AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005779

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005779 MtrunA17_Chr1g0211661 93.179 865 58 1 1 864 1 865 0.0 1594
MsaG005779 MtrunA17_Chr1g0211711 68.961 799 234 5 143 930 262 1057 0.0 1123
MsaG005779 MtrunA17_Chr1g0211711 49.693 163 60 5 42 195 7 156 2.68e-25 113
MsaG005779 MtrunA17_Chr1g0183311 60.506 909 287 10 69 930 5 888 0.0 1053
MsaG005779 MtrunA17_Chr5g0426961 69.510 715 209 3 213 925 129 836 0.0 1009
MsaG005779 MtrunA17_Chr1g0211691 62.640 712 229 10 135 812 237 945 0.0 865
MsaG005779 MtrunA17_Chr1g0211691 63.131 198 65 2 1 194 1 194 1.56e-58 217
MsaG005779 MtrunA17_Chr5g0420361 62.500 648 206 8 288 905 139 779 0.0 806
MsaG005779 MtrunA17_Chr5g0448301 72.401 558 131 3 278 834 96 631 0.0 798
MsaG005779 MtrunA17_Chr5g0448301 46.635 208 84 7 40 241 2 188 6.60e-31 129
MsaG005779 MtrunA17_Chr6g0472441 58.974 663 206 12 288 917 81 710 0.0 759
MsaG005779 MtrunA17_Chr2g0289121 46.591 704 330 11 224 921 156 819 0.0 592
MsaG005779 MtrunA17_Chr4g0075261 43.514 740 333 15 224 921 168 864 0.0 585
MsaG005779 MtrunA17_Chr7g0275771 43.214 641 330 12 139 752 43 676 1.29e-157 487
MsaG005779 MtrunA17_Chr7g0275771 59.627 161 65 0 770 930 773 933 1.26e-55 208
MsaG005779 MtrunA17_Chr1g0192011 35.135 740 394 11 224 922 267 961 5.25e-149 466
MsaG005779 MtrunA17_Chr1g0147021 45.386 531 250 10 224 748 870 1366 2.90e-132 436
MsaG005779 MtrunA17_Chr1g0147021 68.382 136 43 0 777 912 1551 1686 2.35e-54 206
MsaG005779 MtrunA17_Chr2g0289031 53.299 394 174 3 530 921 8 393 5.23e-132 402
MsaG005779 MtrunA17_Chr2g0281051 34.115 768 426 16 224 926 207 959 3.35e-127 408
MsaG005779 MtrunA17_Chr4g0048531 36.250 560 265 13 446 922 727 1277 6.54e-100 340
MsaG005779 MtrunA17_Chr4g0048531 37.143 175 100 4 224 389 260 433 3.88e-28 122
MsaG005779 MtrunA17_Chr8g0364421 51.550 258 94 3 334 591 5 231 2.20e-75 246
MsaG005779 MtrunA17_Chr7g0231061 57.923 183 72 2 590 769 11 191 4.08e-61 206
MsaG005779 MtrunA17_Chr1g0211701 76.033 121 29 0 810 930 4 124 9.22e-58 195
MsaG005779 MtrunA17_Chr8g0361381 52.973 185 83 2 528 712 28 208 1.18e-55 191
MsaG005779 MtrunA17_Chr2g0289091 64.167 120 41 1 769 886 301 420 1.64e-43 164
MsaG005779 MtrunA17_Chr2g0289091 55.634 142 62 1 604 745 23 163 3.04e-43 163
MsaG005779 MtrunA17_Chr2g0289041 37.915 211 122 5 246 455 2 204 4.94e-40 147
MsaG005779 MtrunA17_Chr6g0469821 58.219 146 47 3 1 138 1 140 2.75e-39 142
MsaG005779 MtrunA17_Chr8g0364411 56.250 112 49 0 224 335 33 144 3.57e-35 130
MsaG005779 MtrunA17_Chr8g0361391 67.416 89 28 1 776 864 50 137 3.99e-35 131
MsaG005779 MtrunA17_Chr1g0211671 96.774 62 2 0 863 924 15 76 3.59e-33 124
MsaG005779 MtrunA17_Chr8g0340381 54.930 142 41 4 625 762 36 158 9.17e-33 125
MsaG005779 MtrunA17_Chr1g0211681 53.261 92 35 2 55 138 1 92 1.02e-17 79.3
MsaG005779 MtrunA17_Chr2g0289101 36.923 130 76 3 353 481 5 129 4.51e-17 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005779 AT1G11950.1 48.442 706 336 11 219 921 188 868 0.0 645
MsaG005779 AT1G11950.3 48.442 706 336 11 219 921 188 868 0.0 645
