Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG005365 | XP_003592478.1 | 87.678 | 211 | 9 | 3 | 1 | 211 | 1 | 194 | 9.36e-121 | 350 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG005365 | sp|Q9SN23|LBD38_ARATH | 68.644 | 118 | 35 | 1 | 1 | 118 | 1 | 116 | 2.06e-53 | 174 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG005365 | tr|G7IE59|G7IE59_MEDTR | 87.678 | 211 | 9 | 3 | 1 | 211 | 1 | 194 | 4.47e-121 | 350 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG005365 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG005365 | MtrunA17_Chr1g0207471 | 87.678 | 211 | 9 | 3 | 1 | 211 | 1 | 194 | 8.61e-125 | 350 |
| MsaG005365 | MtrunA17_Chr5g0401401 | 65.873 | 126 | 40 | 2 | 1 | 126 | 1 | 123 | 3.20e-55 | 175 |
| MsaG005365 | MtrunA17_Chr4g0053911 | 70.085 | 117 | 33 | 1 | 1 | 117 | 1 | 115 | 1.12e-54 | 174 |
| MsaG005365 | MtrunA17_Chr7g0215281 | 61.538 | 104 | 40 | 0 | 1 | 104 | 3 | 106 | 1.67e-46 | 155 |
| MsaG005365 | MtrunA17_Chr6g0457431 | 60.577 | 104 | 41 | 0 | 1 | 104 | 3 | 106 | 2.44e-45 | 148 |
| MsaG005365 | MtrunA17_Chr6g0469271 | 52.727 | 110 | 42 | 2 | 1 | 104 | 3 | 108 | 8.46e-38 | 130 |
| MsaG005365 | MtrunA17_Chr6g0470651 | 49.057 | 106 | 52 | 1 | 1 | 104 | 3 | 108 | 7.24e-35 | 123 |
| MsaG005365 | MtrunA17_Chr6g0470631 | 41.732 | 127 | 67 | 2 | 1 | 125 | 3 | 124 | 2.25e-33 | 119 |
| MsaG005365 | MtrunA17_Chr1g0209841 | 36.875 | 160 | 86 | 5 | 1 | 157 | 3 | 150 | 1.95e-30 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG005365 | AT3G49940.1 | 68.644 | 118 | 35 | 1 | 1 | 118 | 1 | 116 | 2.10e-54 | 174 |
| MsaG005365 | AT5G67420.2 | 37.698 | 252 | 114 | 4 | 1 | 211 | 1 | 250 | 6.84e-52 | 168 |
| MsaG005365 | AT5G67420.1 | 37.698 | 252 | 114 | 4 | 1 | 211 | 1 | 250 | 6.84e-52 | 168 |
| MsaG005365 | AT4G37540.1 | 60.741 | 135 | 44 | 3 | 1 | 129 | 1 | 132 | 1.28e-50 | 164 |
| MsaG005365 | AT3G02550.1 | 61.538 | 104 | 40 | 0 | 1 | 104 | 3 | 106 | 1.21e-46 | 155 |
| MsaG005365 | AT1G67100.1 | 60.000 | 105 | 42 | 0 | 1 | 105 | 3 | 107 | 4.93e-44 | 147 |
| MsaG005365 | AT1G68510.1 | 48.462 | 130 | 65 | 1 | 1 | 130 | 3 | 130 | 1.04e-41 | 141 |
Find 53 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAACAGCAAGAGTTTATTT+TGG | 0.186602 | 1:+80640768 | MsaT005365.1:CDS |
| AGCCCAACTGCTCCATTTAC+TGG | 0.215944 | 1:-80640607 | None:intergenic |
| TTTATGACATACATGATCTT+TGG | 0.296751 | 1:-80640373 | None:intergenic |
| AGAACTATAAATCCAGTAAA+TGG | 0.325401 | 1:+80640595 | MsaT005365.1:CDS |
| ATGGAGCAGTTGGGCTGTTA+TGG | 0.333353 | 1:+80640614 | MsaT005365.1:CDS |
| GTCTTTGTCACCAAGTTCTT+CGG | 0.371969 | 1:+80640308 | MsaT005365.1:CDS |
| TGTAATGAAGAAGCACAATC+TGG | 0.379276 | 1:+80640799 | MsaT005365.1:CDS |
| GATCTTTGGTTGGGAGGAAC+AGG | 0.395798 | 1:-80640359 | None:intergenic |
| GACATACATGATCTTTGGTT+GGG | 0.427328 | 1:-80640368 | None:intergenic |
| CACTTATGTATGAAGCTGTT+GGG | 0.452221 | 1:+80640572 | MsaT005365.1:CDS |
| TGGCGTTAGCCTGTGCTTGT+GGG | 0.452316 | 1:-80640283 | None:intergenic |
| GACACATGAGTCTGAGGAAT+CGG | 0.458754 | 1:+80640888 | MsaT005365.1:CDS |
| TCCACACCTAACAACTGATC+AGG | 0.464754 | 1:-80640712 | None:intergenic |
| GGTGGCACGGCCGAAGAACT+TGG | 0.467396 | 1:-80640318 | None:intergenic |
| AATCCAGTAAATGGAGCAGT+TGG | 0.467406 | 1:+80640604 | MsaT005365.1:CDS |
| GAATCGGAGTCGTCCACGTT+GGG | 0.467550 | 1:+80640904 | MsaT005365.1:CDS |
