AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005334

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005334 MtrunA17_Chr1g0207351 66.952 351 83 3 168 485 16 366 1.87e-159 456
MsaG005334 MtrunA17_Chr7g0252101 58.140 430 138 9 86 485 15 432 4.15e-157 453
MsaG005334 MtrunA17_Chr3g0093911 65.527 351 91 4 164 485 1 350 8.11e-152 436
MsaG005334 MtrunA17_Chr4g0075971 49.809 524 212 12 6 485 86 602 1.76e-146 432
MsaG005334 MtrunA17_Chr4g0075971 44.737 152 76 3 8 154 11 159 1.26e-27 116
MsaG005334 MtrunA17_Chr1g0209051 70.849 271 74 4 218 484 1 270 3.75e-128 373
MsaG005334 MtrunA17_Chr6g0488101 46.120 451 183 13 88 485 12 455 4.68e-112 338
MsaG005334 MtrunA17_Chr3g0077801 52.676 355 126 7 164 482 1 349 6.14e-107 322
MsaG005334 MtrunA17_Chr6g0488271 47.156 422 170 12 110 485 1 415 3.24e-106 322
MsaG005334 MtrunA17_Chr3g0077901 51.676 358 137 7 160 484 17 371 1.86e-104 316
MsaG005334 MtrunA17_Chr3g0077851 50.000 338 133 7 180 484 1 335 5.84e-92 283
MsaG005334 MtrunA17_Chr3g0112311 48.476 361 139 10 164 483 1 355 1.81e-91 282
MsaG005334 MtrunA17_Chr7g0243331 51.143 350 131 9 170 485 7 350 1.21e-90 280
MsaG005334 MtrunA17_Chr6g0452641 51.007 298 119 8 206 485 52 340 4.25e-79 250
MsaG005334 MtrunA17_Chr1g0202841 68.156 179 53 3 311 485 1 179 6.53e-78 241
MsaG005334 MtrunA17_Chr3g0123471 51.301 269 112 9 218 477 1 259 1.02e-67 218
MsaG005334 MtrunA17_Chr4g0075861 43.103 290 126 7 6 258 86 373 3.40e-58 196
MsaG005334 MtrunA17_Chr4g0075861 46.053 152 74 3 8 154 11 159 4.87e-30 120
MsaG005334 MtrunA17_Chr3g0122991 40.867 323 104 8 166 451 2 274 3.41e-58 193
MsaG005334 MtrunA17_Chr1g0187941 59.124 137 54 2 311 445 1 137 1.74e-43 150
MsaG005334 MtrunA17_Chr6g0486591 51.553 161 63 6 338 485 1 159 1.65e-37 135
MsaG005334 MtrunA17_Chr3g0077861 49.390 164 69 3 7 159 6 166 5.88e-36 131
MsaG005334 MtrunA17_Chr3g0077941 45.509 167 75 4 9 159 8 174 2.73e-35 129
MsaG005334 MtrunA17_Chr1g0196241 67.778 90 26 2 381 469 1 88 4.12e-35 126
MsaG005334 MtrunA17_Chr3g0077911 49.359 156 65 3 9 153 8 160 1.74e-34 127
MsaG005334 MtrunA17_Chr3g0077911 48.276 58 30 0 97 154 18 75 3.94e-11 61.6
MsaG005334 MtrunA17_Chr1g0209201 49.650 143 62 3 1 134 1 142 2.06e-34 126
MsaG005334 MtrunA17_Chr1g0209201 56.250 64 27 1 88 151 20 82 2.04e-15 73.2