MsaG005779 AT1G62310.1 49.062 693 331 10 223 910 208 883 0.0 640
MsaG005779 AT1G11950.2 47.261 639 309 11 219 854 188 801 0.0 558
MsaG005779 AT3G07610.1 56.008 491 205 4 436 925 415 895 4.34e-177 541
MsaG005779 AT3G07610.1 35.540 287 175 3 133 410 55 340 1.64e-47 184
MsaG005779 AT3G07610.3 53.725 510 209 5 436 925 415 917 1.00e-168 520
MsaG005779 AT3G07610.3 35.540 287 175 3 133 410 55 340 1.53e-47 184
MsaG005779 AT4G00990.3 40.992 605 313 14 155 740 2 581 5.40e-135 423
MsaG005779 AT4G00990.3 61.053 95 37 0 776 870 684 778 1.34e-30 130
MsaG005779 AT4G00990.2 40.992 605 313 14 155 740 2 581 6.76e-135 423
MsaG005779 AT4G00990.2 65.517 87 30 0 776 862 684 770 3.06e-30 129
MsaG005779 AT4G00990.1 40.992 605 313 14 155 740 2 581 1.45e-134 424
MsaG005779 AT4G00990.1 61.379 145 56 0 776 920 684 828 6.61e-52 196
MsaG005779 AT1G09060.2 30.641 842 502 15 115 922 98 891 2.54e-120 389
MsaG005779 AT1G09060.1 30.641 842 502 15 115 922 98 891 2.54e-120 389
MsaG005779 AT1G09060.4 30.641 842 502 15 115 922 112 905 2.54e-120 389
MsaG005779 AT1G09060.3 30.641 842 502 15 115 922 112 905 2.54e-120 389
MsaG005779 AT3G07610.2 56.890 283 115 2 436 718 415 690 5.58e-94 314
MsaG005779 AT3G07610.2 35.540 287 175 3 133 410 55 340 1.35e-47 182
MsaG005779 AT4G21430.1 34.475 438 248 5 521 925 485 916 1.20e-79 278
MsaG005779 AT4G21430.1 29.299 157 108 3 224 378 227 382 1.24e-11 69.3

Find 217 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAGATATTCTTTCAATT+TGG 0.093161 1:-85151573 None:intergenic
GCCGATTCTACCTGCTTTAA+AGG 0.132025 1:-85151632 None:intergenic
ATATCACTCTCTTTCAATTT+AGG 0.147719 1:-85147841 None:intergenic
GCCTCCTTCTGATTTATTTC+AGG 0.172247 1:+85150622 MsaT005779.1:CDS
AACCAAAACTACATCGTATT+TGG 0.218834 1:-85149072 None:intergenic
AAATCATACTCAAGCTTAAT+TGG 0.220679 1:-85148507 None:intergenic
TCACATTCATTATCAGCTTT+TGG 0.248622 1:-85148669 None:intergenic
GGATTTGTGTATTCTTTATA+AGG 0.268369 1:-85150686 None:intergenic
GTCGCAGCGATTGGCTTAAA+TGG 0.273010 1:+85150573 MsaT005779.1:CDS
AGTACATTGCTGACAATTAC+AGG 0.278275 1:-85148981 None:intergenic
CCAAGAACATATATTGCTTA+TGG 0.280860 1:+85150773 MsaT005779.1:CDS
ATCCCAGAGAGCACCTTCTA+AGG 0.286251 1:-85151528 None:intergenic
TGTCATTTCTTTGACATTGA+TGG 0.286327 1:-85147552 None:intergenic
CTCCAAATACGATGTAGTTT+TGG 0.286573 1:+85149070 MsaT005779.1:CDS
CTCCGTGGTGGGAAGCTTCT+AGG 0.287089 1:+85148474 MsaT005779.1:CDS
TCTAGATATTCTTTCAATTT+GGG 0.290597 1:-85151572 None:intergenic
ACTAACTCAGAGATGCAATT+TGG 0.317312 1:-85148726 None:intergenic
CGACAGCGACACCACCGTTT+TGG 0.322177 1:-85147070 None:intergenic
TCAGACCATTTATCTGAATA+TGG 0.327236 1:+85152098 MsaT005779.1:CDS
CCATAAGCAATATATGTTCT+TGG 0.328103 1:-85150773 None:intergenic
CAACCTTGCTGTGAAGTTGC+CGG 0.339593 1:+85150724 MsaT005779.1:CDS
ATGCTCCATATTCAGATAAA+TGG 0.340188 1:-85152103 None:intergenic
AGGGATGCAGAAGCGTTGTT+AGG 0.362991 1:+85147237 MsaT005779.1:CDS
ATGAAACTTAAGAAAGAGTA+TGG 0.363484 1:+85152129 MsaT005779.1:CDS
ATTCGTGAGTGGTCAAGATC+TGG 0.381498 1:+85148612 MsaT005779.1:CDS
