AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005252


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005252 MtrunA17_Chr1g0205861 72.807 114 25 2 8 121 12 119 8.11e-51 157
MsaG005252 MtrunA17_Chr1g0206781 45.455 88 43 1 8 95 7 89 2.88e-22 85.1
MsaG005252 MtrunA17_Chr1g0206771 54.930 71 32 0 9 79 13 83 4.61e-20 80.9
MsaG005252 MtrunA17_Chr1g0206771 54.286 70 32 0 11 80 94 163 3.55e-18 76.3
MsaG005252 MtrunA17_Chr1g0205811 47.368 76 40 0 5 80 7 82 8.32e-20 79.0
MsaG005252 MtrunA17_Chr8g0364231 50.000 76 38 0 5 80 35 110 1.35e-19 83.6
MsaG005252 MtrunA17_Chr1g0205821 46.250 80 43 0 1 80 88 167 6.89e-19 77.8
MsaG005252 MtrunA17_Chr1g0205821 48.649 74 38 0 7 80 8 81 3.25e-14 65.9
MsaG005252 MtrunA17_Chr5g0415031 49.315 73 37 0 8 80 39 111 8.51e-19 81.3
MsaG005252 MtrunA17_Chr1g0205801 53.623 69 32 0 12 80 12 80 2.29e-18 74.7
MsaG005252 MtrunA17_Chr6g0452001 42.222 90 45 1 8 97 59 141 2.97e-18 79.7
MsaG005252 MtrunA17_Chr7g0256011 49.275 69 35 0 12 80 16 84 3.22e-18 74.7
MsaG005252 MtrunA17_Chr3g0077891 45.455 88 43 2 2 84 89 176 1.54e-17 74.3
MsaG005252 MtrunA17_Chr3g0077891 42.029 69 39 1 12 79 8 76 2.91e-11 57.8
MsaG005252 MtrunA17_Chr3g0077861 51.515 66 31 1 11 75 89 154 1.77e-15 68.9
MsaG005252 MtrunA17_Chr3g0077941 44.186 86 43 2 4 84 90 175 3.13e-15 68.2
MsaG005252 MtrunA17_Chr3g0077941 42.029 69 39 1 12 79 8 76 4.87e-11 57.4
MsaG005252 MtrunA17_Chr6g0452021 41.026 78 46 0 8 85 39 116 4.35e-15 70.5
MsaG005252 MtrunA17_Chr8g0364021 43.210 81 44 1 1 79 31 111 1.46e-14 67.8
MsaG005252 MtrunA17_Chr3g0077911 51.515 66 31 1 11 75 89 154 1.51e-14 66.2
MsaG005252 MtrunA17_Chr3g0077911 40.845 71 39 1 9 79 5 72 4.34e-11 57.0
MsaG005252 MtrunA17_Chr7g0218911 46.774 62 33 0 19 80 5 66 2.37e-14 64.7
MsaG005252 MtrunA17_Chr4g0075971 46.970 66 35 0 15 80 175 240 5.03e-14 67.4
MsaG005252 MtrunA17_Chr4g0075971 46.575 73 39 0 8 80 85 157 4.35e-13 64.7
MsaG005252 MtrunA17_Chr4g0075971 42.857 84 41 2 11 90 11 91 7.71e-12 61.2
MsaG005252 MtrunA17_Chr1g0205831 47.059 68 36 0 12 79 2 69 8.79e-14 64.7
MsaG005252 MtrunA17_Chr1g0205831 48.485 66 34 0 15 80 76 141 1.88e-13 63.5
MsaG005252 MtrunA17_Chr4g0075861 47.945 73 38 0 8 80 85 157 2.04e-13 65.5
MsaG005252 MtrunA17_Chr4g0075861 46.970 66 35 0 15 80 175 240 2.34e-13 65.5
MsaG005252 MtrunA17_Chr4g0075861 53.846 52 24 0 28 79 25 76 6.34e-11 58.5
MsaG005252 MtrunA17_Chr3g0077881 42.500 80 45 1 2 80 89 168 1.02e-12 61.6
MsaG005252 MtrunA17_Chr3g0077881 38.554 83 50 1 12 93 8 90 6.00e-12 59.7
MsaG005252 MtrunA17_Chr1g0209201 47.541 61 31 1 19 79 23 82 2.72e-12 60.1
MsaG005252 MtrunA17_Chr7g0218891 44.928 69 36 1 13 79 186 254 3.79e-12 61.6
MsaG005252 MtrunA17_Chr7g0218891 46.875 64 32 1 13 74 14 77 9.17e-12 60.5
