AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005798


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005798 MtrunA17_Chr1g0211941 97.692 260 2 2 1 259 1 257 0.0 508
MsaG005798 MtrunA17_Chr1g0164881 61.111 252 53 7 17 257 16 233 1.83e-79 239
MsaG005798 MtrunA17_Chr5g0416581 65.217 115 29 3 7 114 39 149 2.59e-42 148
MsaG005798 MtrunA17_Chr2g0277361 74.737 95 21 1 26 117 121 215 3.67e-41 146
MsaG005798 MtrunA17_Chr7g0224581 65.000 100 28 2 19 118 37 129 3.15e-37 132
MsaG005798 MtrunA17_Chr4g0060601 75.309 81 15 1 26 101 86 166 2.19e-36 134
MsaG005798 MtrunA17_Chr1g0180911 73.418 79 17 2 19 94 34 111 8.32e-35 124
MsaG005798 MtrunA17_Chr1g0204001 62.626 99 31 2 10 106 40 134 1.19e-32 122
MsaG005798 MtrunA17_Chr8g0350941 70.130 77 23 0 28 104 76 152 1.29e-31 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005798 AT5G23280.1 64.961 254 56 9 12 257 20 248 2.57e-96 283
MsaG005798 AT5G08330.1 55.351 271 75 8 1 259 1 237 5.69e-77 233
MsaG005798 AT1G58100.1 77.907 86 19 0 33 118 61 146 7.17e-41 145
MsaG005798 AT1G35560.1 74.444 90 23 0 29 118 52 141 2.82e-39 139
MsaG005798 AT1G72010.1 78.571 84 17 1 26 109 58 140 2.87e-39 140
MsaG005798 AT3G47620.1 76.829 82 14 1 25 101 105 186 1.88e-37 137
MsaG005798 AT1G69690.1 75.949 79 14 1 28 101 43 121 8.59e-37 132
MsaG005798 AT3G27010.2 75.342 73 18 0 21 93 67 139 2.21e-33 123
MsaG005798 AT3G27010.1 75.342 73 18 0 21 93 67 139 2.21e-33 123
MsaG005798 AT5G51910.1 65.517 87 30 0 18 104 43 129 6.16e-33 122
MsaG005798 AT5G51910.2 65.517 87 30 0 18 104 43 129 6.16e-33 122
MsaG005798 AT2G45680.1 62.366 93 28 1 27 119 70 155 5.00e-32 120
MsaG005798 AT5G41030.1 66.176 68 23 0 28 95 64 131 8.73e-28 107
MsaG005798 AT2G37000.1 76.271 59 14 0 33 91 44 102 7.62e-27 103
MsaG005798 AT3G45150.1 42.742 124 47 4 21 122 8 129 7.85e-21 86.7
MsaG005798 AT1G58100.2 54.651 86 15 1 33 118 61 122 1.58e-19 87.0