MsaG005334 MtrunA17_Chr3g0077891 45.238 168 75 4 9 159 8 175 3.39e-34 126
MsaG005334 MtrunA17_Chr1g0196231 53.793 145 40 4 161 280 224 366 3.07e-33 129
MsaG005334 MtrunA17_Chr1g0206771 44.898 147 77 2 10 152 17 163 8.01e-33 123
MsaG005334 MtrunA17_Chr1g0206771 50.685 73 36 0 5 77 91 163 9.79e-17 78.2
MsaG005334 MtrunA17_Chr1g0206771 52.308 65 31 0 83 147 15 79 9.01e-15 72.4
MsaG005334 MtrunA17_Chr1g0209191 59.000 100 32 2 392 484 1 98 1.27e-31 117
MsaG005334 MtrunA17_Chr3g0077881 43.452 168 78 4 9 159 8 175 1.23e-30 117
MsaG005334 MtrunA17_Chr1g0205821 42.675 157 79 2 8 153 12 168 1.12e-29 114
MsaG005334 MtrunA17_Chr1g0205821 42.529 87 50 0 85 171 14 100 6.45e-16 75.9
MsaG005334 MtrunA17_Chr1g0205821 45.333 75 41 0 4 78 94 168 1.93e-13 68.6
MsaG005334 MtrunA17_Chr7g0243391 38.426 216 99 6 160 345 14 225 1.18e-29 116
MsaG005334 MtrunA17_Chr1g0209061 51.852 108 30 2 87 194 13 98 6.09e-26 101
MsaG005334 MtrunA17_Chr8g0364231 40.667 150 78 1 8 146 41 190 1.11e-25 111
MsaG005334 MtrunA17_Chr8g0364231 60.000 70 28 0 85 154 43 112 2.72e-19 91.7
MsaG005334 MtrunA17_Chr1g0205831 38.776 147 82 2 11 155 4 144 1.83e-24 100
MsaG005334 MtrunA17_Chr1g0205831 39.423 104 59 2 87 186 5 108 3.07e-11 62.4
MsaG005334 MtrunA17_Chr7g0218891 39.130 161 85 4 9 156 99 259 7.22e-23 98.2
MsaG005334 MtrunA17_Chr7g0218891 37.975 158 85 4 10 154 14 171 3.73e-20 90.5
MsaG005334 MtrunA17_Chr7g0218891 48.649 74 36 2 83 154 12 85 6.68e-12 65.9
MsaG005334 MtrunA17_Chr5g0415031 37.748 151 83 1 8 147 42 192 2.18e-22 101
MsaG005334 MtrunA17_Chr5g0415031 53.571 84 38 1 85 168 44 126 2.05e-18 89.0
MsaG005334 MtrunA17_Chr6g0452001 38.000 150 82 1 8 146 62 211 2.31e-22 101
MsaG005334 MtrunA17_Chr6g0452001 57.971 69 29 0 85 153 64 132 1.52e-17 86.3
MsaG005334 MtrunA17_Chr1g0206781 45.536 112 46 1 10 121 12 108 9.07e-22 90.5
MsaG005334 MtrunA17_Chr1g0206781 54.286 70 32 0 83 152 10 79 6.50e-18 79.7
MsaG005334 MtrunA17_Chr6g0452021 35.374 147 86 1 9 146 43 189 1.25e-20 94.4
MsaG005334 MtrunA17_Chr6g0452021 48.148 81 42 0 85 165 44 124 5.51e-16 80.1
MsaG005334 MtrunA17_Chr1g0206951 41.593 113 39 1 167 252 2 114 1.48e-18 82.0
MsaG005334 MtrunA17_Chr2g0328701 45.545 101 47 2 8 103 28 125 1.85e-18 81.6
MsaG005334 MtrunA17_Chr6g0459061 29.825 228 149 7 226 449 98 318 2.50e-18 86.7
MsaG005334 MtrunA17_Chr1g0205811 42.105 95 52 1 85 176 15 109 3.22e-17 77.8
