AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005780 MtrunA17_Chr1g0211691 81.393 1005 96 8 1 974 1 945 0.0 1522
MsaG005780 MtrunA17_Chr1g0211711 72.376 1086 224 15 36 1086 7 1051 0.0 1521
MsaG005780 MtrunA17_Chr1g0183311 71.529 850 182 6 287 1086 43 882 0.0 1207
MsaG005780 MtrunA17_Chr5g0426961 70.221 769 205 6 323 1085 84 834 0.0 1091
MsaG005780 MtrunA17_Chr1g0211661 74.218 671 160 5 357 1026 207 865 0.0 1028
MsaG005780 MtrunA17_Chr1g0211661 61.111 126 43 2 1 120 1 126 1.01e-30 130
MsaG005780 MtrunA17_Chr5g0420361 66.124 676 204 9 438 1095 138 806 0.0 901
MsaG005780 MtrunA17_Chr6g0472441 64.307 664 182 12 438 1079 80 710 0.0 848
MsaG005780 MtrunA17_Chr7g0275771 42.222 945 418 25 237 1091 24 930 0.0 665
MsaG005780 MtrunA17_Chr5g0448301 61.404 570 184 7 432 996 93 631 0.0 665
MsaG005780 MtrunA17_Chr5g0448301 40.678 177 62 8 72 242 4 143 3.77e-12 70.5
MsaG005780 MtrunA17_Chr4g0075261 45.526 760 322 15 364 1083 157 864 0.0 629
MsaG005780 MtrunA17_Chr2g0289121 47.765 716 315 12 375 1083 156 819 0.0 615
MsaG005780 MtrunA17_Chr1g0192011 36.193 746 395 15 375 1087 267 964 4.53e-147 466
MsaG005780 MtrunA17_Chr2g0289031 53.827 405 165 3 681 1083 9 393 3.88e-139 425
MsaG005780 MtrunA17_Chr1g0147021 44.673 535 259 10 369 898 864 1366 1.60e-130 436
MsaG005780 MtrunA17_Chr1g0147021 64.780 159 53 1 939 1094 1551 1709 2.46e-58 220
MsaG005780 MtrunA17_Chr2g0281051 34.375 768 409 16 375 1075 207 946 4.85e-130 420
MsaG005780 MtrunA17_Chr1g0211701 95.690 116 5 0 972 1087 4 119 1.48e-75 246
MsaG005780 MtrunA17_Chr8g0364421 50.000 256 96 4 485 739 5 229 7.91e-70 233
MsaG005780 MtrunA17_Chr6g0469821 85.075 134 20 0 1 134 1 134 1.83e-67 223
MsaG005780 MtrunA17_Chr7g0231061 59.574 188 69 2 734 921 8 188 3.06e-63 213
MsaG005780 MtrunA17_Chr4g0048531 41.806 299 159 6 596 888 727 1016 3.15e-62 232
MsaG005780 MtrunA17_Chr4g0048531 51.701 147 70 1 938 1084 1132 1277 1.29e-43 173
MsaG005780 MtrunA17_Chr4g0048531 36.585 164 97 3 375 532 260 422 3.02e-26 117
MsaG005780 MtrunA17_Chr8g0361381 55.080 187 80 2 676 862 26 208 6.61e-60 204
MsaG005780 MtrunA17_Chr8g0340381 65.476 168 39 3 775 942 36 184 2.00e-55 190
MsaG005780 MtrunA17_Chr2g0289091 72.973 111 30 0 938 1048 310 420 1.83e-49 181
MsaG005780 MtrunA17_Chr2g0289091 59.441 143 57 1 753 895 22 163 1.81e-47 176
MsaG005780 MtrunA17_Chr8g0361391 64.545 110 38 1 917 1026 29 137 9.82e-42 150
MsaG005780 MtrunA17_Chr2g0289041 39.070 215 113 7 397 605 2 204 1.06e-38 143
MsaG005780 MtrunA17_Chr8g0364411 57.143 112 48 0 375 486 33 144 3.87e-36 134
MsaG005780 MtrunA17_Chr1g0211681 81.395 86 16 0 49 134 1 86 1.66e-34 127
MsaG005780 MtrunA17_Chr1g0211681 78.788 66 13 1 135 200 28 92 3.37e-24 97.8
MsaG005780 MtrunA17_Chr1g0211671 76.190 63 15 0 1025 1087 15 77 1.27e-27 108
MsaG005780 MtrunA17_Chr3g0119761 66.234 77 26 0 848 924 55 131 8.27e-25 100
MsaG005780 MtrunA17_Chr2g0289101 37.879 132 67 6 507 632 8 130 7.64e-15 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005780 AT1G11950.1 49.309 724 320 11 371 1087 189 872 0.0 686
MsaG005780 AT1G11950.3 49.309 724 320 11 371 1087 189 872 0.0 686
MsaG005780 AT1G62310.1 49.220 705 323 13 374 1072 208 883 0.0 672
MsaG005780 AT4G00990.1 43.135 772 352 14 375 1082 80 828 0.0 593
