AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005265

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005265 MtrunA17_Chr2g0322731 51.641 701 299 10 40 709 74 765 0.0 684
MsaG005265 MtrunA17_Chr4g0024351 50.789 697 305 10 43 709 41 729 0.0 666
MsaG005265 MtrunA17_Chr5g0394111 44.017 702 298 14 42 710 8 647 9.20e-178 522
MsaG005265 MtrunA17_Chr8g0350321 41.963 703 354 12 59 709 106 806 2.97e-176 524
MsaG005265 MtrunA17_Chr7g0246631 41.667 696 341 15 67 709 91 774 5.15e-175 520
MsaG005265 MtrunA17_Chr1g0203671 39.515 701 364 13 65 709 93 789 3.76e-165 494
MsaG005265 MtrunA17_Chr6g0472471 36.312 705 367 15 73 709 75 765 7.11e-140 429
MsaG005265 MtrunA17_Chr6g0481041 36.040 702 386 15 66 709 27 723 7.42e-137 419
MsaG005265 MtrunA17_Chr2g0331581 35.407 675 368 13 73 709 145 789 1.14e-136 421
MsaG005265 MtrunA17_Chr5g0410711 35.327 719 387 18 59 713 78 782 8.53e-133 411
MsaG005265 MtrunA17_Chr1g0205981 85.106 188 26 1 122 307 1 188 3.53e-104 316
MsaG005265 MtrunA17_Chr7g0224451 36.806 432 215 12 65 482 14 401 7.36e-78 256
MsaG005265 MtrunA17_Chr2g0292731 32.283 508 300 14 209 677 12 514 5.44e-71 241
MsaG005265 MtrunA17_Chr2g0292741 32.359 479 276 11 209 646 4 475 4.86e-70 238
MsaG005265 MtrunA17_Chr2g0292761 29.483 580 349 17 149 710 4 541 4.01e-59 209
MsaG005265 MtrunA17_Chr7g0224481 35.501 369 181 11 65 419 14 339 7.15e-56 194
MsaG005265 MtrunA17_Chr2g0319671 45.930 172 87 2 301 472 103 268 9.20e-45 162
MsaG005265 MtrunA17_Chr7g0224491 33.968 315 159 11 422 709 1 293 4.28e-42 155
MsaG005265 MtrunA17_Chr2g0319691 53.153 111 50 1 362 472 1 109 7.44e-33 124
MsaG005265 MtrunA17_Chr5g0419821 41.667 132 75 1 58 187 23 154 1.48e-25 103
MsaG005265 MtrunA17_Chr2g0319681 32.301 226 141 5 491 709 5 225 1.44e-23 100
MsaG005265 MtrunA17_Chr2g0319701 32.843 204 124 6 511 709 28 223 1.87e-20 91.3
MsaG005265 MtrunA17_Chr2g0292391 21.353 754 416 27 53 712 2 672 4.67e-18 89.0
MsaG005265 MtrunA17_Chr2g0326731 22.892 664 354 26 58 647 10 589 8.48e-16 81.6
MsaG005265 MtrunA17_Chr2g0304191 33.588 131 82 2 261 386 1 131 4.56e-15 72.8
MsaG005265 MtrunA17_Chr7g0224461 31.452 124 61 4 600 709 1 114 7.54e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005265 AT4G21750.2 49.281 765 318 14 9 710 1 758 0.0 702
MsaG005265 AT4G21750.1 49.281 765 318 14 9 710 1 758 0.0 702
MsaG005265 AT4G21750.3 49.281 765 318 14 9 710 1 758 0.0 702
MsaG005265 AT4G21750.4 49.281 765 318 14 9 710 1 758 0.0 702
MsaG005265 AT4G04890.2 52.770 686 284 13 60 709 54 735 0.0 697
MsaG005265 AT4G04890.1 52.770 686 284 13 60 709 54 735 0.0 697
