AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005781

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005781 MtrunA17_Chr1g0211711 87.453 1068 88 7 1 1039 1 1051 0.0 1831
MsaG005781 MtrunA17_Chr1g0183311 78.913 773 118 5 302 1039 120 882 0.0 1221
MsaG005781 MtrunA17_Chr1g0211691 69.990 953 205 14 7 927 42 945 0.0 1205
MsaG005781 MtrunA17_Chr5g0426961 74.274 723 167 4 318 1038 129 834 0.0 1091
MsaG005781 MtrunA17_Chr1g0211661 74.251 668 160 4 313 979 209 865 0.0 1013
MsaG005781 MtrunA17_Chr5g0420361 68.816 667 180 9 385 1032 131 788 0.0 917
MsaG005781 MtrunA17_Chr6g0472441 65.276 671 177 12 385 1032 73 710 0.0 858
MsaG005781 MtrunA17_Chr7g0275771 46.936 816 316 19 325 1044 136 930 0.0 676
MsaG005781 MtrunA17_Chr4g0075261 47.012 753 320 15 318 1036 157 864 0.0 654
MsaG005781 MtrunA17_Chr5g0448301 60.211 568 193 6 383 949 96 631 0.0 646
MsaG005781 MtrunA17_Chr2g0289121 49.440 714 317 11 323 1036 150 819 0.0 643
MsaG005781 MtrunA17_Chr1g0192011 37.467 758 390 16 329 1051 267 975 8.57e-151 475
MsaG005781 MtrunA17_Chr2g0289031 56.543 405 155 3 634 1036 8 393 1.23e-149 451
MsaG005781 MtrunA17_Chr1g0147021 46.828 536 246 11 323 852 864 1366 2.24e-136 451
MsaG005781 MtrunA17_Chr1g0147021 66.879 157 49 1 892 1045 1551 1707 5.88e-60 225
MsaG005781 MtrunA17_Chr2g0281051 34.465 766 428 13 329 1037 207 955 1.04e-134 431
MsaG005781 MtrunA17_Chr1g0211701 84.483 116 18 0 925 1040 4 119 5.76e-67 221
MsaG005781 MtrunA17_Chr4g0048531 42.345 307 162 6 543 843 720 1017 1.07e-65 242
MsaG005781 MtrunA17_Chr4g0048531 46.354 192 94 2 854 1037 1087 1277 1.67e-44 176
MsaG005781 MtrunA17_Chr4g0048531 34.659 176 103 4 329 494 260 433 9.76e-27 118
MsaG005781 MtrunA17_Chr8g0364421 50.584 257 93 5 439 693 5 229 6.23e-65 219
MsaG005781 MtrunA17_Chr7g0231061 60.963 187 67 2 688 874 8 188 3.21e-63 213
MsaG005781 MtrunA17_Chr8g0361381 54.545 187 81 2 630 816 26 208 6.52e-61 207
MsaG005781 MtrunA17_Chr8g0340381 64.881 168 39 4 729 895 36 184 1.86e-53 185
MsaG005781 MtrunA17_Chr2g0289091 68.800 125 39 0 877 1001 296 420 2.86e-51 187
MsaG005781 MtrunA17_Chr2g0289091 60.714 140 55 0 708 847 23 162 2.73e-48 178
MsaG005781 MtrunA17_Chr2g0289041 42.584 209 113 4 351 559 2 203 4.09e-43 155
MsaG005781 MtrunA17_Chr8g0361391 65.455 110 37 1 870 979 29 137 1.44e-42 152
MsaG005781 MtrunA17_Chr8g0364411 60.360 111 44 0 329 439 33 143 1.04e-37 138
MsaG005781 MtrunA17_Chr1g0211671 76.190 63 15 0 978 1040 15 77 6.83e-28 109
MsaG005781 MtrunA17_Chr3g0119761 68.831 77 23 1 802 877 55 131 1.76e-24 100
MsaG005781 MtrunA17_Chr2g0289101 34.646 127 78 4 461 586 8 130 2.12e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005781 AT1G11950.1 50.694 720 315 12 325 1040 189 872 0.0 706
MsaG005781 AT1G11950.3 50.694 720 315 12 325 1040 189 872 0.0 706
MsaG005781 AT1G62310.1 50.568 704 314 13 328 1025 208 883 0.0 688
MsaG005781 AT1G11950.2 48.844 649 292 12 325 969 189 801 0.0 602
MsaG005781 AT4G00990.1 44.459 767 332 16 329 1027 80 820 0.0 596
MsaG005781 AT3G07610.1 57.551 490 186 4 540 1028 415 883 0.0 564
MsaG005781 AT3G07610.1 45.918 196 105 1 328 523 154 348 1.24e-50 195