MsaG005780 AT1G11950.2 48.086 653 292 12 371 1016 189 801 0.0 588
MsaG005780 AT3G07610.1 57.582 488 186 4 588 1075 417 883 0.0 574
MsaG005780 AT3G07610.1 39.556 225 133 2 345 569 127 348 2.27e-46 181
MsaG005780 AT3G07610.3 54.832 507 192 5 588 1075 417 905 2.66e-179 553
MsaG005780 AT3G07610.3 39.556 225 133 2 345 569 127 348 2.05e-46 182
MsaG005780 AT4G00990.3 42.120 717 328 14 375 1027 80 773 1.46e-171 524
MsaG005780 AT4G00990.2 42.297 714 325 14 375 1024 80 770 2.30e-171 523
MsaG005780 AT1G09060.4 33.288 742 429 17 368 1087 211 908 8.11e-119 389
MsaG005780 AT1G09060.3 33.288 742 429 17 368 1087 211 908 8.11e-119 389
MsaG005780 AT1G09060.2 33.288 742 429 17 368 1087 197 894 1.61e-118 388
MsaG005780 AT1G09060.1 33.288 742 429 17 368 1087 197 894 1.61e-118 388
MsaG005780 AT3G07610.2 56.940 281 114 2 588 868 417 690 1.80e-96 324
MsaG005780 AT3G07610.2 39.556 225 133 2 345 569 127 348 2.96e-46 179
MsaG005780 AT4G21430.1 33.333 498 290 7 614 1082 427 911 3.93e-83 291
MsaG005780 AT4G21430.1 29.747 158 108 3 369 524 221 377 1.96e-12 72.4

Find 235 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATAAAGGTTATTCTTTA+AGG 0.076090 1:+85164954 MsaT005780.1:CDS
GGCCTCCTTCTAATTTATTT+AGG 0.135585 1:+85168171 MsaT005780.1:CDS
TGTCTTGCTCAAGGTGCTTT+TGG 0.146606 1:-85168893 None:intergenic
TTGCTCGTCTGAAGCATTTA+TGG 0.202791 1:-85168985 None:intergenic
ATTGATCAGTGAAAAGAATT+TGG 0.212830 1:+85164910 MsaT005780.1:CDS
GCAAAATAGTGTTCGAGTTT+TGG 0.215510 1:+85169004 MsaT005780.1:CDS
TCATCCAATCGTCTTAAATA+TGG 0.224411 1:-85165860 None:intergenic
GTCGCCCAGATTGGCTTAAT+TGG 0.224414 1:+85168123 MsaT005780.1:CDS
TTTAAATATTCCTCCAATTC+AGG 0.228132 1:-85169086 None:intergenic
GCCTCCTTCTAATTTATTTA+GGG 0.243943 1:+85168172 MsaT005780.1:CDS
CTTCGTTGTGGGAAGCTTCT+AGG 0.247218 1:+85166406 MsaT005780.1:CDS
CACCCTCTGATGGCACCGTC+TGG 0.259372 1:-85158784 None:intergenic
GCTTGATGTGGTTAACAAAT+TGG 0.261966 1:+85172878 MsaT005780.1:CDS
AATGTGCACCAATTCTTTAC+TGG 0.265399 1:+85168089 MsaT005780.1:CDS
CCACAATCATCATCGGCTTT+TGG 0.278197 1:-85166598 None:intergenic
CTGACATTGATGGCACATTA+AGG 0.293064 1:-85165189 None:intergenic
AGTACATTGCTGATAATTAC+AGG 0.293307 1:-85166910 None:intergenic
GGATCTGTATATTCTTTATA+CGG 0.299317 1:-85168236 None:intergenic
TACCAAGCACAAAACACTTT+TGG 0.300838 1:+85166999 MsaT005780.1:CDS
ATTCTTTAGATGGTGCTCTC+TGG 0.323154 1:+85169039 MsaT005780.1:CDS
GCATACCTTGTTTAAACAAT+TGG 0.324245 1:+85165267 MsaT005780.1:CDS
CGACAACAACACCACCGTTT+TGG 0.332690 1:-85158936 None:intergenic
AGAAGCATTTCAGAGAGTTT+AGG 0.334304 1:+85169108 MsaT005780.1:CDS
TGTCATTTCTCTGACATTGA+TGG 0.335478 1:-85165199 None:intergenic
GGAAGATTACGTGTCAGATT+TGG 0.338618 1:+85164670 MsaT005780.1:CDS
TTGGTGGACCTGTGGTTTAT+CGG 0.340425 1:-85171916 None:intergenic
CAAGGCCGTGATTATCTTCA+TGG 0.341964 1:+85166463 MsaT005780.1:CDS
AGAAGAAACCTGGTAGAAAA+AGG 0.348376 1:+85164804 MsaT005780.1:CDS
AATAGGGGTGATTCTTTAGA+TGG 0.356154 1:+85169029 MsaT005780.1:CDS
ATATCAGTTGCATTCCTAAC+AGG 0.357202 1:-85166760 None:intergenic
ACTAACTCAGAGATGCAATT+TGG 0.359225 1:-85166655 None:intergenic
TGTGTTTCTCGATCATCCTC+TGG 0.369544 1:-85158740 None:intergenic
CTCGAACTCATGGTTGTTGT+TGG 0.375822 1:-85165807 None:intergenic
TTTGTGAGTGGTCAAGATTC+GGG 0.378563 1:+85166542 MsaT005780.1:CDS
