AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005732

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005732 MtrunA17_Chr1g0211711 81.495 1097 155 8 1 1087 1 1059 0.0 1743
MsaG005732 MtrunA17_Chr1g0183311 84.100 761 109 4 339 1087 130 890 0.0 1303
MsaG005732 MtrunA17_Chr1g0211691 65.323 992 225 12 7 967 42 945 0.0 1187
MsaG005732 MtrunA17_Chr5g0426961 72.386 746 164 4 337 1080 131 836 0.0 1093
MsaG005732 MtrunA17_Chr1g0211661 59.793 868 291 8 157 1019 51 865 0.0 1004
MsaG005732 MtrunA17_Chr1g0211661 51.449 138 54 4 7 131 42 179 1.04e-14 79.3
MsaG005732 MtrunA17_Chr5g0420361 68.563 668 187 10 410 1072 139 788 0.0 907
MsaG005732 MtrunA17_Chr6g0472441 65.030 672 184 13 410 1072 81 710 0.0 851
MsaG005732 MtrunA17_Chr7g0275771 42.996 928 436 21 225 1085 32 933 0.0 694
MsaG005732 MtrunA17_Chr4g0075261 46.817 754 335 15 339 1076 161 864 0.0 648
MsaG005732 MtrunA17_Chr5g0448301 56.923 585 190 5 409 989 105 631 0.0 643
MsaG005732 MtrunA17_Chr2g0289121 47.476 733 314 13 346 1076 156 819 0.0 623
MsaG005732 MtrunA17_Chr1g0192011 35.569 835 448 19 269 1077 191 961 4.99e-146 463
MsaG005732 MtrunA17_Chr2g0289031 53.613 429 153 4 651 1076 8 393 5.32e-142 432
MsaG005732 MtrunA17_Chr1g0147021 45.224 513 236 10 340 843 864 1340 7.83e-132 439
MsaG005732 MtrunA17_Chr1g0147021 75.000 136 34 0 932 1067 1551 1686 3.28e-60 226
MsaG005732 MtrunA17_Chr2g0281051 34.282 773 451 15 346 1081 207 959 3.63e-129 417
MsaG005732 MtrunA17_Chr8g0340381 73.256 172 26 4 746 916 36 188 3.87e-68 226
MsaG005732 MtrunA17_Chr1g0211701 79.675 123 25 0 965 1087 4 126 1.52e-67 223
MsaG005732 MtrunA17_Chr8g0364421 49.219 256 98 4 456 710 5 229 8.79e-66 221
MsaG005732 MtrunA17_Chr8g0361381 54.011 187 82 2 647 833 26 208 1.07e-59 203
MsaG005732 MtrunA17_Chr4g0048531 39.731 297 168 5 567 859 727 1016 1.92e-58 220
MsaG005732 MtrunA17_Chr4g0048531 57.246 138 58 1 931 1068 1132 1268 4.84e-44 174
MsaG005732 MtrunA17_Chr4g0048531 33.690 187 90 4 346 511 260 433 3.97e-27 119
MsaG005732 MtrunA17_Chr7g0231061 58.960 173 68 1 705 877 8 177 7.68e-58 198
MsaG005732 MtrunA17_Chr2g0289091 75.676 111 27 0 931 1041 310 420 3.42e-51 186
MsaG005732 MtrunA17_Chr2g0289091 58.741 143 58 1 725 867 23 164 1.08e-47 176
MsaG005732 MtrunA17_Chr8g0361391 76.404 89 20 1 931 1019 50 137 4.53e-42 151
MsaG005732 MtrunA17_Chr2g0289041 42.029 207 103 5 368 569 2 196 9.77e-42 152
MsaG005732 MtrunA17_Chr8g0364411 58.036 112 47 0 346 457 33 144 1.58e-37 137
MsaG005732 MtrunA17_Chr1g0211671 75.806 62 15 0 1018 1079 15 76 2.85e-27 107
MsaG005732 MtrunA17_Chr3g0119761 57.143 77 32 1 819 894 55 131 1.95e-17 79.7
MsaG005732 MtrunA17_Chr2g0289101 35.115 131 72 5 478 603 8 130 3.56e-14 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005732 AT3G07610.1 47.188 818 334 9 316 1082 127 897 0.0 731
MsaG005732 AT3G07610.3 45.878 837 339 10 316 1082 127 919 0.0 712
MsaG005732 AT1G11950.1 48.985 739 314 12 342 1076 189 868 0.0 680
MsaG005732 AT1G11950.3 48.985 739 314 12 342 1076 189 868 0.0 680
MsaG005732 AT1G62310.1 48.693 727 316 12 345 1065 208 883 0.0 669
MsaG005732 AT4G00990.1 44.159 779 356 15 346 1075 80 828 0.0 618
MsaG005732 AT1G11950.2 46.726 672 295 12 342 1009 189 801 0.0 575
MsaG005732 AT4G00990.3 42.798 729 338 15 346 1025 80 778 0.0 549
MsaG005732 AT4G00990.2 43.135 721 331 15 346 1017 80 770 1.56e-180 546