MsaG005781 AT3G07610.3 54.813 509 192 5 540 1028 415 905 3.46e-176 543
MsaG005781 AT3G07610.3 45.918 196 105 1 328 523 154 348 1.39e-50 195
MsaG005781 AT4G00990.3 42.621 725 322 16 329 985 80 778 7.15e-174 528
MsaG005781 AT4G00990.2 42.957 717 315 16 329 977 80 770 7.13e-173 526
MsaG005781 AT1G09060.2 33.289 751 416 19 322 1040 197 894 1.03e-118 388
MsaG005781 AT1G09060.1 33.289 751 416 19 322 1040 197 894 1.03e-118 388
MsaG005781 AT1G09060.4 33.289 751 416 19 322 1040 211 908 1.41e-118 388
MsaG005781 AT1G09060.3 33.289 751 416 19 322 1040 211 908 1.41e-118 388
MsaG005781 AT3G07610.2 56.184 283 117 2 540 822 415 690 2.59e-92 312
MsaG005781 AT3G07610.2 45.918 196 105 1 328 523 154 348 1.95e-50 192
MsaG005781 AT4G21430.1 33.988 509 306 7 552 1037 412 913 4.25e-85 296
MsaG005781 AT4G21430.1 28.261 184 128 4 297 478 196 377 9.74e-13 73.2

Find 232 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGATCTGTATATTCTTTAT+AGG 0.218933 1:-85197999 None:intergenic
AAGTACTTTCAGCATATATT+TGG 0.244019 1:-85195622 None:intergenic
GTCGCCCTGATTGGCTAAAT+TGG 0.253094 1:+85197885 MsaT005781.1:CDS
TCACCATCACCTTCAGCTTT+TGG 0.263787 1:-85196422 None:intergenic
TGCGTTCCAGTCATGGAAAA+TGG 0.264659 1:+85198844 MsaT005781.1:CDS
AATGTGCACCAATTCTTTAC+TGG 0.265399 1:+85197851 MsaT005781.1:CDS
TCATCCAAACGTCTTAAATA+TGG 0.272167 1:-85195648 None:intergenic
TTTCTACCGCGTTTCTTTGC+CGG 0.274566 1:-85194354 None:intergenic
AACTTCACGCCCTCTGGTTA+TGG 0.274680 1:+85206895 MsaT005781.1:CDS
CCTAAAACATATATTGCTTA+TGG 0.279102 1:+85198085 MsaT005781.1:CDS
ACTGACCAGTGAAAAGAATT+TGG 0.282893 1:+85194824 MsaT005781.1:CDS
CGAGGGCGTGATTATCTTCA+TGG 0.286488 1:+85196287 MsaT005781.1:CDS
TCAGCCTTACTCTTTCTTCC+TGG 0.291739 1:-85194222 None:intergenic
GGATGAGGCTGTGAAGAAAA+AGG 0.294538 1:+85194509 MsaT005781.1:CDS
AGTACATTGCTAACAATTAC+AGG 0.298489 1:-85196734 None:intergenic
GTTGTACCAATTGTTTAAAC+AGG 0.300702 1:-85195186 None:intergenic
TACCAAGCACAAAACACTTT+TGG 0.300838 1:+85196823 MsaT005781.1:CDS
TTTAAATATTCCTCCAACTT+AGG 0.302596 1:-85198919 None:intergenic
AGTTGCAGATATCCCTAAAA+AGG 0.306036 1:+85196031 MsaT005781.1:CDS
TGAAGATTACTTGTCGGATT+TGG 0.306329 1:+85194488 MsaT005781.1:CDS
TGCTGAAACGGTGCCGTTAA+AGG 0.315756 1:+85194032 MsaT005781.1:CDS
GCATACCCTGTTTAAACAAT+TGG 0.324245 1:+85195180 MsaT005781.1:CDS
ATTCCTTAGACGGTGCTCTC+TGG 0.324744 1:+85198872 MsaT005781.1:CDS
GTTCTTGTTTAAAGCCTGTT+AGG 0.328431 1:+85196570 MsaT005781.1:CDS
CTTTCTTCAAATAAGTTAGA+AGG 0.331972 1:-85197938 None:intergenic
GATGAACAAGATGGGGAAAA+TGG 0.335461 1:+85194774 MsaT005781.1:CDS
TGTCATTTCTCTGACATTGA+TGG 0.335478 1:-85195112 None:intergenic
AGGAAGGAAATTTCTAGTTC+TGG 0.339985 1:+85194642 MsaT005781.1:CDS
TCTTCCATATTTAAGACGTT+TGG 0.340249 1:+85195644 MsaT005781.1:CDS
AGGAGGCTTAAGGAGGAGTA+TGG 0.344150 1:+85204797 MsaT005781.1:CDS
GGATCTGTATATTCTTTATA+GGG 0.347461 1:-85197998 None:intergenic
ATCCCAGAGAGCACCGTCTA+AGG 0.348922 1:-85198875 None:intergenic
CTTCGTGGCGGAAAGCTTCT+AGG 0.350575 1:+85196230 MsaT005781.1:CDS
TGATGCTGTTCAATGCATTC+AGG 0.352889 1:-85194909 None:intergenic
ACCGAAAAGGAAAATAAAAC+AGG 0.357268 1:+85194384 MsaT005781.1:CDS
CACCCAGATCTTGACCATTC+AGG 0.357316 1:-85196368 None:intergenic
