AlfalfaGEDB | MsaG005763

M. sativa ssp. caerulea / MsaG005763

KEGG: K09260
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090406 GO:0120025 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005763	KEH44429.1	92.373	354	20	1	1	354	1	347	0.0	661

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005763	sp\|Q9FIM0\|AGL82_ARATH	30.380	158	105	3	6	160	6	161	4.24e-17	84.0

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005763	tr\|A0A072VR19\|A0A072VR19_MEDTR	92.373	354	20	1	1	354	1	347	0.0	661

TFs/TRs:

Gene ID	Type	Classification
MsaG005763	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG003769	MsaG005763	0.807699	7.562430e-50	1.221840e-47
MsaG004004	MsaG005763	0.806430	1.399605e-49	2.194340e-47
MsaG003938	MsaG005763	0.820980	9.037443e-53	2.039952e-50
MsaG005759	MsaG005763	0.952804	3.626485e-110	1.024099e-104
MsaG005763	MsaG010994	0.804625	3.334050e-49	5.010707e-47
MsaG005763	MsaG011749	0.804195	4.094095e-49	6.092108e-47
MsaG005763	MsaG014232	0.809992	2.458485e-50	4.197578e-48
MsaG005763	MsaG018920	0.804435	3.650227e-49	5.461880e-47
MsaG005763	MsaG020578	0.816542	9.099370e-52	1.829244e-49
MsaG005763	MsaG023817	0.802444	9.395793e-49	1.342777e-46
MsaG005763	MsaG024652	0.806940	1.093712e-49	1.735355e-47
MsaG005763	MsaG029236	0.802902	7.568323e-49	1.092973e-46
MsaG005763	MsaG033702	0.806116	1.628654e-49	2.534418e-47
MsaG005763	MsaG036131	0.803408	5.952533e-49	8.697458e-47
MsaG005763	MsaG041015	0.831215	3.415282e-55	1.023393e-52
MsaG005763	MsaG041017	0.800668	2.164994e-48	2.971758e-46
MsaG005763	MsaG041044	0.821439	7.091311e-53	1.620157e-50
MsaG005763	MsaG041230	0.811902	9.526676e-51	1.704398e-48
MsaG005763	MsaG046526	0.810134	2.291764e-50	3.926453e-48
MsaG005763	MsaG019026	0.835636	2.729011e-56	9.316655e-54
MsaG005763	MsaG032143	0.807757	7.353314e-50	1.189685e-47
MsaG005763	MsaG031245	0.805226	2.499727e-49	3.809666e-47
MsaG005763	MsaG030261	0.828643	1.436582e-54	4.000052e-52
MsaG005763	MsaG037000	0.803681	5.228880e-49	7.688524e-47
MsaG005763	MsaG037532	0.818088	4.099635e-52	8.576328e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005763	MtrunA17_Chr1g0212151	92.373	354	20	1	1	354	1	347	0.0	661
MsaG005763	MtrunA17_Chr6g0457851	73.315	356	83	2	1	354	11	356	0.0	536
MsaG005763	MtrunA17_Chr6g0449861	61.667	360	119	6	1	354	9	355	1.32e-147	421
MsaG005763	MtrunA17_Chr6g0449851	57.307	349	108	4	1	349	1	308	2.62e-135	388
MsaG005763	MtrunA17_Chr4g0059631	41.935	155	87	1	1	155	1	152	5.62e-36	131
MsaG005763	MtrunA17_Chr1g0212471	32.973	185	123	1	1	185	1	184	3.39e-33	122
MsaG005763	MtrunA17_Chr4g0022641	37.126	167	102	1	1	167	1	164	7.64e-31	120
MsaG005763	MtrunA17_Chr2g0303981	38.365	159	93	2	3	160	5	159	2.26e-29	112