MsaG005334 MtrunA17_Chr1g0205811 47.887 71 37 0 9 79 14 84 2.53e-14 69.7
MsaG005334 MtrunA17_Chr7g0218911 46.269 67 36 0 88 154 2 68 2.54e-15 72.4
MsaG005334 MtrunA17_Chr1g0205801 47.887 71 37 0 85 155 13 83 4.28e-15 71.6
MsaG005334 MtrunA17_Chr1g0205861 45.588 68 37 0 85 152 17 84 1.08e-14 70.5
MsaG005334 MtrunA17_Chr1g0205861 44.286 70 39 0 8 77 15 84 1.52e-12 64.3
MsaG005334 MtrunA17_Chr3g0077811 47.222 72 37 1 9 79 13 84 4.56e-14 68.9
MsaG005334 MtrunA17_Chr3g0077811 53.704 54 25 0 102 155 32 85 1.85e-13 67.0
MsaG005334 MtrunA17_Chr5g0431721 48.684 76 31 3 8 81 10 79 1.31e-12 64.3
MsaG005334 MtrunA17_Chr8g0364021 35.294 102 61 2 85 184 45 143 3.79e-12 66.2
MsaG005334 MtrunA17_Chr8g0364021 45.833 72 39 0 7 78 42 113 8.25e-12 65.1
MsaG005334 MtrunA17_Chr7g0256011 45.714 70 38 0 83 152 15 84 1.19e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005334 AT5G15020.2 42.568 148 76 2 8 146 51 198 2.01e-24 107
MsaG005334 AT5G15020.2 59.420 69 28 0 85 153 53 121 1.40e-17 86.7
MsaG005334 AT5G15020.1 42.568 148 76 2 8 146 51 198 2.03e-24 107
MsaG005334 AT5G15020.1 59.420 69 28 0 85 153 53 121 1.40e-17 86.7
MsaG005334 AT1G24190.2 39.333 150 80 2 8 146 13 162 1.29e-23 105
MsaG005334 AT1G24190.2 42.202 109 44 1 85 193 15 104 1.60e-17 86.7
MsaG005334 AT1G24190.1 39.333 150 80 2 8 146 13 162 1.36e-23 105
MsaG005334 AT1G24190.1 42.202 109 44 1 85 193 15 104 1.54e-17 86.7
MsaG005334 AT1G24190.3 39.333 150 80 2 8 146 13 162 1.37e-23 105
MsaG005334 AT1G24190.3 42.202 109 44 1 85 193 15 104 1.55e-17 86.7
MsaG005334 AT1G24230.1 31.677 161 105 2 8 167 13 169 6.96e-23 97.8
MsaG005334 AT1G70060.2 38.411 151 81 1 8 146 13 163 1.62e-22 102
MsaG005334 AT1G70060.2 51.190 84 41 0 85 168 15 98 1.97e-16 83.2
MsaG005334 AT1G70060.1 38.411 151 81 1 8 146 13 163 1.62e-22 102
MsaG005334 AT1G70060.1 51.190 84 41 0 85 168 15 98 1.97e-16 83.2
MsaG005334 AT1G10450.3 38.776 147 81 2 9 146 84 230 2.77e-22 101
MsaG005334 AT1G10450.3 53.623 69 32 0 85 153 85 153 5.09e-15 78.6
MsaG005334 AT1G10450.2 38.776 147 81 2 9 146 33 179 3.05e-22 101
MsaG005334 AT1G10450.2 53.623 69 32 0 85 153 34 102 5.52e-15 78.6
MsaG005334 AT1G10450.1 38.776 147 81 2 9 146 33 179 3.05e-22 101
MsaG005334 AT1G10450.1 53.623 69 32 0 85 153 34 102 5.52e-15 78.6
