AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005733

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005733 MtrunA17_Chr1g0211691 86.998 946 84 6 1 908 1 945 0.0 1557
MsaG005733 MtrunA17_Chr1g0211711 71.790 1067 191 13 42 1020 7 1051 0.0 1482
MsaG005733 MtrunA17_Chr1g0183311 70.118 850 181 7 234 1020 43 882 0.0 1172
MsaG005733 MtrunA17_Chr5g0426961 69.051 769 201 7 270 1019 84 834 0.0 1058
MsaG005733 MtrunA17_Chr1g0211661 72.727 671 157 6 304 960 207 865 0.0 995
MsaG005733 MtrunA17_Chr1g0211661 80.833 120 23 0 1 120 1 120 1.73e-49 189
MsaG005733 MtrunA17_Chr5g0420361 64.941 676 199 10 385 1029 138 806 0.0 870
MsaG005733 MtrunA17_Chr6g0472441 63.253 664 176 13 385 1013 80 710 0.0 819
MsaG005733 MtrunA17_Chr7g0275771 41.888 943 411 24 184 1025 24 930 0.0 657
MsaG005733 MtrunA17_Chr5g0448301 59.649 570 181 8 379 930 93 631 0.0 633
MsaG005733 MtrunA17_Chr5g0448301 40.741 162 64 8 40 189 2 143 2.67e-11 67.8
MsaG005733 MtrunA17_Chr4g0075261 46.586 747 320 15 311 1017 157 864 0.0 630
MsaG005733 MtrunA17_Chr2g0289121 48.212 699 324 11 322 1017 156 819 0.0 612
MsaG005733 MtrunA17_Chr1g0192011 36.735 735 393 15 322 1021 267 964 6.06e-146 461
MsaG005733 MtrunA17_Chr2g0289031 53.827 405 165 3 615 1017 9 393 1.25e-139 424
MsaG005733 MtrunA17_Chr1g0147021 43.879 531 256 9 316 832 864 1366 4.86e-128 427
MsaG005733 MtrunA17_Chr1g0147021 64.780 159 53 1 873 1028 1551 1709 3.64e-58 219
MsaG005733 MtrunA17_Chr2g0281051 33.987 765 403 16 322 1009 207 946 1.00e-127 412
MsaG005733 MtrunA17_Chr1g0211701 95.690 116 5 0 906 1021 4 119 4.72e-72 236
MsaG005733 MtrunA17_Chr8g0364421 48.828 256 86 5 432 673 5 229 2.60e-66 223
MsaG005733 MtrunA17_Chr7g0231061 59.574 188 69 2 668 855 8 188 3.85e-63 213
MsaG005733 MtrunA17_Chr4g0048531 41.216 296 152 7 543 822 727 1016 1.64e-60 226
MsaG005733 MtrunA17_Chr4g0048531 51.701 147 70 1 872 1018 1132 1277 1.39e-43 172
MsaG005733 MtrunA17_Chr4g0048531 36.585 164 97 3 322 479 260 422 1.97e-26 117
MsaG005733 MtrunA17_Chr8g0361381 55.080 187 80 2 610 796 26 208 6.16e-60 204
MsaG005733 MtrunA17_Chr6g0469821 70.068 147 37 2 1 147 1 140 2.13e-55 188
MsaG005733 MtrunA17_Chr8g0340381 65.476 168 39 3 709 876 36 184 2.50e-55 190
MsaG005733 MtrunA17_Chr2g0289091 72.973 111 30 0 872 982 310 420 2.71e-49 181
MsaG005733 MtrunA17_Chr2g0289091 59.441 143 57 1 687 829 22 163 2.49e-47 175
MsaG005733 MtrunA17_Chr8g0361391 64.545 110 38 1 851 960 29 137 1.49e-41 149
MsaG005733 MtrunA17_Chr2g0289041 39.070 215 113 7 344 552 2 204 9.12e-39 143
MsaG005733 MtrunA17_Chr8g0364411 57.143 112 48 0 322 433 33 144 4.35e-36 134
MsaG005733 MtrunA17_Chr1g0211681 70.968 93 26 1 55 147 1 92 1.90e-32 121
MsaG005733 MtrunA17_Chr1g0211671 76.190 63 15 0 959 1021 15 77 1.34e-27 108
MsaG005733 MtrunA17_Chr3g0119761 66.234 77 26 0 782 858 55 131 4.86e-25 101
MsaG005733 MtrunA17_Chr2g0289101 39.200 125 61 6 454 572 8 123 6.27e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005733 AT1G11950.1 49.930 711 322 10 318 1021 189 872 0.0 691
MsaG005733 AT1G11950.3 49.930 711 322 10 318 1021 189 872 0.0 691
MsaG005733 AT1G62310.1 49.785 699 315 12 321 1006 208 883 0.0 674