MsaG005732 AT3G07610.2 45.043 575 254 7 316 839 127 690 4.98e-156 482
MsaG005732 AT1G09060.2 32.357 819 451 21 283 1077 152 891 9.09e-121 394
MsaG005732 AT1G09060.1 32.357 819 451 21 283 1077 152 891 9.09e-121 394
MsaG005732 AT1G09060.4 32.357 819 451 21 283 1077 166 905 1.50e-120 394
MsaG005732 AT1G09060.3 32.357 819 451 21 283 1077 166 905 1.50e-120 394
MsaG005732 AT4G21430.1 29.566 761 457 15 340 1083 221 919 9.53e-98 332

Find 255 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATTTAAAGAAGCATTTC+AGG 0.172351 1:-84689172 MsaT005732.1:CDS
GCTTGATCTGGTCAAGAATT+TGG 0.181519 1:-84680900 MsaT005732.1:CDS
AGTACATTGCTGACAATTAT+AGG 0.182829 1:+84690886 None:intergenic
GGAGGAGGATTTGAAGAAAA+AGG 0.184311 1:-84693055 MsaT005732.1:CDS
GGATCTGTATATTCTTTAAA+TGG 0.212005 1:+84689912 None:intergenic
TCATATTGCTGAAGTAAAAT+TGG 0.226978 1:-84689501 MsaT005732.1:CDS
CGCTAGTTTCAGTTCCTTAA+TGG 0.250548 1:+84689460 None:intergenic
CTTGACCATTCACGAATCTT+AGG 0.252597 1:+84691261 None:intergenic
TTTCTACGGCGTTTCTTTGC+CGG 0.256532 1:+84693210 None:intergenic
AATGTGCACCAATTCTTTAC+TGG 0.265399 1:-84690059 MsaT005732.1:CDS
CTCCGTGGTGGGAAGCTTCT+TGG 0.267716 1:-84691390 MsaT005732.1:CDS
TACCAAACACAAAACACTTT+TGG 0.267787 1:-84690797 MsaT005732.1:CDS
GGAAGATTACGTTTCAGATT+TGG 0.280245 1:-84693076 MsaT005732.1:CDS
TAAAGGAATTGATGCCATTA+AGG 0.292245 1:-84689474 MsaT005732.1:CDS
CTGACATTGATGGCACATTA+AGG 0.293064 1:+84692507 None:intergenic
AGTTGCAAATTACCCTAAAA+AGG 0.307588 1:-84691586 MsaT005732.1:CDS
TTTAAATATTCCTCCAACTT+AGG 0.317362 1:+84689185 None:intergenic
ATTCTTTAGATGGTGCTCTC+TGG 0.323154 1:-84689232 MsaT005732.1:CDS
GCATACCTTGTTTAAACAAT+TGG 0.324245 1:-84692429 MsaT005732.1:intron
ACTCCCTCTTGTCTTGCTTA+AGG 0.325852 1:+84689435 None:intergenic
TCATCCAACCGTCTTAAATA+TGG 0.332074 1:+84691965 None:intergenic
TCTGGCTGTAGAACAGATTT+AGG 0.332318 1:+84689855 None:intergenic
TCTGCAGACCTGTGATTTAC+TGG 0.334746 1:+84681446 None:intergenic
TGTCATTTCTCTGACATTGA+TGG 0.335478 1:+84692497 None:intergenic
AGAAGCATTTCAGGGAGTTT+AGG 0.336892 1:-84689163 MsaT005732.1:CDS
TGTGATTTACTGGAAGTTTA+CGG 0.345470 1:+84681456 None:intergenic
GTCGCCTGGATTGGCTCAAC+TGG 0.348516 1:-84690025 MsaT005732.1:CDS
AACGTTCAAGTCGTGGAAAA+TGG 0.360091 1:-84689329 MsaT005732.1:CDS
GTACTTTCTTATCACTTTAA+CGG 0.361591 1:+84693398 None:intergenic
CGAAGACAACAATCAATAGA+TGG 0.363009 1:-84692654 MsaT005732.1:CDS
GCCAAGTCAACAACATTTAA+CGG 0.375653 1:+84693594 None:intergenic
TGATAAGAAAGTACGGTAGA+AGG 0.376996 1:-84693389 MsaT005732.1:CDS
GCCGATGATGTTTGTGATCA+TGG 0.377605 1:-84691192 MsaT005732.1:CDS
AAGAGGCTTAAGGAGGAGTA+TGG 0.378678 1:-84682881 MsaT005732.1:intron
ACGGAGAAGGAAAATGAAAC+AGG 0.382359 1:-84693180 MsaT005732.1:CDS
GATGGTGCTGAGGTGGAAGG+AGG 0.385781 1:-84693156 MsaT005732.1:CDS
CTCAATCTCATGGTCGTTGT+TGG 0.385898 1:+84692018 None:intergenic
GAAGTATGCTTCTCAGTTCC+AGG 0.387106 1:+84691166 None:intergenic
TATGGATTTGCTCAGGAGCT+TGG 0.388333 1:-84689807 MsaT005732.1:CDS
AAAACTTCTTATACTGTCCT+AGG 0.392719 1:-84690969 MsaT005732.1:CDS
GGGAGTGGCATTGTGATAAT+CGG 0.395603 1:-84689418 MsaT005732.1:CDS
ATTTGCTCAGGAGCTTGGGC+GGG 0.402390 1:-84689802 MsaT005732.1:CDS
AAGCTGCCTTCCTTGTGTTA+TGG 0.405440 1:+84691010 None:intergenic
CGCCGATACGGCGTCGTTGA+AGG 0.405565 1:-84693553 MsaT005732.1:CDS
TTCAATGGCACTGGAGCAAA+GGG 0.413215 1:-84690912 MsaT005732.1:CDS
TCTTCTTATGCTCGATAGTC+AGG 0.416415 1:+84682900 None:intergenic
