Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG005299 | RHN82128.1 | 73.666 | 581 | 122 | 5 | 500 | 1070 | 1 | 560 | 0.0 | 788 |
| MsaG005299 | RHN82128.1 | 82.044 | 1086 | 157 | 8 | 1 | 1070 | 139 | 1202 | 0.0 | 1734 |
| MsaG005299 | RHN82128.1 | 92.480 | 492 | 36 | 1 | 1 | 492 | 781 | 1271 | 0.0 | 917 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG005299 | sp|Q8S9L6|CRK29_ARATH | 54.000 | 500 | 190 | 9 | 19 | 486 | 157 | 648 | 3.29e-167 | 511 |
| MsaG005299 | sp|Q8S9L6|CRK29_ARATH | 47.563 | 595 | 266 | 14 | 506 | 1069 | 5 | 584 | 5.33e-159 | 490 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG005299 | tr|A0A396K263|A0A396K263_MEDTR | 73.666 | 581 | 122 | 5 | 500 | 1070 | 1 | 560 | 0.0 | 788 |
| MsaG005299 | tr|A0A396K263|A0A396K263_MEDTR | 82.044 | 1086 | 157 | 8 | 1 | 1070 | 139 | 1202 | 0.0 | 1734 |
| MsaG005299 | tr|A0A396K263|A0A396K263_MEDTR | 92.480 | 492 | 36 | 1 | 1 | 492 | 781 | 1271 | 0.0 | 917 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG005299 | MtrunA17_Chr1g0206521 | 82.044 | 1086 | 157 | 8 | 1 | 1070 | 139 | 1202 | 0.0 | 1734 |
| MsaG005299 | MtrunA17_Chr1g0206521 | 92.480 | 492 | 36 | 1 | 1 | 492 | 781 | 1271 | 0.0 | 917 |
| MsaG005299 | MtrunA17_Chr1g0206521 | 73.666 | 581 | 122 | 5 | 500 | 1070 | 1 | 560 | 0.0 | 788 |
| MsaG005299 | MtrunA17_Chr1g0206451 | 79.549 | 577 | 100 | 5 | 499 | 1070 | 34 | 597 | 0.0 | 883 |
| MsaG005299 | MtrunA17_Chr1g0206451 | 83.333 | 498 | 77 | 4 | 1 | 494 | 173 | 668 | 0.0 | 768 |
| MsaG005299 | MtrunA17_Chr1g0206461 | 87.652 | 494 | 59 | 2 | 1 | 494 | 138 | 629 | 0.0 | 863 |
| MsaG005299 | MtrunA17_Chr1g0206461 | 73.276 | 580 | 124 | 6 | 500 | 1070 | 1 | 558 | 0.0 | 845 |
| MsaG005299 | MtrunA17_Chr1g0206531 | 73.621 | 580 | 126 | 5 | 500 | 1070 | 1 | 562 | 0.0 | 845 |
| MsaG005299 | MtrunA17_Chr1g0206531 | 86.465 | 495 | 63 | 2 | 1 | 492 | 138 | 631 | 0.0 | 802 |
| MsaG005299 | MtrunA17_Chr1g0206561 | 55.092 | 599 | 239 | 7 | 508 | 1096 | 3 | 581 | 0.0 | 600 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG005299 | AT4G21410.1 | 53.924 | 497 | 195 | 8 | 19 | 486 | 32 | 523 | 3.87e-170 | 512 |
| MsaG005299 | AT4G21410.1 | 49.149 | 470 | 202 | 10 | 626 | 1069 | 1 | 459 | 5.07e-134 | 418 |