MsaG005733 AT1G11950.2 48.750 640 294 11 318 950 189 801 0.0 593
MsaG005733 AT4G00990.1 43.194 764 350 14 322 1016 80 828 0.0 587
MsaG005733 AT3G07610.1 57.582 488 173 6 535 1009 417 883 0.0 562
MsaG005733 AT3G07610.1 39.556 225 133 2 292 516 127 348 1.70e-46 181
MsaG005733 AT3G07610.3 54.832 507 179 7 535 1009 417 905 3.84e-175 540
MsaG005733 AT3G07610.3 39.556 225 133 2 292 516 127 348 2.23e-46 181
MsaG005733 AT4G00990.3 42.172 709 326 14 322 961 80 773 2.79e-170 518
MsaG005733 AT4G00990.2 42.351 706 323 14 322 958 80 770 2.99e-170 518
MsaG005733 AT3G07610.2 44.077 574 248 8 292 802 127 690 4.79e-149 462
MsaG005733 AT1G09060.2 33.882 729 429 17 315 1021 197 894 1.59e-120 392
MsaG005733 AT1G09060.1 33.882 729 429 17 315 1021 197 894 1.59e-120 392
MsaG005733 AT1G09060.4 33.882 729 429 17 315 1021 211 908 3.92e-120 391
MsaG005733 AT1G09060.3 33.882 729 429 17 315 1021 211 908 3.92e-120 391
MsaG005733 AT4G21430.1 35.360 444 246 6 601 1016 481 911 4.62e-82 286
MsaG005733 AT4G21430.1 29.747 158 108 3 316 471 221 377 1.35e-12 72.8

Find 228 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATAAAGGTTATTCTTTA+AGG 0.076090 1:-84723949 MsaT005733.1:CDS
GGCCTCCTTCTAATTTATTT+AGG 0.135585 1:-84720733 MsaT005733.1:CDS
TGTCTTGCTCAAGGTGCTTT+TGG 0.146606 1:+84720004 None:intergenic
TTGCTCGTCTGAAGCATTTA+TGG 0.202791 1:+84719912 None:intergenic
ATTGATCAGTGAAAAGAATT+TGG 0.212830 1:-84723993 MsaT005733.1:CDS
GCAAAATAGTGTTCGAGTTT+TGG 0.215510 1:-84719893 MsaT005733.1:CDS
TCATCCAATCGTCTTAAATA+TGG 0.224411 1:+84723043 None:intergenic
GTCGCCCAGATTGGCTTAAT+TGG 0.224414 1:-84720781 MsaT005733.1:CDS
TTTAAATATTCCTCCAATTC+AGG 0.228132 1:+84719811 None:intergenic
GCCTCCTTCTAATTTATTTA+GGG 0.243943 1:-84720732 MsaT005733.1:CDS
CTTCGTTGTGGGAAGCTTCT+AGG 0.247218 1:-84722497 MsaT005733.1:CDS
GCTTGATGTGGTTAACAAAT+TGG 0.261966 1:-84716017 MsaT005733.1:CDS
AATGTGCACCAATTCTTTAC+TGG 0.265399 1:-84720815 MsaT005733.1:CDS
CCACAATCATCATCGGCTTT+TGG 0.278197 1:+84722305 None:intergenic
CTGACATTGATGGCACATTA+AGG 0.293064 1:+84723714 None:intergenic
AGTACATTGCTGATAATTAC+AGG 0.293307 1:+84721994 None:intergenic
GGATCTGTATATTCTTTATA+CGG 0.299317 1:+84720668 None:intergenic
TACCAAGCACAAAACACTTT+TGG 0.300838 1:-84721905 MsaT005733.1:CDS
ATTCTTTAGATGGTGCTCTC+TGG 0.323154 1:-84719858 MsaT005733.1:CDS
GCATACCTTGTTTAAACAAT+TGG 0.324245 1:-84723636 MsaT005733.1:intron
AGAAGCATTTCAGAGAGTTT+AGG 0.334304 1:-84719789 MsaT005733.1:CDS
TGTCATTTCTCTGACATTGA+TGG 0.335478 1:+84723704 None:intergenic
GGAAGATTACGTGTCAGATT+TGG 0.338618 1:-84724233 MsaT005733.1:CDS
TTGGTGGACCTGTGGTTTAT+CGG 0.340425 1:+84716980 None:intergenic
CAAGGCCGTGATTATCTTCA+TGG 0.341964 1:-84722440 MsaT005733.1:CDS
AGAAGAAACCTGGTAGAAAA+AGG 0.348376 1:-84724099 MsaT005733.1:CDS
TGATCAGGAATGGGTTCATC+TGG 0.355725 1:+84724724 None:intergenic
AATAGGGGTGATTCTTTAGA+TGG 0.356154 1:-84719868 MsaT005733.1:CDS
ATATCAGTTGCATTCCTAAC+AGG 0.357202 1:+84722144 None:intergenic
ACTAACTCAGAGATGCAATT+TGG 0.359225 1:+84722248 None:intergenic
CTCGAACTCATGGTTGTTGT+TGG 0.375822 1:+84723096 None:intergenic
TTTGTGAGTGGTCAAGATTC+GGG 0.378563 1:-84722361 MsaT005733.1:CDS
TTGCAAACTTCCTCTCCCTT+TGG 0.380018 1:-84715976 MsaT005733.1:CDS