ATCCCTTAGAAGGTGCTCTC+TGG 0.383194 1:+85151525 MsaT005779.1:CDS
CTAAAGATGAGCGTGTGTTC+TGG 0.394804 1:+85148284 MsaT005779.1:CDS
CTCAAAGCTAAATATAAAAC+CGG 0.396606 1:+85148252 MsaT005779.1:CDS
CTCAACCTCCTCCTCGTTGT+TGG 0.396999 1:-85148045 None:intergenic
GTTCTTGCTCGAAACCTGTT+AGG 0.397008 1:+85148817 MsaT005779.1:CDS
TAGAGGAGACTCGTGATAAT+AGG 0.397753 1:+85148793 MsaT005779.1:CDS
TGATCAGGAATGGGTTCATC+TGG 0.401180 1:-85146856 None:intergenic
ATCCTCCAACGGTACCGTTT+CGG 0.404627 1:-85146917 None:intergenic
ATATCATCGGTGTACCTAAC+AGG 0.407455 1:-85148831 None:intergenic
AGGCAGTTAATTGTTTAGAT+TGG 0.408112 1:+85149099 MsaT005779.1:CDS
TTCAATGGCACTGGAGCAAA+GGG 0.413215 1:+85148955 MsaT005779.1:CDS
TATGTTCTTGGCCCCATGTC+AGG 0.417723 1:-85150761 None:intergenic
AATATCCACCAATTCTTCAC+TGG 0.420686 1:+85150539 MsaT005779.1:CDS
GACGATGAAGGTTCGGTTGA+AGG 0.422097 1:+85147096 MsaT005779.1:CDS
AGGTTGAGAGAACCACTAAA+AGG 0.429360 1:-85150707 None:intergenic
CAGAAATTGAAAAGATGAAG+AGG 0.429960 1:+85147217 MsaT005779.1:CDS
CGAGGTCGCAATTATCTACA+TGG 0.434006 1:+85148531 MsaT005779.1:CDS
AATGTACTTGAATGCACATC+TGG 0.441844 1:+85148996 MsaT005779.1:CDS
TTTGTCTCTCCCGAACATAT+TGG 0.442105 1:+85153688 MsaT005779.1:CDS
AGGCTCTTCATCATTTCAAC+TGG 0.442693 1:+85147302 MsaT005779.1:CDS
GGAATGGGTTCATCTGGATC+AGG 0.444988 1:-85146850 None:intergenic
GCAGCGGATCTGTGATTTAT+CGG 0.450090 1:-85153748 None:intergenic
AACAGAATAAAGGAAGAAAC+TGG 0.451192 1:+85147261 MsaT005779.1:CDS
GTTGGAGAGGATCCCTTAGA+AGG 0.451802 1:+85151515 MsaT005779.1:CDS
GAAGCACTCACCAATATGTT+CGG 0.452411 1:-85153698 None:intergenic
TACCGTTTCGGCGCCAACAT+TGG 0.457827 1:-85146905 None:intergenic
TTCTTCACTGGGTACACAAA+TGG 0.460684 1:+85150551 MsaT005779.1:CDS
CGCCGAAACGGTACCGTTGG+AGG 0.461207 1:+85146915 MsaT005779.1:CDS
TCTTCCACATTTAAGACGGT+TGG 0.461419 1:+85148094 MsaT005779.1:CDS
GAAGTATCCTTCTCAGTTCA+AGG 0.464781 1:-85148701 None:intergenic
AGCACCTTGAGCAAGATAAG+AGG 0.465265 1:+85151420 MsaT005779.1:CDS
TTAATGACACTAATTTCCTC+AGG 0.465562 1:-85151383 None:intergenic
GGCCAATGTTGGCGCCGAAA+CGG 0.467500 1:+85146903 MsaT005779.1:CDS
TTACCTTCAGATTTCTGACT+TGG 0.467821 1:-85152317 None:intergenic
AATGCACATCTGGTTTAAGC+TGG 0.467845 1:+85149006 MsaT005779.1:CDS
TCTTTCAATTTCAACACCTC+AGG 0.472947 1:-85150596 None:intergenic
GTTTCTCGATGATCATGATC+AGG 0.474522 1:-85146871 None:intergenic
ATTTGGAGTTGGTTATTTGA+CGG 0.476343 1:-85149055 None:intergenic
TTGTGTACCCAGTGAAGAAT+TGG 0.478196 1:-85150547 None:intergenic
TTAAGTTTCTTGTGGACTTG+TGG 0.479397 1:-85147384 None:intergenic
GACTTGTAGGAATTGAGCCT+TGG 0.481982 1:+85152241 MsaT005779.1:intron
ATCCGCTGCGGACAAGTTTG+AGG 0.482235 1:+85153762 MsaT005779.1:CDS
ACACAAATGGTCGCAGCGAT+TGG 0.484713 1:+85150564 MsaT005779.1:CDS
AGCTGAAACGTCGTCGTTGA+AGG 0.484777 1:+85146969 MsaT005779.1:CDS
CGATGATCATGATCAGGAAT+GGG 0.491693 1:-85146865 None:intergenic
AGGTGATAACATTGCAGCTA+GGG 0.495234 1:+85147116 MsaT005779.1:CDS
TCGATGATCATGATCAGGAA+TGG 0.497827 1:-85146866 None:intergenic
TCCCTTAGAAGGTGCTCTCT+GGG 0.498565 1:+85151526 MsaT005779.1:CDS
ATCAAGTGCAACCTTGATGC+AGG 0.498619 1:-85153665 None:intergenic