MsaG005763	MtrunA17_Chr5g0432691	34.454	119	70	4	1	118	1	112	1.10e-15	73.9
MsaG005763	MtrunA17_Chr3g0134451	33.945	109	64	3	1	109	1	101	2.55e-14	72.8
MsaG005763	MtrunA17_Chr3g0107381	23.478	230	153	8	1	216	1	221	1.19e-13	69.3
MsaG005763	MtrunA17_Chr4g0014321	35.897	117	60	4	1	116	1	103	1.54e-13	69.3
MsaG005763	MtrunA17_Chr8g0379021	30.625	160	84	5	2	144	12	161	3.21e-12	64.3
MsaG005763	MtrunA17_Chr3g0107351	32.759	116	69	4	3	116	2	110	5.58e-12	63.5
MsaG005763	MtrunA17_Chr3g0107341	34.188	117	66	5	3	116	2	110	1.26e-11	62.4
MsaG005763	MtrunA17_Chr2g0284911	28.025	157	98	4	1	154	16	160	1.61e-11	63.9
MsaG005763	MtrunA17_Chr3g0110131	25.100	251	139	8	1	216	1	237	2.05e-11	63.5
MsaG005763	MtrunA17_Chr2g0283941	31.481	108	66	4	2	108	3	103	2.37e-11	63.2
MsaG005763	MtrunA17_Chr1g0197321	29.464	112	66	4	1	105	1	106	3.53e-11	60.8
MsaG005763	MtrunA17_Chr3g0107331	30.909	110	69	3	3	111	2	105	3.67e-11	61.2
MsaG005763	MtrunA17_Chr3g0107361	29.375	160	99	6	3	154	2	155	3.96e-11	60.8
MsaG005763	MtrunA17_Chr8g0376821	35.802	81	40	1	1	69	1	81	5.15e-11	61.2
MsaG005763	MtrunA17_Chr8g0380021	28.289	152	100	3	1	152	17	159	7.56e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005763	AT5G55690.1	39.310	145	80	2	1	144	1	138	2.60e-25	103
MsaG005763	AT5G55690.2	39.310	145	80	2	1	144	1	138	2.60e-25	103
MsaG005763	AT5G58890.1	30.380	158	105	3	6	160	6	161	4.31e-18	84.0
MsaG005763	AT3G18650.1	34.815	135	74	5	16	144	31	157	5.21e-15	75.9
MsaG005763	AT5G23260.3	25.243	206	133	5	1	202	1	189	6.49e-13	68.2
MsaG005763	AT1G65330.1	33.333	111	67	4	3	112	2	106	6.53e-13	68.6
MsaG005763	AT1G65300.1	33.636	110	64	4	5	112	4	106	6.97e-13	68.6
MsaG005763	AT1G22590.2	29.677	155	98	3	1	150	1	149	8.54e-13	66.2
MsaG005763	AT3G05860.3	28.934	197	116	7	1	185	1	185	1.33e-12	67.4
MsaG005763	AT3G05860.2	38.462	78	40	3	1	77	1	71	1.43e-12	66.6
MsaG005763	AT3G05860.1	37.662	77	42	2	1	77	1	71	2.32e-12	67.0
MsaG005763	AT2G28700.1	27.647	170	103	6	1	164	1	156	3.11e-12	67.4
MsaG005763	AT5G23260.1	25.000	216	130	6	1	202	17	214	5.20e-12	65.9
MsaG005763	AT5G26650.1	33.333	111	63	4	5	112	3	105	6.45e-12	66.6
MsaG005763	AT5G23260.2	23.636	220	133	5	1	202	1	203	1.41e-11	64.3
MsaG005763	AT5G23260.4	23.636	220	133	5	1	202	17	219	2.53e-11	63.9
MsaG005763	AT2G40210.1	31.731	104	64	3	1	103	1	98	2.81e-11	64.7
MsaG005763	AT2G03060.3	23.864	176	119	5	1	173	1	164	3.43e-11	64.3
MsaG005763	AT2G03060.7	23.864	176	119	5	1	173	1	164	3.43e-11	64.3
MsaG005763	AT2G03060.1	23.864	176	119	5	1	173	1	164	3.43e-11	64.3
MsaG005763	AT5G27960.1	31.193	109	68	4	5	112	3	105	4.03e-11	63.9
MsaG005763	AT2G03060.2	23.864	176	119	5	1	173	1	164	4.76e-11	63.9
MsaG005763	AT5G13790.2	31.967	122	74	3	1	122	1	113	7.27e-11	62.4
MsaG005763	AT4G09960.5	27.215	158	98	5	1	154	11	155	9.42e-11	62.0