TATGTAATACCTTCAGATTT+CGG 0.357408 1:-85204990 None:intergenic
AGAAGCATTTCAGAGAGTTC+AGG 0.360974 1:+85198941 MsaT005781.1:CDS
AAACAGCATCTCCTAGCTTC+TGG 0.362555 1:-85204939 None:intergenic
ATATCCTCTTCATTCCTAAC+AGG 0.364490 1:-85196584 None:intergenic
TGTGATTTACCGGAAGTTTA+CGG 0.365363 1:-85206008 None:intergenic
GTCCTTCAACGACGCCGTTT+CGG 0.366610 1:-85194109 None:intergenic
AGTAACTCAGAGATGCAATT+TGG 0.373196 1:-85196479 None:intergenic
GTTCAACAAGAGAAATTCTA+AGG 0.374892 1:+85194944 MsaT005781.1:CDS
AGTGATGAAGCTGAACATAA+AGG 0.377667 1:+85194579 MsaT005781.1:CDS
GTGATGAAGCTGAACATAAA+GGG 0.380882 1:+85194580 MsaT005781.1:CDS
TAACCTTGCTGTGAAGTTGC+CGG 0.383168 1:+85198036 MsaT005781.1:CDS
TGAGGCCAATTTAGCCAATC+AGG 0.389236 1:-85197890 None:intergenic
AAGATAAACTCAAACTCAAT+TGG 0.397071 1:-85196263 None:intergenic
GTTGGAGGAAGCAAGGTTAG+TGG 0.397522 1:+85206867 MsaT005781.1:CDS
TGATAAGAAAATACGGTAGA+AGG 0.397866 1:+85194190 MsaT005781.1:CDS
ATTCCTGAATGGTCAAGATC+TGG 0.400760 1:+85196365 MsaT005781.1:CDS
GCGTTCCAGTCATGGAAAAT+GGG 0.405689 1:+85198845 MsaT005781.1:CDS
GGAGCTACATGGCGATAATC+AGG 0.407696 1:+85198756 MsaT005781.1:CDS
TCTGTGGACCTGTGATTTAC+CGG 0.411222 1:-85206018 None:intergenic
TATGGATTTGAGCAGGAACT+TGG 0.416922 1:+85198103 MsaT005781.1:CDS
TTGTGTACCCAGTAAAGAAT+TGG 0.418147 1:-85197859 None:intergenic
ATTGAATGAAGATTACTTGT+CGG 0.418626 1:+85194482 MsaT005781.1:CDS
GCCACGTCATCAACCTTTAA+CGG 0.420342 1:-85194045 None:intergenic
AATGTACTTGAATGTACATC+TGG 0.421952 1:+85196749 MsaT005781.1:CDS
CTCAACGTCATGGTCGTTGT+TGG 0.423084 1:-85195595 None:intergenic
ACACAAAAGGTCGCCCTGAT+TGG 0.425060 1:+85197876 MsaT005781.1:CDS
CGAGCATAAGAGGAGGCTTA+AGG 0.430431 1:+85204787 MsaT005781.1:CDS
ATACCTTCAGATTTCGGACT+TGG 0.436051 1:-85204984 None:intergenic
CACAAAATCAAGAGCAACCT+TGG 0.439982 1:-85205941 None:intergenic
TTCAACGTCACTGGAGCAAA+GGG 0.444849 1:+85196708 MsaT005781.1:CDS
GACGATTTAGCTTCTGATGA+AGG 0.445346 1:+85194129 MsaT005781.1:CDS
AATGTACATCTGGTTTAAGC+TGG 0.447849 1:+85196759 MsaT005781.1:CDS
ATGAACGGAAATAGAGTTTC+CGG 0.450390 1:-85194325 None:intergenic
ATTTGACTATCGAGCATAAG+AGG 0.450485 1:+85204777 MsaT005781.1:CDS
TTCTTTACTGGGTACACAAA+AGG 0.450970 1:+85197863 MsaT005781.1:CDS
TCAAATAAAAGTGCTGATCA+TGG 0.451104 1:-85204760 None:intergenic
CAGGTCCACAGAGGACAAAT+TGG 0.454737 1:+85206029 MsaT005781.1:CDS
AAGCTATTGATTGCTTAGAT+TGG 0.456101 1:+85196852 MsaT005781.1:CDS
GCTTGATCTGGTGGAGAAGT+TGG 0.461045 1:+85206849 MsaT005781.1:CDS
TATATATGCTATGCTTGATC+TGG 0.462454 1:+85206837 MsaT005781.1:CDS
GAGAGAGGATGTTCCTAAGT+TGG 0.464977 1:+85198906 MsaT005781.1:CDS
TGATTGGCTAAATTGGCCTC+AGG 0.470753 1:+85197892 MsaT005781.1:CDS
CTTTGTTGCTGTGAGCTTCG+TGG 0.470782 1:+85196215 MsaT005781.1:CDS
TTCCTTAGACGGTGCTCTCT+GGG 0.471924 1:+85198873 MsaT005781.1:CDS
CTCCGAAACGGCGTCGTTGA+AGG 0.474138 1:+85194107 MsaT005781.1:CDS
GTTGCAAACTTCACGCCCTC+TGG 0.475965 1:+85206889 MsaT005781.1:CDS
AGAATAACCTTTAATATCAT+CGG 0.477151 1:-85194862 None:intergenic
TTTAACGGAAGAAGAATCTT+CGG 0.478230 1:-85194166 None:intergenic
AAGGTGCTGATGTGGTAGGA+GGG 0.479079 1:+85194409 MsaT005781.1:CDS