MsaG005265 AT1G05230.8 49.282 696 303 10 44 709 41 716 0.0 655
MsaG005265 AT1G05230.4 49.282 696 303 10 44 709 41 716 0.0 655
MsaG005265 AT1G05230.9 49.282 696 303 10 44 709 41 716 0.0 655
MsaG005265 AT1G05230.10 49.282 696 303 10 44 709 41 716 0.0 655
MsaG005265 AT1G05230.7 49.282 696 303 10 44 709 41 716 0.0 655
MsaG005265 AT1G05230.11 49.282 696 303 10 44 709 41 716 0.0 655
MsaG005265 AT1G05230.2 49.282 696 303 10 44 709 41 716 0.0 655
MsaG005265 AT1G05230.6 49.282 696 303 10 44 709 41 716 0.0 655
MsaG005265 AT1G05230.5 49.282 696 303 10 44 709 41 716 0.0 655
MsaG005265 AT1G05230.1 49.282 696 303 10 44 709 41 716 0.0 655
MsaG005265 AT1G05230.3 49.138 696 302 11 44 709 41 714 0.0 648
MsaG005265 AT1G05230.12 49.654 578 246 8 44 591 41 603 0.0 553
MsaG005265 AT3G61150.1 41.384 708 338 16 66 709 109 803 7.88e-175 520
MsaG005265 AT4G00730.1 41.369 701 334 14 59 709 125 798 4.06e-170 508
MsaG005265 AT2G32370.1 40.086 696 355 15 46 706 49 717 6.81e-158 474
MsaG005265 AT5G52170.2 37.482 691 338 14 58 709 48 683 4.33e-143 435
MsaG005265 AT5G52170.3 37.609 686 339 14 58 709 48 678 1.00e-142 434
MsaG005265 AT5G52170.1 37.609 686 339 14 58 709 48 678 1.00e-142 434
MsaG005265 AT1G73360.1 36.152 686 393 12 66 709 31 713 6.50e-133 409
MsaG005265 AT1G79840.3 35.432 683 362 16 66 709 42 684 2.01e-131 404
MsaG005265 AT1G79840.6 35.432 683 362 16 66 709 42 684 2.01e-131 404
MsaG005265 AT1G79840.1 35.432 683 362 16 66 709 100 742 5.78e-131 405
MsaG005265 AT1G79840.2 35.432 683 362 16 66 709 129 771 3.77e-130 404
MsaG005265 AT1G17920.2 36.018 683 363 15 73 709 27 681 1.14e-124 387
MsaG005265 AT1G17920.1 36.018 683 363 15 73 709 27 681 1.14e-124 387
MsaG005265 AT5G46880.3 34.199 693 376 19 59 685 104 782 6.87e-113 360
MsaG005265 AT5G46880.1 34.199 693 376 19 59 685 104 782 6.87e-113 360
MsaG005265 AT5G46880.2 34.199 693 376 19 59 685 104 782 6.87e-113 360
MsaG005265 AT3G03260.1 34.632 693 380 17 68 709 24 694 1.93e-112 355
MsaG005265 AT4G00730.2 41.126 462 218 8 59 491 125 561 5.05e-107 337
MsaG005265 AT1G79840.4 35.125 558 289 14 66 587 100 620 3.69e-105 334
MsaG005265 AT4G17710.1 33.185 675 379 16 59 711 81 705 1.32e-103 332
MsaG005265 AT4G25530.1 31.313 693 399 13 54 709 30 682 2.42e-98 318
MsaG005265 AT5G17320.1 31.285 716 409 23 59 709 18 715 2.38e-87 290
MsaG005265 AT1G34650.1 31.511 695 405 20 74 709 23 705 4.16e-85 283
MsaG005265 AT3G03260.2 35.161 529 292 12 68 559 24 538 7.24e-85 278
MsaG005265 AT1G79840.5 36.222 450 227 13 66 499 100 505 1.51e-81 269
