsaG005249 MtrunA17_Chr6g0452001 37.647 85 53 0 52 136 66 150 9.37e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005249 AT1G27260.1 42.609 115 64 2 1 115 47 159 3.54e-19 81.6
MsaG005249 AT1G27260.1 48.000 75 37 1 50 122 2 76 1.90e-12 63.5
MsaG005249 AT1G24250.1 31.977 172 101 4 3 160 61 230 3.09e-15 71.6
MsaG005249 AT1G23810.1 31.694 183 115 5 3 182 61 236 6.52e-15 70.5
MsaG005249 AT1G24200.1 44.578 83 43 2 35 117 3 82 1.10e-12 63.9
MsaG005249 AT1G24230.1 28.495 186 104 4 2 182 60 221 1.15e-12 64.7
MsaG005249 AT1G24190.3 43.956 91 43 3 35 122 3 88 4.10e-12 64.3
MsaG005249 AT1G24190.1 43.956 91 43 3 35 122 3 88 4.22e-12 64.3
MsaG005249 AT5G15040.1 37.681 69 43 0 50 118 4 72 4.36e-12 59.7
MsaG005249 AT5G15040.3 37.681 69 43 0 50 118 4 72 4.36e-12 59.7
MsaG005249 AT5G15040.2 37.681 69 43 0 50 118 4 72 4.36e-12 59.7
MsaG005249 AT1G24190.2 43.956 91 43 3 35 122 3 88 4.51e-12 64.3
MsaG005249 AT1G70060.2 43.836 73 41 0 52 124 17 89 1.39e-11 62.8
MsaG005249 AT1G70060.1 43.836 73 41 0 52 124 17 89 1.39e-11 62.8

Find 22 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCCAAAAGTAAATCTTTA+TGG 0.189829 1:-79429664 None:intergenic
GTCCCAAAAGTAAATCTTTA+TGG 0.189829 1:-79429940 None:intergenic
AGACCATAAAGATTTACTTT+TGG 0.338463 1:+79429661 MsaT005249.1:CDS
AGACCATAAAGATTTACTTT+TGG 0.338463 1:+79429937 MsaT005249.1:CDS
AGTTGCATTTCAAGATAAGA+TGG 0.422713 1:+79429820 MsaT005249.1:CDS
ACACAAAATCTCACTTCCAT+TGG 0.512949 1:+79429985 MsaT005249.1:CDS
ACACAAAATCTCACTTCCAT+TGG 0.514630 1:+79429709 MsaT005249.1:CDS
GATGAACAACAAGGTGATGA+TGG 0.529706 1:+79430013 MsaT005249.1:CDS
CTCAACACTTGGTTGCCAGC+GGG 0.549872 1:+79429686 MsaT005249.1:CDS
TGAACTTCAAGTTGATGAAG+TGG 0.554268 1:+79430099 MsaT005249.1:CDS
CTCAACACTTGGTTGCCAGC+AGG 0.555626 1:+79429962 MsaT005249.1:CDS
GATGCTGAACAACAAGGTGA+TGG 0.558029 1:+79430061 MsaT005249.1:CDS
TGTTGTTCATCACCATCCAA+TGG 0.575143 1:-79429725 None:intergenic
TGTTGTTCATCACCATCCAA+TGG 0.575143 1:-79430001 None:intergenic
AAAATCTCACTTCCATTGGA+TGG 0.592611 1:+79429713 MsaT005249.1:CDS
AAAATCTCACTTCCATTGGA+TGG 0.592611 1:+79429989 MsaT005249.1:CDS
GGTGATGAACAACAAGGTGA+TGG 0.600828 1:+79429734 MsaT005249.1:CDS
ACTCAACACTTGGTTGCCAG+CGG 0.601418 1:+79429685 MsaT005249.1:CDS
TGAACAACAAGGTGATGGAT+CGG 0.624867 1:+79429739 MsaT005249.1:CDS
CAGAAAGATGCTGAACAACA+AGG 0.627330 1:+79430055 MsaT005249.1:CDS
TTGGATGGTGATGAACAACA+AGG 0.791947 1:+79429728 MsaT005249.1:CDS
TTGGATGGTGATGAACAACA+AGG 0.791947 1:+79430004 MsaT005249.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 79429647 79430195 79429647 ID=MsaG005249
Chr1 mRNA 79429647 79430195 79429647 ID=MsaT005249.1;Parent=MsaG005249
Chr1 exon 79429647 79430195 79429647 ID=MsaT005249.1.exon1;Parent=MsaT005249.1
Chr1 CDS 79429647 79430195 79429647 ID=cds.MsaT005249.1;Parent=MsaT005249.1
Gene Sequence

>MsaT005249.1

ATGGAGTTGTTTAAAGACCATAAAGATTTACTTTTGGGACTCAACACTTGGTTGCCAGCGGGACACAAAATCTCACTTCCATTGGATGGTGATGAACAACAAGGTGATGGATCGGCGCTCAAAGATGAATCAGCACTCGCAGATGCAACAAAGTCGTTTGCAACAGCAGTTAAAGTTGCATTTCAAGATAAGATGGATAAGTATGATGAATTCTTAAAACTCATAATTGATTGTCGTACAAAAAGAATTGATTTTAGAGTTTTCAAAGAAGGAATGGTGGAGTTGTTTAAAGACCATAAAGATTTACTTTTGGGACTCAACACTTGGTTGCCAGCAGGACACAAAATCTCACTTCCATTGGATGGTGATGAACAACAAGGTGATGATGGATTAGAGTTGAAAGATGCACAGAAAGATGCTGAACAACAAGGTGATGGATTAGAGTACAAAGATGAACTTCAAGTTGATGAAGTGGCACTCAAACTCAAAGATGAGCAGAAAGATGACGAACAACTCATCTTTAAATTGTATCAAGTGCATAGAGATTGA
Protein sequence

>MsaT005249.1

MELFKDHKDLLLGLNTWLPAGHKISLPLDGDEQQGDGSALKDESALADATKSFATAVKVAFQDKMDKYDEFLKLIIDCRTKRIDFRVFKEGMVELFKDHKDLLLGLNTWLPAGHKISLPLDGDEQQGDDGLELKDAQKDAEQQGDGLEYKDELQVDEVALKLKDEQKDDEQLIFKLYQVHRD*