MsaG005334 AT3G01320.2 36.913 149 84 2 8 146 56 204 1.21e-21 99.4
MsaG005334 AT3G01320.2 59.420 69 28 0 85 153 58 126 1.98e-18 89.4
MsaG005334 AT3G01320.1 36.913 149 84 2 8 146 56 204 1.23e-21 99.4
MsaG005334 AT3G01320.1 59.420 69 28 0 85 153 58 126 1.92e-18 89.4
MsaG005334 AT1G59890.4 37.415 147 83 1 9 146 45 191 2.25e-21 98.6
MsaG005334 AT1G59890.4 45.714 105 52 1 85 189 46 145 4.05e-18 88.2
MsaG005334 AT1G59890.2 37.415 147 83 1 9 146 45 191 2.45e-21 98.2
MsaG005334 AT1G59890.2 45.714 105 52 1 85 189 46 145 3.82e-18 88.6
MsaG005334 AT1G59890.3 37.415 147 83 1 9 146 45 191 2.49e-21 98.2
MsaG005334 AT1G59890.3 45.714 105 52 1 85 189 46 145 3.91e-18 88.6
MsaG005334 AT1G59890.1 37.415 147 83 1 9 146 45 191 2.53e-21 98.2
MsaG005334 AT1G59890.1 45.714 105 52 1 85 189 46 145 4.03e-18 88.2
MsaG005334 AT1G24200.1 33.333 147 90 1 8 146 13 159 3.73e-19 85.9
MsaG005334 AT1G24200.1 48.529 68 35 0 85 152 15 82 3.75e-13 68.6
MsaG005334 AT1G70030.1 34.459 148 86 2 9 146 11 157 2.23e-18 82.8
MsaG005334 AT1G70030.1 36.449 107 64 1 85 191 12 114 6.41e-14 70.1
MsaG005334 AT1G27260.1 30.481 187 108 3 10 181 4 183 6.45e-18 83.2
MsaG005334 AT5G15025.1 53.623 69 32 0 85 153 48 116 4.04e-17 77.8
MsaG005334 AT5G15025.1 47.887 71 37 0 8 78 46 116 5.44e-13 66.2
MsaG005334 AT1G23810.1 28.351 194 112 4 8 181 13 199 4.20e-16 78.2
MsaG005334 AT1G23810.1 30.147 136 82 2 8 142 108 231 4.52e-13 69.3
MsaG005334 AT1G70030.3 50.000 66 33 0 88 153 26 91 2.48e-14 70.1
MsaG005334 AT1G70030.3 44.286 70 39 0 9 78 22 91 2.14e-11 61.6
MsaG005334 AT1G70030.2 49.275 69 35 0 85 153 23 91 4.64e-14 69.7
MsaG005334 AT1G24250.1 30.935 139 83 2 8 145 119 245 8.26e-14 71.6
MsaG005334 AT1G27250.1 45.070 71 39 0 2 72 14 84 6.33e-13 66.2
MsaG005334 AT1G27250.1 47.619 63 33 0 85 147 22 84 2.55e-12 64.7
MsaG005334 AT4G12020.11 44.944 89 48 1 9 97 308 395 8.99e-13 71.6
MsaG005334 AT4G12020.11 49.275 69 34 1 85 153 309 376 1.01e-12 71.2
MsaG005334 AT4G12020.7 44.944 89 48 1 9 97 308 395 9.38e-13 71.6
MsaG005334 AT4G12020.7 49.275 69 34 1 85 153 309 376 9.71e-13 71.6
MsaG005334 AT4G12020.1 44.944 89 48 1 9 97 308 395 9.38e-13 71.6
MsaG005334 AT4G12020.1 49.275 69 34 1 85 153 309 376 1.02e-12 71.2
MsaG005334 AT4G12020.9 44.944 89 48 1 9 97 308 395 9.38e-13 71.6
MsaG005334 AT4G12020.9 49.275 69 34 1 85 153 309 376 1.02e-12 71.2