GTTGTTAGGGAACAGAATAA+AGG 0.500566 1:+85147251 MsaT005779.1:CDS
CACATCAAGCATAGCATGTA+CGG 0.501504 1:-85154393 None:intergenic
ATCAATGTCAAAGAAATGAC+AGG 0.501804 1:+85147554 MsaT005779.1:CDS
GAGACGCGGCAGAAAGAGAA+AGG 0.502047 1:+85147332 MsaT005779.1:CDS
TGCGGAATTCCTCTGTCAAA+CGG 0.503979 1:-85153720 None:intergenic
GCACATCATGTATACAAAAG+TGG 0.504161 1:+85147620 MsaT005779.1:CDS
GTCGAAGGACGATGAAGGTT+CGG 0.505191 1:+85147089 MsaT005779.1:CDS
AGGTGTTGAAATTGAAAGAT+TGG 0.508050 1:+85150600 MsaT005779.1:CDS
AGGGATCCTCTCCAACCATC+AGG 0.508501 1:-85151508 None:intergenic
GTATATTTACGCACAAACCT+TGG 0.508550 1:-85154665 None:intergenic
AACTACATCGTATTTGGAGT+TGG 0.509194 1:-85149066 None:intergenic
GGGATGCAGAAGCGTTGTTA+GGG 0.509891 1:+85147238 MsaT005779.1:CDS
TTTCAATGGCACTGGAGCAA+AGG 0.515097 1:+85148954 MsaT005779.1:CDS
GTTGACTCATATCGCTAAAG+TGG 0.516636 1:+85151352 MsaT005779.1:CDS
ACTGGTAAAGATGTGGTAGC+TGG 0.518953 1:+85147279 MsaT005779.1:CDS
TCAGGCTTTACACAATAATC+CGG 0.522487 1:-85150743 None:intergenic
CGCAGCAACAGAGGAGACAA+AGG 0.527535 1:-85148449 None:intergenic
AAGCACTCACCAATATGTTC+GGG 0.527844 1:-85153697 None:intergenic
GCTGATAATGAATGTGATCA+TGG 0.528933 1:+85148675 MsaT005779.1:CDS
TCTGTTGCAGTCCTGCATCA+AGG 0.530126 1:+85153654 MsaT005779.1:intron
TGAAGAACAAATGATTGAGA+AGG 0.530667 1:+85148118 MsaT005779.1:CDS
AAGAGGGAGCTACATGGTGA+TGG 0.531116 1:+85151437 MsaT005779.1:CDS
GTTGCTGCGAGCTCCGTGGT+GGG 0.531732 1:+85148463 MsaT005779.1:CDS
GATAAATGGTCTGATCATGA+AGG 0.531871 1:-85152089 None:intergenic
AGAGGGAGCTACATGGTGAT+GGG 0.532113 1:+85151438 MsaT005779.1:CDS
ATGGTTTCCTTGAACTGAGA+AGG 0.533086 1:+85148694 MsaT005779.1:CDS
CAAACCAGCAGGAATGAAAA+CGG 0.534926 1:-85152289 None:intergenic
GCTACATGGTGATGGGGAGG+CGG 0.535164 1:+85151445 MsaT005779.1:CDS
TGTGTATTCTTTATAAGGCA+AGG 0.535177 1:-85150681 None:intergenic
TATGGATTTACTCAAAATCT+CGG 0.535793 1:+85150791 MsaT005779.1:CDS
TATAGGAATACCTCGTCTCC+TGG 0.539286 1:-85148150 None:intergenic
TATGCATGTTGGCGCCAAAA+CGG 0.540397 1:+85147056 MsaT005779.1:CDS
AACGGTGGTGTCGCTGTCGA+AGG 0.540573 1:+85147074 MsaT005779.1:CDS
TGTGTTATTGTGTATATCAT+CGG 0.540704 1:-85148844 None:intergenic
TTCGTGAGTGGTCAAGATCT+GGG 0.542666 1:+85148613 MsaT005779.1:CDS
CAATTGCATTCCTTTAAAGC+AGG 0.542700 1:+85151622 MsaT005779.1:CDS
TCGGCGCCAACATTGGCCGT+AGG 0.543112 1:-85146898 None:intergenic
GCTCCCTCTTATCTTGCTCA+AGG 0.544578 1:-85151424 None:intergenic
ACTTGGTGAGGCAAACCAGC+AGG 0.547834 1:-85152300 None:intergenic
GCACCTTGAGCAAGATAAGA+GGG 0.549030 1:+85151421 MsaT005779.1:CDS
TTAGATGCACAAAATGCAAA+CGG 0.550175 1:+85147587 MsaT005779.1:CDS
TCCCAGAGAGCACCTTCTAA+GGG 0.550365 1:-85151527 None:intergenic
TCATCCAACCGTCTTAAATG+TGG 0.550657 1:-85148098 None:intergenic
AACTCAGAGATGCAATTTGG+AGG 0.551495 1:-85148723 None:intergenic
TGGTGTAGATCTACAGCCCT+TGG 0.555022 1:-85148915 None:intergenic
TTGACATTGATGGCACATTG+AGG 0.559359 1:-85147542 None:intergenic
GTGGCACAAGCTATCGGTGT+TGG 0.561909 1:+85146946 MsaT005779.1:CDS
TGTTGCTGCGAGCTCCGTGG+TGG 0.562600 1:+85148462 MsaT005779.1:CDS