Find 81 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCCTTTCCATATTGATTT+TGG	0.055519	1:+84971798	None:intergenic
CAACATGGATTTCAACTTAA+TGG	0.148999	1:-84971658	MsaT005763.1:CDS
TTGTCCTTAGGCCATGTTTC+AGG	0.193367	1:+84972468	None:intergenic
TTGATGTTAATGGGGCTAAT+TGG	0.199081	1:-84971885	MsaT005763.1:CDS
TACAAGAATATTGATGTTAA+TGG	0.237026	1:-84971895	MsaT005763.1:CDS
AAATCCTCGACCTACAAAAT+TGG	0.237707	1:+84972497	None:intergenic
GCATTTCCATTGTAATAGTA+TGG	0.257144	1:+84971745	None:intergenic
ATGAAAACCCCTTAGGGTTT+TGG	0.259063	1:-84972023	MsaT005763.1:CDS
ACTTCCGTGATTTCTCCTTT+TGG	0.266267	1:+84972613	None:intergenic
ACAAGAATATTGATGTTAAT+GGG	0.303821	1:-84971894	MsaT005763.1:CDS
GGTTATCAGGACCGATGCTT+CGG	0.326389	1:-84971826	MsaT005763.1:CDS
GATATTATTTGCTAGCAAAC+TGG	0.357133	1:-84972247	MsaT005763.1:CDS
AATGAATCTTTCAATTCTCT+TGG	0.359377	1:-84972282	MsaT005763.1:CDS
GATTTGAGAGATTCTGAAAA+GGG	0.362382	1:+84971683	None:intergenic
GGGTCGTGGAAGAATATCTA+TGG	0.367126	1:-84972643	MsaT005763.1:CDS
TGATGTTAATGGGGCTAATT+GGG	0.367522	1:-84971884	MsaT005763.1:CDS
ATTAACATCAATATTCTTGT+AGG	0.368101	1:+84971897	None:intergenic
TGGGGCTAATTGGGCCAATC+AGG	0.372470	1:-84971875	MsaT005763.1:CDS
TCTTGAACATCATAGATCTT+AGG	0.373714	1:+84972390	None:intergenic
ATGGTGCGAGCTTTGTATAT+TGG	0.377737	1:+84971957	None:intergenic
CGGGCTTCCCAAAATCAATA+TGG	0.380849	1:-84971806	MsaT005763.1:CDS
ATCATTAGCATTAACCTGAT+TGG	0.383785	1:+84971861	None:intergenic
GTTATCAGGACCGATGCTTC+GGG	0.385864	1:-84971825	MsaT005763.1:CDS
TATGCTAAAAGAAGATCATA+AGG	0.417664	1:-84972190	MsaT005763.1:CDS
ATTAGTGATGAAAACCCCTT+AGG	0.421326	1:-84972030	MsaT005763.1:CDS
TCATCACTAATATTCATCAT+TGG	0.425152	1:+84972042	None:intergenic
AAGGAAGGATGGAGCTGAAA+AGG	0.426747	1:-84971782	MsaT005763.1:CDS
AACATCAACATCACAAAGAA+TGG	0.436469	1:+84972539	None:intergenic
ACTATAACACAACAAATGAT+AGG	0.448494	1:-84972416	MsaT005763.1:CDS
AGATTTGAGAGATTCTGAAA+AGG	0.451030	1:+84971682	None:intergenic
AATCCTCGACCTACAAAATT+GGG	0.455489	1:+84972498	None:intergenic
TTAACATCAATATTCTTGTA+GGG	0.460944	1:+84971898	None:intergenic
GGGAGAGTGTGCAAGGCAAT+AGG	0.469982	1:+84971703	None:intergenic
ATGCTAATGATACCTACAAT+GGG	0.473473	1:-84971849	MsaT005763.1:CDS
TAGTTTCTCTTTACGCACTT+TGG	0.478267	1:+84972326	None:intergenic
CAAGCTCATATGTTCATGAA+TGG	0.478657	1:-84971613	MsaT005763.1:CDS
ATCTATGGAACTCATCCAAA+AGG	0.478947	1:-84972628	MsaT005763.1:CDS
GAAGGTTGAGAGCTTTGTCC+CGG	0.483719	1:+84971991	None:intergenic
TTGAAAGATTCATTCCAAGT+TGG	0.490806	1:+84972291	None:intergenic
ATCAATATGGAAAGGAAGGA+TGG	0.505258	1:-84971793	MsaT005763.1:CDS
CAAAATCAATATGGAAAGGA+AGG	0.508161	1:-84971797	MsaT005763.1:CDS
TTAGTGATGAAAACCCCTTA+GGG	0.508185	1:-84972029	MsaT005763.1:CDS
GTCAACATTTCAAAAGAGAA+AGG	0.514992	1:-84972592	MsaT005763.1:CDS