MsaG005334 AT4G12020.10 49.275 69 34 1 85 153 309 376 9.45e-13 71.6
MsaG005334 AT4G12020.10 44.944 89 48 1 9 97 308 395 9.87e-13 71.6
MsaG005334 AT4G12020.3 44.944 89 48 1 9 97 308 395 9.45e-13 71.6
MsaG005334 AT4G12020.3 49.275 69 34 1 85 153 309 376 9.70e-13 71.6
MsaG005334 AT4G12020.5 49.275 69 34 1 85 153 242 309 9.48e-13 71.6
MsaG005334 AT4G12020.5 44.944 89 48 1 9 97 241 328 9.65e-13 71.6
MsaG005334 AT4G12020.6 49.275 69 34 1 85 153 309 376 9.69e-13 71.6
MsaG005334 AT4G12020.6 44.944 89 48 1 9 97 308 395 9.86e-13 71.6
MsaG005334 AT4G12020.8 49.275 69 34 1 85 153 309 376 9.69e-13 71.6
MsaG005334 AT4G12020.8 44.944 89 48 1 9 97 308 395 9.86e-13 71.6
MsaG005334 AT4G12020.4 44.944 89 48 1 9 97 308 395 9.88e-13 71.6
MsaG005334 AT4G12020.4 49.275 69 34 1 85 153 309 376 9.97e-13 71.6
MsaG005334 AT4G12020.2 44.944 89 48 1 9 97 308 395 9.89e-13 71.6
MsaG005334 AT4G12020.2 49.275 69 34 1 85 153 309 376 1.01e-12 71.6
MsaG005334 AT1G27270.1 29.000 200 111 4 6 181 11 203 3.50e-11 63.9
MsaG005334 AT1G27220.1 42.857 63 36 0 85 147 15 77 4.35e-11 62.4
MsaG005334 AT1G27220.1 37.778 90 56 0 8 97 13 102 5.85e-11 62.0
MsaG005334 AT5G15040.1 39.706 68 41 0 86 153 5 72 8.03e-11 58.9
MsaG005334 AT5G15040.3 39.706 68 41 0 86 153 5 72 8.03e-11 58.9
MsaG005334 AT5G15040.2 39.706 68 41 0 86 153 5 72 8.03e-11 58.9

Find 82 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTCTTTATTCTTGTAAA+TGG 0.059967 1:+80314284 None:intergenic
GACATTGCATTATTTGTTTC+TGG 0.167467 1:-80313828 MsaT005334.1:CDS
CTCTCATATCCCCACATGTT+TGG 0.231037 1:+80313771 None:intergenic
CTTCTTCAAGTTTCGTATCT+TGG 0.270913 1:-80314458 MsaT005334.1:intron
AGTAACCCAACTACAATTTC+GGG 0.277382 1:+80314133 None:intergenic
GAGTAACCCAACTACAATTT+CGG 0.302395 1:+80314132 None:intergenic
GACCATGCCTTGCTTGCTTT+TGG 0.305642 1:-80314218 MsaT005334.1:CDS
GCAAAATGTAATGTGTATTC+CGG 0.315316 1:-80313717 MsaT005334.1:CDS
AACCTAGGGCACATGGAAAA+TGG 0.324840 1:+80313960 None:intergenic
TTCCAATTCGACATAATTCA+TGG 0.327766 1:+80313860 None:intergenic
TCTCATATCCCCACATGTTT+GGG 0.328849 1:+80313772 None:intergenic
AACCATGAATTATGTCGAAT+TGG 0.334643 1:-80313862 MsaT005334.1:CDS
CTCTGAGGAATTTGTCTTGG+TGG 0.339559 1:-80314189 MsaT005334.1:CDS
GAGCTGAATTTCAATATAAC+AGG 0.361061 1:-80316947 MsaT005334.1:CDS