MsaG005252 MtrunA17_Chr7g0218891 45.312 64 33 1 13 74 100 163 9.54e-12 60.5
MsaG005252 MtrunA17_Chr2g0328701 44.048 84 40 2 11 90 28 108 3.95e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005252 AT1G24190.3 51.250 80 35 1 5 80 3 82 3.96e-21 88.2
MsaG005252 AT1G24190.1 51.250 80 35 1 5 80 3 82 4.20e-21 88.2
MsaG005252 AT1G24190.2 51.250 80 35 1 5 80 3 82 4.28e-21 88.2
MsaG005252 AT1G70060.2 52.055 73 35 0 8 80 10 82 1.42e-19 84.0
MsaG005252 AT1G70060.1 52.055 73 35 0 8 80 10 82 1.42e-19 84.0
MsaG005252 AT3G01320.1 47.945 73 38 0 8 80 53 125 4.32e-19 82.4
MsaG005252 AT3G01320.2 47.945 73 38 0 8 80 53 125 4.54e-19 82.4
MsaG005252 AT1G24200.1 36.765 136 72 5 1 130 1 128 2.46e-18 77.4
MsaG005252 AT5G15020.1 45.333 75 41 0 8 82 48 122 5.27e-18 79.3
MsaG005252 AT5G15020.2 45.333 75 41 0 8 82 48 122 5.42e-18 79.3
MsaG005252 AT1G59890.2 44.928 69 38 0 12 80 45 113 2.57e-15 71.6
MsaG005252 AT1G59890.4 44.928 69 38 0 12 80 45 113 2.67e-15 71.6
MsaG005252 AT4G12020.10 46.667 75 39 1 6 80 302 375 2.69e-15 71.6
MsaG005252 AT1G59890.1 44.928 69 38 0 12 80 45 113 2.75e-15 71.6
MsaG005252 AT4G12020.4 46.667 75 39 1 6 80 302 375 2.80e-15 71.6
MsaG005252 AT4G12020.3 46.667 75 39 1 6 80 302 375 2.82e-15 71.6
MsaG005252 AT1G59890.3 44.928 69 38 0 12 80 45 113 2.83e-15 71.6
MsaG005252 AT4G12020.6 46.667 75 39 1 6 80 302 375 2.84e-15 71.6
MsaG005252 AT4G12020.8 46.667 75 39 1 6 80 302 375 2.84e-15 71.6
MsaG005252 AT4G12020.11 46.667 75 39 1 6 80 302 375 2.90e-15 71.6
MsaG005252 AT4G12020.2 46.667 75 39 1 6 80 302 375 2.91e-15 71.6
MsaG005252 AT4G12020.5 46.667 75 39 1 6 80 235 308 2.93e-15 71.6
MsaG005252 AT4G12020.1 46.667 75 39 1 6 80 302 375 3.01e-15 71.6
MsaG005252 AT4G12020.9 46.667 75 39 1 6 80 302 375 3.01e-15 71.6
MsaG005252 AT4G12020.7 46.667 75 39 1 6 80 302 375 3.04e-15 71.6
MsaG005252 AT1G27250.1 39.024 82 50 0 8 89 17 98 5.83e-15 67.0
MsaG005252 AT5G15025.1 38.158 76 47 0 7 82 42 117 9.06e-15 66.2
MsaG005252 AT1G70030.3 41.558 77 43 1 8 82 18 94 3.19e-14 64.7
MsaG005252 AT1G70030.1 32.812 128 73 4 8 130 7 126 3.55e-14 65.5
MsaG005252 AT1G70030.2 41.558 77 43 1 8 82 18 94 6.47e-14 64.3
MsaG005252 AT1G27280.1 38.571 70 43 0 9 78 78 147 1.47e-12 62.4
MsaG005252 AT1G24230.1 43.478 69 38 1 7 74 9 77 1.49e-12 62.8
MsaG005252 AT1G10450.2 40.580 69 41 0 12 80 33 101 2.55e-12 63.2
MsaG005252 AT1G10450.1 40.580 69 41 0 12 80 33 101 2.55e-12 63.2
MsaG005252 AT1G10450.3 40.580 69 41 0 12 80 84 152 2.69e-12 63.2
MsaG005252 AT1G27220.1 36.486 74 47 0 8 81 10 83 3.37e-12 60.8
MsaG005252 AT1G23810.1 42.188 64 37 0 9 72 106 169 5.81e-12 61.2
MsaG005252 AT1G24210.1 40.000 75 44 1 7 81 9 82 4.55e-11 57.4
MsaG005252 AT1G24250.1 42.188 64 37 0 9 72 117 180 7.77e-11 58.2