ATATCCACCAATTCTTCACT+GGG 0.564592 1:+85150540 MsaT005779.1:CDS
TTCTCTAAGTATCTGCTGAA+AGG 0.564863 1:+85148068 MsaT005779.1:CDS
TTCAGATTTCTGACTTGGTG+AGG 0.565040 1:-85152312 None:intergenic
GACTTCTTCCACATTTAAGA+CGG 0.566290 1:+85148090 MsaT005779.1:CDS
AGAAATTGAAAAGATGAAGA+GGG 0.567266 1:+85147218 MsaT005779.1:CDS
TGTACCCAGTGAAGAATTGG+TGG 0.567688 1:-85150544 None:intergenic
ATTTGAAATTTCAATGGCGG+AGG 0.570807 1:+85146771 None:intergenic
AAGGTGATAACATTGCAGCT+AGG 0.571180 1:+85147115 MsaT005779.1:CDS
ACACAACCTACGGCCAATGT+TGG 0.573109 1:+85146892 MsaT005779.1:CDS
ACAGCACACAGGGCATGCCT+TGG 0.575809 1:-85147866 None:intergenic
CAAGATAAGAGGGAGCTACA+TGG 0.577423 1:+85151431 MsaT005779.1:CDS
ATGCACATCTGGTTTAAGCT+GGG 0.577804 1:+85149007 MsaT005779.1:CDS
TGTCAAAGAAATGACAGGGC+CGG 0.578080 1:+85147559 MsaT005779.1:CDS
CAACTCTCTGACACTAATGA+TGG 0.579184 1:+85151482 MsaT005779.1:CDS
CGCCGCCGCAGCTTTAGCAG+AGG 0.580166 1:-85147145 None:intergenic
CACGGAGCTCGCAGCAACAG+AGG 0.580415 1:-85148458 None:intergenic
GGAGCTACATGGTGATGGGG+AGG 0.581361 1:+85151442 MsaT005779.1:CDS
TGCAATTATCACAGCACACA+GGG 0.584315 1:-85147876 None:intergenic
ACTATAGGAAATGAAGCGTA+AGG 0.584966 1:+85148016 MsaT005779.1:intron
GGTGACGTGGCACAAGCTAT+CGG 0.585670 1:+85146940 MsaT005779.1:CDS
ATAAATGGTCTGATCATGAA+GGG 0.587122 1:-85152088 None:intergenic
CATCATTAGTGTCAGAGAGT+TGG 0.587536 1:-85151481 None:intergenic
AATCAAGCTTCAAGATGTAG+AGG 0.589159 1:+85148776 MsaT005779.1:CDS
TATTGTGTAAAGCCTGACAT+GGG 0.589216 1:+85150749 MsaT005779.1:CDS
TGAAACCAACAACGAGGAGG+AGG 0.589775 1:+85148040 MsaT005779.1:CDS
AGTTCCTGAAATAAATCAGA+AGG 0.590173 1:-85150626 None:intergenic
GAATGGGTTCATCTGGATCA+GGG 0.590909 1:-85146849 None:intergenic
CACTAATGATGGTGACCTGA+TGG 0.592137 1:+85151493 MsaT005779.1:CDS
TGGACAAGGAATGCTACCAT+CGG 0.593451 1:-85148649 None:intergenic
GCAATTGCAAAGCATGCTTG+CGG 0.593478 1:+85147896 MsaT005779.1:CDS
TGGCGCCGAAACGGTACCGT+TGG 0.594492 1:+85146912 MsaT005779.1:CDS
AATCAGCGAGAGTAATGCTA+AGG 0.594627 1:+85147407 MsaT005779.1:CDS
TTACAGGTCTGGGAAAGCCA+AGG 0.595635 1:+85154648 MsaT005779.1:intron
TTGAGAAGGAAAGAGAAGCC+AGG 0.596035 1:+85148132 MsaT005779.1:CDS
GAAAGAGAAGCCAGGAGACG+AGG 0.596795 1:+85148140 MsaT005779.1:CDS
AATGATGGTGACCTGATGGT+TGG 0.598278 1:+85151497 MsaT005779.1:CDS
AACTTCTGAATAAAAGTCCA+AGG 0.598579 1:-85152258 None:intergenic
AGAAAGTGTATCACATGCAG+AGG 0.606509 1:+85148578 MsaT005779.1:CDS
CACCTAGAAGCTTCCCACCA+CGG 0.608942 1:-85148476 None:intergenic
TCATCGAGAAACACAACCTA+CGG 0.610549 1:+85146882 MsaT005779.1:CDS
GGGTTCATCTGGATCAGGGA+GGG 0.610921 1:-85146845 None:intergenic
TGTGATTTATCGGAAGCTTG+CGG 0.611620 1:-85153738 None:intergenic
GGAAGAAACTGGTAAAGATG+TGG 0.613492 1:+85147272 MsaT005779.1:CDS
GAAACGGTACCGTTGGAGGA+TGG 0.613747 1:+85146919 MsaT005779.1:CDS
TTATTGTGTAAAGCCTGACA+TGG 0.616770 1:+85150748 MsaT005779.1:CDS
CTCTGTTGCTGCGAGCTCCG+TGG 0.620459 1:+85148459 MsaT005779.1:CDS
TTGCAATTATCACAGCACAC+AGG 0.625183 1:-85147877 None:intergenic
GAAAGAGAGTGATATTGCCA+AGG 0.625820 1:+85147849 MsaT005779.1:CDS