AGAATGAAGAAGCTGCTTAA+AGG 0.383126 1:-80316624 MsaT005334.1:CDS
AAAAGTGATGCACGAGGACT+TGG 0.383593 1:+80313660 None:intergenic
CCAGTGGTCTTAAGTTGAAA+TGG 0.385161 1:+80316540 None:intergenic
CCCCTAGCTCTGAATTTAAC+TGG 0.386314 1:-80314253 MsaT005334.1:CDS
CAAAATGTAATGTGTATTCC+GGG 0.391174 1:-80313716 MsaT005334.1:CDS
CAAAGTTATGAGGGATTTCA+AGG 0.391778 1:-80316679 MsaT005334.1:CDS
AGAGTGAAGAGGTTGTTTAA+TGG 0.392841 1:-80316849 MsaT005334.1:CDS
AAACTGCGTTTCAAGATAAC+AGG 0.393075 1:-80316722 MsaT005334.1:CDS
GTCGTCGAAATCTAGTCTTT+TGG 0.398478 1:+80314568 None:intergenic
CCTTCTTTATTCTTGTAAAT+GGG 0.403862 1:+80314285 None:intergenic
TTGGTCGGGTATGCTAATAA+TGG 0.412378 1:+80314421 None:intergenic
ACCAATCCCGAAATTGTAGT+TGG 0.426395 1:-80314139 MsaT005334.1:CDS
AAGACAACTGTGAAGTTCCT+TGG 0.435557 1:-80314610 MsaT005334.1:CDS
CATCAAATCACACTTCCATC+AGG 0.438813 1:-80316780 MsaT005334.1:CDS
ACCAGTTAAATTCAGAGCTA+GGG 0.450231 1:+80314252 None:intergenic
TATGTCGAATTGGAAACTTT+AGG 0.451341 1:-80313852 MsaT005334.1:CDS
CCCAACTACAATTTCGGGAT+TGG 0.452080 1:+80314138 None:intergenic
AATGTATAGATGAAGATCTC+TGG 0.452496 1:-80317008 None:intergenic
TTCCATCTCTGAAATCTAGT+AGG 0.457834 1:+80316898 None:intergenic
AATTTATTGTTTGAAGGTGA+TGG 0.459898 1:-80314396 MsaT005334.1:CDS
CACATGGAAAATGGTACATC+AGG 0.463406 1:+80313969 None:intergenic
AGTGTGATTTGATGTTCCTT+TGG 0.466154 1:+80316789 None:intergenic
TGGGTTACTCATTCGACAAT+GGG 0.466484 1:-80314119 MsaT005334.1:CDS
GACCAGTTAAATTCAGAGCT+AGG 0.479297 1:+80314251 None:intergenic
AGTGTGATTCGATGCTGTTT+TGG 0.491674 1:+80316564 None:intergenic
AGACAACTGTGAAGTTCCTT+GGG 0.493013 1:-80314609 MsaT005334.1:CDS
TCAAGTTTCGTATCTTGGTA+AGG 0.495617 1:-80314453 MsaT005334.1:intron
CCATTTCAACTTAAGACCAC+TGG 0.499321 1:-80316540 MsaT005334.1:intron
CATTCGACAATGGGAGATCA+AGG 0.501174 1:-80314110 MsaT005334.1:CDS
CCCATTTACAAGAATAAAGA+AGG 0.503369 1:-80314285 MsaT005334.1:CDS
TTGATGTTCCTTTGGTAGGA+AGG 0.503740 1:+80316797 None:intergenic
CCAATCCCGAAATTGTAGTT+GGG 0.507102 1:-80314138 MsaT005334.1:CDS
ATGCTCTGAGGAATTTGTCT+TGG 0.509803 1:-80314192 MsaT005334.1:CDS
CTCCTACTAGATTTCAGAGA+TGG 0.525821 1:-80316900 MsaT005334.1:CDS
TGGAAACCGCGATGATGCAT+TGG 0.530913 1:-80316988 MsaT005334.1:CDS
ACATGAAGTAGATTATTCTC+TGG 0.541113 1:-80313604 MsaT005334.1:CDS
TCACTAACAAGGCCAACATA+GGG 0.546011 1:-80314043 MsaT005334.1:CDS