| MsaG005299 | AT4G21410.3 | 54.000 | 500 | 190 | 9 | 19 | 486 | 157 | 648 | 3.34e-168 | 511 |
| MsaG005299 | AT4G21410.3 | 47.563 | 595 | 266 | 14 | 506 | 1069 | 5 | 584 | 5.42e-160 | 490 |
| MsaG005299 | AT4G23230.1 | 51.633 | 490 | 220 | 7 | 6 | 483 | 138 | 622 | 2.06e-163 | 497 |
| MsaG005299 | AT4G23230.1 | 46.237 | 558 | 260 | 14 | 531 | 1070 | 26 | 561 | 6.93e-154 | 472 |
| MsaG005299 | AT4G23180.1 | 46.924 | 569 | 244 | 13 | 529 | 1071 | 37 | 573 | 3.07e-162 | 496 |
| MsaG005299 | AT4G23180.1 | 51.660 | 482 | 220 | 6 | 11 | 483 | 156 | 633 | 3.66e-161 | 493 |
| MsaG005299 | AT4G38830.1 | 47.586 | 580 | 260 | 10 | 526 | 1083 | 21 | 578 | 2.04e-161 | 493 |
| MsaG005299 | AT4G38830.1 | 51.324 | 491 | 216 | 8 | 13 | 483 | 145 | 632 | 2.98e-156 | 480 |
Find 174 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCTTCCCATCAGAAGA+AGG | 0.543138 | 1:-79921175 | MsaT005299.1:intron |
| AAAAGGTTGTCAACAAATTC+AGG | 0.393857 | 1:-79927340 | MsaT005299.1:CDS |
| AAAAGGTTGTCAAGAGATTC+TGG | 0.365595 | 1:-79895339 | MsaT005299.1:CDS |
| AAAATTCAAAGTTGCATTAC+CGG | 0.308779 | 1:+79928424 | None:intergenic |
| AAACAAAGAACGATTGGATT+GGG | 0.464657 | 1:-79927075 | MsaT005299.1:CDS |
| AAACTTGGACATGGCGGATT+TGG | 0.256425 | 1:-79927527 | MsaT005299.1:CDS |
| AAAGAAAGAACAATTAGATT+GGG | 0.474755 | 1:-79895040 | MsaT005299.1:CDS |
| AAAGCTTGTTGCGTTCTTCA+TGG | 0.383255 | 1:-79926435 | MsaT005299.1:CDS |
| AACAACAGTCGGCACAACTA+TGG | 0.428354 | 1:+79927981 | None:intergenic |
| AACGCCATTGGACTCTGCAG+AGG | 0.649863 | 1:-79921706 | MsaT005299.1:CDS |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 79889976 | 79928585 | 79889976 | ID=MsaG005299 |
| Chr1 | mRNA | 79889976 | 79928585 | 79889976 | ID=MsaT005299.1;Parent=MsaG005299 |
| Chr1 | exon | 79889976 | 79890064 | 79889976 | ID=MsaT005299.1.exon15;Parent=MsaT005299.1 |
| Chr1 | CDS | 79889976 | 79890064 | 79889976 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79894230 | 79894251 | 79894230 | ID=MsaT005299.1.exon14;Parent=MsaT005299.1 |
| Chr1 | CDS | 79894230 | 79894251 | 79894230 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79894390 | 79894540 | 79894390 | ID=MsaT005299.1.exon13;Parent=MsaT005299.1 |
