AlfalfaGEDB | MsaG005320

M. sativa ssp. caerulea / MsaG005320

KEGG: K11644
GO: GO:0000118 GO:0000122 GO:0000785 GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0033993 GO:0035601 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0097305 GO:0098732 GO:1901564 GO:1901700 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005320	KEH43987.1	62.121	66	25	0	14	79	93	158	1.68e-15	79.7
MsaG005320	KEH43987.1	91.150	113	10	0	1	113	1	113	1.44e-68	214

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005320	sp\|O48686\|SNL3_ARATH	49.524	105	42	2	13	108	11	113	1.99e-25	103

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005320	tr\|A0A072VQC1\|A0A072VQC1_MEDTR	62.121	66	25	0	14	79	93	158	8.03e-16	79.7
MsaG005320	tr\|A0A072VQC1\|A0A072VQC1_MEDTR	91.150	113	10	0	1	113	1	113	6.85e-69	214

TFs/TRs:

Gene ID	Type	Classification
MsaG005320	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005320	MtrunA17_Chr1g0206771	91.150	113	10	0	1	113	1	113	1.32e-72	214
MsaG005320	MtrunA17_Chr1g0206771	62.121	66	25	0	14	79	93	158	1.55e-19	79.7
MsaG005320	MtrunA17_Chr1g0206781	63.158	76	28	0	13	88	8	83	1.34e-29	103
MsaG005320	MtrunA17_Chr5g0415031	52.427	103	42	2	13	108	40	142	5.64e-27	104
MsaG005320	MtrunA17_Chr8g0364231	49.091	110	49	1	6	108	32	141	5.09e-26	102
MsaG005320	MtrunA17_Chr6g0452001	50.485	103	44	2	13	108	60	162	1.05e-24	98.2
MsaG005320	MtrunA17_Chr6g0452021	47.573	103	49	1	13	110	40	142	5.02e-22	90.1
MsaG005320	MtrunA17_Chr1g0205861	50.617	81	40	0	1	81	1	81	2.49e-21	82.8
MsaG005320	MtrunA17_Chr7g0256011	50.649	77	38	0	7	83	7	83	6.16e-21	81.6
MsaG005320	MtrunA17_Chr3g0077911	58.209	67	27	1	14	79	88	154	7.02e-20	80.1
MsaG005320	MtrunA17_Chr3g0077911	48.485	66	31	1	13	78	5	67	1.89e-12	60.8
MsaG005320	MtrunA17_Chr3g0077861	58.209	67	27	1	14	79	88	154	1.02e-19	79.7
MsaG005320	MtrunA17_Chr1g0205811	52.055	73	35	0	16	88	14	86	2.52e-18	75.1
MsaG005320	MtrunA17_Chr8g0364021	38.835	103	57	2	9	106	37	138	3.81e-17	74.7
MsaG005320	MtrunA17_Chr3g0077891	50.000	74	36	1	7	79	90	163	4.95e-17	73.2
MsaG005320	MtrunA17_Chr1g0209201	43.750	96	47	3	19	112	19	109	3.97e-16	70.1
MsaG005320	MtrunA17_Chr3g0077941	46.988	83	39	2	8	89	90	168	1.28e-15	69.3
MsaG005320	MtrunA17_Chr7g0218911	49.180	61	31	0	20	80	2	62	3.26e-15	67.0
MsaG005320	MtrunA17_Chr4g0075861	55.738	61	27	0	19	79	175	235	3.74e-15	70.5
MsaG005320	MtrunA17_Chr4g0075861	42.000	100	54	2	15	113	11	107	5.29e-13	64.3
MsaG005320	MtrunA17_Chr4g0075861	41.096	73	43	0	15	87	88	160	3.12e-11	59.3
MsaG005320	MtrunA17_Chr4g0075971	55.738	61	27	0	19	79	175	235	4.97e-15	70.5
MsaG005320	MtrunA17_Chr4g0075971	41.176	102	52	3	15	113	11	107	5.22e-13	64.7
MsaG005320	MtrunA17_Chr1g0205821	37.209	86	53	1	4	88	86	171	1.07e-14	67.0
MsaG005320	MtrunA17_Chr1g0205821	34.483	116	69	1	5	113	2	117	2.04e-13	63.5
MsaG005320	MtrunA17_Chr1g0205801	43.836	73	41	0	16	88	12	84	3.06e-14	64.3
MsaG005320	MtrunA17_Chr2g0328701	40.196	102	53	3	15	113	28	124	9.95e-14	63.2
MsaG005320	MtrunA17_Chr3g0077881	42.857	91	47	2	5	94	88	174	1.11e-13	64.3
MsaG005320	MtrunA17_Chr1g0156171	62.857	35	13	0	46	80	17	51	7.90e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005320	AT1G24190.3	49.524	105	42	2	13	108	11	113	2.01e-26	103