Find 80 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAACACCGGCGGCTCTT+TGG 0.201653 1:+85324018 MsaT005798.1:CDS
AGGAGAAGGGAGAGAGTTAT+TGG 0.218287 1:-85323868 None:intergenic
TTACCTGCACGGCCTGATTT+TGG 0.227364 1:+85324044 MsaT005798.1:CDS
TTCAACACCGGCGGCTCTTT+GGG 0.244327 1:+85324019 MsaT005798.1:CDS
CGGCTAGTTGTTGACGGTTT+GGG 0.317306 1:-85323608 None:intergenic
TCTCCTACGCCGTTCATTCT+CGG 0.325836 1:+85323924 MsaT005798.1:CDS
AATTTGCTTGCTTCGTTGTC+TGG 0.328042 1:+85324266 MsaT005798.1:CDS
TTCCAGCTCACACGCGAATT+AGG 0.343017 1:+85323720 MsaT005798.1:CDS
AGCTTTGCTGCTGCTGCCGC+CGG 0.352446 1:+85324077 MsaT005798.1:CDS
ACGGCTAGTTGTTGACGGTT+TGG 0.367652 1:-85323609 None:intergenic
TCTCTCTCCGTTTCACTTCG+TGG 0.367948 1:+85323843 MsaT005798.1:CDS
TCTGGTGGACATGGAAATTC+TGG 0.390225 1:+85324284 MsaT005798.1:CDS
TGCTCCTCCGCCGGAAATGA+TGG 0.394211 1:+85324100 MsaT005798.1:CDS
GTTGTTGTTGTGGTTCCGTC+GGG 0.397707 1:-85323561 None:intergenic
ACAACAGCATCAACAAGTTA+TGG 0.406472 1:+85324196 MsaT005798.1:CDS
GGTACCGGTGGCGGCGATGA+TGG 0.418821 1:-85323796 None:intergenic
AAAGACTGAACCATCATTTC+CGG 0.426674 1:-85324110 None:intergenic
GAGAGAGAAGAGAAACTTGC+CGG 0.430775 1:-85323828 None:intergenic
ACACGTGCGGCGCATATGAT+TGG 0.431664 1:-85323696 None:intergenic
GTGAAGTGGAGGAAGGAGAA+GGG 0.432577 1:-85323881 None:intergenic
CTCCAAATTTGACCAAAATC+AGG 0.435338 1:-85324056 None:intergenic
CTCTTTGGGCTTTACCTGCA+CGG 0.438680 1:+85324033 MsaT005798.1:CDS
TGCCGCCGGTGCTCCTCCGC+CGG 0.444737 1:+85324091 MsaT005798.1:CDS
AAAGATGATGCTCTTTCTGT+TGG 0.447514 1:+85323990 MsaT005798.1:CDS
CAACAGCATCAACAAGTTAT+GGG 0.456904 1:+85324197 MsaT005798.1:CDS
TGCCGGAGTTGTGCCGGTAC+CGG 0.460326 1:-85323811 None:intergenic
AGTGAAGTGGAGGAAGGAGA+AGG 0.462868 1:-85323882 None:intergenic
ATCATCGCCGCCACCGGTAC+CGG 0.463092 1:+85323798 MsaT005798.1:CDS
TTTGTCTCAGCAGCAACATC+AGG 0.466168 1:+85324130 MsaT005798.1:CDS
ATATGATTGGCATACGAATA+CGG 0.468808 1:-85323683 None:intergenic
AGAGTTGGGAATTATCTTCC+TGG 0.477725 1:+85324236 MsaT005798.1:CDS
AGTTGTGCCGGTACCGGTGG+CGG 0.478006 1:-85323805 None:intergenic
GGTTGTTGTTGTGGTTCCGT+CGG 0.488997 1:-85323562 None:intergenic
CACAGTAAAGTCGACGGCCG+TGG 0.489495 1:+85323657 MsaT005798.1:CDS
ACCGGTGGCGGCGATGATGG+AGG 0.492286 1:-85323793 None:intergenic
TGGTCGAGTGAAGTGGAGGA+AGG 0.494112 1:-85323888 None:intergenic
GACCTAATTCGCGTGTGAGC+TGG 0.494851 1:-85323722 None:intergenic
GTTCGTGGTGGTTGTTGTTG+TGG 0.498533 1:-85323571 None:intergenic
CATTTCCGGCGGAGGAGCAC+CGG 0.501079 1:-85324096 None:intergenic
GCTTCGTTGTCTGGTGGACA+TGG 0.503221 1:+85324275 MsaT005798.1:CDS
CCCTCCATCATCGCCGCCAC+CGG 0.503587 1:+85323792 MsaT005798.1:CDS
GAAGCTTCAGCTGCTAGAGT+TGG 0.504823 1:+85324221 MsaT005798.1:CDS
AAGCTTCAGCTGCTAGAGTT+GGG 0.504829 1:+85324222 MsaT005798.1:CDS
TGATGGAGGGTTCAGCTTGA+CGG 0.505105 1:-85323779 None:intergenic
TGACCAAAATCAGGCCGTGC+AGG 0.513660 1:-85324047 None:intergenic
CACCGGTACCGGCACAACTC+CGG 0.514457 1:+85323809 MsaT005798.1:CDS
TAGGAGAGAGAAGCGGTTTG+TGG 0.517147 1:-85323908 None:intergenic
TTGCTTGCTTCGTTGTCTGG+TGG 0.525229 1:+85324269 MsaT005798.1:CDS
TGAACCATCATTTCCGGCGG+AGG 0.529418 1:-85324104 None:intergenic
CCATTCGATGGTTTGACCGT+CGG 0.532798 1:-85323751 None:intergenic
GGTTCCGTCGGGGTTAGACA+TGG 0.533278 1:-85323550 None:intergenic
CCGGTGGCGGCGATGATGGA+GGG 0.534932 1:-85323792 None:intergenic
TGTTGGTTCTCTTTCAACAC+CGG 0.535689 1:+85324007 MsaT005798.1:CDS
GTCAATGATTGAACCGTTCG+TGG 0.543005 1:-85323586 None:intergenic
ATTGGAGCCACGAAGTGAAA+CGG 0.550877 1:-85323850 None:intergenic
TTCCGGCGGAGGAGCACCGG+CGG 0.553371 1:-85324093 None:intergenic
GAAACTTGCCGGAGTTGTGC+CGG 0.558406 1:-85323817 None:intergenic
CGGTTTGTGGTCGAGTGAAG+TGG 0.560737 1:-85323895 None:intergenic
GCAAGCAAATTGAGATGACC+AGG 0.567406 1:-85324254 None:intergenic
GCATACGAATACGGCGTCCA+CGG 0.572506 1:-85323674 None:intergenic
TTGACGGAGGAGCCATTCGA+TGG 0.578156 1:-85323763 None:intergenic
CGGAGTTGTGCCGGTACCGG+TGG 0.578615 1:-85323808 None:intergenic
ACAACAACAACCACCACGAA+CGG 0.583850 1:+85323573 MsaT005798.1:CDS
GGTAAAGCCCAAAGAGCCGC+CGG 0.587790 1:-85324026 None:intergenic
ATACGTTTACCGAGAATGAA+CGG 0.599547 1:-85323933 None:intergenic
GGCCGTCGACTTTACTGTGA+CGG 0.604244 1:-85323653 None:intergenic
CCGACGGTCAAACCATCGAA+TGG 0.640967 1:+85323751 MsaT005798.1:CDS
TTTGTGGTCGAGTGAAGTGG+AGG 0.647959 1:-85323892 None:intergenic
TTACCGAGAATGAACGGCGT+AGG 0.649539 1:-85323927 None:intergenic
GACTGAACCATCATTTCCGG+CGG 0.662606 1:-85324107 None:intergenic
GACCGTCACAGTAAAGTCGA+CGG 0.664072 1:+85323651 MsaT005798.1:CDS
TGGTTCTCTTTCAACACCGG+CGG 0.671866 1:+85324010 MsaT005798.1:CDS
GTGGACATGGAAATTCTGGT+CGG 0.673965 1:+85324288 MsaT005798.1:CDS
AATGATTGAACCGTTCGTGG+TGG 0.682160 1:-85323583 None:intergenic
TGTGAGCTGGAAGACACGTG+CGG 0.686113 1:-85323709 None:intergenic
AAAACCATGTCTAACCCCGA+CGG 0.687631 1:+85323546 None:intergenic
TTGTTGTTGTGGTTCCGTCG+GGG 0.691268 1:-85323560 None:intergenic
TGGAGGGTTCAGCTTGACGG+AGG 0.697001 1:-85323776 None:intergenic
GAATTAGGTCACAAATCCGA+CGG 0.701084 1:+85323735 MsaT005798.1:CDS
AACGGCGTAGGAGAGAGAAG+CGG 0.760443 1:-85323915 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 85323552 85324331 85323552 ID=MsaG005798
Chr1 mRNA 85323552 85324331 85323552 ID=MsaT005798.1;Parent=MsaG005798
Chr1 exon 85323552 85324331 85323552 ID=MsaT005798.1.exon1;Parent=MsaT005798.1
Chr1 CDS 85323552 85324331 85323552 ID=cds.MsaT005798.1;Parent=MsaT005798.1
Gene Sequence