TTCAATGATTTAATCTTCCT+AGG 0.480160 1:-85194465 None:intergenic
AGAAGAAACCTGGTAGGAAA+AGG 0.480370 1:+85194622 MsaT005781.1:CDS
TGAGGAAGAGTGAAAGGCCT+AGG 0.480507 1:+85194448 MsaT005781.1:CDS
AATGATGAAGATGAACAAGA+TGG 0.481647 1:+85194765 MsaT005781.1:CDS
ATGATGAAGATGAACAAGAT+GGG 0.482973 1:+85194766 MsaT005781.1:CDS
GTCAGAGAAATGACAAGGGC+AGG 0.483009 1:+85195120 MsaT005781.1:CDS
GAAGGTGCTGATGTGGTAGG+AGG 0.485577 1:+85194408 MsaT005781.1:CDS
TCTTGACCATTCAGGAATCT+CGG 0.486716 1:-85196360 None:intergenic
GAAGTGTGCTTCTCAGTTCA+AGG 0.488983 1:-85196454 None:intergenic
AAACAACACTGGTGCTGAAA+CGG 0.491743 1:+85194020 MsaT005781.1:CDS
ATGTGCACCAATTCTTTACT+GGG 0.492244 1:+85197852 MsaT005781.1:CDS
ATTTGAGCAGGAACTTGGGC+GGG 0.495309 1:+85198108 MsaT005781.1:CDS
ACGTTTGGATGAAGAGCAAA+TGG 0.496696 1:+85195659 MsaT005781.1:CDS
TGAAGAGCAAATGGCTGAGA+AGG 0.497473 1:+85195668 MsaT005781.1:CDS
ACAGTATAAAATGTTATCAC+TGG 0.497517 1:-85196645 None:intergenic
GAAACCAGGAAGAAAGAGTA+AGG 0.501881 1:+85194218 MsaT005781.1:CDS
TATTGCTTATGGATTTGAGC+AGG 0.503016 1:+85198096 MsaT005781.1:CDS
CTTGACCATTCAGGAATCTC+GGG 0.507043 1:-85196359 None:intergenic
AGAGGAATTCCGTAAACTTC+CGG 0.508664 1:+85205999 MsaT005781.1:CDS
TGTATATTCTTTATAGGGCA+AGG 0.509994 1:-85197993 None:intergenic
TTACTTGTCGGATTTGGATG+AGG 0.510978 1:+85194494 MsaT005781.1:CDS
GTTAAAGTGATAAGAAAATA+CGG 0.512225 1:+85194183 MsaT005781.1:CDS
TTATGTAGATTTACAGCCCT+GGG 0.513523 1:-85196668 None:intergenic
AGGAAGAAGAAGGTCATAAT+CGG 0.513842 1:+85194543 MsaT005781.1:CDS
CTGACATTGATGGCACATCA+AGG 0.514049 1:-85195102 None:intergenic
ACCATTCAGGAATCTCGGGC+AGG 0.514330 1:-85196355 None:intergenic
GCGATCATCTGTACTTATCA+AGG 0.516406 1:+85195470 MsaT005781.1:CDS
GGCTGTAAATCTACATAATG+AGG 0.517325 1:+85196673 MsaT005781.1:CDS
TTTCAACGTCACTGGAGCAA+AGG 0.524189 1:+85196707 MsaT005781.1:CDS
TGGTAGAACTGTTAAGATTG+AGG 0.524235 1:+85194794 MsaT005781.1:CDS
TAAAGGTTATTCTTTAAGAA+CGG 0.524469 1:+85194872 MsaT005781.1:CDS
TTTGAGCAGGAACTTGGGCG+GGG 0.525966 1:+85198109 MsaT005781.1:CDS
TGAATTATCAAGCATGATAG+TGG 0.526520 1:-85198788 None:intergenic
CTTTGCCGGAGTGGCATGAA+CGG 0.528885 1:-85194340 None:intergenic
ATATGCTATGCTTGATCTGG+TGG 0.530201 1:+85206840 MsaT005781.1:CDS
GACTTGTAGGAATTGAACCT+TGG 0.533351 1:+85204908 MsaT005781.1:intron
TCTGGCTTTAGAACATCATC+CGG 0.535804 1:-85198055 None:intergenic
ATTATGTAGATTTACAGCCC+TGG 0.540030 1:-85196669 None:intergenic
ACTTGGTGCGGACAACCAGC+TGG 0.541187 1:-85204967 None:intergenic
TTCCTGAATGGTCAAGATCT+GGG 0.542203 1:+85196366 MsaT005781.1:CDS
ATAAAAGTGCTGATCATGGA+TGG 0.542431 1:-85204756 None:intergenic
GCACCGTGAGCAAGACAAGA+GGG 0.549677 1:+85198735 MsaT005781.1:CDS
AACTCAGAGATGCAATTTGG+TGG 0.552022 1:-85196476 None:intergenic
TCAGATGCACAAAATGCAAA+CGG 0.552047 1:+85195147 MsaT005781.1:CDS
GCTGAAGGTGATGGTGATCA+TGG 0.557225 1:+85196428 MsaT005781.1:CDS
GGTGATGATGACATGGCAGC+CGG 0.559212 1:+85194306 MsaT005781.1:CDS
AAGGTATCCTCACTTGAAAG+AGG 0.562825 1:+85195389 MsaT005781.1:intron
TATTTCCGTTCATGCCACTC+CGG 0.564491 1:+85194335 MsaT005781.1:CDS
TACCTCCAATTTGTCCTCTG+TGG 0.565315 1:-85206034 None:intergenic
ACGGAGGAAGCGTTTGAGAA+CGG 0.565695 1:+85194261 MsaT005781.1:CDS