TTGAAGTGGCCAAAACCCTA+AGG	0.517435	1:+84972014	None:intergenic
TCACTAATATTCATCATTGG+AGG	0.523765	1:+84972045	None:intergenic
CTTGGATTTGCGGTCAAGGA+AGG	0.534446	1:+84972117	None:intergenic
CTCATATGTTCATGAATGGA+AGG	0.537554	1:-84971609	MsaT005763.1:CDS
TCGTCACCATACTATTACAA+TGG	0.539170	1:-84971751	MsaT005763.1:CDS
TAAAGATGATGTATCCAACT+TGG	0.548629	1:-84972305	MsaT005763.1:CDS
GTAACTTGGATTTGCGGTCA+AGG	0.551618	1:+84972113	None:intergenic
ATGAGATTGAAGTAGTAACT+TGG	0.551706	1:+84972099	None:intergenic
ATCTTTCAATTCTCTTGGAG+CGG	0.552140	1:-84972277	MsaT005763.1:CDS
ACTTAATGGATTCAATGCTA+AGG	0.552446	1:-84971644	MsaT005763.1:CDS
GAAGTAGTAACTTGGATTTG+CGG	0.553040	1:+84972107	None:intergenic
ATATTGGAAATCAAAGAAGA+AGG	0.553651	1:+84971973	None:intergenic
ATGCTAAAAGAAGATCATAA+GGG	0.556585	1:-84972189	MsaT005763.1:CDS
ACATTTCAAAAGAGAAAGGA+AGG	0.560555	1:-84972588	MsaT005763.1:CDS
GAGCTTTGTCCCGGTTGAAG+TGG	0.560628	1:+84972000	None:intergenic
AATGCTAATGATACCTACAA+TGG	0.561788	1:-84971850	MsaT005763.1:CDS
TGAAGTGGCCAAAACCCTAA+GGG	0.571128	1:+84972015	None:intergenic
TATTCGTAGAAAATGGGTCG+TGG	0.573273	1:-84972657	None:intergenic
TTCCCAAAATCAATATGGAA+AGG	0.576621	1:-84971801	MsaT005763.1:CDS
CTCGCACCATGTGGAGTCTG+AGG	0.578924	1:-84971944	MsaT005763.1:CDS
CTGAAAAGGGAGAGTGTGCA+AGG	0.587535	1:+84971696	None:intergenic
CACCATGTGGAGTCTGAGGA+GGG	0.590680	1:-84971940	MsaT005763.1:CDS
TAACATCAATATTCTTGTAG+GGG	0.591713	1:+84971899	None:intergenic
TCATCCAAAAGGAGAAATCA+CGG	0.593512	1:-84972617	MsaT005763.1:CDS
ATATGTTTGTGCTTGAGACA+TGG	0.597890	1:+84972068	None:intergenic
CAAGAATATTGATGTTAATG+GGG	0.607609	1:-84971893	MsaT005763.1:CDS
TGAACCTGAAACATGGCCTA+AGG	0.608253	1:-84972472	MsaT005763.1:CDS
CTATTACAATGGAAATGCAA+TGG	0.613734	1:-84971740	MsaT005763.1:CDS
GCACCATGTGGAGTCTGAGG+AGG	0.614956	1:-84971941	MsaT005763.1:CDS
GATTTGCTGAACCTGAAACA+TGG	0.621087	1:-84972479	MsaT005763.1:CDS
TACCCTCCTCAGACTCCACA+TGG	0.630866	1:+84971938	None:intergenic
GAAGTGGCCAAAACCCTAAG+GGG	0.646061	1:+84972016	None:intergenic
AATCTCTCAAATCTACAACA+TGG	0.661149	1:-84971673	MsaT005763.1:CDS
TATACAAAGCTCGCACCATG+TGG	0.663687	1:-84971953	MsaT005763.1:CDS
GCTAATGATACCTACAATGG+GGG	0.667333	1:-84971847	MsaT005763.1:CDS
TGCTAATGATACCTACAATG+GGG	0.672222	1:-84971848	MsaT005763.1:CDS
ATGGCCTAAGGACAAAAGAG+AGG	0.693604	1:-84972460	MsaT005763.1:CDS
AATAGCTCATGAATCACACA+AGG	0.705760	1:-84972160	MsaT005763.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	84971603	84972667	84971603	ID=MsaG005763
Chr1	mRNA	84971603	84972667	84971603	ID=MsaT005763.1;Parent=MsaG005763
Chr1	exon	84971603	84972667	84971603	ID=MsaT005763.1.exon1;Parent=MsaT005763.1
Chr1	CDS	84971603	84972667	84971603	ID=cds.MsaT005763.1;Parent=MsaT005763.1