MsaG005265 AT5G07260.1 26.157 562 311 21 126 652 3 495 2.78e-35 141
MsaG005265 AT5G07260.2 25.719 556 316 19 126 652 3 490 6.49e-34 137
MsaG005265 AT2G34710.1 22.131 732 403 27 73 710 28 686 1.62e-21 100
MsaG005265 AT5G60690.1 22.193 748 389 31 63 709 17 672 5.80e-17 85.9

Find 163 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTTCTTCAATCGGTTATT+TGG 0.114581 1:-79607072 MsaT005265.1:CDS
CTCTTGCTGGATCCCCTTTA+TGG 0.142742 1:-79605601 MsaT005265.1:CDS
GAGCTTCATTTCTAGAATTT+TGG 0.158862 1:+79607033 None:intergenic
CCCCGTGAGTTTCGGACTTT+TGG 0.171515 1:+79605523 None:intergenic
CATTATCCTTTACCTTAATT+TGG 0.181539 1:+79606701 None:intergenic
AATTGAGAATGCTAGATTAA+AGG 0.219959 1:-79606041 MsaT005265.1:CDS
GACATTTCACCAATAGAAAT+TGG 0.229853 1:+79606085 None:intergenic
GAAGCACCACCATATTATTT+TGG 0.238800 1:+79602247 None:intergenic
CGTCCCTCAAAAGATCATTT+TGG 0.241359 1:+79606185 None:intergenic
GGAAGCAAAGCAACAAAATT+TGG 0.246586 1:+79602135 None:intergenic
GAGGATGATCAACCACATTT+TGG 0.247548 1:+79607138 None:intergenic
TTAGTGTTGGAACTTCTATT+TGG 0.254156 1:-79602843 MsaT005265.1:CDS
GAAATTGGATCAGTACAATT+TGG 0.256467 1:+79606100 None:intergenic
GTTGATGGTGGTTAGGATCT+TGG 0.260432 1:+79606994 None:intergenic
GTCCTTCTGTAGCTAGTTGC+TGG 0.266212 1:-79602951 MsaT005265.1:CDS
GTTTCCTTTGCTTGCCATTA+GGG 0.283292 1:+79606788 None:intergenic
ATAACTTCTGGTCTTTCCTT+TGG 0.283937 1:-79603935 MsaT005265.1:CDS
TGTTTCCTTTGCTTGCCATT+AGG 0.288076 1:+79606787 None:intergenic
TAATCTCATTGATATCCTTA+TGG 0.293429 1:-79605450 MsaT005265.1:CDS
AGAAGCACCAAGAAGGGATA+TGG 0.307095 1:-79604331 MsaT005265.1:CDS
CTTCTTTGTTGATGGTGGTT+AGG 0.308209 1:+79606987 None:intergenic
TGTACTGATCCAATTTCTAT+TGG 0.313025 1:-79606094 MsaT005265.1:CDS
CCACTGCCCATATCCCTTCT+TGG 0.314521 1:+79604324 None:intergenic
GTTGCTTTGTCAAAATCATT+AGG 0.315448 1:+79605671 None:intergenic
TCTAAGTTGATTGTCGTTAG+AGG 0.316867 1:+79606063 None:intergenic
AGAACTTGCTGTGGTTTCTA+TGG 0.322944 1:-79605642 MsaT005265.1:CDS
TATAAATCTTTAATAACTTC+TGG 0.327409 1:-79603947 MsaT005265.1:CDS
ATTTATGCTCCGGTTGATAT+TGG 0.334121 1:-79602189 MsaT005265.1:CDS
ATCTACACCTGCATTTGAAA+TGG 0.335392 1:-79605774 MsaT005265.1:CDS
AAACAAGCGCAACCAAATTA+AGG 0.338062 1:-79606713 MsaT005265.1:intron
AATGAGATTAATATGATTCA+TGG 0.340541 1:+79605463 None:intergenic
TGGAACTTCTATTTGGCTTC+CGG 0.345537 1:-79602836 MsaT005265.1:CDS
GTTATTAAAGATTTATAGAA+TGG 0.351722 1:+79603953 None:intergenic
TCTACTCTTCTTGTTGGACT+TGG 0.352812 1:+79601997 None:intergenic
CTCACAATGTTAACTCTTGC+TGG 0.354778 1:-79605614 MsaT005265.1:CDS
GAATTTAACCCCGTGAGTTT+CGG 0.355247 1:+79605515 None:intergenic