AGGAAACTTAAGGAGGAGTA+TGG 0.381395 1:-84717684 MsaT005733.1:intron
CTTTCCCTAAATAAATTAGA+AGG 0.387657 1:+84720728 None:intergenic
ATGACGTAAATTTAAAGCTC+TGG 0.387898 1:+84722059 None:intergenic
CGCGGAAACGGTACCGTTGA+AGG 0.390300 1:-84724665 MsaT005733.1:CDS
AAGAGGGTGAGGAAAAGAAT+TGG 0.395670 1:-84724339 MsaT005733.1:CDS
TGAGGCCAATTAAGCCAATC+TGG 0.400800 1:+84720776 None:intergenic
ATCTGACTCGAGAGCATAAG+AGG 0.403353 1:-84717704 MsaT005733.1:CDS
GTTCTTGTTTGAAGCCTGTT+AGG 0.405975 1:-84722158 MsaT005733.1:CDS
ATGCTCTCGAGTCAGATAAA+AGG 0.409436 1:+84717710 None:intergenic
TCTGACTTGGTGAGGACAAC+CGG 0.410174 1:+84717491 None:intergenic
AAGATAAACTCAAACTCAAT+TGG 0.413084 1:+84722464 None:intergenic
TTCAATGGCACTGGAGCAAA+GGG 0.413215 1:-84722020 MsaT005733.1:CDS
CTTCCGGTTGTGTGAGAAAG+AGG 0.414317 1:-84724285 MsaT005733.1:CDS
TTCTTTAGATGGTGCTCTCT+GGG 0.419089 1:-84719857 MsaT005733.1:CDS
GAGAGAGGATGTACCTGAAT+TGG 0.421371 1:-84719824 MsaT005733.1:CDS
TATGGATTTGCTCAGGAGCT+TGG 0.422137 1:-84720563 MsaT005733.1:CDS
TTCTTTACTGGGTACACAAA+TGG 0.422160 1:-84720803 MsaT005733.1:CDS
AATGTACTTGAATGTACAAC+TGG 0.424187 1:-84721979 MsaT005733.1:CDS
ATTTGTGAGTGGTCAAGATT+CGG 0.429286 1:-84722362 MsaT005733.1:CDS
TCTTCCATATTTAAGACGAT+TGG 0.429788 1:-84723047 MsaT005733.1:CDS
TTGTGTACCCAGTAAAGAAT+TGG 0.434568 1:+84720807 None:intergenic
AGAGGGTGAGGAAAAGAATT+GGG 0.444632 1:-84724338 MsaT005733.1:CDS
CAAAGTCACACTCACCGTTC+CGG 0.446960 1:-84724749 MsaT005733.1:CDS
AAGTTCTTTCAGCATATACT+TGG 0.448229 1:+84723069 None:intergenic
AATGTACAACTGGTTTAAGC+TGG 0.448278 1:-84721969 MsaT005733.1:CDS
TGCCAATGTTGGCGCGGAAA+CGG 0.450167 1:-84724677 MsaT005733.1:CDS
AGCTGATGACATGGCAGCTA+AGG 0.454453 1:-84724464 MsaT005733.1:CDS
TTGCCTCTTTCTCACACAAC+CGG 0.456045 1:+84724282 None:intergenic
GAAGAAGAAGGTCATAATTG+AGG 0.456146 1:-84724176 MsaT005733.1:CDS
AGAGCATAAGAGGAAACTTA+AGG 0.461052 1:-84717694 MsaT005733.1:CDS
CTGCTTTAAAGGAGAGCAAT+GGG 0.464779 1:+84719762 None:intergenic
TGAGAAAGAGGCAAAGGCCT+AGG 0.465093 1:-84724273 MsaT005733.1:CDS
AGATTGGCTTAATTGGCCTC+AGG 0.465478 1:-84720774 MsaT005733.1:CDS
GAGATAGAAGCAAAGAGAAA+AGG 0.470722 1:-84723001 MsaT005733.1:intron
CATGAAGATAATCACGGCCT+TGG 0.473194 1:+84722441 None:intergenic
TGCTTGTAGGAATCGAGCCT+TGG 0.475187 1:-84717554 MsaT005733.1:intron
ACACAAATGGTCGCCCAGAT+TGG 0.475261 1:-84720790 MsaT005733.1:CDS
GACAGTTTATAGTTCGGAAG+AGG 0.480891 1:-84724356 MsaT005733.1:CDS
TGACGTAAATTTAAAGCTCT+GGG 0.482957 1:+84722060 None:intergenic
GTCAGAGAAATGACAAGGGC+AGG 0.483009 1:-84723696 MsaT005733.1:CDS
ACCACTCACAAATCTCAGGC+TGG 0.483334 1:+84722372 None:intergenic
TATTGCTTATGGATTTGCTC+AGG 0.486399 1:-84720570 MsaT005733.1:CDS
AGGCAATTGATTGCTTAGAT+TGG 0.486412 1:-84721876 MsaT005733.1:CDS
TGTGGTTTATCGGAAGCTTA+CGG 0.487799 1:+84716990 None:intergenic
GTTTCTCGACGATCATGATC+AGG 0.488002 1:+84724709 None:intergenic
ACGATTGGATGAAGAACAAA+TGG 0.491089 1:-84723032 MsaT005733.1:CDS
ATGTGCACCAATTCTTTACT+GGG 0.492244 1:-84720814 MsaT005733.1:CDS
TCGACGATCATGATCAGGAA+TGG 0.495893 1:+84724714 None:intergenic
AGCTTTAAATTTACGTCATG+AGG 0.498076 1:-84722055 MsaT005733.1:CDS
CTCGAGTCAGATAAAAGGTC+TGG 0.498993 1:+84717715 None:intergenic