| Chr1 | CDS | 79894390 | 79894540 | 79894390 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79894832 | 79895069 | 79894832 | ID=MsaT005299.1.exon12;Parent=MsaT005299.1 |
| Chr1 | CDS | 79894832 | 79895069 | 79894832 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79895184 | 79895388 | 79895184 | ID=MsaT005299.1.exon11;Parent=MsaT005299.1 |
| Chr1 | CDS | 79895184 | 79895388 | 79895184 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79895476 | 79895689 | 79895476 | ID=MsaT005299.1.exon10;Parent=MsaT005299.1 |
| Chr1 | CDS | 79895476 | 79895689 | 79895476 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79909946 | 79910024 | 79909946 | ID=MsaT005299.1.exon9;Parent=MsaT005299.1 |
| Chr1 | CDS | 79909946 | 79910024 | 79909946 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79921176 | 79921992 | 79921176 | ID=MsaT005299.1.exon8;Parent=MsaT005299.1 |
| Chr1 | CDS | 79921176 | 79921992 | 79921176 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79926038 | 79926274 | 79926038 | ID=MsaT005299.1.exon7;Parent=MsaT005299.1 |
| Chr1 | CDS | 79926038 | 79926274 | 79926038 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79926407 | 79926557 | 79926407 | ID=MsaT005299.1.exon6;Parent=MsaT005299.1 |
| Chr1 | CDS | 79926407 | 79926557 | 79926407 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79926867 | 79927104 | 79926867 | ID=MsaT005299.1.exon5;Parent=MsaT005299.1 |
| Chr1 | CDS | 79926867 | 79927104 | 79926867 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79927185 | 79927395 | 79927185 | ID=MsaT005299.1.exon4;Parent=MsaT005299.1 |
| Chr1 | CDS | 79927185 | 79927395 | 79927185 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79927514 | 79927650 | 79927514 | ID=MsaT005299.1.exon3;Parent=MsaT005299.1 |
| Chr1 | CDS | 79927514 | 79927650 | 79927514 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79927908 | 79928033 | 79927908 | ID=MsaT005299.1.exon2;Parent=MsaT005299.1 |
| Chr1 | CDS | 79927908 | 79928033 | 79927908 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Chr1 | exon | 79928204 | 79928585 | 79928204 | ID=MsaT005299.1.exon1;Parent=MsaT005299.1 |
| Chr1 | CDS | 79928204 | 79928585 | 79928204 | ID=cds.MsaT005299.1;Parent=MsaT005299.1 |
| Gene Sequence |