CATCTGATTGTGACGATGTA+AGG 0.356882 1:-79602918 MsaT005265.1:CDS
TGCTGAAAATGAGATCTATA+AGG 0.357589 1:-79606146 MsaT005265.1:CDS
AGTTACTTCTTCTTTGTTGA+TGG 0.359122 1:+79606979 None:intergenic
GTTCTATCACCTGGAGTGTC+AGG 0.361997 1:-79605225 MsaT005265.1:CDS
AGGTTCACCAATACTTGTTT+TGG 0.366406 1:+79602888 None:intergenic
CAATGCTACTTTGATCCGTT+TGG 0.373909 1:+79601930 None:intergenic
ATAACACATGGTCGTGATCT+TGG 0.375211 1:-79602446 MsaT005265.1:CDS
TAATTGCGACCAAAATAATA+TGG 0.382978 1:-79602256 MsaT005265.1:CDS
AAGAGATGGGTGACCTCATT+AGG 0.385790 1:-79603908 MsaT005265.1:CDS
CAGTGGGATGTTCTTTCCGT+TGG 0.395595 1:-79602494 MsaT005265.1:CDS
GTTCTCAATTGTGCCCTATC+GGG 0.401218 1:+79602093 None:intergenic
ATGTACTACTACTTGGTTGC+AGG 0.403605 1:+79604282 None:intergenic
ACAATACCAGTAGGTGTTCC+AGG 0.410735 1:+79602868 None:intergenic
TTTGTTGCTTTGCTTCCCTC+TGG 0.413727 1:-79602129 MsaT005265.1:CDS
AACAAAGAAGAAGTAACTTT+CGG 0.420411 1:-79606974 MsaT005265.1:CDS
TTCTGAATCTTCAAGAGAAT+CGG 0.421428 1:-79605495 MsaT005265.1:CDS
TCATTGGTGGAAATCCATAA+AGG 0.424927 1:+79605587 None:intergenic
TGTGATGGCAATGACCCCTT+AGG 0.427537 1:-79605707 MsaT005265.1:CDS
CATACCTTTGAACAACCATT+TGG 0.429043 1:+79604218 None:intergenic
AGTGGGATGTTCTTTCCGTT+GGG 0.429047 1:-79602493 MsaT005265.1:CDS
GGCAGTGGTTGATGTTTCCT+TGG 0.429868 1:-79604309 MsaT005265.1:CDS
CGTGTCAATGTACTACTACT+TGG 0.431548 1:+79604275 None:intergenic
TCTGTGAATGCAATCTTAAA+TGG 0.432736 1:-79602165 MsaT005265.1:CDS
TAACATCTATTTAGGTTACA+TGG 0.434505 1:-79604018 MsaT005265.1:intron
CCCAAAAGTCCGAAACTCAC+GGG 0.438000 1:-79605524 MsaT005265.1:CDS
GCTGAAAATGAGATCTATAA+GGG 0.438376 1:-79606145 MsaT005265.1:CDS
AAAAGCCTCCTTGGAACAAC+CGG 0.439163 1:+79602817 None:intergenic
GGTTCTCAATTGTGCCCTAT+CGG 0.442618 1:+79602092 None:intergenic
GTTTAGAATCTCAGTGTCAT+TGG 0.442788 1:+79605571 None:intergenic
ATCAACATCACTTCTTCAAT+CGG 0.448285 1:-79607080 MsaT005265.1:CDS
GAGCAATGCCCTCTCCCTAA+TGG 0.448625 1:-79606802 MsaT005265.1:CDS
TTTCCTTTGGAGCTAAGAGA+TGG 0.449285 1:-79603922 MsaT005265.1:CDS
ATGCTTAATTATGTTGAACT+TGG 0.451961 1:-79602060 MsaT005265.1:CDS
CATTGGTGGAAATCCATAAA+GGG 0.456139 1:+79605588 None:intergenic
TACTTCTTCTTTGTTGATGG+TGG 0.456523 1:+79606982 None:intergenic
GTCTTCTACAGGTATGTTGT+TGG 0.464682 1:+79603853 None:intergenic
ATGGGTAGAGCATGTAGAAC+TGG 0.464708 1:-79603999 MsaT005265.1:CDS
GGTGAACCTGGAACACCTAC+TGG 0.466104 1:-79602874 MsaT005265.1:CDS
CTTAATTATGTTGAACTTGG+AGG 0.468920 1:-79602057 MsaT005265.1:CDS
TCTACACCTGCATTTGAAAT+GGG 0.468937 1:-79605773 MsaT005265.1:CDS