TAGGAAGATTAAATCGTTGA+AGG 0.499461 1:-84724254 MsaT005733.1:CDS
TTACCTTTAGATTTCTGACT+TGG 0.503046 1:+84717478 None:intergenic
CGACGATCATGATCAGGAAT+GGG 0.504764 1:+84724715 None:intergenic
GACCTCCAACCTGTCCTTGG+TGG 0.508939 1:+84716964 None:intergenic
TTCAACGATTTAATCTTCCT+AGG 0.509264 1:+84724256 None:intergenic
GCTTGGTATTTCTTATCTGA+CGG 0.510840 1:+84721920 None:intergenic
ACCTGTCCTTGGTGGACCTG+TGG 0.512572 1:+84716972 None:intergenic
CTTGACCACTCACAAATCTC+AGG 0.514084 1:+84722368 None:intergenic
ACAGACCTCCAACCTGTCCT+TGG 0.514908 1:+84716961 None:intergenic
TTTCAATGGCACTGGAGCAA+AGG 0.515097 1:-84722021 MsaT005733.1:CDS
AGATTACGTGTCAGATTTGG+AGG 0.516385 1:-84724230 MsaT005733.1:CDS
GTTGTTGTGAGCTTCGTTGT+GGG 0.519321 1:-84722508 MsaT005733.1:CDS
GCTCCCTCTTGTCTTGCTCA+AGG 0.519343 1:+84719995 None:intergenic
TACCGTTTCCGCGCCAACAT+TGG 0.523494 1:+84724675 None:intergenic
TACGGAATTCCTCAGTCAAA+CGG 0.525984 1:+84717008 None:intergenic
GAGTTTGAGTTTATCTTCCA+AGG 0.526474 1:-84722458 MsaT005733.1:CDS
AAGACAGAGCAGAAGAAACC+TGG 0.528230 1:-84724109 MsaT005733.1:CDS
TTATAGTTCGGAAGAGGGTG+AGG 0.529955 1:-84724350 MsaT005733.1:CDS
TTGTAGTCATGCATCAAGGT+TGG 0.532619 1:-84717070 MsaT005733.1:intron
GAAGTATGCTTCTCAGTTCG+AGG 0.532683 1:+84722273 None:intergenic
TATCGGTGTTAGAGCTGAAA+CGG 0.532983 1:-84724623 MsaT005733.1:CDS
GGAATGGGTTCATCTGGGTC+CGG 0.533236 1:+84724730 None:intergenic
GAAGGTGCTGACGTGGAAGA+AGG 0.533445 1:-84724316 MsaT005733.1:CDS
CTTAATGGCAGCAATGCCCT+CGG 0.540894 1:+84720035 None:intergenic
TGTATATTCTTTATACGGCA+AGG 0.541667 1:+84720673 None:intergenic
TGCCATGTCATCAGCTTCAA+CGG 0.544773 1:+84724471 None:intergenic
CCATTGCTCTCCTTTAAAGC+AGG 0.546741 1:-84719761 MsaT005733.1:intron
AACTCAGAGATGCAATTTGG+TGG 0.548310 1:+84722251 None:intergenic
TATATCAGATTCATAACCAA+AGG 0.550769 1:+84715960 None:intergenic
TGAGATGCACAAAATGCAAA+CGG 0.552047 1:-84723669 MsaT005733.1:CDS
ATAAAAGGTCTGGTCGTGGA+TGG 0.552092 1:+84717725 None:intergenic
CCTGCTTTAAAGGAGAGCAA+TGG 0.552913 1:+84719761 None:intergenic
GCCGATGATGATTGTGGTCA+TGG 0.553086 1:-84722299 MsaT005733.1:CDS
TTGTTGTCGACGGACGATGA+AGG 0.553268 1:-84724496 MsaT005733.1:CDS
ACTTGGAGAGCTCGAACTCA+TGG 0.555503 1:+84723086 None:intergenic
TCACCAAGTCAGAAATCTAA+AGG 0.555814 1:-84717481 MsaT005733.1:intron
AGAGGATGTACCTGAATTGG+AGG 0.558183 1:-84719821 MsaT005733.1:CDS
AGGTTAAGAATGCCTTTGAA+AGG 0.562396 1:+84720647 None:intergenic
ACCACAGGTCCACCAAGGAC+AGG 0.565099 1:-84716973 MsaT005733.1:CDS
ATTAAAGAGAAGAAGAGAGC+TGG 0.566428 1:-84724394 MsaT005733.1:CDS
GGAATACAAGAAGTTGTCAC+TGG 0.567927 1:+84722083 None:intergenic
TCAGGCTGGGCAGCAGAATG+AGG 0.568737 1:+84722386 None:intergenic
GAATGATGAAGCTGAACAAG+AGG 0.569424 1:-84724143 MsaT005733.1:CDS
TGTTGTTGTGAGCTTCGTTG+TGG 0.569590 1:-84722509 MsaT005733.1:CDS
GCACCTTGAGCAAGACAAGA+GGG 0.570458 1:-84719998 MsaT005733.1:CDS
ATGTACAACTGGTTTAAGCT+GGG 0.570533 1:-84721968 MsaT005733.1:CDS
TGAAGCTGAACAAGAGGAGG+AGG 0.572752 1:-84724137 MsaT005733.1:CDS
GTTGTCACTGGAATCTTCAC+GGG 0.575662 1:+84722095 None:intergenic
GAATATACAGATCCTTTCAA+AGG 0.575964 1:-84720659 MsaT005733.1:CDS
GCGGAAACGGTACCGTTGAA+GGG 0.579178 1:-84724664 MsaT005733.1:CDS
