AlfalfaGEDB | MsaG005509

M. sativa ssp. caerulea / MsaG005509

KEGG: K11644
GO: -
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005509	XP_004496806.1	51.250	80	35	2	7	85	11	87	1.82e-11	75.5
MsaG005509	XP_004496806.1	54.545	220	93	3	4	216	183	402	1.28e-69	235

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005509	sp\|Q9SRH9\|SNL1_ARATH	43.137	102	41	3	21	121	61	146	4.09e-14	75.1

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005509	tr\|A0A1S2XZ63\|A0A1S2XZ63_CICAR	51.250	80	35	2	7	85	11	87	8.71e-12	75.5
MsaG005509	tr\|A0A1S2XZ63\|A0A1S2XZ63_CICAR	54.545	220	93	3	4	216	183	402	6.13e-70	235

TFs/TRs:

Gene ID	Type	Classification
MsaG005509	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005509	MtrunA17_Chr7g0252101	54.167	216	83	6	1	212	1	204	1.86e-68	217
MsaG005509	MtrunA17_Chr4g0075861	54.902	204	84	3	19	215	174	376	7.88e-63	201
MsaG005509	MtrunA17_Chr4g0075861	42.045	88	49	1	7	94	2	87	1.07e-14	72.8
MsaG005509	MtrunA17_Chr4g0075971	55.882	204	82	3	20	216	175	377	5.19e-62	204
MsaG005509	MtrunA17_Chr4g0075971	42.045	88	49	1	7	94	2	87	9.48e-15	73.2
MsaG005509	MtrunA17_Chr3g0093911	69.466	131	38	2	92	220	1	131	9.04e-54	177
MsaG005509	MtrunA17_Chr1g0196231	69.767	129	34	3	89	216	224	348	3.64e-53	176
MsaG005509	MtrunA17_Chr6g0488101	43.725	247	105	8	20	238	11	251	4.79e-53	177
MsaG005509	MtrunA17_Chr6g0488271	45.161	217	93	6	42	238	1	211	2.26e-50	169
MsaG005509	MtrunA17_Chr3g0077801	58.065	124	52	0	92	215	1	124	2.28e-46	157
MsaG005509	MtrunA17_Chr1g0206951	59.350	123	49	1	95	216	2	124	2.21e-44	145
MsaG005509	MtrunA17_Chr1g0207351	63.934	122	39	2	96	212	16	137	2.65e-43	150
MsaG005509	MtrunA17_Chr3g0077901	52.083	144	68	1	88	231	17	159	3.39e-42	147
MsaG005509	MtrunA17_Chr1g0209201	79.518	83	17	0	2	84	1	83	5.80e-42	140
MsaG005509	MtrunA17_Chr3g0122991	59.016	122	48	2	94	215	2	121	1.25e-40	140
MsaG005509	MtrunA17_Chr3g0077851	55.652	115	50	1	108	222	1	114	4.38e-36	130
MsaG005509	MtrunA17_Chr3g0112311	51.200	125	61	0	92	216	1	125	4.17e-32	120
MsaG005509	MtrunA17_Chr7g0243331	52.941	119	55	1	97	215	6	123	1.41e-30	116
MsaG005509	MtrunA17_Chr7g0243391	48.438	128	63	2	88	215	14	138	9.42e-27	103
MsaG005509	MtrunA17_Chr1g0209061	46.970	132	44	5	1	131	1	107	1.01e-25	97.4
MsaG005509	MtrunA17_Chr3g0077861	49.412	85	38	3	2	81	72	156	1.10e-16	75.1
MsaG005509	MtrunA17_Chr3g0077911	49.412	85	38	3	2	81	72	156	1.16e-16	75.1
MsaG005509	MtrunA17_Chr8g0364231	39.669	121	57	3	21	140	46	151	8.57e-16	76.6
MsaG005509	MtrunA17_Chr6g0452001	41.667	108	47	3	20	126	66	158	1.00e-15	76.6
MsaG005509	MtrunA17_Chr6g0452021	39.024	123	55	3	20	140	46	150	1.16e-15	75.9
MsaG005509	MtrunA17_Chr2g0328701	40.909	88	50	1	7	94	19	104	2.37e-15	70.5
MsaG005509	MtrunA17_Chr1g0206771	49.296	71	35	1	14	83	92	162	8.72e-15	70.5
MsaG005509	MtrunA17_Chr1g0206771	54.098	61	27	1	20	79	19	79	9.02e-13	65.1