GGGGAAGGTGCTGATGTGGT+AGG 0.566654 1:+85194405 MsaT005781.1:CDS
ATGTACATCTGGTTTAAGCT+GGG 0.567172 1:+85196760 MsaT005781.1:CDS
ATGGATTTGAGCAGGAACTT+GGG 0.568762 1:+85198104 MsaT005781.1:CDS
TTCAGATTTCGGACTTGGTG+CGG 0.569534 1:-85204979 None:intergenic
GCTCCCTCTTGTCTTGCTCA+CGG 0.570052 1:-85198738 None:intergenic
GATTTGAGCAGGAACTTGGG+CGG 0.570180 1:+85198107 MsaT005781.1:CDS
ACCGCGTTTCTTTGCCGGAG+TGG 0.570477 1:-85194349 None:intergenic
CCCTGCCCGAGATTCCTGAA+TGG 0.574410 1:+85196354 MsaT005781.1:CDS
GTAAACTTCCGGTAAATCAC+AGG 0.577040 1:+85206010 MsaT005781.1:CDS
ACAAGCTATTGTCTCCGAAA+CGG 0.577131 1:+85194095 MsaT005781.1:CDS
GAGTTTGAGTTTATCTTCCG+AGG 0.578175 1:+85196269 MsaT005781.1:CDS
CCACTGCTCTCCTTTAAAGC+AGG 0.579600 1:+85198969 MsaT005781.1:CDS
CACGAAGCTCACAGCAACAA+AGG 0.579879 1:-85196214 None:intergenic
GAATATACAGATCCTTTCAA+AGG 0.581672 1:+85198007 MsaT005781.1:CDS
AGGTTAAGAACACCTTTGAA+AGG 0.581830 1:-85198019 None:intergenic
CAAGACAAGAGGGAGCTACA+TGG 0.584081 1:+85198745 MsaT005781.1:CDS
CGTTCCAGTCATGGAAAATG+GGG 0.586219 1:+85198846 MsaT005781.1:CDS
TCTTTCAATTTCAAAACCTG+AGG 0.586296 1:-85197908 None:intergenic
AACAGGGGAAGGTGCTGATG+TGG 0.587266 1:+85194401 MsaT005781.1:CDS
AAGGAAAATAAAACAGGGGA+AGG 0.587449 1:+85194390 MsaT005781.1:CDS
ATGCATTGAACAGCATCAAA+CGG 0.587455 1:+85194914 MsaT005781.1:CDS
TCTTCAAATAAGTTAGAAGG+AGG 0.588526 1:-85197935 None:intergenic
TGAAATCGAGAAGGAGAAGA+CGG 0.589053 1:+85194242 MsaT005781.1:CDS
CCGAAAAGGAAAATAAAACA+GGG 0.590176 1:+85194385 MsaT005781.1:CDS
GCTTGGTATTGCTTATCTGA+CGG 0.590810 1:-85196808 None:intergenic
TTGGCGTGAGGAAGAGTGAA+AGG 0.593194 1:+85194442 MsaT005781.1:CDS
GCTGGGAACCGCTTGTCATG+TGG 0.594440 1:+85196777 MsaT005781.1:CDS
AGTTTGAGTTTATCTTCCGA+GGG 0.594469 1:+85196270 MsaT005781.1:CDS
CAGAGAAGAAGAAACCTGGT+AGG 0.597254 1:+85194616 MsaT005781.1:CDS
AAGACAGAGAAGAAGAAACC+TGG 0.600967 1:+85194612 MsaT005781.1:CDS
AGCACCGTGAGCAAGACAAG+AGG 0.602066 1:+85198734 MsaT005781.1:CDS
TGATGAAGATGAACAAGATG+GGG 0.602700 1:+85194767 MsaT005781.1:CDS
TGATGTTCTAAAGCCAGACA+TGG 0.603316 1:+85198060 MsaT005781.1:CDS
TAAAAGTGCTGATCATGGAT+GGG 0.604516 1:-85204755 None:intergenic
CGAGTGCTTTCGTTTGACAG+AGG 0.605461 1:+85205981 MsaT005781.1:CDS
GAAACGTGGTAGGAAGAAGA+AGG 0.606573 1:+85194533 MsaT005781.1:CDS
TGATGAAGCTGAACATAAAG+GGG 0.606979 1:+85194581 MsaT005781.1:CDS
GAAACCTGGTAGGAAAAGGA+AGG 0.610046 1:+85194626 MsaT005781.1:CDS
GTCCACAGAGGACAAATTGG+AGG 0.610429 1:+85206032 MsaT005781.1:CDS
AAAGCCTGTTAGGAATGAAG+AGG 0.611246 1:+85196580 MsaT005781.1:CDS
ATTCCCTGTCCAAAAGCTGA+AGG 0.611859 1:+85196413 MsaT005781.1:CDS
ATTTGGAGAGCTCAACGTCA+TGG 0.614643 1:-85195605 None:intergenic
CCTGCTTTAAAGGAGAGCAG+TGG 0.616732 1:-85198969 None:intergenic
TGGAGAAGTTGGAGGAAGCA+AGG 0.617868 1:+85206860 MsaT005781.1:CDS
GGGTGGCATGCAAATGATGA+TGG 0.617923 1:+85196386 MsaT005781.1:CDS
GAGATAGAAGCAAAGAGACA+AGG 0.619116 1:+85195690 MsaT005781.1:CDS
TATACACAATAACACAAGGA+AGG 0.620376 1:+85196604 MsaT005781.1:CDS
GAGTAAGGCTGAAATCGAGA+AGG 0.620431 1:+85194233 MsaT005781.1:CDS
CCATTCAGGAATCTCGGGCA+GGG 0.621141 1:-85196354 None:intergenic