TCGCTGTCGAAGGACGATGA+AGG 0.626327 1:+85147084 MsaT005779.1:CDS
TCCTGAAATAAATCAGAAGG+AGG 0.633909 1:-85150623 None:intergenic
TATACACAATAACACAAGGA+AGG 0.636388 1:+85148851 MsaT005779.1:CDS
ATCCTCTGCTAAAGCTGCGG+CGG 0.638471 1:+85147143 MsaT005779.1:CDS
TCACCAAGTCAGAAATCTGA+AGG 0.640211 1:+85152314 MsaT005779.1:CDS
CGTACATGCTATGCTTGATG+TGG 0.642539 1:+85154394 MsaT005779.1:CDS
AACCTCAAACTTGTCCGCAG+CGG 0.644100 1:-85153764 None:intergenic
AATGCACGCCACATGACTAG+TGG 0.646851 1:-85149032 None:intergenic
ACCGTTGGAGGATGGTGACG+TGG 0.648574 1:+85146927 MsaT005779.1:CDS
GGTAAAGATGTGGTAGCTGG+AGG 0.648889 1:+85147282 MsaT005779.1:CDS
TTCATCCTCTGCTAAAGCTG+CGG 0.652304 1:+85147140 MsaT005779.1:CDS
TCATCTTTAGAATAATCCGC+CGG 0.653440 1:-85148271 None:intergenic
GAGGGAGCTACATGGTGATG+GGG 0.656552 1:+85151439 MsaT005779.1:CDS
CGTGGTGGGAAGCTTCTAGG+TGG 0.658159 1:+85148477 MsaT005779.1:CDS
TAATTGTTTAGATTGGTGTG+AGG 0.659042 1:+85149106 MsaT005779.1:CDS
TAAATGGTCTGATCATGAAG+GGG 0.660847 1:-85152087 None:intergenic
GCGGCGGCGATAATGAAATG+CGG 0.661761 1:+85147159 MsaT005779.1:CDS
AAAGCTAAATATAAAACCGG+CGG 0.661902 1:+85148255 MsaT005779.1:CDS
TGAGTGCTTCCGTTTGACAG+AGG 0.665002 1:+85153711 MsaT005779.1:CDS
GCCACGTCACCATCCTCCAA+CGG 0.665173 1:-85146928 None:intergenic
AATCCGGCAACTTCACAGCA+AGG 0.666731 1:-85150727 None:intergenic
TAAAGTGGAACTAAAACCTG+AGG 0.669552 1:+85151367 MsaT005779.1:CDS
CGATTGGCTTAAATGGCCTG+AGG 0.670626 1:+85150580 MsaT005779.1:CDS
GGGTGGCATGCAAATGCCGA+TGG 0.671435 1:+85148633 MsaT005779.1:CDS
GCTGGGAACCACTAGTCATG+TGG 0.677762 1:+85149024 MsaT005779.1:CDS
ATGAGTCTACGATTCGTGAG+TGG 0.678627 1:+85148601 MsaT005779.1:CDS
ACGCGGCAGAAAGAGAAAGG+CGG 0.678843 1:+85147335 MsaT005779.1:CDS
TGGGTTCATCTGGATCAGGG+AGG 0.685323 1:-85146846 None:intergenic
TCAATGTCAAAGAAATGACA+GGG 0.687101 1:+85147555 MsaT005779.1:CDS
TTTACTCAAAATCTCGGACG+CGG 0.689301 1:+85150797 MsaT005779.1:CDS
ATGAACTCAGCATGATGACG+AGG 0.692646 1:-85150653 None:intergenic
ATTGTGTAAAGCCTGACATG+GGG 0.693819 1:+85150750 MsaT005779.1:CDS
CTGCATCAGACACATCACAA+TGG 0.698372 1:-85150838 None:intergenic
GGTCGCAATTATCTACATGG+TGG 0.698999 1:+85148534 MsaT005779.1:CDS
GGCTGAAACCAACAACGAGG+AGG 0.705772 1:+85148037 MsaT005779.1:CDS
TGGTGACCTGATGGTTGGAG+AGG 0.707195 1:+85151502 MsaT005779.1:CDS
ATGATATACACAATAACACA+AGG 0.707929 1:+85148847 MsaT005779.1:CDS
GGACGATGAACGTGATGACG+TGG 0.712626 1:+85146990 MsaT005779.1:CDS
AAATGGTCTGATCATGAAGG+GGG 0.713662 1:-85152086 None:intergenic
GCATGTTGGCGCCAAAACGG+TGG 0.714446 1:+85147059 MsaT005779.1:CDS
AGGGCTGTAGATCTACACCA+TGG 0.722496 1:+85148918 MsaT005779.1:CDS
CAACTGGTGAGAAAGAGACG+CGG 0.745058 1:+85147318 MsaT005779.1:CDS
GATAAATCACAGATCCGCTG+CGG 0.750646 1:+85153750 MsaT005779.1:CDS
CGATGAACGTGATGACGTGG+TGG 0.763971 1:+85146993 MsaT005779.1:CDS
TCAATGGCACTGGAGCAAAG+GGG 0.769506 1:+85148956 MsaT005779.1:CDS
GTGAGTGGTCAAGATCTGGG+TGG 0.773278 1:+85148616 MsaT005779.1:CDS
TAAGGCTGAAACCAACAACG+AGG 0.802053 1:+85148034 MsaT005779.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 85146784 85154681 85146784 ID=MsaG005779
