AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005321


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005321 MtrunA17_Chr1g0206781 94.118 119 7 0 1 119 1 119 1.44e-78 227
MsaG005321 MtrunA17_Chr1g0206771 52.066 121 39 1 8 109 13 133 1.77e-34 117
MsaG005321 MtrunA17_Chr1g0206771 63.380 71 26 0 10 80 94 164 1.44e-23 89.7
MsaG005321 MtrunA17_Chr8g0364231 68.605 86 27 0 1 86 32 117 1.07e-33 123
MsaG005321 MtrunA17_Chr5g0415031 63.529 85 31 0 4 88 36 120 3.41e-31 115
MsaG005321 MtrunA17_Chr6g0452001 64.286 84 30 0 3 86 55 138 4.41e-30 112
MsaG005321 MtrunA17_Chr1g0205811 58.974 78 32 0 12 89 15 92 1.46e-26 95.5
MsaG005321 MtrunA17_Chr6g0452021 57.647 85 34 1 2 86 34 116 5.86e-25 97.8
MsaG005321 MtrunA17_Chr1g0205821 52.703 74 35 0 12 85 100 173 4.91e-22 85.5
MsaG005321 MtrunA17_Chr1g0205821 37.190 121 50 1 12 106 14 134 7.70e-18 74.7
MsaG005321 MtrunA17_Chr3g0077861 59.459 74 29 1 4 76 83 156 4.30e-21 82.8
MsaG005321 MtrunA17_Chr3g0077861 40.164 122 44 3 12 107 9 127 4.59e-16 69.7
MsaG005321 MtrunA17_Chr3g0077911 59.459 74 29 1 4 76 83 156 7.36e-21 82.0
MsaG005321 MtrunA17_Chr3g0077911 40.984 122 43 2 12 107 9 127 9.73e-19 76.6
MsaG005321 MtrunA17_Chr8g0364021 53.247 77 36 0 3 79 36 112 4.09e-20 82.0
MsaG005321 MtrunA17_Chr3g0077891 51.807 83 39 1 4 85 92 174 7.17e-20 80.1
MsaG005321 MtrunA17_Chr3g0077891 37.209 129 49 2 5 101 2 130 2.59e-15 68.2
MsaG005321 MtrunA17_Chr1g0205801 50.000 76 38 0 12 87 13 88 3.07e-19 76.6
MsaG005321 MtrunA17_Chr1g0205861 42.045 88 51 0 2 89 7 94 3.60e-19 76.6
MsaG005321 MtrunA17_Chr7g0256011 51.429 70 34 0 12 81 17 86 5.10e-19 76.3
MsaG005321 MtrunA17_Chr1g0205831 47.436 78 41 0 14 91 76 153 9.07e-19 77.0
MsaG005321 MtrunA17_Chr1g0205831 39.604 101 50 2 13 102 4 104 1.09e-15 69.3
MsaG005321 MtrunA17_Chr1g0209201 39.286 112 50 2 14 108 19 129 3.97e-18 74.7
MsaG005321 MtrunA17_Chr7g0218911 55.385 65 29 0 15 79 2 66 7.40e-18 73.2
MsaG005321 MtrunA17_Chr4g0075971 56.061 66 29 0 14 79 175 240 9.00e-18 77.8
MsaG005321 MtrunA17_Chr4g0075971 40.870 115 45 2 12 106 13 124 3.08e-16 73.2
MsaG005321 MtrunA17_Chr4g0075971 36.441 118 52 1 12 106 90 207 5.63e-13 63.9
MsaG005321 MtrunA17_Chr4g0075861 56.061 66 29 0 14 79 175 240 1.02e-17 77.0
MsaG005321 MtrunA17_Chr4g0075861 40.870 115 45 2 12 106 13 124 9.12e-17 74.3
MsaG005321 MtrunA17_Chr4g0075861 37.288 118 51 1 12 106 90 207 2.72e-15 70.5
MsaG005321 MtrunA17_Chr2g0328701 44.944 89 44 2 12 98 30 115 8.10e-17 70.9
MsaG005321 MtrunA17_Chr3g0077941 48.750 80 40 1 7 85 94 173 2.61e-16 70.9
MsaG005321 MtrunA17_Chr3g0077941 37.209 129 50 2 5 102 2 130 6.67e-15 67.0
MsaG005321 MtrunA17_Chr1g0156171 71.795 39 11 0 41 79 17 55 4.63e-15 66.2
MsaG005321 MtrunA17_Chr3g0077881 36.434 129 50 2 5 101 2 130 9.54e-15 66.6
MsaG005321 MtrunA17_Chr3g0077881 46.250 80 42 1 4 82 92 171 2.25e-13 63.2
MsaG005321 MtrunA17_Chr5g0431721 43.820 89 45 4 1 84 1 89 4.43e-14 63.5
MsaG005321 MtrunA17_Chr3g0077811 48.000 75 38 1 5 78 7 81 1.64e-12 59.7
MsaG005321 MtrunA17_Chr7g0218891 36.047 86 53 1 3 86 177 262 3.60e-12 61.2