TAAAAGGTCTGGTCGTGGAT+GGG 0.579331 1:+84717726 None:intergenic
AGTTGTCACTGGAATCTTCA+CGG 0.579890 1:+84722094 None:intergenic
TTGTACCAATTGTTTAAACA+AGG 0.581322 1:+84723631 None:intergenic
ACAGTTTATAGTTCGGAAGA+GGG 0.583088 1:-84724355 MsaT005733.1:CDS
AAGGTATCCTCACTTGAAAG+AGG 0.584254 1:-84723302 MsaT005733.1:CDS
ATATCAGATTCATAACCAAA+GGG 0.585135 1:+84715961 None:intergenic
GATGACGTGGCACAAGCTAT+CGG 0.585670 1:-84724640 MsaT005733.1:CDS
TCTTTCAATTTCAAAACCTG+AGG 0.586296 1:+84720758 None:intergenic
CTACATGGTGATAATCGAGA+TGG 0.586913 1:-84719973 MsaT005733.1:CDS
TCAGATAAAAGGTCTGGTCG+TGG 0.587061 1:+84717721 None:intergenic
ATTTGAAATTTCAATGGCGG+AGG 0.588198 1:-84724809 None:intergenic
ACTTGGTGAGGACAACCGGC+AGG 0.588247 1:+84717495 None:intergenic
CCACTCACAAATCTCAGGCT+GGG 0.588333 1:+84722373 None:intergenic
TTTAGATTTCTGACTTGGTG+AGG 0.588372 1:+84717483 None:intergenic
GAGGCCAATTAAGCCAATCT+GGG 0.589315 1:+84720777 None:intergenic
TTTGTCTCACCTGAAAATGT+TGG 0.589435 1:-84717040 MsaT005733.1:CDS
CAAGACAAGAGGGAGCTACA+TGG 0.591607 1:-84719988 MsaT005733.1:CDS
TCCCTAAATAAATTAGAAGG+AGG 0.592028 1:+84720731 None:intergenic
CAATTAACAGTGGATTGAAG+AGG 0.593067 1:-84723740 MsaT005733.1:intron
AGCTTCTGAATAAAAGTCCA+AGG 0.593398 1:+84717537 None:intergenic
GCTGGGAACCGCTTGTCATG+TGG 0.594440 1:-84721951 MsaT005733.1:CDS
TTTGCTCAGGAGCTTGGACG+TGG 0.595460 1:-84720557 MsaT005733.1:CDS
GTCCACCAAGGACAGGTTGG+AGG 0.596001 1:-84716966 MsaT005733.1:intron
AAATGTTCTAAAGCCAGACA+TGG 0.598652 1:-84720606 MsaT005733.1:CDS
ACACAACCTACTGCCAATGT+TGG 0.598702 1:-84724688 MsaT005733.1:CDS
TGAAGAACAAATGGTCGAGA+AGG 0.598904 1:-84723023 MsaT005733.1:CDS
TCCGCGCCAACATTGGCAGT+AGG 0.599431 1:+84724682 None:intergenic
AACGGTGGTGTTGTTGTCGA+CGG 0.600381 1:-84724506 MsaT005733.1:CDS
GAATTGGGAAGGTGCTGACG+TGG 0.602384 1:-84724323 MsaT005733.1:CDS
ACCTACTGCCAATGTTGGCG+CGG 0.602878 1:-84724683 MsaT005733.1:CDS
CAGGTCCACCAAGGACAGGT+TGG 0.604718 1:-84716969 MsaT005733.1:CDS
CAATGCTGAACAAGAGTGTG+AGG 0.604968 1:-84724428 MsaT005733.1:CDS
GAAACGTGGTAGGAAGAAGA+AGG 0.606573 1:-84724188 MsaT005733.1:CDS
GGTGAGGAAAAGAATTGGGA+AGG 0.609015 1:-84724334 MsaT005733.1:CDS
GGTGCTGACGTGGAAGAAGG+AGG 0.610124 1:-84724313 MsaT005733.1:CDS
GATCAGGAATGGGTTCATCT+GGG 0.610176 1:+84724725 None:intergenic
AGATTCATAACCAAAGGGAG+AGG 0.611184 1:+84715966 None:intergenic
ACCATGACCACAATCATCAT+CGG 0.612092 1:+84722298 None:intergenic
ACGTAAATTTAAAGCTCTGG+GGG 0.612630 1:+84722062 None:intergenic
TTACGTGTCAGATTTGGAGG+AGG 0.613999 1:-84724227 MsaT005733.1:CDS
AAATGACGAAGATGAACAAG+AGG 0.614355 1:-84724053 MsaT005733.1:CDS
ACTTCCTCCTCTTTCAAGTG+AGG 0.615486 1:+84723295 None:intergenic
GAAGTGAAATTGAAATCCGA+GGG 0.618068 1:-84720051 MsaT005733.1:CDS
AGCACCTTGAGCAAGACAAG+AGG 0.618569 1:-84719999 MsaT005733.1:CDS
GGGTTCATCTGGGTCCGGAA+CGG 0.619249 1:+84724735 None:intergenic
TGATGAAGCTGAACAAGAGG+AGG 0.621390 1:-84724140 MsaT005733.1:CDS
TTCCGTTGAAGCTGATGACA+TGG 0.621539 1:-84724473 MsaT005733.1:CDS
TTCATGGTGGAAAAGAAGAG+AGG 0.621997 1:-84722424 MsaT005733.1:CDS
AATTAGGCTTCAAGATGCAG+AGG 0.623081 1:-84722199 MsaT005733.1:intron