MsaG005509	MtrunA17_Chr5g0415031	42.708	96	51	2	5	96	31	126	9.58e-15	73.6
MsaG005509	MtrunA17_Chr3g0077881	41.304	92	44	3	2	83	76	167	3.23e-14	68.6
MsaG005509	MtrunA17_Chr3g0077881	45.205	73	38	1	15	85	6	78	2.41e-11	60.8
MsaG005509	MtrunA17_Chr7g0218911	46.575	73	38	1	21	92	2	74	2.16e-13	65.1
MsaG005509	MtrunA17_Chr1g0206781	49.231	65	32	1	20	83	14	78	2.18e-13	65.1
MsaG005509	MtrunA17_Chr3g0077941	50.000	66	31	1	20	83	101	166	2.39e-13	66.2
MsaG005509	MtrunA17_Chr3g0077941	48.276	58	30	0	28	85	21	78	9.29e-11	59.3
MsaG005509	MtrunA17_Chr7g0238781	42.391	92	50	3	124	215	27	115	7.38e-13	64.7
MsaG005509	MtrunA17_Chr1g0205811	36.000	125	53	4	12	125	4	112	1.12e-12	63.2
MsaG005509	MtrunA17_Chr3g0077811	45.205	73	38	1	15	85	11	83	2.96e-12	62.0
MsaG005509	MtrunA17_Chr3g0077891	48.485	66	32	1	20	83	102	167	3.52e-12	63.2
MsaG005509	MtrunA17_Chr3g0077891	45.205	73	38	1	15	85	6	78	3.09e-11	60.5
MsaG005509	MtrunA17_Chr1g0205821	33.673	98	60	2	2	94	80	177	8.66e-12	62.0
MsaG005509	MtrunA17_Chr1g0205861	41.463	82	42	2	20	100	19	95	3.19e-11	59.3
MsaG005509	MtrunA17_Chr1g0209051	69.767	43	13	0	173	215	1	43	4.03e-11	61.6
MsaG005509	MtrunA17_Chr5g0431721	43.590	78	38	2	2	77	1	74	8.79e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005509	AT3G01320.2	43.137	102	41	3	21	121	61	146	4.15e-15	75.1
MsaG005509	AT3G01320.1	43.137	102	41	3	21	121	61	146	4.15e-15	75.1
MsaG005509	AT1G59890.1	50.667	75	36	1	21	94	49	123	2.48e-14	72.8
MsaG005509	AT1G59890.3	50.667	75	36	1	21	94	49	123	2.50e-14	72.8
MsaG005509	AT1G59890.2	50.667	75	36	1	21	94	49	123	2.50e-14	72.8
MsaG005509	AT1G59890.4	50.667	75	36	1	21	94	49	123	2.57e-14	72.8
MsaG005509	AT5G15020.2	35.537	121	60	2	21	140	56	159	4.93e-14	72.0
MsaG005509	AT5G15020.1	35.537	121	60	2	21	140	56	159	5.13e-14	72.0
MsaG005509	AT1G24190.3	37.037	108	52	2	20	126	17	109	3.00e-13	69.7
MsaG005509	AT1G24190.1	37.037	108	52	2	20	126	17	109	3.11e-13	69.7
MsaG005509	AT1G24190.2	37.037	108	52	2	20	126	17	109	3.14e-13	69.7
MsaG005509	AT1G70060.2	49.254	67	33	1	20	85	17	83	5.55e-12	65.9
MsaG005509	AT1G70060.1	49.254	67	33	1	20	85	17	83	5.55e-12	65.9
MsaG005509	AT4G12020.1	42.857	70	40	0	16	85	307	376	3.06e-11	63.5
MsaG005509	AT4G12020.9	42.857	70	40	0	16	85	307	376	3.06e-11	63.5
MsaG005509	AT4G12020.7	42.857	70	40	0	16	85	307	376	3.21e-11	63.5
MsaG005509	AT4G12020.4	42.857	70	40	0	16	85	307	376	3.22e-11	63.5
MsaG005509	AT4G12020.2	42.857	70	40	0	16	85	307	376	3.26e-11	63.5
MsaG005509	AT4G12020.3	42.857	70	40	0	16	85	307	376	3.31e-11	63.5
MsaG005509	AT4G12020.10	42.857	70	40	0	16	85	307	376	3.34e-11	63.5
MsaG005509	AT4G12020.6	42.857	70	40	0	16	85	307	376	3.36e-11	63.5
MsaG005509	AT4G12020.8	42.857	70	40	0	16	85	307	376	3.36e-11	63.5
MsaG005509	AT4G12020.5	42.857	70	40	0	16	85	240	309	3.37e-11	63.5
MsaG005509	AT4G12020.11	42.857	70	40	0	16	85	307	376	3.50e-11	63.5
MsaG005509	AT5G15025.1	40.476	84	48	2	5	86	34	117	6.32e-11	58.9