MsaG005320	AT1G24190.2	49.524	105	42	2	13	108	11	113	2.03e-26	103
MsaG005320	AT1G24190.1	49.524	105	42	2	13	108	11	113	2.03e-26	103
MsaG005320	AT5G15020.1	50.000	104	43	2	13	109	49	150	3.39e-26	102
MsaG005320	AT5G15020.2	50.000	104	43	2	13	109	49	150	3.70e-26	102
MsaG005320	AT1G70060.2	50.000	106	41	2	13	108	11	114	1.78e-25	100
MsaG005320	AT1G70060.1	50.000	106	41	2	13	108	11	114	1.78e-25	100
MsaG005320	AT3G01320.1	48.077	104	48	2	13	110	54	157	3.74e-24	97.1
MsaG005320	AT3G01320.2	48.077	104	48	2	13	110	54	157	4.00e-24	97.1
MsaG005320	AT1G59890.3	63.235	68	25	0	16	83	45	112	1.66e-23	95.1
MsaG005320	AT1G59890.2	63.235	68	25	0	16	83	45	112	1.68e-23	95.1
MsaG005320	AT1G59890.1	63.235	68	25	0	16	83	45	112	1.74e-23	95.1
MsaG005320	AT1G59890.4	63.235	68	25	0	16	83	45	112	1.92e-23	95.1
MsaG005320	AT1G24200.1	43.564	101	53	1	12	108	10	110	1.10e-22	88.6
MsaG005320	AT1G10450.3	53.750	80	34	1	7	83	72	151	3.09e-20	85.9
MsaG005320	AT1G10450.2	53.750	80	34	1	7	83	21	100	3.43e-20	85.9
MsaG005320	AT1G10450.1	53.750	80	34	1	7	83	21	100	3.43e-20	85.9
MsaG005320	AT4G12020.11	59.701	67	26	1	13	79	305	370	1.05e-19	84.3
MsaG005320	AT4G12020.6	59.701	67	26	1	13	79	305	370	1.07e-19	84.3
MsaG005320	AT4G12020.8	59.701	67	26	1	13	79	305	370	1.07e-19	84.3
MsaG005320	AT4G12020.7	59.701	67	26	1	13	79	305	370	1.07e-19	84.3
MsaG005320	AT4G12020.1	59.701	67	26	1	13	79	305	370	1.07e-19	84.3
MsaG005320	AT4G12020.9	59.701	67	26	1	13	79	305	370	1.07e-19	84.3
MsaG005320	AT4G12020.5	59.701	67	26	1	13	79	238	303	1.07e-19	84.3
MsaG005320	AT4G12020.10	59.701	67	26	1	13	79	305	370	1.08e-19	84.3
MsaG005320	AT4G12020.3	59.701	67	26	1	13	79	305	370	1.08e-19	84.3
MsaG005320	AT4G12020.4	59.701	67	26	1	13	79	305	370	1.08e-19	84.3
MsaG005320	AT4G12020.2	59.701	67	26	1	13	79	305	370	1.08e-19	84.3
MsaG005320	AT5G15025.1	55.882	68	30	0	13	80	44	111	1.20e-19	79.0
MsaG005320	AT5G15040.1	52.174	69	33	0	14	82	1	69	1.61e-18	75.1
MsaG005320	AT5G15040.3	52.174	69	33	0	14	82	1	69	1.61e-18	75.1
MsaG005320	AT5G15040.2	52.174	69	33	0	14	82	1	69	1.61e-18	75.1
MsaG005320	AT1G70030.1	38.235	102	57	1	13	108	8	109	6.99e-18	75.5
MsaG005320	AT1G27250.1	43.750	80	43	1	2	79	5	84	1.19e-17	73.9
MsaG005320	AT1G70030.2	37.963	108	59	2	13	112	19	126	4.03e-17	72.8
MsaG005320	AT1G70030.3	38.384	99	55	1	13	105	19	117	4.24e-17	72.4
MsaG005320	AT1G24230.1	35.294	102	57	2	13	113	11	104	5.17e-17	74.7
MsaG005320	AT1G24230.1	35.294	68	44	0	13	80	83	150	2.61e-12	62.0
MsaG005320	AT1G27220.1	46.575	73	37	1	9	79	5	77	8.63e-17	73.2
MsaG005320	AT1G23810.1	43.056	72	41	0	12	83	105	176	1.70e-16	73.2
MsaG005320	AT1G24250.1	44.444	72	40	0	12	83	116	187	4.66e-16	72.4
MsaG005320	AT1G27260.1	35.398	113	58	2	14	113	1	111	2.35e-15	70.1
MsaG005320	AT1G27260.1	36.585	82	49	1	5	83	79	160	1.52e-11	59.7
MsaG005320	AT1G27240.1	39.437	71	43	0	12	82	10	80	2.86e-13	63.9
MsaG005320	AT1G24210.1	39.726	73	44	0	13	85	11	83	3.87e-12	60.1
MsaG005320	AT1G24220.2	32.743	113	69	3	7	117	5	112	4.93e-12	62.4
MsaG005320	AT1G24220.1	32.743	113	69	3	7	117	5	112	4.93e-12	62.4
MsaG005320	AT1G27280.1	37.143	70	44	0	13	82	78	147	9.06e-12	60.5
MsaG005320	AT5G35610.1	41.333	75	43	1	6	80	1	74	6.04e-11	57.0
MsaG005320	AT1G27270.1	45.205	73	40	0	11	83	108	180	6.67e-11	58.2