TTCTTTAGATGGTGCTCTCT+GGG 0.418384 1:-84689231 MsaT005732.1:CDS
TTTGCTCAGGAGCTTGGGCG+GGG 0.418489 1:-84689801 MsaT005732.1:CDS
ATGGATTTGCTCAGGAGCTT+GGG 0.418850 1:-84689806 MsaT005732.1:CDS
AATGTACTTGAATGTACATC+TGG 0.421952 1:-84690871 MsaT005732.1:CDS
GGATTGGCTCAACTGGCCTC+AGG 0.422520 1:-84690018 MsaT005732.1:CDS
CTGGTGGCGCTGATGATGAT+GGG 0.426414 1:-84689259 MsaT005732.1:CDS
GGAGATAGAAGCAAAGAGAC+AGG 0.430402 1:-84691924 MsaT005732.1:CDS
TATGAACTCGGCACAATGAC+GGG 0.431394 1:+84689944 None:intergenic
TACCATTTACACTGGAACCT+TGG 0.432048 1:+84680667 None:intergenic
TTGTGTACCCAGTAAAGAAT+TGG 0.434568 1:+84690051 None:intergenic
TCACAAACATCATCGGCATT+TGG 0.438525 1:+84691198 None:intergenic
CCACCAGAGTGTTCATGAAC+TGG 0.444158 1:+84689275 None:intergenic
CGAGCATAAGAAGAGGCTTA+AGG 0.446517 1:-84682891 MsaT005732.1:CDS
AATGTACATCTGGTTTAAGC+TGG 0.447849 1:-84690861 MsaT005732.1:CDS
GAAAGTTAAAAGCAGTGAAC+AGG 0.448634 1:-84692677 MsaT005732.1:CDS
GCATCAAGTTCGAAATCTTA+AGG 0.453263 1:-84682634 MsaT005732.1:intron
CAGGTCTGCAGAGGACAAAT+TGG 0.455837 1:-84681435 MsaT005732.1:CDS
GGAAATTGCCGAGGCATGTC+CGG 0.456004 1:-84692197 MsaT005732.1:CDS
AACAAAATCAAGCGCAACCT+TGG 0.459482 1:+84681523 None:intergenic
TGTTTGTGATCATGGTTTCC+TGG 0.459683 1:-84691184 MsaT005732.1:CDS
AACATCATCGGCATTTGGAC+AGG 0.460874 1:+84691203 None:intergenic
TTCTTTACTGGGTACACAAA+GGG 0.461069 1:-84690047 MsaT005732.1:CDS
TGAATAACCTTTATTATCAT+CGG 0.461347 1:+84692720 None:intergenic
ATGGTGCTGAGGTGGAAGGA+GGG 0.462347 1:-84693155 MsaT005732.1:CDS
GCCTCCGTCTAATTTATTTG+AGG 0.463495 1:-84689976 MsaT005732.1:CDS
TCTGGTGGCGCTGATGATGA+TGG 0.466251 1:-84689260 MsaT005732.1:CDS
ACACAAAGGGTCGCCTGGAT+TGG 0.466392 1:-84690034 MsaT005732.1:CDS
AATATAAATTCGAACTCGAT+TGG 0.467121 1:+84691357 None:intergenic
TGGTGAGTGCTTTCGTTTGA+CGG 0.467146 1:-84681486 MsaT005732.1:CDS
ACCATTCACGAATCTTAGGT+TGG 0.473569 1:+84691265 None:intergenic
TATATATGCTATGCTTGATC+TGG 0.473768 1:-84680912 MsaT005732.1:CDS
AGAAGAAACCTGGTGGGAAA+AGG 0.477597 1:-84692945 MsaT005732.1:CDS
TGGGTACACAAAGGGTCGCC+TGG 0.477763 1:-84690039 MsaT005732.1:CDS
TGAGGAAGAGTGAAAGGCCT+AGG 0.478067 1:-84693116 MsaT005732.1:CDS
TCTGCCATATTTAAGACGGT+TGG 0.481699 1:-84691969 MsaT005732.1:CDS
AAGTTGTTTCAGCATATACT+TGG 0.482851 1:+84691991 None:intergenic
GTCAGAGAAATGACAAGGGC+AGG 0.483009 1:-84692489 MsaT005732.1:CDS
AGAAAATGATGATGAACAAG+AGG 0.483029 1:-84692818 MsaT005732.1:CDS
AAGCCTGTTGTGAATGCATC+TGG 0.486125 1:-84691039 MsaT005732.1:CDS
TATTGCTTATGGATTTGCTC+AGG 0.486399 1:-84689814 MsaT005732.1:CDS
AGGCAATTGATTGCTTAGAT+TGG 0.488744 1:-84690768 MsaT005732.1:CDS
GAGAGAGGATGTACCTAAGT+TGG 0.490798 1:-84689198 MsaT005732.1:CDS
ATGTGCACCAATTCTTTACT+GGG 0.492244 1:-84690058 MsaT005732.1:CDS
GATTTGCTCAGGAGCTTGGG+CGG 0.493090 1:-84689803 MsaT005732.1:CDS
ACGGTTGGATGAGGAGCAAA+TGG 0.494689 1:-84691954 MsaT005732.1:CDS
ATTGATCAGTGAAAAGAATC+CGG 0.496746 1:-84692758 MsaT005732.1:CDS
TGAGGAGCAAATGGCTGAGA+AGG 0.497473 1:-84691945 MsaT005732.1:CDS
GCGATCATGTGTACTTATTG+AGG 0.498139 1:-84692140 MsaT005732.1:intron
ATTCGTGAATGGTCAAGATC+TGG 0.498187 1:-84691255 MsaT005732.1:CDS
TAGGAAGATTAAATCGTTGA+AGG 0.499461 1:-84693097 MsaT005732.1:CDS
AACGATTTAGCTTCCGTCGA+AGG 0.501873 1:-84693450 MsaT005732.1:CDS
GAAGTAAAATTGGAAACTAA+AGG 0.501982 1:-84689491 MsaT005732.1:CDS