>MsaT005798.1

ATGTCTAACCCCGACGGAACCACAACAACAACCACCACGAACGGTTCAATCATTGACCCAAACCGTCAACAACTAGCCGTCAAAAAACCGCCGGCAAAAGACCGTCACAGTAAAGTCGACGGCCGTGGACGCCGTATTCGTATGCCAATCATATGCGCCGCACGTGTCTTCCAGCTCACACGCGAATTAGGTCACAAATCCGACGGTCAAACCATCGAATGGCTCCTCCGTCAAGCTGAACCCTCCATCATCGCCGCCACCGGTACCGGCACAACTCCGGCAAGTTTCTCTTCTCTCTCCGTTTCACTTCGTGGCTCCAATAACTCTCTCCCTTCTCCTTCCTCCACTTCACTCGACCACAAACCGCTTCTCTCTCCTACGCCGTTCATTCTCGGTAAACGTATTCGCACTGATGATGATTCTTCTTCTTCTTCTGCTAAAGATGATGCTCTTTCTGTTGGTTCTCTTTCAACACCGGCGGCTCTTTGGGCTTTACCTGCACGGCCTGATTTTGGTCAAATTTGGAGCTTTGCTGCTGCTGCCGCCGGTGCTCCTCCGCCGGAAATGATGGTTCAGTCTTTGTCTCAGCAGCAACATCAGGTTCAAGCTAATTTCTTTGCTCATCATCATCAGCAGCAACAGCAACAACAGCATCAACAAGTTATGGGTGAAGCTTCAGCTGCTAGAGTTGGGAATTATCTTCCTGGTCATCTCAATTTGCTTGCTTCGTTGTCTGGTGGACATGGAAATTCTGGTCGGAGAGATGATGAACATCATTGA

Protein sequence

>MsaT005798.1

MSNPDGTTTTTTTNGSIIDPNRQQLAVKKPPAKDRHSKVDGRGRRIRMPIICAARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPASFSSLSVSLRGSNNSLPSPSSTSLDHKPLLSPTPFILGKRIRTDDDSSSSSAKDDALSVGSLSTPAALWALPARPDFGQIWSFAAAAAGAPPPEMMVQSLSQQQHQVQANFFAHHHQQQQQQQHQQVMGEASAARVGNYLPGHLNLLASLSGGHGNSGRRDDEHH*