TTGCAAACTTCCTCTCCCTT+TGG 0.380018 1:+85172919 MsaT005780.1:CDS
AGGAAACTTAAGGAGGAGTA+TGG 0.381395 1:+85171211 MsaT005780.1:CDS
CTTTCCCTAAATAAATTAGA+AGG 0.387657 1:-85168176 None:intergenic
ATGACGTAAATTTAAAGCTC+TGG 0.387898 1:-85166845 None:intergenic
AAGAGGGTGAGGAAAAGAAT+TGG 0.395670 1:+85164564 MsaT005780.1:CDS
TGAGGCCAATTAAGCCAATC+TGG 0.400800 1:-85168128 None:intergenic
ATCTGACTCGAGAGCATAAG+AGG 0.403353 1:+85171191 MsaT005780.1:CDS
TCTCGATCATCCTCTGGGTA+TGG 0.404725 1:-85158734 None:intergenic
GTTCTTGTTTGAAGCCTGTT+AGG 0.405975 1:+85166746 MsaT005780.1:CDS
ATGCTCTCGAGTCAGATAAA+AGG 0.409436 1:-85171185 None:intergenic
TCTGACTTGGTGAGGACAAC+CGG 0.410174 1:-85171404 None:intergenic
AAGATAAACTCAAACTCAAT+TGG 0.413084 1:-85166439 None:intergenic
TTCAATGGCACTGGAGCAAA+GGG 0.413215 1:+85166884 MsaT005780.1:CDS
CTTCCGGTTGTGTGAGAAAG+AGG 0.414317 1:+85164618 MsaT005780.1:CDS
TTCTTTAGATGGTGCTCTCT+GGG 0.419089 1:+85169040 MsaT005780.1:CDS
GAGAGAGGATGTACCTGAAT+TGG 0.421371 1:+85169073 MsaT005780.1:CDS
TATGGATTTGCTCAGGAGCT+TGG 0.422137 1:+85168341 MsaT005780.1:CDS
TTCTTTACTGGGTACACAAA+TGG 0.422160 1:+85168101 MsaT005780.1:CDS
AATGTACTTGAATGTACAAC+TGG 0.424187 1:+85166925 MsaT005780.1:CDS
ATTTGTGAGTGGTCAAGATT+CGG 0.429286 1:+85166541 MsaT005780.1:CDS
TCTTCCATATTTAAGACGAT+TGG 0.429788 1:+85165856 MsaT005780.1:CDS
TTGTGTACCCAGTAAAGAAT+TGG 0.434568 1:-85168097 None:intergenic
AGAGGGTGAGGAAAAGAATT+GGG 0.444632 1:+85164565 MsaT005780.1:CDS
AAGTTCTTTCAGCATATACT+TGG 0.448229 1:-85165834 None:intergenic
AATGTACAACTGGTTTAAGC+TGG 0.448278 1:+85166935 MsaT005780.1:CDS
AGCTGATGACATGGCAGCTA+AGG 0.454453 1:+85164439 MsaT005780.1:CDS
CGCCCAGACGGTGCCATCAG+AGG 0.455101 1:+85158781 MsaT005780.1:CDS
TTGCCTCTTTCTCACACAAC+CGG 0.456045 1:-85164621 None:intergenic
GAAGAAGAAGGTCATAATTG+AGG 0.456146 1:+85164727 MsaT005780.1:CDS
AAGAACTCGAAGAAACAATT+AGG 0.456155 1:+85166689 MsaT005780.1:CDS
AGAGCATAAGAGGAAACTTA+AGG 0.461052 1:+85171201 MsaT005780.1:CDS
CTGCTTTAAAGGAGAGCAAT+GGG 0.464779 1:-85169135 None:intergenic
TGAGAAAGAGGCAAAGGCCT+AGG 0.465093 1:+85164630 MsaT005780.1:CDS
AGATTGGCTTAATTGGCCTC+AGG 0.465478 1:+85168130 MsaT005780.1:CDS
CTCGATCATCCTCTGGGTAT+GGG 0.470146 1:-85158733 None:intergenic
TTGTTGTCGTCGGACGATGA+AGG 0.470589 1:+85158950 MsaT005780.1:CDS
GAGATAGAAGCAAAGAGAAA+AGG 0.470722 1:+85165902 MsaT005780.1:CDS
CATGAAGATAATCACGGCCT+TGG 0.473194 1:-85166462 None:intergenic
TGCTTGTAGGAATCGAGCCT+TGG 0.475187 1:+85171341 MsaT005780.1:intron
ACACAAATGGTCGCCCAGAT+TGG 0.475261 1:+85168114 MsaT005780.1:CDS
GACAGTTTATAGTTCGGAAG+AGG 0.480891 1:+85164547 MsaT005780.1:CDS
TGACGTAAATTTAAAGCTCT+GGG 0.482957 1:-85166844 None:intergenic
GTCAGAGAAATGACAAGGGC+AGG 0.483009 1:+85165207 MsaT005780.1:CDS
ACCACTCACAAATCTCAGGC+TGG 0.483334 1:-85166531 None:intergenic
ACCCTCTGATGGCACCGTCT+GGG 0.484196 1:-85158783 None:intergenic
TATTGCTTATGGATTTGCTC+AGG 0.486399 1:+85168334 MsaT005780.1:CDS
AGGCAATTGATTGCTTAGAT+TGG 0.486412 1:+85167028 MsaT005780.1:CDS
TGTGGTTTATCGGAAGCTTA+CGG 0.487799 1:-85171906 None:intergenic
ACGATTGGATGAAGAACAAA+TGG 0.491089 1:+85165871 MsaT005780.1:CDS
TGAAAACAATGCTGCAAAAG+AGG 0.491260 1:+85159012 MsaT005780.1:CDS
ATGTGCACCAATTCTTTACT+GGG 0.492244 1:+85168090 MsaT005780.1:CDS
AGCTTTAAATTTACGTCATG+AGG 0.498076 1:+85166849 MsaT005780.1:CDS