GAGGCCAGTTGAGCCAATCC+AGG 0.502273 1:+84690021 None:intergenic
GAAGGTGATGACGTGGCAGC+CGG 0.504006 1:-84693432 MsaT005732.1:CDS
CCATTCACGAATCTTAGGTT+GGG 0.504995 1:+84691266 None:intergenic
ATACCAGATGCATTCACAAC+AGG 0.505405 1:+84691036 None:intergenic
CGTGAATGTTGTCGCCGATA+CGG 0.505413 1:-84693565 MsaT005732.1:CDS
GAAAATGATGATGAACAAGA+GGG 0.506201 1:-84692817 MsaT005732.1:CDS
AGATTACGTTTCAGATTTGG+AGG 0.506724 1:-84693073 MsaT005732.1:CDS
TTCAACGATTTAATCTTCCT+AGG 0.509264 1:+84693099 None:intergenic
CAGAGGACACATCTAATAGT+AGG 0.511755 1:-84691074 MsaT005732.1:CDS
TGTATATTCTTTAAATGGCA+AGG 0.514940 1:+84689917 None:intergenic
TTTCAATGGCACTGGAGCAA+AGG 0.515097 1:-84690913 MsaT005732.1:CDS
GGTAAGAAGAAGGTGATAAT+CGG 0.516548 1:-84693024 MsaT005732.1:CDS
GGAGTGGCATTGTGATAATC+GGG 0.518092 1:-84689417 MsaT005732.1:CDS
TGGTAGAAATGTTAAGATTG+AGG 0.519416 1:-84692788 MsaT005732.1:CDS
GCCGTTAAATGTTGTTGACT+TGG 0.521416 1:-84693595 MsaT005732.1:CDS
CGTGGTGGGAAGCTTCTTGG+TGG 0.522591 1:-84691387 MsaT005732.1:CDS
GGGGAAGCGTGGTAAGAAGA+AGG 0.524382 1:-84693034 MsaT005732.1:CDS
GCAAAATAACGTTCAAGTCG+TGG 0.530405 1:-84689336 MsaT005732.1:CDS
GTCCTTCAACGACGCCGTAT+CGG 0.531655 1:+84693551 None:intergenic
TCAGGTAAAAGTGCTGATCA+TGG 0.534050 1:+84682918 None:intergenic
ATGGAAGAAGCGTTTGATAA+CGG 0.534596 1:-84693318 MsaT005732.1:CDS
ATATTTAAGACGGTTGGATG+AGG 0.536491 1:-84691963 MsaT005732.1:CDS
TGGATTATGTGATGCTAAAG+TGG 0.537080 1:-84689303 MsaT005732.1:CDS
ATCTGTTCTACAGCCAGACA+TGG 0.537266 1:-84689850 MsaT005732.1:CDS
CGTATCGGCGACAACATTCA+CGG 0.537504 1:+84693566 None:intergenic
AATGACGCTGCAGAAGAAGA+AGG 0.537607 1:-84692988 MsaT005732.1:CDS
ATATTTAAAGAAGCATTTCA+GGG 0.537995 1:-84689171 MsaT005732.1:CDS
CCATTGCTCTCCTTTAAAGC+AGG 0.538406 1:-84689135 MsaT005732.1:intron
AACTCGGAGATGCAATTTCG+TGG 0.538906 1:+84691144 None:intergenic
ACAGCACACCGGACATGCCT+CGG 0.539816 1:+84692189 None:intergenic
GATCCATTTACCATTTACAC+TGG 0.540430 1:+84680659 None:intergenic
ACTTGATGCGGACAACCAGC+TGG 0.540777 1:+84682648 None:intergenic
TGGTGGCGCTGATGATGATG+GGG 0.542939 1:-84689258 MsaT005732.1:CDS
CACGGAGCTCGCAGCAACAA+AGG 0.543204 1:+84691406 None:intergenic
TTTAACGGAAGAAGAATCTC+CGG 0.544880 1:+84693413 None:intergenic
ACAAAGGAAGGAACGAGTAA+AGG 0.549736 1:-84693294 MsaT005732.1:CDS
AATCAATAGATGGGATCCTA+AGG 0.550030 1:-84692644 MsaT005732.1:intron
GTAAAAGTGCTGATCATGGA+TGG 0.552902 1:+84682922 None:intergenic
TTTGTTTCTCCTGAAAATGT+TGG 0.552946 1:-84681506 MsaT005732.1:CDS
TGAAATCGAGAAGGAGAAGA+TGG 0.556257 1:-84693337 MsaT005732.1:CDS
AGAGGATGTACCTAAGTTGG+AGG 0.557649 1:-84689195 MsaT005732.1:CDS
GGGGATGGTGCTGAGGTGGA+AGG 0.557684 1:-84693159 MsaT005732.1:CDS
TGTTGCTGCGAGCTCCGTGG+TGG 0.558000 1:-84691402 MsaT005732.1:CDS
AGGTTTAGAACACCTTTGAA+AGG 0.559633 1:+84689891 None:intergenic
AACAGGGGATGGTGCTGAGG+TGG 0.560994 1:-84693163 MsaT005732.1:CDS
ATGAACGGAAACAGAGTCTC+CGG 0.561333 1:+84693254 None:intergenic
GACTTGTAGGTATAGAACCT+TGG 0.561487 1:-84682707 MsaT005732.1:intron
CAAGTCGTGGAAAATGGAAG+TGG 0.561682 1:-84689323 MsaT005732.1:CDS
CTTTGCCGGAGTGGTAGCAT+CGG 0.562666 1:+84693224 None:intergenic
AGCATCGGCGGACGCATGAA+CGG 0.563702 1:+84693239 None:intergenic
CATGCAGATAATCACGCCCT+TGG 0.564591 1:+84691334 None:intergenic
ATATGAACTCGGCACAATGA+CGG 0.564749 1:+84689943 None:intergenic
GTAAACTTCCAGTAAATCAC+AGG 0.565327 1:-84681454 MsaT005732.1:CDS