Find 27 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATAAAATATTAGAGATTT+TGG 0.199052 1:-79445883 MsaT005252.1:CDS
TGAAAGTCCTAAGCGATTAC+AGG 0.315902 1:-79446133 MsaT005252.1:CDS
AGGATGGAGGAGCTGTTTAA+AGG 0.330709 1:-79446077 MsaT005252.1:CDS
CTCTTGGCAATGCAGGATAA+AGG 0.369224 1:-79446245 None:intergenic
AAGAACCCACCTGATCGAAT+AGG 0.409769 1:-79445957 MsaT005252.1:CDS
AAACGCTATTGCTTCTTCCT+TGG 0.423218 1:+79446211 None:intergenic
CAGTGATGACACTGGAGAAA+AGG 0.440953 1:-79446000 MsaT005252.1:CDS
TCACTGATTTGAGATGGCTT+TGG 0.444928 1:+79446017 None:intergenic
GGATGGAGGAGCTGTTTAAA+GGG 0.456905 1:-79446076 MsaT005252.1:CDS
AGAACCCACCTGATCGAATA+GGG 0.457483 1:-79445956 MsaT005252.1:CDS
ATTTGCGACCCTATTCGATC+AGG 0.474283 1:+79445948 None:intergenic
TTGAGATGGCTTTGGCGTAA+AGG 0.479351 1:+79446025 None:intergenic
AGTTAAACTTCTGTCTCAAA+AGG 0.482056 1:-79445907 MsaT005252.1:CDS
TGCGACCCTATTCGATCAGG+TGG 0.509774 1:+79445951 None:intergenic
GCTAAAAGAATTGATATAGC+AGG 0.520011 1:-79446110 MsaT005252.1:CDS
GTGTCATCACTGATTTGAGA+TGG 0.558119 1:+79446011 None:intergenic
GAAAGTCCTAAGCGATTACA+GGG 0.564984 1:-79446132 MsaT005252.1:CDS
AGTTAAGTTTCAAGACAAGA+GGG 0.571152 1:-79446174 MsaT005252.1:CDS
AAGTTAAGTTTCAAGACAAG+AGG 0.577150 1:-79446175 MsaT005252.1:CDS
AGCAGGTGTGACAACAAGGA+TGG 0.585519 1:-79446093 MsaT005252.1:CDS
GCGACCCTATTCGATCAGGT+GGG 0.613931 1:+79445952 None:intergenic
ATATAGCAGGTGTGACAACA+AGG 0.623415 1:-79446097 MsaT005252.1:CDS
TCTCAAATCAGTGATGACAC+TGG 0.643527 1:-79446008 MsaT005252.1:CDS
AGGTGTGACAACAAGGATGG+AGG 0.648111 1:-79446090 MsaT005252.1:CDS
TAAAGGTGGTGGTGTAACCA+AGG 0.651615 1:-79446228 MsaT005252.1:CDS
GCAATGCAGGATAAAGGTGG+TGG 0.669430 1:-79446239 MsaT005252.1:CDS
TTGGCAATGCAGGATAAAGG+TGG 0.685630 1:-79446242 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 79445863 79446258 79445863 ID=MsaG005252
Chr1 mRNA 79445863 79446258 79445863 ID=MsaT005252.1;Parent=MsaG005252
Chr1 exon 79445863 79446258 79445863 ID=MsaT005252.1.exon1;Parent=MsaT005252.1
Chr1 CDS 79445863 79446258 79445863 ID=cds.MsaT005252.1;Parent=MsaT005252.1
Gene Sequence

>MsaT005252.1

ATGCAGGATAAAGGTGGTGGTGTAACCAAGGAAGAAGCAATAGCGTTTGTATGTGATGTAAAAGTTAAGTTTCAAGACAAGAGGGAAAAGTATGATGAATTTTTGAAAGTCCTAAGCGATTACAGGGCTAAAAGAATTGATATAGCAGGTGTGACAACAAGGATGGAGGAGCTGTTTAAAGGGCATTCAGATTTGATTTTGAAATTCAAATCCTTTACGCCAAAGCCATCTCAAATCAGTGATGACACTGGAGAAAAGGAAGAAGACACTGATGAACAGAAGAACCCACCTGATCGAATAGGGTCGCAAATTCAACTTATATCTCAAGAAGTTAAACTTCTGTCTCAAAAGGCTGATAAAATATTAGAGATTTTGGAGGAGAAGAAGAAAGTATAA

Protein sequence

>MsaT005252.1

MQDKGGGVTKEEAIAFVCDVKVKFQDKREKYDEFLKVLSDYRAKRIDIAGVTTRMEELFKGHSDLILKFKSFTPKPSQISDDTGEKEEDTDEQKNPPDRIGSQIQLISQEVKLLSQKADKILEILEEKKKV*