GCATTTCAAATTTCAATGGC+GGG 0.498530 1:+85158653 None:intergenic
CTCGAGTCAGATAAAAGGTC+TGG 0.498993 1:-85171180 None:intergenic
TAGGAAGATTAAATCGTTGA+AGG 0.499461 1:+85164649 MsaT005780.1:CDS
TTACCTTTAGATTTCTGACT+TGG 0.503046 1:-85171417 None:intergenic
GACCTCCAACCTGTCCTTGG+TGG 0.508939 1:-85171932 None:intergenic
TTCAACGATTTAATCTTCCT+AGG 0.509264 1:-85164647 None:intergenic
GCTTGGTATTTCTTATCTGA+CGG 0.510840 1:-85166984 None:intergenic
ACCTGTCCTTGGTGGACCTG+TGG 0.512572 1:-85171924 None:intergenic
CTTGACCACTCACAAATCTC+AGG 0.514084 1:-85166535 None:intergenic
ACAGACCTCCAACCTGTCCT+TGG 0.514908 1:-85171935 None:intergenic
TTTCAATGGCACTGGAGCAA+AGG 0.515097 1:+85166883 MsaT005780.1:CDS
GTGTTTCTCGATCATCCTCT+GGG 0.515125 1:-85158739 None:intergenic
AGATTACGTGTCAGATTTGG+AGG 0.516385 1:+85164673 MsaT005780.1:CDS
GCCCAGACGGTGCCATCAGA+GGG 0.517858 1:+85158782 MsaT005780.1:CDS
GTTGTTGTGAGCTTCGTTGT+GGG 0.519321 1:+85166395 MsaT005780.1:CDS
GCTCCCTCTTGTCTTGCTCA+AGG 0.519343 1:-85168902 None:intergenic
TACGGAATTCCTCAGTCAAA+CGG 0.525984 1:-85171888 None:intergenic
GAGTTTGAGTTTATCTTCCA+AGG 0.526474 1:+85166445 MsaT005780.1:CDS
AAGACAGAGCAGAAGAAACC+TGG 0.528230 1:+85164794 MsaT005780.1:CDS
TTATAGTTCGGAAGAGGGTG+AGG 0.529955 1:+85164553 MsaT005780.1:CDS
TTGTAGTCATGCATCAAGGT+TGG 0.532619 1:+85171826 MsaT005780.1:intron
GAAGTATGCTTCTCAGTTCG+AGG 0.532683 1:-85166630 None:intergenic
AGCTGATGACATGACAACTA+AGG 0.533429 1:+85158982 MsaT005780.1:CDS
GAAGGTGCTGACGTGGAAGA+AGG 0.533445 1:+85164587 MsaT005780.1:CDS
TATGTATGTTGGCGCCAAAA+CGG 0.533882 1:+85158922 MsaT005780.1:CDS
TATATCAAATTCATAACCAA+AGG 0.537378 1:-85172935 None:intergenic
TTTGAATGTTGGCGCCCAGA+CGG 0.538430 1:+85158769 MsaT005780.1:CDS
CAATGCTGCAAAAGAGGGTG+AGG 0.541200 1:+85159018 MsaT005780.1:CDS
TGTATATTCTTTATACGGCA+AGG 0.541667 1:-85168231 None:intergenic
ATGAAAGAGAAGAAGAGTGC+TGG 0.543182 1:+85159049 MsaT005780.1:CDS
TGCCATGTCATCAGCTTCAA+CGG 0.544773 1:-85164432 None:intergenic
CCATTGCTCTCCTTTAAAGC+AGG 0.546741 1:+85169136 MsaT005780.1:CDS
AACTCAGAGATGCAATTTGG+TGG 0.548310 1:-85166652 None:intergenic
TGAGATGCACAAAATGCAAA+CGG 0.552047 1:+85165234 MsaT005780.1:CDS
ATAAAAGGTCTGGTCGTGGA+TGG 0.552092 1:-85171170 None:intergenic
ACACAACCTACTTTGAATGT+TGG 0.552823 1:+85158758 MsaT005780.1:CDS
CCTGCTTTAAAGGAGAGCAA+TGG 0.552913 1:-85169136 None:intergenic
GCCGATGATGATTGTGGTCA+TGG 0.553086 1:+85166604 MsaT005780.1:CDS
TTGTTGTCGACGGACGATGA+AGG 0.553268 1:+85164407 MsaT005780.1:CDS
ACTTGGAGAGCTCGAACTCA+TGG 0.555503 1:-85165817 None:intergenic
TCACCAAGTCAGAAATCTAA+AGG 0.555814 1:+85171414 MsaT005780.1:CDS
AGAGGATGTACCTGAATTGG+AGG 0.558183 1:+85169076 MsaT005780.1:CDS
AGGTTAAGAATGCCTTTGAA+AGG 0.562396 1:-85168257 None:intergenic
ACCACAGGTCCACCAAGGAC+AGG 0.565099 1:+85171923 MsaT005780.1:CDS
ATTAAAGAGAAGAAGAGAGC+TGG 0.566428 1:+85164509 MsaT005780.1:CDS
GGAATACAAGAAGTTGTCAC+TGG 0.567927 1:-85166821 None:intergenic
TCAGGCTGGGCAGCAGAATG+AGG 0.568737 1:-85166517 None:intergenic
GAATGATGAAGCTGAACAAG+AGG 0.569424 1:+85164760 MsaT005780.1:CDS
TGTTGTTGTGAGCTTCGTTG+TGG 0.569590 1:+85166394 MsaT005780.1:CDS
GCACCTTGAGCAAGACAAGA+GGG 0.570458 1:+85168899 MsaT005780.1:CDS
ATGTACAACTGGTTTAAGCT+GGG 0.570533 1:+85166936 MsaT005780.1:CDS