Gene Sequence

>MsaT005763.1

ATGGGTCGTGGAAGAATATCTATGGAACTCATCCAAAAGGAGAAATCACGGAAGTCAACATTTCAAAAGAGAAAGGAAGGATTAATGAAAAAAGTGAATGAATTTTCCATTCTTTGTGATGTTGATGTTTGTGTAGTTCTCTATGCTCCCAATTTTGTAGGTCGAGGATTTGCTGAACCTGAAACATGGCCTAAGGACAAAAGAGAGGTGCATAGAATCCTTCAAAAGTACTATAACACAACAAATGATAGGCGTCCTAAGATCTATGATGTTCAAGAGTATTTTAAGGAAAGGATGAGAAAACTTGAATTTGAGATTTCCAAAGTGCGTAAAGAGAAACTAAAGATGATGTATCCAACTTGGAATGAATCTTTCAATTCTCTTGGAGCGGAACAACTGATATTATTTGCTAGCAAACTGGAAGCTAAGCTTGATGCTTGTAATCAAAAGAAGCATATGCTAAAAGAAGATCATAAGGGAAAAACAATAGCTCATGAATCACACAAGGTAGATAAACTTATCAGTACTCCTTCCTTGACCGCAAATCCAAGTTACTACTTCAATCTCATGCAAAATTCCATGTCTCAAGCACAAACATATCCTCCAATGATGAATATTAGTGATGAAAACCCCTTAGGGTTTTGGCCACTTCAACCGGGACAAAGCTCTCAACCTTCTTCTTTGATTTCCAATATACAAAGCTCGCACCATGTGGAGTCTGAGGAGGGTAGATTTGTGCAGTCTTACCCCTACAAGAATATTGATGTTAATGGGGCTAATTGGGCCAATCAGGTTAATGCTAATGATACCTACAATGGGGGTTATCAGGACCGATGCTTCGGGCTTCCCAAAATCAATATGGAAAGGAAGGATGGAGCTGAAAAGGATGAAAACTCGTCACCATACTATTACAATGGAAATGCAATGGCAATGCAATCAAATCCTATTGCCTTGCACACTCTCCCTTTTCAGAATCTCTCAAATCTACAACATGGATTTCAACTTAATGGATTCAATGCTAAGGATATTCTGCAAGCTCATATGTTCATGAATGGAAGGAAGTAG

Protein sequence

>MsaT005763.1

MGRGRISMELIQKEKSRKSTFQKRKEGLMKKVNEFSILCDVDVCVVLYAPNFVGRGFAEPETWPKDKREVHRILQKYYNTTNDRRPKIYDVQEYFKERMRKLEFEISKVRKEKLKMMYPTWNESFNSLGAEQLILFASKLEAKLDACNQKKHMLKEDHKGKTIAHESHKVDKLISTPSLTANPSYYFNLMQNSMSQAQTYPPMMNISDENPLGFWPLQPGQSSQPSSLISNIQSSHHVESEEGRFVQSYPYKNIDVNGANWANQVNANDTYNGGYQDRCFGLPKINMERKDGAEKDENSSPYYYNGNAMAMQSNPIALHTLPFQNLSNLQHGFQLNGFNAKDILQAHMFMNGRK*