TGATTTGATGTTCCTTTGGT+AGG 0.550872 1:+80316793 None:intergenic
GTCACTAACAAGGCCAACAT+AGG 0.554169 1:-80314044 MsaT005334.1:CDS
TTATCACAATTAACCAACCT+AGG 0.555400 1:+80313945 None:intergenic
TTGGGTTACTCATTCGACAA+TGG 0.566980 1:-80314120 MsaT005334.1:CDS
CAAGCTGAGTACCCCTATGT+TGG 0.568839 1:+80314031 None:intergenic
TTTGCAACTTTAAACCCTGA+TGG 0.569059 1:+80316765 None:intergenic
GCATTTGCCAAAAGCAAGCA+AGG 0.573799 1:+80314211 None:intergenic
ATTAACCAACCTAGGGCACA+TGG 0.579393 1:+80313953 None:intergenic
TGCCAAAAGCAAGCAAGGCA+TGG 0.592287 1:+80314216 None:intergenic
TTGTCAAAGCAAGAGTGAAG+AGG 0.596584 1:-80316860 MsaT005334.1:CDS
ATCAAATCACACTTCCATCA+GGG 0.599894 1:-80316779 MsaT005334.1:CDS
TATCACAATTAACCAACCTA+GGG 0.600311 1:+80313946 None:intergenic
TCTGCTAGAAGACGTAACAT+AGG 0.607130 1:+80314355 None:intergenic
ATTATATCAAGCACATCCCA+AGG 0.612864 1:+80314593 None:intergenic
CATTGAGCAACCCAAACATG+TGG 0.616398 1:-80313782 MsaT005334.1:CDS
CGACAATGGGAGATCAAGGG+AGG 0.621376 1:-80314106 MsaT005334.1:CDS
ACACACCGGAAAATTGAAAG+AGG 0.631839 1:+80314546 None:intergenic
GGTAGACATGCAAATGACGT+TGG 0.632137 1:+80314159 None:intergenic
AATTGATACACAATATGTCG+TGG 0.632933 1:-80316649 MsaT005334.1:CDS
CCAGTTAAATTCAGAGCTAG+GGG 0.632990 1:+80314253 None:intergenic
ATTGAGCAACCCAAACATGT+GGG 0.632993 1:-80313781 MsaT005334.1:CDS
ATTCAACACCTTCCTACCAA+AGG 0.639776 1:-80316805 MsaT005334.1:CDS
CACTAACAAGGCCAACATAG+GGG 0.650581 1:-80314042 MsaT005334.1:CDS
AATATATGTTGTCACTAACA+AGG 0.651381 1:-80314054 MsaT005334.1:CDS
ATTCGACAATGGGAGATCAA+GGG 0.657745 1:-80314109 MsaT005334.1:CDS
GACAATGGGAGATCAAGGGA+GGG 0.660902 1:-80314105 MsaT005334.1:CDS
TTGAGCAACCCAAACATGTG+GGG 0.672229 1:-80313780 MsaT005334.1:CDS
GTGGGGATATGAGAGCAACT+CGG 0.682589 1:-80313763 MsaT005334.1:CDS
GATACGCCAATGCATCATCG+CGG 0.757377 1:+80316982 None:intergenic
AAAATGTAATGTGTATTCCG+GGG 0.783176 1:-80313715 MsaT005334.1:CDS
ATGATGAAAAGTGATGCACG+AGG 0.803439 1:+80313654 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 80313596 80317021 80313596 ID=MsaG005334
Chr1 mRNA 80313596 80317021 80313596 ID=MsaT005334.1;Parent=MsaG005334
Chr1 exon 80313596 80314470 80313596 ID=MsaT005334.1.exon3;Parent=MsaT005334.1
Chr1 CDS 80313596 80314470 80313596 ID=cds.MsaT005334.1;Parent=MsaT005334.1