MsaG005321 MtrunA17_Chr7g0218891 30.303 132 64 4 3 106 5 136 8.47e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005321 AT1G24190.2 66.265 83 28 0 2 84 5 87 1.11e-31 117
MsaG005321 AT1G24190.3 66.265 83 28 0 2 84 5 87 1.12e-31 117
MsaG005321 AT1G24190.1 66.265 83 28 0 2 84 5 87 1.14e-31 117
MsaG005321 AT1G70060.2 64.706 85 30 0 2 86 5 89 3.03e-31 116
MsaG005321 AT1G70060.1 64.706 85 30 0 2 86 5 89 3.03e-31 116
MsaG005321 AT3G01320.2 65.854 82 28 0 4 85 50 131 1.93e-29 111
MsaG005321 AT3G01320.1 65.854 82 28 0 4 85 50 131 1.93e-29 111
MsaG005321 AT5G15020.2 64.935 77 27 0 3 79 44 120 5.33e-28 107
MsaG005321 AT5G15020.1 64.935 77 27 0 3 79 44 120 5.49e-28 107
MsaG005321 AT1G59890.3 62.162 74 28 0 12 85 46 119 1.40e-25 100
MsaG005321 AT1G59890.1 62.162 74 28 0 12 85 46 119 1.40e-25 100
MsaG005321 AT1G59890.2 62.162 74 28 0 12 85 46 119 1.46e-25 100
MsaG005321 AT1G59890.4 62.162 74 28 0 12 85 46 119 1.50e-25 100
MsaG005321 AT1G10450.2 64.706 68 24 0 12 79 34 101 4.48e-24 96.3
MsaG005321 AT1G10450.1 64.706 68 24 0 12 79 34 101 4.48e-24 96.3
MsaG005321 AT1G10450.3 64.706 68 24 0 12 79 85 152 4.62e-24 95.9
MsaG005321 AT1G24200.1 53.846 78 36 0 2 79 5 82 6.19e-24 91.3
MsaG005321 AT1G70030.3 47.312 93 47 1 1 93 12 102 1.98e-23 88.2
MsaG005321 AT1G70030.2 47.312 93 47 1 1 93 12 102 5.08e-23 87.4
MsaG005321 AT1G70030.1 47.312 93 47 1 1 93 1 91 1.20e-22 87.0
MsaG005321 AT1G27250.1 51.948 77 37 0 2 78 12 88 1.25e-22 86.3
MsaG005321 AT5G15025.1 54.217 83 35 1 7 89 43 122 2.01e-22 85.5
MsaG005321 AT1G27220.1 48.718 78 40 0 2 79 5 82 1.63e-21 84.7
MsaG005321 AT4G12020.4 51.190 84 40 1 2 85 299 381 2.26e-20 85.5
MsaG005321 AT4G12020.2 51.190 84 40 1 2 85 299 381 2.26e-20 85.5
MsaG005321 AT4G12020.11 51.190 84 40 1 2 85 299 381 2.57e-20 85.5
MsaG005321 AT4G12020.5 51.190 84 40 1 2 85 232 314 2.71e-20 85.5
MsaG005321 AT4G12020.3 51.190 84 40 1 2 85 299 381 2.72e-20 85.5
MsaG005321 AT4G12020.10 51.190 84 40 1 2 85 299 381 2.85e-20 85.1
MsaG005321 AT4G12020.1 51.190 84 40 1 2 85 299 381 2.96e-20 85.1
MsaG005321 AT4G12020.9 51.190 84 40 1 2 85 299 381 2.96e-20 85.1
MsaG005321 AT4G12020.7 51.190 84 40 1 2 85 299 381 2.99e-20 85.1
MsaG005321 AT4G12020.6 51.190 84 40 1 2 85 299 381 3.56e-20 85.1
MsaG005321 AT4G12020.8 51.190 84 40 1 2 85 299 381 3.56e-20 85.1
MsaG005321 AT5G15040.1 48.052 77 38 1 14 90 6 80 2.25e-19 76.6
MsaG005321 AT5G15040.3 48.052 77 38 1 14 90 6 80 2.25e-19 76.6
MsaG005321 AT5G15040.2 48.052 77 38 1 14 90 6 80 2.25e-19 76.6
MsaG005321 AT5G35610.1 46.988 83 44 0 3 85 2 84 2.47e-18 75.9
MsaG005321 AT1G24250.1 41.053 95 52 1 8 98 117 211 5.51e-17 74.3
MsaG005321 AT1G23810.1 38.947 95 54 1 8 98 106 200 3.51e-16 72.0
MsaG005321 AT1G24230.1 36.937 111 58 3 4 102 7 117 7.65e-15 68.6
MsaG005321 AT1G24230.1 35.526 76 49 0 14 89 89 164 8.44e-13 62.8
MsaG005321 AT1G27260.1 33.058 121 51 2 12 102 4 124 2.10e-13 64.3
MsaG005321 AT1G27260.1 41.538 65 38 0 8 72 90 154 2.34e-11 58.9
MsaG005321 AT1G24210.1 42.254 71 41 0 4 74 7 77 6.01e-13 62.0
MsaG005321 AT1G27280.1 41.791 67 39 0 8 74 78 144 6.17e-12 60.5