TGAAGCTGAACAAGAGGAGG+AGG 0.572752 1:+85164766 MsaT005780.1:CDS
TGTCATGTCATCAGCTTCAA+CGG 0.573699 1:-85158975 None:intergenic
GCCACGTCATCACCCTCTGA+TGG 0.573745 1:-85158794 None:intergenic
GTTGTCACTGGAATCTTCAC+GGG 0.575662 1:-85166809 None:intergenic
GAATATACAGATCCTTTCAA+AGG 0.575964 1:+85168245 MsaT005780.1:CDS
ATATCAAATTCATAACCAAA+GGG 0.578315 1:-85172934 None:intergenic
AACGGTGGTGTTGTTGTCGT+CGG 0.578724 1:+85158940 MsaT005780.1:CDS
TAAAAGGTCTGGTCGTGGAT+GGG 0.579331 1:-85171169 None:intergenic
AGTTGTCACTGGAATCTTCA+CGG 0.579890 1:-85166810 None:intergenic
TTGTACCAATTGTTTAAACA+AGG 0.581322 1:-85165272 None:intergenic
ACAGTTTATAGTTCGGAAGA+GGG 0.583088 1:+85164548 MsaT005780.1:CDS
AAGGTATCCTCACTTGAAAG+AGG 0.584254 1:+85165601 MsaT005780.1:intron
TCTTTCAATTTCAAAACCTG+AGG 0.586296 1:-85168146 None:intergenic
CTACATGGTGATAATCGAGA+TGG 0.586913 1:+85168924 MsaT005780.1:CDS
TCAGATAAAAGGTCTGGTCG+TGG 0.587061 1:-85171174 None:intergenic
ACTTGGTGAGGACAACCGGC+AGG 0.588247 1:-85171400 None:intergenic
CCACTCACAAATCTCAGGCT+GGG 0.588333 1:-85166530 None:intergenic
TTTAGATTTCTGACTTGGTG+AGG 0.588372 1:-85171412 None:intergenic
GAGGCCAATTAAGCCAATCT+GGG 0.589315 1:-85168127 None:intergenic
TTTGTCTCACCTGAAAATGT+TGG 0.589435 1:+85171856 MsaT005780.1:CDS
ATCATCCTCTGGGTATGGGA+CGG 0.589645 1:-85158729 None:intergenic
CAAGACAAGAGGGAGCTACA+TGG 0.591607 1:+85168909 MsaT005780.1:CDS
TCCCTAAATAAATTAGAAGG+AGG 0.592028 1:-85168173 None:intergenic
CAATTAACAGTGGATTGAAG+AGG 0.593067 1:+85165163 MsaT005780.1:intron
AGCTTCTGAATAAAAGTCCA+AGG 0.593398 1:-85171358 None:intergenic
GCTGGGAACCGCTTGTCATG+TGG 0.594440 1:+85166953 MsaT005780.1:CDS
TTTGCTCAGGAGCTTGGACG+TGG 0.595460 1:+85168347 MsaT005780.1:CDS
GTCCACCAAGGACAGGTTGG+AGG 0.596001 1:+85171930 MsaT005780.1:CDS
AAATGTTCTAAAGCCAGACA+TGG 0.598652 1:+85168298 MsaT005780.1:CDS
TGAAGAACAAATGGTCGAGA+AGG 0.598904 1:+85165880 MsaT005780.1:CDS
AACGGTGGTGTTGTTGTCGA+CGG 0.600381 1:+85164397 MsaT005780.1:CDS
GCCATCAGAGGGTGATGACG+TGG 0.602381 1:+85158793 MsaT005780.1:CDS
GAATTGGGAAGGTGCTGACG+TGG 0.602384 1:+85164580 MsaT005780.1:CDS
CGATATATGTGACTATGTCA+TGG 0.602828 1:-85158685 None:intergenic
TGGGCGCCAACATTCAAAGT+AGG 0.603891 1:-85158764 None:intergenic
CAGGTCCACCAAGGACAGGT+TGG 0.604718 1:+85171927 MsaT005780.1:CDS
CAATGCTGAACAAGAGTGTG+AGG 0.604968 1:+85164475 MsaT005780.1:CDS
GAAACGTGGTAGGAAGAAGA+AGG 0.606573 1:+85164715 MsaT005780.1:CDS
AAATTCATAACCAAAGGGAG+AGG 0.608230 1:-85172929 None:intergenic
GGTGAGGAAAAGAATTGGGA+AGG 0.609015 1:+85164569 MsaT005780.1:CDS
GGTGCTGACGTGGAAGAAGG+AGG 0.610124 1:+85164590 MsaT005780.1:CDS
ACCATGACCACAATCATCAT+CGG 0.612092 1:-85166605 None:intergenic
ACGTAAATTTAAAGCTCTGG+GGG 0.612630 1:-85166842 None:intergenic
TTACGTGTCAGATTTGGAGG+AGG 0.613999 1:+85164676 MsaT005780.1:CDS
AAATGACGAAGATGAACAAG+AGG 0.614355 1:+85164850 MsaT005780.1:CDS
ACTTCCTCCTCTTTCAAGTG+AGG 0.615486 1:-85165608 None:intergenic
AGCACCTTGAGCAAGACAAG+AGG 0.618569 1:+85168898 MsaT005780.1:CDS
TGATGAAGCTGAACAAGAGG+AGG 0.621390 1:+85164763 MsaT005780.1:CDS
TTCCGTTGAAGCTGATGACA+TGG 0.621539 1:+85164430 MsaT005780.1:CDS
TTCATGGTGGAAAAGAAGAG+AGG 0.621997 1:+85166479 MsaT005780.1:CDS
AATTAGGCTTCAAGATGCAG+AGG 0.623081 1:+85166705 MsaT005780.1:CDS
TTGCAATTACCACAGCACAC+TGG 0.624403 1:-85165644 None:intergenic