ACTGGTATTGTTCTTAGTGT+TGG 0.469493 1:-79602856 MsaT005265.1:CDS
CTCACATGTTATTTATGCTC+CGG 0.470973 1:-79602199 MsaT005265.1:CDS
CAAAATCATTAGGAATTGAG+AGG 0.481538 1:+79605681 None:intergenic
AGTAAACGATGTAGATCTAG+TGG 0.483303 1:-79605822 MsaT005265.1:CDS
CTTTGCTTGCCATTAGGGAG+AGG 0.485297 1:+79606793 None:intergenic
ATTGAAGAAGTGATGTTGAT+TGG 0.487313 1:+79607083 None:intergenic
GCATGTCTCGAAATTGTTGT+TGG 0.489021 1:+79607105 None:intergenic
TCCTTCTGTAGCTAGTTGCT+GGG 0.489084 1:-79602950 MsaT005265.1:CDS
TTGTATTTCCCTGACACTCC+AGG 0.491222 1:+79605216 None:intergenic
TTCACAGAACCAATATCAAC+CGG 0.493030 1:+79602180 None:intergenic
TGTTGTTGGTGTACTGATTG+AGG 0.500312 1:+79607119 None:intergenic
TTTGCTTGCCATTAGGGAGA+GGG 0.507766 1:+79606794 None:intergenic
CACCTACGCAAATCAAGTCC+TGG 0.510955 1:-79606741 MsaT005265.1:CDS
TTAATTCAAGAACTACCAAA+TGG 0.513476 1:-79604233 MsaT005265.1:CDS
GAAGCACCAAGAAGGGATAT+GGG 0.516565 1:-79604330 MsaT005265.1:CDS
TTTGGCTTCCGGTTGTTCCA+AGG 0.518609 1:-79602825 MsaT005265.1:CDS
AGGAATTGAGAGGGACCCTA+AGG 0.522532 1:+79605691 None:intergenic
AAACAATTGAGTTGTGAGCT+AGG 0.524320 1:-79606769 MsaT005265.1:CDS
CCACTTCCCATTTCAAATGC+AGG 0.528835 1:+79605767 None:intergenic
TCCCAAAAGTCCGAAACTCA+CGG 0.534087 1:-79605525 MsaT005265.1:CDS
TAAACGAAGTTGAATACCCT+AGG 0.535905 1:-79605553 MsaT005265.1:CDS
TCTTAGATTGAAATGTTCAC+AGG 0.536806 1:-79605851 MsaT005265.1:intron
GTGGGATGTTCTTTCCGTTG+GGG 0.539001 1:-79602492 MsaT005265.1:CDS
ATACCACAATGGTCTTCTAC+AGG 0.539259 1:+79603842 None:intergenic
GTGATGGCAATGACCCCTTA+GGG 0.541659 1:-79605706 MsaT005265.1:CDS
TGTTACTCACCACATCCATA+AGG 0.542549 1:+79605435 None:intergenic
GTCATGACCAAAACAAGTAT+TGG 0.543952 1:-79602895 MsaT005265.1:CDS
AATTGAAAGGAAAATGGTTG+AGG 0.544455 1:-79607183 None:intergenic
TTCCTTTGGAGCTAAGAGAT+GGG 0.547566 1:-79603921 MsaT005265.1:CDS
TATTCTAAGAAGCACCAAGA+AGG 0.548624 1:-79604338 MsaT005265.1:CDS
GAATGCTAGATTAAAGGTTG+AGG 0.549279 1:-79606035 MsaT005265.1:intron
CTGAAAATGAGATCTATAAG+GGG 0.550420 1:-79606144 MsaT005265.1:CDS
ATCTGATTGTGACGATGTAA+GGG 0.553401 1:-79602917 MsaT005265.1:CDS
CACCCATCTCTTAGCTCCAA+AGG 0.553801 1:+79603919 None:intergenic
TTGACACGAAGACGACCCTC+TGG 0.556162 1:-79604260 MsaT005265.1:CDS
AAAACAAGTATTGGTGAACC+TGG 0.558177 1:-79602886 MsaT005265.1:CDS
CGAGCAGGAACCAGTGGTGA+AGG 0.561584 1:+79604380 None:intergenic
ACACTTGAAGTTCTATCACC+TGG 0.562131 1:-79605234 MsaT005265.1:CDS
TATATGCATGCAGACACAAC+AGG 0.563812 1:-79606215 MsaT005265.1:intron
CCTGCATTTGAAATGGGAAG+TGG 0.564061 1:-79605767 MsaT005265.1:CDS