Find 14 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTACTGATTTCATATCCTTT+TGG 0.331804 1:+80168230 None:intergenic
GAGTGAAGGAGCTGTTTAAA+AGG 0.335263 1:-80168289 MsaT005321.1:CDS
AAATGTTATGAAAGAATTCA+AGG 0.402015 1:-80168345 MsaT005321.1:CDS
GAAAGTTATGAGGGATTTCA+AGG 0.411631 1:-80168165 MsaT005321.1:CDS
TAAGCACTTGACTACTGAAA+TGG 0.455251 1:-80168435 MsaT005321.1:CDS
TCTCGGAATTGAAAATGAAA+TGG 0.486789 1:-80168141 MsaT005321.1:CDS
TTCAACACCTTCCTGCCAAA+AGG 0.512524 1:-80168245 MsaT005321.1:CDS
ATGAGGGATTTCAAGGCTCT+CGG 0.525498 1:-80168158 MsaT005321.1:CDS
AAACGTTGATGATGATGAAA+GGG 0.527843 1:-80168207 MsaT005321.1:CDS
CAAACGTTGATGATGATGAA+AGG 0.538856 1:-80168208 MsaT005321.1:CDS
AGTCGAGTTTCAAGATGAGA+TGG 0.542797 1:-80168387 MsaT005321.1:CDS
TTATGAAAGAATTCAAGGCT+AGG 0.608401 1:-80168340 MsaT005321.1:CDS
TGGTGTCGTAGAAAGAGTGA+AGG 0.639497 1:-80168303 MsaT005321.1:CDS
GCTAGGAGAATTGATACAAG+TGG 0.644745 1:-80168323 MsaT005321.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 80168109 80168607 80168109 ID=MsaG005321
Chr1 mRNA 80168109 80168607 80168109 ID=MsaT005321.1;Parent=MsaG005321
Chr1 exon 80168109 80168465 80168109 ID=MsaT005321.1.exon2;Parent=MsaT005321.1
Chr1 CDS 80168109 80168465 80168109 ID=cds.MsaT005321.1;Parent=MsaT005321.1
Chr1 exon 80168605 80168607 80168605 ID=MsaT005321.1.exon1;Parent=MsaT005321.1
Chr1 CDS 80168605 80168607 80168605 ID=cds.MsaT005321.1;Parent=MsaT005321.1
Gene Sequence

>MsaT005321.1

ATGGGTTCGAGTAAGCACTTGACTACTGAAATGGCACTAGCATATCTGAAAGCAGTAAGAGTCGAGTTTCAAGATGAGATGGAAAAGTATGATGAATTTTTAAATGTTATGAAAGAATTCAAGGCTAGGAGAATTGATACAAGTGGTGTCGTAGAAAGAGTGAAGGAGCTGTTTAAAAGGCATAAAAATTTAATCTTGGGATTCAACACCTTCCTGCCAAAAGGATATGAAATCAGTACAAACGTTGATGATGATGAAAGGGAAAATTATGATGAATTTTTGAAAGTTATGAGGGATTTCAAGGCTCTCGGAATTGAAAATGAAATGGAGCAGATTGTTCAACTTTTGTCACGTCTATGA

Protein sequence

>MsaT005321.1

MGSSKHLTTEMALAYLKAVRVEFQDEMEKYDEFLNVMKEFKARRIDTSGVVERVKELFKRHKNLILGFNTFLPKGYEISTNVDDDERENYDEFLKVMRDFKALGIENEMEQIVQLLSRL*