TCAGAGAAATGACAAGGGCA+GGG 0.624776 1:+85165208 MsaT005780.1:CDS
TTTCAGGCAACTTCACAGCA+AGG 0.625295 1:-85168277 None:intergenic
TCATGGTGGAAAAGAAGAGA+GGG 0.625856 1:+85166480 MsaT005780.1:CDS
GTTGTGTGAGAAAGAGGCAA+AGG 0.627339 1:+85164624 MsaT005780.1:CDS
TGAAGTGAAATTGAAATCTG+AGG 0.628039 1:+85168845 MsaT005780.1:CDS
AATAAACAAACGGAATCCAA+AGG 0.628262 1:+85165024 MsaT005780.1:CDS
TCGTTGTTGAACGACGGTGA+AGG 0.628406 1:+85158845 MsaT005780.1:CDS
GAAGTGAAATTGAAATCTGA+GGG 0.629648 1:+85168846 MsaT005780.1:CDS
TTCCACCATGAAGATAATCA+CGG 0.630533 1:-85166468 None:intergenic
TTAACAAATTGGAGAAAACA+AGG 0.631124 1:+85172889 MsaT005780.1:CDS
GAAAACAATGCTGCAAAAGA+GGG 0.631519 1:+85159013 MsaT005780.1:CDS
GTAAGCTTCCGATAAACCAC+AGG 0.632121 1:+85171908 MsaT005780.1:CDS
CCAAAAGCCGATGATGATTG+TGG 0.633999 1:+85166598 MsaT005780.1:CDS
GTGCTGACGTGGAAGAAGGA+GGG 0.635869 1:+85164591 MsaT005780.1:CDS
TATACACAATAACACAAGGA+AGG 0.636388 1:+85166780 MsaT005780.1:CDS
TATATATGCTATGCTTGATG+TGG 0.638461 1:+85172866 MsaT005780.1:CDS
GAAAGAGGAGGAAGTTGCTG+AGG 0.638538 1:+85165616 MsaT005780.1:CDS
GGGTGGCATGCAGATAGTGA+TGG 0.640615 1:+85166562 MsaT005780.1:CDS
GTATCCTCACTTGAAAGAGG+AGG 0.641340 1:+85165604 MsaT005780.1:CDS
CGACGGTGAAGGTGATGACG+TGG 0.641361 1:+85158856 MsaT005780.1:CDS
TGACGAAGATGAACAAGAGG+GGG 0.641516 1:+85164853 MsaT005780.1:CDS
CTGATATACACAATAACACA+AGG 0.643123 1:+85166776 MsaT005780.1:CDS
GAGGCATGTCCAGTGTGCTG+TGG 0.646992 1:+85165635 MsaT005780.1:CDS
GACGTAAATTTAAAGCTCTG+GGG 0.647211 1:-85166843 None:intergenic
CCACTGTAATATGTCTGATG+CGG 0.649776 1:+85168388 MsaT005780.1:CDS
CGTTGTGGGAAGCTTCTAGG+TGG 0.657294 1:+85166409 MsaT005780.1:CDS
ATTATCAAACATGTCAACGT+TGG 0.657373 1:-85168952 None:intergenic
AATGTTCTAAAGCCAGACAT+GGG 0.657555 1:+85168299 MsaT005780.1:CDS
TCAATGTCAGAGAAATGACA+AGG 0.658738 1:+85165202 MsaT005780.1:CDS
AATGACGAAGATGAACAAGA+GGG 0.659397 1:+85164851 MsaT005780.1:CDS
GCATAAGAGGAAACTTAAGG+AGG 0.660443 1:+85171204 MsaT005780.1:CDS
GTGAGTGGTCAAGATTCGGG+TGG 0.671679 1:+85166545 MsaT005780.1:CDS
GGCCGTGATTATCTTCATGG+TGG 0.676321 1:+85166466 MsaT005780.1:CDS
CCCAGCCTGAGATTTGTGAG+TGG 0.676512 1:+85166530 MsaT005780.1:CDS
AATGCACGCCACATGACAAG+CGG 0.682465 1:-85166961 None:intergenic
GAAACATCGTTGTTGAACGA+CGG 0.687653 1:+85158839 MsaT005780.1:CDS
TGAGTGCTTCCGTTTGACTG+AGG 0.694932 1:+85171879 MsaT005780.1:CDS
ATGACGAAGATGAACAAGAG+GGG 0.700639 1:+85164852 MsaT005780.1:CDS
TGATTGCTTAGATTGGTGTG+AGG 0.703268 1:+85167035 MsaT005780.1:CDS
ATGAACTCAGCACAATGACG+AGG 0.704061 1:-85168203 None:intergenic
GATAAACCACAGGTCCACCA+AGG 0.718071 1:+85171918 MsaT005780.1:CDS
CAATGTCAGAGAAATGACAA+GGG 0.723230 1:+85165203 MsaT005780.1:CDS
GTATGTTGGCGCCAAAACGG+TGG 0.725230 1:+85158925 MsaT005780.1:CDS
CCGCATCAGACATATTACAG+TGG 0.743850 1:-85168388 None:intergenic
TCAATGGCACTGGAGCAAAG+GGG 0.769506 1:+85166885 MsaT005780.1:CDS
ACTCACCGTCCCATACCCAG+AGG 0.775513 1:+85158724 MsaT005780.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 85158668 85172947 85158668 ID=MsaG005780
Chr1 mRNA 85158668 85172947 85158668 ID=MsaT005780.1;Parent=MsaG005780
Chr1 exon 85158668 85159070 85158668 ID=MsaT005780.1.exon1;Parent=MsaT005780.1
Chr1 CDS 85158668 85159070 85158668 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Chr1 exon 85164351 85165045 85164351 ID=MsaT005780.1.exon2;Parent=MsaT005780.1