Find 22 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATTGAAGGATTTGTTTAA+AGG	0.287517	1:+80165398	MsaT005320.1:CDS
TAATCAAACTTGCTCCCTTT+TGG	0.325065	1:-80165458	None:intergenic
AAAGGGAGCAAGTTTGATTA+TGG	0.329129	1:+80165461	MsaT005320.1:CDS
GATTGAAGGATTTGTTTAAA+GGG	0.359025	1:+80165399	MsaT005320.1:CDS
TGTTTCAATCTCAGGAAGAT+TGG	0.378188	1:+80166991	MsaT005320.1:intron
AAATGTTATGATGGATTTCA+AGG	0.397261	1:+80165343	MsaT005320.1:CDS
GCTTCTTCCAACACTCCAAA+TGG	0.411742	1:-80165491	None:intergenic
GATTATGGCATAGATCCATT+TGG	0.439528	1:+80165476	MsaT005320.1:CDS
TTCAACATCTTCGTGCCAAA+AGG	0.444915	1:+80165443	MsaT005320.1:CDS
CATAGATCCATTTGGAGTGT+TGG	0.482305	1:+80165484	MsaT005320.1:CDS
TTCACAGCAGCAAACTATCC+AGG	0.527716	1:+80167031	MsaT005320.1:CDS
TCAACATCTTCGTGCCAAAA+GGG	0.534332	1:+80165444	MsaT005320.1:CDS
TCCATGAGACATGTTATCGC+TGG	0.536428	1:-80165231	None:intergenic
TGGTGTCATAGCAAGATTGA+AGG	0.543726	1:+80165385	MsaT005320.1:CDS
TCCAGCGATAACATGTCTCA+TGG	0.552003	1:+80165230	MsaT005320.1:CDS
AGTTGAGTTTCAATATGAGA+GGG	0.554210	1:+80165538	MsaT005320.1:CDS
GCTATTGCTTCTTCCATCGT+GGG	0.554911	1:-80165260	None:intergenic
TGCTATTGCTTCTTCCATCG+TGG	0.562428	1:-80165261	None:intergenic
AAGTTGAGTTTCAATATGAG+AGG	0.581901	1:+80165537	MsaT005320.1:CDS
GCTATGAAAATTGATACAAG+TGG	0.667045	1:+80165365	MsaT005320.1:CDS
AGAAGCAATAGCATATGTCG+AGG	0.681954	1:+80165271	MsaT005320.1:CDS
TCATGGAGAAGTTCCCACGA+TGG	0.689488	1:+80165247	MsaT005320.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	80165227	80167071	80165227	ID=MsaG005320
Chr1	mRNA	80165227	80167071	80165227	ID=MsaT005320.1;Parent=MsaG005320
Chr1	exon	80165227	80165564	80165227	ID=MsaT005320.1.exon1;Parent=MsaT005320.1
Chr1	CDS	80165227	80165564	80165227	ID=cds.MsaT005320.1;Parent=MsaT005320.1
Chr1	exon	80167005	80167071	80167005	ID=MsaT005320.1.exon2;Parent=MsaT005320.1
Chr1	CDS	80167005	80167071	80167005	ID=cds.MsaT005320.1;Parent=MsaT005320.1

Gene Sequence

>MsaT005320.1

ATGTCCAGCGATAACATGTCTCATGGAGAAGTTCCCACGATGGAAGAAGCAATAGCATATGTCGAGGCTGTAAAAGACGAGTTTCAAGATGAGATAGAAAAGTATGATGAATTTTTAAATGTTATGATGGATTTCAAGGCTATGAAAATTGATACAAGTGGTGTCATAGCAAGATTGAAGGATTTGTTTAAAGGGCACACAAATTTAATTTTAGGATTCAACATCTTCGTGCCAAAAGGGAGCAAGTTTGATTATGGCATAGATCCATTTGGAGTGTTGGAAGAAGCAAAAGCATATGTCCATGCAGTAAAAGTTGAGTTTCAATATGAGAGGGAAAAGAAGATTGGTTGTTATTTACTGAACATTCACAGCAGCAAACTATCCAGGTTTTTCTATAGGTTGTAA

Protein sequence

>MsaT005320.1

MSSDNMSHGEVPTMEEAIAYVEAVKDEFQDEIEKYDEFLNVMMDFKAMKIDTSGVIARLKDLFKGHTNLILGFNIFVPKGSKFDYGIDPFGVLEEAKAYVHAVKVEFQYEREKKIGCYLLNIHSSKLSRFFYRL*