TTGCAATTACCACAGCACAC+TGG 0.624403 1:+84723259 None:intergenic
TCAGAGAAATGACAAGGGCA+GGG 0.624776 1:-84723695 MsaT005733.1:CDS
TTTCAGGCAACTTCACAGCA+AGG 0.625295 1:+84720627 None:intergenic
TCATGGTGGAAAAGAAGAGA+GGG 0.625856 1:-84722423 MsaT005733.1:CDS
GTTGTGTGAGAAAGAGGCAA+AGG 0.627339 1:-84724279 MsaT005733.1:CDS
AATAAACAAACGGAATCCAA+AGG 0.628262 1:-84723879 MsaT005733.1:intron
GCGTTGTTGAATAACGATGA+AGG 0.630307 1:-84724601 MsaT005733.1:CDS
TTCCACCATGAAGATAATCA+CGG 0.630533 1:+84722435 None:intergenic
TTAACAAATTGGAGAAAACA+AGG 0.631124 1:-84716006 MsaT005733.1:CDS
GCCACGTCATCACCCTTCAA+CGG 0.632095 1:+84724652 None:intergenic
GTAAGCTTCCGATAAACCAC+AGG 0.632121 1:-84716988 MsaT005733.1:CDS
CCAAAAGCCGATGATGATTG+TGG 0.633999 1:-84722305 MsaT005733.1:CDS
GTGCTGACGTGGAAGAAGGA+GGG 0.635869 1:-84724312 MsaT005733.1:CDS
TATACACAATAACACAAGGA+AGG 0.636388 1:-84722124 MsaT005733.1:CDS
TATATATGCTATGCTTGATG+TGG 0.638461 1:-84716029 MsaT005733.1:CDS
GAAAGAGGAGGAAGTTGCTG+AGG 0.638538 1:-84723287 MsaT005733.1:CDS
TGAAGTGAAATTGAAATCCG+AGG 0.639908 1:-84720052 MsaT005733.1:CDS
GGGTGGCATGCAGATAGTGA+TGG 0.640615 1:-84722341 MsaT005733.1:CDS
GTATCCTCACTTGAAAGAGG+AGG 0.641340 1:-84723299 MsaT005733.1:CDS
ACCGTTGAAGGGTGATGACG+TGG 0.641508 1:-84724653 MsaT005733.1:CDS
TGACGAAGATGAACAAGAGG+GGG 0.641516 1:-84724050 MsaT005733.1:CDS
CTGATATACACAATAACACA+AGG 0.643123 1:-84722128 MsaT005733.1:CDS
TCACTGTATATGTGACTGTG+TGG 0.644617 1:+84724776 None:intergenic
GAGGCATGTCCAGTGTGCTG+TGG 0.646992 1:-84723268 MsaT005733.1:CDS
GACGTAAATTTAAAGCTCTG+GGG 0.647211 1:+84722061 None:intergenic
CCACTGTAATATGTCTGATG+CGG 0.649776 1:-84720516 MsaT005733.1:intron
CGTTGTGGGAAGCTTCTAGG+TGG 0.657294 1:-84722494 MsaT005733.1:CDS
ATTATCAAACATGTCAACGT+TGG 0.657373 1:+84719945 None:intergenic
AATGTTCTAAAGCCAGACAT+GGG 0.657555 1:-84720605 MsaT005733.1:CDS
TCAATGTCAGAGAAATGACA+AGG 0.658738 1:-84723701 MsaT005733.1:CDS
AATGACGAAGATGAACAAGA+GGG 0.659397 1:-84724052 MsaT005733.1:CDS
GCATAAGAGGAAACTTAAGG+AGG 0.660443 1:-84717691 MsaT005733.1:CDS
GTGAGTGGTCAAGATTCGGG+TGG 0.671679 1:-84722358 MsaT005733.1:CDS
GGCCGTGATTATCTTCATGG+TGG 0.676321 1:-84722437 MsaT005733.1:CDS
CCCAGCCTGAGATTTGTGAG+TGG 0.676512 1:-84722373 MsaT005733.1:CDS
AATGCACGCCACATGACAAG+CGG 0.682465 1:+84721943 None:intergenic
TGAGTGCTTCCGTTTGACTG+AGG 0.694932 1:-84717017 MsaT005733.1:CDS
ATGACGAAGATGAACAAGAG+GGG 0.700639 1:-84724051 MsaT005733.1:CDS
TGATTGCTTAGATTGGTGTG+AGG 0.703268 1:-84721869 MsaT005733.1:intron
ATGAACTCAGCACAATGACG+AGG 0.704061 1:+84720701 None:intergenic
GATAAACCACAGGTCCACCA+AGG 0.718071 1:-84716978 MsaT005733.1:CDS
CAATGTCAGAGAAATGACAA+GGG 0.723230 1:-84723700 MsaT005733.1:CDS
CGATGAAGGTGATGACGTGG+TGG 0.737529 1:-84724587 MsaT005733.1:CDS
CCGCATCAGACATATTACAG+TGG 0.743850 1:+84720516 None:intergenic
TAACGATGAAGGTGATGACG+TGG 0.757821 1:-84724590 MsaT005733.1:CDS
TCAATGGCACTGGAGCAAAG+GGG 0.769506 1:-84722019 MsaT005733.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 84715970 84724818 84715970 ID=MsaG005733
Chr1 mRNA 84715970 84724818 84715970 ID=MsaT005733.1;Parent=MsaG005733
Chr1 exon 84715970 84716065 84715970 ID=MsaT005733.1.exon13;Parent=MsaT005733.1