ATGACGCTGCAGAAGAAGAA+GGG 0.566480 1:-84692987 MsaT005732.1:CDS
ATTATCAAGCATGTCAATGT+TGG 0.566654 1:+84689385 None:intergenic
ATGTACATCTGGTTTAAGCT+GGG 0.567172 1:-84690860 MsaT005732.1:CDS
AGTTCGAATTTATATTCCAA+GGG 0.568837 1:-84691350 MsaT005732.1:CDS
CAATTAACAGTGGATTGAAG+AGG 0.569202 1:-84692533 MsaT005732.1:intron
TCGGATAAAACATCACTCTC+CGG 0.569416 1:+84692739 None:intergenic
GTTAAAGTGATAAGAAAGTA+CGG 0.570276 1:-84693396 MsaT005732.1:CDS
ATTTCCTCCTCTTTCAAGTG+AGG 0.570693 1:+84692214 None:intergenic
GTTGCTGCGAGCTCCGTGGT+GGG 0.571109 1:-84691401 MsaT005732.1:CDS
AACTTCTGCCATATTTAAGA+CGG 0.572746 1:-84691973 MsaT005732.1:CDS
GAGTTCGAATTTATATTCCA+AGG 0.572914 1:-84691351 MsaT005732.1:CDS
TCAAGAATTTGGAGAAAGCA+AGG 0.573956 1:-84680889 MsaT005732.1:intron
AAACAACACAGTTACAGAAA+CGG 0.574009 1:-84693619 MsaT005732.1:CDS
GAAGACAACAATCAATAGAT+GGG 0.577671 1:-84692653 MsaT005732.1:CDS
CTTTCCTCAAATAAATTAGA+CGG 0.579101 1:+84689972 None:intergenic
ACGGCGTTTCTTTGCCGGAG+TGG 0.580791 1:+84693215 None:intergenic
GAATATACAGATCCTTTCAA+AGG 0.581672 1:-84689903 MsaT005732.1:CDS
AAATGATGATGAACAAGAGG+GGG 0.582260 1:-84692815 MsaT005732.1:CDS
TTACGTTTCAGATTTGGAGG+AGG 0.582685 1:-84693070 MsaT005732.1:CDS
CAAGGGCGTGATTATCTGCA+TGG 0.583160 1:-84691333 MsaT005732.1:CDS
ACTTGGAGAGCTCAATCTCA+TGG 0.583734 1:+84692008 None:intergenic
CGGAGAAGGAAAATGAAACA+GGG 0.583770 1:-84693179 MsaT005732.1:CDS
AAGGTATCCTCACTTGAAAG+AGG 0.584254 1:-84692221 MsaT005732.1:CDS
CCTGCTTTAAAGGAGAGCAA+TGG 0.584616 1:+84689135 None:intergenic
TTAAGATTTCGAACTTGATG+CGG 0.586896 1:+84682636 None:intergenic
TTGTACCAATTGTTTAAACA+AGG 0.588142 1:+84692424 None:intergenic
TTTGATAACGGAACAAAGGA+AGG 0.589935 1:-84693306 MsaT005732.1:CDS
GCACCTTAAGCAAGACAAGA+GGG 0.593361 1:-84689438 MsaT005732.1:CDS
GCTGGGAACCGCTTGTCATG+TGG 0.594440 1:-84690843 MsaT005732.1:CDS
GGTGATGATGATGTGGCAGC+CGG 0.595870 1:-84693273 MsaT005732.1:CDS
CGGTGAAAGAAGCGATGACG+TGG 0.596772 1:-84693529 MsaT005732.1:CDS
TGCCACGTCATCACCTTCGA+CGG 0.598467 1:+84693437 None:intergenic
GTCTGCAGAGGACAAATTGG+AGG 0.599452 1:-84681432 MsaT005732.1:intron
TGCCGGAGTGGTAGCATCGG+CGG 0.600707 1:+84693227 None:intergenic
GTTTGGTATTGCTTATCTGA+CGG 0.604846 1:+84690812 None:intergenic
CACCAAGAAGCTTCCCACCA+CGG 0.605477 1:+84691388 None:intergenic
TGACATAGATTTACTGCCCT+AGG 0.607035 1:+84690952 None:intergenic
ATTCTTTACTGGGTACACAA+AGG 0.607221 1:-84690048 MsaT005732.1:CDS
TCTTTCAGTTTCAAAACCTG+AGG 0.609559 1:+84690002 None:intergenic
ACCATGATCACAAACATCAT+CGG 0.609796 1:+84691191 None:intergenic
TTAAGCAAGACAAGAGGGAG+TGG 0.610856 1:-84689433 MsaT005732.1:CDS
CCCAACCTAAGATTCGTGAA+TGG 0.610952 1:-84691266 MsaT005732.1:CDS
TACAGGGCTCTCCCTTTCTG+AGG 0.613170 1:-84691619 MsaT005732.1:intron
TAAAAGTGCTGATCATGGAT+GGG 0.614987 1:+84682923 None:intergenic
AATGATGATGAACAAGAGGG+GGG 0.617308 1:-84692814 MsaT005732.1:CDS
CGCACCTTAAGCAAGACAAG+AGG 0.619124 1:-84689439 MsaT005732.1:CDS
AAGTAAGGCTGAAATCGAGA+AGG 0.620239 1:-84693346 MsaT005732.1:CDS
ATTTAGGCAACTTCACAGCA+AGG 0.622999 1:+84689871 None:intergenic
TCAGAGAAATGACAAGGGCA+GGG 0.624776 1:-84692488 MsaT005732.1:CDS
AAAATGATGATGAACAAGAG+GGG 0.624793 1:-84692816 MsaT005732.1:CDS
GAAACCTGGTGGGAAAAGGA+AGG 0.624947 1:-84692941 MsaT005732.1:CDS
CTGGTATACACCATAACACA+AGG 0.626969 1:-84691020 MsaT005732.1:CDS
GAGGCATGTCCGGTGTGCTG+TGG 0.628664 1:-84692187 MsaT005732.1:CDS