>MsaT005299.1 ATGGATGCTTCATTTTCATATTATAAGTGGAACAATAGAAATGTAACCGATGTGGATCAATTTAATCAAGTTCTTGGGAACTTGATGAGGAAACTCAATGAAATAGCTGCATCGGGTGATTCTCGTCGTAAGTTTGCTACCGGTAATGCAACTTTGAATTTTGAAACCATATATGGTCTTGTGCAGTGTACACCTGACTTGTCAGGGCAAGATTGCAATGATTGTTTAGAGCGTGCTATTTCAGAAATTCCAAGTGTTTTTAAAAACAAGATTGGTGGTAGAGTTCTCAAACTAAGTTGTAATATTAGATATGAAATATATTCCTTCTATGGTCCTACGACAGTAATAGACCAAGATGAAACCTCCCACCAGAAGAAGGTAAAAAAAAACAAATCATCACATACTACCATCGCCATAGTTGTGCCGACTGTTGTTGTTGTTGTTGCTGCCCTGCTAACTTTCATTTGCATATGCTTAAGGAAAAGGAAGGCAAGGATAACACTCGAAGATATAGAAGAAGATGATAATAATGATATTGAAATCACTGAGTCATTGCTATTCAACTTCGAGACATTAAGAGTTGCTACAAGTAACTTTTCTGAAGCTAATAAACTTGGACATGGCGGATTTGGAGTTGTTTATCAGGGTATACTCTCTGGTGGACAAGTGATTGCGGTCAAAAGGTTGTCAACAAATTCAGGACAAGGGGATATTGAATTTAAGAATGAAGTGCTTTTAGTTGCTAAGCTTCAACATCGAAATTTAGTTCGACTACTTGGTTTTTGTCTAGAAGGAAGAGAAAGATTGCTTGTCTATGAATATGTTCCAAATAAAAGTCTTGATTATTTTATATTTGATCCAATAAACAAAGAACGATTGGATTGGGAAAGGCGCTACAAAATCATTGGAGGTATTGCTAGAGGCCTTCTTTACCTTCATCAGGATTCTCAACTTCGTATTATACATCGTGATCTTAAAGCAAGTAACATTCTCTTGGACGAAAAGTTGAATCCTAAAATATCAGATTTTGGTATCGCCAGACTACTTCTTGTCGATCAAACTCAAGTTAATACAAATAAAGTCGTTGGAACATATGGATATATGGCTCCTGAGTATGCAATGTTTGGAGAATTTTCGGTAAAATCGGATGTTTTTAGTTTTGGTGTACTAGTTTTGGAGATTATAAGTGGACAGAAAGCTTGTTGCGTTCTTCATGGGCAGAGTCAGGAGGATTTACTTAGCTTTGCATGGAGAAACTGGAGGGAGGGTACAATCACAAATATAATAGATCCATCATTAAGCAATGGTTCACAAAATGAAATCATGAGATGCATTCACATTGCTTTACTATGTGTTCAAGAAAATTTGGTTGAAAGACCAACAATGGCAACCGTTGTACTCATGCTTAGTAGTTATTCTATCTCTCTCTCTGTACCTTTGGAGCCTGCATCTATTGTGGGAGGCACAACTAGAAGTCATCCAAGATCATTAACACGAATGGAAATTGTTTCTAGCAAGCTATTATTTTCATTTTATTCTTTTCTCCATTTTATTATCATTATCGTATCTCAAGCCCAAGGTCAACCAGATTTTGTGCGCTACGGTTGTTCAAACAATAATGGCAACTACACAGCCAATAGCACCTATCAAAACAACCTCAACACCCTATTATCCAATCTCACTTCCAACGAACAAATCAACTACGGTTTCTACAATCTCTCTTATGGCCAAAACACCGACAAAGTAAACGCCATTGGACTCTGCAGAGGAGATGTTAATCCTGACGTTTGCCGCAGTTGTCTCAATGATTCAAGAGTTCTTCTCACAAAACTTTGTCCAAACCAAAAAGAAGCAATTGGTTGGTATGAGGACTGCATGTTGCGTTACTCAAACCGATCAATATTTGGTATTCTTGAATGGTCACCAAAGTTTTATATGGCGAACCCGAACAATGTAACCGAGGTGGATCTATTTAATCGAGTTCTTGGAAACTTGATGAGGAAACTGAAAGAAATAGCTGCCTCAAGTGATTCTCGTCTTAAGTTTGCTGCGGATAATGCAACTGATGTGAATTTGAATTTTCAAACCATATATGGTCTTGTGCAGTGTACACCTGATTTGTCAGAGCAAGATTGCAATGATTGTTTAGAGTTCGCTATATCAGAAATTCCAATTTTTTGTAATAACAAGATTGGTTGTAGAGTTGTCAAACCAAGTTGTAATATTAGATATGAAGTCTATCGCTTCTCTAATTCTACGACAGTAATAGACCAAGATAAAACCTTCCCATCAGAAGAAGTGCCCTTTTTCTTGGAAATAATGGTAATGCCAGAAAAAACCAAAACATTTGTTATTTTTCTTATTAATTTCAAAAATCTTGTTTTGATTTCAAAAATTCGATATAAGGATAATTTTCTATATGTTGGCTTCACTGACATGTCTTGTATAATGTTTGAAGAAATAGAAGATGACAGTGACTATATTGAAATTTCCGAGTCATTGCAATTCAACTTCGACACAATACAAGTTGCTACAAGTAACTTTTCTGATGCTAATGAACTTGGATGTGGAGGATTTGGAGTTGTTTATCAGGCTCCCAATGGACAAGTGATTGCCATCAAAAGGTTGTCAAGAGATTCTGGACAGGGTGATATTGAATTTAAGAATGAAGTGCAATTAGTGGCTAAGCTTCAACACCGAAATTTAGTTAGACTAGTTGGTTTTTGTCTCGAAGGAAGAGAAAGACTACTTGTCTATGAATTTGTTCCCAATAAAAGCCTTGATTACTTTATATTTGATCCAATAAAGAAAGAACAATTAGATTGGGAAAGGCGCTACAAAATCATTGAAGGTATTGTTAGAGGCCTTCTTTACCTTCATCAAGATTCTCAACTTCGCATTATACATCGTGATCTTAAAGCAAGTAACATTCTCTTGGACGAGAAGATGAATCCTAAAATATCAGATTTTGGTATGGCCAGACTAATTCTTGTGGATCAAACTCAAGTAAATACAGATAAAGTCGTTGGAACATATGGATACATGGCACCCAAATATGCAATGTTTGGACAGTTTTCGGTCAAATCGGATGTTTTTAGTTTTGGCGTACTAGTTCTAGAGATTATAAGTGGGCAAAAGATTAGTGGTATTCTTCATGGACAGAGTCAGGAGGATTTACTTAGCTTTGCATGGAGAAACTGGAGGGAAGGTGAAAATTTCTTTGTGGCTGCTTCAATAGTCACCAAATTATCTGCAGCGATTGTAACACAGCACTTTCATCACCAACTGGAGGAATAA |
| Protein sequence |
>MsaT005299.1 MDASFSYYKWNNRNVTDVDQFNQVLGNLMRKLNEIAASGDSRRKFATGNATLNFETIYGLVQCTPDLSGQDCNDCLERAISEIPSVFKNKIGGRVLKLSCNIRYEIYSFYGPTTVIDQDETSHQKKVKKNKSSHTTIAIVVPTVVVVVAALLTFICICLRKRKARITLEDIEEDDNNDIEITESLLFNFETLRVATSNFSEANKLGHGGFGVVYQGILSGGQVIAVKRLSTNSGQGDIEFKNEVLLVAKLQHRNLVRLLGFCLEGRERLLVYEYVPNKSLDYFIFDPINKERLDWERRYKIIGGIARGLLYLHQDSQLRIIHRDLKASNILLDEKLNPKISDFGIARLLLVDQTQVNTNKVVGTYGYMAPEYAMFGEFSVKSDVFSFGVLVLEIISGQKACCVLHGQSQEDLLSFAWRNWREGTITNIIDPSLSNGSQNEIMRCIHIALLCVQENLVERPTMATVVLMLSSYSISLSVPLEPASIVGGTTRSHPRSLTRMEIVSSKLLFSFYSFLHFIIIIVSQAQGQPDFVRYGCSNNNGNYTANSTYQNNLNTLLSNLTSNEQINYGFYNLSYGQNTDKVNAIGLCRGDVNPDVCRSCLNDSRVLLTKLCPNQKEAIGWYEDCMLRYSNRSIFGILEWSPKFYMANPNNVTEVDLFNRVLGNLMRKLKEIAASSDSRLKFAADNATDVNLNFQTIYGLVQCTPDLSEQDCNDCLEFAISEIPIFCNNKIGCRVVKPSCNIRYEVYRFSNSTTVIDQDKTFPSEEVPFFLEIMVMPEKTKTFVIFLINFKNLVLISKIRYKDNFLYVGFTDMSCIMFEEIEDDSDYIEISESLQFNFDTIQVATSNFSDANELGCGGFGVVYQAPNGQVIAIKRLSRDSGQGDIEFKNEVQLVAKLQHRNLVRLVGFCLEGRERLLVYEFVPNKSLDYFIFDPIKKEQLDWERRYKIIEGIVRGLLYLHQDSQLRIIHRDLKASNILLDEKMNPKISDFGMARLILVDQTQVNTDKVVGTYGYMAPKYAMFGQFSVKSDVFSFGVLVLEIISGQKISGILHGQSQEDLLSFAWRNWREGENFFVAASIVTKLSAAIVTQHFHHQLEE* |