AACGATTGTAGAACTTGCTG+TGG 0.564853 1:-79605651 MsaT005265.1:CDS
GGTTAGGATCTTGGTCTCGA+AGG 0.567881 1:+79607003 None:intergenic
CATTGATATCCTTATGGATG+TGG 0.570663 1:-79605444 MsaT005265.1:intron
CAAACGGATCAAAGTAGCAT+TGG 0.575903 1:-79601929 MsaT005265.1:CDS
TAGAATCTCAGTGTCATTGG+TGG 0.577352 1:+79605574 None:intergenic
CCAAGAAGGGATATGGGCAG+TGG 0.578162 1:-79604324 MsaT005265.1:CDS
ATTCTAAGAAGCACCAAGAA+GGG 0.578884 1:-79604337 MsaT005265.1:CDS
TGGGATGTTCTTTCCGTTGG+GGG 0.579262 1:-79602491 MsaT005265.1:CDS
TTCTCAATTGTGCCCTATCG+GGG 0.581221 1:+79602094 None:intergenic
ACTTGCTGTGGTTTCTATGG+AGG 0.581567 1:-79605639 MsaT005265.1:CDS
TTCACATTGTCTACCTAATG+AGG 0.582090 1:+79603895 None:intergenic
AAACGAAGTTGAATACCCTA+GGG 0.587167 1:-79605552 MsaT005265.1:CDS
TAGTTAAGAACACAACCAAA+CGG 0.589021 1:-79601945 MsaT005265.1:CDS
GTTAGGATCTTGGTCTCGAA+GGG 0.591139 1:+79607004 None:intergenic
AACAATGTCACACCAAAATG+TGG 0.593921 1:-79607150 MsaT005265.1:CDS
AACATCTATTTAGGTTACAT+GGG 0.594451 1:-79604017 MsaT005265.1:intron
ACTACCAAATGGTTGTTCAA+AGG 0.598187 1:-79604222 MsaT005265.1:intron
ACTTGGTTGCAGGTGATCCA+AGG 0.598845 1:+79604292 None:intergenic
TCTTGAATTAAACAACCAGA+GGG 0.610220 1:+79604245 None:intergenic
AGGGCACAATTGAGAACCAC+AGG 0.611548 1:-79602087 MsaT005265.1:CDS
CTCTCCCTAATGGCAAGCAA+AGG 0.612273 1:-79606792 MsaT005265.1:CDS
TTCTATCACCTGGAGTGTCA+GGG 0.612390 1:-79605224 MsaT005265.1:CDS
GGAATTGAGAGGGACCCTAA+GGG 0.613172 1:+79605692 None:intergenic
AGTTCAACATAATTAAGCAT+AGG 0.615403 1:+79602063 None:intergenic
TCCCAGCAACTAGCTACAGA+AGG 0.620988 1:+79602949 None:intergenic
AAAATCATTAGGAATTGAGA+GGG 0.623957 1:+79605682 None:intergenic
ACACTAAGAACAATACCAGT+AGG 0.625896 1:+79602859 None:intergenic
CCAAAAGTCCGAAACTCACG+GGG 0.627216 1:-79605523 MsaT005265.1:CDS
TTCTTGAATTAAACAACCAG+AGG 0.628816 1:+79604244 None:intergenic
ATAGAAGAATTGTATTGTGA+TGG 0.630421 1:-79605722 MsaT005265.1:CDS
GGCTTCCGGTTGTTCCAAGG+AGG 0.634589 1:-79602822 MsaT005265.1:CDS
ATAATTAAGCATAGGTCCTG+TGG 0.640980 1:+79602071 None:intergenic
GTAAACGATGTAGATCTAGT+GGG 0.648604 1:-79605821 MsaT005265.1:CDS
AAATCAAAATTCAAGAAGTG+AGG 0.648827 1:-79602782 MsaT005265.1:intron
GGAGTGTCAGGGAAATACAA+TGG 0.654696 1:-79605213 MsaT005265.1:CDS
ATTGGTGGAAATCCATAAAG+GGG 0.655135 1:+79605589 None:intergenic
TTGCGACCAAAATAATATGG+TGG 0.657066 1:-79602253 MsaT005265.1:CDS
GAATTGAGAGGGACCCTAAG+GGG 0.659587 1:+79605693 None:intergenic
AACCAGGACTTGATTTGCGT+AGG 0.662723 1:+79606739 None:intergenic
ATACAATGGAGCTCTACAAG+TGG 0.665675 1:-79605199 MsaT005265.1:intron