Chr1 mRNA 85146784 85154681 85146784 ID=MsaT005779.1;Parent=MsaG005779
Chr1 exon 85146784 85147428 85146784 ID=MsaT005779.1.exon1;Parent=MsaT005779.1
Chr1 CDS 85146784 85147428 85146784 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Chr1 exon 85147526 85147641 85147526 ID=MsaT005779.1.exon2;Parent=MsaT005779.1
Chr1 CDS 85147526 85147641 85147526 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Chr1 exon 85147837 85147936 85147837 ID=MsaT005779.1.exon3;Parent=MsaT005779.1
Chr1 CDS 85147837 85147936 85147837 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Chr1 exon 85148023 85148161 85148023 ID=MsaT005779.1.exon4;Parent=MsaT005779.1
Chr1 CDS 85148023 85148161 85148023 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Chr1 exon 85148242 85148305 85148242 ID=MsaT005779.1.exon5;Parent=MsaT005779.1
Chr1 CDS 85148242 85148305 85148242 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Chr1 exon 85148389 85149127 85148389 ID=MsaT005779.1.exon6;Parent=MsaT005779.1
Chr1 CDS 85148389 85149127 85148389 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Chr1 exon 85150530 85150859 85150530 ID=MsaT005779.1.exon7;Parent=MsaT005779.1
Chr1 CDS 85150530 85150859 85150530 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Chr1 exon 85151344 85151643 85151344 ID=MsaT005779.1.exon8;Parent=MsaT005779.1
Chr1 CDS 85151344 85151643 85151344 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Chr1 exon 85152078 85152150 85152078 ID=MsaT005779.1.exon9;Parent=MsaT005779.1
Chr1 CDS 85152078 85152150 85152078 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Chr1 exon 85152250 85152335 85152250 ID=MsaT005779.1.exon10;Parent=MsaT005779.1
Chr1 CDS 85152250 85152335 85152250 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Chr1 exon 85153664 85153783 85153664 ID=MsaT005779.1.exon11;Parent=MsaT005779.1
Chr1 CDS 85153664 85153783 85153664 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Chr1 exon 85154380 85154438 85154380 ID=MsaT005779.1.exon12;Parent=MsaT005779.1
Chr1 CDS 85154380 85154438 85154380 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Chr1 exon 85154654 85154681 85154654 ID=MsaT005779.1.exon13;Parent=MsaT005779.1
Chr1 CDS 85154654 85154681 85154654 ID=cds.MsaT005779.1;Parent=MsaT005779.1
Gene Sequence

>MsaT005779.1

ATGGCGGAGGAAGAGAACAACGACATAGTCACATATATTGTGAAAATCAAAGTCACACTCACCCTCCCTGATCCAGATGAACCCATTCCTGATCATGATCATCGAGAAACACAACCTACGGCCAATGTTGGCGCCGAAACGGTACCGTTGGAGGATGGTGACGTGGCACAAGCTATCGGTGTTGGAGCTGAAACGTCGTCGTTGAAGGACGATGAACGTGATGACGTGGTGGAAGCAGCTGAAGAATCTATTCCTGATCGAGAAAAACAAGATATGCATGTTGGCGCCAAAACGGTGGTGTCGCTGTCGAAGGACGATGAAGGTTCGGTTGAAGGTGATAACATTGCAGCTAGGGATTCATCCTCTGCTAAAGCTGCGGCGGCGATAATGAAATGCGGCAGAAAGAAAAAGAAACGTGGAAGAAAGAGTTTTGCAGAAATTGAAAAGATGAAGAGGGATGCAGAAGCGTTGTTAGGGAACAGAATAAAGGAAGAAACTGGTAAAGATGTGGTAGCTGGAGGCTCTTCATCATTTCAACTGGTGAGAAAGAGACGCGGCAGAAAGAGAAAGGCGGAGAAAGAAACCACCAAAAATGAGGTTCCACAAGTCCACAAGAAACTTAAAATCAGCGAGAGTAATGCTAAGTGTTTTGAAGGGGTTTCCTCAATGTGCCATCAATGTCAAAGAAATGACAGGGCCGGCGTTGTTAGATGCACAAAATGCAAACGGAAAAGATTTTGCACATCATGTATACAAAAGTGGTATCCTAAATTGAAAGAGAGTGATATTGCCAAGGCATGCCCTGTGTGCTGTGATAATTGCAATTGCAAAGCATGCTTGCGGTCTTTTAAACTCATCAATGAAATGAAGCGTAAGGCTGAAACCAACAACGAGGAGGAGGTTGAGTTCTCTAAGTATCTGCTGAAAGGACTTCTTCCACATTTAAGACGGTTGGATGAAGAACAAATGATTGAGAAGGAAAGAGAAGCCAGGAGACGAGGGCTCTCACTCTCAAAGCTAAATATAAAACCGGCGGATTATTCTAAAGATGAGCGTGTGTTCTGTGACAACTGTAAAACATCAATATTTGACTACCACAGAAGCTGTACAAAATGCTCTTTTGACCTTTGTCTCCTCTGTTGCTGCGAGCTCCGTGGTGGGAAGCTTCTAGGTGGTGCAGATCCAATTAAGCTTGAGTATGATTTTCGAGGTCGCAATTATCTACATGGTGGAAATGAAGAAAAACATGTTAAAGAAAGTGTATCACATGCAGAGGATGAGTCTACGATTCGTGAGTGGTCAAGATCTGGGTGGCATGCAAATGCCGATGGTAGCATTCCTTGTCCAAAAGCTGATAATGAATGTGATCATGGTTTCCTTGAACTGAGAAGGATACTTCCTCCAAATTGCATCTCTGAGTTAGTTTGTAAAGCAAACAAACTTGCAGAAACAATCAAGCTTCAAGATGTAGAGGAGACTCGTGATAATAGGTGTTCTTGCTCGAAACCTGTTAGGTACACCGATGATATACACAATAACACAAGGAAGGCAGCTTTTCATGAAGATTCAAGTGACAAATTTTTATACTGTCCAAGGGCTGTAGATCTACACCATGGAGATTTAAAACATTTTCAATGGCACTGGAGCAAAGGGGAGCCTGTAATTGTCAGCAATGTACTTGAATGCACATCTGGTTTAAGCTGGGAACCACTAGTCATGTGGCGTGCATTCCGTCAAATAACCAACTCCAAATACGATGTAGTTTTGGTTGTGAAGGCAGTTAATTGTTTAGATTGGTGTGAGGGAGATATTAATATCCACCAATTCTTCACTGGGTACACAAATGGTCGCAGCGATTGGCTTAAATGGCCTGAGGTGTTGAAATTGAAAGATTGGCCTCCTTCTGATTTATTTCAGGAACTTTTACCTCGTCATCATGCTGAGTTCATATCTTCCTTGCCTTATAAAGAATACACAAATCCTTTTAGTGGTTCTCTCAACCTTGCTGTGAAGTTGCCGGATTATTGTGTAAAGCCTGACATGGGGCCAAGAACATATATTGCTTATGGATTTACTCAAAATCTCGGACGCGGTGATTCTGTGACTAAGCTCCATTGTGATGTGTCTGATGCAGTAAATGTGTTGACTCATATCGCTAAAGTGGAACTAAAACCTGAGGAAATTAGTGTCATTAAGAAATTGACACGTAAGCACCTTGAGCAAGATAAGAGGGAGCTACATGGTGATGGGGAGGCGGTTGATATGTTTCACCAACTCTCTGACACTAATGATGGTGACCTGATGGTTGGAGAGGATCCCTTAGAAGGTGCTCTCTGGGATATTTTTCGGAGAGAAGATGTACCCAAATTGAAAGAATATCTAGAGAAGCATTTCAGAGAGTTCAGACATGTCAATTGCATTCCTTTAAAGCAGGTTATTGACCCCCTTCATGATCAGACCATTTATCTGAATATGGAGCATAAGATGAAACTTAAGAAAGAGTATGGAATTGAGCCTTGGACTTTTATTCAGAAGTTGGGAGATGCCGTTTTCATTCCTGCTGGTTTGCCTCACCAAGTCAGAAATCTGAAGTCCTGCATCAAGGTTGCACTTGATTTTGTCTCTCCCGAACATATTGGTGAGTGCTTCCGTTTGACAGAGGAATTCCGCAAGCTTCCGATAAATCACAGATCCGCTGCGGACAAGTTTGAGGTGAAGAAAATTGCCGTACATGCTATGCTTGATGTGGTTGAAAAATTAGAGAAAGCAAGGTCTGGGAAAGCCAAGGTTTGTGCGTAA
Protein sequence

>MsaT005779.1

MAEEENNDIVTYIVKIKVTLTLPDPDEPIPDHDHRETQPTANVGAETVPLEDGDVAQAIGVGAETSSLKDDERDDVVEAAEESIPDREKQDMHVGAKTVVSLSKDDEGSVEGDNIAARDSSSAKAAAAIMKCGRKKKKRGRKSFAEIEKMKRDAEALLGNRIKEETGKDVVAGGSSSFQLVRKRRGRKRKAEKETTKNEVPQVHKKLKISESNAKCFEGVSSMCHQCQRNDRAGVVRCTKCKRKRFCTSCIQKWYPKLKESDIAKACPVCCDNCNCKACLRSFKLINEMKRKAETNNEEEVEFSKYLLKGLLPHLRRLDEEQMIEKEREARRRGLSLSKLNIKPADYSKDERVFCDNCKTSIFDYHRSCTKCSFDLCLLCCCELRGGKLLGGADPIKLEYDFRGRNYLHGGNEEKHVKESVSHAEDESTIREWSRSGWHANADGSIPCPKADNECDHGFLELRRILPPNCISELVCKANKLAETIKLQDVEETRDNRCSCSKPVRYTDDIHNNTRKAAFHEDSSDKFLYCPRAVDLHHGDLKHFQWHWSKGEPVIVSNVLECTSGLSWEPLVMWRAFRQITNSKYDVVLVVKAVNCLDWCEGDINIHQFFTGYTNGRSDWLKWPEVLKLKDWPPSDLFQELLPRHHAEFISSLPYKEYTNPFSGSLNLAVKLPDYCVKPDMGPRTYIAYGFTQNLGRGDSVTKLHCDVSDAVNVLTHIAKVELKPEEISVIKKLTRKHLEQDKRELHGDGEAVDMFHQLSDTNDGDLMVGEDPLEGALWDIFRREDVPKLKEYLEKHFREFRHVNCIPLKQVIDPLHDQTIYLNMEHKMKLKKEYGIEPWTFIQKLGDAVFIPAGLPHQVRNLKSCIKVALDFVSPEHIGECFRLTEEFRKLPINHRSAADKFEVKKIAVHAMLDVVEKLEKARSGKAKVCA*