GGCAGTAAATCTATGTCATG+AGG 0.629460 1:-84690947 MsaT005732.1:CDS
GATCCAGTTCATGAACACTC+TGG 0.629849 1:-84689278 MsaT005732.1:CDS
AAGACAGAGAAGAAGAAACC+TGG 0.631466 1:-84692955 MsaT005732.1:CDS
TGACGCTGCAGAAGAAGAAG+GGG 0.632103 1:-84692986 MsaT005732.1:CDS
TTACAGGTCTGGAGAAACCA+AGG 0.633111 1:-84680684 MsaT005732.1:intron
GTATCCTCACTTGAAAGAGG+AGG 0.634520 1:-84692218 MsaT005732.1:CDS
GGAAGCTAAATCGTTCGTCT+CGG 0.635783 1:+84693458 None:intergenic
TGAAACAGGGGATGGTGCTG+AGG 0.636264 1:-84693166 MsaT005732.1:CDS
GCATAAGAAGAGGCTTAAGG+AGG 0.637213 1:-84682888 MsaT005732.1:CDS
AGCGTTTGATAACGGAACAA+AGG 0.637695 1:-84693310 MsaT005732.1:CDS
ACATCATCGGCATTTGGACA+GGG 0.638392 1:+84691204 None:intergenic
GTCCGCCGATGCTACCACTC+CGG 0.638859 1:-84693229 MsaT005732.1:CDS
AGTAAATCACAGGTCTGCAG+AGG 0.639228 1:-84681444 MsaT005732.1:CDS
TGAATCGAAGCATACTGCAT+CGG 0.639909 1:+84689746 None:intergenic
GTGAATGGTCAAGATCTGGT+TGG 0.640659 1:-84691251 MsaT005732.1:CDS
AATCAAGCTTCAAGATGCAG+AGG 0.641093 1:-84691091 MsaT005732.1:CDS
TTGGTGTGAGGAAGAGTGAA+AGG 0.641337 1:-84693122 MsaT005732.1:CDS
GAGATAGAAGCAAAGAGACA+GGG 0.641558 1:-84691923 MsaT005732.1:intron
CTTTGTTGCTGCGAGCTCCG+TGG 0.644754 1:-84691405 MsaT005732.1:CDS
AAACTTCTTATACTGTCCTA+GGG 0.644869 1:-84690968 MsaT005732.1:CDS
GATACGGCGTCGTTGAAGGA+CGG 0.645621 1:-84693549 MsaT005732.1:CDS
TCCTCAAATAAATTAGACGG+AGG 0.648189 1:+84689975 None:intergenic
CAGAGAAGAAGAAACCTGGT+GGG 0.649094 1:-84692951 MsaT005732.1:CDS
TGACTATCGAGCATAAGAAG+AGG 0.650348 1:-84682898 MsaT005732.1:CDS
AAGGAAAATGAAACAGGGGA+TGG 0.652859 1:-84693174 MsaT005732.1:CDS
ATGAACTCGGCACAATGACG+GGG 0.653643 1:+84689945 None:intergenic
TCAATGTCAGAGAAATGACA+AGG 0.658738 1:-84692494 MsaT005732.1:CDS
TGAGTGCTTTCGTTTGACGG+AGG 0.659243 1:-84681483 MsaT005732.1:CDS
CCAGTTCATGAACACTCTGG+TGG 0.660694 1:-84689275 MsaT005732.1:CDS
TGGCAAGGATGATATGAACT+CGG 0.660742 1:+84689932 None:intergenic
TTCCGTCGAAGGTGATGACG+TGG 0.661595 1:-84693439 MsaT005732.1:CDS
AAAAGTGCTGATCATGGATG+GGG 0.662820 1:+84682924 None:intergenic
TTAGGTTGGGTAGCAGCACG+AGG 0.663110 1:+84691279 None:intergenic
TCTGTTCTACAGCCAGACAT+GGG 0.664090 1:-84689849 MsaT005732.1:CDS
GGTTGGCTTGCAGATAGTGA+TGG 0.664390 1:-84691234 MsaT005732.1:CDS
GAGTAAAGGTGATGATGATG+TGG 0.669749 1:-84693280 MsaT005732.1:CDS
ACCACAGGAAATCAAAACCA+AGG 0.673075 1:-84692047 MsaT005732.1:intron
TATACACCATAACACAAGGA+AGG 0.673564 1:-84691016 MsaT005732.1:CDS
GAAAGAGGAGGAAATTGCCG+AGG 0.678490 1:-84692206 MsaT005732.1:CDS
CCTTCTGCAGTCATGCACCA+AGG 0.679106 1:-84681540 MsaT005732.1:intron
GGTGGCGCTGATGATGATGG+GGG 0.679452 1:-84689257 MsaT005732.1:CDS
AATGCACGCCACATGACAAG+CGG 0.682465 1:+84690835 None:intergenic
ACAGAGAAGAAGAAACCTGG+TGG 0.692239 1:-84692952 MsaT005732.1:CDS
TGGCATTGTGATAATCGGGA+TGG 0.694665 1:-84689413 MsaT005732.1:CDS
GGGCGTGATTATCTGCATGG+TGG 0.701277 1:-84691330 MsaT005732.1:CDS
TTGCAGTTACCACAGCACAC+CGG 0.717468 1:+84692178 None:intergenic
CTGCATCGGACATATCACAA+TGG 0.718824 1:+84689760 None:intergenic
CAATGTCAGAGAAATGACAA+GGG 0.723230 1:-84692493 MsaT005732.1:CDS
TGATTGCTTAGATTGGTGTG+AGG 0.724166 1:-84690761 MsaT005732.1:intron
GGAGAAGGAAAATGAAACAG+GGG 0.726313 1:-84693178 MsaT005732.1:CDS
TCAATGGCACTGGAGCAAAG+GGG 0.769506 1:-84690911 MsaT005732.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 84680673 84693655 84680673 ID=MsaG005732