GGGGAGAATAACAAAACCAG+AGG 0.682229 1:+79602113 None:intergenic
ACTAGCTACAGAAGGACCAA+CGG 0.683763 1:+79602957 None:intergenic
GGGAGAATAACAAAACCAGA+GGG 0.693191 1:+79602114 None:intergenic
GAAATGGAACACATAACACA+TGG 0.699091 1:-79602458 MsaT005265.1:CDS
GTGAATGCAATCTTAAATGG+TGG 0.703104 1:-79602162 MsaT005265.1:CDS
TGAAAATGAGATCTATAAGG+GGG 0.713933 1:-79606143 MsaT005265.1:CDS
ATACCTGTAGAAGACCATTG+TGG 0.761535 1:-79603845 MsaT005265.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 79601912 79607192 79601912 ID=MsaG005265
Chr1 mRNA 79601912 79607192 79601912 ID=MsaT005265.1;Parent=MsaG005265
Chr1 exon 79601912 79602283 79601912 ID=MsaT005265.1.exon10;Parent=MsaT005265.1
Chr1 CDS 79601912 79602283 79601912 ID=cds.MsaT005265.1;Parent=MsaT005265.1
Chr1 exon 79602418 79602513 79602418 ID=MsaT005265.1.exon9;Parent=MsaT005265.1
Chr1 CDS 79602418 79602513 79602418 ID=cds.MsaT005265.1;Parent=MsaT005265.1
Chr1 exon 79602783 79602994 79602783 ID=MsaT005265.1.exon8;Parent=MsaT005265.1
Chr1 CDS 79602783 79602994 79602783 ID=cds.MsaT005265.1;Parent=MsaT005265.1
Chr1 exon 79603846 79604026 79603846 ID=MsaT005265.1.exon7;Parent=MsaT005265.1
Chr1 CDS 79603846 79604026 79603846 ID=cds.MsaT005265.1;Parent=MsaT005265.1
Chr1 exon 79604223 79604423 79604223 ID=MsaT005265.1.exon6;Parent=MsaT005265.1
Chr1 CDS 79604223 79604423 79604223 ID=cds.MsaT005265.1;Parent=MsaT005265.1
Chr1 exon 79605200 79605301 79605200 ID=MsaT005265.1.exon5;Parent=MsaT005265.1
Chr1 CDS 79605200 79605301 79605200 ID=cds.MsaT005265.1;Parent=MsaT005265.1
Chr1 exon 79605445 79605867 79605445 ID=MsaT005265.1.exon4;Parent=MsaT005265.1
Chr1 CDS 79605445 79605867 79605445 ID=cds.MsaT005265.1;Parent=MsaT005265.1
Chr1 exon 79606036 79606224 79606036 ID=MsaT005265.1.exon3;Parent=MsaT005265.1
Chr1 CDS 79606036 79606224 79606036 ID=cds.MsaT005265.1;Parent=MsaT005265.1
Chr1 exon 79606714 79606831 79606714 ID=MsaT005265.1.exon2;Parent=MsaT005265.1
Chr1 CDS 79606714 79606831 79606714 ID=cds.MsaT005265.1;Parent=MsaT005265.1
Chr1 exon 79606942 79607192 79606942 ID=MsaT005265.1.exon1;Parent=MsaT005265.1
Chr1 CDS 79606942 79607192 79606942 ID=cds.MsaT005265.1;Parent=MsaT005265.1
Gene Sequence

>MsaT005265.1