Chr1 CDS 84715970 84716065 84715970 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Chr1 exon 84716967 84717086 84716967 ID=MsaT005733.1.exon12;Parent=MsaT005733.1
Chr1 CDS 84716967 84717086 84716967 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Chr1 exon 84717482 84717567 84717482 ID=MsaT005733.1.exon11;Parent=MsaT005733.1
Chr1 CDS 84717482 84717567 84717482 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Chr1 exon 84717685 84717757 84717685 ID=MsaT005733.1.exon10;Parent=MsaT005733.1
Chr1 CDS 84717685 84717757 84717685 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Chr1 exon 84719762 84720097 84719762 ID=MsaT005733.1.exon9;Parent=MsaT005733.1
Chr1 CDS 84719762 84720097 84719762 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Chr1 exon 84720517 84720846 84720517 ID=MsaT005733.1.exon8;Parent=MsaT005733.1
Chr1 CDS 84720517 84720846 84720517 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Chr1 exon 84721870 84722207 84721870 ID=MsaT005733.1.exon7;Parent=MsaT005733.1
Chr1 CDS 84721870 84722207 84721870 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Chr1 exon 84722246 84722604 84722246 ID=MsaT005733.1.exon6;Parent=MsaT005733.1
Chr1 CDS 84722246 84722604 84722246 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Chr1 exon 84722694 84722757 84722694 ID=MsaT005733.1.exon5;Parent=MsaT005733.1
Chr1 CDS 84722694 84722757 84722694 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Chr1 exon 84723002 84723140 84723002 ID=MsaT005733.1.exon4;Parent=MsaT005733.1
Chr1 CDS 84723002 84723140 84723002 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Chr1 exon 84723222 84723321 84723222 ID=MsaT005733.1.exon3;Parent=MsaT005733.1
Chr1 CDS 84723222 84723321 84723222 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Chr1 exon 84723637 84723752 84723637 ID=MsaT005733.1.exon2;Parent=MsaT005733.1
Chr1 CDS 84723637 84723752 84723637 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Chr1 exon 84723880 84724818 84723880 ID=MsaT005733.1.exon1;Parent=MsaT005733.1
Chr1 CDS 84723880 84724818 84723880 ID=cds.MsaT005733.1;Parent=MsaT005733.1
Gene Sequence

>MsaT005733.1

ATGGCGGAGGAAGAGAACAACCACACAGTCACATATACAGTGAAAATCAAAGTCACACTCACCGTTCCGGACCCAGATGAACCCATTCCTGATCATGATCGTCGAGAAACACAACCTACTGCCAATGTTGGCGCGGAAACGGTACCGTTGAAGGGTGATGACGTGGCACAAGCTATCGGTGTTAGAGCTGAAACGGCGTTGTTGAATAACGATGAAGGTGATGACGTGGTGGAAGCAGCTGAAGAATCTATTCCTGATCGAGAAAAACAAGATATAGATGTTGGCGCAAAAACGGTGGTGTTGTTGTCGACGGACGATGAAGGTTCCGTTGAAGCTGATGACATGGCAGCTAAGGATTCTGATGAAAACAATGCTGAACAAGAGTGTGAGGAAAAAAAAGTCATTAAAGAGAAGAAGAGAGCTGGTAGAAAAAGAAAAAAGACAGTTTATAGTTCGGAAGAGGGTGAGGAAAAGAATTGGGAAGGTGCTGACGTGGAAGAAGGAGGGTTTTCTTCCGGTTGTGTGAGAAAGAGGCAAAGGCCTAGGAAGATTAAATCGTTGAAGGAAGATTACGTGTCAGATTTGGAGGAGGATATGAAGAAAAAGTGGAAACGTGGTAGGAAGAAGAAGGTCATAATTGAGGTTTCTGATGAGAATGATGAAGCTGAACAAGAGGAGGAGGATGTGAAGACAGAGCAGAAGAAACCTGGTAGAAAAAGGAAAGTAGGTTATGGTTCTGAAGAAAATGACGAAGATGAACAAGAGGGGGAAAATGGTAAAAATTTTAAGATTGTTGAGAATGAATTGATCAGTGAAAAGAATTTGGAGAGTGATGTTTTATCAGATGATAATAAAGGTTATTCTTTAAGGACTGATAGAAAAGTGAAAGTTAAAAGCATTGAACAGCCGAAGCAAAAAATAAACAAACGGAATCCAAAGTGGATTGAAGAGGAGTCCTTAATGTGCCATCAATGTCAGAGAAATGACAAGGGCAGGGTTGTGAGATGCACAAAATGCAAACGGAAAAGATTTTGCATACCTTGTTTAAACAATTGGTATCCTCACTTGAAAGAGGAGGAAGTTGCTGAGGCATGTCCAGTGTGCTGTGGTAATTGCAATTGCAAAGCATGCTTGCGATCATGTGCACTTATCAAAGAAATTAAAATGAAGACAGAAACCAACAACAACCATGAGTTCGAGCTCTCCAAGTATATGCTGAAAGAACTTCTTCCATATTTAAGACGATTGGATGAAGAACAAATGGTCGAGAAGGAGATAGAAGCAAAGAGAAAAGGGCTCTCACATTCTAAGCTAAAAGTAAAAGCCGCAGATTACCCTAAAAATAAGCGTGTGTACTGTGACAACTGCAAAACGTCAATATTTGACTACCACAGAAGCTGTACAAAATGCTCTTTTAACATTTGTCTTCTTTGTTGTTGTGAGCTTCGTTGTGGGAAGCTTCTAGGTGGTACAGATCCAATTGAGTTTGAGTTTATCTTCCAAGGCCGTGATTATCTTCATGGTGGAAAAGAAGAGAGGGTAAAAAAAAATAAACCTCATTCTGCTGCCCAGCCTGAGATTTGTGAGTGGTCAAGATTCGGGTGGCATGCAGATAGTGATGGTAGCATTCCCTGTCCAAAAGCCGATGATGATTGTGGTCATGGTTTCCTCGAACTGAGAAGCATACTTCCACCAAATTGCATCTCTGAGTTAGTATATGCAGAGGAGACATTTGATAGTATGTGTTCTTGTTTGAAGCCTGTTAGGAATGCAACTGATATACACAATAACACAAGGAAGGCAGCTTCCCGTGAAGATTCCAGTGACAACTTCTTGTATTCCCCCAGAGCTTTAAATTTACGTCATGAGGATTTAAGACATTTTCAATGGCACTGGAGCAAAGGGGAGCCTGTAATTATCAGCAATGTACTTGAATGTACAACTGGTTTAAGCTGGGAACCGCTTGTCATGTGGCGTGCATTCCGTCAGATAAGAAATACCAAGCACAAAACACTTTTGGATGTGGAGGCAATTGATTGCTTAGATTGGTGTGAGGAAAATATTAATGTGCACCAATTCTTTACTGGGTACACAAATGGTCGCCCAGATTGGCTTAATTGGCCTCAGGTTTTGAAATTGAAAGATTGGCCTCCTTCTAATTTATTTAGGGAAAGTTTGCCTCGTCATTGTGCTGAGTTCATATCTTCCTTGCCGTATAAAGAATATACAGATCCTTTCAAAGGCATTCTTAACCTTGCTGTGAAGTTGCCTGAAAATGTTCTAAAGCCAGACATGGGCCCAAAAACATATATTGCTTATGGATTTGCTCAGGAGCTTGGACGTGGTGATTCTGTGACTAAGCTCCACTGTAATATGTCTGATGCGGTAAATGTGTTGACTCATATTGCTGAAGTGAAATTGAAATCCGAGGGCATTGCTGCCATTAAGAAACTGACCCAAAAGCACCTTGAGCAAGACAAGAGGGAGCTACATGGTGATAATCGAGATGGAGAGACCAACGTTGACATGTTTGATAATTCATCTTCTTCCATAAATGCTTCAGACGAGCAAAATAGTGTTCGAGTTTTGGAAAATAGGGGTGATTCTTTAGATGGTGCTCTCTGGGATATTTTTCGGAGAGAGGATGTACCTGAATTGGAGGAATATTTAAAGAAGCATTTCAGAGAGTTTAGGCATGCCCATTGCTCTCCTTTAAAGCAGGTTATTCATCCCATCCACGACCAGACCTTTTATCTGACTCGAGAGCATAAGAGGAAACTTAAGGAGGAGTATGGAATCGAGCCTTGGACTTTTATTCAGAAGCTAGGAGATGCTGTTTTCATTCCTGCCGGTTGTCCTCACCAAGTCAGAAATCTAAAGTCATGCATCAAGGTTGGACTTGATTTTGTCTCACCTGAAAATGTTGGTGAGTGCTTCCGTTTGACTGAGGAATTCCGTAAGCTTCCGATAAACCACAGGTCCACCAAGGACAGGTTGGAGGTGAAGAAAATGACTATATATGCTATGCTTGATGTGGTTAACAAATTGGAGAAAACAAGGTTACTGATTGCAAACTTCCTCTCCCTTTGGTTATGA
Protein sequence

>MsaT005733.1

MAEEENNHTVTYTVKIKVTLTVPDPDEPIPDHDRRETQPTANVGAETVPLKGDDVAQAIGVRAETALLNNDEGDDVVEAAEESIPDREKQDIDVGAKTVVLLSTDDEGSVEADDMAAKDSDENNAEQECEEKKVIKEKKRAGRKRKKTVYSSEEGEEKNWEGADVEEGGFSSGCVRKRQRPRKIKSLKEDYVSDLEEDMKKKWKRGRKKKVIIEVSDENDEAEQEEEDVKTEQKKPGRKRKVGYGSEENDEDEQEGENGKNFKIVENELISEKNLESDVLSDDNKGYSLRTDRKVKVKSIEQPKQKINKRNPKWIEEESLMCHQCQRNDKGRVVRCTKCKRKRFCIPCLNNWYPHLKEEEVAEACPVCCGNCNCKACLRSCALIKEIKMKTETNNNHEFELSKYMLKELLPYLRRLDEEQMVEKEIEAKRKGLSHSKLKVKAADYPKNKRVYCDNCKTSIFDYHRSCTKCSFNICLLCCCELRCGKLLGGTDPIEFEFIFQGRDYLHGGKEERVKKNKPHSAAQPEICEWSRFGWHADSDGSIPCPKADDDCGHGFLELRSILPPNCISELVYAEETFDSMCSCLKPVRNATDIHNNTRKAASREDSSDNFLYSPRALNLRHEDLRHFQWHWSKGEPVIISNVLECTTGLSWEPLVMWRAFRQIRNTKHKTLLDVEAIDCLDWCEENINVHQFFTGYTNGRPDWLNWPQVLKLKDWPPSNLFRESLPRHCAEFISSLPYKEYTDPFKGILNLAVKLPENVLKPDMGPKTYIAYGFAQELGRGDSVTKLHCNMSDAVNVLTHIAEVKLKSEGIAAIKKLTQKHLEQDKRELHGDNRDGETNVDMFDNSSSSINASDEQNSVRVLENRGDSLDGALWDIFRREDVPELEEYLKKHFREFRHAHCSPLKQVIHPIHDQTFYLTREHKRKLKEEYGIEPWTFIQKLGDAVFIPAGCPHQVRNLKSCIKVGLDFVSPENVGECFRLTEEFRKLPINHRSTKDRLEVKKMTIYAMLDVVNKLEKTRLLIANFLSLWL*