Chr1 exon 80314552 80314653 80314552 ID=MsaT005334.1.exon2;Parent=MsaT005334.1
Chr1 CDS 80314552 80314653 80314552 ID=cds.MsaT005334.1;Parent=MsaT005334.1
Chr1 exon 80316541 80317021 80316541 ID=MsaT005334.1.exon1;Parent=MsaT005334.1
Chr1 CDS 80316541 80317021 80316541 ID=cds.MsaT005334.1;Parent=MsaT005334.1
Gene Sequence

>MsaT005334.1

ATGAAGATCTCTGGAAACCGCGATGATGCATTGGCGTATCTTAAAGCAGTAAGAGCTGAATTTCAATATAACAGGATAAAGTATGATGAGTTGTTAAAACTCCTACTAGATTTCAGAGATGGAAAAATTGATATAAGAGTTGTCAAAGCAAGAGTGAAGAGGTTGTTTAATGGCCATGCAGATTTAATTTTTGGATTCAACACCTTCCTACCAAAGGAACATCAAATCACACTTCCATCAGGGTTTAAAGTTGCAAAAACATTTCTTAAGGAAGTAGAAACTGCGTTTCAAGATAACAGGAAAAAGTATGATCAGTTTTTCAAAGTTATGAGGGATTTCAAGGATCAAAGAATTGATACACAATATGTCGTGGCAAGAATGAAGAAGCTGCTTAAAGGACATTCAGATTTAATTTTGAGATTCAACACCTTCCTGCCAAAACAGCATCGAATCACACTTCCATTTCAACTTAAGACCACTGGAAAAAAGGTGAAGAAGGTAGAAGACAACTGTGAAGTTCCTTGGGATGTGCTTGATATAATTTCCAAAAGACTAGATTTCGACGACCTCTTTCAATTTTCCGTTTCGTATCTTGGTAAGGAATCATTTTCCATTATTAGCATACCCGACCAAAAAATTTATTGTTTGAAGGTGATGGAGCACGTCTTCGACTATACCTATGTTACGTCTTCTAGCAGATATTTTATCACAACAGAGGACAATAATAATTCATTTTTGCTAATCAACCCATTTACAAGAATAAAGAAGGTAATCAGTACCCCTAGCTCTGAATTTAACTGGTCTCTTTTAATTGACCATGCCTTGCTTGCTTTTGGCAAATGCTCTGAGGAATTTGTCTTGGTGGTTTTATGCCAACGTCATTTGCATGTCTACCAATCCCGAAATTGTAGTTGGGTTACTCATTCGACAATGGGAGATCAAGGGAGGGTTGTTGACTTTGTCGTTCTACATAATGTAATATATGTTGTCACTAACAAGGCCAACATAGGGGTACTCAGCTTGAATTCTGCAAATATTAGATTTCTAAAACTAAAGAGTACTCCTGATGTACCATTTTCCATGTGCCCTAGGTTGGTTAATTGTGATAAACAACTTTTAGTGGTTTTAATGGTCGATGAAATAAGGAATGTGTACAAGATAGACTTTTCAACCATGAATTATGTCGAATTGGAAACTTTAGGCGACATTGCATTATTTGTTTCTGGTGTTTTGAAAAGAAACTGCTATGCATTGAGCAACCCAAACATGTGGGGATATGAGAGCAACTCGGTGTATGTCATCAGAATTTTATCTGCAAAATGTAATGTGTATTCCGGGGATGATAGAAAATTGCAAAAATGCATAGCGCTTCCAAGTCCTCGTGCATCACTTTTCATCATGTTCGATTGGTGTTTTAGACATCTACAACATGAAGTAGATTATTCTCTGGTTGAGTAA
Protein sequence

>MsaT005334.1

MKISGNRDDALAYLKAVRAEFQYNRIKYDELLKLLLDFRDGKIDIRVVKARVKRLFNGHADLIFGFNTFLPKEHQITLPSGFKVAKTFLKEVETAFQDNRKKYDQFFKVMRDFKDQRIDTQYVVARMKKLLKGHSDLILRFNTFLPKQHRITLPFQLKTTGKKVKKVEDNCEVPWDVLDIISKRLDFDDLFQFSVSYLGKESFSIISIPDQKIYCLKVMEHVFDYTYVTSSSRYFITTEDNNNSFLLINPFTRIKKVISTPSSEFNWSLLIDHALLAFGKCSEEFVLVVLCQRHLHVYQSRNCSWVTHSTMGDQGRVVDFVVLHNVIYVVTNKANIGVLSLNSANIRFLKLKSTPDVPFSMCPRLVNCDKQLLVVLMVDEIRNVYKIDFSTMNYVELETLGDIALFVSGVLKRNCYALSNPNMWGYESNSVYVIRILSAKCNVYSGDDRKLQKCIALPSPRASLFIMFDWCFRHLQHEVDYSLVE*