TCGGGCAGGGCAGCAGCACG+AGG 0.621316 1:-85196341 None:intergenic
ACAAAGGAAGAAACGAGTAA+AGG 0.621927 1:+85194285 MsaT005781.1:CDS
TTCATGGTGGAAAAGAAGAG+AGG 0.621997 1:+85196303 MsaT005781.1:CDS
TTGCAATTACCACAGCACAC+TGG 0.624403 1:-85195432 None:intergenic
TCAGAGAAATGACAAGGGCA+GGG 0.624776 1:+85195121 MsaT005781.1:CDS
TCATGGTGGAAAAGAAGAGA+GGG 0.625856 1:+85196304 MsaT005781.1:CDS
GAGGCCAATTTAGCCAATCA+GGG 0.627515 1:-85197889 None:intergenic
TGAAGTGAAATTGAAATCTG+AGG 0.628039 1:+85198681 MsaT005781.1:CDS
TTGTACCAATTGTTTAAACA+GGG 0.628105 1:-85195185 None:intergenic
GAAGTGAAATTGAAATCTGA+GGG 0.629648 1:+85198682 MsaT005781.1:CDS
CATGAAGATAATCACGCCCT+CGG 0.633290 1:-85196286 None:intergenic
TGTCCAAAAGCTGAAGGTGA+TGG 0.639166 1:+85196419 MsaT005781.1:CDS
AGAGGATGTTCCTAAGTTGG+AGG 0.639258 1:+85198909 MsaT005781.1:CDS
GCATAAGAGGAGGCTTAAGG+AGG 0.640950 1:+85204790 MsaT005781.1:CDS
CTACATGGCGATAATCAGGA+TGG 0.644609 1:+85198760 MsaT005781.1:CDS
ATCATCAATCCATAACCAGA+GGG 0.645093 1:-85206904 None:intergenic
GCCGTTAAAGGTTGATGACG+TGG 0.646340 1:+85194044 MsaT005781.1:CDS
GAGGCATGTCCAGTGTGCTG+TGG 0.646992 1:+85195423 MsaT005781.1:CDS
TGATCTGGTGGAGAAGTTGG+AGG 0.647545 1:+85206852 MsaT005781.1:CDS
CAATCAACAGTGGATCGAAG+AGG 0.650173 1:+85195076 MsaT005781.1:intron
TTTGTGTCTCCTGAAAATGT+TGG 0.652340 1:+85205958 MsaT005781.1:CDS
TGTTGCTGTGAGCTTCGTGG+CGG 0.653472 1:+85196218 MsaT005781.1:CDS
TCAATGTCAGAGAAATGACA+AGG 0.658738 1:+85195115 MsaT005781.1:CDS
GAGTAAAGGTGATGATGACA+TGG 0.658746 1:+85194299 MsaT005781.1:CDS
GCACCAAGTCCGAAATCTGA+AGG 0.659826 1:+85204981 MsaT005781.1:CDS
AGCGTTTGAGAACGGAACAA+AGG 0.661170 1:+85194269 MsaT005781.1:CDS
CGAAAAGGAAAATAAAACAG+GGG 0.664314 1:+85194386 MsaT005781.1:CDS
GATGTTCTAAAGCCAGACAT+GGG 0.664375 1:+85198061 MsaT005781.1:CDS
GCCACTCCGGCAAAGAAACG+CGG 0.668248 1:+85194348 MsaT005781.1:CDS
AAAAGTGCTGATCATGGATG+GGG 0.670907 1:-85204754 None:intergenic
CATCCGGCAACTTCACAGCA+AGG 0.672248 1:-85198039 None:intergenic
TATCATCAATCCATAACCAG+AGG 0.676710 1:-85206905 None:intergenic
GCGGGTGAAGAAAACAACAC+TGG 0.677579 1:+85194009 MsaT005781.1:CDS
AATGCACGCCACATGACAAG+CGG 0.682465 1:-85196785 None:intergenic
GAAAGAGGAGAAAATTGCTG+AGG 0.682762 1:+85195404 MsaT005781.1:CDS
TGATTGCTTAGATTGGTGCG+AGG 0.685495 1:+85196859 MsaT005781.1:CDS
TGACTATCGAGCATAAGAGG+AGG 0.692197 1:+85204780 MsaT005781.1:CDS
GGGCGTGATTATCTTCATGG+TGG 0.696150 1:+85196290 MsaT005781.1:CDS
AGGATATACACAATAACACA+AGG 0.701108 1:+85196600 MsaT005781.1:CDS
ATGAACTCAGCACAATGACG+AGG 0.704061 1:-85197965 None:intergenic
CGTGGCGGAAAGCTTCTAGG+TGG 0.704429 1:+85196233 MsaT005781.1:CDS
TCAACGTCACTGGAGCAAAG+GGG 0.706731 1:+85196709 MsaT005781.1:CDS
TTCTGATGAAGGTGATGACG+TGG 0.723150 1:+85194140 MsaT005781.1:CDS
CAATGTCAGAGAAATGACAA+GGG 0.723230 1:+85195116 MsaT005781.1:CDS
GTTCCAGTCATGGAAAATGG+GGG 0.732208 1:+85198847 MsaT005781.1:CDS
AATCGAGAAGGAGAAGACGG+AGG 0.733811 1:+85194245 MsaT005781.1:CDS
CTGAATGGTCAAGATCTGGG+TGG 0.752133 1:+85196369 MsaT005781.1:CDS
GGTAAATCACAGGTCCACAG+AGG 0.770669 1:+85206020 MsaT005781.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 85194006 85206936 85194006 ID=MsaG005781