| GCACTCCCTGATCAGTTGTT+AGG | 0.477941 | 1:+80640706 | MsaT005365.1:CDS |
| GTGGCGTTAGCCTGTGCTTG+TGG | 0.478831 | 1:-80640284 | None:intergenic |
| TGACATACATGATCTTTGGT+TGG | 0.489850 | 1:-80640369 | None:intergenic |
| CCCTGATCAGTTGTTAGGTG+TGG | 0.497837 | 1:+80640711 | MsaT005365.1:CDS |
| CCAAAATAAACTCTTGCTGT+TGG | 0.526318 | 1:-80640768 | None:intergenic |
| ATCCAGTAAATGGAGCAGTT+GGG | 0.536591 | 1:+80640605 | MsaT005365.1:CDS |
| TCACTTATGTATGAAGCTGT+TGG | 0.539852 | 1:+80640571 | MsaT005365.1:CDS |
| TGCATCCCTTTCTCAAAACT+CGG | 0.542491 | 1:-80640217 | None:intergenic |
| GAACAAGTGCTGAGAGGTAA+CGG | 0.548928 | 1:+80640670 | MsaT005365.1:CDS |
| GAGATCATAAGAATAGTGTA+AGG | 0.555999 | 1:-80640993 | None:intergenic |
| GATACAAAACCCACAAGCAC+AGG | 0.556238 | 1:+80640274 | MsaT005365.1:CDS |
| TATAATAAAATGAGTTGCAA+CGG | 0.559441 | 1:+80640191 | None:intergenic |
| TTTGAGAAAGGGATGCAGTG+AGG | 0.560200 | 1:+80640223 | MsaT005365.1:CDS |
| ATAAGAATAGTGTAAGGAGT+TGG | 0.568732 | 1:-80640987 | None:intergenic |
| CAGCTTCATACATAAGTGAC+TGG | 0.569517 | 1:-80640567 | None:intergenic |
| GTAATGAAGAAGCACAATCT+GGG | 0.578649 | 1:+80640800 | MsaT005365.1:CDS |
| ATGCTACTTCTCAAACTCAA+AGG | 0.580159 | 1:+80640962 | MsaT005365.1:CDS |
| ATACATGATCTTTGGTTGGG+AGG | 0.585857 | 1:-80640365 | None:intergenic |
| AGTACTCTAGAGTGGTGAGA+AGG | 0.591970 | 1:+80640833 | MsaT005365.1:CDS |
| GGCTGTTATGGACTGGAAAG+TGG | 0.603769 | 1:+80640626 | MsaT005365.1:CDS |
| GAAAAGACATGAGGGTGGCA+CGG | 0.613200 | 1:-80640331 | None:intergenic |
| GCAGTTGGGCTGTTATGGAC+TGG | 0.615948 | 1:+80640619 | MsaT005365.1:CDS |
| CCACACCTAACAACTGATCA+GGG | 0.634962 | 1:-80640711 | None:intergenic |
| GATTTCACAGTACTCTAGAG+TGG | 0.637833 | 1:+80640825 | MsaT005365.1:CDS |
| TGCGGAGACACATGAGTCTG+AGG | 0.638167 | 1:+80640882 | MsaT005365.1:CDS |
| GGAATCGGAGTCGTCCACGT+TGG | 0.646426 | 1:+80640903 | MsaT005365.1:CDS |
| AAGTGGCAATTCTGTCAACT+TGG | 0.652925 | 1:+80640643 | MsaT005365.1:CDS |
| CCAAGCAGAAAAGCAACGTG+CGG | 0.655633 | 1:+80640864 | MsaT005365.1:CDS |
| TTCTGTTCTACTTCCCAACG+TGG | 0.659625 | 1:-80640917 | None:intergenic |
| CAGGTGAAAGAAAAGACATG+AGG | 0.660626 | 1:-80640340 | None:intergenic |
| AGGTGAAAGAAAAGACATGA+GGG | 0.666800 | 1:-80640339 | None:intergenic |
| TAACAACTGATCAGGGAGTG+CGG | 0.677605 | 1:-80640704 | None:intergenic |
| TGCTACGAGATTGTCTAACG+TGG | 0.685370 | 1:+80640252 | MsaT005365.1:CDS |
| GAACTTGGTGACAAAGACAG+TGG | 0.712149 | 1:-80640303 | None:intergenic |
| TGAAAGAAAAGACATGAGGG+TGG | 0.725920 | 1:-80640336 | None:intergenic |
| GGTGTTGAACAAGTGCTGAG+AGG | 0.756300 | 1:+80640664 | MsaT005365.1:CDS |
| CAAGTGCTGAGAGGTAACGG+CGG | 0.757236 | 1:+80640673 | MsaT005365.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 80640200 | 80641011 | 80640200 | ID=MsaG005365 |
| Chr1 | mRNA | 80640200 | 80641011 | 80640200 | ID=MsaT005365.1;Parent=MsaG005365 |
| Chr1 | exon | 80640200 | 80640383 | 80640200 | ID=MsaT005365.1.exon1;Parent=MsaT005365.1 |
| Chr1 | CDS | 80640200 | 80640383 | 80640200 | ID=cds.MsaT005365.1;Parent=MsaT005365.1 |
| Chr1 | exon | 80640560 | 80641011 | 80640560 | ID=MsaT005365.1.exon2;Parent=MsaT005365.1 |
| Chr1 | CDS | 80640560 | 80641011 | 80640560 | ID=cds.MsaT005365.1;Parent=MsaT005365.1 |
| Gene Sequence |
| Protein sequence |