Chr1 CDS 85164351 85165045 85164351 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Chr1 exon 85165173 85165288 85165173 ID=MsaT005780.1.exon3;Parent=MsaT005780.1
Chr1 CDS 85165173 85165288 85165173 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Chr1 exon 85165604 85165703 85165604 ID=MsaT005780.1.exon4;Parent=MsaT005780.1
Chr1 CDS 85165604 85165703 85165604 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Chr1 exon 85165785 85165923 85165785 ID=MsaT005780.1.exon5;Parent=MsaT005780.1
Chr1 CDS 85165785 85165923 85165785 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Chr1 exon 85166168 85166231 85166168 ID=MsaT005780.1.exon6;Parent=MsaT005780.1
Chr1 CDS 85166168 85166231 85166168 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Chr1 exon 85166321 85167056 85166321 ID=MsaT005780.1.exon7;Parent=MsaT005780.1
Chr1 CDS 85166321 85167056 85166321 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Chr1 exon 85168080 85168409 85168080 ID=MsaT005780.1.exon8;Parent=MsaT005780.1
Chr1 CDS 85168080 85168409 85168080 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Chr1 exon 85168822 85169157 85168822 ID=MsaT005780.1.exon9;Parent=MsaT005780.1
Chr1 CDS 85168822 85169157 85168822 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Chr1 exon 85171160 85171232 85171160 ID=MsaT005780.1.exon10;Parent=MsaT005780.1
Chr1 CDS 85171160 85171232 85171160 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Chr1 exon 85171350 85171435 85171350 ID=MsaT005780.1.exon11;Parent=MsaT005780.1
Chr1 CDS 85171350 85171435 85171350 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Chr1 exon 85171832 85171951 85171832 ID=MsaT005780.1.exon12;Parent=MsaT005780.1
Chr1 CDS 85171832 85171951 85171832 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Chr1 exon 85172852 85172947 85172852 ID=MsaT005780.1.exon13;Parent=MsaT005780.1
Chr1 CDS 85172852 85172947 85172852 ID=cds.MsaT005780.1;Parent=MsaT005780.1
Gene Sequence

>MsaT005780.1

ATGGCGGGAGAAGAGAACCATGACATAGTCACATATATCGTGAAAATCAAAGTCACACTCACCGTCCCATACCCAGAGGATGATCGAGAAACACAACCTACTTTGAATGTTGGCGCCCAGACGGTGCCATCAGAGGGTGATGACGTGGCAGAAGTTATCGATGTTAGCGATGAAACATCGTTGTTGAACGACGGTGAAGGTGATGACGTGGATGCAGCAGCTGAAGAATCTGTTCGTGATCGAGAAAAACAAGATATGTATGTTGGCGCCAAAACGGTGGTGTTGTTGTCGTCGGACGATGAAGGTTCCGTTGAAGCTGATGACATGACAACTAAGGATTCTGATGAAAACAATGCTGCAAAAGAGGGTGAGGAAAATGTCATGAAAGAGAAGAAGAGTGCTGAATCTATTCCTGATCGAGAAAAACAAGATATGGATGTTGGCGCAAAAACGGTGGTGTTGTTGTCGACGGACGATGAAGGTTCCGTTGAAGCTGATGACATGGCAGCTAAGGATTCTGATGAAAACAATGCTGAACAAGAGTGTGAGGAAAAAAAAGTCATTAAAGAGAAGAAGAGAGCTGGTAGAAAAAGAAAAAAGACAGTTTATAGTTCGGAAGAGGGTGAGGAAAAGAATTGGGAAGGTGCTGACGTGGAAGAAGGAGGGTTTTCTTCCGGTTGTGTGAGAAAGAGGCAAAGGCCTAGGAAGATTAAATCGTTGAAGGAAGATTACGTGTCAGATTTGGAGGAGGATATGAAGAAAAAGTGGAAACGTGGTAGGAAGAAGAAGGTCATAATTGAGGTTTCTGATGAGAATGATGAAGCTGAACAAGAGGAGGAGGATGTGAAGACAGAGCAGAAGAAACCTGGTAGAAAAAGGAAAGTAGGTTATGGTTCTGAAGAAAATGACGAAGATGAACAAGAGGGGGAAAATGGTAAAAATTTTAAGATTGTTGAGAATGAATTGATCAGTGAAAAGAATTTGGAGAGTGATGTTTTATCAGATGATAATAAAGGTTATTCTTTAAGGACTGATAGAAAAGTGAAAGTTAAAAGCATTGAACAGCCGAAGCAAAAAATAAACAAACGGAATCCAAAGTGGATTGAAGAGGAGTCCTTAATGTGCCATCAATGTCAGAGAAATGACAAGGGCAGGGTTGTGAGATGCACAAAATGCAAACGGAAAAGATTTTGCATACCTTGTTTAAACAATTGGTATCCTCACTTGAAAGAGGAGGAAGTTGCTGAGGCATGTCCAGTGTGCTGTGGTAATTGCAATTGCAAAGCATGCTTGCGATCATGTGCACTTATCAAAGAAATTAAAATGAAGACAGAAACCAACAACAACCATGAGTTCGAGCTCTCCAAGTATATGCTGAAAGAACTTCTTCCATATTTAAGACGATTGGATGAAGAACAAATGGTCGAGAAGGAGATAGAAGCAAAGAGAAAAGGGCTCTCACATTCTAAGCTAAAAGTAAAAGCCGCAGATTACCCTAAAAATAAGCGTGTGTACTGTGACAACTGCAAAACGTCAATATTTGACTACCACAGAAGCTGTACAAAATGCTCTTTTAACATTTGTCTTCTTTGTTGTTGTGAGCTTCGTTGTGGGAAGCTTCTAGGTGGTACAGATCCAATTGAGTTTGAGTTTATCTTCCAAGGCCGTGATTATCTTCATGGTGGAAAAGAAGAGAGGGTAAAAAAAAATAAACCTCATTCTGCTGCCCAGCCTGAGATTTGTGAGTGGTCAAGATTCGGGTGGCATGCAGATAGTGATGGTAGCATTCCCTGTCCAAAAGCCGATGATGATTGTGGTCATGGTTTCCTCGAACTGAGAAGCATACTTCCACCAAATTGCATCTCTGAGTTAGTATGTAAAGCGAAAGAACTCGAAGAAACAATTAGGCTTCAAGATGCAGAGGAGACATTTGATAGTATGTGTTCTTGTTTGAAGCCTGTTAGGAATGCAACTGATATACACAATAACACAAGGAAGGCAGCTTCCCGTGAAGATTCCAGTGACAACTTCTTGTATTCCCCCAGAGCTTTAAATTTACGTCATGAGGATTTAAGACATTTTCAATGGCACTGGAGCAAAGGGGAGCCTGTAATTATCAGCAATGTACTTGAATGTACAACTGGTTTAAGCTGGGAACCGCTTGTCATGTGGCGTGCATTCCGTCAGATAAGAAATACCAAGCACAAAACACTTTTGGATGTGGAGGCAATTGATTGCTTAGATTGGTGTGAGGAAAATATTAATGTGCACCAATTCTTTACTGGGTACACAAATGGTCGCCCAGATTGGCTTAATTGGCCTCAGGTTTTGAAATTGAAAGATTGGCCTCCTTCTAATTTATTTAGGGAAAGTTTGCCTCGTCATTGTGCTGAGTTCATATCTTCCTTGCCGTATAAAGAATATACAGATCCTTTCAAAGGCATTCTTAACCTTGCTGTGAAGTTGCCTGAAAATGTTCTAAAGCCAGACATGGGCCCAAAAACATATATTGCTTATGGATTTGCTCAGGAGCTTGGACGTGGTGATTCTGTGACTAAGCTCCACTGTAATATGTCTGATGCGGTAAATGTGTTGACTCATATTGCTGAAGTGAAATTGAAATCTGAGGGCATTGCTGCCATTAAGAAACTGACCCAAAAGCACCTTGAGCAAGACAAGAGGGAGCTACATGGTGATAATCGAGATGGAGAGACCAACGTTGACATGTTTGATAATTCATCTTCTTCCATAAATGCTTCAGACGAGCAAAATAGTGTTCGAGTTTTGGAAAATAGGGGTGATTCTTTAGATGGTGCTCTCTGGGATATTTTTCGGAGAGAGGATGTACCTGAATTGGAGGAATATTTAAAGAAGCATTTCAGAGAGTTTAGGCATGCCCATTGCTCTCCTTTAAAGCAGGTTATTCATCCCATCCACGACCAGACCTTTTATCTGACTCGAGAGCATAAGAGGAAACTTAAGGAGGAGTATGGAATCGAGCCTTGGACTTTTATTCAGAAGCTAGGAGATGCTGTTTTCATTCCTGCCGGTTGTCCTCACCAAGTCAGAAATCTAAAGTCATGCATCAAGGTTGGACTTGATTTTGTCTCACCTGAAAATGTTGGTGAGTGCTTCCGTTTGACTGAGGAATTCCGTAAGCTTCCGATAAACCACAGGTCCACCAAGGACAGGTTGGAGGTGAAGAAAATGACTATATATGCTATGCTTGATGTGGTTAACAAATTGGAGAAAACAAGGTTACTGATTGCAAACTTCCTCTCCCTTTGGTTATGA
Protein sequence

>MsaT005780.1

MAGEENHDIVTYIVKIKVTLTVPYPEDDRETQPTLNVGAQTVPSEGDDVAEVIDVSDETSLLNDGEGDDVDAAAEESVRDREKQDMYVGAKTVVLLSSDDEGSVEADDMTTKDSDENNAAKEGEENVMKEKKSAESIPDREKQDMDVGAKTVVLLSTDDEGSVEADDMAAKDSDENNAEQECEEKKVIKEKKRAGRKRKKTVYSSEEGEEKNWEGADVEEGGFSSGCVRKRQRPRKIKSLKEDYVSDLEEDMKKKWKRGRKKKVIIEVSDENDEAEQEEEDVKTEQKKPGRKRKVGYGSEENDEDEQEGENGKNFKIVENELISEKNLESDVLSDDNKGYSLRTDRKVKVKSIEQPKQKINKRNPKWIEEESLMCHQCQRNDKGRVVRCTKCKRKRFCIPCLNNWYPHLKEEEVAEACPVCCGNCNCKACLRSCALIKEIKMKTETNNNHEFELSKYMLKELLPYLRRLDEEQMVEKEIEAKRKGLSHSKLKVKAADYPKNKRVYCDNCKTSIFDYHRSCTKCSFNICLLCCCELRCGKLLGGTDPIEFEFIFQGRDYLHGGKEERVKKNKPHSAAQPEICEWSRFGWHADSDGSIPCPKADDDCGHGFLELRSILPPNCISELVCKAKELEETIRLQDAEETFDSMCSCLKPVRNATDIHNNTRKAASREDSSDNFLYSPRALNLRHEDLRHFQWHWSKGEPVIISNVLECTTGLSWEPLVMWRAFRQIRNTKHKTLLDVEAIDCLDWCEENINVHQFFTGYTNGRPDWLNWPQVLKLKDWPPSNLFRESLPRHCAEFISSLPYKEYTDPFKGILNLAVKLPENVLKPDMGPKTYIAYGFAQELGRGDSVTKLHCNMSDAVNVLTHIAEVKLKSEGIAAIKKLTQKHLEQDKRELHGDNRDGETNVDMFDNSSSSINASDEQNSVRVLENRGDSLDGALWDIFRREDVPELEEYLKKHFREFRHAHCSPLKQVIHPIHDQTFYLTREHKRKLKEEYGIEPWTFIQKLGDAVFIPAGCPHQVRNLKSCIKVGLDFVSPENVGECFRLTEEFRKLPINHRSTKDRLEVKKMTIYAMLDVVNKLEKTRLLIANFLSLWL*