Chr1 mRNA 85194006 85206936 85194006 ID=MsaT005781.1;Parent=MsaG005781
Chr1 exon 85194006 85194965 85194006 ID=MsaT005781.1.exon1;Parent=MsaT005781.1
Chr1 CDS 85194006 85194965 85194006 ID=cds.MsaT005781.1;Parent=MsaT005781.1
Chr1 exon 85195086 85195201 85195086 ID=MsaT005781.1.exon2;Parent=MsaT005781.1
Chr1 CDS 85195086 85195201 85195086 ID=cds.MsaT005781.1;Parent=MsaT005781.1
Chr1 exon 85195392 85195491 85195392 ID=MsaT005781.1.exon3;Parent=MsaT005781.1
Chr1 CDS 85195392 85195491 85195392 ID=cds.MsaT005781.1;Parent=MsaT005781.1
Chr1 exon 85195573 85195711 85195573 ID=MsaT005781.1.exon4;Parent=MsaT005781.1
Chr1 CDS 85195573 85195711 85195573 ID=cds.MsaT005781.1;Parent=MsaT005781.1
Chr1 exon 85196003 85196066 85196003 ID=MsaT005781.1.exon5;Parent=MsaT005781.1
Chr1 CDS 85196003 85196066 85196003 ID=cds.MsaT005781.1;Parent=MsaT005781.1
Chr1 exon 85196145 85196880 85196145 ID=MsaT005781.1.exon6;Parent=MsaT005781.1
Chr1 CDS 85196145 85196880 85196145 ID=cds.MsaT005781.1;Parent=MsaT005781.1
Chr1 exon 85197842 85198171 85197842 ID=MsaT005781.1.exon7;Parent=MsaT005781.1
Chr1 CDS 85197842 85198171 85197842 ID=cds.MsaT005781.1;Parent=MsaT005781.1
Chr1 exon 85198658 85198990 85198658 ID=MsaT005781.1.exon8;Parent=MsaT005781.1
Chr1 CDS 85198658 85198990 85198658 ID=cds.MsaT005781.1;Parent=MsaT005781.1
Chr1 exon 85204746 85204818 85204746 ID=MsaT005781.1.exon9;Parent=MsaT005781.1
Chr1 CDS 85204746 85204818 85204746 ID=cds.MsaT005781.1;Parent=MsaT005781.1
Chr1 exon 85204917 85205002 85204917 ID=MsaT005781.1.exon10;Parent=MsaT005781.1
Chr1 CDS 85204917 85205002 85204917 ID=cds.MsaT005781.1;Parent=MsaT005781.1
Chr1 exon 85205934 85206053 85205934 ID=MsaT005781.1.exon11;Parent=MsaT005781.1
Chr1 CDS 85205934 85206053 85205934 ID=cds.MsaT005781.1;Parent=MsaT005781.1
Chr1 exon 85206823 85206936 85206823 ID=MsaT005781.1.exon12;Parent=MsaT005781.1
Chr1 CDS 85206823 85206936 85206823 ID=cds.MsaT005781.1;Parent=MsaT005781.1
Gene Sequence

>MsaT005781.1

ATGGCGGGTGAAGAAAACAACACTGGTGCTGAAACGGTGCCGTTAAAGGTTGATGACGTGGCACAAGAATCAGTTCCTGATCGTGAAAAACAAGCTATTGTCTCCGAAACGGCGTCGTTGAAGGACGATTTAGCTTCTGATGAAGGTGATGACGTGGCAGCCGAAGATTCTTCTTCCGTTAAAGTGATAAGAAAATACGGTAGAAGGAAAAAGAAACCAGGAAGAAAGAGTAAGGCTGAAATCGAGAAGGAGAAGACGGAGGAAGCGTTTGAGAACGGAACAAAGGAAGAAACGAGTAAAGGTGATGATGACATGGCAGCCGGAAACTCTATTTCCGTTCATGCCACTCCGGCAAAGAAACGCGGTAGAAATACAAAAACCGAAAAGGAAAATAAAACAGGGGAAGGTGCTGATGTGGTAGGAGGGTTTTCTTCTGTTGGCGTGAGGAAGAGTGAAAGGCCTAGGAAGATTAAATCATTGAATGAAGATTACTTGTCGGATTTGGATGAGGCTGTGAAGAAAAAGGGGAAACGTGGTAGGAAGAAGAAGGTCATAATCGGTGTTTCTGATGAAAGTGATGAAGCTGAACATAAAGGGGAAAATGTGAAGACAGAGAAGAAGAAACCTGGTAGGAAAAGGAAGGAAATTTCTAGTTCTGGAGATGAAAATGAAGCTGAAAAAGAGGGAGTAAATGTCAAAAAAGAGGAAAAGAAAAAACCTGGTAGAAAAAAGAAGGTAAGTCATAATAGCTCTGAAGAAAATGATGAAGATGAACAAGATGGGGAAAATGGTAGAACTGTTAAGATTGAGGAGAATGAACTGACCAGTGAAAAGAATTTGGAGAGTAGTGTTTTATCCGATGATATTAAAGGTTATTCTTTAAGAACGGCTAAAAAAATCAAGCCTGAATGCATTGAACAGCATCAAACGGTCGCAAAGTTCAACAAGAGAAATTCTAAGTGGATCGAAGAGGAATCCTTGATGTGCCATCAATGTCAGAGAAATGACAAGGGCAGGGTTGTCAGATGCACAAAATGCAAACGGAAAAGATATTGCATACCCTGTTTAAACAATTGGTATCCTCACTTGAAAGAGGAGAAAATTGCTGAGGCATGTCCAGTGTGCTGTGGTAATTGCAATTGCAAAGCTTGCTTGCGATCATCTGTACTTATCAAGGAAATTAAAAATAAGACGGAAACCAACAACGACCATGACGTTGAGCTCTCCAAATATATGCTGAAAGTACTTCTTCCATATTTAAGACGTTTGGATGAAGAGCAAATGGCTGAGAAGGAGATAGAAGCAAAGAGACAAGGGCGCTCCCTTTCTGAGCTAAAAATAGAAGTTGCAGATATCCCTAAAAAGGAGCGTGTGTACTGTGACATCTGCAAAACGTCAATATTCGACTACCACAGAAGCTGTGCAAAATGCTCTTTTGACATTTGTCTCCTTTGTTGCTGTGAGCTTCGTGGCGGAAAGCTTCTAGGTGGTGCAGATCCAATTGAGTTTGAGTTTATCTTCCGAGGGCGTGATTATCTTCATGGTGGAAAAGAAGAGAGGGTAAAAAAAAAAGAACCTCGTGCTGCTGCCCTGCCCGAGATTCCTGAATGGTCAAGATCTGGGTGGCATGCAAATGATGATGGTAGCATTCCCTGTCCAAAAGCTGAAGGTGATGGTGATCATGGTGTCCTTGAACTGAGAAGCACACTTCCACCAAATTGCATCTCTGAGTTACTATGTAAAGCAAAAGAACTTGAAGCAACAATTGCGCTTCAAGATGCAAAAGAGACTTTTGATAGTTGGTGTTCTTGTTTAAAGCCTGTTAGGAATGAAGAGGATATACACAATAACACAAGGAAGGCAGCTTCTCGTGAAGATTCCAGTGATAACATTTTATACTGTCCCAGGGCTGTAAATCTACATAATGAGGATTTGCGACATTTTCAACGTCACTGGAGCAAAGGGGAGCCTGTAATTGTTAGCAATGTACTTGAATGTACATCTGGTTTAAGCTGGGAACCGCTTGTCATGTGGCGTGCATTCCGTCAGATAAGCAATACCAAGCACAAAACACTTTTGGATGTGAAAGCTATTGATTGCTTAGATTGGTGCGAGGGAGATATTAATGTGCACCAATTCTTTACTGGGTACACAAAAGGTCGCCCTGATTGGCTAAATTGGCCTCAGGTTTTGAAATTGAAAGATTGGCCTCCTTCTAACTTATTTGAAGAAAGTTTGCCTCGTCATTGTGCTGAGTTCATATCTTCCTTGCCCTATAAAGAATATACAGATCCTTTCAAAGGTGTTCTTAACCTTGCTGTGAAGTTGCCGGATGATGTTCTAAAGCCAGACATGGGCCCTAAAACATATATTGCTTATGGATTTGAGCAGGAACTTGGGCGGGGTGATTCTGTGACTAAGCTTCATTGTGATATGTCTGATGCAGTAAATGTGCTGACTCATATTGCTGAAGTGAAATTGAAATCTGAGGGCATTACTGCCATTAAGAAACTGACACAAAAGCACCGTGAGCAAGACAAGAGGGAGCTACATGGCGATAATCAGGATGGAGAGACCACTATCATGCTTGATAATTCATCTTCTTCCATAAATGCTTCAGACGAAAAAAATTGCGTTCCAGTCATGGAAAATGGGGGTGATTCCTTAGACGGTGCTCTCTGGGATATTTTTCGGAGAGAGGATGTTCCTAAGTTGGAGGAATATTTAAAGAAGCATTTCAGAGAGTTCAGGCATGTCCACTGCTCTCCTTTAAAGCAGGTTATTCACCCCATCCATGATCAGCACTTTTATTTGACTATCGAGCATAAGAGGAGGCTTAAGGAGGAGTATGGAATTGAACCTTGGACTTTTTTCCAGAAGCTAGGAGATGCTGTTTTCATTCCAGCTGGTTGTCCGCACCAAGTCCGAAATCTGAAGTCATGCACCAAGGTTGCTCTTGATTTTGTGTCTCCTGAAAATGTTGGCGAGTGCTTTCGTTTGACAGAGGAATTCCGTAAACTTCCGGTAAATCACAGGTCCACAGAGGACAAATTGGAGGTGAAGAAAATGATTATATATGCTATGCTTGATCTGGTGGAGAAGTTGGAGGAAGCAAGGTTAGTGGTTGCAAACTTCACGCCCTCTGGTTATGGATTGATGATACTTATTTGA
Protein sequence

>MsaT005781.1

MAGEENNTGAETVPLKVDDVAQESVPDREKQAIVSETASLKDDLASDEGDDVAAEDSSSVKVIRKYGRRKKKPGRKSKAEIEKEKTEEAFENGTKEETSKGDDDMAAGNSISVHATPAKKRGRNTKTEKENKTGEGADVVGGFSSVGVRKSERPRKIKSLNEDYLSDLDEAVKKKGKRGRKKKVIIGVSDESDEAEHKGENVKTEKKKPGRKRKEISSSGDENEAEKEGVNVKKEEKKKPGRKKKVSHNSSEENDEDEQDGENGRTVKIEENELTSEKNLESSVLSDDIKGYSLRTAKKIKPECIEQHQTVAKFNKRNSKWIEEESLMCHQCQRNDKGRVVRCTKCKRKRYCIPCLNNWYPHLKEEKIAEACPVCCGNCNCKACLRSSVLIKEIKNKTETNNDHDVELSKYMLKVLLPYLRRLDEEQMAEKEIEAKRQGRSLSELKIEVADIPKKERVYCDICKTSIFDYHRSCAKCSFDICLLCCCELRGGKLLGGADPIEFEFIFRGRDYLHGGKEERVKKKEPRAAALPEIPEWSRSGWHANDDGSIPCPKAEGDGDHGVLELRSTLPPNCISELLCKAKELEATIALQDAKETFDSWCSCLKPVRNEEDIHNNTRKAASREDSSDNILYCPRAVNLHNEDLRHFQRHWSKGEPVIVSNVLECTSGLSWEPLVMWRAFRQISNTKHKTLLDVKAIDCLDWCEGDINVHQFFTGYTKGRPDWLNWPQVLKLKDWPPSNLFEESLPRHCAEFISSLPYKEYTDPFKGVLNLAVKLPDDVLKPDMGPKTYIAYGFEQELGRGDSVTKLHCDMSDAVNVLTHIAEVKLKSEGITAIKKLTQKHREQDKRELHGDNQDGETTIMLDNSSSSINASDEKNCVPVMENGGDSLDGALWDIFRREDVPKLEEYLKKHFREFRHVHCSPLKQVIHPIHDQHFYLTIEHKRRLKEEYGIEPWTFFQKLGDAVFIPAGCPHQVRNLKSCTKVALDFVSPENVGECFRLTEEFRKLPVNHRSTEDKLEVKKMIIYAMLDLVEKLEEARLVVANFTPSGYGLMILI*