ATGGTTGAGGATGAACAACAAACAATGTCACACCAAAATGTGGTTGATCATCCTCAATCAGTACACCAACAACAATTTCGAGACATGCCAATCAACATCACTTCTTCAATCGGTTATTTGGAAAGATGTAATCAGAACCAAAATTCTAGAAATGAAGCTCTTTCTTCCCTTCGAGACCAAGATCCTAACCACCATCAACAAAGAAGAAGTAACTTTCGGCACTCGCAAAAACAGATTCAAGAAATGGAATCGTTCTTCGAGCAATGCCCTCTCCCTAATGGCAAGCAAAGGAAACAATTGAGTTGTGAGCTAGGTCTTACACCTACGCAAATCAAGTCCTGGTTTCAAAACAAGCGCAACCAAATTAAGACACAACAGGAACTTCACCAAAATGATCTTTTGAGGGACGAGAATGAGAAGCTTCGTGCTGAAAATGAGATCTATAAGGGGGCACTAAAAGACTCTTTATGTCCAAATTGTACTGATCCAATTTCTATTGGTGAAATGTCCTCTAACGACAATCAACTTAGAATTGAGAATGCTAGATTAAAGGTTGAGATTGAAATGTTCACAGGAAAGTTAGTAAACGATGTAGATCTAGTGGGTTCCTCCTCTAAAATTGATCAAATATCTACACCTGCATTTGAAATGGGAAGTGGTAGTGATATCTCTTCAAGCATGATAGAAGAATTGTATTGTGATGGCAATGACCCCTTAGGGTCCCTCTCAATTCCTAATGATTTTGACAAAGCAACGATTGTAGAACTTGCTGTGGTTTCTATGGAGGAACTCACAATGTTAACTCTTGCTGGATCCCCTTTATGGATTTCCACCAATGACACTGAGATTCTAAACGAAGTTGAATACCCTAGGGTTTTTCCCAAAAGTCCGAAACTCACGGGGTTAAATTCTGAATCTTCAAGAGAATCGGTTATTGTTTCCATGAATCATATTAATCTCATTGATATCCTTATGGATGTGAGTCAATGGTCAACTATGTTTCATGCGATTGTTTCAAAAGCATTGACACTTGAAGTTCTATCACCTGGAGTGTCAGGGAAATACAATGGAGCTCTACAAGTGATGTCAGCTGATTTTCAGGTTCCTTCACCACTGGTTCCTGCTCGTGAAAACTATTTCGTTAGATATTCTAAGAAGCACCAAGAAGGGATATGGGCAGTGGTTGATGTTTCCTTGGATCACCTGCAACCAAGTAGTAGTACATTGACACGAAGACGACCCTCTGGTTGTTTAATTCAAGAACTACCAAATGGTTGTTCAAAGGTTACATGGGTAGAGCATGTAGAACTGGATGAAAACACAATAGTGTCAAATCCATTCTATAAATCTTTAATAACTTCTGGTCTTTCCTTTGGAGCTAAGAGATGGGTGACCTCATTAGGTAGACAATGTGAACGTCTTGCTTGTTCAATGACCAACAACATACCTGTAGAAGACCATTGTGTATGTTTTTATGCTGCCGTTGGTCCTTCTGTAGCTAGTTGCTGGGAAGTGTTAACATCTGATTGTGACGATGTAAGGGTCATGACCAAAACAAGTATTGGTGAACCTGGAACACCTACTGGTATTGTTCTTAGTGTTGGAACTTCTATTTGGCTTCCGGTTGTTCCAAGGAGGCTTTTTGATTTTCTTGGAAATCAAAATTCAAGAAGTGAGTGGGATGTTCTTTCCGTTGGGGGTATAATTAAAGAAATGGAACACATAACACATGGTCGTGATCTTGGAAACTATGTCTCTTTATTTCATGTCAGCAGGGCTAATTGCGACCAAAATAATATGGTGGTGCTTCAAGACAATAACATTGATAGTACATGCTCACATGTTATTTATGCTCCGGTTGATATTGGTTCTGTGAATGCAATCTTAAATGGTGGTGATCCAAATTTTGTTGCTTTGCTTCCCTCTGGTTTTGTTATTCTCCCCGATAGGGCACAATTGAGAACCACAGGACCTATGCTTAATTATGTTGAACTTGGAGGTTGTCTATTGACAATTTCATTTCAATTTTTAGTTGATCCAAGTCCAACAAGAAGAGTAGAAAATGACTCAATAGAATTTGCTGTCAAATTAGTTAAGAACACAACCAAACGGATCAAAGTAGCATTGGTCGATAGCAACACCTAA
Protein sequence

>MsaT005265.1

MVEDEQQTMSHQNVVDHPQSVHQQQFRDMPINITSSIGYLERCNQNQNSRNEALSSLRDQDPNHHQQRRSNFRHSQKQIQEMESFFEQCPLPNGKQRKQLSCELGLTPTQIKSWFQNKRNQIKTQQELHQNDLLRDENEKLRAENEIYKGALKDSLCPNCTDPISIGEMSSNDNQLRIENARLKVEIEMFTGKLVNDVDLVGSSSKIDQISTPAFEMGSGSDISSSMIEELYCDGNDPLGSLSIPNDFDKATIVELAVVSMEELTMLTLAGSPLWISTNDTEILNEVEYPRVFPKSPKLTGLNSESSRESVIVSMNHINLIDILMDVSQWSTMFHAIVSKALTLEVLSPGVSGKYNGALQVMSADFQVPSPLVPARENYFVRYSKKHQEGIWAVVDVSLDHLQPSSSTLTRRRPSGCLIQELPNGCSKVTWVEHVELDENTIVSNPFYKSLITSGLSFGAKRWVTSLGRQCERLACSMTNNIPVEDHCVCFYAAVGPSVASCWEVLTSDCDDVRVMTKTSIGEPGTPTGIVLSVGTSIWLPVVPRRLFDFLGNQNSRSEWDVLSVGGIIKEMEHITHGRDLGNYVSLFHVSRANCDQNNMVVLQDNNIDSTCSHVIYAPVDIGSVNAILNGGDPNFVALLPSGFVILPDRAQLRTTGPMLNYVELGGCLLTISFQFLVDPSPTRRVENDSIEFAVKLVKNTTKRIKVALVDSNT*