Find 42 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGGCATACTCATTTAATTT+TGG	0.100116	1:-82216840	MsaT005509.1:CDS
TAAAACTTCTAAAAGATTTC+AGG	0.175218	1:-82216916	MsaT005509.1:CDS
ACCTTCTTTATCCTTGTAAA+TGG	0.175296	1:+82212613	None:intergenic
AGAAGACTCGTCATTGTTTC+GGG	0.206361	1:+82217018	None:intergenic
AATATATTGGAGAGATTTCT+TGG	0.244304	1:-82212824	MsaT005509.1:CDS
CTTGAAGAAGTAATGGTTCT+TGG	0.275926	1:+82212797	None:intergenic
TAGAAGACTCGTCATTGTTT+CGG	0.323320	1:+82217017	None:intergenic
GACGACCTCATTCAGTTTGC+CGG	0.346455	1:-82212877	MsaT005509.1:CDS
CTTCTTCAAGTTTCGTATCA+TGG	0.349566	1:-82212784	MsaT005509.1:CDS
AGAGTGAAGAGGCTGTTTAA+AGG	0.366179	1:-82216860	MsaT005509.1:CDS
GTCGTCAAAATCTAGTGTTT+TGG	0.396017	1:+82212894	None:intergenic
TTTGTCGGACATGCGAATAA+AGG	0.397536	1:+82212747	None:intergenic
AGTTTGATTTGATGTTCCTT+TGG	0.399942	1:+82216800	None:intergenic
CAAGTTCTTTCATGTTGGAT+TGG	0.402076	1:-82211952	MsaT005509.1:CDS
GACGAGTCTTCTAGAGTTTG+CGG	0.406561	1:-82217007	MsaT005509.1:CDS
TTGAAGAAGTAATGGTTCTT+GGG	0.415263	1:+82212798	None:intergenic
GTTGTGATCTAAAGGTAAAA+AGG	0.421826	1:-82212971	MsaT005509.1:intron
TAGAGTTTGCGGCAATGTAT+CGG	0.422180	1:-82216996	MsaT005509.1:CDS
TTGCCGGTGTGTGCAAGAAT+TGG	0.432034	1:-82212861	MsaT005509.1:CDS
GAGTGAAGAGGCTGTTTAAA+GGG	0.456342	1:-82216859	MsaT005509.1:CDS
AGAGTTTGCGGCAATGTATC+GGG	0.456472	1:-82216995	MsaT005509.1:CDS
CTTCTAAAAGATTTCAGGAC+TGG	0.463048	1:-82216911	MsaT005509.1:CDS
TGCTATTCAATCCATTTACA+AGG	0.472650	1:-82212624	MsaT005509.1:CDS
AAACTTGTTTCATGAGGAGC+AGG	0.478813	1:+82211986	None:intergenic
TTGATGTTCCTTTGGCAGAA+AGG	0.479184	1:+82216808	None:intergenic
ATGAGATCAAAGACATCTAA+AGG	0.482617	1:+82212919	None:intergenic
ACTTATGTTGCGTCGTCTAG+TGG	0.484912	1:-82212682	MsaT005509.1:CDS
TGAAACAAGTTCTTTCATGT+TGG	0.491534	1:-82211957	MsaT005509.1:CDS
ACTGGAAGAATTGATATAAG+AGG	0.527602	1:-82216893	MsaT005509.1:CDS
TCCATTTACAAGGATAAAGA+AGG	0.529988	1:-82212614	MsaT005509.1:intron
CCGGTGTGTGCAAGAATTGG+AGG	0.534247	1:-82212858	MsaT005509.1:CDS
TACGAAACTTGAAGAAGTAA+TGG	0.554712	1:+82212790	None:intergenic
ATTCAACGCCTTTCTGCCAA+AGG	0.569612	1:-82216816	MsaT005509.1:CDS
TGAGAGAAACTTGTTTCATG+AGG	0.575659	1:+82211980	None:intergenic
ACACACCGGCAAACTGAATG+AGG	0.595057	1:+82212872	None:intergenic
AAGACTCGTCATTGTTTCGG+GGG	0.611127	1:+82217020	None:intergenic
GTGTCAAAGCAAGAGTGAAG+AGG	0.613812	1:-82216871	MsaT005509.1:CDS
CGGTGTGTGCAAGAATTGGA+GGG	0.623193	1:-82212857	MsaT005509.1:CDS
CAAATCAAACTTGAGACCAC+CGG	0.630760	1:-82216788	MsaT005509.1:intron
TAGACGACGCAACATAAGTA+CGG	0.641936	1:+82212686	None:intergenic
GAAGACTCGTCATTGTTTCG+GGG	0.644759	1:+82217019	None:intergenic
CCTCCAATTCTTGCACACAC+CGG	0.651110	1:+82212858	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	82211924	82217161	82211924	ID=MsaG005509
Chr1	mRNA	82211924	82217161	82211924	ID=MsaT005509.1;Parent=MsaG005509
Chr1	exon	82211924	82212043	82211924	ID=MsaT005509.1.exon4;Parent=MsaT005509.1
Chr1	CDS	82211924	82212043	82211924	ID=cds.MsaT005509.1;Parent=MsaT005509.1
Chr1	exon	82212615	82212979	82212615	ID=MsaT005509.1.exon3;Parent=MsaT005509.1
Chr1	CDS	82212615	82212979	82212615	ID=cds.MsaT005509.1;Parent=MsaT005509.1
Chr1	exon	82216789	82217050	82216789	ID=MsaT005509.1.exon2;Parent=MsaT005509.1
Chr1	CDS	82216789	82217050	82216789	ID=cds.MsaT005509.1;Parent=MsaT005509.1
Chr1	exon	82217159	82217161	82217159	ID=MsaT005509.1.exon1;Parent=MsaT005509.1
Chr1	CDS	82217159	82217161	82217159	ID=cds.MsaT005509.1;Parent=MsaT005509.1