Chr1 mRNA 84680673 84693655 84680673 ID=MsaT005732.1;Parent=MsaG005732
Chr1 exon 84680673 84680700 84680673 ID=MsaT005732.1.exon13;Parent=MsaT005732.1
Chr1 CDS 84680673 84680700 84680673 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Chr1 exon 84680890 84680948 84680890 ID=MsaT005732.1.exon12;Parent=MsaT005732.1
Chr1 CDS 84680890 84680948 84680890 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Chr1 exon 84681433 84681552 84681433 ID=MsaT005732.1.exon11;Parent=MsaT005732.1
Chr1 CDS 84681433 84681552 84681433 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Chr1 exon 84682635 84682720 84682635 ID=MsaT005732.1.exon10;Parent=MsaT005732.1
Chr1 CDS 84682635 84682720 84682635 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Chr1 exon 84682882 84682954 84682882 ID=MsaT005732.1.exon9;Parent=MsaT005732.1
Chr1 CDS 84682882 84682954 84682882 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Chr1 exon 84689136 84689537 84689136 ID=MsaT005732.1.exon8;Parent=MsaT005732.1
Chr1 CDS 84689136 84689537 84689136 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Chr1 exon 84689761 84690090 84689761 ID=MsaT005732.1.exon7;Parent=MsaT005732.1
Chr1 CDS 84689761 84690090 84689761 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Chr1 exon 84690762 84691497 84690762 ID=MsaT005732.1.exon6;Parent=MsaT005732.1
Chr1 CDS 84690762 84691497 84690762 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Chr1 exon 84691573 84691636 84691573 ID=MsaT005732.1.exon5;Parent=MsaT005732.1
Chr1 CDS 84691573 84691636 84691573 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Chr1 exon 84691924 84692062 84691924 ID=MsaT005732.1.exon4;Parent=MsaT005732.1
Chr1 CDS 84691924 84692062 84691924 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Chr1 exon 84692141 84692240 84692141 ID=MsaT005732.1.exon3;Parent=MsaT005732.1
Chr1 CDS 84692141 84692240 84692141 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Chr1 exon 84692430 84692545 84692430 ID=MsaT005732.1.exon2;Parent=MsaT005732.1
Chr1 CDS 84692430 84692545 84692430 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Chr1 exon 84692645 84693655 84692645 ID=MsaT005732.1.exon1;Parent=MsaT005732.1
Chr1 CDS 84692645 84693655 84692645 ID=cds.MsaT005732.1;Parent=MsaT005732.1
Gene Sequence

>MsaT005732.1

ATGGCGGGTGAAGAAAACAACACAGTTACAGAAACGGTGCCGTTAAATGTTGTTGACTTGGCACAAACCGTGAATGTTGTCGCCGATACGGCGTCGTTGAAGGACGGTGAAAGAAGCGATGACGTGGCAGTAGAAGATGAATCTGTTCTCGATCGTGAAAAACAAGCTATTGTCTCCGAGACGAACGATTTAGCTTCCGTCGAAGGTGATGACGTGGCAGCCGGAGATTCTTCTTCCGTTAAAGTGATAAGAAAGTACGGTAGAAGGAAAAAGAAACCGGGAAGAAAAAGTAAGGCTGAAATCGAGAAGGAGAAGATGGAAGAAGCGTTTGATAACGGAACAAAGGAAGGAACGAGTAAAGGTGATGATGATGTGGCAGCCGGAGACTCTGTTTCCGTTCATGCGTCCGCCGATGCTACCACTCCGGCAAAGAAACGCCGTAGAAATAGAAAAACGGAGAAGGAAAATGAAACAGGGGATGGTGCTGAGGTGGAAGGAGGGTTTTCTTCTGTTGGTGTGAGGAAGAGTGAAAGGCCTAGGAAGATTAAATCGTTGAAGGAAGATTACGTTTCAGATTTGGAGGAGGATTTGAAGAAAAAGGGGAAGCGTGGTAAGAAGAAGGTGATAATCGGTGCTTCCGATGAAAATGACGCTGCAGAAGAAGAAGGGGAAAATGTGAAGACAGAGAAGAAGAAACCTGGTGGGAAAAGGAAGGAAATTTTTAGTTCTGGAGATGAAAATGAAGCTGAAAAAGAGGAGGGAAATGTAAAAAAAGAAAAGAAGAAACCTGGTAGAAAAAAGAAAATAAGTTCTAAAGAAAATGATGATGAACAAGAGGGGGGAAATGGTAGAAATGTTAAGATTGAGGAGAATGAATTGATCAGTGAAAAGAATCCGGAGAGTGATGTTTTATCCGATGATAATAAAGGTTATTCATTAAGAACTGATAGAAGAATGAAAGTTAAAAGCAGTGAACAGGCGAAGACAACAATCAATAGATGGGATCCTAAGTGGATTGAAGAGGAGTCCTTAATGTGCCATCAATGTCAGAGAAATGACAAGGGCAGGGTTGTTAGATGCACAAAATGCAAACGAAAAAGATATTGCATACCTTGTTTAAACAATTGGTATCCTCACTTGAAAGAGGAGGAAATTGCCGAGGCATGTCCGGTGTGCTGTGGTAACTGCAATTGCAAAGCATGCTTGCGATCATGTGTACTTATTGAGGAAATCAAAACCAAGGCGAAAACCAACAACGACCATGAGATTGAGCTCTCCAAGTATATGCTGAAACAACTTCTGCCATATTTAAGACGGTTGGATGAGGAGCAAATGGCTGAGAAGGAGATAGAAGCAAAGAGACAGGGGCTCTCCCTTTCTGAGGTAAAAGTAAAAGTTGCAAATTACCCTAAAAAGGAGCGTGTATACTGCGACAACTGCAAAACGTCAATATTTGACTACCACAGAAGCTGTACAAAATGCTCTTTTGACATTTGTCTCCTTTGTTGCTGCGAGCTCCGTGGTGGGAAGCTTCTTGGTGGTGCAGATCCAATCGAGTTCGAATTTATATTCCAAGGGCGTGATTATCTGCATGGTGGAAATGCTAATGAAGTTAAAAAAAATGAACCTCGTGCTGCTACCCAACCTAAGATTCGTGAATGGTCAAGATCTGGTTGGCTTGCAGATAGTGATGGCAGCATTCCCTGTCCAAATGCCGATGATGTTTGTGATCATGGTTTCCTGGAACTGAGAAGCATACTTCCACGAAATTGCATCTCCGAGTTAGTATGTAAAGCAAAAAAACTTGAAGAAACAATCAAGCTTCAAGATGCAGAGGACACATCTAATAGTAGGTGTTTTTGTTCGAAGCCTGTTGTGAATGCATCTGGTATACACCATAACACAAGGAAGGCAGCTTCTCGTGAAGATTCCAGTGAAAACTTCTTATACTGTCCTAGGGCAGTAAATCTATGTCATGAGGATCTAAGACATTTTCAATGGCACTGGAGCAAAGGGGAGCCTATAATTGTCAGCAATGTACTTGAATGTACATCTGGTTTAAGCTGGGAACCGCTTGTCATGTGGCGTGCATTCCGTCAGATAAGCAATACCAAACACAAAACACTTTTGGATGTGAAGGCAATTGATTGCTTAGATTGGTGTGAGGGAGATATTAATGTGCACCAATTCTTTACTGGGTACACAAAGGGTCGCCTGGATTGGCTCAACTGGCCTCAGGTTTTGAAACTGAAAGATTGGCCTCCGTCTAATTTATTTGAGGAAAGTTTACCCCGTCATTGTGCCGAGTTCATATCATCCTTGCCATTTAAAGAATATACAGATCCTTTCAAAGGTGTTCTAAACCTTGCTGTGAAGTTGCCTAAATCTGTTCTACAGCCAGACATGGGCCCAAAAACATATATTGCTTATGGATTTGCTCAGGAGCTTGGGCGGGGTGATTCTGTCACTAAGCTCCATTGTGATATGTCCGATGCAGTAAATGTGTTGACTCATATTGCTGAAGTAAAATTGGAAACTAAAGGAATTGATGCCATTAAGGAACTGAAACTAGCGCACCTTAAGCAAGACAAGAGGGAGTGGCATTGTGATAATCGGGATGGTGAGACCAACATTGACATGCTTGATAATTCATCTTTGATAAATGCTTCAGACGAGCAAAATAACGTTCAAGTCGTGGAAAATGGAAGTGGATTATGTGATGCTAAAGTGGTTGATCCAGTTCATGAACACTCTGGTGGCGCTGATGATGATGGGGGTGATTCTTTAGATGGTGCTCTCTGGGACATTTTTCGGAGAGAGGATGTACCTAAGTTGGAGGAATATTTAAAGAAGCATTTCAGGGAGTTTAGGCATGTCCATTGCTCTCCTTTAAAGCAGGTTATTCACCCCATCCATGATCAGCACTTTTACCTGACTATCGAGCATAAGAAGAGGCTTAAGGAGGAGTATGGTATAGAACCTTGGACATTTTTTCAGAAGCTGGGAGATGCTGTTTTCATTCCAGCTGGTTGTCCGCATCAAGTTCGAAATCTTAAGTCATGCACCAAGGTTGCGCTTGATTTTGTTTCTCCTGAAAATGTTGGTGAGTGCTTTCGTTTGACGGAGGAATTCCGTAAACTTCCAGTAAATCACAGGTCTGCAGAGGACAAATTGGAGGTGAAGAAAATGATTATATATGCTATGCTTGATCTGGTCAAGAATTTGGAGAAAGCAAGGTCTGGAGAAACCAAGGTTCCAGTGTAA
Protein sequence

>MsaT005732.1

MAGEENNTVTETVPLNVVDLAQTVNVVADTASLKDGERSDDVAVEDESVLDREKQAIVSETNDLASVEGDDVAAGDSSSVKVIRKYGRRKKKPGRKSKAEIEKEKMEEAFDNGTKEGTSKGDDDVAAGDSVSVHASADATTPAKKRRRNRKTEKENETGDGAEVEGGFSSVGVRKSERPRKIKSLKEDYVSDLEEDLKKKGKRGKKKVIIGASDENDAAEEEGENVKTEKKKPGGKRKEIFSSGDENEAEKEEGNVKKEKKKPGRKKKISSKENDDEQEGGNGRNVKIEENELISEKNPESDVLSDDNKGYSLRTDRRMKVKSSEQAKTTINRWDPKWIEEESLMCHQCQRNDKGRVVRCTKCKRKRYCIPCLNNWYPHLKEEEIAEACPVCCGNCNCKACLRSCVLIEEIKTKAKTNNDHEIELSKYMLKQLLPYLRRLDEEQMAEKEIEAKRQGLSLSEVKVKVANYPKKERVYCDNCKTSIFDYHRSCTKCSFDICLLCCCELRGGKLLGGADPIEFEFIFQGRDYLHGGNANEVKKNEPRAATQPKIREWSRSGWLADSDGSIPCPNADDVCDHGFLELRSILPRNCISELVCKAKKLEETIKLQDAEDTSNSRCFCSKPVVNASGIHHNTRKAASREDSSENFLYCPRAVNLCHEDLRHFQWHWSKGEPIIVSNVLECTSGLSWEPLVMWRAFRQISNTKHKTLLDVKAIDCLDWCEGDINVHQFFTGYTKGRLDWLNWPQVLKLKDWPPSNLFEESLPRHCAEFISSLPFKEYTDPFKGVLNLAVKLPKSVLQPDMGPKTYIAYGFAQELGRGDSVTKLHCDMSDAVNVLTHIAEVKLETKGIDAIKELKLAHLKQDKREWHCDNRDGETNIDMLDNSSLINASDEQNNVQVVENGSGLCDAKVVDPVHEHSGGADDDGGDSLDGALWDIFRREDVPKLEEYLKKHFREFRHVHCSPLKQVIHPIHDQHFYLTIEHKKRLKEEYGIEPWTFFQKLGDAVFIPAGCPHQVRNLKSCTKVALDFVSPENVGECFRLTEEFRKLPVNHRSAEDKLEVKKMIIYAMLDLVKNLEKARSGETKVPV*