AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005251


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005251 MtrunA17_Chr1g0205861 49.505 101 51 0 3 103 2 102 4.01e-28 100
MsaG005251 MtrunA17_Chr7g0256011 52.809 89 41 1 1 89 1 88 6.98e-22 85.1
MsaG005251 MtrunA17_Chr1g0206771 48.611 72 37 0 11 82 10 81 3.74e-16 72.0
MsaG005251 MtrunA17_Chr1g0206771 46.269 67 36 0 15 81 93 159 9.50e-13 62.8
MsaG005251 MtrunA17_Chr3g0077891 44.578 83 45 1 11 92 93 175 1.52e-15 70.1
MsaG005251 MtrunA17_Chr5g0415031 41.558 77 45 0 9 85 35 111 1.67e-14 70.1
MsaG005251 MtrunA17_Chr3g0077941 45.122 82 44 1 12 92 93 174 3.56e-14 66.6
MsaG005251 MtrunA17_Chr8g0364231 42.308 78 44 1 8 85 34 110 1.11e-13 67.8
MsaG005251 MtrunA17_Chr1g0206781 38.095 84 52 0 8 91 2 85 2.24e-13 63.2
MsaG005251 MtrunA17_Chr1g0205811 43.210 81 43 1 17 94 14 94 7.78e-13 61.6
MsaG005251 MtrunA17_Chr4g0075971 40.187 107 60 2 16 121 88 191 9.39e-13 65.1
MsaG005251 MtrunA17_Chr4g0075971 32.743 113 72 2 20 128 175 287 2.70e-11 60.8
MsaG005251 MtrunA17_Chr3g0077881 42.169 83 47 1 11 92 93 175 1.46e-12 62.4
MsaG005251 MtrunA17_Chr4g0075861 38.318 107 62 2 16 121 88 191 3.47e-12 63.2
MsaG005251 MtrunA17_Chr4g0075861 33.628 113 71 2 20 128 175 287 8.12e-12 62.0
MsaG005251 MtrunA17_Chr1g0205801 46.875 64 34 0 15 78 10 73 5.05e-12 59.3
MsaG005251 MtrunA17_Chr6g0452001 41.176 68 40 0 13 80 59 126 1.82e-11 61.2
MsaG005251 MtrunA17_Chr1g0205821 45.902 61 33 0 18 78 100 160 2.09e-11 59.3
MsaG005251 MtrunA17_Chr1g0205821 46.774 62 33 0 17 78 13 74 3.43e-11 58.5
MsaG005251 MtrunA17_Chr1g0205831 48.276 58 30 0 21 78 6 63 7.59e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005251 AT3G01320.2 36.782 87 55 0 13 99 53 139 7.84e-13 65.9
MsaG005251 AT3G01320.1 36.782 87 55 0 13 99 53 139 8.00e-13 65.5
MsaG005251 AT1G70060.2 42.105 76 43 1 5 80 3 77 2.48e-12 64.3
MsaG005251 AT1G70060.1 42.105 76 43 1 5 80 3 77 2.48e-12 64.3
MsaG005251 AT1G24190.3 39.474 76 45 1 5 80 3 77 7.33e-12 62.8
MsaG005251 AT1G24190.1 39.474 76 45 1 5 80 3 77 7.99e-12 62.8
MsaG005251 AT1G24190.2 39.474 76 45 1 5 80 3 77 8.22e-12 62.8
MsaG005251 AT5G15040.1 42.188 64 37 0 15 78 1 64 1.86e-11 57.4
MsaG005251 AT5G15040.3 42.188 64 37 0 15 78 1 64 1.86e-11 57.4
MsaG005251 AT5G15040.2 42.188 64 37 0 15 78 1 64 1.86e-11 57.4

Find 15 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCAAGCATTTGATTAAAT+TGG 0.233339 1:+79442099 None:intergenic
TAGAGATCTCTTTAGCATTA+AGG 0.235668 1:+79442199 None:intergenic
TGACCCTATTTGATCGAGTT+TGG 0.318141 1:+79442046 None:intergenic
ATTGCCAAACTCGATCAAAT+AGG 0.324623 1:-79442050 MsaT005251.1:CDS
AGTTGTGTTTCAAGATAAGA+TGG 0.435039 1:-79442348 MsaT005251.1:CDS
ATGCAAAACAGCACATCAAA+CGG 0.448989 1:-79442148 MsaT005251.1:CDS
TTGCCAAACTCGATCAAATA+GGG 0.483923 1:-79442049 MsaT005251.1:CDS
TGCAAAACAGCACATCAAAC+GGG 0.503847 1:-79442147 MsaT005251.1:CDS
CAAACGGGATCGATTCAAGT+TGG 0.545140 1:-79442132 MsaT005251.1:CDS
AAGGATTACAGTGGTGATGC+TGG 0.561781 1:-79442422 MsaT005251.1:CDS
ACGGGATCGATTCAAGTTGG+AGG 0.593315 1:-79442129 MsaT005251.1:CDS
AAAATGGAGGAGTTGTTCGA+TGG 0.597043 1:-79442251 MsaT005251.1:CDS
TCAGAAACTACTAAGCATGA+AGG 0.638522 1:-79442399 MsaT005251.1:CDS
GCAGAGAACATGAAGAAGCG+AGG 0.677188 1:-79441987 MsaT005251.1:CDS
CGACTGATGAAGGATTACAG+TGG 0.795711 1:-79442431 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 79441962 79442447 79441962 ID=MsaG005251
Chr1 mRNA 79441962 79442447 79441962 ID=MsaT005251.1;Parent=MsaG005251
Chr1 exon 79441962 79442447 79441962 ID=MsaT005251.1.exon1;Parent=MsaT005251.1
Chr1 CDS 79441962 79442447 79441962 ID=cds.MsaT005251.1;Parent=MsaT005251.1
Gene Sequence

>MsaT005251.1

ATGAAGGATTACAGTGGTGATGCTGGTCAGAAACTACTAAGCATGAAGGAAGCATCAGCGTTTGTAAATGATGTAAAAGTTGTGTTTCAAGATAAGATGGAAAAGTATGATGAGTTTGTGAAAGTTTTGTATGATTTCAAGGCTATGAGAATTGATCAAGAAAGTGCCACAGCAAAAATGGAGGAGTTGTTCGATGGACATTCAGATTTGATTATGAAATTAAACTCCTTAATGCTAAAGAGATCTCTAATGACTAACAATGATCATAATGACACTGATGCAAAACAGCACATCAAACGGGATCGATTCAAGTTGGAGGTAATTGACCAATTTAATCAAATGCTTGACAAACTAGAGAAATTTGAGTTGAAAGTGATTGCCAAACTCGATCAAATAGGGTCAAGTGCTCAACATTCGACTAAAGCAATATTAGACATTGCAGAGAACATGAAGAAGCGAGGCGTGACAAACGTGAGTCAAAAGTGA

Protein sequence

>MsaT005251.1

MKDYSGDAGQKLLSMKEASAFVNDVKVVFQDKMEKYDEFVKVLYDFKAMRIDQESATAKMEELFDGHSDLIMKLNSLMLKRSLMTNNDHNDTDAKQHIKRDRFKLEVIDQFNQMLDKLEKFELKVIAKLDQIGSSAQHSTKAILDIAENMKKRGVTNVSQK*