Gene Sequence

>MsaT005509.1

ATGATGAAGATCCCCCGAAACAATGACGAGTCTTCTAGAGTTTGCGGCAATGTATCGGGTTATTTTGAGGAAGTAAATGCTGCATTTCAATATAACAAAAAGTATTTTCAGTTTATAAAACTTCTAAAAGATTTCAGGACTGGAAGAATTGATATAAGAGGTGTCAAAGCAAGAGTGAAGAGGCTGTTTAAAGGGCATACTCATTTAATTTTGGGATTCAACGCCTTTCTGCCAAAGGAACATCAAATCAAACTTGAGACCACCGGTAAAAAGGTGAAGAAGGTAGAAGAGAACTGTGAACTTCCTTTAGATGTCTTTGATCTCATTTCCAAAACACTAGATTTTGACGACCTCATTCAGTTTGCCGGTGTGTGCAAGAATTGGAGGGCTTTTCATAAAATATATTGGAGAGATTTCTTGGCATCCCAAGAACCATTACTTCTTCAAGTTTCGTATCATGGTAATGAATCATATTCCTTTATTCGCATGTCCGACAAAAAAATTTATTGCTTGAAGATGATGAAGTACTTTGAGTACCGTACTTATGTTGCGTCGTCTAGTGGATATTTTATCATGGTGGGGGACAATAATTCATTTATGCTATTCAATCCATTTACAAGGATAAAGAAGGTGATGATAAAAAGTTGCAAAAATACATTAAAATTCCTGCTCCTCATGAAACAAGTTTCTCTCATGAAACAAGTTCTTTCATGTTGGATTGGTGTTTTAGACATCTACAATTTGAGGTAG

Protein sequence

>MsaT005509.1

MMKIPRNNDESSRVCGNVSGYFEEVNAAFQYNKKYFQFIKLLKDFRTGRIDIRGVKARVKRLFKGHTHLILGFNAFLPKEHQIKLETTGKKVKKVEENCELPLDVFDLISKTLDFDDLIQFAGVCKNWRAFHKIYWRDFLASQEPLLLQVSYHGNESYSFIRMSDKKIYCLKMMKYFEYRTYVASSSGYFIMVGDNNSFMLFNPFTRIKKVMIKSCKNTLKFLLLMKQVSLMKQVLSCWIGVLDIYNLR*